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122 lines
13 KiB
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<document ID-DOI="http://dx.doi.org/10.3897/zookeys.1015.54387" ID-GBIF-Dataset="7f0a1826-ba89-4ecb-adbb-275211293c87" ID-PMC="PMC7878468" ID-Pensoft-Pub="1313-2970-1015-1" ID-Pensoft-UUID="AF1641758561525C8D40BBF3F895FA8A" ID-PubMed="33613041" ID-ZooBank="F6BD92139DB74564AA003C61B2F43B2D" ModsDocID="1313-2970-1015-1" checkinTime="1612497320802" checkinUser="pensoft" docAuthor="Abe, Hirokazu & Sato-Okoshi, Waka" docDate="2021" docId="1AD75CAF2760535E8A6600177F5E01F6" docLanguage="en" docName="ZooKeys 1015: 1-86" docOrigin="ZooKeys 1015" docPubDate="2021-02-04" docSource="http://dx.doi.org/10.3897/zookeys.1015.54387" docTitle="Spio sp. 1" docType="treatment" docVersion="5" id="AF1641758561525C8D40BBF3F895FA8A" lastPageNumber="1" masterDocId="AF1641758561525C8D40BBF3F895FA8A" masterDocTitle="Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan" masterLastPageNumber="86" masterPageNumber="1" pageNumber="1" updateTime="1668149773418" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Abe, Hirokazu</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7753-9368</mods:nameIdentifier>
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<mods:affiliation>Department of Biology, Center for Liberal Arts & Sciences, Iwate Medical University, Idaidori 1 - 1 - 1, Yahaba-cho, Shiwa-gun, Iwate 028 - 3694, Japan</mods:affiliation>
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<mods:nameIdentifier type="email">habe@iwate-med.ac.jp</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sato-Okoshi, Waka</mods:namePart>
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<mods:affiliation>Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Aramaki-Aza-Aoba 468 - 1, Aoba-ku, Sendai 980 - 8572, Japan</mods:affiliation>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:title>ZooKeys</mods:title>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-02-04</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>1015</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>1</mods:start>
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<mods:end>86</mods:end>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/zookeys.1015.54387</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/zookeys.1015.54387</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1313-2970-1015-1</mods:identifier>
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<mods:identifier type="ZooBank">F6BD92139DB74564AA003C61B2F43B2D</mods:identifier>
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<mods:identifier type="Pensoft-UUID">AF1641758561525C8D40BBF3F895FA8A</mods:identifier>
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<treatment ID-GBIF-Taxon="177212519" LSID="urn:lsid:plazi:treatment:1AD75CAF2760535E8A6600177F5E01F6" httpUri="http://treatment.plazi.org/id/1AD75CAF2760535E8A6600177F5E01F6" lastPageNumber="1" pageId="0" pageNumber="1">
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<subSubSection pageId="0" pageNumber="1" type="nomenclature">
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<paragraph pageId="0" pageNumber="1">
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<taxonomicName LSID="1AD75CAF-2760-535E-8A66-00177F5E01F6" authority="sp. 1" authorityName="sp. 1" class="Polychaeta" family="Spionidae" genus="Spio" higherTaxonomySource="treatment-meta" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">Spio sp. 1</taxonomicName>
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<figureCitation captionStart="Figure 9" captionStartId="F10" captionText="Figure 9. Light micrographs showing the morphologies of living spionid larvae of genera Pseudopolydora and Spio A, B Pseudopolydora aff. achaeta, dorsal view of 12 - chaetiger (A) and 25 - chetiger larvae (B) C Pseudopolydora cf. kempi, dorsolateral view of 12 - chetiger larva D Pseudopolydora paucibranchiata, dorsal view of 13 - chaetiger larva E, F Pseudopolydora cf. reticulata, dorsal view of 17 - chaetiger (E) and 16 - chaetiger larvae (F) G, H Pseudopolydora tsubaki, dorsal view of 5 - chaetiger (G) and 11 - chaetiger larvae (H) I Pseudopolydora sp., dorsal view of 7 - chaetiger larva J, K Spio sp. 1, dorsal view of 8 - chaetiger (J) and lateral view of 12 - chaetiger larvae (K) L-N Spio sp. 2, dorsal view of 10 - chaetiger (L) and 17 - chaetiger larvae (M), and 17 - chaetiger metamorphosing larvae (N). Scale bars: 300 μm." figureDoi="10.3897/zookeys.1015.54387.figure9" httpUri="https://binary.pensoft.net/fig/505196" pageId="0" pageNumber="1">Fig. 9J, K</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="larval morphology">
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<paragraph pageId="0" pageNumber="1">Larval morphology.</paragraph>
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<paragraph pageId="0" pageNumber="1">Overall larval shape slender and weakly fusiform. Larval chaetae on first chaetiger fairly long. Prostomium round anteriorly. Small patches of black pigment on peristomium ventrally. Three pairs of black eyes present, most lateral pairs double-eyes. Distinct black melanophore absent, rows of faint transverse band-shaped black pigmentation on dorsum from chaetiger IV onwards. Pharynx exhibits weak dark or brownish pigmentation. Gut yellow-green in color due to ingested food.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="remarks">
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<paragraph pageId="0" pageNumber="1">Remarks.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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Adult individuals of this species were collected from Rishiri Island, northern Japan, in July and August 2017. These specimens were previously identified as
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<taxonomicName lsidName="S. arndti" pageId="0" pageNumber="1" rank="species" species="arndti">
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<emphasis italics="true" pageId="0" pageNumber="1">S. arndti</emphasis>
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</taxonomicName>
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<normalizedToken originalValue="Meißner">Meissner</normalizedToken>
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, Bick & Bastrop, 2011 (
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<bibRefCitation author="Abe, H" journalOrPublisher="Rishiri Studies" pageId="0" pageNumber="1" pagination="15 - 27" publicationUrl="http://riishiri.sakura.ne.jp/Sites/RS/archive/382019/3803.pdf" refId="B6" refString="Abe, H, Tomioka, S, Kobayashi, G, Itoh, H, 2019c. Spionidae (Annelida) from Rishiri Island, Northern Japan. Rishiri Studies 38: 15 - 27, http://riishiri.sakura.ne.jp/Sites/RS/archive/382019/3803.pdf" title="Spionidae (Annelida) from Rishiri Island, Northern Japan." url="http://riishiri.sakura.ne.jp/Sites/RS/archive/382019/3803.pdf" volume="38" year="2019 c">Abe et al. 2019c</bibRefCitation>
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) since adult morphology agreed. Although 18S rRNA gene sequence obtained in the present study 100% match with that of
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<taxonomicName lsidName="S. arndti" pageId="0" pageNumber="1" rank="species" species="arndti">
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<emphasis italics="true" pageId="0" pageNumber="1">S. arndti</emphasis>
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</taxonomicName>
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(FR823434, 1761/1761 bp), because the 16S rRNA gene sequences were different (6.7%, 30/451 bp), the species reported here is referred to
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio</emphasis>
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</taxonomicName>
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sp. 1. The 16S rRNA gene sequence of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio</emphasis>
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</taxonomicName>
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sp. 1 was rather more similar (96.1%, 298/310 bp) to that of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio</emphasis>
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</taxonomicName>
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sp. 2573 from Russia (KT200126), but conspecificity of these two is unclear. A few planktonic larvae of this species were collected from Onagawa Bay only in May 2011. The larvae and adults were confirmed to match (18S: 1762/1762, 16S: 466/467 bp) using molecular data (Fig.
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Maximum Likelihood tree inferred from nuclear 18 S and mitochondrial 16 S rRNA gene sequences of spionids obtained from Japan in the present and previous studies (provided in Table 1). The gene sequences of adult and larval spionid polychaetes are indicated by solid squares and circles in front of each species name, respectively. SH-aLRT / approximate Bayes support / ultrafast bootstrap support values of ≥ 80 %, ≥ 0.95, ≥ 95 %, respectively are given beside the respective nodes. Nodes with red circles indicate triple high support values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Laonome sp. and Sabella pavonina Savigny, 1822 obtained from DDBJ / EMBL / GenBank were used for outgroup rooting." figureDoi="10.3897/zookeys.1015.54387.figure2" httpUri="https://binary.pensoft.net/fig/505188" pageId="0" pageNumber="1">2</figureCitation>
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).
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</paragraph>
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<paragraph pageId="0" pageNumber="1">
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The absence of distinct black melanophores in larvae of this species differentiates them from those of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio</emphasis>
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</taxonomicName>
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sp. 2 (see below). Slight dorsal pigmentation was also reported in adelphophagic benthic larvae of
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<taxonomicName authorityName="Verrill" authorityYear="1873" class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio setosa" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="setosa">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio setosa</emphasis>
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</taxonomicName>
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Verrill, 1873 sensu
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<bibRefCitation author="Simon, JL" journalOrPublisher="Bulletin of Marine Science" pageId="0" pageNumber="1" pagination="398 - 431" publicationUrl="https://www.ingentaconnect.com/content/umrsmas/bullmar/1967/00000017/00000002/art00015" refId="B195" refString="Simon, JL, 1967. Reproduction and larval development of Spio setosa (Spionidae: Polychaeta). Bulletin of Marine Science 17: 398 - 431, https://www.ingentaconnect.com/content/umrsmas/bullmar/1967/00000017/00000002/art00015" title="Reproduction and larval development of Spio setosa (Spionidae: Polychaeta)." url="https://www.ingentaconnect.com/content/umrsmas/bullmar/1967/00000017/00000002/art00015" volume="17" year="1967">Simon (1967</bibRefCitation>
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,
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<bibRefCitation DOI="https://doi.org/10.2307/1539868" author="Simon, JL" journalOrPublisher="The Biological Bulletin" pageId="0" pageNumber="1" pagination="503 - 515" refId="B196" refString="Simon, JL, 1968. Occurrence of pelagic larvae in Spio setosa Verrill 1873 (Polychaeta: Spionidae). The Biological Bulletin 134: 503 - 515, DOI: https://doi.org/10.2307/1539868" title="Occurrence of pelagic larvae in Spio setosa Verrill 1873 (Polychaeta: Spionidae)." url="https://doi.org/10.2307/1539868" volume="134" year="1968">1968</bibRefCitation>
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), which were essentially unpigmented, and in those of
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<taxonomicName baseAuthorityName="Rioja" baseAuthorityYear="1918" class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio multioculata" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="multioculata">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio multioculata</emphasis>
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</taxonomicName>
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(Rioja, 1918) described by
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<bibRefCitation author="Hannerz, L" journalOrPublisher="Zoologiska bidrag fran Uppsala" pageId="0" pageNumber="1" pagination="1 - 204" refId="B67" refString="Hannerz, L, 1956. Larval development of the polychaete families Spionidae Sars, Disomidae Mensil, and Poecilochaetidae n. fam. in the Gullmar Fjord (Sweden). Zoologiska bidrag fran Uppsala 31: 1 - 204" title="Larval development of the polychaete families Spionidae Sars, Disomidae Mensil, and Poecilochaetidae n. fam. in the Gullmar Fjord (Sweden)." volume="31" year="1956">Hannerz (1956)</bibRefCitation>
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. However, the larval morphologies of these two species are different from that of
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<taxonomicName class="Polychaeta" family="Spionidae" genus="Spio" kingdom="Animalia" lsidName="Spio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Spio</emphasis>
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</taxonomicName>
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sp. 1 in lacking ventral black pigment on the peristomium (in both former species) and long larval chaetae on the first chaetiger (in
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<taxonomicName lsidName="S. setosa" pageId="0" pageNumber="1" rank="species" species="setosa">
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<emphasis italics="true" pageId="0" pageNumber="1">S. setosa</emphasis>
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</taxonomicName>
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), and in having a relatively thickened body shape (in both species).
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |