309 lines
29 KiB
XML
309 lines
29 KiB
XML
<document ID-DOI="10.1590/S1679-62252010000200015" ID-ISSN="1982-0224" ID-Zenodo-Dep="5421615" approvalRequired="1" approvalRequired_for_textStreams="1" checkinTime="1630379760921" checkinUser="carolina" docAuthor="Blanco, Daniel Rodrigues, Lui, Roberto Laridondo, Bertollo, Luiz Antonio Carlos, Margarido, Vladimir Pavan & Filho, Orlando Moreira" docDate="2010" docId="03F187D1FFBD93515642A12CFA52F980" docLanguage="en" docName="NeotropIchthyol.2010.35.pdf" docOrigin="Neotropical Ichthyology 8 (2)" docSource="http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000200015&lng=en&tlng=en" docStyle="DocumentStyle:85A40B78B715C4497F837D9D78407CE5.4:NeotropIchthyol.2003-2013.journal_article" docStyleId="85A40B78B715C4497F837D9D78407CE5" docStyleName="NeotropIchthyol.2003-2013.journal_article" docStyleVersion="4" docTitle="Hoplias malabaricus" docType="treatment" docVersion="2" lastPageId="3" lastPageNumber="364" masterDocId="FFC8FFA9FFBC9352556BA41AFFD5FFC8" masterDocTitle="Karyotypic diversity between allopatric populations of the group Hoplias malabaricus (Characiformes: Erythrinidae): evolutionary and biogeographic considerations" masterLastPageNumber="368" masterPageNumber="361" pageId="1" pageNumber="362" updateTime="1630687255722" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0" zenodo-license-figures="CC-BY-4.0">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Karyotypic diversity between allopatric populations of the group Hoplias malabaricus (Characiformes: Erythrinidae): evolutionary and biogeographic considerations</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Blanco, Daniel Rodrigues</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Lui, Roberto Laridondo</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Bertollo, Luiz Antonio Carlos</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Margarido, Vladimir Pavan</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Filho, Orlando Moreira</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>Neotropical Ichthyology</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2010</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2010-12-31</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>8</mods:number>
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</mods:detail>
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<mods:detail type="issue">
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<mods:number>2</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>361</mods:start>
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<mods:end>368</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000200015&lng=en&tlng=en</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">10.1590/S1679-62252010000200015</mods:identifier>
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<mods:identifier type="ISSN">1982-0224</mods:identifier>
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<mods:identifier type="Zenodo-Dep">5421615</mods:identifier>
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</mods:mods>
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<treatment LSID="urn:lsid:plazi:treatment:03F187D1FFBD93515642A12CFA52F980" httpUri="http://treatment.plazi.org/id/03F187D1FFBD93515642A12CFA52F980" lastPageId="3" lastPageNumber="364" pageId="1" pageNumber="362">
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<subSubSection lastPageId="2" lastPageNumber="363" pageId="1" pageNumber="362" type="nomenclature">
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<paragraph blockId="1.[809,1452,1334,1961]" box="[809,1435,1334,1353]" pageId="1" pageNumber="362">
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<heading box="[809,1435,1334,1353]" centered="true" fontSize="11" level="2" pageId="1" pageNumber="362" reason="3">
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<emphasis bold="true" box="[809,1435,1334,1353]" pageId="1" pageNumber="362">
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<taxonomicName baseAuthorityName="Bloch" baseAuthorityYear="1794" box="[809,1036,1334,1353]" class="Actinopterygii" family="Erythrinidae" genus="Hoplias" kingdom="Animalia" order="Characiformes" pageId="1" pageNumber="362" phylum="Chordata" rank="species" species="malabaricus">
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<emphasis bold="true" box="[809,1036,1334,1353]" italics="true" pageId="1" pageNumber="362">Hoplias malabaricus</emphasis>
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</taxonomicName>
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from the São Francisco River basin
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</emphasis>
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</heading>
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</paragraph>
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<paragraph blockId="1.[809,1452,1334,1961]" lastBlockId="2.[136,779,182,265]" lastPageId="2" lastPageNumber="363" pageId="1" pageNumber="362">
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<materialsCitation collectionCode="FN, FISH" collectorName="Fundamental Number & Submetacentric & Fluorescent & S Hind" elevation="22" lastPageId="2" lastPageNumber="363" latitude="12.13" pageId="1" pageNumber="362" specimenCount="1">
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The specimens from this population had 2n = 42 chromosomes (
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<quantity box="[964,1016,1398,1417]" metricMagnitude="1" metricUnit="m" metricValue="2.2" pageId="1" pageNumber="362" unit="m" value="22.0">
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<elevation box="[964,1016,1398,1417]" metricMagnitude="1" metricUnit="m" metricValue="2.2" pageId="1" pageNumber="362" unit="m" value="22.0">22 m</elevation>
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</quantity>
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+ 20 sm) and
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<collectorName box="[1154,1378,1398,1417]" pageId="1" pageNumber="362">Fundamental Number</collectorName>
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(
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<collectionCode box="[1389,1425,1398,1417]" pageId="1" pageNumber="362">FN</collectionCode>
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) =
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<quantity box="[809,872,1430,1449]" metricMagnitude="0" metricUnit="m" metricValue="2.1336" pageId="1" pageNumber="362" unit="in" value="84.0">84 in</quantity>
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both males and females, with no chromosome heteromorphism related to sex. C-banding revealed pericentromeric bands in the majority of chromosomes; some chromosomes also exhibited terminal and interstitial bands.
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<collectorName box="[809,973,1558,1577]" pageId="1" pageNumber="362">Submetacentric</collectorName>
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pair 17 clearly stood out, with a large band in the terminal region of the long arm, which were co-localized with the Ag-NORs (
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<figureCitation box="[1051,1203,1622,1641]" captionStart="Fig" captionStartId="2.[136,175,1877,1896]" captionTargetBox="[223,1343,636,1821]" captionTargetId="figure-276@2.[204,1376,614,1845]" captionTargetPageId="2" captionText="Fig. 1. Hoplias malabaricus karyotypes (karyomorph A) with conventional Giemsa staining (a, d, g) and C-banding (b, e, h) of the populations from the basins of the São Francisco (a, b), Araguaia (d, e) and Xingu (g, h) Rivers. Boxes display chromosomes with Ag-NORs in the populations from the São Francisco (c), Araguaia (f) and Xingu (i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421617" httpUri="https://zenodo.org/record/5421617/files/figure.png" pageId="1" pageNumber="362">Fig. 1a, b, c</figureCitation>
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).
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<collectorName box="[1230,1364,1622,1641]" pageId="1" pageNumber="362">Fluorescent</collectorName>
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<emphasis box="[1376,1452,1622,1641]" italics="true" pageId="1" pageNumber="362">in situ</emphasis>
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hybridization (
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<collectionCode box="[965,1025,1654,1673]" pageId="1" pageNumber="362">FISH</collectionCode>
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) with the 5S rDNA probe revealed two chromosome pairs with interstitial sites: one small metacentric pair (n 10) with sites in the long arm and one large submetacentric pair (n 13) with sites in the short arm near the centromere (
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<figureCitation box="[948,1031,1782,1801]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="1" pageNumber="362">Fig. 2a</figureCitation>
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). 18S rDNA sites were found in four chromosome pairs: two metacentric pairs (n 6 and 9) with sites located in the terminal region of both chromosome arms and two submetacentric pairs (n 16 and 17) with sites in the long arms in the region near the centromere and the terminal region, respectively (
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<figureCitation box="[949,1026,1942,1961]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="1" pageNumber="362">Fig. 2b</figureCitation>
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).
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<collectionCode box="[1044,1101,1942,1961]" pageId="1" pageNumber="362">FISH</collectionCode>
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with the 5
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<collectorName box="[1208,1276,1942,1961]" pageId="1" pageNumber="362">
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S
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<emphasis box="[1223,1276,1942,1961]" italics="true" pageId="1" pageNumber="362">Hind</emphasis>
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</collectorName>
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III satellite DNA probe identified nine chromosome pairs with sites in the centromeric region: three metacentric pairs (
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<geoCoordinate box="[580,635,214,233]" degrees="1, 5" direction="north" orientation="latitude" pageId="2" pageNumber="363" precision="5555" value="1.5">n 1, 5</geoCoordinate>
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and 6) and six submetacentric pairs (
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<geoCoordinate box="[360,443,246,265]" degrees="12, 13" direction="north" orientation="latitude" pageId="2" pageNumber="363" precision="555" value="12.13">n 12, 13</geoCoordinate>
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, 14, 18, 19 and 21) (
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<figureCitation box="[646,720,246,265]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="2" pageNumber="363">Fig. 2c</figureCitation>
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)
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<subSubSection lastPageId="3" lastPageNumber="364" pageId="2" pageNumber="363" type="description">
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<paragraph blockId="2.[136,779,310,585]" box="[136,708,310,329]" pageId="2" pageNumber="363">
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<emphasis bold="true" box="[136,708,310,329]" pageId="2" pageNumber="363">
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<taxonomicName baseAuthorityName="Bloch" baseAuthorityYear="1794" box="[136,363,310,329]" class="Actinopterygii" family="Erythrinidae" genus="Hoplias" kingdom="Animalia" order="Characiformes" pageId="2" pageNumber="363" phylum="Chordata" rank="species" species="malabaricus">
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<emphasis bold="true" box="[136,363,310,329]" italics="true" pageId="2" pageNumber="363">Hoplias malabaricus</emphasis>
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</taxonomicName>
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from the Araguaia River basin
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</emphasis>
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</paragraph>
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<paragraph blockId="2.[136,779,310,585]" lastBlockId="2.[816,1460,182,585]" pageId="2" pageNumber="363">
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<materialsCitation collectionCode="FISH" collectorName="Moreover & S Hind" elevation="18" latitude="10.11" location="The" municipality="Sao Miguel do Araguaia" pageId="2" pageNumber="363" specimenCount="1">
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<location LSID="urn:lsid:plazi:treatment:03F187D1FFBD93515642A12CFA52F980:8E87601CFFBE935055C5A54CFF0DFEA1" box="[174,216,342,361]" latitude="10.11" municipality="Sao Miguel do Araguaia" name="The" pageId="2" pageNumber="363">The</location>
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specimens collected in
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<collectingMunicipality box="[472,734,342,361]" pageId="2" pageNumber="363">São Miguel do Araguaia</collectingMunicipality>
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had 2n = 42 chromosomes (
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<quantity box="[393,449,374,393]" metricMagnitude="1" metricUnit="m" metricValue="1.8" pageId="2" pageNumber="363" unit="m" value="18.0">
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<elevation box="[393,449,374,393]" metricMagnitude="1" metricUnit="m" metricValue="1.8" pageId="2" pageNumber="363" unit="m" value="18.0">18 m</elevation>
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</quantity>
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+ 24 sm) and FN =
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<quantity box="[668,723,374,393]" metricMagnitude="0" metricUnit="m" metricValue="2.1336" pageId="2" pageNumber="363" unit="in" value="84.0">84 in</quantity>
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both males and females, with no chromosome heteromorphism related to sex. Heterochromatin was found distributed in the pericentromeric and terminal region in several chromosomes of the complement. Submetacentric pair 10 clearly stood out, with a large band in the pericentromeric region; pairs 14 and 20 had conspicuous bands in the terminal region of the long arm, which were co-localized with the Ag-NORs (
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<figureCitation box="[980,1110,214,233]" captionStart="Fig" captionStartId="2.[136,175,1877,1896]" captionTargetBox="[223,1343,636,1821]" captionTargetId="figure-276@2.[204,1376,614,1845]" captionTargetPageId="2" captionText="Fig. 1. Hoplias malabaricus karyotypes (karyomorph A) with conventional Giemsa staining (a, d, g) and C-banding (b, e, h) of the populations from the basins of the São Francisco (a, b), Araguaia (d, e) and Xingu (g, h) Rivers. Boxes display chromosomes with Ag-NORs in the populations from the São Francisco (c), Araguaia (f) and Xingu (i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421617" httpUri="https://zenodo.org/record/5421617/files/figure.png" pageId="2" pageNumber="363">Fig. 1d, e, f</figureCitation>
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).
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<collectionCode box="[1128,1187,214,233]" pageId="2" pageNumber="363">FISH</collectionCode>
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with the 5S rDNA probe revealed only one small metacentric pair (n 8) labeled in the interstitial region of the long arms (
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<figureCitation box="[1193,1273,278,297]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="2" pageNumber="363">Fig. 2d</figureCitation>
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). 18S rDNA sites were labeled in three pairs of submetacentric chromosomes: one large pair (n 10) in the region near the centromere and two smaller pairs (n 14 and 20) in the terminal region of the long arms.
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<collectorName box="[935,1044,406,425]" pageId="2" pageNumber="363">Moreover</collectorName>
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, only one of the homologues of pair 10 had an extra site, which was in the terminal region of the long arm (
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<figureCitation box="[929,1009,470,489]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="2" pageNumber="363">Fig. 2e</figureCitation>
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).
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<collectionCode box="[1028,1087,470,489]" pageId="2" pageNumber="363">FISH</collectionCode>
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with the 5
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<collectorName box="[1203,1273,470,489]" pageId="2" pageNumber="363">
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S
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<emphasis box="[1218,1273,470,489]" italics="true" pageId="2" pageNumber="363">Hind</emphasis>
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</collectorName>
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III satellite DNA probe identified eight chromosome pairs with sites in the centromeric region: two metacentric pairs (n 1 and 5) and six submetacentic pairs (
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<geoCoordinate box="[1043,1131,566,585]" degrees="10, 11" direction="north" orientation="latitude" pageId="2" pageNumber="363" precision="555" value="10.11">n 10, 11</geoCoordinate>
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, 12, 15, 16 and 21) (
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<figureCitation box="[1345,1421,566,585]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="2" pageNumber="363">Fig. 2f</figureCitation>
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)
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</materialsCitation>
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.
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</paragraph>
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<caption ID-DOI="http://doi.org/10.5281/zenodo.5421617" ID-Zenodo-Dep="5421617" httpUri="https://zenodo.org/record/5421617/files/figure.png" pageId="2" pageNumber="363" startId="2.[136,175,1877,1896]" targetBox="[223,1343,636,1821]" targetPageId="2">
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<paragraph blockId="2.[136,1459,1877,1960]" pageId="2" pageNumber="363">
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<emphasis bold="true" box="[136,204,1877,1896]" pageId="2" pageNumber="363">Fig. 1.</emphasis>
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<taxonomicName baseAuthorityName="Bloch" baseAuthorityYear="1794" box="[209,432,1877,1896]" class="Actinopterygii" family="Erythrinidae" genus="Hoplias" kingdom="Animalia" order="Characiformes" pageId="2" pageNumber="363" phylum="Chordata" rank="species" species="malabaricus">
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<emphasis box="[209,432,1877,1896]" italics="true" pageId="2" pageNumber="363">Hoplias malabaricus</emphasis>
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</taxonomicName>
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karyotypes (karyomorph A) with conventional Giemsa staining (
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<emphasis bold="true" box="[1111,1124,1877,1896]" pageId="2" pageNumber="363">a</emphasis>
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,
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<emphasis bold="true" box="[1136,1151,1877,1896]" pageId="2" pageNumber="363">d</emphasis>
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,
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<emphasis bold="true" box="[1162,1175,1877,1896]" pageId="2" pageNumber="363">g</emphasis>
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) and C-banding (
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<emphasis bold="true" box="[1359,1374,1877,1896]" pageId="2" pageNumber="363">b</emphasis>
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,
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<emphasis bold="true" box="[1385,1397,1877,1896]" pageId="2" pageNumber="363">e</emphasis>
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,
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<emphasis bold="true" box="[1408,1423,1877,1896]" pageId="2" pageNumber="363">h</emphasis>
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) of the populations from the basins of the São Francisco (
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<emphasis bold="true" box="[688,701,1909,1928]" pageId="2" pageNumber="363">a</emphasis>
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,
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<emphasis bold="true" box="[712,727,1909,1928]" pageId="2" pageNumber="363">b</emphasis>
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), Araguaia (
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<emphasis bold="true" box="[856,870,1909,1928]" pageId="2" pageNumber="363">d</emphasis>
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,
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<emphasis bold="true" box="[881,893,1909,1928]" pageId="2" pageNumber="363">e</emphasis>
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) and Xingu (
|
|
<emphasis bold="true" box="[1029,1042,1909,1928]" pageId="2" pageNumber="363">g</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[1053,1068,1909,1928]" pageId="2" pageNumber="363">h</emphasis>
|
|
) Rivers. Boxes display chromosomes with Ag-NORs in the populations from the São Francisco (
|
|
<emphasis bold="true" box="[758,770,1941,1960]" pageId="2" pageNumber="363">c</emphasis>
|
|
), Araguaia (
|
|
<emphasis bold="true" box="[903,912,1941,1960]" pageId="2" pageNumber="363">f</emphasis>
|
|
) and Xingu (
|
|
<emphasis bold="true" box="[1052,1059,1941,1960]" pageId="2" pageNumber="363">i</emphasis>
|
|
) Rivers. Scale bar = 5μm.
|
|
</paragraph>
|
|
</caption>
|
|
<caption ID-DOI="http://doi.org/10.5281/zenodo.5421619" ID-Zenodo-Dep="5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="3" pageNumber="364" startId="3.[129,168,1387,1406]" targetBox="[129,1452,174,1370]" targetPageId="3">
|
|
<paragraph blockId="3.[129,1451,1387,1470]" pageId="3" pageNumber="364">
|
|
<emphasis bold="true" box="[129,197,1387,1406]" pageId="3" pageNumber="364">Fig. 2.</emphasis>
|
|
<taxonomicName baseAuthorityName="Bloch" baseAuthorityYear="1794" box="[202,424,1387,1406]" class="Actinopterygii" family="Erythrinidae" genus="Hoplias" kingdom="Animalia" order="Characiformes" pageId="3" pageNumber="364" phylum="Chordata" rank="species" species="malabaricus">
|
|
<emphasis box="[202,424,1387,1406]" italics="true" pageId="3" pageNumber="364">Hoplias malabaricus</emphasis>
|
|
</taxonomicName>
|
|
karyotypes (karyomorph A) with fluorescent
|
|
<emphasis box="[901,965,1387,1406]" italics="true" pageId="3" pageNumber="364">in situ</emphasis>
|
|
hybridization (FISH) using 5S rDNA (
|
|
<emphasis bold="true" box="[1372,1385,1387,1406]" pageId="3" pageNumber="364">a</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[1396,1411,1387,1406]" pageId="3" pageNumber="364">d</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[1423,1436,1387,1406]" pageId="3" pageNumber="364">g</emphasis>
|
|
), 18S rDNA (
|
|
<emphasis bold="true" box="[255,270,1419,1438]" pageId="3" pageNumber="364">b</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[281,293,1419,1438]" pageId="3" pageNumber="364">e</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[305,320,1419,1438]" pageId="3" pageNumber="364">h</emphasis>
|
|
) and 5S
|
|
<emphasis box="[405,458,1419,1438]" italics="true" pageId="3" pageNumber="364">Hind</emphasis>
|
|
III satellite DNA (
|
|
<emphasis bold="true" box="[648,660,1419,1438]" pageId="3" pageNumber="364">c</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[672,681,1419,1438]" pageId="3" pageNumber="364">f</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[692,699,1419,1438]" pageId="3" pageNumber="364">i</emphasis>
|
|
) probes in the populations from the basins of the São Francisco (
|
|
<emphasis bold="true" box="[1373,1386,1419,1438]" pageId="3" pageNumber="364">a</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[1398,1413,1419,1438]" pageId="3" pageNumber="364">b</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[1424,1436,1419,1438]" pageId="3" pageNumber="364">c</emphasis>
|
|
), Araguaia (
|
|
<emphasis bold="true" box="[241,256,1451,1470]" pageId="3" pageNumber="364">d</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[267,279,1451,1470]" pageId="3" pageNumber="364">e</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[291,300,1451,1470]" pageId="3" pageNumber="364">f</emphasis>
|
|
) and Xingu (
|
|
<emphasis bold="true" box="[438,451,1451,1470]" pageId="3" pageNumber="364">g</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[463,478,1451,1470]" pageId="3" pageNumber="364">h</emphasis>
|
|
,
|
|
<emphasis bold="true" box="[490,497,1451,1470]" pageId="3" pageNumber="364">i</emphasis>
|
|
) Rivers. Scale bar = 5μm.
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph blockId="3.[129,772,1525,1960]" box="[129,735,1525,1544]" pageId="3" pageNumber="364">
|
|
<emphasis bold="true" box="[129,735,1525,1544]" pageId="3" pageNumber="364">
|
|
<taxonomicName baseAuthorityName="Bloch" baseAuthorityYear="1794" box="[129,356,1525,1544]" class="Actinopterygii" family="Erythrinidae" genus="Hoplias" kingdom="Animalia" order="Characiformes" pageId="3" pageNumber="364" phylum="Chordata" rank="species" species="malabaricus">
|
|
<emphasis bold="true" box="[129,356,1525,1544]" italics="true" pageId="3" pageNumber="364">Hoplias malabaricus</emphasis>
|
|
</taxonomicName>
|
|
from the basin of the Xingu River
|
|
</emphasis>
|
|
</paragraph>
|
|
<paragraph blockId="3.[129,772,1525,1960]" lastBlockId="3.[809,1452,1525,1608]" pageId="3" pageNumber="364">
|
|
<materialsCitation collectionCode="FISH" collectorName="S Hind" county="Silver" elevation="20" latitude="11.12" location="The" municipality="Sete de Setembro River" pageId="3" pageNumber="364" specimenCount="1">
|
|
<location LSID="urn:lsid:plazi:treatment:03F187D1FFBD93515642A12CFA52F980:8E87601CFFBF935155CDA20FFF05F9E0" box="[166,208,1557,1576]" county="Silver" latitude="11.12" municipality="Sete de Setembro River" name="The" pageId="3" pageNumber="364">The</location>
|
|
specimens collected from the tributaries of the
|
|
<collectingMunicipality pageId="3" pageNumber="364">Sete de Setembro River</collectingMunicipality>
|
|
had 2n = 42 chromosomes (
|
|
<quantity box="[620,674,1589,1608]" metricMagnitude="1" metricUnit="m" metricValue="2.0" pageId="3" pageNumber="364" unit="m" value="20.0">
|
|
<elevation box="[620,674,1589,1608]" metricMagnitude="1" metricUnit="m" metricValue="2.0" pageId="3" pageNumber="364" unit="m" value="20.0">20 m</elevation>
|
|
</quantity>
|
|
+ 22 sm) and FN = 84 for both males and females, with no heteromorphic sex chromosome system. C-banding revealed pericentromeric bands in nearly all the chromosomes as well as small terminal bands on some pairs.
|
|
<collectingCounty box="[371,435,1717,1736]" pageId="3" pageNumber="364">Silver</collectingCounty>
|
|
nitrate staining revealed three chromosome pairs (
|
|
<geoCoordinate box="[331,387,1749,1768]" degrees="4, 9" direction="north" orientation="latitude" pageId="3" pageNumber="364" precision="5555" value="4.9">n 4, 9</geoCoordinate>
|
|
and 15) withAg-NORs (
|
|
<figureCitation box="[641,758,1749,1768]" captionStart="Fig" captionStartId="2.[136,175,1877,1896]" captionTargetBox="[223,1343,636,1821]" captionTargetId="figure-276@2.[204,1376,614,1845]" captionTargetPageId="2" captionText="Fig. 1. Hoplias malabaricus karyotypes (karyomorph A) with conventional Giemsa staining (a, d, g) and C-banding (b, e, h) of the populations from the basins of the São Francisco (a, b), Araguaia (d, e) and Xingu (g, h) Rivers. Boxes display chromosomes with Ag-NORs in the populations from the São Francisco (c), Araguaia (f) and Xingu (i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421617" httpUri="https://zenodo.org/record/5421617/files/figure.png" pageId="3" pageNumber="364">
|
|
Fig.
|
|
<quantity box="[687,715,1749,1768]" metricMagnitude="-3" metricUnit="kg" metricValue="1.0" pageId="3" pageNumber="364" unit="g" value="1.0">1g</quantity>
|
|
, h, i
|
|
</figureCitation>
|
|
).
|
|
<collectionCode box="[129,187,1781,1800]" pageId="3" pageNumber="364">FISH</collectionCode>
|
|
with the 5S rDNA probe revealed only one metacentric pair (n 8) labeled in the interstitial region of the long arms (
|
|
<figureCitation box="[137,215,1845,1864]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="3" pageNumber="364">
|
|
Fig.
|
|
<quantity box="[185,215,1845,1864]" metricMagnitude="-3" metricUnit="kg" metricValue="2.0" pageId="3" pageNumber="364" unit="g" value="2.0">2g</quantity>
|
|
</figureCitation>
|
|
). 18S rDNA sites were labeled in the terminal region of the long arms in three pairs of chromosomes: two metacentric pairs (n 4 and 9) and one submetacentric pair (n 15) (
|
|
<figureCitation box="[179,260,1941,1960]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="3" pageNumber="364">Fig. 2h</figureCitation>
|
|
).
|
|
<collectionCode box="[279,337,1941,1960]" pageId="3" pageNumber="364">FISH</collectionCode>
|
|
with the 5
|
|
<collectorName box="[451,520,1941,1960]" pageId="3" pageNumber="364">
|
|
S
|
|
<emphasis box="[466,520,1941,1960]" italics="true" pageId="3" pageNumber="364">Hind</emphasis>
|
|
</collectorName>
|
|
III satellite DNA probe identified 10 chromosome pairs with sites in the centromeric region: four metacentric pairs (
|
|
<geoCoordinate box="[1163,1234,1557,1576]" degrees="1, 5" direction="north" orientation="latitude" pageId="3" pageNumber="364" precision="5555" value="1.5">n 1, 5</geoCoordinate>
|
|
, 7 and 10) and six submetacentric pairs (
|
|
<geoCoordinate box="[1038,1124,1589,1608]" degrees="11, 12" direction="north" orientation="latitude" pageId="3" pageNumber="364" precision="555" value="11.12">n 11, 12</geoCoordinate>
|
|
, 13, 16, 17 and 19) (
|
|
<figureCitation box="[1331,1402,1589,1608]" captionStart="Fig" captionStartId="3.[129,168,1387,1406]" captionTargetBox="[129,1452,174,1370]" captionTargetId="figure-352@3.[129,1452,174,1370]" captionTargetPageId="3" captionText="Fig. 2. Hoplias malabaricus karyotypes (karyomorph A) with fluorescent in situ hybridization (FISH) using 5S rDNA (a, d, g), 18S rDNA (b, e, h) and 5SHindIII satellite DNA (c, f, i) probes in the populations from the basins of the São Francisco (a, b, c), Araguaia (d, e, f) and Xingu (g, h, i) Rivers. Scale bar = 5μm." figureDoi="http://doi.org/10.5281/zenodo.5421619" httpUri="https://zenodo.org/record/5421619/files/figure.png" pageId="3" pageNumber="364">Fig. 2i</figureCitation>
|
|
)
|
|
</materialsCitation>
|
|
.
|
|
</paragraph>
|
|
</subSubSection>
|
|
</treatment>
|
|
</document> |