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107 lines
11 KiB
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<document id="EC4F7C0A53322923BED36CAB16A95AD1" ID-DOI="10.1016/j.phytochem.2018.10.027" ID-ISSN="1873-3700" ID-Zenodo-Dep="10481193" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="karina" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="karina" IM.treatments_approvedBy="karina" checkinTime="1704925253971" checkinUser="felipe" docAuthor="Andersen, Trine Bundgaard, Rasmussen, Silas Anselm, Christensen, Søren Brøgger & Simonsen, Henrik Toft" docDate="2019" docId="039087C3FF9B4065FC96750BE47EDA7B" docLanguage="en" docName="Phytochemistry.157.168-174.pdf" docOrigin="Phytochemistry 157" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.10.027" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Nicotiana benthamiana" docType="treatment" docVersion="5" lastPageNumber="169" masterDocId="FFA9FFBBFF9A4067FFA47619E55DDE50" masterDocTitle="Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy" masterLastPageNumber="174" masterPageNumber="168" pageNumber="169" updateTime="1706402960486" updateUser="ExternalLinkService">
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<mods:mods id="2EB613AF493FCEF35DA5553CDD45EB4F" xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo id="7505DC55FC6BAF201EF1DAC988C3B193">
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<mods:title id="BC917EEFA85CBC9142D7137317D5ED41">Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy</mods:title>
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<mods:name id="42B77D13C471DA5B2416AAD9CC013C75" type="personal">
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<mods:roleTerm id="B454BA112D8F1855B15DCA163C29664B">Author</mods:roleTerm>
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<mods:namePart id="C89EE39C1616F25EF6590BDC80CD6247">Andersen, Trine Bundgaard</mods:namePart>
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<mods:affiliation id="F2EDD97518FD4C0BE33CAD05104085F1">Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark</mods:affiliation>
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<mods:namePart id="9BA6FC9F9BD833A40E83E07CB3B1B765">Rasmussen, Silas Anselm</mods:namePart>
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<mods:namePart id="C3258F55708B70C42216395EE3FB3B1F">Christensen, Søren Brøgger</mods:namePart>
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<mods:roleTerm id="3CF33C54CC5259C7DB2AB35ACF928F34">Author</mods:roleTerm>
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<mods:namePart id="021CF95C51188CA4B39EEC5B8FBD2A2D">Simonsen, Henrik Toft</mods:namePart>
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<mods:typeOfResource id="AF78D06E9A814677AE746F464BEBA6E3">text</mods:typeOfResource>
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<mods:title id="EB9E5C13D0FADA92AEC7317A9F23C1EB">Phytochemistry</mods:title>
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<mods:part id="D2CA83E8F52A52ED3D24CDE0ED588C1F">
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<mods:date id="3EC3B1690FC4053344684505B74B9115">2019</mods:date>
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<mods:number id="32048619A51CB95A9ECC8FD481860A9A">2019-01-31</mods:number>
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<mods:number id="3DBE2E7B719BEC73FD9E0AAB7A2C0835">157</mods:number>
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<mods:start id="88D95FF070D475BF8504325B90002EEE">168</mods:start>
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<mods:end id="D3CB87C65BF6DC590B7F112817B2F4E3">174</mods:end>
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<mods:url id="C173D8E183AB7B9AE665D0B2BC0AB224">http://dx.doi.org/10.1016/j.phytochem.2018.10.027</mods:url>
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<mods:classification id="C3C4519C97DD4DE9BC65C59EC50C04A2">journal article</mods:classification>
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<mods:identifier id="7E4D739E02C0C019CC646A1326E1E51B" type="DOI">10.1016/j.phytochem.2018.10.027</mods:identifier>
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<mods:identifier id="605C50418C4328F160F29CEB921AB6C7" type="ISSN">1873-3700</mods:identifier>
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<mods:identifier id="76361118540796E6987FBFA2851AA507" type="Zenodo-Dep">10481193</mods:identifier>
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<treatment id="039087C3FF9B4065FC96750BE47EDA7B" ID-DOI="http://doi.org/10.5281/zenodo.10576565" ID-Zenodo-Dep="10576565" LSID="urn:lsid:plazi:treatment:039087C3FF9B4065FC96750BE47EDA7B" httpUri="http://treatment.plazi.org/id/039087C3FF9B4065FC96750BE47EDA7B" lastPageId="2" lastPageNumber="169" pageId="1" pageNumber="169">
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<subSubSection id="C323655EFF9B4066FC96750BE02BDD75" box="[818,1398,786,805]" pageId="1" pageNumber="169" type="nomenclature">
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<paragraph id="8B8636D5FF9B4066FC96750BE02BDD75" blockId="1.[818,1398,786,805]" box="[818,1398,786,805]" pageId="1" pageNumber="169">
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<heading id="D0CE81B9FF9B4066FC96750BE02BDD75" bold="true" box="[818,1398,786,805]" fontSize="36" level="1" pageId="1" pageNumber="169" reason="1">
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<emphasis id="B94DEAC7FF9B4066FC96750BE02BDD75" bold="true" box="[818,1398,786,805]" italics="true" pageId="1" pageNumber="169">
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2.3. Characterization of TlTPS509 expressed in
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<taxonomicName id="4C394D56FF9B4066FB43750BE02BDD75" ID-CoL="47D27" authority="Domin" box="[1255,1398,786,805]" class="Magnoliopsida" family="Solanaceae" genus="Nicotiana" kingdom="Plantae" order="Solanales" pageId="1" pageNumber="169" phylum="Tracheophyta" rank="species" species="benthamiana">N. benthamiana</taxonomicName>
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C323655EFF9B4065FCF77553E47EDA7B" lastPageId="2" lastPageNumber="170" pageId="1" pageNumber="169" type="description">
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<paragraph id="8B8636D5FF9B4065FCF77553E47EDA7B" blockId="1.[818,1488,842,1196]" lastBlockId="2.[100,770,965,1067]" lastPageId="2" lastPageNumber="170" pageId="1" pageNumber="169">
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HS-SPME-GC-MS analysis of
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<taxonomicName id="4C394D56FF9B4066FBD97553E049DD0D" box="[1149,1300,842,861]" class="Magnoliopsida" family="Solanaceae" genus="Nicotiana" kingdom="Plantae" order="Solanales" pageId="1" pageNumber="169" phylum="Tracheophyta" rank="species" species="benthamiana">
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<emphasis id="B94DEAC7FF9B4066FBD97553E049DD0D" bold="true" box="[1149,1300,842,861]" italics="true" pageId="1" pageNumber="169">N. benthamiana</emphasis>
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</taxonomicName>
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leaves expressing
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<emphasis id="B94DEAC7FF9B4066FC96757FE619DD29" bold="true" box="[818,836,870,889]" italics="true" pageId="1" pageNumber="169">Tl</emphasis>
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TPS509 resulted in the detection of two major sesquiterpene alcohols, guaiol (
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<emphasis id="B94DEAC7FF9B4066FCDE759BE6DBDDC5" bold="true" box="[890,902,898,917]" pageId="1" pageNumber="169">3</emphasis>
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) (RI: 1585, 34% of the area) and bulnesol (
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<emphasis id="B94DEAC7FF9B4066FA81759BE06CDDC5" bold="true" box="[1317,1329,898,917]" pageId="1" pageNumber="169">4</emphasis>
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) (RI: 1654, 20% of the area) (
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<figureCitation id="13022A50FF9B4066FC147584E6BBDDE0" box="[944,998,925,944]" captionStart="Fig" captionStartId="2.[100,130,825,842]" captionTargetBox="[264,1322,155,800]" captionTargetId="figure-761@2.[263,1325,152,802]" captionTargetPageId="2" captionText="Fig. 3. HS-SPME GC-MS analysis of TlTPS509 expressed together AtHMGR, the control shows the expression of AtHMGR alone. Guaiol (3) is seen as the major product (Rt 9.89 min), with bulnesol (4), Rt: 10.2, a-guaiene (Rt: 8.6), and a-bulnesene (Rt: 9.18) as minor products. * show unidentified sesquiterpene like products, which is either ketone formed during the biosynthesis or breakdown products of the alcohols formed during the GC-MS analysis. Based on this TlTPS509 is a guaiol synthase. MS spectra are presented in supplementary data." figureDoi="http://doi.org/10.5281/zenodo.10481199" httpUri="https://zenodo.org/record/10481199/files/figure.png" pageId="1" pageNumber="169">Fig. 3</figureCitation>
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). Compound
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<emphasis id="B94DEAC7FF9B4066FBCC7584E129DDE0" bold="true" box="[1128,1140,925,944]" pageId="1" pageNumber="169">3</emphasis>
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and
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<emphasis id="B94DEAC7FF9B4066FB027584E1EFDDE0" bold="true" box="[1190,1202,925,944]" pageId="1" pageNumber="169">4</emphasis>
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exhibited similar EI MS spectrum to those of bulnesol and guaiol in NIST and Wiley GC-MS spectrum libraries. The product profile showed several additional compounds, and we could identify two minor products; guaiene (RI 1418, Rt: 8.6)) and bulnesene (RI 1487, Rt: 9.18) (
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<figureCitation id="13022A50FF9B4066FB247214E1E8DA70" box="[1152,1205,1037,1056]" captionStart="Fig" captionStartId="2.[100,130,825,842]" captionTargetBox="[264,1322,155,800]" captionTargetId="figure-761@2.[263,1325,152,802]" captionTargetPageId="2" captionText="Fig. 3. HS-SPME GC-MS analysis of TlTPS509 expressed together AtHMGR, the control shows the expression of AtHMGR alone. Guaiol (3) is seen as the major product (Rt 9.89 min), with bulnesol (4), Rt: 10.2, a-guaiene (Rt: 8.6), and a-bulnesene (Rt: 9.18) as minor products. * show unidentified sesquiterpene like products, which is either ketone formed during the biosynthesis or breakdown products of the alcohols formed during the GC-MS analysis. Based on this TlTPS509 is a guaiol synthase. MS spectra are presented in supplementary data." figureDoi="http://doi.org/10.5281/zenodo.10481199" httpUri="https://zenodo.org/record/10481199/files/figure.png" pageId="1" pageNumber="169">Fig. 3</figureCitation>
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). Several peaks with a
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<emphasis id="B94DEAC7FF9B4066FA367214E0EADA70" bold="true" box="[1426,1463,1037,1056]" italics="true" pageId="1" pageNumber="169">m/z</emphasis>
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of 220 were also observed but not identified. A hexane extract of
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<taxonomicName id="4C394D56FF9B4066FA297230E6DBDA08" class="Magnoliopsida" family="Solanaceae" genus="Nicotiana" kingdom="Plantae" order="Solanales" pageId="1" pageNumber="169" phylum="Tracheophyta" rank="species" species="benthamiana">
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<emphasis id="B94DEAC7FF9B4066FA297230E6DBDA08" bold="true" italics="true" pageId="1" pageNumber="169">N. benthamiana</emphasis>
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</taxonomicName>
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leaves, expressing
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<emphasis id="B94DEAC7FF9B4066FB99725CE112DA08" bold="true" box="[1085,1103,1093,1112]" italics="true" pageId="1" pageNumber="169">Tl</emphasis>
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TPS509 was analyzed by GC-MS using a Programmed Temperature Vaporising injector (PTV) port. By using a low initial temperature of 60 ̊C followed by a gradual increase to 250 ̊C it was expected that thermal rearrangements were avoided. Again,
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<emphasis id="B94DEAC7FF984065FF0275DCE5EFDD88" bold="true" box="[166,178,965,984]" pageId="2" pageNumber="170">3</emphasis>
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and
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<emphasis id="B94DEAC7FF984065FF4575DCE5B0DD88" bold="true" box="[225,237,965,984]" pageId="2" pageNumber="170">4</emphasis>
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were detected as major compounds, but not guaiene and bulnesene, which were probably below the detection limit. Furthermore, neither of the unknown compounds with
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<emphasis id="B94DEAC7FF984065FD8D75E5E713DA5F" bold="true" box="[553,590,1020,1039]" italics="true" pageId="2" pageNumber="170">m/z</emphasis>
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220 were detected in the PTV analysis.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |