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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.33.23703" ID-GBIF-Dataset="d1f6ee7f-7311-4b74-8c3a-c99a1855ac0a" ID-PMC="PMC5911683" ID-Pensoft-Pub="1314-4049--103" ID-PubMed="29686503" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049--103" ModsDocOrigin="MycoKeys " ModsDocTitle="Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations" checkinTime="1553125629810" checkinUser="pensoft" docAuthor="Chakraborty, Dyutiparna, Vizzini, Alfredo &amp; Das, Kanad" docDate="2018" docId="2C7C7010F4EF5F893205097FBC105C20" docLanguage="en" docName="MycoKeys 33: 103-124" docOrigin="MycoKeys 33" docSource="http://dx.doi.org/10.3897/mycokeys.33.23703" docTitle="Tylopilus pseudoballoui K. Das, D. Chakr &amp; Vizzini, sp. nov." docType="treatment" docVersion="4" lastPageNumber="111" masterDocId="FF96FFD19A54FF8F0134FFD9FFE6FF83" masterDocTitle="Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations" masterLastPageNumber="124" masterPageNumber="103" pageNumber="109" updateTime="1668135975692" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chakraborty, Dyutiparna</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Vizzini, Alfredo</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Das, Kanad</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>33</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>103</mods:start>
<mods:end>124</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.33.23703</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.33.23703</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049--103</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="154474058" LSID="urn:lsid:plazi:treatment:2C7C7010F4EF5F893205097FBC105C20" httpUri="http://treatment.plazi.org/id/2C7C7010F4EF5F893205097FBC105C20" lastPageId="9" lastPageNumber="111" pageId="6" pageNumber="109">
<subSubSection pageId="6" pageNumber="109" type="nomenclature">
<paragraph pageId="6" pageNumber="109">
<taxonomicName LSID="MB823977" authority="K. Das, D. Chakr &amp; Vizzini" class="Agaricomycetes" family="Boletaceae" genus="Tylopilus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tylopilus pseudoballoui" order="Boletales" pageId="6" pageNumber="109" phylum="Basidiomycota" rank="species" species="pseudoballoui">Tylopilus pseudoballoui K. Das, D. Chakr &amp; Vizzini</taxonomicName>
<taxonomicNameLabel pageId="6" pageNumber="109">sp. nov.</taxonomicNameLabel>
Figs 5, 6
</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="110" type="materials_examined">
<paragraph pageId="7" pageNumber="110">
<pageBreakToken pageId="7" pageNumber="110" start="start">Type</pageBreakToken>
.
</paragraph>
<paragraph pageId="7" pageNumber="110">
INDIA. Sikkim, South District, Maenam WLS (Maenum 3), 2136 m alt.,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="27.25964">N27°15'34.7&quot;</geoCoordinate>
<geoCoordinate direction="east" orientation="longitude" precision="1" value="88.35714">E88°21'25.7&quot;</geoCoordinate>
, 23 Aug 2017,
<taxonomicName class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="7" pageNumber="110" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
spp., D. Chakraborty &amp; K. Das, DC 17-30 (CAL 1651)
</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="110" type="diagnosis">
<paragraph pageId="7" pageNumber="110">Diagnosis.</paragraph>
<paragraph pageId="7" pageNumber="110">Distinct from all allied taxa by sequence data (nrITS and nrLSU) and morphologically by its sticky orange-yellow pileus surface, pale yellow pore surface which turns to pale orange or greyish-orange when bruised and absence of reticulation on stipe surface.</paragraph>
</subSubSection>
<subSubSection pageId="7" pageNumber="110" type="etymology">
<paragraph pageId="7" pageNumber="110">Etymology.</paragraph>
<paragraph pageId="7" pageNumber="110">
referring to its being a look-alike of
<taxonomicName lsidName="T. balloui" pageId="7" pageNumber="110" rank="species" species="balloui">T. balloui</taxonomicName>
, a North American species.
</paragraph>
</subSubSection>
<subSubSection lastPageId="8" lastPageNumber="111" pageId="7" pageNumber="110" type="description">
<paragraph pageId="7" pageNumber="110">Description.</paragraph>
<paragraph pageId="7" pageNumber="110">
Pileus 60-150 mm diam., initially convex then plano-convex, surface sticky, orange-yellow to brownish-yellow (5
<normalizedToken originalValue="BC">B-C</normalizedToken>
8), paler at margin; margin entire, plain, without any sterile flap of tissue. Pore surface pale yellow (3A3), turning pale orange to greyish-orange (6
<normalizedToken originalValue="AB">A-B</normalizedToken>
3) on bruising; pores angular, 5-8/mm. Tubes subdecurrent, 6-10 mm long, yellowish-white, brownish on bruising. Stipe 55-110
<normalizedToken originalValue="×">x</normalizedToken>
20-40 mm, mostly subclavate, solid, concolorous with pileus; surface pruinose, never reticulate; basal mycelium white. Context 20 mm thick in pileus, chalky white (1A1), unchanging on exposure but turning turquoise grey (24D2-D1) with FeSO4 (chalk), pale yellow (4A3) with 5% KOH, yellowish-grey (4B3) in Guaiacol. Pileus surface brownish-red (8C8-7) on bruising, turning dark green to greenish-grey (25
<normalizedToken originalValue="EF3">E-F3-</normalizedToken>
2) in FeSO4, vivid yellow (3A8) in KOH, unchanging in NH4OH. Stipe 55-110
<normalizedToken originalValue="×">x</normalizedToken>
20-40 mm, mostly subclavate, solid, concolorous with pileus; surface pruinose, never reticulate; basal mycelium white. Odour pleasant. Taste slightly pungent. Spore print not obtained.
</paragraph>
<paragraph lastPageId="8" lastPageNumber="111" pageId="7" pageNumber="110">
Basidiospores 6.4
<normalizedToken originalValue="7.4">-7.4-</normalizedToken>
9.9
<normalizedToken originalValue="×">x</normalizedToken>
3.8
<normalizedToken originalValue="4.5">-4.5-</normalizedToken>
5.7
<normalizedToken originalValue="µm">µm</normalizedToken>
(n = 30, Q = 1.5
<normalizedToken originalValue="1.73">-1.73-</normalizedToken>
2.04), ellipsoidal, thin-walled, smooth under light microscope. Basidia 22- 30
<normalizedToken originalValue="×">x</normalizedToken>
8-10
<normalizedToken originalValue="µm">µm</normalizedToken>
, four-spored, clavate. Pleurocystidia 40-54
<normalizedToken originalValue="×">x</normalizedToken>
7-10
<normalizedToken originalValue="µm">µm</normalizedToken>
, emergent up to 30
<normalizedToken originalValue="µm">µm</normalizedToken>
, fusoid to ventricose, appendiculate, yellow pigmented or hyaline, mostly with dense globular to oily content. Tube edge fertile; cheilocystidia 33-55
<normalizedToken originalValue="×">x</normalizedToken>
7-10
<normalizedToken originalValue="µm">µm</normalizedToken>
, common, clavate to
<pageBreakToken pageId="8" pageNumber="111" start="start">cylindrical</pageBreakToken>
, subfusoid to ventricose. Hymenophoral trama divergent, hyphae septate, gelatinous, up to 5
<normalizedToken originalValue="µm">µm</normalizedToken>
wide. Pileipellis an ixocutis up to 150-280
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, composed of subparallel to suberect, somewhat interwoven hyphae; terminal elements 20-70
<normalizedToken originalValue="×">x</normalizedToken>
6-11
<normalizedToken originalValue="µm">µm</normalizedToken>
, cylindrical to subcylindrical, sometimes subfusoid, content orange-brown pigmented. Stipitipellis up to 150
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, fertile, composed of basidia and cystidia in several clusters; caulobasidia similar to that of hymenial basidia; caulocystidia 40-76
<normalizedToken originalValue="×">x</normalizedToken>
10-12
<normalizedToken originalValue="µm">µm</normalizedToken>
, broadly clavate to subclavate or appendiculate.
</paragraph>
<caption pageId="8" pageNumber="111">
<paragraph pageId="8" pageNumber="111">
Figure 5.
<taxonomicName class="Agaricomycetes" family="Boletaceae" genus="Tylopilus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tylopilus pseudoballoui" order="Boletales" pageId="8" pageNumber="111" phylum="Basidiomycota" rank="species" species="pseudoballoui">Tylopilus pseudoballoui</taxonomicName>
(DC 17-30, holotype). a, c Fresh basidiomata in the field b Pore surface after bruising d Hymenophoral trama e Pleurocystidia f Pileipellis g Stipitipellis h Basidiospores. Scale bars: 100
<normalizedToken originalValue="μm">μm</normalizedToken>
(d, f); 50
<normalizedToken originalValue="μm">μm</normalizedToken>
(g); 10 mm (e, h).
</paragraph>
</caption>
<caption pageId="8" pageNumber="111">
<paragraph pageId="8" pageNumber="111">
Figure 6.
<taxonomicName class="Agaricomycetes" family="Boletaceae" genus="Tylopilus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tylopilus pseudoballoui" order="Boletales" pageId="8" pageNumber="111" phylum="Basidiomycota" rank="species" species="pseudoballoui">Tylopilus pseudoballoui</taxonomicName>
(DC 17-30, holotype). a Basidiospores b Basidia c Caulocystidia d Pleurocystidia e Pileipellis. Scale bars: 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="ae">a-e</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection lastPageId="9" lastPageNumber="112" pageId="8" pageNumber="111" type="materials_examined">
<paragraph pageId="8" pageNumber="111">Specimens examined.</paragraph>
<paragraph lastPageId="9" lastPageNumber="112" pageId="8" pageNumber="111">
India. Sikkim: South District, Maenam WLS (Maenam 3), 2136 m alt.,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="27.25964">N27°15'34.7&quot;</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="88.35714">E88°21'25.7&quot;</geoCoordinate>
, 23 Aug 2017,
<taxonomicName class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="8" pageNumber="111" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp.,
<pageBreakToken pageId="9" pageNumber="112" start="start">D</pageBreakToken>
. Chakraborty &amp; K. Das, DC 17-35 (CAL); Uttarakhand: Champawat district, Abbot mount, 1885 m alt.,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="29.424433">N29°25.466'</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="80.10142">E80°06.085'</geoCoordinate>
, 18 July 2017, A. Ghosh, KD B-02 (CAL). Uttarakhand: Pauri district, 1971 m alt.,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="30.0479">N30°02.874'</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="79.13702">E79°08.221'</geoCoordinate>
, 4 Aug 2017, K. Das, KD 17-24 (CAL).
</paragraph>
</subSubSection>
</treatment>
</document>