276 lines
47 KiB
XML
276 lines
47 KiB
XML
<document id="4540927CECF4BE402F17226C7D41D9F9" ID-DOI="10.14411/fp.2020.033" ID-ISSN="1803-6465" ID-Zenodo-Dep="8143626" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="julia" IM.tables_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1689253332823" checkinUser="felipe" docAuthor="Krupenko, Darya, Gonchar, Anna, Kremnev, Georgii, Efeykin, Boris, Krapivin, Vladimir & Methods, Materials And" docDate="2020" docId="03F187EEFF9CFFBE9F7D869D3BEAB30C" docLanguage="en" docName="FoliaParasit.67.033.pdf" docOrigin="Folia Parasitologica (033) 67" docSource="http://dx.doi.org/10.14411/fp.2020.033" docStyle="DocumentStyle:7565043637801AC5878175AE945D3331.1:FoliaParasit.2015-.journal_article" docStyleId="7565043637801AC5878175AE945D3331" docStyleName="FoliaParasit.2015-.journal_article" docStyleVersion="1" docTitle="Cercaria cattieni" docType="treatment" docVersion="1" lastPageNumber="3" masterDocId="FFC8FF96FF9DFFBD9C50801D3850B27B" masterDocTitle="New type of xiphidiocercariae (Digenea: Microphalloidea) from South Vietnam" masterLastPageNumber="6" masterPageNumber="1" pageNumber="2" updateTime="1690218379658" updateUser="julia" zenodo-license-document="CC-BY-4.0">
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<mods:titleInfo id="62EB1E3F4FDCE8C2FE9DF6BED3282B14">
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<mods:title id="883AF73C9E7DC6663E764CC37634ACA7">New type of xiphidiocercariae (Digenea: Microphalloidea) from South Vietnam</mods:title>
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<mods:name id="A90187108AEF0FC1A35429CA722BE47C" type="personal">
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<mods:roleTerm id="89F1B267FD763298E0AF9220B079730B">Author</mods:roleTerm>
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<mods:namePart id="334D202E1656A3FDEE0140C5A1E2D862">Krupenko, Darya</mods:namePart>
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<mods:affiliation id="7815A4D999EA170CC81F3439965097C5">Saint Petersburg University, Department of Invertebrate Zoology, Saint Petersburg, Russia;</mods:affiliation>
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<mods:roleTerm id="C7236185B9D6294FC27759B66F336414">Author</mods:roleTerm>
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<mods:namePart id="605C70BD7CD1F4CFE820627060476157">Gonchar, Anna</mods:namePart>
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<mods:affiliation id="744C974B8177A1F4C42FE57F0D26AB3B">Saint Petersburg University, Department of Invertebrate Zoology, Saint Petersburg, Russia; & Zoological Institute, Laboratory of Parasitic Worms and Protists, Russian Academy of Sciences, Saint Petersburg, Russia;</mods:affiliation>
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<mods:roleTerm id="A9C260C3C4D276872FA5252FBA6F4673">Author</mods:roleTerm>
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<mods:namePart id="F9AFD892C42BCBEB6E5D6B5376B87688">Kremnev, Georgii</mods:namePart>
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<mods:affiliation id="952DC820770C80E9BAFD9B3E9F0F2940">Saint Petersburg University, Department of Invertebrate Zoology, Saint Petersburg, Russia;</mods:affiliation>
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</mods:name>
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<mods:roleTerm id="08919AAC5A297342FDFC7FBF6FD347A7">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="D23366ED2E6E632104A677EC93F18795">Efeykin, Boris</mods:namePart>
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<mods:affiliation id="2604A17DE6CE3C2F6DC26A670A0DE65C">Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia; & The Russian-Vietnamese Tropical Scientific and Technological Centre, Southern Branch, Ho Chi Minh, Vietnam; & Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia</mods:affiliation>
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</mods:name>
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<mods:name id="F15E1ABF41E2BE64B9DB4BEC5F513056" type="personal">
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<mods:roleTerm id="4C76D0119186080411D09B83A222E1F1">Author</mods:roleTerm>
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<mods:namePart id="559262AD63480D4E4275BFFF7096BA8A">Krapivin, Vladimir</mods:namePart>
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<mods:affiliation id="5E669D964CC9F6F96BB439DCC89763E9">Saint Petersburg University, Department of Invertebrate Zoology, Saint Petersburg, Russia; & Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia; & The Russian-Vietnamese Tropical Scientific and Technological Centre, Southern Branch, Ho Chi Minh, Vietnam;</mods:affiliation>
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<mods:namePart id="CC0AB598306C1348AE72FFBBD259B5BC">Methods, Materials And</mods:namePart>
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<mods:typeOfResource id="B0A6F9AB7CC072550A11CF4314F75CB5">text</mods:typeOfResource>
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<mods:title id="A6ABA36C8953C8F1662D45492A623EC6">Folia Parasitologica</mods:title>
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<mods:part id="82C6AA6A6DCE2BDCDAF0E1DDE3EB60C0">
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<mods:date id="05C62A3080686BEBFBAC8067128813A5">2020</mods:date>
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<mods:title id="3A3496A77A208B0CF9DC90D86BDD9F27">033</mods:title>
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<mods:number id="0A2C10641AE8811A68D0AF20D7E1297A">2020-11-27</mods:number>
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<mods:number id="C88374EC01068B1D265E1E8F1272DC35">67</mods:number>
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<mods:start id="CC325B0A29088DB88AF074FE998E5D6A">1</mods:start>
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<mods:url id="0E1E0AFF46292D6F8A51D599F49F5483">http://dx.doi.org/10.14411/fp.2020.033</mods:url>
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<mods:classification id="7918D5EA508FE1DBD2EF9D35F8B50F34">journal article</mods:classification>
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<mods:identifier id="D79279CA1EF2CF5D4A9D3DA34CA64AC6" type="DOI">10.14411/fp.2020.033</mods:identifier>
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<mods:identifier id="1C27D13577B4EFDF782B88C4259EC745" type="ISSN">1803-6465</mods:identifier>
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<mods:identifier id="3A83CC3ABCDB69314399037CB25DB7F3" type="Zenodo-Dep">8143626</mods:identifier>
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<treatment id="03F187EEFF9CFFBE9F7D869D3BEAB30C" LSID="urn:lsid:plazi:treatment:03F187EEFF9CFFBE9F7D869D3BEAB30C" httpUri="http://treatment.plazi.org/id/03F187EEFF9CFFBE9F7D869D3BEAB30C" lastPageId="3" lastPageNumber="3" pageId="1" pageNumber="2">
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<subSubSection id="C3426573FF9CFFBC9F7D869D3DC1B4EC" box="[813,1425,1663,1687]" pageId="1" pageNumber="2" type="nomenclature">
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<paragraph id="8BE736F8FF9CFFBC9F7D869D3DC1B4EC" blockId="1.[813,1425,1663,1687]" box="[813,1425,1663,1687]" pageId="1" pageNumber="2">
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<taxonomicName id="4C584D7BFF9CFFBC9F7D869D3BB3B4EC" box="[813,995,1664,1687]" class="Trematoda" family="Aporocotylidae" genus="Cercaria" kingdom="Animalia" order="Diplostomida" pageId="1" pageNumber="2" phylum="Platyhelminthes" rank="species" species="cattieni">
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<emphasis id="B92CEAEAFF9CFFBC9F7D869D3BB3B4EC" box="[813,995,1664,1687]" italics="true" pageId="1" pageNumber="2">Cercaria cattieni</emphasis>
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</taxonomicName>
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1
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<figureCitation id="13632A7DFF9CFFBC990386623DC1B4EC" box="[1363,1425,1663,1687]" captionStart="Fig" captionStartId="1.[151,186,1918,1940]" captionTargetBox="[166,740,475,1857]" captionTargetId="figure-566@1.[173,753,462,1887]" captionTargetPageId="1" captionText="Fig. 1. Cercaria cattieni 1. A – sporocyst; B–F – cercariae; B – body structure; C – general view; D, E – stylet, ventral (D) and lateral (E) view;F – scheme of cercarial embryo with mucoid glands (grey)." figureDoi="http://doi.org/10.5281/zenodo.8143628" httpUri="https://zenodo.org/record/8143628/files/figure.png" pageId="1" pageNumber="2">Fig. 1</figureCitation>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="C3426573FF9CFFBC9F7D86B33D6AB49A" pageId="1" pageNumber="2" type="biology_ecology">
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<paragraph id="8BE736F8FF9CFFBC9F7D86B33D6AB49A" blockId="1.[813,1438,1709,2073]" pageId="1" pageNumber="2">
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F i r s t i n t e r m e d i a t e h o s t:
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<taxonomicName id="4C584D7BFF9CFFBC98DC86B03BB3B49A" authority="(Morelet, 1884)" authorityName="Caenogastropoda" baseAuthorityName="Morelet" baseAuthorityYear="1885" class="Gastropoda" family="Pachychilidae" genus="Sulcospira" kingdom="Animalia" pageId="1" pageNumber="2" phylum="Mollusca" rank="species" species="dautzenbergiana">
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<emphasis id="B92CEAEAFF9CFFBC98DC86B03DCCB4B9" box="[1164,1436,1709,1730]" italics="true" pageId="1" pageNumber="2">Sulcospira dautzenbergiana</emphasis>
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(Morelet, 1884)
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</taxonomicName>
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(Caenogastropoda:
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<taxonomicName id="4C584D7BFF9CFFBC98F486D63D7FB49A" authorityName="P. Fischer & Crosse" authorityYear="1892" box="[1188,1327,1739,1761]" class="Gastropoda" family="Pachychilidae" kingdom="Animalia" pageId="1" pageNumber="2" phylum="Mollusca" rank="family">Pachychilidae</taxonomicName>
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).
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</paragraph>
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</subSubSection>
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<subSubSection id="C3426573FF9CFFBC9F7D86F13C99B51A" pageId="1" pageNumber="2" type="description">
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<paragraph id="8BE736F8FF9CFFBC9F7D86F13C39B564" blockId="1.[813,1438,1709,2073]" pageId="1" pageNumber="2">
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<materialsCitation id="3B303CA5FF9CFFBC9F7D86F13C35B564" country="Vietnam" latitude="11.4305" location="Stream in Cat Tien National Park" longLatPrecision="7" longitude="107.4294" municipality="Southern" pageId="1" pageNumber="2" specimenCount="1">
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L o c a l i t y:
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<location id="8E876023FF9CFFBC9FE686F13D52B579" LSID="urn:lsid:plazi:treatment:03F187EEFF9CFFBE9F7D869D3BEAB30C:8E876023FF9CFFBC9FE686F13D52B579" box="[950,1282,1772,1794]" country="Vietnam" latitude="11.4305" longLatPrecision="7" longitude="107.4294" municipality="Southern" name="Stream in Cat Tien National Park" pageId="1" pageNumber="2">Stream in Cát Tiên National Park</location>
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,
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<collectingMunicipality id="6B83AC82FF9CFFBC995E86F13D35B579" box="[1294,1381,1772,1794]" pageId="1" pageNumber="2">Southern</collectingMunicipality>
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<collectingCountry id="F34F7668FF9CFFBC993C86F13B24B564" name="Vietnam" pageId="1" pageNumber="2">Vietnam</collectingCountry>
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(
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<geoCoordinate id="EE6C503FFF9CFFBC9FD287143BB3B564" box="[898,995,1801,1823]" degrees="11.4305" direction="north" orientation="latitude" pageId="1" pageNumber="2" precision="5" value="11.4305">11.4305N</geoCoordinate>
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,
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<geoCoordinate id="EE6C503FFF9CFFBC9FBD87143C06B565" box="[1005,1110,1801,1822]" degrees="107.4294" direction="east" orientation="longitude" pageId="1" pageNumber="2" precision="5" value="107.4294">107.4294E</geoCoordinate>
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)
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</materialsCitation>
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.
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</paragraph>
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<paragraph id="8BE736F8FF9CFFBC9F7D87363C01B53A" blockId="1.[813,1438,1709,2073]" box="[813,1105,1834,1857]" pageId="1" pageNumber="2">P r e v a l e n c e: 2% (n = 41).</paragraph>
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<paragraph id="8BE736F8FF9CFFBC9F7D87513C99B51A" blockId="1.[813,1438,1709,2073]" box="[813,1225,1867,1889]" pageId="1" pageNumber="2">S i t e o f i n f e c t i o n: Digestive gland.</paragraph>
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</subSubSection>
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<subSubSection id="C3426573FF9CFFBC9F7D87703CF5B5A1" pageId="1" pageNumber="2" type="materials_examined">
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<paragraph id="8BE736F8FF9CFFBC9F7D87703CF5B5A1" blockId="1.[813,1438,1709,2073]" pageId="1" pageNumber="2">
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Vo u c h e r s p e c i m e n s: Five slides from one gastropod specimen were placed in the collection of the Department of Invertebrate Zoology of
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<collectingRegion id="499CF81AFF9CFFBC981887BA3CBBB5C6" box="[1096,1259,1959,1981]" country="Russia" name="Saint Petersburg" pageId="1" pageNumber="2">Saint Petersburg</collectingRegion>
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University under numbers
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<date id="FFE61038FF9CFFBC9FF387D93BB8B5A1" box="[931,1000,1988,2010]" pageId="1" pageNumber="2" value="2018-05">V2018</date>
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F
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<date id="FFE61038FF9CFFBC9FA687D93C3EB5A1" box="[1014,1134,1988,2010]" pageId="1" pageNumber="2" value="2018-05-01" valueMax="2018-05-01" valueMin="2018-05-01">01-1–V2018</date>
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F01-5
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</paragraph>
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</subSubSection>
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||
<caption id="DF276670FF9CFFBC9CC78763394DB593" ID-DOI="http://doi.org/10.5281/zenodo.8143628" ID-Zenodo-Dep="8143628" httpUri="https://zenodo.org/record/8143628/files/figure.png" pageId="1" pageNumber="2" startId="1.[151,186,1918,1940]" targetBox="[166,740,475,1857]" targetPageId="1" targetType="figure">
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<paragraph id="8BE736F8FF9CFFBC9CC78763394DB593" blockId="1.[150,775,1918,2024]" pageId="1" pageNumber="2">
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<emphasis id="B92CEAEAFF9CFFBC9CC78763388CB5E8" bold="true" box="[151,220,1918,1940]" pageId="1" pageNumber="2">Fig. 1.</emphasis>
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<taxonomicName id="4C584D7BFF9CFFBC9CB7876339C1B5EF" box="[231,401,1918,1940]" class="Trematoda" family="Aporocotylidae" genus="Cercaria" kingdom="Animalia" order="Diplostomida" pageId="1" pageNumber="2" phylum="Platyhelminthes" rank="species" species="cattieni">
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<emphasis id="B92CEAEAFF9CFFBC9CB7876339C1B5EF" box="[231,401,1918,1940]" italics="true" pageId="1" pageNumber="2">Cercaria cattieni</emphasis>
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</taxonomicName>
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1.
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<emphasis id="B92CEAEAFF9CFFBC9DEA8763399BB5E8" bold="true" box="[442,459,1918,1939]" pageId="1" pageNumber="2">A</emphasis>
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– sporocyst;
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<emphasis id="B92CEAEAFF9CFFBC9E0C87633AD7B5E8" bold="true" box="[604,647,1918,1939]" pageId="1" pageNumber="2">B–F</emphasis>
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– cercariae;
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<emphasis id="B92CEAEAFF9CFFBC9CC7878738F7B5D4" bold="true" box="[151,167,1946,1967]" pageId="1" pageNumber="2">B</emphasis>
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– body structure;
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<emphasis id="B92CEAEAFF9CFFBC9D0C8787393DB5CB" bold="true" box="[348,365,1946,1968]" pageId="1" pageNumber="2">C</emphasis>
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– general view;
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<emphasis id="B92CEAEAFF9CFFBC9E4287873A72B5D4" bold="true" box="[530,546,1946,1967]" pageId="1" pageNumber="2">D</emphasis>
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,
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<emphasis id="B92CEAEAFF9CFFBC9E6087873A10B5D4" bold="true" box="[560,576,1946,1967]" pageId="1" pageNumber="2">E</emphasis>
|
||
– stylet, ventral (D) and lateral (E) view;
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<emphasis id="B92CEAEAFF9CFFBC9D0E87AB393DB5B0" bold="true" box="[350,365,1974,1995]" pageId="1" pageNumber="2">F</emphasis>
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– scheme of cercarial embryo with mucoid glands (grey).
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</paragraph>
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</caption>
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<subSubSection id="C3426573FF9CFFBE9F7D87FB3BEAB30C" lastPageId="3" lastPageNumber="4" pageId="1" pageNumber="2" type="description">
|
||
<paragraph id="8BE736F8FF9CFFBF9F7D87FB3A85B749" blockId="1.[813,1438,1709,2073]" lastBlockId="2.[151,777,1278,1774]" lastPageId="2" lastPageNumber="3" pageId="1" pageNumber="2">
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Daughter sporocysts (measurements based on six fixed specimens;
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<figureCitation id="13632A7DFF9CFFBC989488193D10BA63" box="[1220,1344,2051,2073]" captionStart="Fig" captionStartId="1.[151,186,1918,1940]" captionTargetBox="[166,740,475,1857]" captionTargetId="figure-566@1.[173,753,462,1887]" captionTargetPageId="1" captionText="Fig. 1. Cercaria cattieni 1. A – sporocyst; B–F – cercariae; B – body structure; C – general view; D, E – stylet, ventral (D) and lateral (E) view;F – scheme of cercarial embryo with mucoid glands (grey)." figureDoi="http://doi.org/10.5281/zenodo.8143628" httpUri="https://zenodo.org/record/8143628/files/figure.png" pageId="1" pageNumber="2">Figs. 1 A</figureCitation>
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,
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<figureCitation id="13632A7DFF9CFFBC9932881E3DD4BA63" box="[1378,1412,2051,2072]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="1" pageNumber="2">2 A</figureCitation>
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): Sporocysts white, elongate, 799–1,628 × 291–369 (1,124 × 336), contain cercariae at different stages of development.
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</paragraph>
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<caption id="DF276670FF9FFFBF9CC783E93920B6B5" ID-DOI="http://doi.org/10.5281/zenodo.8143630" ID-Zenodo-Dep="8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3" startId="2.[151,186,1012,1034]" targetBox="[165,1422,189,982]" targetPageId="2" targetType="figure">
|
||
<paragraph id="8BE736F8FF9FFFBF9CC783E93920B6B5" blockId="2.[151,1437,1012,1230]" pageId="2" pageNumber="3">
|
||
<emphasis id="B92CEAEAFF9FFFBF9CC783E93888B672" bold="true" box="[151,216,1012,1034]" pageId="2" pageNumber="3">Fig. 2.</emphasis>
|
||
Microphotographs of
|
||
<taxonomicName id="4C584D7BFF9FFFBF9DE683E83A0BB671" box="[438,603,1013,1034]" class="Trematoda" family="Aporocotylidae" genus="Cercaria" kingdom="Animalia" order="Diplostomida" pageId="2" pageNumber="3" phylum="Platyhelminthes" rank="species" species="cattieni">
|
||
<emphasis id="B92CEAEAFF9FFFBF9DE683E83A0BB671" box="[438,603,1013,1034]" italics="true" pageId="2" pageNumber="3">Cercaria cattieni</emphasis>
|
||
</taxonomicName>
|
||
1 stained with Erlich’s haematoxylin (
|
||
<emphasis id="B92CEAEAFF9FFFBF9F8783E93BB8B672" bold="true" box="[983,1000,1012,1033]" pageId="2" pageNumber="3">A</emphasis>
|
||
) and Heidenhain’s iron haematoxylin (
|
||
<emphasis id="B92CEAEAFF9FFFBF993983E93DDEB672" bold="true" box="[1385,1422,1012,1034]" pageId="2" pageNumber="3">B–I</emphasis>
|
||
).
|
||
<emphasis id="B92CEAEAFF9FFFBF9CC7840D38F8B65E" bold="true" box="[151,168,1040,1061]" pageId="2" pageNumber="3">A</emphasis>
|
||
– daughter sporocyst;
|
||
<emphasis id="B92CEAEAFF9FFFBF9DD2840D39C2B65E" bold="true" box="[386,402,1040,1061]" pageId="2" pageNumber="3">B</emphasis>
|
||
– underdeveloped cercaria;
|
||
<emphasis id="B92CEAEAFF9FFFBF9EF0840D3AE1B65D" bold="true" box="[672,689,1040,1062]" pageId="2" pageNumber="3">C</emphasis>
|
||
– cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible;
|
||
<emphasis id="B92CEAEAFF9FFFBF981E84313C0FB63A" bold="true" box="[1102,1119,1068,1089]" pageId="2" pageNumber="3">D</emphasis>
|
||
,
|
||
<emphasis id="B92CEAEAFF9FFFBF983B84313C2BB63A" bold="true" box="[1131,1147,1068,1089]" pageId="2" pageNumber="3">E</emphasis>
|
||
– spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on
|
||
<emphasis id="B92CEAEAFF9FFFBF9F4C84553B7CB626" bold="true" box="[796,812,1096,1117]" pageId="2" pageNumber="3">E</emphasis>
|
||
;
|
||
<emphasis id="B92CEAEAFF9FFFBF9F6984553B18B626" bold="true" box="[825,840,1096,1117]" pageId="2" pageNumber="3">F</emphasis>
|
||
– forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible;
|
||
<emphasis id="B92CEAEAFF9FFFBF9F6C84793B1EB601" bold="true" box="[828,846,1124,1146]" pageId="2" pageNumber="3">G</emphasis>
|
||
,
|
||
<emphasis id="B92CEAEAFF9FFFBF9F0B84793B3EB602" bold="true" box="[859,878,1124,1145]" pageId="2" pageNumber="3">H</emphasis>
|
||
– ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC);
|
||
<emphasis id="B92CEAEAFF9FFFBF9E52849D3A5BB6EE" bold="true" box="[514,523,1152,1173]" pageId="2" pageNumber="3">I</emphasis>
|
||
– hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BE736F8FF9FFFBF9CC785203AAAB495" blockId="2.[151,777,1278,1774]" pageId="2" pageNumber="3">
|
||
Cercariae (measurements based on 21 fixed s p e c i m e n s; F i g s. 1 B – E, 2 B – I): Body 481–758 (594) long, 285–460 (369) wide, pear-shaped, narrow at fore end. Hindbody wide, with folded margins. Tegument with simple spines, most densely arranged in forebody; largest spines in midbody. Tail simple, 603–841 (697) long, 69–101 (93) wide at base, with smooth tegument. Oral sucker subterminal, spherical, 69–103 × 66–102 (86 × 85). Its rim armed with simple spines thicker than body spines, their size reduces posteriorly (
|
||
<figureCitation id="13632A7DFF9FFFBF9D7E8658392FB420" box="[302,383,1605,1627]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2D</figureCitation>
|
||
). Stylet 43–52 (48) long, 5–8 (7) wide at base, single-pointed, with anterior thickening (
|
||
<figureCitation id="13632A7DFF9FFFBF9EC7867F3B52B40C" box="[663,770,1634,1656]" captionStart="Fig" captionStartId="1.[151,186,1918,1940]" captionTargetBox="[166,740,475,1857]" captionTargetId="figure-566@1.[173,753,462,1887]" captionTargetPageId="1" captionText="Fig. 1. Cercaria cattieni 1. A – sporocyst; B–F – cercariae; B – body structure; C – general view; D, E – stylet, ventral (D) and lateral (E) view;F – scheme of cercarial embryo with mucoid glands (grey)." figureDoi="http://doi.org/10.5281/zenodo.8143628" httpUri="https://zenodo.org/record/8143628/files/figure.png" pageId="2" pageNumber="3">Figs. 1D,E</figureCitation>
|
||
,
|
||
<figureCitation id="13632A7DFF9FFFBF9CE7869D3888B4ED" box="[183,216,1664,1686]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">2G</figureCitation>
|
||
). Ventral sucker spherical, 142–196 × 140–204 (160 × 165); 4–5 irregular rows of spines (thicker than body spines) lie at sucker rim, around opening, surrounded by smooth zone with 6 large papillae (probably, sensory receptors) (
|
||
<figureCitation id="13632A7DFF9FFFBF9EF486C53ABEB495" box="[676,750,1752,1774]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BE736F8FF9FFFBF9CE587023D29B72C" blockId="2.[150,775,1823,2071]" lastBlockId="2.[812,1438,1279,2071]" pageId="2" pageNumber="3">
|
||
Prepharynx short; pharynx 28–47 × 20–41 (37 × 28); oesophagus very short. Caecal primordia composed of single row of cells (
|
||
<figureCitation id="13632A7DFF9FFFBF9D01874239F4B50C" box="[337,420,1887,1911]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2F</figureCitation>
|
||
), 3–6 (4) wide, extending nearly to level of excretory vesicle. Paired cephalic ganglia conspicuous, posterior to oral sucker, interconnected by dorsal commissure between pharynx and oral sucker. Dorsal and ventral pairs of longitudinal nerve chords visible along whole body. Penetration glands numerous, in forebody, anterolateral to ventral sucker (
|
||
<figureCitation id="13632A7DFF9FFFBF982C84E23C9EB76C" box="[1148,1230,1279,1303]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2F</figureCitation>
|
||
). Ducts of penetration glands proceed along dorsal surface of oral sucker and open as two groups, lateral to stylet pouch (
|
||
<figureCitation id="13632A7DFF9FFFBF98AD85223D3DB72C" box="[1277,1389,1343,1367]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2G,H</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BE736F8FF9FFFBF9F1B857D3DC2B40C" blockId="2.[812,1438,1279,2071]" pageId="2" pageNumber="3">
|
||
Excretory vesicle I-shaped, 164–232 × 34–67 (189 × 47). Main collecting ducts starting from excretory vesicle anterolaterally (
|
||
<figureCitation id="13632A7DFF9FFFBF985585823C06B7CC" box="[1029,1110,1439,1463]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2I</figureCitation>
|
||
). Flame-cell formula not determined. Primordia of testes symmetrical, 12–15 (13) in diameter, anterolateral to excretory vesicle. Cirrus sac primordium anterior to ventral sucker. Primordia of uterus, Laurer’s channel and ovary also visible (
|
||
<figureCitation id="13632A7DFF9FFFBF988986023D74B44C" box="[1241,1316,1567,1591]" captionStart="Fig" captionStartId="2.[151,186,1012,1034]" captionTargetBox="[165,1422,189,982]" captionTargetId="figure-689@2.[165,1422,189,982]" captionTargetPageId="2" captionText="Fig. 2. Microphotographs of Cercaria cattieni 1 stained with Erlich’s haematoxylin (A) and Heidenhain’s iron haematoxylin (B–I). A – daughter sporocyst; B – underdeveloped cercaria; C – cercaria at late stage of development (tail lost during fixation); oral (os) and ventral (vs) sucker, excretory bladder (ex), primordia of cirrus sac (cs) and uterus (ut) are visible; D, E – spination of the oral sucker (D) and ventral sucker (E) rim (DIC); note sensory papillae (se) on E; F – forebody of cercaria (DIC), caeca (c), cephalic ganglia (cg), oral sucker (os), penetration glands (pg) and pharynx (ph) are visible; G, H – ducts of penetration glands going along the oral sucker, opening near the stylet (st) tip (DIC); I – hindbody of the cercaria (DIC); primordia of ovary (ov) and Laurer’s channel (lc) are visible posterior to the ventral sucker (vs); main collecting duct (mcd) starts anterolaterally from the excretory bladder (ex) and goes around testis primordium (te)." figureDoi="http://doi.org/10.5281/zenodo.8143630" httpUri="https://zenodo.org/record/8143630/files/figure.png" pageId="2" pageNumber="3">Fig. 2I</figureCitation>
|
||
). Cercariae swim with their body contracted, thus taking saucer-shape; elongated tail beats vigorously (Supplementary material).
|
||
</paragraph>
|
||
<paragraph id="8BE736F8FF9FFFBF9F1B86623D01B5EC" blockId="2.[812,1438,1279,2071]" pageId="2" pageNumber="3">
|
||
Mucoid glands found in cercarial embryos as four pairs of cells, anterior, lateral and posterior to ventral sucker (
|
||
<figureCitation id="13632A7DFF9FFFBF9F6586A23BD5B4AC" box="[821,901,1727,1751]" captionStart="Fig" captionStartId="1.[151,186,1918,1940]" captionTargetBox="[166,740,475,1857]" captionTargetId="figure-566@1.[173,753,462,1887]" captionTargetPageId="1" captionText="Fig. 1. Cercaria cattieni 1. A – sporocyst; B–F – cercariae; B – body structure; C – general view; D, E – stylet, ventral (D) and lateral (E) view;F – scheme of cercarial embryo with mucoid glands (grey)." figureDoi="http://doi.org/10.5281/zenodo.8143628" httpUri="https://zenodo.org/record/8143628/files/figure.png" pageId="2" pageNumber="3">Fig. 1F</figureCitation>
|
||
,
|
||
<figureCitation id="13632A7DFF9FFFBF9FC486A23BE8B4AC" box="[916,952,1727,1751]" captionStart="Fig" captionStartId="3.[151,186,1505,1527]" captionTargetBox="[151,775,189,1482]" captionTargetId="figure-685@3.[151,775,188,1482]" captionTargetPageId="3" captionText="Fig. 3. Toluidine blue staining of Cercaria cattieni 1. A – cercarial embryo, lateral view, early stage of mucoid glands (mg) formation; B – cercarial embryos at different stage, metachromatic staining shows mucoid glands; C – later cercarial embryo (tail lost during fixation), metachromatic staining of mucoid glands with numerous dendrites; D – mucoid staining of stylet pouch; E – mucoid on the surface of the fore region; F, G – mucoid in the midbody, beneath musculature and tegument; ventral nerve chords (vnc) are visible among mucoid; H – midbody, mucoid transferred to the tegument. os – oral sucker; vs – ventral sucker; t – tail. A, D, E, G, H – differential interference contrast." figureDoi="http://doi.org/10.5281/zenodo.8143632" httpUri="https://zenodo.org/record/8143632/files/figure.png" pageId="2" pageNumber="3">3A</figureCitation>
|
||
). Later these cells form numerous dendrites remaining close to ventral surface (
|
||
<figureCitation id="13632A7DFF9FFFBF98E086C23D77B48C" box="[1200,1319,1759,1783]" captionStart="Fig" captionStartId="3.[151,186,1505,1527]" captionTargetBox="[151,775,189,1482]" captionTargetId="figure-685@3.[151,775,188,1482]" captionTargetPageId="3" captionText="Fig. 3. Toluidine blue staining of Cercaria cattieni 1. A – cercarial embryo, lateral view, early stage of mucoid glands (mg) formation; B – cercarial embryos at different stage, metachromatic staining shows mucoid glands; C – later cercarial embryo (tail lost during fixation), metachromatic staining of mucoid glands with numerous dendrites; D – mucoid staining of stylet pouch; E – mucoid on the surface of the fore region; F, G – mucoid in the midbody, beneath musculature and tegument; ventral nerve chords (vnc) are visible among mucoid; H – midbody, mucoid transferred to the tegument. os – oral sucker; vs – ventral sucker; t – tail. A, D, E, G, H – differential interference contrast." figureDoi="http://doi.org/10.5281/zenodo.8143632" httpUri="https://zenodo.org/record/8143632/files/figure.png" pageId="2" pageNumber="3">Fig. 3B, C</figureCitation>
|
||
). At a certain stage, part of mucoid transferred into region of stylet pouch (
|
||
<figureCitation id="13632A7DFF9FFFBF9F2F87023B86B54C" box="[895,982,1823,1847]" captionStart="Fig" captionStartId="3.[151,186,1505,1527]" captionTargetBox="[151,775,189,1482]" captionTargetId="figure-685@3.[151,775,188,1482]" captionTargetPageId="3" captionText="Fig. 3. Toluidine blue staining of Cercaria cattieni 1. A – cercarial embryo, lateral view, early stage of mucoid glands (mg) formation; B – cercarial embryos at different stage, metachromatic staining shows mucoid glands; C – later cercarial embryo (tail lost during fixation), metachromatic staining of mucoid glands with numerous dendrites; D – mucoid staining of stylet pouch; E – mucoid on the surface of the fore region; F, G – mucoid in the midbody, beneath musculature and tegument; ventral nerve chords (vnc) are visible among mucoid; H – midbody, mucoid transferred to the tegument. os – oral sucker; vs – ventral sucker; t – tail. A, D, E, G, H – differential interference contrast." figureDoi="http://doi.org/10.5281/zenodo.8143632" httpUri="https://zenodo.org/record/8143632/files/figure.png" pageId="2" pageNumber="3">Fig. 3D</figureCitation>
|
||
). Next, metachromatic staining disappears from stylet pouch, being found on cercarial surface (
|
||
<figureCitation id="13632A7DFF9FFFBF992287223B1EB50C" captionStart="Fig" captionStartId="3.[151,186,1505,1527]" captionTargetBox="[151,775,189,1482]" captionTargetId="figure-685@3.[151,775,188,1482]" captionTargetPageId="3" captionText="Fig. 3. Toluidine blue staining of Cercaria cattieni 1. A – cercarial embryo, lateral view, early stage of mucoid glands (mg) formation; B – cercarial embryos at different stage, metachromatic staining shows mucoid glands; C – later cercarial embryo (tail lost during fixation), metachromatic staining of mucoid glands with numerous dendrites; D – mucoid staining of stylet pouch; E – mucoid on the surface of the fore region; F, G – mucoid in the midbody, beneath musculature and tegument; ventral nerve chords (vnc) are visible among mucoid; H – midbody, mucoid transferred to the tegument. os – oral sucker; vs – ventral sucker; t – tail. A, D, E, G, H – differential interference contrast." figureDoi="http://doi.org/10.5281/zenodo.8143632" httpUri="https://zenodo.org/record/8143632/files/figure.png" pageId="2" pageNumber="3">Fig. 3E</figureCitation>
|
||
). Mucoid from rest of glands transferred into body tegument, mostly in middle third of body (
|
||
<figureCitation id="13632A7DFF9FFFBF988287623D15B5EC" box="[1234,1349,1919,1943]" captionStart="Fig" captionStartId="3.[151,186,1505,1527]" captionTargetBox="[151,775,189,1482]" captionTargetId="figure-685@3.[151,775,188,1482]" captionTargetPageId="3" captionText="Fig. 3. Toluidine blue staining of Cercaria cattieni 1. A – cercarial embryo, lateral view, early stage of mucoid glands (mg) formation; B – cercarial embryos at different stage, metachromatic staining shows mucoid glands; C – later cercarial embryo (tail lost during fixation), metachromatic staining of mucoid glands with numerous dendrites; D – mucoid staining of stylet pouch; E – mucoid on the surface of the fore region; F, G – mucoid in the midbody, beneath musculature and tegument; ventral nerve chords (vnc) are visible among mucoid; H – midbody, mucoid transferred to the tegument. os – oral sucker; vs – ventral sucker; t – tail. A, D, E, G, H – differential interference contrast." figureDoi="http://doi.org/10.5281/zenodo.8143632" httpUri="https://zenodo.org/record/8143632/files/figure.png" pageId="2" pageNumber="3">Fig. 3F–H</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BE736F8FF9FFFBE9F1B87BD3BEAB30C" blockId="2.[812,1438,1279,2071]" lastBlockId="3.[812,1437,191,375]" lastPageId="3" lastPageNumber="4" pageId="2" pageNumber="3">
|
||
We obtained sequences of three fragments of the rDNA and the mitochondrial COI gene for
|
||
<taxonomicName id="4C584D7BFF9FFFBF98FD87DD3D4BB5AC" box="[1197,1307,1984,2007]" class="Trematoda" family="Aporocotylidae" genus="Cercaria" kingdom="Animalia" order="Diplostomida" pageId="2" pageNumber="3" phylum="Platyhelminthes" rank="species" species="cattieni">
|
||
<emphasis id="B92CEAEAFF9FFFBF98FD87DD3D4BB5AC" box="[1197,1307,1984,2007]" italics="true" pageId="2" pageNumber="3">C. cattieni</emphasis>
|
||
</taxonomicName>
|
||
1 (
|
||
<tableCitation id="C6DA0343FF9FFFBF996D87A23DDCB5AC" box="[1341,1420,1983,2007]" captionStart="Table 1" captionStartId="1.[151,207,189,210]" captionTargetPageId="1" captionText="Table 1. DNA sequences obtained for Cercaria cattieni 1." pageId="2" pageNumber="3">Table 1</tableCitation>
|
||
). None of these sequences had any close BLAST hits. Preliminary 28S rDNA-based phylogenetic analysis (covering a broad range of taxa within the Xiphidiata) suggested focusing the dataset on the
|
||
<taxonomicName id="4C584D7BFF9EFFBE9DF787423A07B50C" authorityName="Ward" authorityYear="1901" box="[423,599,1887,1911]" pageId="3" pageNumber="2" rank="superFamily" superFamily="Microphalloidea">Microphalloidea</taxonomicName>
|
||
and particularly the
|
||
<taxonomicName id="4C584D7BFF9EFFBE9CEF87623912B5EC" authorityName="Dollfus" authorityYear="1939" box="[191,322,1919,1943]" class="Trematoda" family="Renicolidae" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="family">Renicolidae</taxonomicName>
|
||
. The final alignment included 40 sequences and was 1,226 bp long, including 39 gaps. The resulting phylogenetic tree (
|
||
<figureCitation id="13632A7DFF9EFFBE9DC287A23985B5AC" box="[402,469,1983,2007]" captionStart="Fig" captionStartId="4.[151,186,1328,1350]" captionTargetBox="[292,1297,204,1285]" captionTargetId="figure-225@4.[284,1303,189,1298]" captionTargetPageId="4" captionText="Fig. 4. Phylogenetic position of Cercaria cattieni 1 within the Microphalloidea based on 28S rDNA sequence data analysis with Bayes- ian inference. Posterior probabilities are printed next to nodes; scale bar shows substitution rate." figureDoi="http://doi.org/10.5281/zenodo.8143634" httpUri="https://zenodo.org/record/8143634/files/figure.png" pageId="3" pageNumber="4">Fig. 4</figureCitation>
|
||
) featured two major clades corresponding to the
|
||
<taxonomicName id="4C584D7BFF9EFFBE9D2687C23A76B58C" authorityName="Ward" authorityYear="1901" box="[374,550,2015,2039]" pageId="3" pageNumber="2" rank="superFamily" superFamily="Microphalloidea">Microphalloidea</taxonomicName>
|
||
and Plagiorchioidea. One of the lineages within the
|
||
<taxonomicName id="4C584D7BFF9EFFBE9DA087E23AF0BA6C" authorityName="Ward" authorityYear="1901" box="[496,672,2047,2071]" pageId="3" pageNumber="2" rank="superFamily" superFamily="Microphalloidea">Microphalloidea</taxonomicName>
|
||
included the families
|
||
<taxonomicName id="4C584D7BFF9EFFBE9FFF80A23C61B2AC" authorityName="Dollfus" authorityYear="1939" box="[943,1073,191,215]" class="Trematoda" family="Renicolidae" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="family">Renicolidae</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C584D7BFF9EFFBE986B80A23CEDB2AC" authorityName="Skrjabin" authorityYear="1924" box="[1083,1213,191,215]" class="Trematoda" family="Eucotylidae" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="family">Eucotylidae</taxonomicName>
|
||
, the unknown family with unidentified cercariae from
|
||
<collectingCountry id="F34F7668FF9EFFBE98C480C23CB4B28C" box="[1172,1252,223,247]" name="Nigeria" pageId="3" pageNumber="4">Nigeria</collectingCountry>
|
||
(KX022508 and KX022509,
|
||
<bibRefCitation id="EFC94B09FF9EFFBE9FE580E23D76B36C" author="AWHARITOMA A. O. & ENABULELE E. E." box="[949,1318,255,279]" pageId="3" pageNumber="4" pagination="189 - 193" refId="ref3466" refString="AWHARITOMA A. O., ENABULELE E. E. 2018: Molecular phylogenetic location of a digenean larva isolated from the African ampullariid snail, Pila ovata, from Obazuwa in Edo State, Nigeria. Nigerian J. Parasitol. 39: 189 - 193." type="journal article" year="2018">Awharitoma and Enabulele 2018</bibRefCitation>
|
||
), and also
|
||
<taxonomicName id="4C584D7BFF9EFFBE9F7D813D3BCCB34C" box="[813,924,288,311]" class="Trematoda" family="Aporocotylidae" genus="Cercaria" kingdom="Animalia" order="Diplostomida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="species" species="cattieni">
|
||
<emphasis id="B92CEAEAFF9EFFBE9F7D813D3BCCB34C" box="[813,924,288,311]" italics="true" pageId="3" pageNumber="4">C. cattieni</emphasis>
|
||
</taxonomicName>
|
||
1 forming a well-supported clade with
|
||
<taxonomicName id="4C584D7BFF9EFFBE991481023CC5B32C" authority="(Rudolphi, 1819)" baseAuthorityName="Rudolphi" baseAuthorityYear="1819" class="Trematoda" family="Pachypsolidae" genus="Pachypsolus" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="species" species="irroratus">
|
||
<emphasis id="B92CEAEAFF9EFFBE991481023B9DB32C" italics="true" pageId="3" pageNumber="4">Pachypsolus irroratus</emphasis>
|
||
(Rudolphi, 1819)
|
||
</taxonomicName>
|
||
(family
|
||
<taxonomicName id="4C584D7BFF9EFFBE98A981223DC5B32C" authorityName="Yamaguti" authorityYear="1958" box="[1273,1429,319,343]" class="Trematoda" family="Pachypsolidae" kingdom="Animalia" order="Plagiorchiida" pageId="3" pageNumber="4" phylum="Platyhelminthes" rank="family">Pachypsolidae</taxonomicName>
|
||
) (AY222274).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |