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<document id="066A76B7A2BBAC3BED844EAB521BF200" ID-CLB-Dataset="45432" ID-DOI="10.5281/zenodo.210008" ID-GBIF-Dataset="2e21fc45-92e1-4c04-b240-8f726a8408c7" ID-ISSN="1175-5326" ID-Zenodo-Dep="210008" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" checkinTime="1460440014542" checkinUser="plazi" docAuthor="Fend, Steven V. &amp; Lenat, David R." docDate="2012" docId="BC6B87A2FFD6763DFF5BFE6428E1FA28" docLanguage="en" docName="zt03194p067.pdf" docOrigin="Zootaxa 3194" docStyle="DocumentStyle:890A69B780ED73D6DB8551B71C8AC79E.4:Zootaxa.2009-2012.journal_article" docStyleId="890A69B780ED73D6DB8551B71C8AC79E" docStyleName="Zootaxa.2009-2012.journal_article" docStyleVersion="4" docTitle="Eclipidrilus breviatriatus Fend &amp; Lenat, 2012, n. sp." docType="treatment" docVersion="10" lastPageNumber="56" masterDocId="4052FFDAFFD47638FFCCFFD2295CFFCC" masterDocTitle="New Eclipidrilus species (Annelida, Clitellata, Lumbriculidae) from southeastern North America" masterLastPageNumber="67" masterPageNumber="51" pageNumber="53" updateTime="1698332777670" updateUser="ExternalLinkService">
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<mods:title id="11F32164483061DF9F5EFA232FAD2A74">New Eclipidrilus species (Annelida, Clitellata, Lumbriculidae) from southeastern North America</mods:title>
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<mods:name id="4C53C5456DACE057F13EFE493FD31249" type="personal">
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<mods:namePart id="84357396D1BEDECA4D9D8D2448D99047">Fend, Steven V.</mods:namePart>
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<mods:namePart id="D4041D2B0958B6BF2E83F622B8917920">Lenat, David R.</mods:namePart>
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<mods:date id="BF32AC547A4B30FDED842FD86209DB44">2012</mods:date>
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<mods:number id="EF355C6A7A1FBE35BD0AE892A3D0334E">3194</mods:number>
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<mods:identifier id="4DD3381742B0A6A130E013D6DB76A796" type="DOI">10.5281/zenodo.210008</mods:identifier>
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<treatment id="BC6B87A2FFD6763DFF5BFE6428E1FA28" ID-DOI="http://doi.org/10.5281/zenodo.6165716" ID-GBIF-Taxon="119507776" ID-Zenodo-Dep="6165716" LSID="urn:lsid:plazi:treatment:BC6B87A2FFD6763DFF5BFE6428E1FA28" httpUri="http://treatment.plazi.org/id/BC6B87A2FFD6763DFF5BFE6428E1FA28" lastPageId="5" lastPageNumber="56" pageId="2" pageNumber="53">
<subSubSection id="7CD8653FFFD6763AFF5BFE642873FE3E" pageId="2" pageNumber="53" type="nomenclature">
<paragraph id="347D36B4FFD6763AFF5BFE6428A1FE03" blockId="2.[151,509,438,498]" box="[151,509,438,463]" pageId="2" pageNumber="53">
<heading id="6F3581D8FFD6763AFF5BFE6428A1FE03" bold="true" box="[151,509,438,463]" fontSize="10" level="2" pageId="2" pageNumber="53" reason="2">
<emphasis id="06B6EAA6FFD6763AFF5BFE6428A1FE03" bold="true" box="[151,509,438,463]" pageId="2" pageNumber="53">
<taxonomicName id="F3C24D37FFD6763AFF5BFE6428EBFE03" box="[151,439,438,463]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="2" pageNumber="53" phylum="Annelida" rank="species" species="breviatriatus" status="sp. nov.">
<emphasis id="06B6EAA6FFD6763AFF5BFE6428EBFE03" bold="true" box="[151,439,438,463]" italics="true" pageId="2" pageNumber="53">Eclipidrilus breviatriatus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="1D8557DDFFD6763AFE72FE6528A1FE03" box="[446,509,439,463]" pageId="2" pageNumber="53" rank="species">n. sp.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
<paragraph id="347D36B4FFD6763AFF5BFE082873FE3E" blockId="2.[151,509,438,498]" box="[151,303,474,498]" pageId="2" pageNumber="53">
(
<figureCitation id="ACF92A31FFD6763AFF52FE082879FE3E" box="[158,293,474,498]" captionStart-0="FIGURE 1" captionStart-1="FIGURE 2" captionStartId-0="3.[151,250,1882,1905]" captionStartId-1="4.[151,250,1886,1909]" captionTargetBox-0="[236,1337,212,1844]" captionTargetBox-1="[151,1436,196,1849]" captionTargetId-0="figure@3.[220,1349,145,1855]" captionTargetId-1="figure@4.[151,1436,113,1849]" captionTargetPageId-0="3" captionTargetPageId-1="4" captionText-0="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." captionText-1="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri-0="https://zenodo.org/record/210009/files/figure.png" httpUri-1="https://zenodo.org/record/210010/files/figure.png" pageId="2" pageNumber="53">Figures 12</figureCitation>
)
</paragraph>
</subSubSection>
<subSubSection id="7CD8653FFFD6763AFF5BFDF12AAFFD06" pageId="2" pageNumber="53" type="materials_examined">
<paragraph id="347D36B4FFD6763AFF5BFDF12D5BFDF6" blockId="2.[151,1436,546,1974]" box="[151,1031,546,571]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF5BFDF12851FDF7" bold="true" box="[151,269,547,571]" pageId="2" pageNumber="53">
<typeStatus id="EB798816FFD6763AFF5BFDF12854FDF7" box="[151,264,547,571]" pageId="2" pageNumber="53" type="holotype">Holotype</typeStatus>
:
</emphasis>
<collectionCode id="52D3AE71FFD6763AFED8FDF02839FDF6" LSID="urn:lsid:biocol.org:col:34871" box="[276,357,546,570]" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="2" pageNumber="53">USNM</collectionCode>
1156956. A whole, slide-mounted worm in
<collectingCountry id="4CD57624FFD6763AFC99FDF02AF5FDF6" box="[853,937,546,570]" name="Canada" pageId="2" pageNumber="53">Canada</collectingCountry>
balsam.
</paragraph>
<paragraph id="347D36B4FFD6763AFF05FD952A2DFD4E" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF05FD952831FD93" bold="true" box="[201,365,582,607]" pageId="2" pageNumber="53">
<typeStatus id="EB798816FFD6763AFF05FD95285EFD93" box="[201,258,583,607]" pageId="2" pageNumber="53">Type</typeStatus>
locality:
</emphasis>
North Carolina, Richmond Co., unnamed tributary to Naked Creek, at Forest Service road upstream of SR 1003,
<date id="407C1074FFD6763AFE5EFDB828F2FD4E" box="[402,430,618,642]" pageId="2" pageNumber="53" value="2009-01-28">28</date>
January 2009. Collected by D.R. Lenat.
</paragraph>
<paragraph id="347D36B4FFD6763AFF05FD5D2AAFFD06" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF05FD5D2816FD6B" bold="true" box="[201,330,655,679]" pageId="2" pageNumber="53">
<typeStatus id="EB798816FFD6763AFF05FD5D2819FD6B" box="[201,325,655,679]" pageId="2" pageNumber="53" type="paratype">Paratypes</typeStatus>
:
</emphasis>
From the
<typeStatus id="EB798816FFD6763AFE08FD5D28A8FD6B" box="[452,500,655,679]" pageId="2" pageNumber="53">type</typeStatus>
locality.
<collectionCode id="52D3AE71FFD6763AFDACFD5C2BEDFD6A" LSID="urn:lsid:biocol.org:col:34871" box="[608,689,654,678]" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="2" pageNumber="53">USNM</collectionCode>
1156957-59:
<date id="407C1074FFD6763AFC9DFD5C2D58FD6A" box="[849,1028,654,678]" pageId="2" pageNumber="53" value="2010-03-18">18 March 2010</date>
, 1 whole mount.
<date id="407C1074FFD6763AFB02FD5C2CDCFD6A" box="[1230,1408,654,678]" pageId="2" pageNumber="53" value="2010-03-25">25 March 2010</date>
, 1 sagittally sectioned, 1 dissected and slide mounted. Collected by D.R. Lenat.
</paragraph>
</subSubSection>
<subSubSection id="7CD8653FFFD6763AFF05FD052CD0FD22" box="[201,1420,726,751]" pageId="2" pageNumber="53" type="etymology">
<paragraph id="347D36B4FFD6763AFF05FD052CD0FD22" blockId="2.[151,1436,546,1974]" box="[201,1420,726,751]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF05FD05280FFD23" bold="true" box="[201,339,727,751]" pageId="2" pageNumber="53">Etymology:</emphasis>
named for the very short atrial ducts (
<emphasis id="06B6EAA6FFD6763AFCCCFD042A18FD22" box="[768,836,726,750]" italics="true" pageId="2" pageNumber="53">brevis</emphasis>
, L.—short), compared to other
<taxonomicName id="F3C24D37FFD6763AFB64FD042C70FD22" box="[1192,1324,726,750]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="2" pageNumber="53" phylum="Annelida" rank="genus">
<emphasis id="06B6EAA6FFD6763AFB64FD042C70FD22" box="[1192,1324,726,750]" italics="true" pageId="2" pageNumber="53">Eclipidrilus</emphasis>
</taxonomicName>
species.
</paragraph>
</subSubSection>
<subSubSection id="7CD8653FFFD6763AFF05FD282D46FC6E" pageId="2" pageNumber="53" type="materials_examined">
<paragraph id="347D36B4FFD6763AFF05FD282D46FC6E" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF05FD2828DBFCDF" bold="true" box="[201,391,762,787]" pageId="2" pageNumber="53">Other material:</emphasis>
North Carolina, Carteret Co., Pettiford Creek at Millis Road,
<date id="407C1074FFD6763AFB8AFD282DBBFCDE" box="[1094,1255,762,786]" pageId="2" pageNumber="53" value="2004-03-01">1 March 2004</date>
, 1 dissected.
<date id="407C1074FFD6763AFA4CFD282849FCFA" pageId="2" pageNumber="53" value="2008-04-22">22 April 2008</date>
, 2 whole mounts.
<date id="407C1074FFD6763AFE29FCCC2B98FCFA" box="[485,708,798,822]" pageId="2" pageNumber="53" value="2009-09-29">29 September 2009</date>
, 2 whole mounts. Hoke Co., Flat Creek at Manchester Road,
<date id="407C1074FFD6763AFA4CFCCC286CFC96" pageId="2" pageNumber="53" value="2010-01-25">25 January 2010</date>
, 3 whole mounts.
<date id="407C1074FFD6763AFE37FC902B9FFC96" box="[507,707,834,858]" pageId="2" pageNumber="53" value="2010-02-18">18 February 2010</date>
, 1 whole mount.
<date id="407C1074FFD6763AFC48FC902D6EFC96" box="[900,1074,834,858]" pageId="2" pageNumber="53" value="2010-03-25">25 March 2010</date>
, 1 whole mount. Richmond Co., unnamed tributary to Naked Creek at Forest Service road upstream of SR 1003,
<date id="407C1074FFD6763AFBD8FCB42D6CFCB2" box="[1044,1072,870,894]" pageId="2" pageNumber="53" value="2010-03-10">10</date>
March 2010, 1 whole mount.
<date id="407C1074FFD6763AFA4CFCB4287EFC6E" pageId="2" pageNumber="53" value="2010-03-25">25 March 2010</date>
, 1 whole mount, 1 sagittally sectioned. All collected by D.R. Lenat.
</paragraph>
</subSubSection>
<subSubSection id="7CD8653FFFD6763DFF05FC7C2801FBF8" lastPageId="5" lastPageNumber="56" pageId="2" pageNumber="53" type="description">
<paragraph id="347D36B4FFD6763AFF05FC7C28DCFB0F" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">
<emphasis id="06B6EAA6FFD6763AFF05FC7C2807FC0B" bold="true" box="[201,347,942,967]" pageId="2" pageNumber="53">Description.</emphasis>
Small worms, length (preserved)
<quantity id="F33A9B51FFD6763AFD2CFC7C2A12FC0A" box="[736,846,942,966]" metricMagnitude="-2" metricUnit="m" metricValue="1.2" metricValueMax="1.5" metricValueMin="0.9" pageId="2" pageNumber="53" unit="mm" value="12.0" valueMax="15.0" valueMin="9.0">915 mm</quantity>
, 6574 segments; width 0.420.55 mm in X, maximum width to 0.55 mm. Segmentation distinct in external view, but usually obscured in clitellum; secondary segmentation weak, usually visible as a narrow anterior ring in X or IXX, then in a few post-clitellar segments (
<figureCitation id="ACF92A31FFD6763AFAA2FC2429FDFBFE" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="2" pageNumber="53">Fig. 1</figureCitation>
AB). Clitellum prominent in XXII. Chaetae sigmoid, simple-pointed, with nodulus 2940 % of chaeta length from tip (
<figureCitation id="ACF92A31FFD6763AFECDFBEC2863FB9A" box="[257,319,1086,1110]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="2" pageNumber="53">Fig. 1</figureCitation>
CD). Chaeta length 86127 μm; length and proportions similar in anterior and posterior segments. Dorsal and ventral chaetae approximately equal in length; within each bundle, the outer (more lateral) chaeta may be slightly shorter than the inner. Prostomium rounded or conical, usually shorter than wide, length about 0.18 0.22 mm; prolobous.
</paragraph>
<paragraph id="347D36B4FFD6763AFF05FB1C286CF982" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">Brain in the peristomium, strongly lobed. Pharynx in IIIV; dorsal wall with columnar, ciliated cells; ventral wall very thin, with cuboidal, non-ciliated cells. Pharyngeal glands extensive in VVII or VIII. Longitudinal muscle layer 2036 μm thick in preclitellar segments; circular muscle layer 57 μm. Septum 1/2 inconspicuous; 2/3 and 3/4 thin; septa thick (about 5 μm) and distinct in other segments. Epidermis 1015 μm thick anterior to clitellum; to about 2536 μm in clitellum; to 30 μm in prostomium. No lateral blood vessels in middle or posterior segments. Chloragogen cells begin in VII or VIII. First nephridia usually paired on 6/7, the next usually on 12/13; nephridia in few posterior segments, paired or on one side only. Each nephridium has a small anteseptal funnel and a granular, ovate postseptal thickening 5090 μm long; the posterior duct forms a loop that extends ventrally, entering one or more posterior segments, and terminating in a short ectal duct in the originating segment. Nephropores anterior to ventral chaetae simple, inconspicuous; ectal ducts may be slightly expanded near pores, but without distinct vesicles.</paragraph>
<paragraph id="347D36B4FFD6763AFF05F9882B27F87A" blockId="2.[151,1436,546,1974]" pageId="2" pageNumber="53">
Spermathecae paired in IX; pores inconspicuous, on ventral chaetal lines, just behind the ventral chaetae (1/3 1/2 the distance to posterior septum); pores may be surrounded by a 5060 μm ring of slightly thickened epidermis; distinct accessory glands absent (
<figureCitation id="ACF92A31FFD6763AFDC3F9702B0FF976" box="[527,595,1698,1722]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="2" pageNumber="53">Fig. 2</figureCitation>
B, D). Spermathecae have a cylindrical ectal duct, usually slightly inflated at the ectal end; ental end well differentiated from the ovate ampulla (
<figureCitation id="ACF92A31FFD6763AFC0CF9142D5CF912" box="[960,1024,1734,1758]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="2" pageNumber="53">Fig. 1</figureCitation>
EF, 2AB). Ectal duct 80110 μm long, 2230 μm wide, with columnar epithelium, and a narrow (about 5 μm) lumen; may be surrounded by a thin (1215 μm) layer of glandular cells near the pore. Ectal end of duct rounded, or terminating in a small, conical papilla within a shallow sac (
<figureCitation id="ACF92A31FFD6763AFE2AF8E02B77F886" box="[486,555,1842,1866]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="2" pageNumber="53">Fig. 2</figureCitation>
B, D). Spermathecal ampullae entirely in IX; ovate and compact, 90200 μm long by 60140 μm wide, sometimes with the ental end narrowed (
<figureCitation id="ACF92A31FFD6763AFC44F8842A9BF8A2" box="[904,967,1878,1902]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="2" pageNumber="53">Fig. 1</figureCitation>
F). Ampullar epithelium thin (24 μm) in the ectal 1/3, but entally becomes thicker (to 36 μm) with large, irregular cells (
<figureCitation id="ACF92A31FFD6763AFBDBF8A82D3DF85E" box="[1047,1121,1914,1938]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="2" pageNumber="53">Figs. 1</figureCitation>
E, 2C). In mated specimens the sperm is in a loose bundle (
<figureCitation id="ACF92A31FFD6763AFE3FF84C2B69F87A" box="[499,565,1950,1974]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="2" pageNumber="53">Fig. 2</figureCitation>
AC).
</paragraph>
<caption id="60BD663CFFD7763BFF5BF8882812F827" httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="3" pageNumber="54" targetBox="[236,1337,212,1844]" targetPageId="3">
<paragraph id="347D36B4FFD7763BFF5BF8882812F827" blockId="3.[151,1437,1882,2027]" pageId="3" pageNumber="54">
<emphasis id="06B6EAA6FFD7763BFF5BF888284FF8BC" bold="true" box="[151,275,1882,1905]" pageId="3" pageNumber="54">FIGURE 1.</emphasis>
<taxonomicName id="F3C24D37FFD7763BFED5F8882B45F8BC" box="[281,537,1882,1904]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="3" pageNumber="54" phylum="Annelida" rank="species" species="breviatriatus">
<emphasis id="06B6EAA6FFD7763BFED5F8882B45F8BC" box="[281,537,1882,1904]" italics="true" pageId="3" pageNumber="54">Eclipidrilus breviatriatus</emphasis>
</taxonomicName>
(A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). AB, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IXX, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10/11, with associated glands. Scale bars AB 500 μm, CD 50 μm, EG 200 μm.
</paragraph>
</caption>
<caption id="60BD663CFFD0763CFF5BF88C2B1DF81C" httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="4" pageNumber="55" targetBox="[151,1436,196,1849]" targetPageId="4">
<paragraph id="347D36B4FFD0763CFF5BF88C2B1DF81C" blockId="4.[151,1436,1886,2001]" pageId="4" pageNumber="55">
<emphasis id="06B6EAA6FFD0763CFF5BF88C284FF8B8" bold="true" box="[151,275,1886,1909]" pageId="4" pageNumber="55">FIGURE 2.</emphasis>
<taxonomicName id="F3C24D37FFD0763CFED5F88D2B45F8B9" box="[281,537,1887,1909]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="4" pageNumber="55" phylum="Annelida" rank="species" species="breviatriatus">
<emphasis id="06B6EAA6FFD0763CFED5F88D2B45F8B9" box="[281,537,1887,1909]" italics="true" pageId="4" pageNumber="55">Eclipidrilus breviatriatus</emphasis>
</taxonomicName>
(all sagittal sections, specimens from tributary to Naked Creek). AB, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. GJ, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in IJ. KL, sagittal sections of atrial ducts and penes. Scale bars 100 μm.
</paragraph>
</caption>
<paragraph id="347D36B4FFD1763DFF05FF4A2DB2FF3B" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">Testes small in IX and X, extending at most to mid-segment. Ovaries paired in XI; usually extending through XI, often into XII. Sperm sacs extend back as far as XIV; egg sacs may extend to XVI. Female funnels up to 90 140 μm tall, with the posterior side longer than the anterior; female pores intersegmental, on 11/12.</paragraph>
<paragraph id="347D36B4FFD1763DFF05FED62ACAFE03" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">
Male funnels paired on
<date id="407C1074FFD1763DFE1FFED62B20FED0" box="[467,636,260,284]" pageId="5" pageNumber="56" value="2011-09-10" valueMax="2011-09-10" valueMin="2011-09-10">9/10 and 10/11</date>
; anterior and posterior similar in size (height 4070 μm), directed anteriad within IX and X, or the posterior may be directed back within the sperm sac. Both anterior and posterior male funnels simple, conical; functional, with associated sperm. Both anterior and posterior vasa deferentia up to 10 μm wide near the funnels, narrowing to 78 μm near the atria. Vasa deferentia join the atria near the base (ectal end) of the ampulla, then travel diagonally through the atrial muscle layers to join the lumen near the ental end (
<figureCitation id="ACF92A31FFD1763DFAD2FE462C3CFE60" box="[1310,1376,404,428]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
HI). Posterior vasa deferentia enter X directly, without penetrating 10/11.
</paragraph>
<paragraph id="347D36B4FFD1763DFF05FE0E28E0FD23" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">
Male pores paired, in X, on the ventral chaetal line near 10/11 (
<figureCitation id="ACF92A31FFD1763DFC43FE0E2A92FE38" box="[911,974,476,500]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="5" pageNumber="56">Fig. 1</figureCitation>
B, E); may be visible as shallow pits 30 50 μm across, but in contracted worms the pores appear as transverse grooves. The short (1520 μm), broadly conical penis is formed by the narrowed tip of the ectal duct, and may be retracted within the 2040 μm deep sac (
<figureCitation id="ACF92A31FFD1763DFAA2FDF629F9FD93" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
KL). Pyriform accessory glands surround male pores; cells granular, single or in clumps to 60120 μm high, the entire mass to 150 μm wide (
<figureCitation id="ACF92A31FFD1763DFE16FDBE2B45FD48" box="[474,537,620,644]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="5" pageNumber="56">Fig. 1</figureCitation>
G). Additional clusters of large (diameter to 25 μm), apparently vacuolated cells appear to be associated with the atria or the male pores, but are not joined by distinct ducts; these large cell clusters extend to near the apex of the atrium, adjacent to the atrium within X, or in XI if the atrium penetrates the posterior septum (
<figureCitation id="ACF92A31FFD1763DFF3BFD05286AFD23" box="[247,310,727,751]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="5" pageNumber="56">Fig. 1</figureCitation>
E, G:
<date id="407C1074FFD1763DFEB7FD0528C8FD23" box="[379,404,727,751]" pageId="5" pageNumber="56">2I</date>
J).
</paragraph>
<paragraph id="347D36B4FFD1763DFF05FD2E2801FBF8" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">
Atrium may extend into XI. Ectal duct indistinct and short (length 4084 μm, width 2538 μm), gradually widening at the ampulla (
<figureCitation id="ACF92A31FFD1763DFE59FCCD28BEFCFB" box="[405,482,799,823]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="5" pageNumber="56">Figs. 1</figureCitation>
E, G; 2E); musculature more or less transverse-circular; a continuation of ampulla muscle, but not in distinct layers. Atrial duct expands slightly to about 3040 μm in diameter at the base of the penis (
<figureCitation id="ACF92A31FFD1763DFF2FFCB52875FCB3" box="[227,297,871,895]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
K). Atrial ampulla elongate-ovate to club-shaped; length 215355 μm, width at middle 94182 μm. Ampulla with a very thick outer muscle layer, to 4588 μm, composed of about 1216 thin layers of fibers in alternating spirals, oriented at about 5060˚ from the longitudinal axis (
<figureCitation id="ACF92A31FFD1763DFC59FC7D2A85FC0B" box="[917,985,943,967]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
F, G). Epithelium thin and indistinct, as little as 2 μm (at most 78 μm) thick (
<figureCitation id="ACF92A31FFD1763DFDF0FC062B22FC20" box="[572,638,980,1004]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
FH); lumen very narrow, usually less than 5 μm, but may be expanded slightly at ental end (
<figureCitation id="ACF92A31FFD1763DFE41FC252892FBC3" box="[397,462,1015,1039]" captionStart="FIGURE 1" captionStartId="3.[151,250,1882,1905]" captionTargetBox="[236,1337,212,1844]" captionTargetId="figure@3.[220,1349,145,1855]" captionTargetPageId="3" captionText="FIGURE 1. Eclipidrilus breviatriatus (A, C, specimens from Pettiford Creek; B, G, specimens from tributary to Naked Creek; D, E, F, specimens from Flat Creek). A B, lateral view of anterior segments. C, paired chaetae: from left to right, segment III (ventral pair) and XXV (dorsal). D, paired chaetae: X (dorsal) and XII (ventral). E, reproductive segments IX X, from dissected worm, facing left. F, spermatheca. G, atrium penetrating 10 / 11, with associated glands. Scale bars A B 500 μm, C D 50 μm, E G 200 μm." httpUri="https://zenodo.org/record/210009/files/figure.png" pageId="5" pageNumber="56">Fig. 1</figureCitation>
G, 2H). No multicellular prostate glands; ampulla covered with a thin (46 μm) cell layer (
<figureCitation id="ACF92A31FFD1763DFF12FBCE287DFBF8" box="[222,289,1052,1076]" captionStart="FIGURE 2" captionStartId="4.[151,250,1886,1909]" captionTargetBox="[151,1436,196,1849]" captionTargetId="figure@4.[151,1436,113,1849]" captionTargetPageId="4" captionText="FIGURE 2. Eclipidrilus breviatriatus (all sagittal sections, specimens from tributary to Naked Creek). A B, spermathecae. C, spermathecal ampulla. D, spermathecal duct and pore. E, entire atrium. F, transverse-diagonal section of atrial ampulla. G J, sagittal sections of atrial ampullae; lumen expanded in H, large (glandular?) cells next to atrium in I J. K L, sagittal sections of atrial ducts and penes. Scale bars 100 μm." httpUri="https://zenodo.org/record/210010/files/figure.png" pageId="5" pageNumber="56">Fig. 2</figureCitation>
HI).
</paragraph>
</subSubSection>
<subSubSection id="7CD8653FFFD1763DFF0BFB9228E1FA28" pageId="5" pageNumber="56" type="discussion">
<paragraph id="347D36B4FFD1763DFF0BFB922C1FFAC1" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">
<emphasis id="06B6EAA6FFD1763DFF0BFB922867FB94" bold="true" box="[199,315,1088,1112]" pageId="5" pageNumber="56">Remarks.</emphasis>
<taxonomicName id="F3C24D37FFD1763DFE88FB922B3FFB94" box="[324,611,1088,1112]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="5" pageNumber="56" phylum="Annelida" rank="species" species="breviatriatus">
<emphasis id="06B6EAA6FFD1763DFE88FB922B3FFB94" box="[324,611,1088,1112]" italics="true" pageId="5" pageNumber="56">Eclipidrilus breviatriatus</emphasis>
</taxonomicName>
differs from all other
<taxonomicName id="F3C24D37FFD1763DFCA4FB922AB0FB94" box="[872,1004,1088,1112]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="5" pageNumber="56" phylum="Annelida" rank="genus">
<emphasis id="06B6EAA6FFD1763DFCA4FB922AB0FB94" box="[872,1004,1088,1112]" italics="true" pageId="5" pageNumber="56">Eclipidrilus</emphasis>
</taxonomicName>
in having very short atrial ducts; the absence of multicellular prostates is also unusual. However, the very thick, multilayered, spiral musculature of the atrium associates it with the
<emphasis id="06B6EAA6FFD1763DFE2CFB5B28A4FB6C" box="[480,504,1161,1184]" italics="true" pageId="5" pageNumber="56">E.</emphasis>
(
<taxonomicName id="F3C24D37FFD1763DFDC6FB5A2BD7FB6C" box="[522,651,1160,1184]" class="Oligochaeta" family="Acanthodrilidae" genus="Leptodrilus" higherTaxonomySource="CoL" kingdom="Animalia" order="Crassiclitellata" pageId="5" pageNumber="56" phylum="Annelida" rank="genus">
<emphasis id="06B6EAA6FFD1763DFDC6FB5A2BD7FB6C" box="[522,651,1160,1184]" italics="true" pageId="5" pageNumber="56">Leptodrilus</emphasis>
</taxonomicName>
) subgenus, and distinguishes it from other genera in the family. The very small spermathecal ampullae also distinguish it from other congeners, except for
<taxonomicName id="F3C24D37FFD1763DFBA5FB7E2C21FB08" box="[1129,1405,1196,1220]" class="Oligochaeta" family="Lumbriculidae" genus="Eclipidrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Lumbriculida" pageId="5" pageNumber="56" phylum="Annelida" rank="species" species="microthecus" status="sp. nov.">
<emphasis id="06B6EAA6FFD1763DFBA5FB7E2C21FB08" box="[1129,1405,1196,1220]" italics="true" pageId="5" pageNumber="56">Eclipidrilus microthecus</emphasis>
</taxonomicName>
<emphasis id="06B6EAA6FFD1763DFA49FB7E29E4FB24" bold="true" pageId="5" pageNumber="56">
<taxonomicNameLabel id="1D8557DDFFD1763DFA49FB7E29E4FB24" pageId="5" pageNumber="56" rank="species">n. sp.</taxonomicNameLabel>
</emphasis>
(see below). Unmounted worms could usually be distinguished from other small lumbriculids in the region by the short prostomium, prominent septal grooves, and limited secondary segmentation in anterior segments.
</paragraph>
<paragraph id="347D36B4FFD1763DFF05FAC528E1FA28" blockId="5.[151,1437,151,1508]" pageId="5" pageNumber="56">
Of the
<specimenCount id="22C4FD3DFFD1763DFED5FAC528EEFAE3" box="[281,434,1303,1327]" pageId="5" pageNumber="56" type="generic">17 specimens</specimenCount>
of this taxon available for study, 14 were fully mature, with sperm in the spermathecae. Most specimens appeared strongly contracted. Because of the small size of these worms, it was difficult to see details of vasa deferentia, nephridia, and blood vessels. Distinct prostate glands were never seen; although a diffuse mass of transparent, apparently vacuolated cells was often visible adjacent to the atrial ampullae, they did not appear to be attached to the atria. One of the atria was missing in two specimens, but the remaining atrium retained its normal lateral position.
</paragraph>
</subSubSection>
</treatment>
</document>