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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.71.56168" ID-GBIF-Dataset="fc10c6bc-400a-43ac-b1b5-061e9dcc0705" ID-PMC="PMC7426279" ID-Pensoft-Pub="1314-4049-71-87" ID-Pensoft-UUID="40ACEC39417B5B5C992701CA9F4E626D" ID-PubMed="32855604" ModsDocID="1314-4049-71-87" checkinTime="1596691551541" checkinUser="pensoft" docAuthor="Jia, Ran-Ran, Lv, Shi-Long, Chai, Chun-Yue &amp; Hui, Feng-Li" docDate="2020" docId="EEE03D3AE86D557283EAEE537A01F970" docLanguage="en" docName="MycoKeys 71: 87-99" docOrigin="MycoKeys 71" docSource="http://dx.doi.org/10.3897/mycokeys.71.56168" docTitle="Scheffersomyces jinghongensis C. Y. Chai &amp; F. L. Hui 2020, sp. nov." docType="treatment" docVersion="4" id="40ACEC39417B5B5C992701CA9F4E626D" lastPageNumber="91" masterDocId="40ACEC39417B5B5C992701CA9F4E626D" masterDocTitle="Three new Scheffersomyces species associated with insects and rotting wood in China" masterLastPageNumber="99" masterPageNumber="87" pageNumber="91" updateTime="1668136524283" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Three new Scheffersomyces species associated with insects and rotting wood in China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jia, Ran-Ran</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Lv, Shi-Long</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chai, Chun-Yue</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hui, Feng-Li</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
<mods:nameIdentifier type="email">fenglihui@yeah.net</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2020</mods:date>
<mods:detail type="volume">
<mods:number>71</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>87</mods:start>
<mods:end>99</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.71.56168</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.71.56168</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-71-87</mods:identifier>
<mods:identifier type="Pensoft-UUID">40ACEC39417B5B5C992701CA9F4E626D</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="166095967" LSID="urn:lsid:plazi:treatment:EEE03D3AE86D557283EAEE537A01F970" httpUri="http://treatment.plazi.org/id/EEE03D3AE86D557283EAEE537A01F970" lastPageNumber="91" pageId="4" pageNumber="91">
<subSubSection pageId="4" pageNumber="91" type="nomenclature">
<paragraph pageId="4" pageNumber="91">
<taxonomicName LSID="EEE03D3A-E86D-5572-83EA-EE537A01F970" authority="C. Y. Chai &amp; F. L. Hui" authorityName="C. Y. Chai &amp; F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Saccharomycetaceae" genus="Scheffersomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Scheffersomyces jinghongensis" order="Saccharomycetales" pageId="4" pageNumber="91" phylum="Ascomycota" rank="species" species="jinghongensis" status="sp. nov.">Scheffersomyces jinghongensis C.Y. Chai &amp; F.L. Hui</taxonomicName>
<taxonomicNameLabel pageId="4" pageNumber="91">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of S. jinghongensis. A budding cells B pseudohyphae. Scale bars: 10 μm." httpUri="https://binary.pensoft.net/fig/439461" pageId="4" pageNumber="91">Figure 2</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="etymology">
<paragraph pageId="4" pageNumber="91">Etymology.</paragraph>
<paragraph pageId="4" pageNumber="91">
The species name
<taxonomicName authorityName="C. Y. Chai &amp; F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Debaryomycetaceae" genus="Scheffersomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="jinghongensis" order="Saccharomycetales" pageId="4" pageNumber="91" phylum="Ascomycota" rank="species" species="jinghongensis">
<emphasis italics="true" pageId="4" pageNumber="91">jinghongensis</emphasis>
</taxonomicName>
(N.L. fem. adj.) refers to the geographical origin of the type strain of this species.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="holotype">
<paragraph pageId="4" pageNumber="91">Holotype.</paragraph>
<paragraph pageId="4" pageNumber="91">NYNU 17926T.</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="isolation data">
<paragraph pageId="4" pageNumber="91">Isolation data.</paragraph>
<paragraph pageId="4" pageNumber="91">China, Yunnan Province, Jinghong, in rotting wood, under a tropical rainforest, July 2017, K.F. Liu &amp; Z.W. Xi (ex-holotype: CICC 33270; CBS 15230).</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="description">
<paragraph pageId="4" pageNumber="91">Description.</paragraph>
<paragraph pageId="4" pageNumber="91">
The cells are ovoid to elongate (3-4
<normalizedToken originalValue="×">x</normalizedToken>
3-7.5
<normalizedToken originalValue="μm">μm</normalizedToken>
) and occur singly or in pairs after being placed in YM broth for 3 days at 25 °C (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of S. jinghongensis. A budding cells B pseudohyphae. Scale bars: 10 μm." httpUri="https://binary.pensoft.net/fig/439461" pageId="4" pageNumber="91">2A</figureCitation>
). Budding is multilateral. After 3 days of growth on YM agar at 25 °C, the colonies are white to cream-colored, buttery, and smooth, with entire margins. After 7 days at 25 °C on a Dalmau plate culture with CM agar, pseudohyphae were observed but true hyphae were not (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of S. jinghongensis. A budding cells B pseudohyphae. Scale bars: 10 μm." httpUri="https://binary.pensoft.net/fig/439461" pageId="4" pageNumber="91">2B</figureCitation>
). Asci or signs of conjugation were not observed on sporulation media. Glucose, galactose, trehalose, cellobiose, and d-xylose (weak) are fermented, but maltose, sucrose, melibiose, lactose, melezitose, raffinose, and inulin are not. Glucose, galactose, l-sorbose, d-glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, cellobiose, arbutin, melezitose, inulin, glycerol, erythritol, ribitol, xylitol, d-glucitol, d-mannitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, d-glucuronate, succinate, and ethanol are assimilated. No growth was observed in l-arabinose, d-arabinose, l-rhamnose, methyl
<normalizedToken originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
, salicin, melibiose, lactose, raffinose, l-arabinitol, galactitol,
<emphasis italics="true" pageId="4" pageNumber="91">myo</emphasis>
-inositol, 5-keto-d-gluconate, d-gluconate, dl-lactate, citrate, or methanol. For the assimilation of nitrogen compounds, growth on l-lysine, glucosamine, or d-tryptophan is positive, while growth on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine, or imidazole is negative. Growth is observed at 37 °C but not at 40 °C. Growth in the presence of 0.1% cycloheximide is positive, but growth in the presence of 10% NaCl with 5% glucose and 1% acetic acid is negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are also negative.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="additional isolate examined">
<paragraph pageId="4" pageNumber="91">Additional isolate examined.</paragraph>
<paragraph pageId="4" pageNumber="91">China, Yunnan Province, Jinghong, in rotting wood, under a tropical rainforest, July 2017, K.F. Liu &amp; Z.W. Xi, NYNU 17977.</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="genbank accession numbers">
<paragraph pageId="4" pageNumber="91">GenBank accession numbers.</paragraph>
<paragraph pageId="4" pageNumber="91">holotype NYNU 17926T (ITS:MG255722; nrLSU D1/D2: MG255714); additional isolate NYNU 17977 (ITS: MT133547; nrLSU D1/D2: MT133543).</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="91" type="notes">
<paragraph pageId="4" pageNumber="91">Notes.</paragraph>
<paragraph pageId="4" pageNumber="91">
Two strains representing
<taxonomicName lsidName="S. jinghongensis" pageId="4" pageNumber="91" rank="species" species="jinghongensis">
<emphasis italics="true" pageId="4" pageNumber="91">S. jinghongensis</emphasis>
</taxonomicName>
were grouped in an independent lineage and are related to
<taxonomicName lsidName="S. titanus" pageId="4" pageNumber="91" rank="species" species="titanus">
<emphasis italics="true" pageId="4" pageNumber="91">S. titanus</emphasis>
</taxonomicName>
and other
<taxonomicName authorityName="C.P.Kurtzman &amp; M.Suzuki" authorityYear="2010" class="Saccharomycetes" family="Debaryomycetaceae" genus="Scheffersomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Scheffersomyces" order="Saccharomycetales" pageId="4" pageNumber="91" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="4" pageNumber="91">Scheffersomyces</emphasis>
</taxonomicName>
species. The nucleotide differences between the new species and the close relative
<taxonomicName lsidName="S. titanus" pageId="4" pageNumber="91" rank="species" species="titanus">
<emphasis italics="true" pageId="4" pageNumber="91">S. titanus</emphasis>
</taxonomicName>
(
<bibRefCitation DOI="https://doi.org/10.1038/srep32181" author="Liu, XJ" journalOrPublisher="International Journal of Systematic and Evolutionary Microbiology" pageId="10" pageNumber="97" refId="B16" refString="Liu, XJ, Cao, WN, Ren, YC, Xu, LL, Yi, ZH, Liu, Z, Hui, FL, 2016b. Taxonomy and physiological characterisation of Scheffersomycestitanus sp. nov., a new D-xylose-fermenting yeast species from China. Scientific Reports 6: 32181. https://doi.org/10.1038/srep32181" title="Taxonomy and physiological characterisation of Scheffersomycestitanus sp. nov., a new D-xylose-fermenting yeast species from China. Scientific Reports 6: 32181." url="https://doi.org/10.1038/srep32181" year="2016 b">Liu et al. 2016b</bibRefCitation>
) are 1.6% substitutions in the D1/D2 domain and 4.9% substitutions in the ITS region, respectively. Physiologically,
<taxonomicName lsidName="S. jinghongensis" pageId="4" pageNumber="91" rank="species" species="jinghongensis">
<emphasis italics="true" pageId="4" pageNumber="91">S. jinghongensis</emphasis>
</taxonomicName>
can be differentiated from
<taxonomicName lsidName="S. titanus" pageId="4" pageNumber="91" rank="species" species="titanus">
<emphasis italics="true" pageId="4" pageNumber="91">S. titanus</emphasis>
</taxonomicName>
based on growth in l-arabinose, l-rhamnose, methyl
<normalizedToken originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
, salicin, lactose,
<emphasis italics="true" pageId="4" pageNumber="91">myo</emphasis>
-inositol, and 5-keto-d-gluconate, all of which were positive for
<taxonomicName lsidName="S. titanus" pageId="4" pageNumber="91" rank="species" species="titanus">
<emphasis italics="true" pageId="4" pageNumber="91">S. titanus</emphasis>
</taxonomicName>
and negative for the new species. Additionally,
<taxonomicName lsidName="S. jinghongensis" pageId="4" pageNumber="91" rank="species" species="jinghongensis">
<emphasis italics="true" pageId="4" pageNumber="91">S. jinghongensis</emphasis>
</taxonomicName>
ferments cellobiose and grows at 37 °C, but not for
<taxonomicName lsidName="S. titanus" pageId="4" pageNumber="91" rank="species" species="titanus">
<emphasis italics="true" pageId="4" pageNumber="91">S. titanus</emphasis>
</taxonomicName>
.
</paragraph>
<caption httpUri="https://binary.pensoft.net/fig/439461" pageId="4" pageNumber="91" start="Figure 2" startId="F2">
<paragraph pageId="4" pageNumber="91">
<emphasis bold="true" pageId="4" pageNumber="91">Figure 2.</emphasis>
Morphology of
<taxonomicName lsidName="S. jinghongensis" pageId="4" pageNumber="91" rank="species" species="jinghongensis">
<emphasis italics="true" pageId="4" pageNumber="91">S. jinghongensis</emphasis>
</taxonomicName>
.
<emphasis bold="true" pageId="4" pageNumber="91">A</emphasis>
budding cells
<emphasis bold="true" pageId="4" pageNumber="91">B</emphasis>
pseudohyphae. Scale bars: 10
<normalizedToken originalValue="μm">μm</normalizedToken>
.
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>