211 lines
17 KiB
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211 lines
17 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.78.60878" ID-GBIF-Dataset="07ad5422-fc25-45dd-be81-03cb609ac30e" ID-PMC="PMC7910272" ID-Pensoft-Pub="1314-4049-78-49" ID-Pensoft-UUID="5BCC2C78F2F856C3B93134F805790F02" ID-PubMed="33664613" ModsDocID="1314-4049-78-49" checkinTime="1613833639718" checkinUser="pensoft" docAuthor="Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Sun, Wenxiu" docDate="2021" docId="F77900E1889B5291AEC8995A46C92870" docLanguage="en" docName="MycoKeys 78: 49-77" docOrigin="MycoKeys 78" docPubDate="2021-02-19" docSource="http://dx.doi.org/10.3897/mycokeys.78.60878" docTitle="Diaporthe middletonii R. G. Shivas, L. Morin, S. M. Thomps. & Y. P. Tan, Persoonia 35: 45. (2015)" docType="treatment" docVersion="4" id="5BCC2C78F2F856C3B93134F805790F02" lastPageNumber="49" masterDocId="5BCC2C78F2F856C3B93134F805790F02" masterDocTitle="Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China" masterLastPageNumber="77" masterPageNumber="49" pageNumber="49" updateTime="1668136623939" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-02-19</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>78</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>49</mods:start>
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<mods:end>77</mods:end>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.78.60878</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.78.60878</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-78-49</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5BCC2C78F2F856C3B93134F805790F02</mods:identifier>
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<treatment ID-GBIF-Taxon="177488917" LSID="urn:lsid:plazi:treatment:F77900E1889B5291AEC8995A46C92870" httpUri="http://treatment.plazi.org/id/F77900E1889B5291AEC8995A46C92870" lastPageNumber="49" pageId="0" pageNumber="49">
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<subSubSection pageId="0" pageNumber="49" type="nomenclature">
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName LSID="F77900E1-889B-5291-AEC8-995A46C92870" authority="R. G. Shivas, L. Morin, S. M. Thomps. & Y. P. Tan, Persoonia 35: 45. (2015)" authorityName="R. G. Shivas, L. Morin, S. M. Thomps. & Y. P. Tan, Persoonia 35: 45. (2015)" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe middletonii" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="middletonii">Diaporthe middletonii R.G. Shivas, L. Morin, S.M. Thomps. & Y.P. Tan, Persoonia 35: 45. (2015)</taxonomicName>
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<figureCitation captionStart="Figure 6" captionStartId="F8" captionText="Figure 6. Diaporthe middletonii (SAUCC 194.46) a infected leaf of Lithocarpus glaber b, c surface and reverse of colony after 15 days on PDA d, e conidiomata f-i conidiophores and conidiogenous cells j beta conidia k, l alpha conidia and beta conidia. Scale bars: 10 μm (f-l)." figureDoi="10.3897/mycokeys.78.60878.figure6" httpUri="https://binary.pensoft.net/fig/511681" pageId="0" pageNumber="49">Figure 6</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Description.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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Asexual morph: Leaf spots discoid to irregular. Conidiomata pycnidial, scattered or aggregated in groups of 3-5 pycnidia, globose, black, erumpent, coated with white to greyish hyphae, thick-walled, exuding creamy translucent conidial droplets from central ostioles. Conidiophores hyaline, smooth, septate, unbranched, densely aggregated, cylindrical, straight to sinuous, tapering towards apex, 10.0-14.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.3-2.3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. Conidiogenous cells 5.0-9.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.3-1.7
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, phialidic, cylindrical, terminal, slightly tapering towards apex. Alpha conidia hyaline, smooth, aseptate, biguttulate, ellipsoidal, oval, apex subobtuse, base subtruncate, 5.5-7.0
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-3.2
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 6.3
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<normalizedToken originalValue="×">x</normalizedToken>
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2.8
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Beta conidia hyaline, aseptate, filiform, mostly curved by 90-180°, tapering towards both ends, 26.0-36.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.6
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 21.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Gamma conidia not observed. Sexual morph not observed.
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</paragraph>
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<caption doi="10.3897/mycokeys.78.60878.figure6" httpUri="https://binary.pensoft.net/fig/511681" pageId="0" pageNumber="49" start="Figure 6" startId="F8">
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<paragraph pageId="0" pageNumber="49">
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<emphasis bold="true" pageId="0" pageNumber="49">Figure 6.</emphasis>
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<taxonomicName class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe middletonii" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="middletonii">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe middletonii</emphasis>
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</taxonomicName>
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(SAUCC194.46)
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<emphasis bold="true" pageId="0" pageNumber="49">a</emphasis>
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infected leaf of
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" genus="Lithocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Lithocarpus glaber" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="species" species="glaber">
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<emphasis italics="true" pageId="0" pageNumber="49">Lithocarpus glaber</emphasis>
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</taxonomicName>
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<emphasis bold="true" pageId="0" pageNumber="49">b, c</emphasis>
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surface and reverse of colony after 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="49">d, e</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="49">f-i</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="49">j</emphasis>
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beta conidia
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<emphasis bold="true" pageId="0" pageNumber="49">k, l</emphasis>
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alpha conidia and beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="49">f-l</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="description">
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<paragraph pageId="0" pageNumber="49">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="49">Cultures incubated on PDA at 25 °C in darkness, growth rate 22.5-24.0 mm diam/day, fluffy with abundant aerial mycelium, margin fimbriate, white on surface, white to pale yellow on reverse.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="materials_examined">
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<paragraph pageId="0" pageNumber="49">Specimens examined.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 19 April 2019, S.T. Huang. On diseased leaves of
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<taxonomicName class="Magnoliopsida" family="Sapindaceae" genus="Litchi" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Litchi chinensis" order="Sapindales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="species" species="chinensis">
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<emphasis italics="true" pageId="0" pageNumber="49">Litchi chinensis</emphasis>
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</taxonomicName>
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(
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<taxonomicName class="Magnoliopsida" family="Sapindaceae" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="" order="Sapindales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="family">Sapindaceae</taxonomicName>
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), HSAUP194.27, living culture SAUCC194.27; on diseased leaves of
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" genus="Lithocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Lithocarpus glaber" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="species" species="glaber">
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<emphasis italics="true" pageId="0" pageNumber="49">Lithocarpus glaber</emphasis>
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</taxonomicName>
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(
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="family">Fagaceae</taxonomicName>
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), HSAUP194.45, living culture SAUCC194.45; on diseased leaves of
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" genus="Lithocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Lithocarpus glaber" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="species" species="glaber">
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<emphasis italics="true" pageId="0" pageNumber="49">Lithocarpus glaber</emphasis>
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</taxonomicName>
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(
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="family">Fagaceae</taxonomicName>
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), 19 April 2019, S.T. Huang, HSAUP194.46, living culture SAUCC194.46; on diseased leaves of
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<taxonomicName authorityName="Barnett" authorityYear="1938" class="Dicotyledoneae" family="Fagaceae" genus="Lithocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Lithocarpus craibianus" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="species" species="craibianus">
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<emphasis italics="true" pageId="0" pageNumber="49">Lithocarpus craibianus</emphasis>
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</taxonomicName>
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(
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<taxonomicName class="Dicotyledoneae" family="Fagaceae" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="" order="Fagales" pageId="0" pageNumber="49" phylum="Angiospermae" rank="family">Fagaceae</taxonomicName>
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), HSAUP194.48, living culture SAUCC194.48.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="49" type="notes">
