284 lines
37 KiB
XML
284 lines
37 KiB
XML
<document id="36B8B8143151A0CCD061769A4631B9A9" ID-CLB-Dataset="6144" ID-DOI="10.11646/phytotaxa.39.1.1" ID-GBIF-Dataset="836e6558-6d05-4ad0-8a82-d11b7c8f9b37" ID-ISSN="1179-3163" ID-Zenodo-Dep="4894684" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1622651092293" checkinUser="felipe" docAuthor="Novis, Phil M. & Visnovsky, Gabriel" docDate="2012" docId="0391A5712105C66778ADF9764EA4FD14" docLanguage="en" docName="Phytotaxa.39.1-30.pdf.imf" docOrigin="Phytotaxa 39" docSource="http://biotaxa.org/Phytotaxa/article/view/phytotaxa.39.1.1" docStyle="DocumentStyle:13F189B31CB4CCCE9197361A4E100104.3:Phytotaxa.2011-2013.journal_article" docStyleId="13F189B31CB4CCCE9197361A4E100104" docStyleName="Phytotaxa.2011-2013.journal_article" docStyleVersion="3" docTitle="Schizochlamydella orbicularis Novis & Visnovsky 2012, sp. nov." docType="treatment" docVersion="7" lastPageNumber="22" masterDocId="FFA8DD092116C672783AFC0F4F7FFFE2" masterDocTitle="Novel alpine algae from New Zealand: Chlorophyta" masterLastPageNumber="30" masterPageNumber="1" pageNumber="20" updateTime="1698998801772" updateUser="ExternalLinkService" zenodo-license-document="CLOSED">
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<mods:titleInfo id="FE3074F29FA72AFB9CA8528CE0FADF40">
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<mods:title id="E47BB17949DC1842D9B203E4D19D2AFE">Novel alpine algae from New Zealand: Chlorophyta</mods:title>
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<mods:name id="7C41899CD90EE579D0C9661520FB65E5" type="personal">
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<mods:namePart id="923ED978E942B109771443BD7CE8562C">Novis, Phil M.</mods:namePart>
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<mods:affiliation id="15AA2F5F7BB203A9BBC478047BB07AEC">Allan Herbarium, Landcare Research, PO Box 40, Lincoln 7640, New Zealand; email: novisp @ landcareresearch. co. nz Department of Chemical and Process Engineering, University of Canterbury, Private Bag 4800, Christchurch, New Zealand</mods:affiliation>
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<mods:roleTerm id="B7EE36398957271A81C45DD2BFB1C1FC">Author</mods:roleTerm>
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<mods:namePart id="C8584C8CC3A800EF0443B12F81CB4781">Visnovsky, Gabriel</mods:namePart>
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<mods:typeOfResource id="935F3E26FDDC4B36DFAFDA3960629A93">text</mods:typeOfResource>
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<mods:title id="B69DCED8447E4DE9868D06129062EA4B">Phytotaxa</mods:title>
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<mods:part id="5C54F41AF50DB4E2D7FF1276B72FA8D1">
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<mods:date id="7C285CACFA16181BB9BD32A37380627F">2012</mods:date>
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<mods:number id="51C5F8C524FB8F5750EE873E27E75A04">2012-01-01</mods:number>
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<mods:number id="AD6DDFFEB982657E06897CDA72846598">39</mods:number>
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<mods:url id="C02D54B2684146590D0E1A2F706A46EC">http://biotaxa.org/Phytotaxa/article/view/phytotaxa.39.1.1</mods:url>
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<mods:classification id="A600B2088FCE54917031566A918B6BB7">journal article</mods:classification>
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<treatment id="0391A5712105C66778ADF9764EA4FD14" ID-DOI="http://doi.org/10.5281/zenodo.4926555" ID-GBIF-Taxon="182651462" ID-Zenodo-Dep="4926555" LSID="urn:lsid:plazi:treatment:0391A5712105C66778ADF9764EA4FD14" httpUri="http://treatment.plazi.org/id/0391A5712105C66778ADF9764EA4FD14" lastPageId="21" lastPageNumber="22" pageId="19" pageNumber="20">
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<subSubSection id="C32247EC2105C66178ADF9764C90FA70" box="[151,1007,1400,1427]" pageId="19" pageNumber="20" type="nomenclature">
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<paragraph id="8B8714672105C66178ADF9764C90FA70" blockId="19.[151,1007,1400,1427]" box="[151,1007,1400,1427]" pageId="19" pageNumber="20">
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<heading id="D0CFA30B2105C66178ADF9764C90FA70" bold="true" box="[151,1007,1400,1427]" fontSize="10" level="2" pageId="19" pageNumber="20" reason="2">
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<emphasis id="B94CC8752105C66178ADF9764C90FA70" bold="true" box="[151,1007,1400,1427]" pageId="19" pageNumber="20">
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<taxonomicName id="4C386FE42105C66178ADF9764D90FA70" authority="Novis & Visnovsky" authorityName="Novis & Visnovsky" authorityYear="2012" box="[151,751,1400,1427]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="19" pageNumber="20" phylum="Chlorophyta" rank="species" species="orbicularis" status="sp. nov.">
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<emphasis id="B94CC8752105C66178ADF9764E82FA71" bold="true" box="[151,509,1401,1427]" italics="true" pageId="19" pageNumber="20">Schizochlamydella orbicularis</emphasis>
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Novis & Visnovsky
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</taxonomicName>
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,
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<emphasis id="B94CC8752105C6617AC6F9764C29FA71" bold="true" box="[764,854,1401,1427]" italics="true" pageId="19" pageNumber="20">
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<taxonomicNameLabel id="A27F750E2105C6617AC6F9764C29FA71" box="[764,854,1401,1427]" pageId="19" pageNumber="20" rank="species">sp. nov.</taxonomicNameLabel>
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||
</emphasis>
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||
(
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<figureCitation id="130308E22105C6617B5FF9774C98FA70" box="[869,999,1400,1426]" captionStart="FIGURE 4" captionStartId="20.[151,245,1617,1638]" captionTargetBox="[151,1436,181,1591]" captionTargetId="figure-191@20.[151,1436,181,1592]" captionTargetPageId="20" captionText="FIGURE 4. A–C. Achoma brachiatum (photographed from culture LCR-CG11). A. Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp). B, C, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix. D, E Schizochlamydella orbicularis (photographed from culture LCR-CG9). D. Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow). E. Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in D (use for A and D), 1 µm in B, C, and E." figureDoi="http://doi.org/10.5281/zenodo.4894692" httpUri="https://zenodo.org/record/4894692/files/figure.png" pageId="19" pageNumber="20">Figs 4D–E</figureCitation>
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)
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</emphasis>
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||
</heading>
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||
</paragraph>
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||
</subSubSection>
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<subSubSection id="C32247EC2105C66178ADF9CF4B12F9D5" pageId="19" pageNumber="20" type="diagnosis">
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<paragraph id="8B8714672105C66178ADF9CF4B12F9D5" blockId="19.[151,1436,1472,1591]" pageId="19" pageNumber="20">
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<emphasis id="B94CC8752105C66178ADF9CF4B12F9D5" italics="true" pageId="19" pageNumber="20">
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Cellulae sphaericae vel subsphaericae,
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<quantity id="4CC0B9822105C6617A6AF9CF4DB3FA35" box="[592,716,1472,1495]" metricMagnitude="-3" metricUnit="m" metricValue="4.6" metricValueMax="6.1" metricValueMin="3.1" pageId="19" pageNumber="20" unit="mm" value="4.6" valueMax="6.1" valueMin="3.1">3.1–6.1 µm</quantity>
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latae. Paries cellularis tenuis laevisque.
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<taxonomicName id="4C386FE42105C6617C8AF9CF4AE8FA35" box="[1200,1431,1472,1495]" class="Trebouxiophyceae" family="Chlorellaceae" genus="Chloroplastus" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" order="Chlorellales" pageId="19" pageNumber="20" phylum="Chlorophyta" rank="species" species="viridis">Chloroplastus viridis</taxonomicName>
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, parietalis, lobatus, in cellulis valentibus incisuris 2–4 instructus. Pyrenoides nulla. Cellulae coloniales, mucilaginae maturae per matricem mucilaginis achromaticam instratosam uniformiter aequidistantes. Autosporis 2–4–8 per sporangium regenerans, eae per expansionem matricis gradatim separantes.
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</emphasis>
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</paragraph>
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</subSubSection>
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<subSubSection id="C32247EC2105C66178ADFA694DEAF945" pageId="19" pageNumber="20" type="materials_examined">
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<paragraph id="8B8714672105C66178ADFA694DEAF945" blockId="19.[151,1436,1638,2012]" pageId="19" pageNumber="20">
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<materialsCitation id="3B501E3A2105C66178ADFA694DEFF945" ID-GBIF-Occurrence="3128888302" collectingDate="2007-11-30" collectionCode="CHR" country="New Zealand" elevation="1400" location="Westland" municipality="Mt Philistine" pageId="19" pageNumber="20" specimenCode="CHR610488" specimenCount="1" typeStatus="holotype">
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<emphasis id="B94CC8752105C66178ADFA694F80F962" bold="true" box="[151,255,1638,1664]" pageId="19" pageNumber="20">
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<typeStatus id="5483AAC52105C66178ADFA694FA5F962" box="[151,218,1638,1664]" pageId="19" pageNumber="20">Type</typeStatus>
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:—
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||
</emphasis>
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||
<collectingCountry id="F32F54F72105C661793AFA694E9FF962" box="[256,480,1638,1664]" name="New Zealand" pageId="19" pageNumber="20">NEW ZEALAND</collectingCountry>
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:
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<location id="8EE742BC2105C66179D7FA694D1CF962" LSID="urn:lsid:plazi:treatment:0391A5712105C66778ADF9764EA4FD14:8EE742BC2105C66179D7FA694D1CF962" box="[493,611,1638,1664]" country="New Zealand" municipality="Mt Philistine" name="Westland" pageId="19" pageNumber="20">Westland</location>
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:
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||
<collectingMunicipality id="6BE38E1D2105C6617A4BFA694C68F962" box="[625,791,1638,1664]" pageId="19" pageNumber="20">Mt Philistine</collectingMunicipality>
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,
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||
<quantity id="4CC0B9822105C6617B1FFA694CFAF962" box="[805,901,1638,1664]" metricMagnitude="3" metricUnit="m" metricValue="1.4" pageId="19" pageNumber="20" unit="m" value="1400.0">
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<elevation id="0015F3542105C6617B1FFA694CFAF962" box="[805,901,1638,1664]" metricMagnitude="3" metricUnit="m" metricValue="1.4" pageId="19" pageNumber="20" unit="m" value="1400.0">1400 m</elevation>
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||
</quantity>
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||
, preserved cultured specimen from sample collected
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||
<date id="FF8632A72105C6617932FA824E93F945" box="[264,492,1677,1703]" pageId="19" pageNumber="20" value="2007-11-30">
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||
<collectingDate id="EFC2CB4F2105C6617932FA824E93F945" box="[264,492,1677,1703]" pageId="19" pageNumber="20" value="2007-11-30">30 November 2007</collectingDate>
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||
</date>
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||
,
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<specimenCode id="DB9EBC1C2105C66179C2FA824DEFF945" box="[504,656,1677,1703]" collectionCode="CHR" country="New Zealand" httpUri="http://grbio.org/cool/dbb8-zaka" name="Landcare Research New Zealand Limited" pageId="19" pageNumber="20" type="Herbarium">CHR610488</specimenCode>
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||
</materialsCitation>
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||
.
