274 lines
25 KiB
XML
274 lines
25 KiB
XML
<document id="FBEDADE19ED65BFB5A791065523F1641" ID-CLB-Dataset="26393" ID-DOI="http://dx.doi.org/10.3897/mycokeys.55.35682" ID-GBIF-Dataset="2d8b9780-ff44-445d-a275-c813d05775a7" ID-PMC="PMC6609996" ID-Pensoft-Pub="1314-4049-55-59" ID-PubMed="31303813" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049-55-59" ModsDocOrigin="MycoKeys 55" ModsDocTitle="Extensive sampling and high-throughput sequencing reveal Posidoniomycesatricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidoniaoceanica" checkinTime="1561619010070" checkinUser="pensoft" docAuthor="Vohnik, Martin, Borovec, Ondrej, Kolarikova, Zuzana, Sudova, Radka & Reblova, Martina" docDate="2019" docId="8DC644221D9F7331E98EB4C9958E06E1" docLanguage="en" docName="MycoKeys 55: 59-86" docOrigin="MycoKeys 55" docSource="http://dx.doi.org/10.3897/mycokeys.55.35682" docTitle="Posidoniomyces atricolor Vohnik & Reblova, sp. nov." docType="treatment" docVersion="6" lastPageNumber="59" masterDocId="E46CD93BFF9DFFC0FF9FFFF1FFDE6957" masterDocTitle="Extensive sampling and high-throughput sequencing reveal Posidoniomycesatricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidoniaoceanica" masterLastPageNumber="86" masterPageNumber="59" pageNumber="59" updateTime="1732848644453" updateUser="ExternalLinkService">
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<mods:title id="838D6B869EC6F64180BFC7811E7AFA8B">Extensive sampling and high-throughput sequencing reveal Posidoniomycesatricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidoniaoceanica</mods:title>
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<mods:namePart id="BFF6015BD9AAA17BCAA5D9CA53755183">Vohnik, Martin</mods:namePart>
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<mods:namePart id="4813127DAD2A93A4659B109AF43B705B">Borovec, Ondrej</mods:namePart>
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<mods:namePart id="1893DB84DE6569ABD3C26DC6B71E1BDB">Kolarikova, Zuzana</mods:namePart>
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<mods:namePart id="B87C6425EDC591AB9764016A829C2736">Sudova, Radka</mods:namePart>
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<mods:namePart id="5320F7372F36EB06BA08988E87C7BBC4">Reblova, Martina</mods:namePart>
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<mods:title id="B30E67C02B6F7B7F448108D635A305BC">MycoKeys</mods:title>
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<mods:date id="82EDFB8C36B5E5118579959E0B1F43B4">2019</mods:date>
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<mods:number id="9E06A2FFCA32B95628039928858AC5EC">55</mods:number>
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<mods:start id="B6F22990923E0162AEFAD9118971FF50">59</mods:start>
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<mods:url id="E31845BF861A8DDCF12B17F4A16CCD39">http://dx.doi.org/10.3897/mycokeys.55.35682</mods:url>
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<mods:classification id="D7EFD6C15DBE3C9B0372D21869CFF7CC">journal article</mods:classification>
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<mods:identifier id="AB8A9F89358E885648B04CFDEC725501" type="DOI">http://dx.doi.org/10.3897/mycokeys.55.35682</mods:identifier>
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<mods:identifier id="E2A438E5E61D1816D637AACEDDD24081" type="Pensoft-Pub">1314-4049-55-59</mods:identifier>
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<treatment id="8DC644221D9F7331E98EB4C9958E06E1" ID-GBIF-Taxon="158407448" LSID="urn:lsid:plazi:treatment:8DC644221D9F7331E98EB4C9958E06E1" httpUri="http://treatment.plazi.org/id/8DC644221D9F7331E98EB4C9958E06E1" lastPageNumber="59" pageId="0" pageNumber="59" scope_family="Aigialaceae" scope_order="Pleosporales">
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<subSubSection id="81C8D96F33FA5861A47D8C6C0D038198" pageId="0" pageNumber="59" type="nomenclature">
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<paragraph id="4B4A503BEED60105A2750D0ED8B991BB" pageId="0" pageNumber="59">
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<taxonomicName id="D2B70A48CAF15EE49FF8A0036D1E8B6E" ID-CoL="4M2TH" LSID="MB830267" authority="Vohnik & Reblova" class="Dothideomycetes" family="Aigialaceae" genus="Posidoniomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Posidoniomyces atricolor" order="Pleosporales" pageId="0" pageNumber="59" phylum="Ascomycota" rank="species" species="atricolor">
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Posidoniomyces atricolor
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<normalizedToken id="FEC68A411712C3287A5B55E84C64B68D" originalValue="Vohník">Vohnik</normalizedToken>
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&
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<normalizedToken id="94E8B2FE37EFB507848DA4D6E2B04A6B" originalValue="Réblová">Reblova</normalizedToken>
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</taxonomicName>
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<taxonomicNameLabel id="B82F6EC6C5205BAE999C07204224E838" pageId="0" pageNumber="59">sp. nov.</taxonomicNameLabel>
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Figs 5, 6
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</paragraph>
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</subSubSection>
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<subSubSection id="5BF47F29845982778FF37939920BFB40" pageId="0" pageNumber="59" type="typification">
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<paragraph id="3584E405519F3ECBF1473EF92C2E8A31" pageId="0" pageNumber="59">Typification.</paragraph>
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<paragraph id="71CBA7D2FFA4EE17B2487DC7BB922CD5" pageId="0" pageNumber="59">
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CROATIA. Dubrovnik-Neretva County: Potomje, Borak (
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<geoCoordinate id="38AC4A1F428958AC6DA6815FB352187D" direction="north" orientation="latitude" precision="1" value="42.92236">42.92236N</geoCoordinate>
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,
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<geoCoordinate id="9E8D6C7AC4B0A638AD966503D68AD6DC" direction="east" orientation="longitude" precision="1" value="17.34685">17.34685E</geoCoordinate>
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), isolated from a surface-sterilised healthy-looking terminal root of
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<taxonomicName id="64FE7C454645897184D7BC2BCB4DFED2" class="Liliopsida" family="Posidoniaceae" genus="Posidonia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Posidonia oceanica" order="Alismatales" pageId="0" pageNumber="59" phylum="Tracheophyta" rank="species" species="oceanica">Posidonia oceanica</taxonomicName>
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, 28 Sep 2016, M.
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<normalizedToken id="8241E84ADCF3203DB4FF135355FEC9C2" originalValue="Vohník">Vohnik</normalizedToken>
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& O.Borovec BRK-21 (holotype: PRA-15294!, dried culture - compact morphotype from a surface-sterilised root segment; isotype: PRA-15295!, dried culture - mycelial morphotype derived from the original compact colony).
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</paragraph>
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</subSubSection>
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<subSubSection id="61068F2E372F0C9F11B456ED5665CFBA" pageId="0" pageNumber="59" type="etymology">
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<paragraph id="2E7088DEB3816EE06C93E9C691D5A19C" pageId="0" pageNumber="59">Etymology.</paragraph>
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<paragraph id="B501E8B3C8860F69892CC2AD7A9F6123" pageId="0" pageNumber="59">
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<taxonomicName id="B4D3A1CAEDDBD3B67E77A7F29F2FBE70" genus="Atricolor" lsidName="Atricolor" pageId="0" pageNumber="59" rank="genus">Atricolor</taxonomicName>
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(L), meaning black, dark coloured, referring to the dark pigmented hyphae.
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</paragraph>
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</subSubSection>
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<subSubSection id="D3BF5791C02AC1888D6B59E2E5786BF9" pageId="0" pageNumber="59" type="description">
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<paragraph id="1CD8BB3207AD7EB00D83D25C7AFEB271" pageId="0" pageNumber="59">Description in culture.</paragraph>
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<paragraph id="E42FD5DC82603CE718EF8B215EB867CD" pageId="0" pageNumber="59">
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Mycelial colonial morphotype: Colonies on PCA 6-8 mm in diameter in 3 mo, circular, convex, appearing woolly, margin entire, aerial mycelium abundant, densest at the centre, cobwebby towards the margin, white to grey with a pale brown zone at the margin, colony surface with a dark brown hue formed by substrate mycelium and released pigment; reverse brown. Compact colonial morphotype: Colonies on PCA 5-6 mm in diameter in 8 mo, irregular, pulvinate, deeply furrowed, appearing mucoid-waxy to faintly floccose, of a
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<normalizedToken id="1D5CA99B3F1652342F1123937CC49C1D" originalValue="“cartilage”">"cartilage"</normalizedToken>
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consistency, become hollow upon aging, margin lobate, aerial mycelium scant, hyaline to pale brown, colony surface dark brown; reverse dark brown. Compact colonies, which are formed in vitro on sterilised roots of
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<taxonomicName id="B8A72D104EA34E65026FA08968854225" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
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, become irregular in shape, folded and furrowed in an almost cerebriform pattern, cacao brown, ca. 5-6 mm long on the longest side after several months of cultivation. Hyphae hyaline to pale brown, septate, smooth-walled and 2
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<normalizedToken id="9526A9984CED2C076F533F459E39286F" originalValue="–3(–">-3(-</normalizedToken>
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3.5)
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<normalizedToken id="BEB02288BB6EF2FCEABFAC855A82DFB7" originalValue="µm">µm</normalizedToken>
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wide, often with terminal, intercalary, rarely with lateral, one-celled, thick-walled globose, subglobose to ellipsoidal swellings 10-14
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<normalizedToken id="7A089A7184BB353233E404CF6C7D9492" originalValue="µm">µm</normalizedToken>
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wide; hyphae frequently protrude from these swellings and continue growing. Surface of the compact colonies covered by hyaline to subhyaline, smooth-walled hyphae with terminal, capitate swellings. Chlamydospores, conidiogenous cells or conidia, ascomatal initials and ascomata not observed.
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</paragraph>
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</subSubSection>
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<subSubSection id="FACEBBC6511ADCBCDBE78B0390EC161A" pageId="0" pageNumber="59" type="description">
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<paragraph id="035A5AAF68D44EA598FC5C6A5C594C6D" pageId="0" pageNumber="59">Description in vivo.</paragraph>
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<paragraph id="54112A9CB38D2DAB932F0383789B8D16" pageId="0" pageNumber="59">
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In vivo hyphae pigmented, septate, smooth-walled and (2
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<normalizedToken id="506036689C35B7F40DF290816B6ABD3C" originalValue="–)3–4(–">-)3-4(-</normalizedToken>
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5)
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<normalizedToken id="9CE17D702D70E401769C8C39DE4B207E" originalValue="µm">µm</normalizedToken>
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wide, colonising root cells of the host and/or forming an extraradical hyphal sheath, i.e. a finger-like pseudoparenchymatous net on the root surface.
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<taxonomicName id="B96A6912094E3BA2083C576C392BDB8E" genus="Microsclerotia" lsidName="Microsclerotia" pageId="0" pageNumber="59" rank="genus">Microsclerotia</taxonomicName>
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intracellular, melanised, round or elongated and 8
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<normalizedToken id="3A011FF980F2E6A91B7B4864FAF494D3" originalValue="–10(–">-10(-</normalizedToken>
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17)
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<normalizedToken id="242BFED5E9E4AF7DEF9031B0670AF229" originalValue="µm">µm</normalizedToken>
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wide, present in the
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<taxonomicName id="B759A1ABE81DB2E0C35F245578B2E062" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
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root hypodermis. Intracellular hyphae also infrequently occur in the rhizodermis.
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</paragraph>
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</subSubSection>
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<subSubSection id="2F6D444A5684AF258942AFD2E0283F07" pageId="0" pageNumber="59" type="materials_examined">
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<paragraph id="0195CCA7E608A4EAF12837A3E97EAACC" pageId="0" pageNumber="59">Specimens examined.</paragraph>
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<paragraph id="5A35F1662E30DFDFFE7EC57FC7D5F185" pageId="0" pageNumber="59">
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Croatia. Dubrovnik-Neretva County: Potomje, Borak (
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<geoCoordinate id="2BE4E530ED790323E6316A9FA6DDF36E" direction="north" orientation="latitude" precision="1" value="42.92236">42.92236N</geoCoordinate>
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,
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<geoCoordinate id="CCF3E1458673E5A781F65EC4545F7787" direction="east" orientation="longitude" precision="1" value="17.34685">17.34685E</geoCoordinate>
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), isolated from surface-sterilised healthy-looking terminal roots of
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<taxonomicName id="648934A0E6BFADE3F49F3B2D8669EB3F" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
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, 28 Sep 2016, M.
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<normalizedToken id="5362BE1EEE3B136D6188B65FA4E8FC8A" originalValue="Vohník">Vohnik</normalizedToken>
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& O.Borovec BRK-11 (PRA-15296); ibid., BRK-25 (PRA-15298); BRK-34 (PRA-15297); BRK-60 (PRA-15300); BRK-61 (PRA-15293); BRK-76 (PRA-15302); BRK-87 (PRA-15299); BRK-93 (PRA-15301), BRK-97 (PRA-15303). Croatia. Split-Dalmatia County:
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<normalizedToken id="806A19390EA71F3DEA036D44D4DA86A5" originalValue="Palagruža">Palagruza</normalizedToken>
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archipelago, Gangaro Island I (
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<geoCoordinate id="6197FCA9531569E2B2192E5AECA563D6" direction="north" orientation="latitude" precision="5" value="43.8639">43.8639N</geoCoordinate>
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,
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<geoCoordinate id="BFBD05B217401703109B7789190A682A" direction="east" orientation="longitude" precision="5" value="15.4341">15.4341E</geoCoordinate>
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), isolated from a surface-sterilised healthy-looking terminal root of
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<taxonomicName id="0BA362C108C1F416F6F2309A66470811" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
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, 3 September 2012, M.
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<normalizedToken id="BE6E890CA7423A60FADE2F6163878D40" originalValue="Vohník">Vohnik</normalizedToken>
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||
& O.Borovec M8. France.
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||
<normalizedToken id="122E0D41A20EFB3E988415287EB14C89" originalValue="Provence-Alpes-Côte">Provence-Alpes-Cote</normalizedToken>
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<normalizedToken id="6FB229C7F428CDD626448862692F904E" originalValue="d’Azur">d'Azur</normalizedToken>
|
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Region: Var Department,
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<normalizedToken id="C77E695F1ECBCDC72EA3DBB8072CC018" originalValue="Saint-Raphaël">Saint-Raphael</normalizedToken>
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||
, Cap Roux (
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||
<geoCoordinate id="8760D7B9838689DA406E724C3736490E" direction="north" orientation="latitude" precision="1" value="43.45026">43.45026N</geoCoordinate>
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||
,
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<geoCoordinate id="310C5DE5003525D0149661A8E4EB3457" direction="east" orientation="longitude" precision="1" value="6.91951">6.91951E</geoCoordinate>
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), isolated from a surface-sterilised healthy-looking terminal root of
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<taxonomicName id="9EBCBCCF484DBBAEDEE113670A50443A" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
, 17 June 2012, M.
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||
<normalizedToken id="0C4DF6612D1174BE30BC18E13BAF6827" originalValue="Vohník">Vohnik</normalizedToken>
|
||
P10. France.
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||
<normalizedToken id="5A4E1A96E77BFE47BB9EF676F061405C" originalValue="Provence-Alpes-Côte">Provence-Alpes-Cote</normalizedToken>
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||
<normalizedToken id="A8E5B73E3639A20260AF2665E099F1F9" originalValue="d’Azur">d'Azur</normalizedToken>
|
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Region: Alpes-Maritimes Department, Antibes (
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||
<geoCoordinate id="FFE40F0F90232D3A0752FE875816858C" direction="north" orientation="latitude" precision="1" value="43.55726">43.55726N</geoCoordinate>
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,
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<geoCoordinate id="C02973CA4444E21D0A478D341C24BB31" direction="east" orientation="longitude" precision="1" value="7.12209">7.12209E</geoCoordinate>
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||
), isolated from a surface-sterilised healthy-looking terminal root of
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||
<taxonomicName id="DDA91F7897FDDFD20F2AC0FBCEB18BB2" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
, 18 June 2012, M.
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||
<normalizedToken id="AFF2063AEB3C053B46176E0F80AF1D4F" originalValue="Vohník">Vohnik</normalizedToken>
|
||
P11. France.
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||
<normalizedToken id="E99835546D0384E9622639019A91604E" originalValue="Provence-Alpes-Côte">Provence-Alpes-Cote</normalizedToken>
|
||
<normalizedToken id="D98DACB4FB39390DA8C6975C78965EF7" originalValue="d’Azur">d'Azur</normalizedToken>
|
||
Region: Var Department, Sanary-sur-Mer (
|
||
<geoCoordinate id="7B4B879EF901904CF8AE8E7E0D9A9E03" direction="north" orientation="latitude" precision="1" value="43.12054">43.12054N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="8A77064F564EA1EB348A3D7EACF0DB08" direction="east" orientation="longitude" precision="1" value="5.77545">5.77545E</geoCoordinate>
|
||
), isolated from a surface-sterilised healthy-looking terminal root of
|
||
<taxonomicName id="5C542E3A8382F91614EB11101B786BE9" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
, 19 June 2012, M.
|
||
<normalizedToken id="02EA116E8609D278B3F2D8D7B7A20EB2" originalValue="Vohník">Vohnik</normalizedToken>
|
||
P15. Italy. Liguria Region: Savona Province, Gulf of Genoa, Finale Ligure (
|
||
<geoCoordinate id="B0AFF6604B8C1F5D37DE4B0021B5918A" direction="north" orientation="latitude" precision="1" value="44.17337">44.17337N</geoCoordinate>
|
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,
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||
<geoCoordinate id="256301A9802B3B8A22EA879386F095FC" direction="east" orientation="longitude" precision="1" value="8.36765">8.36765E</geoCoordinate>
|
||
), isolated from a surface-sterilised healthy-looking terminal root of
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||
<taxonomicName id="50FB6BA1A1E6B9C3FDDDB5F9DED2B80B" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
, 17 June 2012, M.
|
||
<normalizedToken id="358355415142B3F6B02835671982C58C" originalValue="Vohník">Vohnik</normalizedToken>
|
||
P09. Spain. Girona Province:
|
||
<normalizedToken id="1BBD646EDC34E7D895E93495F090CE52" originalValue="L’Escala">L'Escala</normalizedToken>
|
||
(
|
||
<geoCoordinate id="B88E6D187211CD99906A6D3C59CB6AB8" direction="north" orientation="latitude" precision="1" value="42.10744">42.10744N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="7958C0963AC3D8FE38D7B9C6237E2FA6" direction="east" orientation="longitude" precision="1" value="3.16892">3.16892E</geoCoordinate>
|
||
), isolated from a surface-sterilised healthy-looking terminal root of
|
||
<taxonomicName id="A270918647CD8A15EE955CDE474D6D3D" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
, 18 June 2012, M.
|
||
<normalizedToken id="31EE42B9C75BD2E5F934C467FDA08298" originalValue="Vohník">Vohnik</normalizedToken>
|
||
P20.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="29C6F5A8403778679863EB7DF2A1BE7F" pageId="0" pageNumber="59" type="habitat">
|
||
<paragraph id="365A295179B16479F8C50DFCBE18D6E0" pageId="0" pageNumber="59">Habitat and distribution.</paragraph>
|
||
<paragraph id="4189453AC08E85D21DA94495461D3F2F" pageId="0" pageNumber="59">
|
||
Root mycobiont of the dominant and endemic Mediterranean seagrass
|
||
<taxonomicName id="505E97061BABC9D055F0087D98C86529" class="Liliopsida" family="Posidoniaceae" genus="Posidonia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Posidonia oceanica" order="Alismatales" pageId="0" pageNumber="59" phylum="Tracheophyta" rank="species" species="oceanica">Posidonia oceanica</taxonomicName>
|
||
. So far known only from the NW Mediterranean Sea.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="4B1DEDB9E0224D65CD3A5CFACDBD952D" pageId="0" pageNumber="59" type="notes">
|
||
<paragraph id="E1D18B430204B3EE74AFD9C6079AE45C" pageId="0" pageNumber="59">Notes.</paragraph>
|
||
<paragraph id="4D9CA52A0CAE1CE7396FBCAB299BDC84" pageId="0" pageNumber="59">
|
||
Both colonial morphotypes, named compact and mycelial, appeared on surface-sterilised root segments of
|
||
<taxonomicName id="4BCA33827015E5A3075D78D13DF91598" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
and after inoculation also on solid agar media but the compact colonies with the cerebriform pattern formed only on the original root segments. All examined colonies of
|
||
<taxonomicName id="DAA7E6418B9B30E626EC1E4C52B84FA0" lsidName="P. atricolor" pageId="0" pageNumber="59" rank="species" species="atricolor">P. atricolor</taxonomicName>
|
||
emerging from the original root segments developed from melanised microsclerotia formed exclusively intracellularly in the
|
||
<taxonomicName id="D5E63CE571AF48A41CB0CCCED6E308F0" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
hypodermis (Figure 5d, h). The mycelial morphotype was observed on MMN and PCA, while compact colonies were formed on PDA and PCA (
|
||
<bibRefCitation id="9A0349EED49502673DC7A583AF99943F" author="Vohnik, M" journalOrPublisher="Microbial Ecology" pageId="0" pageNumber="59" pagination="442 - 451" title="Communities of cultivable root mycobionts of the seagrass Posidoniaoceanica in the northwest Mediterranean Sea are dominated by a hitherto undescribed pleosporalean dark septate endophyte." url="https://doi.org/10.1007/s00248-015-0640-5" volume="71" year="2016">
|
||
<normalizedToken id="903FA88EB0C8C5B1F80F30FDD9272B9E" originalValue="Vohník">Vohnik</normalizedToken>
|
||
et al. 2016
|
||
</bibRefCitation>
|
||
; this study). When the surface of a colony exhibiting the compact colonial morphotype was washed regularly with sterile tap water, fragments of hyphae were released to form minute daughter colonies (Figure 6e). These daughter colonies were either of a rhizoidal form composed of substrate mycelium and continued to develop the mycelial morphotype or they assumed the compact colony character from the beginning (Figure 6f). A new hypha was often formed through the globose swelling, regardless of its position on the hypha (Figures 6
|
||
<normalizedToken id="300E714A404FCD58110A6DC740B12B1B" originalValue="i–k">i-k</normalizedToken>
|
||
).
|
||
</paragraph>
|
||
<caption id="7B08E49C7C4975B834C884CC7A4924F1" pageId="0" pageNumber="59">
|
||
<paragraph id="8A9D14702EC47477C3AE519B3207C6AF" pageId="0" pageNumber="59">
|
||
Figure 5. In vivo root colonisation pattern and in vitro cultural aspects of
|
||
<taxonomicName id="1608C66914655D96DE2D0519FB658329" class="Dothideomycetes" family="Aigialaceae" genus="Posidoniomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Posidoniomyces atricolor" order="Pleosporales" pageId="0" pageNumber="59" phylum="Ascomycota" rank="species" species="atricolor">Posidoniomyces atricolor</taxonomicName>
|
||
. a In vivo colonisation on the root surface (arrows) and in the hypodermis (asterisks) of
|
||
<taxonomicName id="CEACC073F2B808F652A36D742B0C244A" lsidName="P. oceanica" pageId="0" pageNumber="59" rank="species" species="oceanica">P. oceanica</taxonomicName>
|
||
bDSE colonisation on the root surface c germinating microsclerotia stained with trypan blue (arrows) d compact colony developed from microsclerotia (arrow) e surface-sterilised root segments yielding
|
||
<taxonomicName id="135EA841327F2AF4FCFD9A6FE70E589D" lsidName="P. atricolor" pageId="0" pageNumber="59" rank="species" species="atricolor">P. atricolor</taxonomicName>
|
||
compact colonies (black arrows), sometimes with substrate mycelium (white arrows) f compact colonial morphotype g mycelial colonial morphotype h mycelial morphotype developing from microsclerotia (arrows) in transversal section. Scale bars: 20
|
||
<normalizedToken id="95DE6BA4DC796495EACE6165E1912CED" originalValue="μm">μm</normalizedToken>
|
||
(a, b), 50
|
||
<normalizedToken id="08BC5D41C13FE64855A9252779DC62AA" originalValue="μm">μm</normalizedToken>
|
||
(c), 100
|
||
<normalizedToken id="C0394A64717EE2581025693668A50A1B" originalValue="μm">μm</normalizedToken>
|
||
(d), 200
|
||
<normalizedToken id="B1D952C8BC3AB602179E6E5B067CBF93" originalValue="μm">μm</normalizedToken>
|
||
(f, h), 500
|
||
<normalizedToken id="E712B49DCE9F087C8DDB46715F385D30" originalValue="μm">μm</normalizedToken>
|
||
(g).
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="E83076AAD6F5D7B745D6DC9BD4BA8B22" pageId="0" pageNumber="59">
|
||
<paragraph id="730CA4D5BF754BC51B99E9D8B41E8547" pageId="0" pageNumber="59">
|
||
Figure 6. Colonial morphotypes of
|
||
<taxonomicName id="A0130689250D8AF39346E161871D8E46" class="Dothideomycetes" family="Aigialaceae" genus="Posidoniomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Posidoniomyces atricolor" order="Pleosporales" pageId="0" pageNumber="59" phylum="Ascomycota" rank="species" species="atricolor">Posidoniomyces atricolor</taxonomicName>
|
||
in vitro (type isolate BRK-21). a Compact morphotype with substrate mycelium b, d compact colonies with a cerebriform pattern c colony of
|
||
<taxonomicName id="5922A7A82CAB8F87151A8604B9813593" lsidName="P. atricolor" pageId="0" pageNumber="59" rank="species" species="atricolor">P. atricolor</taxonomicName>
|
||
on PCA e rhizoidal and compact (arrow) daughter colonies on PCA washed with sterile tap water f detail of the colonies encircled in e; g, h terminal capitate swellings on the surface of compact colonies
|
||
<normalizedToken id="84C4C6A80BC90D107CB54C7225F967FF" originalValue="i–k">i-k</normalizedToken>
|
||
conspicuous swellings on aerial mycelium. Scale bars: 500
|
||
<normalizedToken id="ED40FA84A34CAB8B00B10532FC764C00" originalValue="μm">μm</normalizedToken>
|
||
(a, d), 1000
|
||
<normalizedToken id="A5CA380D1F85F018A3AFD9B56692E814" originalValue="μm">μm</normalizedToken>
|
||
(b, c), 5 mm (e), 200
|
||
<normalizedToken id="E8A322EC64AC637CF0925C2F032B2ACB" originalValue="μm">μm</normalizedToken>
|
||
(f), 100
|
||
<normalizedToken id="0C981B47D9A72CBB88F208A7E335C116" originalValue="μm">μm</normalizedToken>
|
||
(g), 20
|
||
<normalizedToken id="2B120A91B3AD1D1845CAB23C181917E1" originalValue="μm">μm</normalizedToken>
|
||
(h).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |