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<document id="ABC34FE7B9601B8C723FE0EECA2FB513" ID-CLB-Dataset="30029" ID-DOI="10.11646/zootaxa.4422.4.4" ID-GBIF-Dataset="3fb6bb16-d9ff-4ca3-a474-86c4d1abdc95" ID-ISSN="1175-5326" ID-Zenodo-Dep="1253240" ID-ZooBank="50FBD8F3-327B-4562-B587-66180E329DB3" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1527266783242" checkinUser="plazi" docAuthor="Fortaleza, Maria Tarciana Vieira &amp; Lotufo, Tito Monteiro Da Cruz" docDate="2018" docId="038D3D37FF9FFF8CC0E572A068D6FBC8" docLanguage="en" docName="zootaxa.4422.4.4.pdf.imf" docOrigin="Zootaxa 4422 (4)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Aplidium hourglassum Fortaleza &amp; Lotufo 2018, sp. n." docType="treatment" docUuid="5E0EFFCA-2B80-4156-BAEA-3B392CC5E22A" docUuidSource="ZooBank" docVersion="9" lastPageNumber="527" masterDocId="FFB4454FFF98FF84C07272376B61FFD8" masterDocTitle="Polyclinidae (Ascidiacea: Aplousobranchia) from the Gulf of Mexico, collected during the “ Hourglass ” cruises (1965 - 1967)" masterLastPageNumber="536" masterPageNumber="519" pageNumber="526" updateTime="1698545428781" updateUser="ExternalLinkService">
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<mods:title id="823EF52C441CE6664DCAA64DB4005ECD">Polyclinidae (Ascidiacea: Aplousobranchia) from the Gulf of Mexico, collected during the “ Hourglass ” cruises (1965 - 1967)</mods:title>
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<mods:namePart id="BB5B89F7BBB906A4E6D0C2CC6FBAAD6E">Fortaleza, Maria Tarciana Vieira</mods:namePart>
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<mods:namePart id="125DE39D38EEDC145F704559E96DB371">Lotufo, Tito Monteiro Da Cruz</mods:namePart>
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<treatment id="038D3D37FF9FFF8CC0E572A068D6FBC8" ID-DOI="http://doi.org/10.5281/zenodo.5959096" ID-GBIF-Taxon="144128941" ID-Zenodo-Dep="5959096" LSID="urn:lsid:zoobank.org:act:5E0EFFCA-2B80-4156-BAEA-3B392CC5E22A" httpUri="http://treatment.plazi.org/id/038D3D37FF9FFF8CC0E572A068D6FBC8" lastPageId="8" lastPageNumber="527" pageId="7" pageNumber="526">
<subSubSection id="C33EDFAAFF9FFF83C0E572A06A4EFF0B" pageId="7" pageNumber="526" type="nomenclature">
<paragraph id="8B9B8C21FF9FFF83C0E572A06A97FF6A" blockId="7.[151,502,151,211]" box="[151,502,151,178]" pageId="7" pageNumber="526">
<heading id="D0D33B4DFF9FFF83C0E572A06A97FF6A" bold="true" box="[151,502,151,178]" fontSize="11" level="1" pageId="7" pageNumber="526" reason="1">
<emphasis id="B9505033FF9FFF83C0E572A06A97FF6A" bold="true" box="[151,502,151,178]" pageId="7" pageNumber="526">
<taxonomicName id="4C24F7A2FF9FFF83C0E572A06ACDFF69" authority="Fortaleza &amp; Lotufo, 2018" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[151,428,151,177]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="7" pageNumber="526" phylum="Chordata" rank="species" species="hourglassum" status="sp. nov.">
<emphasis id="B9505033FF9FFF83C0E572A06ACDFF69" bold="true" box="[151,428,151,177]" italics="true" pageId="7" pageNumber="526">Aplidium hourglassum</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A263ED48FF9FFF83C1C672AF6A97FF6A" box="[436,502,152,178]" pageId="7" pageNumber="526" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
<paragraph id="8B9B8C21FF9FFF83C0E5728D6A4EFF0B" blockId="7.[151,502,151,211]" box="[151,303,186,211]" pageId="7" pageNumber="526">
(
<figureCitation id="131F90A4FF9FFF83C0EC728D6A6BFF0B" box="[158,266,186,211]" captionStart="FIGURE 8" captionStartId="7.[151,250,1223,1245]" captionText="FIGURE 8. Aplidium hourglassum sp. n. Scale: 1 cm—Material preserved in formalin." pageId="7" pageNumber="526">Figures 8</figureCitation>
,
<figureCitation id="131F90A4FF9FFF83C165728C6A47FF0B" box="[279,294,187,211]" captionStart="FIGURE 9" captionStartId="7.[151,250,1936,1958]" captionTargetBox="[345,1247,1297,1905]" captionTargetId="figure@7.[340,1247,1297,1911]" captionTargetPageId="7" captionText="FIGURE 9. Aplidium hourglassum sp. n. A—zooid; B and C—larvae. Scale: A = 1 mm; B and C = 0,1 mm." httpUri="https://zenodo.org/record/1253250/files/figure.png" pageId="7" pageNumber="526">9</figureCitation>
)
</paragraph>
</subSubSection>
<subSubSection id="C33EDFAAFF9FFF83C0E57335685AFEC3" box="[151,827,258,283]" pageId="7" pageNumber="526" type="etymology">
<paragraph id="8B9B8C21FF9FFF83C0E57335685AFEC3" blockId="7.[151,1437,258,463]" box="[151,827,258,283]" pageId="7" pageNumber="526">
<emphasis id="B9505033FF9FFF83C0E573356A40FEC3" bold="true" box="[151,289,258,283]" pageId="7" pageNumber="526">Etymology:</emphasis>
Species name refers to the “Hourglass” cruises.
</paragraph>
</subSubSection>
<subSubSection id="C33EDFAAFF9FFF83C0B573116E15FEE7" box="[199,1396,294,319]" pageId="7" pageNumber="526" type="materials_examined">
<paragraph id="8B9B8C21FF9FFF83C0B573116E15FEE7" blockId="7.[151,1437,258,463]" box="[199,1396,294,319]" pageId="7" pageNumber="526">
<emphasis id="B9505033FF9FFF83C0B573116AD4FEE7" bold="true" box="[199,437,294,319]" pageId="7" pageNumber="526">Examined material:</emphasis>
Holotype: collected in
<date id="FF9AAAE1FF9FFF83C2CF73116842FEE7" box="[701,803,294,319]" pageId="7" pageNumber="526" value="1966-06-07">7/6/1966</date>
, 27°37N, 084°13W,
<quantity id="4CDC21C4FF9FFF83C46F73106F0CFEE6" box="[1053,1133,295,319]" metricMagnitude="1" metricUnit="m" metricValue="7.32" pageId="7" pageNumber="526" unit="m" value="73.2">73.2 m</quantity>
, specimen #EJ-66-222.
</paragraph>
</subSubSection>
<subSubSection id="C33EDFAAFF9FFF8CC0B5737D68CBFD5D" lastPageId="8" lastPageNumber="527" pageId="7" pageNumber="526" type="description">
<paragraph id="8B9B8C21FF9FFF83C0B5737D683EFE17" blockId="7.[151,1437,258,463]" pageId="7" pageNumber="526">
<emphasis id="B9505033FF9FFF83C0B5737D6A3AFEBB" bold="true" box="[199,347,330,355]" pageId="7" pageNumber="526">Description:</emphasis>
Colony of irregular format, measuring
<quantity id="4CDC21C4FF9FFF83C352737C680FFEBA" box="[800,878,331,355]" metricMagnitude="-2" metricUnit="m" metricValue="1.8" pageId="7" pageNumber="526" unit="cm" value="1.8">1.8 cm</quantity>
x
<quantity id="4CDC21C4FF9FFF83C3FC737C68BFFEBA" box="[910,990,331,355]" metricMagnitude="-3" metricUnit="m" metricValue="4.0" pageId="7" pageNumber="526" unit="cm" value="0.4">0.4 cm</quantity>
, attached to the substrate by the whole basal portion. Colony surface smooth and without incrustations. Small amounts of foreign material in the matrix and base of the colony. Tunic soft, transparent and yellowish, zooids with a darker brownish coloration. Zooids not organized in systems. Presence of copepods in the tunic matrix.
</paragraph>
<caption id="DF5BDCA9FF9FFF83C0E576F06F6CFB05" box="[151,1037,1223,1245]" pageId="7" pageNumber="526">
<paragraph id="8B9B8C21FF9FFF83C0E576F06F6CFB05" blockId="7.[151,1037,1223,1245]" box="[151,1037,1223,1245]" pageId="7" pageNumber="526">
<emphasis id="B9505033FF9FFF83C0E576F06A73FB04" bold="true" box="[151,274,1223,1245]" pageId="7" pageNumber="526">FIGURE 8.</emphasis>
<taxonomicName id="4C24F7A2FF9FFF83C16B76F06A9FFB05" authority="Fortaleza &amp; Lotufo, 2018" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[281,510,1223,1245]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="7" pageNumber="526" phylum="Chordata" rank="species" species="hourglassum" status="sp. nov.">
<emphasis id="B9505033FF9FFF83C16B76F06A9FFB05" box="[281,510,1223,1245]" italics="true" pageId="7" pageNumber="526">Aplidium hourglassum</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A263ED48FF9FFF83C27676FF695AFB05" box="[516,571,1224,1245]" pageId="7" pageNumber="526" rank="species">sp. n.</taxonomicNameLabel>
Scale: 1 cm—Material preserved in formalin.
</paragraph>
</caption>
<caption id="DF5BDCA9FF9FFF83C0E575A76F85F87E" box="[151,1252,1936,1958]" httpUri="https://zenodo.org/record/1253250/files/figure.png" pageId="7" pageNumber="526" startId="7.[151,250,1936,1958]" targetBox="[345,1247,1297,1905]" targetPageId="7">
<paragraph id="8B9B8C21FF9FFF83C0E575A76F85F87E" blockId="7.[151,1252,1936,1958]" box="[151,1252,1936,1958]" pageId="7" pageNumber="526">
<emphasis id="B9505033FF9FFF83C0E575A76A73F87D" bold="true" box="[151,274,1936,1958]" pageId="7" pageNumber="526">FIGURE 9.</emphasis>
<taxonomicName id="4C24F7A2FF9FFF83C16B75A76A9FF87E" authority="Fortaleza &amp; Lotufo, 2018" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[281,510,1936,1958]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="7" pageNumber="526" phylum="Chordata" rank="species" species="hourglassum" status="sp. nov.">
<emphasis id="B9505033FF9FFF83C16B75A76A9FF87E" box="[281,510,1936,1958]" italics="true" pageId="7" pageNumber="526">Aplidium hourglassum</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A263ED48FF9FFF83C27675A6695AF87E" box="[516,571,1937,1958]" pageId="7" pageNumber="526" rank="species">sp. n.</taxonomicNameLabel>
A—zooid; B and C—larvae. Scale: A = 1 mm; B and C = 0,1 mm.
</paragraph>
</caption>
<paragraph id="8B9B8C21FF90FF8CC0B572A06F7AFDC0" blockId="8.[151,1437,151,1040]" pageId="8" pageNumber="527">
The zooids may vary between
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in length depending on the contraction state (length rangethorax and abdomen =
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; post-abdomen =
<quantity id="4CDC21C4FF90FF8CC2C7728B683DFF0C" box="[693,860,188,212]" metricMagnitude="-4" metricUnit="m" metricValue="5.6" metricValueMax="8.0" metricValueMin="3.2" pageId="8" pageNumber="527" unit="mm" value="0.56" valueMax="0.8" valueMin="0.32">0.320.80 mm</quantity>
). Branchial siphon very short, measuring
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and exhibiting six rounded lobes. Atrial opening short and smooth edged, located beside the second stigmata row. Atrial languet simple and very small, maximum length of
<quantity id="4CDC21C4FF90FF8CC3FE73336899FEC4" box="[908,1016,260,284]" metricMagnitude="-4" metricUnit="m" metricValue="2.0" pageId="8" pageNumber="527" unit="mm" value="0.2">0.20 mm</quantity>
. Muscles are strong, with 10 to 15 longitudinal bundles in each side of the body running in parallel along the zooid. Pharynx with 10 rows of stigmata. The number of stigmata per row could not be counted. Esophagus short. Stomach slightly squared and flattened in the anterior and posterior ends, with 25 to 30 well-defined continuous longitudinal folds. The size of the esophagus and stomach together are equal to slightly more than half of the abdomen length. Posterior stomach discernible in some zooids. Anal opening located beside the fifth row of stigmata. Abdomen and post-abdomen not separated by a constriction. Gonads developed in some zooids; however, in most zooids, gonads are poorly developed, around 15 testicular follicles organized in a double row together with six yellow ovules.
</paragraph>
<paragraph id="8B9B8C21FF90FF8CC0B5701268CBFD5D" blockId="8.[151,1437,151,1040]" pageId="8" pageNumber="527">
Up to three embryos and/or larvae in a single series in the posterior thorax region. Larvae up to
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long, 11 to 16 adhesive papillae with long and thin peduncles, ampullae absent, several small epidermal vesicles distributed over the whole larva; tail wound halfway around the trunk.
</paragraph>
</subSubSection>
<subSubSection id="C33EDFAAFF90FF8CC0B570B868D6FBC8" pageId="8" pageNumber="527" type="discussion">
<paragraph id="8B9B8C21FF90FF8CC0B570B868D6FBC8" blockId="8.[151,1437,151,1040]" pageId="8" pageNumber="527">
<emphasis id="B9505033FF90FF8CC0B570B86A55FD70" bold="true" box="[199,308,655,680]" pageId="8" pageNumber="527">Remarks</emphasis>
.
<taxonomicName id="4C24F7A2FF90FF8CC13370A7695EFD70" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[321,575,656,680]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="hourglassum">
<emphasis id="B9505033FF90FF8CC13370A7695EFD70" box="[321,575,656,680]" italics="true" pageId="8" pageNumber="527">Aplidium hourglassum</emphasis>
</taxonomicName>
is distinguished by the combination of a small number of rows of stigmata, a large number of stomach folds, and numerous adhesive papillae in the large larvae. The genus
<taxonomicName id="4C24F7A2FF90FF8CC4A670836E5BFD14" box="[1236,1338,692,716]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="genus">
<emphasis id="B9505033FF90FF8CC4A670836E5BFD14" box="[1236,1338,692,716]" italics="true" pageId="8" pageNumber="527">Aplidium</emphasis>
</taxonomicName>
is fairly diverse, comprising about 280 valid species (WoRMS, accessed
<date id="FF9AAAE1FF90FF8CC30270E06F31FD28" box="[880,1104,727,752]" pageId="8" pageNumber="527" value="2017-09-26">26 September 2017</date>
). According to Kotts review, only
<taxonomicName id="4C24F7A2FF90FF8CC0A170CB6963FCCD" authority="(Kott, 1963)" baseAuthorityName="Kott" baseAuthorityYear="1963" box="[211,514,764,789]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="triggsense">
<emphasis id="B9505033FF90FF8CC0A170CB6A08FCCC" box="[211,361,764,788]" italics="true" pageId="8" pageNumber="527">A. triggsense</emphasis>
(
<bibRefCitation id="EFB5F1D0FF90FF8CC10970CB6A98FCCD" box="[379,505,764,789]" pageId="8" pageNumber="527" refId="ref6107" refString="Kott, P. (1963) The Ascidians of Australia. Australian Journal of Marine &amp; Freshwater Research, 14, 70 - 118. https: // doi. org / 10.1071 / MF 9630070" type="journal article">Kott, 1963</bibRefCitation>
)
</taxonomicName>
and
<taxonomicName id="4C24F7A2FF90FF8CC24D70CB68A1FCCD" authority="(Millar, 1975)" baseAuthorityName="Millar" baseAuthorityYear="1975" box="[575,960,764,789]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="multipapillatum">
<emphasis id="B9505033FF90FF8CC24D70CB6874FCCC" box="[575,789,764,788]" italics="true" pageId="8" pageNumber="527">A. multipapillatum</emphasis>
(
<bibRefCitation id="EFB5F1D0FF90FF8CC35570CB68D9FCCD" box="[807,952,764,789]" pageId="8" pageNumber="527" refId="ref6499" refString="Millar, R. H. (1975) Ascidians from the Indo-West-Pacific region in the Zoological Museum, Copenhagen (Tunicata, Ascidiacea). Steenstrupia, 3, 205 - 336." type="journal article">Millar, 1975</bibRefCitation>
)
</taxonomicName>
have an uncommon number of adhesive papillae in the larvae (
<bibRefCitation id="EFB5F1D0FF90FF8CC1E671176973FCE0" box="[404,530,800,824]" pageId="8" pageNumber="527" refId="ref6144" refString="Kott, P. (1992) The Australian Ascidiacea part 3, Aplousobranchia (2). Memoirs of the Queensland Museum, 32, 375 - 620." type="journal article">Kott, 1992</bibRefCitation>
). The
<taxonomicName id="4C24F7A2FF90FF8CC2287117681DFCE0" baseAuthorityName="Millar" baseAuthorityYear="1975" box="[602,892,800,824]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="multipapillatum">
<emphasis id="B9505033FF90FF8CC2287117681DFCE0" box="[602,892,800,824]" italics="true" pageId="8" pageNumber="527">Aplidium multipapillatum</emphasis>
</taxonomicName>
, common in the
<collectingCountry id="F333CCB1FF90FF8CC44F71286FE1FCE0" box="[1085,1152,799,824]" name="China" pageId="8" pageNumber="527">China</collectingCountry>
Sea, has only four to six papillae, which is much lower than the number found in
<taxonomicName id="4C24F7A2FF90FF8CC35F717368BEFC84" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[813,991,836,860]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="hourglassum">
<emphasis id="B9505033FF90FF8CC35F717368BEFC84" box="[813,991,836,860]" italics="true" pageId="8" pageNumber="527">A. hourglassum</emphasis>
</taxonomicName>
. (
<bibRefCitation id="EFB5F1D0FF90FF8CC38571736FEAFC85" box="[1015,1163,836,861]" pageId="8" pageNumber="527" refId="ref6499" refString="Millar, R. H. (1975) Ascidians from the Indo-West-Pacific region in the Zoological Museum, Copenhagen (Tunicata, Ascidiacea). Steenstrupia, 3, 205 - 336." type="journal article">Millar, 1975</bibRefCitation>
). In contrast, the larvae from
<taxonomicName id="4C24F7A2FF90FF8CC0AB715F6A11FCA7" baseAuthorityName="Kott" baseAuthorityYear="1963" box="[217,368,872,895]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="triggsense">
<emphasis id="B9505033FF90FF8CC0AB715F6A11FCA7" box="[217,368,872,895]" italics="true" pageId="8" pageNumber="527">A. triggsense</emphasis>
</taxonomicName>
, a common species in the waters of
<collectingRegion id="49E042C3FF90FF8CC35E715F6897FC58" box="[812,1014,871,896]" country="Australia" name="Western Australia" pageId="8" pageNumber="527">
western
<collectingCountry id="F333CCB1FF90FF8CC3FD71506897FC58" box="[911,1014,871,896]" name="Australia" pageId="8" pageNumber="527">Australia</collectingCountry>
</collectingRegion>
and
<collectingCountry id="F333CCB1FF90FF8CC444715F6F8AFC58" box="[1078,1259,871,896]" name="New Caledonia" pageId="8" pageNumber="527">New Caledonia</collectingCountry>
, have seven to fourteen adhesive papillae, but are considerably smaller, measuring only
<quantity id="4CDC21C4FF90FF8CC3A171BB6F5AFC7C" box="[979,1083,908,933]" metricMagnitude="-4" metricUnit="m" metricValue="6.0" pageId="8" pageNumber="527" unit="mm" value="0.6">0.60 mm</quantity>
, and a zooid with just 15 to 16 longitudinal folds in the stomach (
<bibRefCitation id="EFB5F1D0FF90FF8CC254718769C4FC10" box="[550,677,943,968]" pageId="8" pageNumber="527" refId="ref6107" refString="Kott, P. (1963) The Ascidians of Australia. Australian Journal of Marine &amp; Freshwater Research, 14, 70 - 118. https: // doi. org / 10.1071 / MF 9630070" type="journal article">Kott, 1963</bibRefCitation>
;
<bibRefCitation id="EFB5F1D0FF90FF8CC2C671876852FC10" box="[692,819,944,968]" pageId="8" pageNumber="527" refId="ref6144" refString="Kott, P. (1992) The Australian Ascidiacea part 3, Aplousobranchia (2). Memoirs of the Queensland Museum, 32, 375 - 620." type="journal article">Kott, 1992</bibRefCitation>
). Hence, both differ from
<taxonomicName id="4C24F7A2FF90FF8CC41771876E76FC10" authorityName="Fortaleza &amp; Lotufo" authorityYear="2018" box="[1125,1303,944,968]" class="Ascidiacea" family="Polyclinidae" genus="Aplidium" higherTaxonomySource="GBIF" kingdom="Animalia" order="Enterogona" pageId="8" pageNumber="527" phylum="Chordata" rank="species" species="hourglassum">
<emphasis id="B9505033FF90FF8CC41771876E76FC10" box="[1125,1303,944,968]" italics="true" pageId="8" pageNumber="527">A. hourglassum</emphasis>
</taxonomicName>
, which has larvae up to
<quantity id="4CDC21C4FF90FF8CC15B71E36AF1FC34" box="[297,400,980,1004]" metricMagnitude="-3" metricUnit="m" metricValue="1.04" pageId="8" pageNumber="527" unit="mm" value="1.04">1.04 mm</quantity>
long, 1416 adhesive papillae, and 2530 stomach folds. After Kotts review in 1992, no other species with this number of papillae was described for this genus.
</paragraph>
</subSubSection>
</treatment>
</document>