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||
</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C32247EC2105C66178FCFABB4D38F854" pageId="19" pageNumber="20" type="description">
|
||
<paragraph id="8B8714672105C66178FCFABB4C71F86D" blockId="19.[151,1436,1638,2012]" pageId="19" pageNumber="20">
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||
Cells colonial, spherical or near-spherical,
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||
<quantity id="4CC0B9822105C6617AEBFABB4C22F92C" box="[721,861,1716,1742]" metricMagnitude="-3" metricUnit="m" metricValue="4.6" metricValueMax="6.1" metricValueMin="3.1" pageId="19" pageNumber="20" unit="mm" value="4.6" valueMax="6.1" valueMin="3.1">3.1–6.1 µm</quantity>
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wide. Cell wall thin and smooth. Chloroplast green, parietal, lobed, with 2–4 incisions in healthy cells. Pyrenoid absent (
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<figureCitation id="130308E22105C6617C35FAD54B14F916" box="[1039,1131,1754,1780]" captionStart="FIGURE 4" captionStartId="20.[151,245,1617,1638]" captionTargetBox="[151,1436,181,1591]" captionTargetId="figure-191@20.[151,1436,181,1592]" captionTargetPageId="20" captionText="FIGURE 4. A–C. Achoma brachiatum (photographed from culture LCR-CG11). A. Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp). B, C, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix. D, E Schizochlamydella orbicularis (photographed from culture LCR-CG9). D. Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow). E. Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in D (use for A and D), 1 µm in B, C, and E." figureDoi="http://doi.org/10.5281/zenodo.4894692" httpUri="https://zenodo.org/record/4894692/files/figure.png" pageId="19" pageNumber="20">Fig. 4E</figureCitation>
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). Colonies mucilaginous, mature cells spaced evenly through colourless, unlayered mucilage matrix (
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<figureCitation id="130308E22105C6617C72FB0E4BD2F8F9" box="[1096,1197,1793,1819]" captionStart="FIGURE 4" captionStartId="20.[151,245,1617,1638]" captionTargetBox="[151,1436,181,1591]" captionTargetId="figure-191@20.[151,1436,181,1592]" captionTargetPageId="20" captionText="FIGURE 4. A–C. Achoma brachiatum (photographed from culture LCR-CG11). A. Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp). B, C, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix. D, E Schizochlamydella orbicularis (photographed from culture LCR-CG9). D. Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow). E. Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in D (use for A and D), 1 µm in B, C, and E." figureDoi="http://doi.org/10.5281/zenodo.4894692" httpUri="https://zenodo.org/record/4894692/files/figure.png" pageId="19" pageNumber="20">Fig. 4A</figureCitation>
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||
). Reproduction by autospores, 2–4–8 per sporangium, gradually separating through expansion of mucilage matrix (
|
||
<figureCitation id="130308E22105C6617D16FB274AF0F8A0" box="[1324,1423,1832,1858]" captionStart="FIGURE 4" captionStartId="20.[151,245,1617,1638]" captionTargetBox="[151,1436,181,1591]" captionTargetId="figure-191@20.[151,1436,181,1592]" captionTargetPageId="20" captionText="FIGURE 4. A–C. Achoma brachiatum (photographed from culture LCR-CG11). A. Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp). B, C, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix. D, E Schizochlamydella orbicularis (photographed from culture LCR-CG9). D. Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow). E. Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in D (use for A and D), 1 µm in B, C, and E." figureDoi="http://doi.org/10.5281/zenodo.4894692" httpUri="https://zenodo.org/record/4894692/files/figure.png" pageId="19" pageNumber="20">Fig. 4A</figureCitation>
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||
). Sequence data for both the
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<emphasis id="B94CC8752105C66179EEFB404E84F88A" box="[468,507,1871,1896]" italics="true" pageId="19" pageNumber="20">rbc</emphasis>
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L and 18S rDNA genes suggested that the species formed a novel lineage in the
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<taxonomicName id="4C386FE42105C66178ADFB7A4E0CF86D" box="[151,371,1909,1935]" class="Trebouxiophyceae" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" pageId="19" pageNumber="20" phylum="Chlorophyta" rank="class">Trebouxiophyceae</taxonomicName>
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, with no clear affinities (
|
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<figureCitation id="130308E22105C6617AACFB7A4D9EF86D" box="[662,737,1909,1935]" captionStart="FIGURE 8" captionStartId="26.[151,244,1780,1801]" captionTargetBox="[228,1347,203,1708]" captionTargetId="figure-69@26.[209,1378,197,1755]" captionTargetPageId="26" captionText="FIGURE 8. Phylogenetic analysis of the Trebouxiophyceae inferred from 18S rDNA sequences. Sequences contributed in this study are in bold. The tree typology is that inferred using MrBayes v3.0B4 (5 million generations). Numbers associated with nodes are Bayesian PP/MPB percentages (500 replicates). Scale bar represents 0.1 changes/site." figureDoi="http://doi.org/10.5281/zenodo.4894702" httpUri="https://zenodo.org/record/4894702/files/figure.png" pageId="19" pageNumber="20">Figs 8</figureCitation>
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||
,
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<figureCitation id="130308E22105C6617AD4FB7A4D80F86D" box="[750,767,1909,1935]" captionStart="FIGURE 9" captionStartId="27.[151,245,1913,1934]" captionTargetBox="[227,1378,194,1896]" captionTargetId="figure-77@27.[209,1378,191,1909]" captionTargetPageId="27" captionText="FIGURE 9. Phylogenetic analysis of a combined dataset representing the Chlorophyceae and Trebouxiophyceae inferred from rbcL gene sequences, with the third codon position omitted. Sequences contributed in this study are in bold. The tree typology is that inferred using MrBayes v3.0B4 (5 million generations). Numbers associated with nodes are Bayesian PP/MPB percentages (500 replicates). Scale bar represents 0.1 changes/site." figureDoi="http://doi.org/10.5281/zenodo.4894704" httpUri="https://zenodo.org/record/4894704/files/figure.png" pageId="19" pageNumber="20">9</figureCitation>
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).
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</paragraph>
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<paragraph id="8B8714672105C66178FCFB944D38F854" blockId="19.[151,1436,1638,2012]" box="[198,583,1947,1974]" pageId="19" pageNumber="20">
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||
<emphasis id="B94CC8752105C66178FCFB944E32F857" bold="true" box="[198,333,1947,1973]" pageId="19" pageNumber="20">Habitat:—</emphasis>
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Alpine herbfield soil.
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C32247EC2105C66178FCFBCD4D54F83E" box="[198,555,1986,2012]" pageId="19" pageNumber="20" type="distribution">
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<paragraph id="8B8714672105C66178FCFBCD4D54F83E" blockId="19.[151,1436,1638,2012]" box="[198,555,1986,2012]" pageId="19" pageNumber="20">
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||
<emphasis id="B94CC8752105C66178FCFBCD4EF9F83E" bold="true" box="[198,390,1986,2012]" pageId="19" pageNumber="20">Distribution:—</emphasis>
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||
<collectingCountry id="F32F54F72105C66179BCFBCD4D58F83E" box="[390,551,1986,2012]" name="New Zealand" pageId="19" pageNumber="20">New Zealand</collectingCountry>
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||
.
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||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="DF4744EF2102C66678ADFA5E4E31F8D6" ID-DOI="http://doi.org/10.5281/zenodo.4894692" ID-Zenodo-Dep="4894692" httpUri="https://zenodo.org/record/4894692/files/figure.png" pageId="20" pageNumber="21" startId="20.[151,245,1617,1638]" targetBox="[151,1436,181,1591]" targetPageId="20">
|
||
<paragraph id="8B8714672102C66678ADFA5E4B7DF95C" blockId="20.[151,1436,1617,1844]" pageId="20" pageNumber="21">
|
||
<emphasis id="B94CC8752102C66678ADFA5E4E34F984" bold="true" box="[151,331,1617,1638]" pageId="20" pageNumber="21">FIGURE 4. A–C</emphasis>
|
||
.
|
||
<taxonomicName id="4C386FE42102C6667962FA5D4D61F985" authorityName="Novis & Visnovsky" authorityYear="2012" box="[344,542,1618,1639]" class="Trebouxiophyceae" family="Chlorellaceae" genus="Achoma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" order="Chlorellales" pageId="20" pageNumber="21" phylum="Chlorophyta" rank="species" species="brachiatum">
|
||
<emphasis id="B94CC8752102C6667962FA5D4D61F985" box="[344,542,1618,1639]" italics="true" pageId="20" pageNumber="21">Achoma brachiatum</emphasis>
|
||
</taxonomicName>
|
||
(photographed from culture LCR-CG11).
|
||
<emphasis id="B94CC8752102C6667B85FA5E4CA8F984" bold="true" box="[959,983,1617,1638]" pageId="20" pageNumber="21">A.</emphasis>
|
||
Light micrograph of vegetative cells showing variable shape, prominent pyrenoid (pyr), and incised parietal chloroplast (cp).
|
||
<emphasis id="B94CC8752102C6667BACFA614CD9F961" bold="true" box="[918,934,1646,1667]" pageId="20" pageNumber="21">B</emphasis>
|
||
,
|
||
<emphasis id="B94CC8752102C6667B89FA614CBBF961" bold="true" box="[947,964,1646,1667]" pageId="20" pageNumber="21">C</emphasis>
|
||
, Transmission electron micrographs of zoospore showing mitochondria (mito), chloroplast containing starch (st) and prominent pyrenoid (pyr), components of flagellar basal apparatus (fba), absence of cell wall, and branching pattern of thylakoids (tm) in the pyrenoid matrix.
|
||
</paragraph>
|
||
<paragraph id="8B8714672102C66678ADFAC94E31F8D6" blockId="20.[151,1436,1617,1844]" pageId="20" pageNumber="21">
|
||
<emphasis id="B94CC8752102C66678ADFAC94FBAF939" bold="true" box="[151,197,1734,1755]" pageId="20" pageNumber="21">D, E</emphasis>
|
||
<taxonomicName id="4C386FE42102C66678F6FAC84E8AF93E" box="[204,501,1735,1756]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="20" pageNumber="21" phylum="Chlorophyta" rank="species" species="orbicularis">
|
||
<emphasis id="B94CC8752102C66678F6FAC84E8AF93E" box="[204,501,1735,1756]" italics="true" pageId="20" pageNumber="21">Schizochlamydella orbicularis</emphasis>
|
||
</taxonomicName>
|
||
(photographed from culture LCR-CG9).
|
||
<emphasis id="B94CC8752102C6667BAAFAC94CD7F939" bold="true" box="[912,936,1734,1755]" pageId="20" pageNumber="21">D.</emphasis>
|
||
Light micrograph of mature cells evenly spaced in mucilage, and newly released autospores (arrow).
|
||
<emphasis id="B94CC8752102C6667AA1FAEB4DCDF91B" bold="true" box="[667,690,1764,1785]" pageId="20" pageNumber="21">E.</emphasis>
|
||
Transmission electron micrograph of cell showing mitochondrion (mito), nucleus (nuc) cell wall (cw), and parietal chloroplast containing starch (st) but no pyrenoid. Scales: 10 µm in
|
||
<emphasis id="B94CC8752102C6667C85FB0E4BAFF8F4" bold="true" box="[1215,1232,1793,1814]" pageId="20" pageNumber="21">D</emphasis>
|
||
(use for
|
||
<emphasis id="B94CC8752102C6667D13FB0E4A45F8F4" bold="true" box="[1321,1338,1793,1814]" pageId="20" pageNumber="21">A</emphasis>
|
||
and
|
||
<emphasis id="B94CC8752102C6667D50FB0E4A04F8F4" bold="true" box="[1386,1403,1793,1814]" pageId="20" pageNumber="21">D</emphasis>
|
||
), 1 µm in
|
||
<emphasis id="B94CC8752102C66678ECFB114F99F8D1" bold="true" box="[214,230,1822,1843]" pageId="20" pageNumber="21">B</emphasis>
|
||
,
|
||
<emphasis id="B94CC8752102C66678C8FB114E7CF8D1" bold="true" box="[242,259,1822,1843]" pageId="20" pageNumber="21">C</emphasis>
|
||
, and
|
||
<emphasis id="B94CC8752102C6667902FB114E37F8D1" bold="true" box="[312,328,1822,1843]" pageId="20" pageNumber="21">E</emphasis>
|
||
.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="C32247EC2102C66678FCFB864C32F846" box="[198,845,1929,1956]" pageId="20" pageNumber="21" type="etymology">
|
||
<paragraph id="8B8714672102C66678FCFB864C32F846" blockId="20.[151,1436,1929,2033]" box="[198,845,1929,1956]" pageId="20" pageNumber="21">
|
||
<emphasis id="B94CC8752102C66678FCFB864E0BF841" bold="true" box="[198,372,1929,1955]" pageId="20" pageNumber="21">Etymology:—</emphasis>
|
||
“Spherical shaped”, referring to the cell.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C32247EC2102C66778FCFBBF4EA4FD14" lastPageId="21" lastPageNumber="22" pageId="20" pageNumber="21" type="discussion">
|
||
<paragraph id="8B8714672102C66778FCFBBF4D0CFD2D" blockId="20.[151,1436,1929,2033]" lastBlockId="21.[151,1436,152,758]" lastPageId="21" lastPageNumber="22" pageId="20" pageNumber="21">
|
||
<emphasis id="B94CC8752102C66678FCFBBF4EECF828" bold="true" box="[198,403,1968,1994]" pageId="20" pageNumber="21">Observations:—</emphasis>
|
||
The morphology of this species—mucilaginous colonies, lack of pyrenoids and zoospores—suggests an affinity with
|
||
<taxonomicName id="4C386FE42102C6667A6CFBD84DA0F812" box="[598,735,2007,2032]" class="Myxosporea" family="Myxidiidae" genus="Coccomyxa" kingdom="Animalia" order="Bivalvulida" pageId="20" pageNumber="21" phylum="Myxozoa" rank="genus">
|
||
<emphasis id="B94CC8752102C6667A6CFBD84DA0F812" box="[598,735,2007,2032]" italics="true" pageId="20" pageNumber="21">Coccomyxa</emphasis>
|
||
</taxonomicName>
|
||
, yet
|
||
<taxonomicName id="4C386FE42102C6667B21FBD84CC2F812" box="[795,957,2007,2032]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="20" pageNumber="21" phylum="Chlorophyta" rank="species" species="orbicularis">
|
||
<emphasis id="B94CC8752102C6667B21FBD84CC2F812" box="[795,957,2007,2032]" italics="true" pageId="20" pageNumber="21">S. orbicularis</emphasis>
|
||
</taxonomicName>
|
||
is clearly a distant relative of species in the
|
||
<taxonomicName id="4C386FE42103C66778FEFC974E30FF53" box="[196,335,152,177]" class="Myxosporea" family="Myxidiidae" genus="Coccomyxa" kingdom="Animalia" order="Bivalvulida" pageId="21" pageNumber="22" phylum="Myxozoa" rank="genus">
|
||
<emphasis id="B94CC8752103C66778FEFC974E30FF53" box="[196,335,152,177]" italics="true" pageId="21" pageNumber="22">Coccomyxa</emphasis>
|
||
</taxonomicName>
|
||
clades. More discussion of these clades is given in remarks on
|
||
<taxonomicName id="4C386FE42103C6677C61FC974AE3FF53" authorityName="Fott" authorityYear="1981" baseAuthorityName="Mainx" box="[1115,1436,152,177]" class="Trebouxiophyceae" family="Chlorellaceae" genus="Pseudococcomyxa" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" order="Chlorellales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="species" species="simplex">
|
||
<emphasis id="B94CC8752103C6677C61FC974AE3FF53" box="[1115,1436,152,177]" italics="true" pageId="21" pageNumber="22">Pseudococcomyxa simplex</emphasis>
|
||
</taxonomicName>
|
||
(below). The colonial mucilaginous habit lacking zoospores accords with the
|
||
<taxonomicName id="4C386FE42103C6677C7AFCB14E2EFF1D" authority="sensu Ettl & Gartner (1995)" authorityName="Ettl & Gartner" authorityYear="1995" class="Chlorophyceae" family="Radiococcaceae" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlamydomonadales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="family">
|
||
Radiococcaceae
|
||
<emphasis id="B94CC8752103C6677D32FCB04A35FF3A" box="[1288,1354,191,216]" italics="true" pageId="21" pageNumber="22">sensu</emphasis>
|
||
Ettl &
|
||
<bibRefCitation id="EFA969962103C66778ADFCEA4E2EFF1D" author="Gartner, G." box="[151,337,229,255]" pageId="21" pageNumber="22" refId="ref12305" refString="Ettl, H. & Gartner, G. (1995) Syllabus der Boden-, Luft-, und Flechtenalgen. Gustav Fischer Verlag, Stuttgart Jena & New York. 721 pp." type="book" year="1995">Gärtner (1995)</bibRefCitation>
|
||
</taxonomicName>
|
||
. Of the genera therein, the
|
||
<collectingCountry id="F32F54F72103C6677A97FCEA4C2EFF1D" box="[685,849,229,255]" name="New Zealand" pageId="21" pageNumber="22">New Zealand</collectingCountry>
|
||
strain most closely matches
|
||
<taxonomicName id="4C386FE42103C6677C8DFCEA4E0CFEC4" authority="Korshikov (1953)" authorityName="Korshikov" authorityYear="1953" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="genus">
|
||
<emphasis id="B94CC8752103C6677C8DFCEA4AE3FF1C" box="[1207,1436,229,254]" italics="true" pageId="21" pageNumber="22">Schizochlamydella</emphasis>
|
||
<bibRefCitation id="EFA969962103C66778ADFD034E0CFEC4" author="Korshikov, A. A." box="[151,371,268,294]" pageId="21" pageNumber="22" pagination="439" refId="ref12798" refString="Korshikov, A. A. (1953) Vtznachnyk prisnovodnykh vdorostej Ukrajns'koj RSR. V. Pidklas Protokokovi (Protococcineae). Vakuol'ni (Vacuolales) ta Protokokovi (Protococcales). Akad. Nauk URSR, Kiev, p. 439." type="book chapter" year="1953">Korshikov (1953)</bibRefCitation>
|
||
</taxonomicName>
|
||
, with cells irregularly dispersed in homogeneous mucilage. Only one other species of
|
||
<taxonomicName id="4C386FE42103C66778ADFD3D4E0CFEA9" authorityName="Korshikov" authorityYear="1953" box="[151,371,306,331]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="genus">
|
||
<emphasis id="B94CC8752103C66778ADFD3D4E0CFEA9" box="[151,371,306,331]" italics="true" pageId="21" pageNumber="22">Schizochlamydella</emphasis>
|
||
</taxonomicName>
|
||
lacks a pyrenoid, namely
|
||
<taxonomicName id="4C386FE42103C6677A93FD3D4B72FEAE" authority="Broady (1982)" authorityName="Broady" authorityYear="1982" box="[681,1037,306,332]" class="Chlorophyceae" family="Neochloridaceae" genus="Golenkinia" higherTaxonomySource="GBIF" kingdom="Plantae" order="Sphaeropleales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="species" species="minutissima">
|
||
<emphasis id="B94CC8752103C6677A93FD3D4C2AFEA9" box="[681,853,306,331]" italics="true" pageId="21" pageNumber="22">S. minutissima</emphasis>
|
||
<bibRefCitation id="EFA969962103C6677B67FD3D4B72FEAE" author="Broady, P. A." box="[861,1037,306,332]" pageId="21" pageNumber="22" pagination="445 - 484" refId="ref11844" refString="Broady, P. A. (1982) New records of Chlorophycean micro-algae cultured from Antarctic terrestrial habitats. Nova Hedwigia 36: 445 - 484." type="journal article" year="1982">Broady (1982)</bibRefCitation>
|
||
</taxonomicName>
|
||
. However, this species is slightly smaller than our strain, with ellipsoidal juvenile cells. One species has previously received attention at the molecular level (
|
||
<bibRefCitation id="EFA969962103C667794FFD8F4D2AFE78" author="Wolf, M. & Hepperle, D. & Krienitz, L." box="[373,597,384,410]" pageId="21" pageNumber="22" pagination="759 - 765" refId="ref13881" refString="Wolf, M., Hepperle, D. & Krienitz, L. (2003) On the phylogeny of Radiococcus, Planktosphaeria and Schizochlamydella (Radiococcaceae, Chlorophyta). Biologia 58: 759 - 765." type="journal article" year="2003">
|
||
Wolf
|
||
<emphasis id="B94CC8752103C66779FBFD8F4D7CFE7B" box="[449,515,384,409]" italics="true" pageId="21" pageNumber="22">et al.</emphasis>
|
||
2003
|
||
</bibRefCitation>
|
||
):
|
||
<taxonomicName id="4C386FE42103C6677A54FD8F4BA7FE78" authority="(Watanabe 1977)" baseAuthorityName="Watanabe" baseAuthorityYear="1977" box="[622,1240,384,410]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="species" species="capsulata">
|
||
<emphasis id="B94CC8752103C6677A54FD8F4C95FE7B" box="[622,1002,384,409]" italics="true" pageId="21" pageNumber="22">Schizochlamydella capsulata</emphasis>
|
||
(
|
||
<bibRefCitation id="EFA969962103C6677BC7FD8F4BB2FE78" author="Watanabe, S." box="[1021,1229,384,410]" pageId="21" pageNumber="22" pagination="338 - 343" refId="ref13792" refString="Watanabe, S. (1977) Schizochlamydella sphaerica sp. nov. (Chlorococcales) from Japanese soil. Journal of Japanese Botany 52: 338 - 343." type="journal article" year="1977">Watanabe 1977</bibRefCitation>
|
||
)
|
||
</taxonomicName>
|
||
. This strain is distinguished from our material by containing one or more pyrenoids, and is placed in the
|
||
<taxonomicName id="4C386FE42103C6677CFBFDA94A2AFE22" authorityName="K.H.Bohlin" authorityYear="1901" box="[1217,1365,422,448]" class="Chlorophyceae" family="Oocystaceae" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlamydomonadales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="family">Oocystaceae</taxonomicName>
|
||
using 18S rDNA sequences (
|
||
<bibRefCitation id="EFA969962103C6677992FDC24D0FFE05" author="Wolf, M. & Hepperle, D. & Krienitz, L." box="[424,624,461,487]" pageId="21" pageNumber="22" pagination="759 - 765" refId="ref13881" refString="Wolf, M., Hepperle, D. & Krienitz, L. (2003) On the phylogeny of Radiococcus, Planktosphaeria and Schizochlamydella (Radiococcaceae, Chlorophyta). Biologia 58: 759 - 765." type="journal article" year="2003">
|
||
Wolf
|
||
<emphasis id="B94CC8752103C66779D6FDC24D56FE04" box="[492,553,461,486]" italics="true" pageId="21" pageNumber="22">et al.</emphasis>
|
||
2003
|
||
</bibRefCitation>
|
||
), not closely related to
|
||
<taxonomicName id="4C386FE42103C6677BB3FDC24B53FE04" box="[905,1068,461,486]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="species" species="orbicularis">
|
||
<emphasis id="B94CC8752103C6677BB3FDC24B53FE04" box="[905,1068,461,486]" italics="true" pageId="21" pageNumber="22">S. orbicularis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="130308E22103C6677C07FDC24BF9FE05" box="[1085,1158,461,487]" captionStart="FIGURE 8" captionStartId="26.[151,244,1780,1801]" captionTargetBox="[228,1347,203,1708]" captionTargetId="figure-69@26.[209,1378,197,1755]" captionTargetPageId="26" captionText="FIGURE 8. Phylogenetic analysis of the Trebouxiophyceae inferred from 18S rDNA sequences. Sequences contributed in this study are in bold. The tree typology is that inferred using MrBayes v3.0B4 (5 million generations). Numbers associated with nodes are Bayesian PP/MPB percentages (500 replicates). Scale bar represents 0.1 changes/site." figureDoi="http://doi.org/10.5281/zenodo.4894702" httpUri="https://zenodo.org/record/4894702/files/figure.png" pageId="21" pageNumber="22">Fig. 8</figureCitation>
|
||
). Given the paucity of characters available to classify these species, it is not surprising that morphology does not reflect phylogeny. Typification in the genus
|
||
<taxonomicName id="4C386FE42103C66779EFFE154DC4FDD1" authorityName="Korshikov" authorityYear="1953" box="[469,699,538,563]" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="genus">
|
||
<emphasis id="B94CC8752103C66779EFFE154DC4FDD1" box="[469,699,538,563]" italics="true" pageId="21" pageNumber="22">Schizochlamydella</emphasis>
|
||
</taxonomicName>
|
||
is problematic, however. The
|
||
<typeStatus id="5483AAC52103C6677C07FE154B0EFDD6" box="[1085,1137,538,564]" pageId="21" pageNumber="22">type</typeStatus>
|
||
species of the genus,
|
||
<taxonomicName id="4C386FE42103C6677DBFFE154E0FFDB9" authority="(West)" baseAuthorityName="West" class="Chlorophyceae" family="Radiococcaceae" genus="Schizochlamydella" higherTaxonomySource="GBIF" kingdom="Plantae" order="Chlorococcales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="species" species="delicatula">
|
||
<emphasis id="B94CC8752103C6677DBFFE154E6AFDB8" italics="true" pageId="21" pageNumber="22">S. delicatula</emphasis>
|
||
(West)
|
||
</taxonomicName>
|
||
<bibRefCitation id="EFA969962103C6677940FE4E4D25FDB9" author="Korshikov, A. A." box="[378,602,577,603]" pageId="21" pageNumber="22" pagination="439" refId="ref12798" refString="Korshikov, A. A. (1953) Vtznachnyk prisnovodnykh vdorostej Ukrajns'koj RSR. V. Pidklas Protokokovi (Protococcineae). Vakuol'ni (Vacuolales) ta Protokokovi (Protococcales). Akad. Nauk URSR, Kiev, p. 439." type="book chapter" year="1953">Korshikov (1953)</bibRefCitation>
|
||
was transferred to the chrysophycean genus
|
||
<taxonomicName id="4C386FE42103C6677CA2FE4E4EF6FD60" authority="Lemmerman (1898)" authorityName="Lemmerman" authorityYear="1898" class="Phaeophyceae" family="Phaeothamniaceae" genus="Phaeoschizochlamys" higherTaxonomySource="GBIF" kingdom="Chromista" order="Phaeothamniales" pageId="21" pageNumber="22" phylum="Ochrophyta" rank="genus">
|
||
<emphasis id="B94CC8752103C6677CA2FE4E4AE3FDB8" box="[1176,1436,577,602]" italics="true" pageId="21" pageNumber="22">Phaeoschizochlamys</emphasis>
|
||
Lemmerman (1898)
|
||
</taxonomicName>
|
||
by
|
||
<bibRefCitation id="EFA969962103C667798DFE674DF8FD60" author="Bourrelly, P." box="[439,647,616,642]" pageId="21" pageNumber="22" pagination="1 - 412" refId="ref11983" refString="Bourrelly, P. (1957) Recherches sur les Chrysophycees. Morphologie, phylogenie, systematique. Revue Algologique, Nouvelle Serie 3: 1 - 412." type="journal article" year="1957">Bourrelly (1957)</bibRefCitation>
|
||
. However, this transfer was made without pigment or molecular analyses; we regard the choice of
|
||
<taxonomicName id="4C386FE42103C6677A1CFE804DACFD4A" box="[550,723,655,680]" class="Chlorophyceae" family="Neochloridaceae" genus="Schizochlamys" higherTaxonomySource="IPNI" kingdom="Plantae" order="Sphaeropleales" pageId="21" pageNumber="22" phylum="Chlorophyta" rank="genus">
|
||
<emphasis id="B94CC8752103C6677A1CFE804DACFD4A" box="[550,723,655,680]" italics="true" pageId="21" pageNumber="22">Schizochlamys</emphasis>
|
||
</taxonomicName>
|
||
for our material as prudent until the phylogenetic placement of the
|
||
<typeStatus id="5483AAC52103C66778D8FEBA4E6BFD2D" box="[226,276,693,719]" pageId="21" pageNumber="22">type</typeStatus>
|
||
species is clearly established.
|
||
</paragraph>
|
||
<paragraph id="8B8714672103C66778FCFED44EA4FD14" blockId="21.[151,1436,152,758]" box="[198,475,731,758]" pageId="21" pageNumber="22">
|
||
<emphasis id="B94CC8752103C66778FCFED44E25FD17" bold="true" box="[198,346,731,757]" pageId="21" pageNumber="22">Cultures:—</emphasis>
|
||
LCR-CG9.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |