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<mods:title id="24A6847FD7EC69072BA7309ABDFCA21C">A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae</mods:title>
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DESCRIPTIONOF
<taxonomicName id="4C011396D2270269FB9CFB68550737C0" class="Bacilli" family="Lactobacillaceae" genus="Ligilactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="species" species="araffinosus" status="sp. nov.">
<emphasis id="B975B407D2270269FB9CFB68550737C0" bold="true" italics="true" pageId="40" pageNumber="2822">LIGILACTOBACILLUS ARAFFINOSUS</emphasis>
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<taxonomicName id="4C011396D2270269FC95FB185554361A" box="[828,1110,1260,1284]" class="Bacilli" family="Lactobacillaceae" genus="Ligilactobacillus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="species" species="araffinosus">
<emphasis id="B975B407D2270269FC95FB185554361A" box="[828,1110,1260,1284]" italics="true" pageId="40" pageNumber="2822">Ligilactobacillus araffinosus</emphasis>
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(a.raf.fi.nosus. Gr. pref.
<emphasis id="B975B407D2270269FAE4FB195458361A" box="[1357,1370,1261,1284]" italics="true" pageId="40" pageNumber="2822">a</emphasis>
not; N.L. masc. adj.
<emphasis id="B975B407D2270269FC05FAFF5513363D" box="[940,1041,1291,1315]" italics="true" pageId="40" pageNumber="2822">raffinosus</emphasis>
of raffinose; N.L. masc. adj.
<taxonomicName id="4C011396D2270269FAEBFAFF54B6363D" box="[1346,1460,1291,1315]" class="Bacilli" family="Lactobacillaceae" genus="Ligilactobacillus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="species" species="araffinosus">
<emphasis id="B975B407D2270269FAEBFAFF54B6363D" box="[1346,1460,1291,1315]" italics="true" pageId="40" pageNumber="2822">araffinosus</emphasis>
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, not fermenting raffinose).
</paragraph>
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Basonym:
<taxonomicName id="4C011396D2270269FC04FAAD52AA368E" authority="Fujisawa et al. 1985" authorityName="Fujisawa" authorityYear="1985" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="subSpecies" species="aviarius" subSpecies="araffinosus">
<emphasis id="B975B407D2270269FC04FAAD5590366E" box="[941,1170,1369,1393]" italics="true" pageId="40" pageNumber="2822">Lactobacillus aviarius</emphasis>
subsp.
<emphasis id="B975B407D2270269FB4DFAAD5454366F" box="[1252,1366,1369,1393]" italics="true" pageId="40" pageNumber="2822">araffinosus</emphasis>
Fujisawa
<emphasis id="B975B407D2270269FC95FA8D5265368E" box="[828,871,1400,1424]" italics="true" pageId="40" pageNumber="2822">etal</emphasis>
. 1985
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, 223
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; Fujisawa
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. 1986, (Effective publication: Fujisawa
<emphasis id="B975B407D2270269FC37FA6C52C836B1" box="[926,970,1431,1455]" italics="true" pageId="40" pageNumber="2822">etal</emphasis>
., 1984, 419).
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Placement of
<taxonomicName id="4C011396D2270269FC62FA32555A36C0" box="[971,1112,1478,1502]" class="Bacilli" family="Lactobacillaceae" genus="Ligilactobacillus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="species" species="araffinosus">
<emphasis id="B975B407D2270269FC62FA32555A36C0" box="[971,1112,1478,1502]" italics="true" pageId="40" pageNumber="2822">L. araffinosus</emphasis>
</taxonomicName>
in a separate species is supported by ANI values and digital DNADNA hybridization [
<bibRefCitation id="EF9015E4D2270269FACDFA11548136E3" author="Wittouck S &amp; Wuyts S &amp; Meehan CJ &amp; van Noort V &amp; Lebeer S." box="[1380,1411,1509,1533]" journalOrPublisher="mSystems" pageId="40" pageNumber="2822" part="4" refId="ref59914" refString="10. Wittouck S, Wuyts S, Meehan CJ, van Noort V, Lebeer S. A genome-based species taxonomy of the Lactobacillus Genus Complex. mSystems 2019; 4: e 00264 - 19." title="A genome-based species taxonomy of the Lactobacillus Genus Complex" type="journal volume" year="2019">10</bibRefCitation>
]. The availability of the genome sequences of the type strains of both subspecies revealed that they are only distantly related with an ANI value of 88.98%, substantially below the 9596 % threshold recognised for the species level. Furthermore, their core nucleotide identity (CNI) [
<bibRefCitation id="EF9015E4D2270269FB72F97655FB3584" author="Wittouck S &amp; Wuyts S &amp; Meehan CJ &amp; van Noort V &amp; Lebeer S." box="[1243,1273,1666,1690]" journalOrPublisher="mSystems" pageId="40" pageNumber="2822" part="4" refId="ref59914" refString="10. Wittouck S, Wuyts S, Meehan CJ, van Noort V, Lebeer S. A genome-based species taxonomy of the Lactobacillus Genus Complex. mSystems 2019; 4: e 00264 - 19." title="A genome-based species taxonomy of the Lactobacillus Genus Complex" type="journal volume" year="2019">10</bibRefCitation>
] is 91.1, which is below the 94 % threshold at which two strains are allocated tothe same species. Finally, their
<emphasis id="B975B407D2270269FB3EF93555E735C6" box="[1175,1253,1728,1752]" italics="true" pageId="40" pageNumber="2822">in silico</emphasis>
DDH value is 37.80, whichadditionally supports thatthese two subspeciesshould be recognised as different species [
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].
</paragraph>
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Cells are Gram- positive, non-motile, non-spore-forming rods with rounded ends, usually occurring singly or in short chains.
<taxonomicName id="4C011396D2270269FC23F8985516349A" box="[906,1044,1900,1924]" class="Bacilli" family="Lactobacillaceae" genus="Ligilactobacillus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="40" pageNumber="2822" phylum="Firmicutes" rank="species" species="araffinosus">
<emphasis id="B975B407D2270269FC23F8985516349A" box="[906,1044,1900,1924]" italics="true" pageId="40" pageNumber="2822">L. araffinosus</emphasis>
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strainsferment trehaloseand cellobiose, they do not produce acid from galactose, lactose, melibiose
</paragraph>
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and raffinose [
<bibRefCitation id="EF9015E4D2260268FEB8FF42503233D0" author="Fujisawa T &amp; Shirasaka S &amp; Watabe J &amp; Mitsuoka T." box="[273,304,182,206]" pageId="41" pageNumber="2823" pagination="414 - 420" refId="ref61478" refString="51. Fujisawa T, Shirasaka S, Watabe J, Mitsuoka T. Lactobacillus aviarius sp. nov.: a new species isolated from the intestine of chickens. Syst Appl Microbiol 1984; 5: 414 - 420." type="journal article" year="1984">51</bibRefCitation>
]. The genome size of the type strain is 1.48 Mbp. The mol% G+C content of DNA is 38.1.
</paragraph>
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<paragraph id="8BBE6815D2260268FFD0FEF753603205" blockId="41.[121,610,259,283]" box="[121,610,259,283]" pageId="41" pageNumber="2823">Isolated from the intestine and faeces of birds.</paragraph>
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Thetypestrainis ML2
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= ATCC 43235
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= DSM 20653
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= CCUG 32231
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=CIP 103145
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= JCM 5667
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<paragraph id="8BBE6815D2260268FFD0FE8953BC328B" blockId="41.[121,702,381,405]" box="[121,702,381,405]" pageId="41" pageNumber="2823">Genome sequence accession number: AYYZ00000000.</paragraph>
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16S rRNA gene accession number:
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