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<document id="87F867238B70D5C4B551A4724521609B" ID-DOI="10.5281/zenodo.10396362" ID-ISSN="1942-1354" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1707168658757" checkinUser="felipe" docAuthor="Zhang, Jing, Cong, Qian &amp; Grishin, Nick V." docDate="2023" docId="03810139FF88BB07C0CAFEA2E1C1B425" docLanguage="en" docName="InsectaMundi.2023.1026.1-115.pdf" docOrigin="Insecta Mundi 2023 (26)" docSource="http://dx.doi.org/10.5281/zenodo.10396362" docStyle="DocumentStyle:F9976E9B7F8A094D890004515A7A0592.3:InsectaMundi.2020-.journal_article" docStyleId="F9976E9B7F8A094D890004515A7A0592" docStyleName="InsectaMundi.2020-.journal_article" docStyleVersion="3" docTitle="Tromba xantha Zhang &amp; Cong &amp; Grishin 2023, new species" docType="treatment" docVersion="1" lastPageNumber="86" masterDocId="FFB87941FFDFBB50C00AFF56E355B262" masterDocTitle="Supplementary Materials and Appendix" masterLastPageNumber="115" masterPageNumber="1" pageNumber="86" updateTime="1707169288913" updateUser="GgImagineBatch" zenodo-license-document="CC-BY-NC-3.0">
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<mods:title id="6D5E75CBF8427F2A762591A1E85C05B4">Supplementary Materials and Appendix</mods:title>
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<mods:namePart id="CF3EA7136957CA0C2738F20BF90A03C9">Zhang, Jing</mods:namePart>
<mods:affiliation id="5D87A901F65B2BCCB665594424DB8827">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="FF4B12FAA819842AA8AA6A0C43503B65">Cong, Qian</mods:namePart>
<mods:affiliation id="9828D023CADB3D787D48A889B0FBB224">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="8C3EC10A1F72554F9B24E0AD14E86EFB">Grishin, Nick V.</mods:namePart>
<mods:affiliation id="FEA8DDABE527A171C48A7447DE1AF37F">Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA</mods:affiliation>
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<mods:title id="49B90DFDC919FACA66CBBEB4E58D1B37">Insecta Mundi</mods:title>
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<mods:date id="44CCB20767029A4E128DAC51B31C7201">2023</mods:date>
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<taxonomicName id="4C28CBACFF88BB07C0CAFEA2E2BBB073" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[192,494,500,529]" class="Insecta" family="Hesperiidae" genus="Tromba" kingdom="Animalia" order="Lepidoptera" pageId="87" pageNumber="86" phylum="Arthropoda" rank="species" species="xantha" status="sp. nov.">
<emphasis id="B95C6C3DFF88BB07C0CAFEA2E2D6B073" bold="true" box="[192,387,500,529]" italics="true" pageId="87" pageNumber="86">Tromba xantha</emphasis>
Grishin
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,
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https://zoobank.org/
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(
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part, 213214, 454455)
</paragraph>
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<subSubSection id="C332E3A4FF88BB07C0CAFD32E7D8B6EC" pageId="87" pageNumber="86" type="diagnosis">
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<emphasis id="B95C6C3DFF88BB07C0CAFD32E2BFB01C" bold="true" box="[192,490,612,638]" pageId="87" pageNumber="86">Definition and diagnosis.</emphasis>
Phylogenetic trees reveal that a number of specimens identified as
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<emphasis id="B95C6C3DFF88BB07C4D4FD32E6F4B01C" box="[1246,1441,612,638]" italics="true" pageId="87" pageNumber="86">Tromba xanthura</emphasis>
(Godman, 1901)
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(type locality in
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) show prominent genetic differentiation from it (
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): e.g., their COI barcodes differ by 3.6% (24 bp), and therefore represent a new species. This new species keys to
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<emphasis id="B95C6C3DFF88BB07C515FDFEE6F5B0A0" box="[1311,1440,680,706]" italics="true" pageId="87" pageNumber="86">T. xanthura</emphasis>
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(K.14.2) in
<bibRefCitation id="EFB9CDDEFF88BB07C14FFD9CE28CB086" author="Evans WH" box="[325,473,713,740]" pageId="87" pageNumber="86" pagination="54 - 88" refId="ref77169" refString="Evans WH. 1955. A catalogue of the American Hesperiidae indicating the classification and nomenclature adopted in the British Museum (Natural History). Part IV. Hesperiinae and Megathyminae. The Trustees of the British Museum (Natural History); London. v + 499 p., pl. 54 - 88." type="book chapter" year="1955">Evans (1955)</bibRefCitation>
but differs from it by male genitalia with deeper separation of harpe from ampulla, which is only slightly notched in
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<emphasis id="B95C6C3DFF88BB07C23AFDBAE1E4B167" box="[560,689,747,773]" italics="true" pageId="87" pageNumber="86">T. xanthura</emphasis>
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, but in some specimens of the new species (including the
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,
<figureCitation id="1313ACAAFF88BB07C0CAFC5BE201B14A" box="[192,340,781,808]" captionStart="Figures 451467" captionStartId="116.[192,271,1780,1804]" captionTargetBox="[193,1440,277,1733]" captionTargetId="figure-107@116.[192,1440,264,1737]" captionTargetPageId="116" captionText="Figures 451467. Genitalia of holotypes (top) and spread specimens (bottom), data in text. 451453) Damas zea sp. n. 454455) Tromba xantha sp. n. 456458) Perichares fura sp. n. 459464) Carystoides (Balma) goliath sp. n. Views: dorsal (454, 459 valvae and aedeagus removed, 462 valvae), left lateral (455, 463 right valva, 464 both valvae, 461 aedeagus), ventral (451, 453, 456, 458), left ventrolateral (452), right ventrolateral (457). Smaller scales refer to images next to them. 465466) Neotype of Eudamus briccius. 467) A drawing of E. briccius in ZSMC (with a smaller scale)." pageId="87" pageNumber="86">Fig. 454455</figureCitation>
) is separated by a groove, more prominent serrations towards distal end of the dorsal margin of harpe, and less bulky ventral margin on harpe near its fusion with the body of valva. Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly
<date id="FF9696EFFF88BB07C26AFC25E184B1EF" box="[608,721,883,909]" pageId="87" pageNumber="86" value="2672-07-10">2672.7.10</date>
:C117T, aly
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:A156T, aly133.6.8:G260A, aly4592.2.4:G73A, aly4592.2.4:A74T, and COI barcode: 49G, 238C, T340C, T367C, T640C.
</paragraph>
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Barcode sequence of the
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.
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Sample NVG-5054, GenBank OR837720, 658 base pairs: AACTTTATATTTTATTTTCGGTATTTGAGCAGGAATATTAGGAACATCGTTAAGATTATTAATTCGAACTGAATTAGGTAATCCAGGATCTTTAATT GGAGATGATCAAATTTATAATACCATTGTAACAGCTCATGCCTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGTTTTGGAAATT GATTAGTACCATTAATATTAGGAGCACCAGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGAATATTACCTCCTTCATTAACTTTATTAAT CTCAAGAAGAATTGTAGAAAATGGTGCAGGAACAGGATGAACTGTTTACCCCCCTTTATCTTCTAATATTGCTCACCAAGGATCTTCAGTAGATTTA GCAATTTTTTCTCTTCATTTAGCAGGTATTTCTTCTATTTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGAATTATAAACTTATCTT TTGACCAAATACCTTTATTTGTTTGATCTGTAGGAATTACAGCTTTATTATTATTATTATCTTTACCTGTATTAGCTGGAGCTATTACAATATTACT TACTGATCGAAATTTAAATACTTCTTTTTTTGATCCTGCAGGAGGTGGAGATCCAATCTTATATCAACATTTATTT
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:
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deposited in the
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,
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(
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), illustrated in
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, bears the following five rectangular labels, four white: [Paso San Juan, |
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. Cruz.], [
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| xanthura | Godm. | comp. type.], [DNA sample ID: | NVG-5054 | c/o Nick
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. Grishin], [genitalia |
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| Nick
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. Grishin], and one red [
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|
<taxonomicName id="4C28CBACFF88BB07C542FA50E258B720" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" class="Insecta" family="Hesperiidae" genus="Tromba" kingdom="Animalia" order="Lepidoptera" pageId="87" pageNumber="86" phylum="Arthropoda" rank="species" species="xantha">Tromba xantha</taxonomicName>
| Grishin]
</materialsCitation>
.
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:
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:
<specimenCount id="9D2E7BA6FF88BB07C259FA7EE120B727" box="[595,629,1320,1349]" count="1" pageId="87" pageNumber="86" type="male">1♂</specimenCount>
NVG-17111B07
<collectingCountry id="F33FF0BFFF88BB07C33FFA7EE0DEB720" box="[821,907,1320,1346]" name="Mexico" pageId="87" pageNumber="86">Mexico</collectingCountry>
:
<collectingRegion id="49EC7ECDFF88BB07C39EFA7EE0A8B720" box="[916,1021,1320,1346]" country="Mexico" name="Guerrero" pageId="87" pageNumber="86">Guerrero</collectingRegion>
,
<location id="8EF7E6F4FF88BB07C40CFA7FE71BB721" LSID="urn:lsid:plazi:treatment:03810139FF88BB07C0CAFEA2E1C1B425:8EF7E6F4FF88BB07C40CFA7FE71BB721" box="[1030,1102,1321,1347]" country="Mexico" name="Ixtapa" pageId="87" pageNumber="86" stateProvince="Guerrero">Ixtapa</location>
,
<date id="FF9696EFFF88BB07C453FA7EE7B5B721" box="[1113,1248,1320,1347]" pageId="87" pageNumber="86" value="1985-03-05">
<collectingDate id="EFD26F07FF88BB07C453FA7EE7B5B721" box="[1113,1248,1320,1347]" pageId="87" pageNumber="86" value="1985-03-05">5-Mar-1985</collectingDate>
</date>
,
<collectorName id="26DDD5F9FF88BB07C4E0FA7EE3B1B706" pageId="87" pageNumber="86">Benjamin Landing</collectorName>
leg. [
<collectionCode id="ED3928EAFF88BB07C117FA1CE226B706" box="[285,371,1354,1380]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34802" lsid="urn:lsid:biocol.org:col:34802" name="Natural History Museum of Los Angeles County" pageId="87" pageNumber="86" type="Museum">LACM</collectionCode>
]
</materialsCitation>
;
<materialsCitation id="3B40BA72FF88BB07C18EFA1CE288B7E4" collectingDate="1980-04-17" collectionCode="USNM" collectorName="Robert D. Lehman" country="Honduras" location="La Ceiba" pageId="87" pageNumber="86" specimenCode="NVG151102-10" specimenCount="1" specimenCount-male="1" typeStatus="paratype">
<specimenCount id="9D2E7BA6FF88BB07C18EFA1CE2F3B705" box="[388,422,1354,1383]" count="1" pageId="87" pageNumber="86" type="male">1♂</specimenCount>
NVG-5055
<collectingCountry id="F33FF0BFFF88BB07C224FA1CE1F4B706" box="[558,673,1354,1380]" name="Honduras" pageId="87" pageNumber="86">Honduras</collectingCountry>
:
<quantity id="4CD01DCAFF88BB07C2A0FA1CE1A5B706" box="[682,752,1354,1380]" metricMagnitude="4" metricUnit="m" metricValue="1.8" pageId="87" pageNumber="86" unit="km" value="18.0">18 km</quantity>
W of
<location id="8EF7E6F4FF88BB07C33EFA1CE0CCB706" LSID="urn:lsid:plazi:treatment:03810139FF88BB07C0CAFEA2E1C1B425:8EF7E6F4FF88BB07C33EFA1CE0CCB706" box="[820,921,1354,1380]" country="Honduras" name="La Ceiba" pageId="87" pageNumber="86">La Ceiba</location>
,
<date id="FF9696EFFF88BB07C3A9FA1CE767B706" box="[931,1074,1354,1380]" pageId="87" pageNumber="86" value="1980-04-17">
<collectingDate id="EFD26F07FF88BB07C3A9FA1CE767B706" box="[931,1074,1354,1380]" pageId="87" pageNumber="86" value="1980-04-17">17-Apr-1980</collectingDate>
</date>
<collectorName id="26DDD5F9FF88BB07C432FA1CE652B706" box="[1080,1287,1354,1380]" pageId="87" pageNumber="86">Robert D. Lehman</collectorName>
leg., genitalia
<specimenCode id="DB8E1854FF88BB07C0CAFA3AE224B7E5" box="[192,369,1388,1415]" collectionCode="NVG" pageId="87" pageNumber="86">NVG151102-10</specimenCode>
[
<collectionCode id="ED3928EAFF88BB07C174FA3AE283B7E4" box="[382,470,1388,1414]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="87" pageNumber="86" type="Museum">USNM</collectionCode>
]
</materialsCitation>
;
<materialsCitation id="3B40BA72FF88BB07C1E2FA3AE141B7CA" collectionCode="USNM" collectorName="San Marcos &amp; Coll. Baker" country="Nicaragua" location="Nicaragua" pageId="87" pageNumber="86" specimenCode="NVG151102-11" specimenCount="1" specimenCount-male="1" typeStatus="paratype">
and
<specimenCount id="9D2E7BA6FF88BB07C213FA3AE169B7EB" box="[537,572,1388,1417]" count="1" pageId="87" pageNumber="86" type="male">1♂</specimenCount>
NVG-5056
<collectingCountry id="F33FF0BFFF88BB07C2CCFA3AE069B7E4" box="[710,828,1388,1414]" name="Nicaragua" pageId="87" pageNumber="86">Nicaragua</collectingCountry>
:
<collectorName id="26DDD5F9FF88BB07C34CFA3AE09EB7E4" box="[838,971,1388,1414]" pageId="87" pageNumber="86">San Marcos</collectorName>
, old specimen ca. 1900,
<collectorName id="26DDD5F9FF88BB07C4D7FA3AE609B7E4" box="[1245,1372,1388,1414]" pageId="87" pageNumber="86">Coll. Baker</collectorName>
, genitalia
<specimenCode id="DB8E1854FF88BB07C0FCFADBE2F2B7CA" box="[246,423,1421,1448]" collectionCode="NVG" pageId="87" pageNumber="86">NVG151102-11</specimenCode>
[
<collectionCode id="ED3928EAFF88BB07C1BEFAD8E159B7CA" box="[436,524,1422,1448]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="87" pageNumber="86" type="Museum">USNM</collectionCode>
]
</materialsCitation>
.
</paragraph>
<paragraph id="8B97B02FFF88BB07C0CAFAEEE1A2B7B0" blockId="87.[192,759,1463,1490]" box="[192,759,1463,1490]" pageId="87" pageNumber="86">
<emphasis id="B95C6C3DFF88BB07C0CAFAEEE20EB7B3" bold="true" box="[192,347,1463,1490]" pageId="87" pageNumber="86">
<typeStatus id="54930E8DFF88BB07C0CAFAEEE3ACB7B0" box="[192,249,1464,1490]" pageId="87" pageNumber="86">Type</typeStatus>
locality.
</emphasis>
<collectingCountry id="F33FF0BFFF88BB07C16BFAE1E2E2B7B3" box="[353,439,1463,1489]" name="Mexico" pageId="87" pageNumber="86">Mexico</collectingCountry>
:
<collectingRegion id="49EC7ECDFF88BB07C1CBFAEEE17DB7B0" box="[449,552,1464,1490]" country="Mexico" name="Veracruz de Ignacio de la Llave" pageId="87" pageNumber="86">Veracruz</collectingRegion>
, Paso de San Juan.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF88BB07C0CAFAB4E744B47F" pageId="87" pageNumber="86" type="etymology">
<paragraph id="8B97B02FFF88BB07C0CAFAB4E744B47F" blockId="87.[191,1440,1505,1566]" pageId="87" pageNumber="86">
<emphasis id="B95C6C3DFF88BB07C0CAFAB4E211B79E" bold="true" box="[192,324,1506,1532]" pageId="87" pageNumber="86">Etymology.</emphasis>
The name is derived from
<taxonomicName id="4C28CBACFF88BB07C262FAB4E1B2B799" baseAuthorityName="Godman" baseAuthorityYear="1901" box="[616,743,1505,1531]" class="Insecta" family="Hesperiidae" genus="Tromba" kingdom="Animalia" order="Lepidoptera" pageId="87" pageNumber="86" phylum="Arthropoda" rank="species" species="xanthura">
<emphasis id="B95C6C3DFF88BB07C262FAB4E1B2B799" box="[616,743,1505,1531]" italics="true" pageId="87" pageNumber="86">T.xanthura</emphasis>
</taxonomicName>
, the southern counterpart of this species. It has been shortened to indicate this is a more northern species. The name is a feminine adjective.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF88BB07C0CAF97BE1C1B425" box="[192,660,1581,1608]" pageId="87" pageNumber="86" type="distribution">
<paragraph id="8B97B02FFF88BB07C0CAF97BE1C1B425" blockId="87.[192,660,1581,1608]" box="[192,660,1581,1608]" pageId="87" pageNumber="86">
<emphasis id="B95C6C3DFF88BB07C0CAF97BE20EB425" bold="true" box="[192,347,1581,1607]" pageId="87" pageNumber="86">Distribution.</emphasis>
From Mexico to
<collectingCountry id="F33FF0BFFF88BB07C211F97BE1C4B425" box="[539,657,1581,1607]" name="Nicaragua" pageId="87" pageNumber="86">Nicaragua</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>