246 lines
18 KiB
XML
246 lines
18 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.78.60878" ID-GBIF-Dataset="07ad5422-fc25-45dd-be81-03cb609ac30e" ID-PMC="PMC7910272" ID-Pensoft-Pub="1314-4049-78-49" ID-Pensoft-UUID="5BCC2C78F2F856C3B93134F805790F02" ID-PubMed="33664613" ModsDocID="1314-4049-78-49" checkinTime="1613833639718" checkinUser="pensoft" docAuthor="Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Sun, Wenxiu" docDate="2021" docId="F02A5541B8BA55D292D9190AD164B410" docLanguage="en" docName="MycoKeys 78: 49-77" docOrigin="MycoKeys 78" docPubDate="2021-02-19" docSource="http://dx.doi.org/10.3897/mycokeys.78.60878" docTitle="Diaporthe chrysalidocarpi S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang 2021, sp. nov." docType="treatment" docVersion="4" id="5BCC2C78F2F856C3B93134F805790F02" lastPageNumber="49" masterDocId="5BCC2C78F2F856C3B93134F805790F02" masterDocTitle="Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China" masterLastPageNumber="77" masterPageNumber="49" pageNumber="49" updateTime="1668136623939" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-02-19</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>78</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>49</mods:start>
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<mods:end>77</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.78.60878</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.78.60878</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-78-49</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5BCC2C78F2F856C3B93134F805790F02</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="177488918" LSID="urn:lsid:plazi:treatment:F02A5541B8BA55D292D9190AD164B410" httpUri="http://treatment.plazi.org/id/F02A5541B8BA55D292D9190AD164B410" lastPageNumber="49" pageId="0" pageNumber="49">
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<subSubSection pageId="0" pageNumber="49" type="nomenclature">
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName LSID="F02A5541-B8BA-55D2-92D9-190AD164B410" authority="S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang" authorityName="S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe chrysalidocarpi" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="chrysalidocarpi" status="sp. nov.">Diaporthe chrysalidocarpi S.T. Huang, J.W. Xia, W.X. Sun, & X.G. Zhang</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="49">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 3" captionStartId="F5" captionText="Figure 3. Diaporthe chrysalidocarpi (SAUCC 194.35) a diseased leaf of Chrysalidocarpus lutescens b, c surface and reverse of a colony after 15 days on PDA d, e conidiomata f, g conidiophores and conidiogenous cells h, i beta conidia. Scale bars: 10 μm (f-i)." figureDoi="10.3897/mycokeys.78.60878.figure3" httpUri="https://binary.pensoft.net/fig/511678" pageId="0" pageNumber="49">Figure 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="etymology">
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<paragraph pageId="0" pageNumber="49">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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Named after the host genus on which it was collected,
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<taxonomicName class="Liliopsida" family="Palmae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="49">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="diagnosis">
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<paragraph pageId="0" pageNumber="49">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang" authorityYear="2021" class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe chrysalidocarpi" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe chrysalidocarpi</emphasis>
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</taxonomicName>
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can be distinguished from the phylogenetically most closely related species
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<taxonomicName lsidName="D. spinosa" pageId="0" pageNumber="49" rank="species" species="spinosa">
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<emphasis italics="true" pageId="0" pageNumber="49">D. spinosa</emphasis>
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</taxonomicName>
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by longer beta conidia (28.0-32.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-1.6 vs. 18.5-30.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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), and from other species
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<taxonomicName lsidName="D. fulvicolor" pageId="0" pageNumber="49" rank="species" species="fulvicolor">
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<emphasis italics="true" pageId="0" pageNumber="49">D. fulvicolor</emphasis>
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</taxonomicName>
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by the types of conidia (
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<taxonomicName lsidName="D. chrysalidocarpi" pageId="0" pageNumber="49" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">D. chrysalidocarpi</emphasis>
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</taxonomicName>
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produces only beta conidia, while
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<taxonomicName lsidName="D. fulvicolor" pageId="0" pageNumber="49" rank="species" species="fulvicolor">
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<emphasis italics="true" pageId="0" pageNumber="49">D. fulvicolor</emphasis>
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</taxonomicName>
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produces only alpha conidia) and several loci (25/491 in the ITS region, 18/471 TUB, 4/298 TEF, 28/458 CAL and 13/441 HIS).
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="materials_examined">
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<paragraph pageId="0" pageNumber="49">Type.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on diseased leaves of
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<taxonomicName class="Liliopsida" family="Palmae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="49">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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(
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<taxonomicName authorityName="H.Wendland" authorityYear="1878" class="Liliopsida" family="Palmae" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="family">Palmae</taxonomicName>
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). 19 April 2019, S.T. Huang, HSAUP194.35 holotype, ex-type living culture SAUCC194.35.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Description.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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Asexual morph: Leaf spots irregular, pale brown in center, brown to tan at margin. Conidiomata pycnidial, scattered or aggregated, black, erumpent, raising above surface of culture medium, subglobose, exuding white or yellowish creamy conidial droplets from central ostioles after 30 days in light at 25 °C; pycnidial wall consists of black to dark brown, thin-walled cells. Conidiophores 27.5-35.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4-2.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, hyaline, slightly branched, swelling at base, subcylindrical, septate, smooth, straight or curved. Conidiogenous cells 10.5-23.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4-1.8
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, phialidic, cylindrical, terminal, straight to sinuous, tapering towards apex. Beta conidia 28.0-32.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-1.6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(mean = 30.3
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<normalizedToken originalValue="×">x</normalizedToken>
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1.3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20), filiform, hyaline, straight or slightly curved, aseptate, base subtruncate, tapering towards the base. Alpha conidia and gamma conidia not observed. Sexual morph not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="49">Cultures incubated on PDA at 25 °C in darkness, growth rate 13.3-15.2 mm diam/day, initially white, becoming greyish, reverse pale brown, with concentric rings of dense, sparse hyphae, irregular margin, fluffy aerial mycelium at center, pycnidia forming after 15 days.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="additional specimen examined">
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<paragraph pageId="0" pageNumber="49">Additional specimen examined.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on diseased leaves of
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<taxonomicName class="Liliopsida" family="Palmae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="49">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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(
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<taxonomicName authorityName="H.Wendland" authorityYear="1878" class="Liliopsida" family="Palmae" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="family">Palmae</taxonomicName>
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). 19 April 2019, S.T. Huang, HSAUP194.33 paratype; living culture SAUCC194.33.
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</paragraph>
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<caption doi="10.3897/mycokeys.78.60878.figure3" httpUri="https://binary.pensoft.net/fig/511678" pageId="0" pageNumber="49" start="Figure 3" startId="F5">
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<paragraph pageId="0" pageNumber="49">
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<emphasis bold="true" pageId="0" pageNumber="49">Figure 3.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang" authorityYear="2021" class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe chrysalidocarpi" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe chrysalidocarpi</emphasis>
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</taxonomicName>
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(SAUCC194.35)
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<emphasis bold="true" pageId="0" pageNumber="49">a</emphasis>
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diseased leaf of
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<taxonomicName class="Liliopsida" family="Palmae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="49" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="49">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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<emphasis bold="true" pageId="0" pageNumber="49">b, c</emphasis>
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surface and reverse of a colony after 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="49">d, e</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="49">f, g</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="49">h, i</emphasis>
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beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">f-i</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="notes">
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<paragraph pageId="0" pageNumber="49">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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Phylogenetic analysis of a combined five gene showed that
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<taxonomicName lsidName="D. chrysalidocarpi" pageId="0" pageNumber="49" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">D. chrysalidocarpi</emphasis>
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</taxonomicName>
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formed an independent clade (Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe spp. based on combined sequence data of ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Codes referring to strains from the current study are written in red. Some branches were shortened to fit them to the page as indicated by two diagonal lines with the number of times a branch was shortened indicated." figureDoi="10.3897/mycokeys.78.60878.figure1" httpUri="https://binary.pensoft.net/fig/511674" pageId="0" pageNumber="49">1</figureCitation>
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) and is phylogenetically distinct from
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<taxonomicName lsidName="D. spinosa" pageId="0" pageNumber="49" rank="species" species="spinosa">
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<emphasis italics="true" pageId="0" pageNumber="49">D. spinosa</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. fulvicolor" pageId="0" pageNumber="49" rank="species" species="fulvicolor">
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<emphasis italics="true" pageId="0" pageNumber="49">D. fulvicolor</emphasis>
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</taxonomicName>
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. This species can be distinguished from
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<taxonomicName lsidName="D. spinosa" pageId="0" pageNumber="49" rank="species" species="spinosa">
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<emphasis italics="true" pageId="0" pageNumber="49">D. spinosa</emphasis>
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</taxonomicName>
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by 61 different nucleotides in the concatenated alignment (13/492 in the ITS region, 17/471 TUB, 4/298 TEF, 17/458 CAL and 10/441 HIS), and
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<taxonomicName lsidName="D. fulvicolor" pageId="0" pageNumber="49" rank="species" species="fulvicolor">
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<emphasis italics="true" pageId="0" pageNumber="49">D. fulvicolor</emphasis>
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</taxonomicName>
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by 88 nucleotides (25/491 in the ITS region, 18/471 TUB, 4/298 TEF, 28/458 CAL and 13/441 HIS). Morphologically,
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<taxonomicName lsidName="D. chrysalidocarpi" pageId="0" pageNumber="49" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">D. chrysalidocarpi</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. spinosa" pageId="0" pageNumber="49" rank="species" species="spinosa">
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<emphasis italics="true" pageId="0" pageNumber="49">D. spinosa</emphasis>
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</taxonomicName>
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in having longer beta conidia (28.0-32.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-1.6 vs. 18.5-30.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) (
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<bibRefCitation DOI="https://doi.org/10.3767/persoonia.2020.45.05" author="Guo, YS" journalOrPublisher="Persoonia" pageId="0" pageNumber="49" pagination="132 - 162" refId="B22" refString="Guo, YS, Crous, PW, Bai, Q, Fu, M, Yang, MM, Wang, XH, Du, YM, Hong, N, Xu, WX, Wang, GP, 2020. High diversity of Diaporthe species associated with pear shoot canker in China. Persoonia 45: 132 - 162, DOI: https://doi.org/10.3767/persoonia.2020.45.05" title="High diversity of Diaporthe species associated with pear shoot canker in China." url="https://doi.org/10.3767/persoonia.2020.45.05" volume="45" year="2020">Guo et al. 2020</bibRefCitation>
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). Furthermore,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, W. X. Sun, & X. G. Zhang" authorityYear="2021" class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe chrysalidocarpi" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="chrysalidocarpi">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe chrysalidocarpi</emphasis>
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</taxonomicName>
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produces only beta conidia, while
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<taxonomicName lsidName="D. spinosa" pageId="0" pageNumber="49" rank="species" species="spinosa">
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<emphasis italics="true" pageId="0" pageNumber="49">D. spinosa</emphasis>
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</taxonomicName>
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produces alpha conidia and beta conidia and
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<taxonomicName lsidName="D. fulvicolor" pageId="0" pageNumber="49" rank="species" species="fulvicolor">
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<emphasis italics="true" pageId="0" pageNumber="49">D. fulvicolor</emphasis>
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</taxonomicName>
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produces only alpha conidia (
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<bibRefCitation DOI="https://doi.org/10.3767/persoonia.2020.45.05" author="Guo, YS" journalOrPublisher="Persoonia" pageId="0" pageNumber="49" pagination="132 - 162" refId="B22" refString="Guo, YS, Crous, PW, Bai, Q, Fu, M, Yang, MM, Wang, XH, Du, YM, Hong, N, Xu, WX, Wang, GP, 2020. High diversity of Diaporthe species associated with pear shoot canker in China. Persoonia 45: 132 - 162, DOI: https://doi.org/10.3767/persoonia.2020.45.05" title="High diversity of Diaporthe species associated with pear shoot canker in China." url="https://doi.org/10.3767/persoonia.2020.45.05" volume="45" year="2020">Guo et al. 2020</bibRefCitation>
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). Therefore, we establish this fungus as a novel species.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |