251 lines
30 KiB
XML
251 lines
30 KiB
XML
<document id="0889FACA4515ED093CAB867F02808424" ID-CLB-Dataset="21475" ID-DOI="10.3161/15081109ACC2017.19.1.003" ID-GBIF-Dataset="4e1384ef-ef30-4f65-95df-aa5eb3f09571" ID-ISSN="1733-5329" ID-Zenodo-Dep="3944816" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" checkinTime="1594740973654" checkinUser="valdenar" docAuthor="Volleth, Marianne, Son, Nguyen Truong, Wu, Yi, Li, Yuchun, Yu, Wenhua, Lin, Liang-Kong, Arai, Satoru, Trifonov, Vladimir, Liehr, Thomas & Harada, Masashi" docDate="2017" docId="03D5879EFFE5C32439537C321E94F9A9" docLanguage="en" docName="ActaChiropterologica.19.1.41-50.pdf.imf" docOrigin="Acta Chiropterologica 19 (1)" docStyle="DocumentStyle:D17EC282BABB8B0847763DCC9F0C3514.3:ActaChiropterol.2016-.journal_article" docStyleId="D17EC282BABB8B0847763DCC9F0C3514" docStyleName="ActaChiropterol.2016-.journal_article" docStyleVersion="3" docTitle="Rhinolophus formosae Sanborn 1939" docType="treatment" docVersion="8" lastPageNumber="45" masterDocId="FFECFFE6FFE1C3213A7A7E561A7FFFCD" masterDocTitle="Comparative chromosomal studies in Rhinolophus formosae and R. luctus from China and Vietnam: elevation of R. l. lanosus to species rank" masterLastPageNumber="50" masterPageNumber="41" pageNumber="44" updateTime="1698843622043" updateUser="ExternalLinkService" zenodo-license-document="CLOSED">
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<mods:title id="79F55AFDDA9B564BBF3FB009E8EFA1E3">Comparative chromosomal studies in Rhinolophus formosae and R. luctus from China and Vietnam: elevation of R. l. lanosus to species rank</mods:title>
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<mods:namePart id="D1DB30A7A3BA09FA52F5CB9BA25A141A">Volleth, Marianne</mods:namePart>
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<mods:affiliation id="9EC9F2C430D2C68AD5BF3D870F20CDFF">Department of Human Genetics, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany</mods:affiliation>
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<mods:namePart id="BD8BE75020FEF0BF97A4DBB723E7D540">Son, Nguyen Truong</mods:namePart>
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<mods:affiliation id="62115A4F904C907B37C0D84C3E7D81E4">Department of Vertebrate Zoology, Institute of Ecology and Biological Resources and Graduate University of Sciences and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam</mods:affiliation>
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<mods:namePart id="5FB7B7A99A4C7542A8C52ADEEEC9C361">Wu, Yi</mods:namePart>
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<mods:affiliation id="605290D985C902704B775EECC0596CB3">College of Life Sciences, Guangzhou University, 230 Wei Huan Xi Road, Guangzhou, 510006, China</mods:affiliation>
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<mods:namePart id="301FB8B1921F3E448D1943529782755A">Li, Yuchun</mods:namePart>
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<mods:affiliation id="C26FCFA4481F78D421E4E504C7C3A0AF">Marine College, Shandong University, Weihai, 264209, China</mods:affiliation>
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<mods:namePart id="A3E0E2CCEF730E5D4F6A98B14921F5E8">Yu, Wenhua</mods:namePart>
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<mods:affiliation id="2BFEC31BFFF81AFC9734A48C233898E3">College of Life Sciences, Guangzhou University, 230 Wei Huan Xi Road, Guangzhou, 510006, China</mods:affiliation>
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<mods:namePart id="2A4915D6E12CBDC59E3B61AAFB00D0E6">Lin, Liang-Kong</mods:namePart>
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<mods:affiliation id="8C28F08A50DD75421C83BB91686D7970">Laboratory of Wildlife Ecology, Department of Biology, Tunghai University, No. 1727, Sec. 4. Taiwan Boulevard, Xitun District, Taichung 40704, Taiwan</mods:affiliation>
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<mods:namePart id="07409080FBF01775D64C07608445B9AE">Arai, Satoru</mods:namePart>
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<mods:affiliation id="55FA1AF42B195596F9EAD8A548E5D82F">Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Toyama 1 - 23 - 1, Shinjuku, Tokyo 162 - 8640, Japan</mods:affiliation>
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<mods:namePart id="18F000ACCB0FDBCB5FCAF1C27CD7C55F">Trifonov, Vladimir</mods:namePart>
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<mods:affiliation id="641E67F0DF5287F74AFD8A69833BD7C4">Institute of Human Genetics, Jena University Hospital, Friedrich-Schiller University, Kollegiengasse 10, 07743 Jena, Germany</mods:affiliation>
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<mods:namePart id="F5C462909E30831359592A5540ADD015">Liehr, Thomas</mods:namePart>
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<mods:affiliation id="D32A9BABE5D830703307A0FBB2624361">Institute of Molecular and Cellular Biology, Siberian Branch of RAS, 8 Ac. Lavrentieva Avenue, Novosibirsk 630090, Russia</mods:affiliation>
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<mods:name id="59664481B6A541113A89BA7C2953C169" type="personal">
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<mods:namePart id="76F19AB476F39AA367472919D7BCA867">Harada, Masashi</mods:namePart>
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<mods:affiliation id="6D300153CD0C8891C9C6F236113CA5CD">Laboratory Animal Center, Graduate School of Medicine, Osaka City University, Abeno, Osaka 545 - 8585, Japan Corresponding author: E-mail: Marianne. Volleth @ med. ovgu. de</mods:affiliation>
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<mods:nameIdentifier id="794B10E69FBCF3A5435272505B69971F" type="email">olleth@med.ovgu.de</mods:nameIdentifier>
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<mods:title id="D8227B9D4B68B6850C67DC0E26420A72">Acta Chiropterologica</mods:title>
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<mods:part id="3813E46693B177131C0143D8B0306F54">
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<mods:date id="BDC27D68E53DCA7A6BC151B0AA17109A">2017</mods:date>
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<mods:number id="A398BB20769D497BFEED504DE3A23985">2017-06-01</mods:number>
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<mods:number id="3553F6A2DEA773060CE763B0E2602E85">19</mods:number>
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<mods:number id="BF45A6A3D1DB6FBC7F622C5BEDF11664">1</mods:number>
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<mods:start id="1E722F331D88BCFF1DD7A05F1A0C943E">41</mods:start>
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<mods:classification id="1E5C87E9F21CF8CEDD7D7016FA8566D5">journal article</mods:classification>
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<mods:identifier id="197B00B49138A6F16B3F4FC2A466D173" type="DOI">10.3161/15081109ACC2017.19.1.003</mods:identifier>
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<mods:identifier id="F1721646FCABC859549E2710691E3749" type="ISSN">1733-5329</mods:identifier>
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<treatment id="03D5879EFFE5C32439537C321E94F9A9" ID-DOI="http://doi.org/10.5281/zenodo.4328142" ID-GBIF-Taxon="165089439" ID-Zenodo-Dep="4328142" LSID="urn:lsid:plazi:treatment:03D5879EFFE5C32439537C321E94F9A9" httpUri="http://treatment.plazi.org/id/03D5879EFFE5C32439537C321E94F9A9" lastPageId="5" lastPageNumber="45" pageId="4" pageNumber="44">
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<subSubSection id="C3666503FFE5C32539537C321E4BFDB3" box="[809,1076,612,638]" pageId="4" pageNumber="44" type="nomenclature">
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<paragraph id="8BC33688FFE5C32539537C321E4BFDB3" blockId="4.[809,1076,612,638]" box="[809,1076,612,638]" pageId="4" pageNumber="44">
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<heading id="D08B81E4FFE5C32539537C321E4BFDB3" box="[809,1076,612,638]" pageId="4" pageNumber="44">
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<taxonomicName id="4C7C4D0BFFE5C32539537C321E4BFDB3" ID-CoL="795R6" authorityName="Sanborn" authorityYear="1939" box="[809,1076,612,638]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="4" pageNumber="44" phylum="Chordata" rank="species" species="formosae">Rhinolophus formosae</taxonomicName>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C3666503FFE5C32439357CF71E94F9A9" lastPageId="5" lastPageNumber="45" pageId="4" pageNumber="44" type="description">
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<paragraph id="8BC33688FFE5C32539357CF71FF1FC27" blockId="4.[809,1422,673,1002]" pageId="4" pageNumber="44">
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Two males and one female from
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<collectingCountry id="F36B7618FFE5C3253E8B7CF71F38FD76" box="[1265,1351,673,699]" name="Taiwan" pageId="4" pageNumber="44">Taiwan</collectingCountry>
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were studied cytogenetically. All specimens showed a karyotype with 2
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<emphasis id="B908EA9AFFE5C3253E717CB31E65FD32" box="[1035,1050,741,767]" italics="true" pageId="4" pageNumber="44">n</emphasis>
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= 52 and a FNa = 60 (
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<figureCitation id="13472A0DFFE5C3253F4E7CB31F01FD32" box="[1332,1406,741,767]" captionStart="FIG" captionStartId="7.[151,164,947,968]" captionTargetBox="[419,1154,183,918]" captionTargetId="figure@7.[383,1190,173,937]" captionTargetPageId="7" captionText="FIG. 6. G-banded karyotype of a male R. formosae. Numbers to the right of each chromosome pair indicate homology to A. stoliczkanus (AST) chromosomal arms as revealed by FISH with AST probes and G-band comparison. On the left of each chromosomal pair, homology to chromosomal arms or segments of the vespertilionid M. myotis (MMY) is given. The inset shows" figureDoi="http://doi.org/10.5281/zenodo.3944834" httpUri="https://zenodo.org/record/3944834/files/figure.png" pageId="4" pageNumber="44">Fig. 6</figureCitation>
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). There were five bi-armed and 19 acrocentric autosomal pairs. In addition, the smallest, dot-like pair no. 25 is likely also bi-armed. Due to the minuteness of this element, the metacentric condition was visible only in a small percentage of metaphase spreads. Therefore, this chromosome was counted as one arm only for the FNa. The medium-sized X chromosome
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</paragraph>
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<caption id="DF036600FFE5C3253953793E1FF2F854" ID-DOI="http://doi.org/10.5281/zenodo.3944820" ID-Zenodo-Dep="3944820" httpUri="https://zenodo.org/record/3944820/files/figure.png" pageId="4" pageNumber="44" startId="4.[809,822,1896,1917]" targetBox="[827,1413,1091,1878]" targetPageId="4">
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<paragraph id="8BC33688FFE5C3253953793E1FF2F854" blockId="4.[809,1421,1896,1945]" pageId="4" pageNumber="44">
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FIG. 2. Metaphase spreads of
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<taxonomicName id="4C7C4D0BFFE5C3253E38793E1EAEF8B0" box="[1090,1233,1896,1917]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="4" pageNumber="44" phylum="Chordata" rank="species" species="luctoides">
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<emphasis id="B908EA9AFFE5C3253E38793E1E2EF8B0" box="[1090,1105,1896,1917]" italics="true" pageId="4" pageNumber="44">R</emphasis>
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. cf.
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<emphasis id="B908EA9AFFE5C3253E01793E1EAEF8B0" box="[1147,1233,1896,1917]" italics="true" pageId="4" pageNumber="44">luctoides</emphasis>
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</taxonomicName>
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after CBG-staining (A) and silver-staining (B), respectively. The arrows in B
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</paragraph>
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</caption>
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<caption id="DF036600FFE5C32539C979F71F7CF87B" ID-DOI="http://doi.org/10.5281/zenodo.3944822" ID-Zenodo-Dep="3944822" box="[947,1283,1953,1974]" httpUri="https://zenodo.org/record/3944822/files/figure.png" pageId="4" pageNumber="44" startId="4.[947,1023,1953,1974]" targetBox="[827,1413,1091,1878]" targetPageId="4">
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<paragraph id="8BC33688FFE5C32539C979F71F7CF87B" blockId="4.[947,1283,1953,1974]" box="[947,1283,1953,1974]" pageId="4" pageNumber="44">indicate the active NORs on pair 8</paragraph>
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</caption>
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<caption id="DF036600FFE4C3243AED7A5E1FF1FB42" ID-DOI="http://doi.org/10.5281/zenodo.3944824" ID-Zenodo-Dep="3944824" httpUri="https://zenodo.org/record/3944824/files/figure.png" pageId="5" pageNumber="45" startId="5.[151,164,1032,1053]" targetBox="[152,1421,174,1012]" targetPageId="5">
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<paragraph id="8BC33688FFE4C3243AED7A5E1FF1FB42" blockId="5.[151,1422,1032,1167]" pageId="5" pageNumber="45">
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FIG. 3. FISH examples showing the hybridization patterns of
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<taxonomicName id="4C7C4D0BFFE4C324397E7A5E19E8FBD0" baseAuthorityName="Dobson" baseAuthorityYear="1871" box="[772,919,1032,1053]" class="Mammalia" family="Hipposideridae" genus="Aselliscus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="stoliczkanus">
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<emphasis id="B908EA9AFFE4C324397E7A5E19E8FBD0" box="[772,919,1032,1053]" italics="true" pageId="5" pageNumber="45">A. stoliczkanus</emphasis>
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</taxonomicName>
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(AST) chromosome-specific painting probes onto metaphase spreads of
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<taxonomicName id="4C7C4D0BFFE4C3243B167A731B82FBF7" box="[364,509,1061,1082]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="luctoides">
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<emphasis id="B908EA9AFFE4C3243B167A731BFEFBF7" box="[364,385,1061,1082]" italics="true" pageId="5" pageNumber="45">R.</emphasis>
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cf.
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<emphasis id="B908EA9AFFE4C3243BDC7A731B82FBF7" box="[422,509,1061,1082]" italics="true" pageId="5" pageNumber="45">luctoides</emphasis>
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</taxonomicName>
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(A, D),
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<taxonomicName id="4C7C4D0BFFE4C32438357A7318CCFBF7" authorityName="Volleth & Son & Wu & Li & Yu & Lin & Arai & Trifonov & Liehr & Harada" authorityYear="2017" box="[591,691,1061,1082]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="lanosus">
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<emphasis id="B908EA9AFFE4C32438357A7318CCFBF7" box="[591,691,1061,1082]" italics="true" pageId="5" pageNumber="45">R. lanosus</emphasis>
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</taxonomicName>
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(B, E) and
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<taxonomicName id="4C7C4D0BFFE4C324395E7A7319E7FBF7" authorityName="Sanborn" authorityYear="1939" box="[804,920,1061,1082]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="formosae">
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<emphasis id="B908EA9AFFE4C324395E7A7319E7FBF7" box="[804,920,1061,1082]" italics="true" pageId="5" pageNumber="45">R. formosae</emphasis>
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</taxonomicName>
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(C, F). A–C — hybridization signals of AST1 (red) and AST8 (green) meet in chromosome 5 in
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<taxonomicName id="4C7C4D0BFFE4C32438317A1718A2FB9B" box="[587,733,1089,1110]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="luctoides">
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<emphasis id="B908EA9AFFE4C32438317A171825FB9B" box="[587,602,1089,1110]" italics="true" pageId="5" pageNumber="45">R</emphasis>
|
||
. cf.
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<emphasis id="B908EA9AFFE4C32438FC7A1718A2FB9B" box="[646,733,1089,1110]" italics="true" pageId="5" pageNumber="45">luctoides</emphasis>
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</taxonomicName>
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(A) and
|
||
<taxonomicName id="4C7C4D0BFFE4C324394C7A1719E5FB9B" authorityName="Volleth & Son & Wu & Li & Yu & Lin & Arai & Trifonov & Liehr & Harada" authorityYear="2017" box="[822,922,1089,1110]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="lanosus">
|
||
<emphasis id="B908EA9AFFE4C324394C7A1719E5FB9B" box="[822,922,1089,1110]" italics="true" pageId="5" pageNumber="45">R. lanosus</emphasis>
|
||
</taxonomicName>
|
||
(B) but not in
|
||
<taxonomicName id="4C7C4D0BFFE4C3243E547A171EDDFB9B" authorityName="Sanborn" authorityYear="1939" box="[1070,1186,1089,1110]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="formosae">
|
||
<emphasis id="B908EA9AFFE4C3243E547A171EDDFB9B" box="[1070,1186,1089,1110]" italics="true" pageId="5" pageNumber="45">R. formosae</emphasis>
|
||
</taxonomicName>
|
||
(C). In B and C AST X (yellow) was applied in addition. D, E — hybridization signals of AST2 (green) and AST5 (red) meet in chromosome 3 of
|
||
<taxonomicName id="4C7C4D0BFFE4C3243F227A0B1A91FB42" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="luctoides">
|
||
<emphasis id="B908EA9AFFE4C3243F227A0B1F18FBBF" box="[1368,1383,1117,1138]" italics="true" pageId="5" pageNumber="45">R</emphasis>
|
||
. cf.
|
||
<emphasis id="B908EA9AFFE4C3243AED7A2C1A91FB42" box="[151,238,1146,1167]" italics="true" pageId="5" pageNumber="45">luctoides</emphasis>
|
||
</taxonomicName>
|
||
(D), but not in
|
||
<taxonomicName id="4C7C4D0BFFE4C3243BF17A2C1B8FFB42" authorityName="Volleth & Son & Wu & Li & Yu & Lin & Arai & Trifonov & Liehr & Harada" authorityYear="2017" box="[395,496,1146,1167]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="lanosus">
|
||
<emphasis id="B908EA9AFFE4C3243BF17A2C1B8FFB42" box="[395,496,1146,1167]" italics="true" pageId="5" pageNumber="45">R. lanosus</emphasis>
|
||
</taxonomicName>
|
||
(E), where AST2 (green) is found in combination with AST1 (red) on chromosome 4 instead.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF036600FFE4C3243B1E7AC01EBFFB66" ID-DOI="http://doi.org/10.5281/zenodo.3944826" ID-Zenodo-Dep="3944826" box="[356,1216,1174,1195]" httpUri="https://zenodo.org/record/3944826/files/figure.png" pageId="5" pageNumber="45" startId="5.[356,369,1174,1195]" targetBox="[152,1421,174,1012]" targetPageId="5">
|
||
<paragraph id="8BC33688FFE4C3243B1E7AC01EBFFB66" blockId="5.[356,1216,1174,1195]" box="[356,1216,1174,1195]" pageId="5" pageNumber="45">
|
||
F — chromosome 1 of
|
||
<taxonomicName id="4C7C4D0BFFE4C324383D7AC018C4FB66" authorityName="Sanborn" authorityYear="1939" box="[583,699,1174,1195]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="formosae">
|
||
<emphasis id="B908EA9AFFE4C324383D7AC018C4FB66" box="[583,699,1174,1195]" italics="true" pageId="5" pageNumber="45">R. formosae</emphasis>
|
||
</taxonomicName>
|
||
showed homology to AST1 (green) and AST12 (red)
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BC33688FFE4C3243AED7B4B1857F878" blockId="5.[151,764,1309,1973]" pageId="5" pageNumber="45">
|
||
is submetacentric and the dot-like Y chromosome is probably bi-armed. Concerning the amount of centromeric heterochromatin, the acrocentric pairs differed from the bi-armed ones. In contrast to the faint staining of centromeric regions of bi-armed pairs 1 to 5 and 25, the acrocentric chromosomes showed dark stained pericentromeric regions after CBG-banding, which were also GTG-, QFQ- and DAPI-positive (
|
||
<figureCitation id="13472A0DFFE4C3243ADA787C1A93F989" box="[160,236,1578,1604]" captionStart="FIG" captionStartId="8.[151,164,1695,1716]" captionTargetBox="[162,754,330,1665]" captionTargetId="figure@8.[162,754,315,1666]" captionTargetPageId="8" captionText="FIG. 7. Metaphase spreads of R. formosae after CBG-banding (A, B) and silver-staining (C). A — Centromeric heterochromatin is clearly visible at acrocentric chromosomes but only faintly stained at the metacentric ones. The arrows point to the smallest autosomal pair no. 25 which is presumably bi-armed. B — The C-banded X chromosomes of the female R. formosae showed the usual appearance for the family without a large hetero-chromatic block. The arrows indicate pair 25. C — Silver-stained metaphase plate with arrows indicating the NORs" figureDoi="http://doi.org/10.5281/zenodo.3944840" httpUri="https://zenodo.org/record/3944840/files/figure.png" pageId="5" pageNumber="45">Fig. 7</figureCitation>
|
||
A–B). The amount of centromeric heterochromatin of the X chromosome was similar to that of other
|
||
<taxonomicName id="4C7C4D0BFFE4C3243A87783B1BEEF94A" authorityName="Lacepede" authorityYear="1799" box="[253,401,1645,1671]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="genus">
|
||
<emphasis id="B908EA9AFFE4C3243A87783B1BEEF94A" box="[253,401,1645,1671]" italics="true" pageId="5" pageNumber="45">Rhinolophus</emphasis>
|
||
</taxonomicName>
|
||
species and not enlarged as in
|
||
<taxonomicName id="4C7C4D0BFFE4C3243AED78D81B35F965" box="[151,330,1678,1704]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="luctoides">
|
||
<emphasis id="B908EA9AFFE4C3243AED78D81ACEF965" box="[151,177,1678,1704]" italics="true" pageId="5" pageNumber="45">R.</emphasis>
|
||
cf.
|
||
<emphasis id="B908EA9AFFE4C3243A9A78D81B35F965" box="[224,330,1678,1704]" italics="true" pageId="5" pageNumber="45">luctoides</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C7C4D0BFFE4C3243BFC78D8187DF965" authorityName="Volleth & Son & Wu & Li & Yu & Lin & Arai & Trifonov & Liehr & Harada" authorityYear="2017" box="[390,514,1678,1704]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="lanosus">
|
||
<emphasis id="B908EA9AFFE4C3243BFC78D8187DF965" box="[390,514,1678,1704]" italics="true" pageId="5" pageNumber="45">R. lanosus</emphasis>
|
||
</taxonomicName>
|
||
. The Y chromosome of
|
||
<taxonomicName id="4C7C4D0BFFE4C3243AC378E61B37F907" authorityName="Sanborn" authorityYear="1939" box="[185,328,1712,1738]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="formosae">
|
||
<emphasis id="B908EA9AFFE4C3243AC378E61B37F907" box="[185,328,1712,1738]" italics="true" pageId="5" pageNumber="45">R. formosae</emphasis>
|
||
</taxonomicName>
|
||
was hardly distinguishable from the autosomal pair 25 after CBG-banding and showed no clear centromeric heterochromatin (
|
||
<figureCitation id="13472A0DFFE4C324382778A518C4F8C0" box="[605,699,1779,1805]" captionStart="FIG" captionStartId="8.[151,164,1695,1716]" captionTargetBox="[162,754,330,1665]" captionTargetId="figure@8.[162,754,315,1666]" captionTargetPageId="8" captionText="FIG. 7. Metaphase spreads of R. formosae after CBG-banding (A, B) and silver-staining (C). A — Centromeric heterochromatin is clearly visible at acrocentric chromosomes but only faintly stained at the metacentric ones. The arrows point to the smallest autosomal pair no. 25 which is presumably bi-armed. B — The C-banded X chromosomes of the female R. formosae showed the usual appearance for the family without a large hetero-chromatic block. The arrows indicate pair 25. C — Silver-stained metaphase plate with arrows indicating the NORs" figureDoi="http://doi.org/10.5281/zenodo.3944840" httpUri="https://zenodo.org/record/3944840/files/figure.png" pageId="5" pageNumber="45">Fig. 7A</figureCitation>
|
||
). The secondary constriction of chromosomal
|
||
<figureCitation id="13472A0DFFE4C324380A794318BDF8E2" box="[624,706,1813,1839]" captionStart="at" captionStartId="8.[411,428,1950,1971]" captionText="at pair 18" pageId="5" pageNumber="45">pair 18</figureCitation>
|
||
(homologous to MMY21) was shown to bear active NORs by silver-staining (
|
||
<figureCitation id="13472A0DFFE4C3243BAA790E184EF8BF" box="[464,561,1880,1906]" captionStart="FIG" captionStartId="8.[151,164,1695,1716]" captionTargetBox="[162,754,330,1665]" captionTargetId="figure@8.[162,754,315,1666]" captionTargetPageId="8" captionText="FIG. 7. Metaphase spreads of R. formosae after CBG-banding (A, B) and silver-staining (C). A — Centromeric heterochromatin is clearly visible at acrocentric chromosomes but only faintly stained at the metacentric ones. The arrows point to the smallest autosomal pair no. 25 which is presumably bi-armed. B — The C-banded X chromosomes of the female R. formosae showed the usual appearance for the family without a large hetero-chromatic block. The arrows indicate pair 25. C — Silver-stained metaphase plate with arrows indicating the NORs" figureDoi="http://doi.org/10.5281/zenodo.3944840" httpUri="https://zenodo.org/record/3944840/files/figure.png" pageId="5" pageNumber="45">Fig. 7C</figureCitation>
|
||
). Analysis of 20 metaphase spreads of one male specimen revealed a frequency of 2.0 NORs per cell.
|
||
</paragraph>
|
||
<paragraph id="8BC33688FFE4C32439357B4A1E94F9A9" blockId="5.[809,1422,1308,1636]" pageId="5" pageNumber="45">
|
||
The complete set of AST painting probes and some selected MMY probes (MMY8, 14, 23) were applied on
|
||
<taxonomicName id="4C7C4D0BFFE4C32439D17B091E46FAB4" authorityName="Sanborn" authorityYear="1939" box="[939,1081,1375,1401]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="formosae">
|
||
<emphasis id="B908EA9AFFE4C32439D17B091E46FAB4" box="[939,1081,1375,1401]" italics="true" pageId="5" pageNumber="45">R. formosae</emphasis>
|
||
</taxonomicName>
|
||
. The results are given on the G-banded karyogram (
|
||
<figureCitation id="13472A0DFFE4C3243E487BD71E08FA56" box="[1074,1143,1409,1435]" captionStart="FIG" captionStartId="7.[151,164,947,968]" captionTargetBox="[419,1154,183,918]" captionTargetId="figure@7.[383,1190,173,937]" captionTargetPageId="7" captionText="FIG. 6. G-banded karyotype of a male R. formosae. Numbers to the right of each chromosome pair indicate homology to A. stoliczkanus (AST) chromosomal arms as revealed by FISH with AST probes and G-band comparison. On the left of each chromosomal pair, homology to chromosomal arms or segments of the vespertilionid M. myotis (MMY) is given. The inset shows" figureDoi="http://doi.org/10.5281/zenodo.3944834" httpUri="https://zenodo.org/record/3944834/files/figure.png" pageId="5" pageNumber="45">Fig. 6</figureCitation>
|
||
) and examples of FISH experiments are shown in
|
||
<figureCitation id="13472A0DFFE4C3243E157BF41F50FA71" box="[1135,1327,1442,1468]" captionStart="FIG" captionStartId="5.[151,164,1032,1053]" captionTargetBox="[152,1421,174,1012]" captionTargetId="figure@5.[162,1410,175,1007]" captionTargetPageId="5" captionText="FIG. 3. FISH examples showing the hybridization patterns of A. stoliczkanus (AST) chromosome-specific painting probes onto metaphase spreads of R. cf. luctoides (A, D), R. lanosus (B, E) and R. formosae (C, F). A–C — hybridization signals of AST1 (red) and AST8 (green) meet in chromosome 5 in R. cf. luctoides (A) and R. lanosus (B) but not in R. formosae (C). In B and C AST X (yellow) was applied in addition. D, E — hybridization signals of AST2 (green) and AST5 (red) meet in chromosome 3 of R. cf. luctoides (D), but not in R. lanosus (E), where AST2 (green) is found in combination with AST1 (red) on chromosome 4 instead." figureDoi="http://doi.org/10.5281/zenodo.3944824" httpUri="https://zenodo.org/record/3944824/files/figure.png" pageId="5" pageNumber="45">
|
||
Fig. 3C and
|
||
<specimenCount id="9D7AFD01FFE4C3243F747BF41F50FA71" box="[1294,1327,1442,1468]" pageId="5" pageNumber="45" type="generic">3F</specimenCount>
|
||
</figureCitation>
|
||
. Of the chromosomal pairs 1 to 5, only two, i.e., 4 and 5, show the same combination of chromosomal arms as found in
|
||
<taxonomicName id="4C7C4D0BFFE4C32439C778511E34F9EC" box="[957,1099,1543,1569]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="luctoides">
|
||
<emphasis id="B908EA9AFFE4C32439C778511E34F9EC" box="[957,1099,1543,1569]" italics="true" pageId="5" pageNumber="45">R. luctoides</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C7C4D0BFFE4C3243E2478511EA3F9EC" authorityName="Volleth & Son & Wu & Li & Yu & Lin & Arai & Trifonov & Liehr & Harada" authorityYear="2017" box="[1118,1244,1543,1569]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="lanosus">
|
||
<emphasis id="B908EA9AFFE4C3243E2478511EA3F9EC" box="[1118,1244,1543,1569]" italics="true" pageId="5" pageNumber="45">R. lanosus</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C7C4D0BFFE4C3243F6678511FF9F9EC" authorityName="Gray" authorityYear="1842" box="[1308,1414,1543,1569]" class="Mammalia" family="Rhinolophidae" genus="Rhinolophus" kingdom="Animalia" order="Chiroptera" pageId="5" pageNumber="45" phylum="Chordata" rank="species" species="morio">
|
||
<emphasis id="B908EA9AFFE4C3243F6678511FF9F9EC" box="[1308,1414,1543,1569]" italics="true" pageId="5" pageNumber="45">R. morio</emphasis>
|
||
</taxonomicName>
|
||
. Pairs 1 to 3 show a unique combination hitherto found in no other rhinolophid species.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |