181 lines
12 KiB
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181 lines
12 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.65.47704" ID-GBIF-Dataset="00685a01-0785-47ec-aa0b-77c991ee4b08" ID-PMC="PMC7078340" ID-Pensoft-Pub="1314-4049-65-49" ID-Pensoft-UUID="DED2BF86E6FD56179642F7119604CEB9" ID-PubMed="32206025" ModsDocID="1314-4049-65-49" checkinTime="1583860301782" checkinUser="pensoft" docAuthor="Hou, Lingwei, Hernandez-Restrepo, Margarita, Groenewald, Johannes Zacharias, Cai, Lei & Crous, Pedro W." docDate="2020" docId="667CE759730C5AB78C087077CFF0CB63" docLanguage="en" docName="MycoKeys 65: 49-99" docOrigin="MycoKeys 65" docSource="http://dx.doi.org/10.3897/mycokeys.65.47704" docTitle="Didymella kooimaniorum Hern. - Restr., L. W. Hou, L. Cai & Crous 2020, sp. nov." docType="treatment" docVersion="4" id="DED2BF86E6FD56179642F7119604CEB9" lastPageNumber="49" masterDocId="DED2BF86E6FD56179642F7119604CEB9" masterDocTitle="Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes)" masterLastPageNumber="99" masterPageNumber="49" pageNumber="49" updateTime="1668136425574" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes)</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hou, Lingwei</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hernandez-Restrepo, Margarita</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Groenewald, Johannes Zacharias</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Cai, Lei</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Crous, Pedro W.</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2020</mods:date>
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<mods:detail type="volume">
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<mods:number>65</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>49</mods:start>
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<mods:end>99</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.65.47704</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.65.47704</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-65-49</mods:identifier>
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<mods:identifier type="Pensoft-UUID">DED2BF86E6FD56179642F7119604CEB9</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="162481725" LSID="urn:lsid:plazi:treatment:667CE759730C5AB78C087077CFF0CB63" httpUri="http://treatment.plazi.org/id/667CE759730C5AB78C087077CFF0CB63" lastPageNumber="49" pageId="0" pageNumber="49">
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<subSubSection pageId="0" pageNumber="49" type="nomenclature">
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName LSID="667CE759-730C-5AB7-8C08-7077CFF0CB63" authority="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Didymella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Didymella kooimaniorum" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="kooimaniorum" status="sp. nov.">Didymella kooimaniorum Hern.-Restr., L. W. Hou, L. Cai & Crous</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="49">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Didymella kooimaniorum (CBS 144951). A, B Colony on OA (front and reverse) C, D colony on MEA (front and reverse) E, F colony on PDA (front and reverse) G pycnidia forming on OA H pycnidia I section of pycnidium J section of pycnidial wall K-M conidiogenous cells N conidia. Scale bars: 100 μm (H); 50 μm (I); 10 μm (J); 5 μm (K-N)." figureDoi="10.3897/mycokeys.65.47704.figure4" httpUri="https://binary.pensoft.net/fig/388207" pageId="0" pageNumber="49">Figure 4</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="etymology">
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<paragraph pageId="0" pageNumber="49">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Didymella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="kooimaniorum" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="kooimaniorum">
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<emphasis italics="true" pageId="0" pageNumber="49">kooimaniorum</emphasis>
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</taxonomicName>
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refers to Noud & Robin Kooiman who collected the soil sample from which the ex-type strain was isolated.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="typus">
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<paragraph pageId="0" pageNumber="49">Typus.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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The Netherlands. Utrecht province, Vleuten, isolated from garden soil, Mar. 2017, N. Kooiman & R. Kooiman (
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<emphasis bold="true" italics="true" pageId="0" pageNumber="49">holotype</emphasis>
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designated here CBS H-24106, living ex-type culture CBS 144951 = JW 27006).
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</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<emphasis italics="true" pageId="0" pageNumber="49">Conidiomata</emphasis>
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pycnidial, superficial or semi-immersed, scattered or solitary, sometimes confluent, globose to subglobose, irregularly-shaped with age, pale brown to brown, covered by hyphal outgrowths, especially near the ostioles, 200-375
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<normalizedToken originalValue="×">x</normalizedToken>
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195-280
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; with 1-3(-6) papillate ostioles; pycnidial wall pseudoparenchymatous, 3-5 layers, 10-35
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<normalizedToken originalValue="μm">μm</normalizedToken>
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thick, outer layers composed of pale brown, flattened polygonal cells of 16-32
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<normalizedToken originalValue="μm">μm</normalizedToken>
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diam.
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<emphasis italics="true" pageId="0" pageNumber="49">Conidiogenous cells</emphasis>
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phialidic, hyaline, smooth, ampulliform, lageniform or somewhat isodiametric, (4.5-)5.5-10
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<normalizedToken originalValue="×">x</normalizedToken>
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3.5-9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="49">Conidia</emphasis>
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ellipsoidal to oblong, straight, thin- and smooth-walled, hyaline, aseptate, 3.5-7
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<normalizedToken originalValue="×">x</normalizedToken>
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2-3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, 2-guttulate, big.
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<taxonomicName genus="Conidial" lsidName="Conidial matrix" pageId="0" pageNumber="49" rank="species" species="matrix">
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<emphasis italics="true" pageId="0" pageNumber="49">Conidial matrix</emphasis>
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</taxonomicName>
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buff.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="49">Colonies after 7 d at 25 °C, on OA reaching 55-60 mm diam, aerial mycelium floccose, pale smoke grey, pale brown towards periphery, abundant production of confluent pycnidia, margin regular; reverse pale olivaceous, with some olivaceous black zones. On MEA reaching 50-55 mm diam, aerial mycelium woolly, pale olivaceous grey, margin irregular; reverse buff near the centre, dark brown with orange edge. On PDA reaching 50-55 mm diam, aerial mycelium floccose, pale mouse grey with olivaceous edge, margin irregular; reverse dark brown with pale brown edge. NaOH spot test negative on OA.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="notes">
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<paragraph pageId="0" pageNumber="49">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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Based on the multi-gene phylogenetic analyses,
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<taxonomicName lsidName="D. kooimaniorum" pageId="0" pageNumber="49" rank="species" species="kooimaniorum">
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<emphasis italics="true" pageId="0" pageNumber="49">D. kooimaniorum</emphasis>
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</taxonomicName>
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forms an independent branch, clearly separated from other species in
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<taxonomicName class="Dothideomycetes" family="Didymellaceae" genus="Didymella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Didymella" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="49">Didymella</emphasis>
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</taxonomicName>
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(Figure
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogenetic tree generated from the maximum-likelihood analysis based on the combined ITS, LSU, tub 2 and rpb 2 sequence alignment of Didymellaceae members. The RAxML bootstrap support values (BS), Bayesian posterior probabilities (PP), and parsimony bootstrap support values (PBS) are given at the nodes (BS / PP / PBS). BS and PBS values represent parsimony bootstrap support values> 50 %. Full supported branches are indicated in bold. The scale bar represents the expected number of changes per site. Ex-type strains are represented in bold. Strains obtained in the current study are printed in green; among them, whilst strains that represent new taxa are printed in red. Some of the basal branches were shortened to facilitate layout (the fraction in round parentheses refers to the presented length compared to the actual length of the branch). The tree was rooted to Coniothyrium palmarum CBS 400.71 and Leptosphaeria doliolum CBS 505.75." figureDoi="10.3897/mycokeys.65.47704.figure1" httpUri="https://binary.pensoft.net/fig/388204" pageId="0" pageNumber="49">1</figureCitation>
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). Morphologically,
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<taxonomicName lsidName="D. kooimaniorum" pageId="0" pageNumber="49" rank="species" species="kooimaniorum">
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<emphasis italics="true" pageId="0" pageNumber="49">D. kooimaniorum</emphasis>
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</taxonomicName>
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is characterised by pale brown pycnidia densely covered by long hairs, and ostioles with up to six papillae with a darker neck.
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</paragraph>
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<caption doi="10.3897/mycokeys.65.47704.figure4" httpUri="https://binary.pensoft.net/fig/388207" pageId="0" pageNumber="49" start="Figure 4" startId="F4">
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<paragraph pageId="0" pageNumber="49">
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<emphasis bold="true" pageId="0" pageNumber="49">Figure 4.</emphasis>
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<taxonomicName authorityName="Hern. - Restr., L. W. Hou, L. Cai & Crous" authorityYear="2020" class="Dothideomycetes" family="Didymellaceae" genus="Didymella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Didymella kooimaniorum" order="Pleosporales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="kooimaniorum">
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<emphasis italics="true" pageId="0" pageNumber="49">Didymella kooimaniorum</emphasis>
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</taxonomicName>
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(CBS 144951).
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<emphasis bold="true" pageId="0" pageNumber="49">A, B</emphasis>
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Colony on OA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">C, D</emphasis>
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colony on MEA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">E, F</emphasis>
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colony on PDA (front and reverse)
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<emphasis bold="true" pageId="0" pageNumber="49">G</emphasis>
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pycnidia forming on OA
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<emphasis bold="true" pageId="0" pageNumber="49">H</emphasis>
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pycnidia
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<emphasis bold="true" pageId="0" pageNumber="49">I</emphasis>
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section of pycnidium
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<emphasis bold="true" pageId="0" pageNumber="49">J</emphasis>
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section of pycnidial wall
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<emphasis bold="true" pageId="0" pageNumber="49">K-M</emphasis>
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conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="49">N</emphasis>
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conidia. Scale bars: 100
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">H</emphasis>
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); 50
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">I</emphasis>
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); 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">J</emphasis>
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); 5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">K-N</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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</treatment>
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</document> |