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<paragraph pageId="0" pageNumber="49">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="49">
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<taxonomicName class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe middletonii" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="middletonii">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe middletonii</emphasis>
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</taxonomicName>
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was originally described from the stem of
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<taxonomicName class="Insecta" family="Brassicaceae" genus="Rapistrum" higherTaxonomySource="GBIF" kingdom="Animalia" lsidName="Rapistrum rugosum" order="Brassicales" pageId="0" pageNumber="49" phylum="Arthropoda" rank="species" species="rugosum">
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<emphasis italics="true" pageId="0" pageNumber="49">Rapistrum rugosum</emphasis>
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</taxonomicName>
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(BRIP 54884e) (
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<taxonomicName class="Insecta" family="Brassicaceae" higherTaxonomySource="GBIF" kingdom="Animalia" lsidName="" order="Brassicales" pageId="0" pageNumber="49" phylum="Arthropoda" rank="family">Brassicaceae</taxonomicName>
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) and
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<taxonomicName class="Magnoliopsida" family="Asteraceae" genus="Chrysanthemoides" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Chrysanthemoides monilifera subsp. rotundata" order="Asterales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="subSpecies" species="monilifera" subSpecies="rotundata">Chrysanthemoides monilifera subsp. rotundata</taxonomicName>
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(BRIP 57329) (
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<taxonomicName class="Magnoliopsida" family="Asteraceae" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="family">Asteraceae</taxonomicName>
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) in Australia (
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<bibRefCitation DOI="https://doi.org/10.3767/003158515X687506" author="Thompson, SM" journalOrPublisher="Persoonia" pageId="0" pageNumber="49" pagination="39 - 49" refId="B51" refString="Thompson, SM, Tan, YP, Shivas, RG, Neate, SM, Morin, L, Bissett, A, Aitken, EAB, 2015. Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia 35 (1): 39 - 49, DOI: https://doi.org/10.3767/003158515X687506" title="Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia." url="https://doi.org/10.3767/003158515X687506" volume="35" year="2015">Thompson et al. 2015</bibRefCitation>
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). In the present study, four strains (SAUCC194.27, SAUCC194.45, SAUCC194.46 and SAUCC194.48) are closely related to
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<taxonomicName lsidName="D. middletonii" pageId="0" pageNumber="49" rank="species" species="middletonii">
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<emphasis italics="true" pageId="0" pageNumber="49">D. middletonii</emphasis>
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</taxonomicName>
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in the combined phylogenetic tree (Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe spp. based on combined sequence data of ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Codes referring to strains from the current study are written in red. Some branches were shortened to fit them to the page as indicated by two diagonal lines with the number of times a branch was shortened indicated." figureDoi="10.3897/mycokeys.78.60878.figure1" httpUri="https://binary.pensoft.net/fig/511674" pageId="0" pageNumber="49">1</figureCitation>
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). The differences between nucleotides in the concatenated alignment (17/565 in ITS, 9/494 in TUB and 10/340 in TEF) were minor. Morphologically, our strains were similar to
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<taxonomicName lsidName="D. middletonii" pageId="0" pageNumber="49" rank="species" species="middletonii">
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<emphasis italics="true" pageId="0" pageNumber="49">D. middletonii</emphasis>
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</taxonomicName>
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by slightly shorter and wider alpha conidia (5.0-7.0
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-3.2 vs. 6.0-7.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.0-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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), and longer beta conidia (26.0-36.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.6 vs. 20.0-35.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) (
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<bibRefCitation DOI="https://doi.org/10.3767/003158515X687506" author="Thompson, SM" journalOrPublisher="Persoonia" pageId="0" pageNumber="49" pagination="39 - 49" refId="B51" refString="Thompson, SM, Tan, YP, Shivas, RG, Neate, SM, Morin, L, Bissett, A, Aitken, EAB, 2015. Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia 35 (1): 39 - 49, DOI: https://doi.org/10.3767/003158515X687506" title="Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia." url="https://doi.org/10.3767/003158515X687506" volume="35" year="2015">Thompson et al. 2015</bibRefCitation>
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). We therefore identify our strains as
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<taxonomicName class="Sordariomycetes" family="Pandanaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe middletonii" order="Diaporthales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="middletonii">
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<emphasis italics="true" pageId="0" pageNumber="49">Diaporthe middletonii</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |