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<document ID-DOI="10.11646/phytotaxa.587.3.3" ID-ISSN="1179-3163" ID-Zenodo-Dep="7744311" checkinTime="1679049297477" checkinUser="plazi" docAuthor="Li, Hua, Manawasinghe, Ishara S., Zhang, Yunxia &amp; Senanayake, Indunil C." docDate="2023" docId="6732879F9F6BFFAEFF042D9B19CED7F7" docLanguage="en" docName="phytotaxa.587.3.3.pdf" docOrigin="Phytotaxa 587 (3)" docSource="http://dx.doi.org/10.11646/phytotaxa.587.3.3" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.5:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="5" docTitle="Pestalotiopsis linguae Li Hua, Manawas., &amp; YX Zhang. 2023, sp. nov." docType="treatment" docVersion="1" lastPageNumber="242" masterDocId="9B0BFFE79F67FFA3FF8C2B33192FD427" masterDocTitle="Taxonomic and phylogenic appraisal of Pestalotiopsis linguae sp. nov., and a new record of P. nanjingensis from Pyrrosia lingua (Polypodiaceae) in Southern China" masterLastPageNumber="250" masterPageNumber="229" pageNumber="241" updateTime="1679322057807" updateUser="jonas" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:titleInfo>
<mods:title>Taxonomic and phylogenic appraisal of Pestalotiopsis linguae sp. nov., and a new record of P. nanjingensis from Pyrrosia lingua (Polypodiaceae) in Southern China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Li, Hua</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0002-7832-4289</mods:nameIdentifier>
<mods:affiliation>Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, China &amp; freda _ lh @ 163. com; https: // orcid. org / 0000 - 0002 - 7832 - 4289</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Manawasinghe, Ishara S.</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0001-5730-3596</mods:nameIdentifier>
<mods:affiliation>Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China &amp; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, China &amp; ishara. ishara @ yahoo. com; https: // orcid. org / 0000 - 0001 - 5730 - 3596</mods:affiliation>
<mods:nameIdentifier type="email">ishara.ishara@yahoo.com</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhang, Yunxia</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0002-2815-2554</mods:nameIdentifier>
<mods:affiliation>Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China &amp; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, China &amp; yx _ zhang 08 @ 163. com; https: // orcid. org / 0000 - 0002 - 2815 - 2554</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Senanayake, Indunil C.</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0002-7165-2394</mods:nameIdentifier>
<mods:affiliation>Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China &amp; Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510225, China &amp; indunilchinthani @ gmail. com; https: // orcid. org / 0000 - 0002 - 7165 - 2394</mods:affiliation>
<mods:nameIdentifier type="email">indunilchinthani@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Phytotaxa</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2023</mods:date>
<mods:detail type="pubDate">
<mods:number>2023-03-16</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>587</mods:number>
</mods:detail>
<mods:detail type="issue">
<mods:number>3</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>229</mods:start>
<mods:end>250</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.11646/phytotaxa.587.3.3</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">10.11646/phytotaxa.587.3.3</mods:identifier>
<mods:identifier type="ISSN">1179-3163</mods:identifier>
<mods:identifier type="Zenodo-Dep">7744311</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:6732879F9F6BFFAEFF042D9B19CED7F7" httpUri="http://treatment.plazi.org/id/6732879F9F6BFFAEFF042D9B19CED7F7" lastPageId="13" lastPageNumber="242" pageId="12" pageNumber="241">
<subSubSection box="[136,1026,1704,1731]" pageId="12" pageNumber="241" type="nomenclature">
<paragraph blockId="12.[136,1026,1704,1731]" box="[136,1026,1704,1731]" pageId="12" pageNumber="241">
<heading box="[136,1026,1704,1731]" fontSize="11" level="2" pageId="12" pageNumber="241" reason="3">
<taxonomicName authority="Li Hua, Manawas., &amp; YX Zhang." authorityName="Li Hua, Manawas., &amp; YX Zhang." authorityYear="2023" box="[136,769,1704,1731]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="12" pageNumber="241" phylum="Ascomycota" rank="species" species="linguae" status="sp. nov.">
<emphasis bold="true" box="[136,387,1704,1731]" italics="true" pageId="12" pageNumber="241">Pestalotiopsis linguae</emphasis>
Li Hua, Manawas., &amp; YX Zhang.
</taxonomicName>
<emphasis bold="true" box="[776,1020,1704,1730]" pageId="12" pageNumber="241">
<taxonomicNameLabel box="[776,864,1704,1730]" pageId="12" pageNumber="241" rank="species">
<emphasis bold="true" box="[776,857,1704,1730]" italics="true" pageId="12" pageNumber="241">sp. nov</emphasis>
.
</taxonomicNameLabel>
(
<figureCitation box="[878,1011,1704,1730]" captionStart="FIGURE 3" captionStartId="13.[136,229,1929,1951]" captionTargetBox="[151,1436,1034,1902]" captionTargetId="figure-408@13.[151,1436,1034,1902]" captionTargetPageId="13" captionText="FIGURE 3. Pestalotiopsis linguae (ZHKU 22-0089, Holotype) a. Colony on PDA after 5 days at 25℃—surface view and reverse. b. Conidioma formed on PDA. c-d. Conidiophores, conidiogenous cells and attached conidia. e-g. Conidia. Scale bars: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.7744321" httpUri="https://zenodo.org/record/7744321/files/figure.png" pageId="12" pageNumber="241">FIGURE 3</figureCitation>
)
</emphasis>
.
</heading>
</paragraph>
</subSubSection>
<subSubSection pageId="12" pageNumber="241" type="multiple">
<paragraph blockId="12.[136,915,1744,1874]" box="[136,507,1744,1766]" pageId="12" pageNumber="241">Index Fungourum number: IF 559839;</paragraph>
<paragraph blockId="12.[136,915,1744,1874]" box="[136,470,1780,1802]" pageId="12" pageNumber="241">Facesoffungi number: FOF 12712.</paragraph>
</subSubSection>
<subSubSection box="[136,915,1816,1838]" pageId="12" pageNumber="241" type="etymology">
<paragraph blockId="12.[136,915,1744,1874]" box="[136,915,1816,1838]" pageId="12" pageNumber="241">Etymology: Epithet refers to the host species from which the fungus was isolated</paragraph>
</subSubSection>
<subSubSection box="[136,390,1852,1874]" pageId="12" pageNumber="241" type="materials_examined">
<paragraph blockId="12.[136,915,1744,1874]" box="[136,390,1852,1874]" pageId="12" pageNumber="241">
<materialsCitation box="[136,390,1852,1874]" collectionCode="ZHKU" pageId="12" pageNumber="241" specimenCode="ZHKU 22-0089" specimenCount="1" typeStatus="holotype">
<typeStatus box="[136,229,1852,1874]" pageId="12" pageNumber="241">Holotype</typeStatus>
:
<specimenCode box="[238,390,1852,1873]" collectionCode="ZHKU" pageId="12" pageNumber="241">ZHKU 22-0089</specimenCode>
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection lastPageId="13" lastPageNumber="242" pageId="12" pageNumber="241" type="description">
<paragraph blockId="12.[136,1452,1920,2091]" lastBlockId="13.[136,1452,158,976]" lastPageId="13" lastPageNumber="242" pageId="12" pageNumber="241">
<emphasis box="[136,264,1920,1946]" italics="true" pageId="12" pageNumber="241">Endophytic</emphasis>
on the
<taxonomicName box="[341,514,1920,1946]" class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="12" pageNumber="241" phylum="Tracheophyta" rank="species" species="lingua">
<emphasis box="[341,514,1920,1946]" italics="true" pageId="12" pageNumber="241">Pyrrosia lingua</emphasis>
</taxonomicName>
(
<taxonomicName box="[528,694,1920,1946]" class="Polypodiopsida" family="Polypodiaceae" kingdom="Plantae" order="Polypodiales" pageId="12" pageNumber="241" phylum="Tracheophyta" rank="family">
<emphasis box="[528,694,1920,1946]" italics="true" pageId="12" pageNumber="241">Polypodiaceae</emphasis>
</taxonomicName>
) leaves.
<emphasis bold="true" box="[791,951,1920,1947]" italics="true" pageId="12" pageNumber="241">Sexual morph</emphasis>
: not observed.
<emphasis bold="true" box="[1118,1292,1920,1947]" italics="true" pageId="12" pageNumber="241">Asexual morph</emphasis>
:
<emphasis italics="true" pageId="12" pageNumber="241">Conidiomata acervular</emphasis>
, 8001,100 μm diameter, globose, solitary or aggregated in clusters, exuding black conidial masses.
<emphasis box="[136,301,1992,2018]" italics="true" pageId="12" pageNumber="241">Conidiophores</emphasis>
reduced to conidiogenous cells.
<emphasis box="[659,884,1992,2018]" italics="true" pageId="12" pageNumber="241">Conidiogenous cells</emphasis>
discrete or integrated, cylindrical to subcylindrical, hyaline, smooth, annelidic.
<emphasis box="[456,546,2028,2054]" italics="true" pageId="12" pageNumber="241">Conidia</emphasis>
(16.5)1821(23) × (4)57(8) μm (x̅±SD=19.2±1.4 × 5.9±0.8 μm, n=30), fusoid, ellipsoid, straight or slightly curved, 4-septate; basal cell conical, (2)35 μm, hyaline, smooth, thin-walled; with a single appendage (3)47(8) μm (x̅±SD=5±1.3 μm, n=30) long, filiform, unbranched, centric; three median cellsdoliiform, (11)11.513(14) μm (x̅±SD=12.2±0.7 μm, n=30) long, smooth, concolorous, brown, septa darker than the restof the cell (second cell from base (3)45 μm long; third cell (2)34(5) μm long; fourth cell (3)45 μm long); apical cell conical, (2.5)34(4.5) μm (x̅±SD=3.7±0.5 μm, n=30) long, hyaline, smooth, thin-walled; with 23 apicaltubular appendages (10)1217(21.5) μm (x̅±SD=14.9±2.5 μm, n=30) long, unbranched, filiform.
</paragraph>
</subSubSection>
<subSubSection pageId="13" pageNumber="242" type="biology_ecology">
<paragraph blockId="13.[136,1452,158,976]" pageId="13" pageNumber="242">
<emphasis box="[189,446,338,364]" italics="true" pageId="13" pageNumber="242">Culture characteristics</emphasis>
: Colonies on PDA reaching
<quantity box="[762,839,338,365]" metricMagnitude="-2" metricUnit="m" metricValue="5.0" pageId="13" pageNumber="242" unit="mm" value="50.0">50 mm</quantity>
diameter after five days at 25 ℃. Colonies filamentous to circular, medium dense, white aerial mycelium on surface flat or raised.
</paragraph>
</subSubSection>
<subSubSection pageId="13" pageNumber="242" type="materials_examined">
<paragraph blockId="13.[136,1452,158,976]" pageId="13" pageNumber="242">
<emphasis box="[189,403,410,436]" italics="true" pageId="13" pageNumber="242">Material examined</emphasis>
:
<materialsCitation collectionCode="ZHKU, ZHKUCC" country="China" latitude="23.186943" location="Li Hua" longLatPrecision="21" longitude="113.2975" municipality="Guangzhou City" pageId="13" pageNumber="242" specimenCode="ZHKU 22-0089, ZHKUCC 22-0159" specimenCount="1" stateProvince="Guangdong" typeStatus="holotype">
<collectingCountry box="[420,512,410,436]" name="China" pageId="13" pageNumber="242">CHINA</collectingCountry>
,
<collectingRegion box="[524,765,410,436]" country="China" name="Guangdong" pageId="13" pageNumber="242">Guangdong Province</collectingRegion>
,
<collectingMunicipality box="[778,965,410,436]" pageId="13" pageNumber="242">Guangzhou City</collectingMunicipality>
, from the leaf of
<taxonomicName authority="(Thunb.) Farwell" authorityName="Farwell" baseAuthorityName="Thunb." class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="lingua">
<emphasis box="[1167,1344,410,436]" italics="true" pageId="13" pageNumber="242">Pyrrosia lingua</emphasis>
(Thunb.) Farwell
</taxonomicName>
(
<geoCoordinate box="[236,373,446,472]" degrees="23" direction="north" minutes="11" orientation="latitude" pageId="13" pageNumber="242" precision="15" seconds="13" value="23.186943">23°1113”N</geoCoordinate>
<geoCoordinate box="[379,529,446,473]" degrees="113" direction="east" minutes="17" orientation="longitude" pageId="13" pageNumber="242" precision="15" seconds="51" value="113.2975">113°1751”E</geoCoordinate>
), 16 August. 2021,
<location LSID="urn:lsid:plazi:treatment:6732879F9F6BFFAEFF042D9B19CED7F7:EA4460529F6AFFAEFD6B2A8D1A1AD5FE" box="[743,821,446,473]" country="China" latitude="23.186943" longLatPrecision="21" longitude="113.2975" municipality="Guangzhou City" name="Li Hua" pageId="13" pageNumber="242" stateProvince="Guangdong">Li Hua</location>
(
<typeStatus box="[834,934,446,472]" pageId="13" pageNumber="242">holotype</typeStatus>
<specimenCode box="[940,1117,446,473]" collectionCode="ZHKU" pageId="13" pageNumber="242">ZHKU 22-0089</specimenCode>
as dry culture); ex-type living culture
<specimenCode box="[221,439,482,509]" collectionCode="ZHKUCC" pageId="13" pageNumber="242">ZHKUCC 22-0159</specimenCode>
</materialsCitation>
,
<materialsCitation box="[450,720,482,508]" collectionCode="ZHKUCC" country="China" latitude="23.186943" location="Li Hua" longLatPrecision="21" longitude="113.2975" municipality="Guangzhou City" pageId="13" pageNumber="242" specimenCode="ZHKUCC 22-0160" specimenCount="1" stateProvince="Guangdong Province" typeStatus="holotype">
<emphasis box="[450,494,482,508]" italics="true" pageId="13" pageNumber="242">ibid</emphasis>
<specimenCode box="[501,720,482,508]" collectionCode="ZHKUCC" pageId="13" pageNumber="242">ZHKUCC 22-0160</specimenCode>
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="13" pageNumber="242" type="discussion">
<paragraph blockId="13.[136,1452,158,976]" pageId="13" pageNumber="242">
<emphasis box="[189,253,519,544]" italics="true" pageId="13" pageNumber="242">Notes</emphasis>
: Two isolates in this study developed a clustered with 78% ML and 1.00 BYPP values. In the phylogenetic tree, our isolates show a particular neighbouring to seven known
<emphasis box="[868,1011,554,580]" italics="true" pageId="13" pageNumber="242">Pestalotipsis</emphasis>
species, but they differ in morphology. The length of the base appendage of
<taxonomicName box="[543,654,590,616]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="13" pageNumber="242" phylum="Ascomycota" rank="species" species="linguae">
<emphasis box="[543,654,590,616]" italics="true" pageId="13" pageNumber="242">P. linguae</emphasis>
</taxonomicName>
is longer than that of
<taxonomicName box="[895,1042,590,616]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="formosana">
<emphasis box="[895,1042,590,616]" italics="true" pageId="13" pageNumber="242">P. formosana</emphasis>
</taxonomicName>
(47 μm vs. 35 μm),
<taxonomicName box="[1294,1452,590,616]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="kandelicola">
<emphasis box="[1294,1452,590,616]" italics="true" pageId="13" pageNumber="242">P. kandelicola</emphasis>
</taxonomicName>
(47 μm vs. 2.53 μm),
<taxonomicName box="[407,499,627,652]" class="Magnoliopsida" family="Ericaceae" genus="Pieris" kingdom="Plantae" order="Ericales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="parva">
<emphasis box="[407,499,627,652]" italics="true" pageId="13" pageNumber="242">P. parva</emphasis>
</taxonomicName>
(47 μm vs. 24 μm), and
<taxonomicName box="[803,934,627,652]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="etonensis">
<emphasis box="[803,934,627,652]" italics="true" pageId="13" pageNumber="242">P. etonensis</emphasis>
</taxonomicName>
(47 μm vs. 24.5 μm). The length of conidia of
<taxonomicName box="[166,277,662,688]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="13" pageNumber="242" phylum="Ascomycota" rank="species" species="linguae">
<emphasis box="[166,277,662,688]" italics="true" pageId="13" pageNumber="242">P. linguae</emphasis>
</taxonomicName>
is shorter than that of
<taxonomicName box="[525,682,662,688]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="kandelicola">
<emphasis box="[525,682,662,688]" italics="true" pageId="13" pageNumber="242">P. kandelicola</emphasis>
</taxonomicName>
(1821 μm vs. 2023.5 μm),
<taxonomicName box="[1012,1187,663,688]" class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="nanningensis">
<emphasis box="[1012,1187,663,688]" italics="true" pageId="13" pageNumber="242">P. nanningensis</emphasis>
</taxonomicName>
(1821 μm vs. 2426.5 μm),
<taxonomicName box="[197,354,698,724]" class="Magnoliopsida" family="Proteaceae" genus="Protea" kingdom="Plantae" order="Proteales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="thailandica">
<emphasis box="[197,354,698,724]" italics="true" pageId="13" pageNumber="242">P. thailandica</emphasis>
</taxonomicName>
(1821 μm vs. 2125 μm),
<taxonomicName box="[680,844,698,724]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="rhizophorae">
<emphasis box="[680,844,698,724]" italics="true" pageId="13" pageNumber="242">P. rhizophorae</emphasis>
</taxonomicName>
(1821 μm vs. 20.527 μm), but its conidia is longer than
<taxonomicName box="[191,323,735,760]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="etonensis">
<emphasis box="[191,323,735,760]" italics="true" pageId="13" pageNumber="242">P. etonensis</emphasis>
</taxonomicName>
(1821 μm vs. 1521 μm). The apical appendage of
<taxonomicName box="[918,1030,734,760]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="13" pageNumber="242" phylum="Ascomycota" rank="species" species="linguae">
<emphasis box="[918,1030,734,760]" italics="true" pageId="13" pageNumber="242">P. linguae</emphasis>
</taxonomicName>
is longer than that of
<taxonomicName box="[1276,1452,735,760]" class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="nanningensis">
<emphasis box="[1276,1452,735,760]" italics="true" pageId="13" pageNumber="242">P. nanningensis</emphasis>
</taxonomicName>
(1217 μm). vs. 1822.5 μm) shorter and longer than
<taxonomicName box="[747,840,771,796]" class="Magnoliopsida" family="Ericaceae" genus="Pieris" kingdom="Plantae" order="Ericales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="parva">
<emphasis box="[747,840,771,796]" italics="true" pageId="13" pageNumber="242">P. parva</emphasis>
</taxonomicName>
(1217 μm vs. 6.512 μm). In addition,
<taxonomicName box="[1305,1405,770,796]" class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="lingua">
<emphasis box="[1305,1405,770,796]" italics="true" pageId="13" pageNumber="242">P. lingua</emphasis>
</taxonomicName>
can also differ by
<taxonomicName box="[297,446,806,832]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="formosana">
<emphasis box="[297,446,806,832]" italics="true" pageId="13" pageNumber="242">P. formosana</emphasis>
</taxonomicName>
based on the base pair differences (2/
<quantity box="[880,954,806,833]" metricMagnitude="1" metricUnit="m" metricValue="1.32842" pageId="13" pageNumber="242" unit="in" value="523.0">523 in</quantity>
ITS, 3/
<quantity box="[1040,1114,806,833]" metricMagnitude="1" metricUnit="m" metricValue="1.1480799999999998" pageId="13" pageNumber="242" unit="in" value="452.0">452 in</quantity>
<emphasis box="[1123,1151,806,832]" italics="true" pageId="13" pageNumber="242">tef</emphasis>
1-α and 3/
<quantity box="[1276,1349,806,832]" metricMagnitude="1" metricUnit="m" metricValue="1.75768" pageId="13" pageNumber="242" unit="in" value="692.0">692 in</quantity>
<emphasis box="[1358,1408,806,832]" italics="true" pageId="13" pageNumber="242">tub2</emphasis>
, no gaps). A strict morphological comparison of our isolates and their phylogenetically related species is presented in
<tableCitation box="[136,252,878,904]" captionStart="TABLE 2" captionStartId="14.[136,216,719,743]" captionTargetPageId="14" captionText="TABLE 2. Comparison of conidia of species identified in this study with closely related taxa." pageId="13" pageNumber="242">
<emphasis bold="true" box="[136,252,878,904]" pageId="13" pageNumber="242">TABLE 2</emphasis>
</tableCitation>
. Furthermore, the
<taxonomicName authorityName="Saussure" authorityYear="1855" box="[468,513,879,905]" class="Insecta" family="Eumenidae" genus="Phi" kingdom="Animalia" order="Hymenoptera" pageId="13" pageNumber="241" phylum="Arthropoda" rank="genus">PHI</taxonomicName>
analysis showed no evidence of genetic recombination between
<taxonomicName box="[1258,1372,878,904]" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" kingdom="Fungi" order="Amphisphaeriales" pageId="13" pageNumber="242" phylum="Ascomycota" rank="species" species="linguae">
<emphasis box="[1258,1372,878,904]" italics="true" pageId="13" pageNumber="242">P. linguae</emphasis>
</taxonomicName>
and
<taxonomicName class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="formosana">
<emphasis italics="true" pageId="13" pageNumber="242">P. formosana</emphasis>
</taxonomicName>
,
<taxonomicName box="[270,446,915,940]" class="Polypodiopsida" family="Polypodiaceae" genus="Pyrrosia" kingdom="Plantae" order="Polypodiales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="nanningensis">
<emphasis box="[270,446,915,940]" italics="true" pageId="13" pageNumber="242">P. nanningensis</emphasis>
</taxonomicName>
,
<taxonomicName box="[460,553,915,940]" class="Magnoliopsida" family="Ericaceae" genus="Pieris" kingdom="Plantae" order="Ericales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="parva">
<emphasis box="[460,553,915,940]" italics="true" pageId="13" pageNumber="242">P. parva</emphasis>
</taxonomicName>
, or
<taxonomicName box="[599,758,914,940]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="13" pageNumber="242" phylum="Tracheophyta" rank="species" species="kandelicola">
<emphasis box="[599,758,914,940]" italics="true" pageId="13" pageNumber="242">P. kandelicola</emphasis>
</taxonomicName>
. Therefore, the collection in this study is designated as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>