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<document id="27FC108C8DBB7CAB29A35C8B59F53207" ID-DOI="10.11646/phytotaxa.642.2.2" ID-ISSN="1179-3163" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatmentCitations_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722602361337" checkinUser="felipe" docAuthor="Yang, Yan-Yan, Thiyagaraja, Vinodhini, Wanasinghe, Dhanushka N., Ar, Gomes De Farias &amp; Zhao, Qi" docDate="2024" docId="039F879EFFEDFFB231F51BD6FED4FEA9" docLanguage="en" docName="phytotaxa.642.2.2.pdf" docOrigin="Phytotaxa 642 (2)" docSource="http://dx.doi.org/10.11646/phytotaxa.642.2.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Acanthostigmina multiseptatum Boonmee &amp; K. D. Hyde" docType="treatment" docVersion="1" lastPageNumber="134" masterDocId="FFA6FFE6FFEAFFBA317D1F58FFC4FFA9" masterDocTitle="Phylogenetic and taxonomic appraisal of Neomanoharachariella xizangensis sp. nov. and the first asexual report of Acanthostigmina (Tubeufiaceae, Tubeufiales) from Xizang Autonomous Region, China" masterLastPageNumber="140" masterPageNumber="127" pageNumber="134" updateTime="1722801766765" updateUser="felipe" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="C4A49113DC80FF65CB8E60394FB85D33">Phylogenetic and taxonomic appraisal of Neomanoharachariella xizangensis sp. nov. and the first asexual report of Acanthostigmina (Tubeufiaceae, Tubeufiales) from Xizang Autonomous Region, China</mods:title>
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<mods:namePart id="C90766338BAA5CCEA5462E9EC00CAFF7">Yang, Yan-Yan</mods:namePart>
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<mods:affiliation id="26BF992C90E634EA8D232DC13262F400">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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<mods:namePart id="71AA84A8DEF8288D1151FF73C2EC7FEF">Thiyagaraja, Vinodhini</mods:namePart>
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<mods:affiliation id="F082015217EA283EB0A916098B29015C">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China</mods:affiliation>
<mods:nameIdentifier id="A652F6CAFD70EBD7468D462A7433A040" type="email">vino.thiyagaraja@gmail.com</mods:nameIdentifier>
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<mods:namePart id="E2878821BF6F08CCF8F0802E13B4165B">Wanasinghe, Dhanushka N.</mods:namePart>
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<mods:affiliation id="8CC0CAE28AFE34E36A32BCAEDD88F50E">Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County 654400, Yunnan Province, P. R. China</mods:affiliation>
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<mods:namePart id="68FAE109BEF9E902C341A7AECC74C596">Ar, Gomes De Farias</mods:namePart>
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<mods:affiliation id="809DC4C7448EA739493D72AB32DBDAF4">Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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<mods:namePart id="1ACE8825C3A61514033CEB7B18EF08BC">Zhao, Qi</mods:namePart>
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<mods:affiliation id="2863A866A9A77202E02521E823F71FA8">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China</mods:affiliation>
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<mods:title id="6AF34CF929F4BBC3679E4D8C8A635F7D">Phytotaxa</mods:title>
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<mods:date id="97C170D27676914386F9E1A62F5D4588">2024</mods:date>
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<mods:number id="660824CD0BBAD47BBDE6E2AEF5709AEB">642</mods:number>
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<mods:identifier id="D637C1BD768C3CC580ACF427DFA7D082" type="DOI">10.11646/phytotaxa.642.2.2</mods:identifier>
<mods:identifier id="3A233A42322B045DE146A13FB4823D4E" type="ISSN">1179-3163</mods:identifier>
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<treatment id="039F879EFFEDFFB231F51BD6FED4FEA9" LSID="urn:lsid:plazi:treatment:039F879EFFEDFFB231F51BD6FED4FEA9" httpUri="http://treatment.plazi.org/id/039F879EFFEDFFB231F51BD6FED4FEA9" lastPageId="8" lastPageNumber="134" pageId="7" pageNumber="134">
<subSubSection id="C32C6503FFEDFFBD31F51BD6FC6CFB64" pageId="7" pageNumber="134" type="nomenclature">
<paragraph id="8B893688FFEDFFBD31F51BD6FBE4FB01" blockId="7.[136,1056,1166,1229]" box="[136,1056,1166,1193]" pageId="7" pageNumber="134">
<heading id="D0C181E4FFEDFFBD31F51BD6FBE4FB01" bold="true" box="[136,1056,1166,1193]" fontSize="11" level="1" pageId="7" pageNumber="134" reason="1">
<taxonomicName id="4C364D0BFFEDFFBD31F51BD6FBE4FB01" ID-CoL="927Q" authority="(Promp. &amp; A. N. Mill.) Boonmee &amp; K. D. Hyde" authorityName="Boonmee &amp; K. D. Hyde" baseAuthorityName="Promp. &amp; A. N. Mill." box="[136,1056,1166,1193]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD31F51BD6FBE4FB01" bold="true" box="[136,1056,1166,1193]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31F51BD6FE3EFB00" bold="true" box="[136,506,1166,1193]" italics="true" pageId="7" pageNumber="134">Acanthostigmina multiseptatum</emphasis>
(Promp. &amp; A.N. Mill.) Boonmee &amp; K.D. Hyde
</emphasis>
</taxonomicName>
</heading>
</paragraph>
<paragraph id="8B893688FFEDFFBD31F51BEAFC6CFB64" blockId="7.[136,1056,1166,1229]" box="[136,936,1202,1229]" pageId="7" pageNumber="134">Index Fungorum number: IF 550681, Facesoffungi number: FoF 15235</paragraph>
</subSubSection>
<subSubSection id="C32C6503FFEDFFBD31F51BA2FEF2F9D5" pageId="7" pageNumber="134" type="reference_group">
<paragraph id="8B893688FFEDFFBD31F51BA2FB6DFABD" blockId="7.[136,1452,1274,2093]" box="[136,1193,1274,1301]" pageId="7" pageNumber="134">
Basionym:
<treatmentCitation id="0A971099FFEDFFBD30751BA2FB6DFABD" author="Promp. &amp; A. N. Mill." box="[264,1193,1274,1301]" issue="3" journal="Mycologia" page="577" pageId="7" pageNumber="134" score="9.729594737742866" volume="102" year="2010">
<taxonomicName id="4C364D0BFFEDFFBD30751BA2FC09FABD" ID-CoL="926G" authority="Promp. &amp; A. N. Mill., Mycologia" authorityName="Promp. &amp; A. N. Mill., Mycologia" box="[264,973,1274,1301]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigma" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD30751BA2FD92FABD" box="[264,598,1274,1300]" italics="true" pageId="7" pageNumber="134">Acanthostigma multiseptatum</emphasis>
Promp. &amp; A.N. Mill., Mycologia
</taxonomicName>
102(3): 577 (2010)
</treatmentCitation>
</paragraph>
<paragraph id="8B893688FFEDFFBD31C01A46FEF2F9D5" blockId="7.[136,1452,1274,2093]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01A46FEE5FA91" box="[189,289,1310,1336]" italics="true" pageId="7" pageNumber="134">Saprobic</emphasis>
on the dead wood of an unidentified plant.
<emphasis id="B942EA9AFFEDFFBD338F1A46FC64FA91" bold="true" box="[754,928,1310,1336]" pageId="7" pageNumber="134">Sexual morph:</emphasis>
unidentified.
<emphasis id="B942EA9AFFEDFFBD354A1A46FB30FA91" bold="true" box="[1079,1268,1310,1336]" pageId="7" pageNumber="134">Asexual morph:</emphasis>
<emphasis id="B942EA9AFFEDFFBD35841A46FA68FA91" box="[1273,1452,1310,1336]" italics="true" pageId="7" pageNumber="134">Hyphomycetous</emphasis>
helicosporous.
<emphasis id="B942EA9AFFEDFFBD304D1A1AFE50FAF5" box="[304,404,1346,1372]" italics="true" pageId="7" pageNumber="134">Colonies</emphasis>
on the substratum superficial, effuse, gregarious, pale pink.
<emphasis id="B942EA9AFFEDFFBD35511A1AFB5EFAF5" box="[1068,1178,1346,1372]" italics="true" pageId="7" pageNumber="134">Mycelium</emphasis>
partly superficial, partly immersed, hyaline, septate, branched hyphae, with crowded masses.
<emphasis id="B942EA9AFFEDFFBD32EE1A3EFBFCFA29" box="[915,1080,1382,1408]" italics="true" pageId="7" pageNumber="134">Conidiophores</emphasis>
137166 µm × 2.43.7 µm (x̄ = 152 × 3 µm, n = 5), 12 µm wide at the apex, macronematous, mononematous, erect, straight, cylindrical, hyaline, unbranched, septate, smooth-walled.
<emphasis id="B942EA9AFFEDFFBD33501AF6FCD6FA61" box="[557,786,1454,1480]" italics="true" pageId="7" pageNumber="134">Conidiogenous cells</emphasis>
812 µm long, 34.3 µm wide (x̄ = 9.5 × 3.6 µm, n = 6), holoblastic, monoblastic, integrated, cylindrical, arising laterally from the conidiophores, smooth-walled.
<emphasis id="B942EA9AFFEDFFBD342C1A8AFA6FFA45" box="[1361,1451,1490,1516]" italics="true" pageId="7" pageNumber="134">Conidia</emphasis>
166219 µm × 5.78.9 µm in the broadest part (x̄ = 203 × 7 µm, n = 25), solitary, pleurogenous, helicoid, 3040- septate, slightly constricted at the septa, tightly coiled 2 to 3 times, not becoming loose in water, rounded at the tip, 3443 µm diam, tapering to 1.33.2 µm wide near the apex, 2.74 µm wide at the base, hyaline, shiny, guttulate, smooth-walled.
</paragraph>
</subSubSection>
<subSubSection id="C32C6503FFEDFFB231C019DEFED4FEA9" lastPageId="8" lastPageNumber="135" pageId="7" pageNumber="134" type="description">
<paragraph id="8B893688FFEDFFBD31C019DEFB95F941" blockId="7.[136,1452,1274,2093]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C019DEFE7AF909" box="[189,446,1670,1696]" italics="true" pageId="7" pageNumber="134">Culture characteristics</emphasis>
:
<emphasis id="B942EA9AFFEDFFBD30B119DEFDE2F909" box="[460,550,1670,1696]" italics="true" pageId="7" pageNumber="134">Conidia</emphasis>
germinating on PDA and germ tubes produced from conidia within 8 h.
<emphasis id="B942EA9AFFEDFFBD343519DEFA68F909" box="[1352,1452,1670,1696]" italics="true" pageId="7" pageNumber="134">Colonies</emphasis>
growing on PDA, oval, edge irregular, raised, slightly sparse, reaching
<quantity id="4CCE9B6DFFEDFFBD32D519F2FC2EF96C" box="[936,1002,1706,1733]" metricMagnitude="-3" metricUnit="m" metricValue="5.0" pageId="7" pageNumber="134" unit="mm" value="5.0">5 mm</quantity>
in 1 week at 18 ℃, yellowish-brown in PDA medium, mycelium superficial and partially immersed, reverse yellowish-brown.
</paragraph>
<paragraph id="8B893688FFEDFFBD31C019AAFF12F8FC" blockId="7.[136,1452,1274,2093]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C019AAFE55F8A5" box="[189,401,1778,1804]" italics="true" pageId="7" pageNumber="134">Material examined</emphasis>
:
<collectingCountry id="F3217618FFEDFFBD30E219AAFE21F8A5" box="[415,485,1778,1804]" name="China" pageId="7" pageNumber="134">China</collectingCountry>
,
<collectingRegion id="49F2F86AFFEDFFBD309219AAFD84F8A5" box="[495,576,1778,1804]" country="China" name="Xizang" pageId="7" pageNumber="134">Xizang</collectingRegion>
Autonomous Region, Shigatse City, Nyalam County,
<geoCoordinate id="EE02504FFFEDFFBD35EB19AAFAEEF8A4" box="[1174,1322,1778,1805]" degrees="85.982237" direction="north" orientation="latitude" pageId="7" pageNumber="134" precision="1" value="85.98224">85.982237 N</geoCoordinate>
,
<geoCoordinate id="EE02504FFFEDFFBD344919AAFF5DF898" degrees="28.155186" direction="east" orientation="longitude" pageId="7" pageNumber="134" precision="1" value="28.155186">28.155186 E</geoCoordinate>
, on an unidentified dead wood.
<date id="FF881048FFEDFFBD3376184EFD57F899" box="[523,659,1814,1840]" pageId="7" pageNumber="134" value="2022-07-06">6 July 2022</date>
, Yanyan Yang, YYY394 (HKAS 131155), living culture KUNCC 23- 15803.
</paragraph>
<paragraph id="8B893688FFEDFFB231C01807FED4FEA9" blockId="7.[136,1452,1274,2093]" lastBlockId="8.[136,1451,158,256]" lastPageId="8" lastPageNumber="135" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01807FF39F8D1" box="[189,253,1887,1912]" italics="true" pageId="7" pageNumber="134">Notes</emphasis>
: In the combined phylogenetic analyses (LSU, ITS,
<emphasis id="B942EA9AFFEDFFBD32F51806FC1DF8D1" box="[904,985,1886,1912]" italics="true" pageId="7" pageNumber="134">tef 1-α</emphasis>
, and
<emphasis id="B942EA9AFFEDFFBD355B1806FB9EF8D1" box="[1062,1114,1886,1912]" italics="true" pageId="7" pageNumber="134">rpb2</emphasis>
), our isolate clustered with
<taxonomicName id="4C364D0BFFEDFFBD31F518DAFE29F835" box="[136,493,1922,1948]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD31F518DAFE29F835" box="[136,493,1922,1948]" italics="true" pageId="7" pageNumber="134">Acanthostigmina multiseptatum</emphasis>
</taxonomicName>
(ANM 665) with 91 ML/0.97 PP statistical support.
<taxonomicName id="4C364D0BFFEDFFBD353A18DAFA68F835" box="[1095,1452,1922,1948]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD353A18DAFA68F835" box="[1095,1452,1922,1948]" italics="true" pageId="7" pageNumber="134">Acanthostigmina multiseptatum</emphasis>
</taxonomicName>
was originally introduced as
<taxonomicName id="4C364D0BFFEDFFBD30A218FFFD66F869" box="[479,674,1958,1985]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD30A218FFFD66F869" box="[479,674,1958,1985]" italics="true" pageId="7" pageNumber="134">A. multiseptatum</emphasis>
</taxonomicName>
based on phylogenetic analyses along with unique morphological features, such as having longer asci and longer ascospores with more septa (
<bibRefCitation id="EFA74B79FFEDFFBD32B61892FB0AF84D" author="Promputtha, I. &amp; Miller, A. N." box="[971,1230,1994,2021]" pageId="7" pageNumber="134" pagination="574 - 587" refId="ref9903" refString="Promputtha, I. &amp; Miller, A. N. (2010) Three new species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park. Mycologia 102 (3): 574 - 587. https: // doi. org / 10.3852 / 09 - 051" type="journal article" year="2010">
Promputtha
<emphasis id="B942EA9AFFEDFFBD35281893FB41F84D" box="[1109,1157,1994,2020]" italics="true" pageId="7" pageNumber="134">et al</emphasis>
. 2010
</bibRefCitation>
). Based on morpho-molecular studies,
<bibRefCitation id="EFA74B79FFEDFFBD302B18B7FD8EF7A1" author="Boonmee, S. &amp; Rossman, A. Y. &amp; Liu, J. K. &amp; Li, W. J. &amp; Dai, D. Q. &amp; Bhat, J. D. &amp; Jones, E. G. &amp; McKenzie, E. H. &amp; Xu, J. C. &amp; Hyde, K. D." box="[342,586,2030,2057]" pageId="7" pageNumber="134" pagination="239 - 298" refId="ref6574" refString="Boonmee, S., Rossman, A. Y., Liu, J. K., Li, W. J., Dai, D. Q., Bhat, J. D., Jones, E. G., McKenzie, E. H., Xu, J. C. &amp; Hyde, K. D. (2014) Tubeufiales, ord. nov., integrating sexual and asexual generic names. Fungal Diversity 68: 239 - 298. https: // doi. org / 10.1007 / s 13225 - 014 - 0304 - 7" type="journal article" year="2014">
Boonmee
<emphasis id="B942EA9AFFEDFFBD30B818B7FE30F7A1" box="[453,500,2030,2056]" italics="true" pageId="7" pageNumber="134">et al</emphasis>
. (2014)
</bibRefCitation>
transferred this species to
<taxonomicName id="4C364D0BFFEDFFBD321518B6FBE2F7A1" box="[872,1062,2030,2056]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="genus">
<emphasis id="B942EA9AFFEDFFBD321518B6FBE2F7A1" box="[872,1062,2030,2056]" italics="true" pageId="7" pageNumber="134">Acanthostigmina</emphasis>
</taxonomicName>
. The base pair comparison between our new isolate and
<taxonomicName id="4C364D0BFFEDFFBD3013174BFDE9F785" box="[366,557,2066,2093]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFEDFFBD3013174BFDE9F785" box="[366,557,2066,2093]" italics="true" pageId="7" pageNumber="134">A. multiseptatum</emphasis>
</taxonomicName>
(ANM 665) revealed 0.99 % (526/529bp) differences in the ITS. Phylogenetic analyses coupled with morphological data, we reported our new isolate as the sexual morph of
<taxonomicName id="4C364D0BFFE2FFB235C11FC7FABFFF11" box="[1212,1403,158,185]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="8" pageNumber="135" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFE2FFB235C11FC7FABFFF11" box="[1212,1403,158,185]" italics="true" pageId="8" pageNumber="135">A. multiseptatum</emphasis>
</taxonomicName>
and the first asexual report of the genus.
<taxonomicName id="4C364D0BFFE2FFB233671F9AFCB9FF75" box="[538,893,194,220]" class="Dothideomycetes" family="Tubeufiaceae" genus="Acanthostigmina" kingdom="Fungi" order="Tubeufiales" pageId="8" pageNumber="135" phylum="Ascomycota" rank="species" species="multiseptatum">
<emphasis id="B942EA9AFFE2FFB233671F9AFCB9FF75" box="[538,893,194,220]" italics="true" pageId="8" pageNumber="135">Acanthostigmina multiseptatum</emphasis>
</taxonomicName>
was also identified as the first geographical report from
<collectingCountry id="F3217618FFE2FFB231B81FBEFEC8FEA9" box="[197,268,230,256]" name="China" pageId="8" pageNumber="135">China</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>