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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.55.34527" ID-GBIF-Dataset="7ec32d4f-0067-4c7f-88d0-b240d9fd6066" ID-PMC="PMC6614169" ID-Pensoft-Pub="1314-4049-55-101" ID-PubMed="31312097" ModsDocID="1314-4049-55-101" checkinTime="1565091262624" checkinUser="pensoft" docAuthor="Zeng, Zhao-Qing &amp; Zhuang, Wen-Ying" docDate="2019" docId="762FD0E23003550C9BD120A51AF95065" docLanguage="en" docName="MycoKeys 55: 101-120" docOrigin="MycoKeys 55" docSource="http://dx.doi.org/10.3897/mycokeys.55.34527" docTitle="Thelonectria guangdongensis Z. Q. Zeng &amp; W. Y. Zhuang, sp. nov." docType="treatment" docVersion="4" lastPageNumber="101" masterDocId="FF98FFE1D276D10F5338FF85FFAE5457" masterDocTitle="The genera Rugonectria and Thelonectria (Hypocreales, Nectriaceae) in China" masterLastPageNumber="120" masterPageNumber="101" pageNumber="101" updateTime="1668136261763" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>The genera Rugonectria and Thelonectria (Hypocreales, Nectriaceae) in China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zeng, Zhao-Qing</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhuang, Wen-Ying</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2019</mods:date>
<mods:detail type="volume">
<mods:number>55</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>101</mods:start>
<mods:end>120</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.55.34527</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.55.34527</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-55-101</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="159016271" LSID="urn:lsid:plazi:treatment:762FD0E23003550C9BD120A51AF95065" httpUri="http://treatment.plazi.org/id/762FD0E23003550C9BD120A51AF95065" lastPageNumber="101" pageId="0" pageNumber="101">
<subSubSection pageId="0" pageNumber="101" type="nomenclature">
<paragraph pageId="0" pageNumber="101">
<taxonomicName LSID="762fd0e2-3003-550c-9bd1-20a51af95065" authority="Z. Q. Zeng &amp; W. Y. Zhuang" class="Sordariomycetes" family="Nectriaceae" genus="Thelonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Thelonectria guangdongensis" order="Hypocreales" pageId="0" pageNumber="101" phylum="Ascomycota" rank="species" species="guangdongensis">Thelonectria guangdongensis Z.Q. Zeng &amp; W.Y. Zhuang</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="101">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Thelonectria guangdongensis a-d ascomata on natural substratum e colony on PDA f colony on SNA g median section through perithecium h-m ascospores n, q, r conidiogenous cells and macroconidia o, p, s-u macroconidia. Scale bars: 0.5 mm (a-d); 50 μm (g); 10 μm (h-u)." figureDoi="10.3897/mycokeys.55.34527.figure3" httpUri="https://binary.pensoft.net/fig/314521" pageId="0" pageNumber="101">Figure 3</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="holotype">
<paragraph pageId="0" pageNumber="101">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="101">
CHINA. Guangdong, Shixing, Chebaling, (
<geoCoordinate degrees="24" direction="north" minutes="43" orientation="latitude" precision="1" seconds="17.38" value="24.721495">24°43'17.38&quot;N</geoCoordinate>
,
<geoCoordinate degrees="114" direction="east" minutes="16" orientation="longitude" precision="1" seconds="39.50" value="114.27764">114°16'39.50&quot;E</geoCoordinate>
), alt. 600 m, on branches, 2 November 2015, Z.Q. Zeng, X.C. Wang, K. Chen, Y.B. Zhang 10627 (HMAS 254522); ex-type culture: HMAS 247233.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="sequences">
<paragraph pageId="0" pageNumber="101">Sequences.</paragraph>
<paragraph pageId="0" pageNumber="101">ACT (MF669045), ITS (MF669051), LSU (MF669053) and RPB1 (MF669057).</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="etymology">
<paragraph pageId="0" pageNumber="101">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="101">The specific epithet refers to the type locality of the fungus.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="description">
<paragraph pageId="0" pageNumber="101">Description.</paragraph>
<paragraph pageId="0" pageNumber="101">
Mycelium not visible around ascomata or on natural substrata. Ascomata perithecial, solitary to gregarious, up to 10 in a group, with a
<normalizedToken originalValue="welldeveloped">well-developed</normalizedToken>
stroma, superficial, subglobose to globose, bright red with a prominently darkened papilla, turning dark red in KOH, becoming slightly yellow in LA, 235-382
<normalizedToken originalValue="×">x</normalizedToken>
245-412
<normalizedToken originalValue="μm">μm</normalizedToken>
(n = 8). Perithecial surface slightly roughened. Perithecial wall of two layers, 20-50
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, outer layer 13-37
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, of textura intricata; inner layer 7.5-13
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, of textura prismatica. Asci not observed. Ascospores ellipsoid, 1-septate, smooth, 10-13
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="µm">µm</normalizedToken>
(11.6
<normalizedToken originalValue="±">+/-</normalizedToken>
1.3
<normalizedToken originalValue="×">x</normalizedToken>
4.2
<normalizedToken originalValue="±">+/-</normalizedToken>
0.7
<normalizedToken originalValue="μm">μm</normalizedToken>
). Colony on PDA 28 mm diameter after 7 d under daylight at 25 °C, surface velvety, with white aerial mycelium, producing purple pigment in medium. Colony on SNA 35 mm diameter after 7 d under daylight at 25 °C, surface with sparse whitish aerial mycelium. Phialides cylindrical or slightly swollen, 20-58
<normalizedToken originalValue="×">x</normalizedToken>
2-4
<normalizedToken originalValue="μm">μm</normalizedToken>
. Macroconidia cylindrical, slightly curved with rounded ends, 2-5-septate, 48-70
<normalizedToken originalValue="×">x</normalizedToken>
4.8-5.3
<normalizedToken originalValue="μm">μm</normalizedToken>
(58.9
<normalizedToken originalValue="±">+/-</normalizedToken>
7.14
<normalizedToken originalValue="×">x</normalizedToken>
5.0
<normalizedToken originalValue="±">+/-</normalizedToken>
0.2
<normalizedToken originalValue="μm">μm</normalizedToken>
). Microconidia and chlamydospores not observed in culture.
</paragraph>
<caption doi="10.3897/mycokeys.55.34527.figure3" httpUri="https://binary.pensoft.net/fig/314521" pageId="0" pageNumber="101" start="Figure 3" startId="F3">
<paragraph pageId="0" pageNumber="101">
Figure 3.
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Thelonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Thelonectria guangdongensis" order="Hypocreales" pageId="0" pageNumber="101" phylum="Ascomycota" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">Thelonectria guangdongensis</emphasis>
</taxonomicName>
<emphasis bold="true" pageId="0" pageNumber="101">
<normalizedToken originalValue="ad">a-d</normalizedToken>
</emphasis>
ascomata on natural substratum
<emphasis bold="true" pageId="0" pageNumber="101">e</emphasis>
colony on PDA
<emphasis bold="true" pageId="0" pageNumber="101">f</emphasis>
colony on SNA
<emphasis bold="true" pageId="0" pageNumber="101">g</emphasis>
median section through perithecium
<emphasis bold="true" pageId="0" pageNumber="101">
<normalizedToken originalValue="hm">h-m</normalizedToken>
</emphasis>
ascospores
<emphasis bold="true" pageId="0" pageNumber="101">n, q, r</emphasis>
conidiogenous cells and macroconidia
<emphasis bold="true" pageId="0" pageNumber="101">
o, p,
<normalizedToken originalValue="su">s-u</normalizedToken>
</emphasis>
macroconidia. Scale bars: 0.5 mm (
<emphasis bold="true" pageId="0" pageNumber="101">
<normalizedToken originalValue="ad">a-d</normalizedToken>
</emphasis>
); 50
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="101">g</emphasis>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="101">
<normalizedToken originalValue="hu">h-u</normalizedToken>
</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="habitat">
<paragraph pageId="0" pageNumber="101">Habitat.</paragraph>
<paragraph pageId="0" pageNumber="101">On branches.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="distribution">
<paragraph pageId="0" pageNumber="101">Distribution.</paragraph>
<paragraph pageId="0" pageNumber="101">Asia (China).</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="101" type="notes">
<paragraph pageId="0" pageNumber="101">Notes.</paragraph>
<paragraph pageId="0" pageNumber="101">
Amongst species of
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Thelonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Thelonectria" order="Hypocreales" pageId="0" pageNumber="101" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="101">Thelonectria</emphasis>
</taxonomicName>
,
<taxonomicName lsidName="T. guangdongensis" pageId="0" pageNumber="101" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. guangdongensis</emphasis>
</taxonomicName>
resembles
<taxonomicName lsidName="T. phoenicea" pageId="0" pageNumber="101" rank="species" species="phoenicea">
<emphasis italics="true" pageId="0" pageNumber="101">T. phoenicea</emphasis>
</taxonomicName>
in having subglobose to globose perithecia with slightly roughened surface, purple colony, lack of microconidia and number of septa in macroconidia (
<bibRefCitation DOI="https://doi.org/10.1007/s13225-014-0280-y" author="Salgado-Salazar, C" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="101" pagination="1 - 29" refId="B26" refString="Salgado-Salazar, C, Rossman, AY, Samuels, GJ, Hirooka, Y, Sanchez, RM, Chaverri, P, 2015. Phylogeny and taxonomic revision of Thelonectriadiscophora (Ascomycota, Hypocreales, Nectriaceae) species complex. . Fungal Diversity 70: 1 - 29" title="Phylogeny and taxonomic revision of Thelonectriadiscophora (Ascomycota, Hypocreales, Nectriaceae) species complex." url="https://doi.org/10.1007/s13225-014-0280-y" volume="70" year="2015">Salgado-Salazar et al. 2015</bibRefCitation>
). However,
<taxonomicName lsidName="T. phoenicea" pageId="0" pageNumber="101" rank="species" species="phoenicea">
<emphasis italics="true" pageId="0" pageNumber="101">T. phoenicea</emphasis>
</taxonomicName>
has much larger perithecia 300-600
<normalizedToken originalValue="×">x</normalizedToken>
200-350
<normalizedToken originalValue="μm">μm</normalizedToken>
, wider ascospores that are 4-5.5
<normalizedToken originalValue="μm">μm</normalizedToken>
wide, and wider phialides 3-6.5
<normalizedToken originalValue="μm">μm</normalizedToken>
wide (
<bibRefCitation DOI="https://doi.org/10.1007/s13225-014-0280-y" author="Salgado-Salazar, C" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="101" pagination="1 - 29" refId="B26" refString="Salgado-Salazar, C, Rossman, AY, Samuels, GJ, Hirooka, Y, Sanchez, RM, Chaverri, P, 2015. Phylogeny and taxonomic revision of Thelonectriadiscophora (Ascomycota, Hypocreales, Nectriaceae) species complex. . Fungal Diversity 70: 1 - 29" title="Phylogeny and taxonomic revision of Thelonectriadiscophora (Ascomycota, Hypocreales, Nectriaceae) species complex." url="https://doi.org/10.1007/s13225-014-0280-y" volume="70" year="2015">Salgado-Salazar et al. 2015</bibRefCitation>
). Moreover, there are 13 bp, 44 bp, 8 bp and 54 bp divergences in the ACT, ITS, LSU and RPB1 regions, respectively, between the type of
<taxonomicName lsidName="T. guangdongensis" pageId="0" pageNumber="101" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. guangdongensis</emphasis>
</taxonomicName>
(HMAS 254522) and that of
<taxonomicName lsidName="T. phoenicea" pageId="0" pageNumber="101" rank="species" species="phoenicea">
<emphasis italics="true" pageId="0" pageNumber="101">T. phoenicea</emphasis>
</taxonomicName>
(G.J.S. 85-179).
</paragraph>
<paragraph pageId="0" pageNumber="101">
Phylogenetically
<taxonomicName lsidName="T. guangdongensis" pageId="0" pageNumber="101" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. guangdongensis</emphasis>
</taxonomicName>
is closely related to
<taxonomicName lsidName="T. beijingensis" pageId="0" pageNumber="101" rank="species" species="beijingensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. beijingensis</emphasis>
</taxonomicName>
with strong statistical support (MLBP/MPBP/BIPP = 100%/97%/100%) (
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A Maximum Parsimony tree inferred from the combined ACT, ITS, LSU and RPB 1 sequences. Cosmospora coccinea and Nectria cinnabarina were used as outgroup taxa. MLBP (left) and MPBP (middle) above 50 %, BIPP (right) above 90 % are indicated at nodes." figureDoi="10.3897/mycokeys.55.34527.figure1" httpUri="https://binary.pensoft.net/fig/314519" pageId="0" pageNumber="101">Figure 1</figureCitation>
). However,
<taxonomicName lsidName="T. beijingensis" pageId="0" pageNumber="101" rank="species" species="beijingensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. beijingensis</emphasis>
</taxonomicName>
differs in having larger ascospores that are 13-17
<normalizedToken originalValue="×">x</normalizedToken>
4-7
<normalizedToken originalValue="μm">μm</normalizedToken>
, while those of
<taxonomicName lsidName="T. guangdongensis" pageId="0" pageNumber="101" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. guangdongensis</emphasis>
</taxonomicName>
are 10-13
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="µm">µm</normalizedToken>
and form microconidia in culture in addition to macroconidia (
<bibRefCitation DOI="https://doi.org/10.11646/phytotaxa.85.1.3" author="Zeng, ZQ" journalOrPublisher="Phytotaxa" pageId="0" pageNumber="101" pagination="15 - 25" refId="B37" refString="Zeng, ZQ, Zhuang, WY, 2013. Four new taxa of Ilyonectria and Thelonectria (Nectriaceae) revealed by morphology and combined ITS and β-tubulin sequence data. . Phytotaxa 85: 15 - 25" title="Four new taxa of Ilyonectria and Thelonectria (Nectriaceae) revealed by morphology and combined ITS and β-tubulin sequence data." url="https://doi.org/10.11646/phytotaxa.85.1.3" volume="85" year="2013">Zeng and Zhuang 2013</bibRefCitation>
). There are 20 bp, 30 bp, 5 bp and 50 bp divergences in the ACT, ITS, LSU and RPB1 regions between the ex-type culture of
<taxonomicName lsidName="T. guangdongensis" pageId="0" pageNumber="101" rank="species" species="guangdongensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. guangdongensis</emphasis>
</taxonomicName>
and that of
<taxonomicName lsidName="T. beijingensis" pageId="0" pageNumber="101" rank="species" species="beijingensis">
<emphasis italics="true" pageId="0" pageNumber="101">T. beijingensis</emphasis>
</taxonomicName>
(HMAS 188498). Both morphology and molecular data support the establishment of the new species.
</paragraph>
</subSubSection>
</treatment>
</document>