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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.75.57192" ID-GBIF-Dataset="70ebf204-5c89-4782-8357-853357218fdd" ID-PMC="PMC7669824" ID-Pensoft-Pub="1314-4049-75-31" ID-Pensoft-UUID="C7E17CB78AA959F4AC1E9E9A425599B2" ID-PubMed="33223920" ModsDocID="1314-4049-75-31" checkinTime="1604986494284" checkinUser="pensoft" docAuthor="Lv, Shi-Long, Chai, Chun-Yue, Wang, Yun, Yan, Zhen-Li &amp; Hui, Feng-Li" docDate="2020" docId="B22E2D63311B5F2488AF69FFDE522A0F" docLanguage="en" docName="MycoKeys 75: 31-49" docOrigin="MycoKeys 75" docSource="http://dx.doi.org/10.3897/mycokeys.75.57192" docTitle="Spathaspora parajiuxiensis C. Y. Chai &amp; F. L. Hui 2020, sp. nov." docType="treatment" docVersion="4" id="C7E17CB78AA959F4AC1E9E9A425599B2" lastPageNumber="31" masterDocId="C7E17CB78AA959F4AC1E9E9A425599B2" masterDocTitle="Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China" masterLastPageNumber="49" masterPageNumber="31" pageNumber="31" updateTime="1668136577485" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Lv, Shi-Long</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chai, Chun-Yue</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Yun</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yan, Zhen-Li</mods:namePart>
<mods:affiliation>State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co. Ltd., Nanyang 473000, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hui, Feng-Li</mods:namePart>
<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
<mods:nameIdentifier type="email">fenglihui@yeah.net</mods:nameIdentifier>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2020</mods:date>
<mods:detail type="volume">
<mods:number>75</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>31</mods:start>
<mods:end>49</mods:end>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.75.57192</mods:url>
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<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.75.57192</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-75-31</mods:identifier>
<mods:identifier type="Pensoft-UUID">C7E17CB78AA959F4AC1E9E9A425599B2</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="168961851" LSID="urn:lsid:plazi:treatment:B22E2D63311B5F2488AF69FFDE522A0F" httpUri="http://treatment.plazi.org/id/B22E2D63311B5F2488AF69FFDE522A0F" lastPageNumber="31" pageId="0" pageNumber="31">
<subSubSection pageId="0" pageNumber="31" type="nomenclature">
<paragraph pageId="0" pageNumber="31">
<taxonomicName LSID="B22E2D63-311B-5F24-88AF-69FFDE522A0F" authority="C. Y. Chai &amp; F. L. Hui" authorityName="C. Y. Chai &amp; F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Debaryomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora parajiuxiensis" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="parajiuxiensis" status="sp. nov.">Spathaspora parajiuxiensis C.Y. Chai &amp; F.L. Hui</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="31">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5 % ME agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure5" httpUri="https://binary.pensoft.net/fig/473340" pageId="0" pageNumber="31">836447 Figure 5</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="31" type="materials_examined">
<paragraph pageId="0" pageNumber="31">Type.</paragraph>
<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2016, R.C. Ren &amp; L. Zhang (holotype, NYNU 16747T preserved in a metabolically-inactive state), ex-holotype: CICC 33162; CBS 14691.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="31" type="etymology">
<paragraph pageId="0" pageNumber="31">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="31">
<taxonomicName genus="Paraluxiensis" lsidName="Paraluxiensis" pageId="0" pageNumber="31" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="31">Paraluxiensis</emphasis>
</taxonomicName>
refers to its close phylogenetic relationship to
<taxonomicName lsidName="Sp. luxiensis" pageId="0" pageNumber="31" rank="species" species="luxiensis">
<emphasis italics="true" pageId="0" pageNumber="31">Sp. luxiensis</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="31" type="description">
<paragraph pageId="0" pageNumber="31">Description.</paragraph>
<paragraph pageId="0" pageNumber="31">
In YM broth after 3 days at 25 °C, cells are ovoid to elongate (3.5-4
<normalizedToken originalValue="×">x</normalizedToken>
7-15
<normalizedToken originalValue="μm">μm</normalizedToken>
) and occur singly or in pairs (Fig.
<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5 % ME agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure5" httpUri="https://binary.pensoft.net/fig/473340" pageId="0" pageNumber="31">5a</figureCitation>
); pseudohyphae are present. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. After 3 days of growth on YM agar at 25 °C, colonies are white to cream-coloured, butyrous and smooth with entire margins. After 12 days at 25 °C on Dalmau plate culture on CM agar, pseudohyphae and true hyphae are formed (Fig.
<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5 % ME agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure5" httpUri="https://binary.pensoft.net/fig/473340" pageId="0" pageNumber="31">5b</figureCitation>
). Sporulation occurs on 5% ME agar after 14 days at 25 °C. Unconjugated asci are formed from single cells with one elongated ascospores which are tapered and curved at the ends (Fig.
<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Morphology of Spathaspora parajiuxiensis (NYNU 16747, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d c ascus and ascospore (arrow) on 5 % ME agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure5" httpUri="https://binary.pensoft.net/fig/473340" pageId="0" pageNumber="31">5c</figureCitation>
) Glucose and maltose are weakly fermented. Xylose fermentation is negative using Durham tubes, but ethanol is produced from xylose when determined with alcohol oxidase and peroxidase tests. Glucose, d-glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, methyl
<normalizedToken originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
, cellobiose, salicin, arbutin, melezitose, inulin, ribitol, d-glucitol, d-mannitol, 2-keto-d-gluconate, succinate and ethanol are assimilated. No growth occurs with galactose, l-sorbose, l-arabinose, d-arabinose, l-rhamnose, melibiose, lactose, raffinose, glycerol, erythritol, xylitol, galactitol,
<emphasis italics="true" pageId="0" pageNumber="31">myo</emphasis>
-inositol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate, citrate, l-arabinitol or methanol. For the assimilation of nitrogen compounds, growth on l-lysine, glucosamine or d-tryptophan is present, whereas growth on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine or imidazole is absent. Growth is observed at 37 °C, but not at 40 °C. Growth in the presence of 0.01% cycloheximide is present, but growth in the presence of 0.1% cycloheximide, 10% NaCl plus 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.
</paragraph>
<caption doi="10.3897/mycokeys.75.57192.figure5" httpUri="https://binary.pensoft.net/fig/473340" pageId="0" pageNumber="31" start="Figure 5" startId="F5">
<paragraph pageId="0" pageNumber="31">
<emphasis bold="true" pageId="0" pageNumber="31">Figure 5.</emphasis>
Morphology of
<taxonomicName authorityName="C. Y. Chai &amp; F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Saccharomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora parajiuxiensis" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="parajiuxiensis">
<emphasis italics="true" pageId="0" pageNumber="31">Spathaspora parajiuxiensis</emphasis>
</taxonomicName>
(NYNU 16747, holotype)
<emphasis bold="true" pageId="0" pageNumber="31">a</emphasis>
budding cells and pseudohyphae on YM broth after 3 d
<emphasis bold="true" pageId="0" pageNumber="31">b</emphasis>
true hyphae with blastoconidia on CM agar after 14 d
<emphasis bold="true" pageId="0" pageNumber="31">c</emphasis>
ascus and ascospore (arrow) on 5% ME agar after 14 d. Scale bars: 10
<normalizedToken originalValue="μm">μm</normalizedToken>
.
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="31" type="additional isolate examined">
<paragraph pageId="0" pageNumber="31">Additional isolate examined.</paragraph>
<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2016, R.C. Ren &amp; L. Zhang, NYNU 16632.</paragraph>
</subSubSection>
</treatment>
</document>