150 lines
12 KiB
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150 lines
12 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.75.57192" ID-GBIF-Dataset="70ebf204-5c89-4782-8357-853357218fdd" ID-PMC="PMC7669824" ID-Pensoft-Pub="1314-4049-75-31" ID-Pensoft-UUID="C7E17CB78AA959F4AC1E9E9A425599B2" ID-PubMed="33223920" ModsDocID="1314-4049-75-31" checkinTime="1604986494284" checkinUser="pensoft" docAuthor="Lv, Shi-Long, Chai, Chun-Yue, Wang, Yun, Yan, Zhen-Li & Hui, Feng-Li" docDate="2020" docId="749F60683E6A58F6801B77710D2D60A9" docLanguage="en" docName="MycoKeys 75: 31-49" docOrigin="MycoKeys 75" docSource="http://dx.doi.org/10.3897/mycokeys.75.57192" docTitle="Spathaspora jiuxiensis C. Y. Chai & F. L. Hui 2020, sp. nov." docType="treatment" docVersion="4" id="C7E17CB78AA959F4AC1E9E9A425599B2" lastPageNumber="31" masterDocId="C7E17CB78AA959F4AC1E9E9A425599B2" masterDocTitle="Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China" masterLastPageNumber="49" masterPageNumber="31" pageNumber="31" updateTime="1668136577485" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Lv, Shi-Long</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Chai, Chun-Yue</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wang, Yun</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Yan, Zhen-Li</mods:namePart>
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<mods:affiliation>State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co. Ltd., Nanyang 473000, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hui, Feng-Li</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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<mods:nameIdentifier type="email">fenglihui@yeah.net</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2020</mods:date>
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<mods:detail type="volume">
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<mods:number>75</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>31</mods:start>
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<mods:end>49</mods:end>
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</mods:extent>
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</mods:part>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.75.57192</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.75.57192</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-75-31</mods:identifier>
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<mods:identifier type="Pensoft-UUID">C7E17CB78AA959F4AC1E9E9A425599B2</mods:identifier>
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<treatment ID-GBIF-Taxon="168961852" LSID="urn:lsid:plazi:treatment:749F60683E6A58F6801B77710D2D60A9" httpUri="http://treatment.plazi.org/id/749F60683E6A58F6801B77710D2D60A9" lastPageNumber="31" pageId="0" pageNumber="31">
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<subSubSection pageId="0" pageNumber="31" type="nomenclature">
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<paragraph pageId="0" pageNumber="31">
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<taxonomicName LSID="749F6068-3E6A-58F6-801B-77710D2D60A9" authority="C. Y. Chai & F. L. Hui" authorityName="C. Y. Chai & F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Debaryomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora jiuxiensis" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="jiuxiensis" status="sp. nov.">Spathaspora jiuxiensis C.Y. Chai & F.L. Hui</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="31">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Morphology of Spathaspora jiuxiensis (NYNU 17416, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure4" httpUri="https://binary.pensoft.net/fig/473339" pageId="0" pageNumber="31">Figure 4</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="materials_examined">
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<paragraph pageId="0" pageNumber="31">Type.</paragraph>
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<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2017, K.F. Liu & L. Zhang (holotype, NYNU 17416T preserved in a metabolically-inactive state), ex-holotype: CICC 33264; CBS 15226.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="etymology">
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<paragraph pageId="0" pageNumber="31">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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<taxonomicName genus="Jiuxiensis" lsidName="Jiuxiensis" pageId="0" pageNumber="31" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="31">Jiuxiensis</emphasis>
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</taxonomicName>
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refers to Jiuxi Mountain, the mountain from which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="description">
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<paragraph pageId="0" pageNumber="31">Description.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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In YM broth after 3 days at 25 °C, cells are ovoid to elongate (3-6
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<normalizedToken originalValue="×">x</normalizedToken>
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3.5-9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) and occur singly or in pairs (Fig.
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Morphology of Spathaspora jiuxiensis (NYNU 17416, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure4" httpUri="https://binary.pensoft.net/fig/473339" pageId="0" pageNumber="31">4a</figureCitation>
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); pseudohyphae are present. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. After 3 days of growth on YM agar at 25 °C, colonies are white to cream-coloured, butyrous and smooth with entire margins. After 12 days at 25 °C on Dalmau plate culture on CM agar, pseudohyphae and true hyphae are formed (Fig.
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Morphology of Spathaspora jiuxiensis (NYNU 17416, holotype) a budding cells and pseudohyphae on YM broth after 3 d b true hyphae with blastoconidia on CM agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure4" httpUri="https://binary.pensoft.net/fig/473339" pageId="0" pageNumber="31">4b</figureCitation>
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). Asci or signs of conjugation were not seen on the sporulation media used. Glucose and maltose are weakly fermented. Xylose fermentation is negative using Durham tubes, but ethanol is produced from xylose when determined with alcohol oxidase and peroxidase tests. Glucose, d-glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, methyl
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<normalizedToken originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
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, cellobiose, salicin, arbutin, melezitose, inulin, ribitol, d-glucitol, d-mannitol, 2-keto-d-gluconate, dl-lactate, succinate and ethanol are assimilated. No growth occurs with galactose, l-sorbose, l-arabinose, d-arabinose, l-rhamnose, melibiose, lactose, raffinose, glycerol, erythritol, xylitol, galactitol,
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<emphasis italics="true" pageId="0" pageNumber="31">myo</emphasis>
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-inositol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, d-glucuronate, citrate, l-arabinitol or methanol. For the assimilation of nitrogen compounds, growth on l-lysine, glucosamine or d-tryptophan is present, whereas growth on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine or imidazole is absent. Growth is observed at 35 °C, but not at 37 °C. Growth in the presence of 0.01% cycloheximide is present, but growth in the presence of 0.1% cycloheximide, 10% NaCl plus 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.
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</paragraph>
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<caption doi="10.3897/mycokeys.75.57192.figure4" httpUri="https://binary.pensoft.net/fig/473339" pageId="0" pageNumber="31" start="Figure 4" startId="F4">
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<paragraph pageId="0" pageNumber="31">
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<emphasis bold="true" pageId="0" pageNumber="31">Figure 4.</emphasis>
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Morphology of
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<taxonomicName authorityName="C. Y. Chai & F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Saccharomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora jiuxiensis" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="jiuxiensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Spathaspora jiuxiensis</emphasis>
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</taxonomicName>
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(NYNU 17416, holotype)
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<emphasis bold="true" pageId="0" pageNumber="31">a</emphasis>
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budding cells and pseudohyphae on YM broth after 3 d
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<emphasis bold="true" pageId="0" pageNumber="31">b</emphasis>
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true hyphae with blastoconidia on CM agar after 14 d. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="additional isolate examined">
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<paragraph pageId="0" pageNumber="31">Additional isolate examined.</paragraph>
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<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Honghe Prefecture, Luxi County, in rotting wood in Jiuxi Mountain Forest Park, July 2017, K.F. Liu & L. Zhang, NYNU 17417.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="notes">
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<paragraph pageId="0" pageNumber="31">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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The two strains, both representing
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<taxonomicName lsidName="Sp. jiuxiensis" pageId="0" pageNumber="31" rank="species" species="jiuxiensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. jiuxiensis</emphasis>
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</taxonomicName>
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, cluster in a well-supported clade in the phylogenetic analysis and are closely related to
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<taxonomicName lsidName="Sp. parajiuxiensis" pageId="0" pageNumber="31" rank="species" species="parajiuxiensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. parajiuxiensis</emphasis>
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</taxonomicName>
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. The nucleotide differences between these two new species were 1.4% substitutions in the D1/D2 domain and 4.6% substitutions in the ITS region (
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<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.11.007" author="Groenewald, DVM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="31" pagination="91 - 105" refId="B9" refString="Groenewald, DVM, Szoeke, S, Cardinali, G, Eberhardt, U, Stielow, B, Vries, M de, Verkleij, MJM, Crous, PW, Boekhout, T, Robert, V, 2016. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Studies in Mycology 85: 91 - 105, DOI: https://doi.org/10.1016/j.simyco.2016.11.007" title="DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation." url="https://doi.org/10.1016/j.simyco.2016.11.007" volume="85" year="2016">Groenewald et al. 2016</bibRefCitation>
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). These two sister species can also be differentiated by a few physiological characteristics;
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<taxonomicName lsidName="Sp. jiuxiensis" pageId="0" pageNumber="31" rank="species" species="jiuxiensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. jiuxiensis</emphasis>
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</taxonomicName>
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can assimilate dl-lactate and
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<taxonomicName lsidName="Sp. parajiuxiensis" pageId="0" pageNumber="31" rank="species" species="parajiuxiensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. parajiuxiensis</emphasis>
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</taxonomicName>
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can grow at 37 °C.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |