215 lines
16 KiB
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215 lines
16 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.65.47704" ID-GBIF-Dataset="00685a01-0785-47ec-aa0b-77c991ee4b08" ID-PMC="PMC7078340" ID-Pensoft-Pub="1314-4049-65-49" ID-Pensoft-UUID="DED2BF86E6FD56179642F7119604CEB9" ID-PubMed="32206025" ModsDocID="1314-4049-65-49" checkinTime="1583860301782" checkinUser="pensoft" docAuthor="Hou, Lingwei, Hernandez-Restrepo, Margarita, Groenewald, Johannes Zacharias, Cai, Lei & Crous, Pedro W." docDate="2020" docId="ED31092F7A615A8ABD8ECF112EDB1A15" docLanguage="en" docName="MycoKeys 65: 49-99" docOrigin="MycoKeys 65" docSource="http://dx.doi.org/10.3897/mycokeys.65.47704" docTitle="Nothophoma brennandiae Hern. - Restr., L. W. Hou, L. Cai & Crous 2020, sp. nov." docType="treatment" docVersion="4" id="DED2BF86E6FD56179642F7119604CEB9" lastPageNumber="49" masterDocId="DED2BF86E6FD56179642F7119604CEB9" masterDocTitle="Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes)" masterLastPageNumber="99" masterPageNumber="49" pageNumber="49" updateTime="1668136425574" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes)</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hou, Lingwei</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hernandez-Restrepo, Margarita</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Groenewald, Johannes Zacharias</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Cai, Lei</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Crous, Pedro W.</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2020</mods:date>
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<mods:detail type="volume">
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<mods:number>65</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>49</mods:start>
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<mods:end>99</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.65.47704</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.65.47704</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-65-49</mods:identifier>
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<mods:identifier type="Pensoft-UUID">DED2BF86E6FD56179642F7119604CEB9</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="162481716" LSID="urn:lsid:plazi:treatment:ED31092F7A615A8ABD8ECF112EDB1A15" httpUri="http://treatment.plazi.org/id/ED31092F7A615A8ABD8ECF112EDB1A15" lastPageNumber="49" pageId="0" pageNumber="49">
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<subSubSection pageId="0" pageNumber="49" type="nomenclature">
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName LSID="ED31092F-7A61-5A8A-BD8E-CF112EDB1A15" authority="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma brennandiae" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="brennandiae" status="sp. nov.">Nothophoma brennandiae Hern.-Restr., L. W. Hou, L. Cai & Crous</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="49">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Nothophoma brennandiae (CBS 145912). A, B Colony on OA (front and reverse) C, D colony on MEA (front and reverse) E, F colony on PDA (front and reverse) G pycnidia forming on OA. H, I pycnidia J section of pycnidial wall K-M conidiogenous cells N conidia. Scale bars: 50 μm (H, I); 10 μm (J); 5 μm (K-N)." figureDoi="10.3897/mycokeys.65.47704.figure6" httpUri="https://binary.pensoft.net/fig/388209" pageId="0" pageNumber="49">Figure 6</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="etymology">
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<paragraph pageId="0" pageNumber="49">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="brennandiae" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="brennandiae">
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<emphasis italics="true" pageId="0" pageNumber="49">brennandiae</emphasis>
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</taxonomicName>
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refers to Kristel Brennand who collected the soil sample from which the ex-type strain was isolated.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="typus">
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<paragraph pageId="0" pageNumber="49">Typus.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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The Netherlands. Limburg province, Ell, isolated from garden soil, Mar. 2017, K. Brennand (
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<emphasis bold="true" italics="true" pageId="0" pageNumber="49">holotype</emphasis>
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designated here CBS H-24103, living ex-type culture CBS 145912 = JW 53011).
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</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<emphasis italics="true" pageId="0" pageNumber="49">Conidiomata</emphasis>
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pycnidial, superficial to semi-immersed, solitary to confluent, globose to subglobose, irregularly-shaped with age, brown, setose, especially near the ostioles, 155-350
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<normalizedToken originalValue="×">x</normalizedToken>
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100-300
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; with 1-4 papillate ostioles; pycnidial wall pseudoparenchymatous, 3-6 layers, 13.5-21.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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thick, outer layers composed of brown, flattened polygonal cells.
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<emphasis italics="true" pageId="0" pageNumber="49">Conidiogenous cells</emphasis>
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phialidic, hyaline, smooth, ampulliform or somewhat isodiametric, 3-5
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<normalizedToken originalValue="×">x</normalizedToken>
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5-8
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="49">Conidia</emphasis>
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ellipsoidal, broadly ellipsoidal to oblong, straight, thick- and smooth-walled, hyaline becoming brown, aseptate, 3-8.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, 1-6-guttulate, minute.
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<taxonomicName genus="Conidial" lsidName="Conidial matrix" pageId="0" pageNumber="49" rank="species" species="matrix">
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<emphasis italics="true" pageId="0" pageNumber="49">Conidial matrix</emphasis>
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</taxonomicName>
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sepia to brown vinaceous.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="49">Colonies after 7 d at 25 °C, on OA reaching 50-55 mm diam, aerial mycelium scarce, spore mass with grease-like appearance, dark brick to sepia, cinnamon to the edge, abundant production of confluent pycnidia, margin entire; reverse concentric rings umber to cinnamon. On MEA reaching 47-50 mm diam, aerial mycelium scarce, spore mass with grease-like appearance, dark brick to sepia, cinnamon to the edge, abundant production of confluent pycnidia, margin entire; reverse concentric rings umber to cinnamon. On PDA reaching 50-55 mm diam, aerial mycelium moderate to scarce, cottony, buff, spore mass with grease-like appearance, dark brick, ochreous to the edge, margin entire; reverse concentric rings dark brick to cinnamon. NaOH spot test negative on OA.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="additional specimen examined">
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<paragraph pageId="0" pageNumber="49">Additional specimen examined.</paragraph>
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<paragraph pageId="0" pageNumber="49">The Netherlands. North Holland province, Amsterdam, isolated from garden soil, Mar. 2017, J. van Dijk, JW 1066.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="notes">
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<paragraph pageId="0" pageNumber="49">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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In the phylogenetic tree
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<taxonomicName lsidName="N. brennandiae" pageId="0" pageNumber="49" rank="species" species="brennandiae">
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<emphasis italics="true" pageId="0" pageNumber="49">N. brennandiae</emphasis>
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</taxonomicName>
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was close to
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<taxonomicName lsidName="N. quercina" pageId="0" pageNumber="49" rank="species" species="quercina">
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<emphasis italics="true" pageId="0" pageNumber="49">N. quercina</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="N. pruni" pageId="0" pageNumber="49" rank="species" species="pruni">
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<emphasis italics="true" pageId="0" pageNumber="49">N. pruni</emphasis>
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</taxonomicName>
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(Figure
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogenetic tree generated from the maximum-likelihood analysis based on the combined ITS, LSU, tub 2 and rpb 2 sequence alignment of Didymellaceae members. The RAxML bootstrap support values (BS), Bayesian posterior probabilities (PP), and parsimony bootstrap support values (PBS) are given at the nodes (BS / PP / PBS). BS and PBS values represent parsimony bootstrap support values> 50 %. Full supported branches are indicated in bold. The scale bar represents the expected number of changes per site. Ex-type strains are represented in bold. Strains obtained in the current study are printed in green; among them, whilst strains that represent new taxa are printed in red. Some of the basal branches were shortened to facilitate layout (the fraction in round parentheses refers to the presented length compared to the actual length of the branch). The tree was rooted to Coniothyrium palmarum CBS 400.71 and Leptosphaeria doliolum CBS 505.75." figureDoi="10.3897/mycokeys.65.47704.figure1" httpUri="https://binary.pensoft.net/fig/388204" pageId="0" pageNumber="49">1</figureCitation>
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). Morphologically,
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<taxonomicName lsidName="N. brennandiae" pageId="0" pageNumber="49" rank="species" species="brennandiae">
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<emphasis italics="true" pageId="0" pageNumber="49">N. brennandiae</emphasis>
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</taxonomicName>
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can be distinguished from
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<taxonomicName lsidName="N. quercina" pageId="0" pageNumber="49" rank="species" species="quercina">
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<emphasis italics="true" pageId="0" pageNumber="49">N. quercina</emphasis>
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</taxonomicName>
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by having setose conidiomata with up to 4 ostioles, while in
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<taxonomicName lsidName="N. quercina" pageId="0" pageNumber="49" rank="species" species="quercina">
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<emphasis italics="true" pageId="0" pageNumber="49">N. quercina</emphasis>
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</taxonomicName>
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conidiomata are glabrous with a single ostiole (
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<bibRefCitation author="Sydow, H" journalOrPublisher="Annales Mycologici" pageId="0" pageNumber="49" pagination="35 - 43" refId="B58" refString="Sydow, H, Sydow, P, 1915. Novae fungorum species. Annales Mycologici 13: 35 - 43" title="Novae fungorum species." volume="13" year="1915">Sydow and Sydow 1915</bibRefCitation>
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;
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<bibRefCitation DOI="https://doi.org/10.3114/sim.2010.65.01" author="Aveskamp, MM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="49" pagination="1 - 60" refId="B3" refString="Aveskamp, MM, de Gruyter, J, Woudenberg, JHC, Verkley, GJM, Crous, PW, 2010. Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera. Studies in Mycology 65: 1 - 60, DOI: https://doi.org/10.3114/sim.2010.65.01" title="Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera." url="https://doi.org/10.3114/sim.2010.65.01" volume="65" year="2010">Aveskamp et al. 2010</bibRefCitation>
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). Furthermore, conidia in
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<taxonomicName lsidName="N. quercina" pageId="0" pageNumber="49" rank="species" species="quercina">
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<emphasis italics="true" pageId="0" pageNumber="49">N. quercina</emphasis>
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</taxonomicName>
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are larger and have less guttules (5.5-9
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, 0-2(-3) guttules) (
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<bibRefCitation author="Sydow, H" journalOrPublisher="Annales Mycologici" pageId="0" pageNumber="49" pagination="35 - 43" refId="B58" refString="Sydow, H, Sydow, P, 1915. Novae fungorum species. Annales Mycologici 13: 35 - 43" title="Novae fungorum species." volume="13" year="1915">Sydow and Sydow 1915</bibRefCitation>
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;
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<bibRefCitation DOI="https://doi.org/10.3114/sim.2010.65.01" author="Aveskamp, MM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="49" pagination="1 - 60" refId="B3" refString="Aveskamp, MM, de Gruyter, J, Woudenberg, JHC, Verkley, GJM, Crous, PW, 2010. Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera. Studies in Mycology 65: 1 - 60, DOI: https://doi.org/10.3114/sim.2010.65.01" title="Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera." url="https://doi.org/10.3114/sim.2010.65.01" volume="65" year="2010">Aveskamp et al. 2010</bibRefCitation>
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). On the other hand,
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<taxonomicName lsidName="N. pruni" pageId="0" pageNumber="49" rank="species" species="pruni">
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<emphasis italics="true" pageId="0" pageNumber="49">N. pruni</emphasis>
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</taxonomicName>
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is characterised by hyaline conidia (
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<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/10/1/8" author="Chethana, KWT" journalOrPublisher="Mycosphere" pageId="0" pageNumber="49" pagination="490 - 530" refId="B17" refString="Chethana, KWT, Jayawardene, RS, Zhang, W, Zhou, YY, Liu, M, Hyde, KD, Li, XH, Wang, J, Zhang, KC, Yan, JY, 2019. Molecular characterization and pathogenicity of fungal taxa associated with cherry leaf spot disease. Mycosphere 10: 490 - 530, DOI: https://doi.org/10.5943/mycosphere/10/1/8" title="Molecular characterization and pathogenicity of fungal taxa associated with cherry leaf spot disease." url="https://doi.org/10.5943/mycosphere/10/1/8" volume="10" year="2019">Chethana et al. 2019</bibRefCitation>
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), while
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<taxonomicName lsidName="N. brennandiae" pageId="0" pageNumber="49" rank="species" species="brennandiae">
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<emphasis italics="true" pageId="0" pageNumber="49">N. brennandiae</emphasis>
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</taxonomicName>
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produces conidia that turn brown with age.
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</paragraph>
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<caption doi="10.3897/mycokeys.65.47704.figure6" httpUri="https://binary.pensoft.net/fig/388209" pageId="0" pageNumber="49" start="Figure 6" startId="F6">
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<paragraph pageId="0" pageNumber="49">
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<emphasis bold="true" pageId="0" pageNumber="49">Figure 6.</emphasis>
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<taxonomicName authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Nothophoma" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nothophoma brennandiae" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="brennandiae">
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<emphasis italics="true" pageId="0" pageNumber="49">Nothophoma brennandiae</emphasis>
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</taxonomicName>
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(CBS 145912).
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<emphasis bold="true" pageId="0" pageNumber="49">A, B</emphasis>
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Colony on OA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">C, D</emphasis>
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colony on MEA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">E, F</emphasis>
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colony on PDA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">G</emphasis>
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pycnidia forming on OA.
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<emphasis bold="true" pageId="0" pageNumber="49">H, I</emphasis>
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pycnidia
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<emphasis bold="true" pageId="0" pageNumber="49">J</emphasis>
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section of pycnidial wall
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<emphasis bold="true" pageId="0" pageNumber="49">K-M</emphasis>
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conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="49">N</emphasis>
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conidia. Scale bars: 50
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">H, I</emphasis>
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); 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">J</emphasis>
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); 5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">K-N</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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</treatment>
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</document> |