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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.67.52055" ID-GBIF-Dataset="ad20a203-0984-4ab6-bdb8-71b078dba7af" ID-PMC="PMC7270078" ID-Pensoft-Pub="1314-4049-67-55" ID-Pensoft-UUID="CE9642BF68865BC2B7929C4F7076DCAE" ID-PubMed="32547306" ModsDocID="1314-4049-67-55" checkinTime="1590646543563" checkinUser="pensoft" docAuthor="Valle, Laia Guardia &amp; Stoianova, Desislava" docDate="2020" docId="78D22DC9A2A85535A18740E3A3D5CD0E" docLanguage="en" docName="MycoKeys 67: 55-80" docOrigin="MycoKeys 67" docSource="http://dx.doi.org/10.3897/mycokeys.67.52055" docTitle="Graminella bulbosa Leger &amp; Gauthier, 1937 ex Manier 1962" docType="treatment" docVersion="4" id="CE9642BF68865BC2B7929C4F7076DCAE" lastPageNumber="55" masterDocId="CE9642BF68865BC2B7929C4F7076DCAE" masterDocTitle="First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia" masterLastPageNumber="80" masterPageNumber="55" pageNumber="55" updateTime="1668136459135" updateUser="ExternalLinkService">
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<mods:title>First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Valle, Laia Guardia</mods:namePart>
<mods:affiliation>Unitat de Botanica, Dept. Biologia Animal, Biologia Vegetal i d'Ecologia. Fac. Biociences. Universitat Autonoma de Barcelona. 08193 - Bellaterra (Barcelona), Spain</mods:affiliation>
<mods:nameIdentifier type="orcid">https://orcid.org/0000-0001-8295-1093</mods:nameIdentifier>
<mods:nameIdentifier type="email">laia.guardia@uab.cat</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Stoianova, Desislava</mods:namePart>
<mods:affiliation>Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences. Sofia, Bulgaria</mods:affiliation>
<mods:nameIdentifier type="orcid">https://orcid.org/0000-0001-8040-7158</mods:nameIdentifier>
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<mods:title>MycoKeys</mods:title>
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<mods:part>
<mods:date>2020</mods:date>
<mods:detail type="volume">
<mods:number>67</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>55</mods:start>
<mods:end>80</mods:end>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.67.52055</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.67.52055</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-67-55</mods:identifier>
<mods:identifier type="Pensoft-UUID">CE9642BF68865BC2B7929C4F7076DCAE</mods:identifier>
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<treatment ID-GBIF-Taxon="164363115" LSID="urn:lsid:plazi:treatment:78D22DC9A2A85535A18740E3A3D5CD0E" httpUri="http://treatment.plazi.org/id/78D22DC9A2A85535A18740E3A3D5CD0E" lastPageNumber="55" pageId="0" pageNumber="55">
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<paragraph pageId="0" pageNumber="55">
<taxonomicName LSID="78D22DC9-A2A8-5535-A187-40E3A3D5CD0E" authority="Leger &amp; Gauthier, 1937 ex Manier, 1962." authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
Graminella bulbosa
<normalizedToken originalValue="Léger">Leger</normalizedToken>
&amp; Gauthier, 1937 ex Manier, 1962.
</taxonomicName>
<figureCitation captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">Figs 11-13</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="55" type="materials_examined">
<paragraph pageId="0" pageNumber="55">Specimens examined.</paragraph>
<paragraph pageId="0" pageNumber="55">Site 2: slide BUL-2-5; Site 8: slides BUL-8-1, BUL-8-2.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="55" type="notes">
<paragraph pageId="0" pageNumber="55">Notes.</paragraph>
<paragraph pageId="0" pageNumber="55">
This species is characterised by the unusual formation of vegetative propagules from the bulbous basal cells (Fig.
<figureCitation captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">12</figureCitation>
), a feature only shared with the related genus
<taxonomicName authorityName="R.W.Lichtwardt" authorityYear="1983" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Gauthieromyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Gauthieromyces" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="55">Gauthieromyces</emphasis>
</taxonomicName>
(
<bibRefCitation author="Lichtwardt, RW" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="55" pagination="213 - 215" refId="B21" refString="Lichtwardt, RW, 1983. Gauthieromyces, a new genus of Harpellales based on Genistella microspora. Mycotaxon 17: 213 - 215" title="Gauthieromyces, a new genus of Harpellales based on Genistella microspora." volume="17" year="1983">Lichtwardt 1983</bibRefCitation>
).
<taxonomicName authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
was described from France (
<bibRefCitation author="Leger, L" journalOrPublisher="Comptes Rendus de l'Academie des Sciences, Paris" pageId="0" pageNumber="55" pagination="27 - 29" refId="B19" refString="Leger, L, Gauthier, M, 1937. Graminella bulbosa nouveau genre d'Entophyte parasite des larves d'ephemerides du genre Baetis. Comptes Rendus de l'Academie des Sciences, Paris 202: 27 - 29" title="Graminella bulbosa nouveau genre d'Entophyte parasite des larves d'ephemerides du genre Baetis." volume="202" year="1937">
<normalizedToken originalValue="Léger">Leger</normalizedToken>
and Gauthier 1937
</bibRefCitation>
; Manier 1962). The species is also known from Spain (
<bibRefCitation DOI="https://doi.org/10.1080/15572536.2007.11832569" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="442 - 455" refId="B58" refString="Valle, LG, 2007. New species and summary of Iberian Harpellales. Mycologia 99: 442 - 455, DOI: https://doi.org/10.1080/15572536.2007.11832569" title="New species and summary of Iberian Harpellales." url="https://doi.org/10.1080/15572536.2007.11832569" volume="99" year="2007">Valle 2007</bibRefCitation>
), Portugal (
<bibRefCitation DOI="https://doi.org/10.3852/12-211" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="748 - 759" refId="B59" refString="Valle, LG, 2013a. New and rare Harpellales from Portugal and northwestern Iberian Peninsula: discovering the hidden mycobiota of Galicia-Tras-os-Montes region. Mycologia 105: 748 - 759, DOI: https://doi.org/10.3852/12-211" title="New and rare Harpellales from Portugal and northwestern Iberian Peninsula: discovering the hidden mycobiota of Galicia-Tras-os-Montes region." url="https://doi.org/10.3852/12-211" volume="105" year="2013 a">Valle 2013a</bibRefCitation>
) and Italy (
<bibRefCitation DOI="https://doi.org/10.3852/12-184" author="Valle, LG" journalOrPublisher="Mycologia" pageId="0" pageNumber="55" pagination="712 - 727" refId="B67" refString="Valle, LG, Rossi, W, Santamaria, S, 2013. New species and new records of trichomycetes from Italy. Mycologia 105: 712 - 727, DOI: https://doi.org/10.3852/12-184" title="New species and new records of trichomycetes from Italy." url="https://doi.org/10.3852/12-184" volume="105" year="2013">Valle et al. 2013</bibRefCitation>
).
<taxonomicName authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
has been reported associated with various species of
<taxonomicName authorityName="Leach" authorityYear="1815" class="Insecta" family="Baetidae" genus="Baetis" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Baetis" order="Ephemeroptera" pageId="0" pageNumber="55" phylum="Arthropoda" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="55">Baetis</emphasis>
</taxonomicName>
and related genera, very frequently within the hindgut of
<taxonomicName lsidName="B. rhodani" pageId="0" pageNumber="55" rank="species" species="rhodani">
<emphasis italics="true" pageId="0" pageNumber="55">B. rhodani</emphasis>
</taxonomicName>
(Pictet). This species of mayfly is common and widespread in Europe and it also hosted Bulgarian specimens of
<taxonomicName lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
in the surveyed rivers, together with
<taxonomicName lsidName="B. alpinus" pageId="0" pageNumber="55" rank="species" species="alpinus">
<emphasis italics="true" pageId="0" pageNumber="55">B. alpinus</emphasis>
</taxonomicName>
(Pictet). In fact, the genus
<taxonomicName authorityName="Leach" authorityYear="1815" class="Insecta" family="Baetidae" genus="Baetis" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Baetis" order="Ephemeroptera" pageId="0" pageNumber="55" phylum="Arthropoda" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="55">Baetis</emphasis>
</taxonomicName>
bears different Harpellid species, including the more common
<taxonomicName authorityName="Pouzar" authorityYear="1972" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Legeriomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Legeriomyces ramosus" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="ramosus">
<emphasis italics="true" pageId="0" pageNumber="55">Legeriomyces ramosus</emphasis>
</taxonomicName>
, occasionally sharing the same gut lumen with
<taxonomicName authorityName="Leger &amp; Gauthier, 1937 ex Manier" authorityYear="1962" class="Kickxellomycetes" family="Legeriomycetaceae" genus="Graminella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Graminella bulbosa" order="Harpellales" pageId="0" pageNumber="55" phylum="Zygomycota" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">Graminella bulbosa</emphasis>
</taxonomicName>
. Bulgarian specimens of
<taxonomicName lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
show the typical small and numerous trichospores (Fig.
<figureCitation captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">13</figureCitation>
), measuring 8 -11
<normalizedToken originalValue="×">x</normalizedToken>
2
<normalizedToken originalValue="µm">µm</normalizedToken>
in our collections. These measurements are midway between
<taxonomicName lsidName="G. bulbosa" pageId="0" pageNumber="55" rank="species" species="bulbosa">
<emphasis italics="true" pageId="0" pageNumber="55">G. bulbosa</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="G. microsporus" pageId="0" pageNumber="55" rank="species" species="microsporus">
<emphasis italics="true" pageId="0" pageNumber="55">G. microsporus</emphasis>
</taxonomicName>
(see discussion for further information). Unfortunately, only immature zygospores were observed (Fig.
<figureCitation captionStart="Figures 1116" captionStartId="F3" captionText="Figures 11 - 16. Various species of Harpellales. 11 - 13 Graminella microspora from Baetidae nymphs: 11 thallus overview, with inflated basal cell structures from which propagules arise or are extruded (note: one empty basal cell which has extruded its cellular content, arrow); young zygospores can also be observed (arrowhead) 12 a cluster of basal cells 13 fertile branch with a long series of minute trichospores; 14, 15 Harpella melusinae from Simuliidae larvae: 14 generative cells and allantoids or curved trichospores 15 basal cell and small conic holdfast 16 Legeriomyces ramosus from Baetidae nymphs, zygospores and zygosporophores. Scale bars: 25 μm in all figures." figureDoi="10.3897/mycokeys.67.52055.figures11-16" httpUri="https://binary.pensoft.net/fig/414541" pageId="0" pageNumber="55">11</figureCitation>
arrowhead).
</paragraph>
</subSubSection>
</treatment>
</document>