treatments-xml/data/03/DB/4E/03DB4E5BFFCDFFECFF7EF82DFB1D6A88.xml
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<document id="A14BCAD3D75EE45AEFEE61F82FF14456" ID-CLB-Dataset="55100" ID-DOI="10.11646/phytotaxa.603.2.4" ID-GBIF-Dataset="f682b8fb-e9a3-410d-8057-12540ad287e3" ID-ISSN="1179-3163" ID-Zenodo-Dep="8157954" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1689662249455" checkinUser="plazi" docAuthor="Liu, Ling-Ling, Feng, Yao, Wei, Quan-Quan, Zhang, Meng, Gu, Xiao-Feng, Liu, Zuo-Yi &amp; Gou, Jiu-Lan" docDate="2023" docId="03DB4E5BFFCDFFECFF7EF82DFB1D6A88" docLanguage="en" docName="phytotaxa.603.2.4.pdf" docOrigin="Phytotaxa 603 (2)" docSource="http://dx.doi.org/10.1094/PDIS-04-22-0755-PDN" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.6:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="6" docTitle="Triplosphaeria guizhouensis L. L. Liu &amp; Z. Y. Liu 2023, sp. nov." docType="treatment" docVersion="2" lastPageNumber="179" masterDocId="FFE23623FFC9FFEAFFF6FFF9FFE46E0D" masterDocTitle="Triplosphaeria guizhouensis sp. nov. (Tetraplosphaeriaceae, Pleosporales), a novel taxon from freshwater habitat in Guizhou Province, China" masterLastPageNumber="183" masterPageNumber="173" pageNumber="177" updateTime="1689662928835" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="AB3DCEF560737F0DF063E892B619AAAA">Triplosphaeria guizhouensis sp. nov. (Tetraplosphaeriaceae, Pleosporales), a novel taxon from freshwater habitat in Guizhou Province, China</mods:title>
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<mods:namePart id="CD9D9CFD3B7BCDC975FAF916622E1CD3">Liu, Ling-Ling</mods:namePart>
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<mods:affiliation id="8074E3A2B4F437CC9278416B4FB07E17">Guizhou Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China &amp; Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China College of Ethnic-Minority Medicine, Guizhou Minzu University, Guiyang, 550025, Guizhou, China &amp; liulingling 1557 @ 163. com; https: // orcid. org / 0000 - 0003 - 0006 - 0938</mods:affiliation>
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<mods:namePart id="A9D9B4B59F361B21546F1950486F3701">Feng, Yao</mods:namePart>
<mods:nameIdentifier id="56D6F53B5FB51156801CA2F450A4B06B" type="ORCID">0000-0002-0888-8775</mods:nameIdentifier>
<mods:affiliation id="CD6F09DE69DDF01C1C64D24DEF26813E">Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China College of Ethnic-Minority Medicine, Guizhou Minzu University, Guiyang, 550025, Guizhou, China &amp; yfeng 0409 @ 163. com; https: // orcid. org / 0000 - 0002 - 0888 - 8775</mods:affiliation>
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<mods:namePart id="0367C39AE716F2B7E627B77EEFD2C2E4">Wei, Quan-Quan</mods:namePart>
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<mods:affiliation id="0AC6E95E55E63870FA4E508F32DCB909">Guizhou Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China &amp; weiquan 0725 @ 163. com; https: // orcid. org / 0000 - 0002 - 1672 - 1588</mods:affiliation>
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<mods:namePart id="72CAD594F8087585D35A32EE06FC9C4B">Zhang, Meng</mods:namePart>
<mods:nameIdentifier id="FC1930237C3D26DF9BC1F630B0EFFF3E" type="ORCID">0000-0002-3312-1402</mods:nameIdentifier>
<mods:affiliation id="CA447B566C9535DBEAF3CFC4BA86EB9C">Guizhou Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China &amp; zhangmeng 1105 @ 163. com; https: // orcid. org / 0000 - 0002 - 3312 - 1402</mods:affiliation>
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<mods:namePart id="4EB699088747BD17C73EB24EC3DEFE23">Gu, Xiao-Feng</mods:namePart>
<mods:nameIdentifier id="AA3A7490617123DB56E395FD3DAF4BB6" type="ORCID">0000-0002-1197-2070</mods:nameIdentifier>
<mods:affiliation id="FCB7EF2D757BCD21AD7408097C008EB0">Guizhou Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China &amp; guxiaofengnky @ 163. com; https: // orcid. org / 0000 - 0002 - 1197 - 2070</mods:affiliation>
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<mods:namePart id="341FC5712A9AB8354B9331522908A3EA">Liu, Zuo-Yi</mods:namePart>
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<mods:affiliation id="49969E845B71B8AC8A3CDBD5BB59EB13">Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China College of Ethnic-Minority Medicine, Guizhou Minzu University, Guiyang, 550025, Guizhou, China &amp; gzliuzuoyi @ 163. com; https: // orcid. org / 0000 - 0001 - 5348 - 8458</mods:affiliation>
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<mods:namePart id="1A0E83340A78346A993ADFE298288246">Gou, Jiu-Lan</mods:namePart>
<mods:nameIdentifier id="1431FDFA55ACAD85535CF8E7639163D6" type="ORCID">0000-0001-6439-158X</mods:nameIdentifier>
<mods:affiliation id="8F73DB7782B7CC613DF298540517E84A">Guizhou Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou, China &amp; gzgoujiulan @ 163. com; https: // orcid. org / 0000 - 0001 - 6439 - 158 X</mods:affiliation>
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<mods:title id="88531FC0252FD77B64E4CDC790C609BD">Phytotaxa</mods:title>
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<mods:date id="E448A427D516EA5F0D83AB41C9A53358">2023</mods:date>
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<mods:number id="47E267B50F435E25134A928F7B806CED">2023-07-18</mods:number>
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<mods:number id="C80598E951F50BB3EED41DDFBA2C2699">603</mods:number>
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<mods:identifier id="19748897EC481FDD565C76D0E700AAE5" type="DOI">10.11646/phytotaxa.603.2.4</mods:identifier>
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<treatment id="03DB4E5BFFCDFFECFF7EF82DFB1D6A88" ID-DOI="http://doi.org/10.5281/zenodo.8157956" ID-Zenodo-Dep="8157956" LSID="urn:lsid:plazi:treatment:03DB4E5BFFCDFFECFF7EF82DFB1D6A88" httpUri="http://treatment.plazi.org/id/03DB4E5BFFCDFFECFF7EF82DFB1D6A88" lastPageId="6" lastPageNumber="179" pageId="4" pageNumber="177">
<subSubSection id="C368ACC6FFCDFFEEFF7EF82DFCD2661F" pageId="4" pageNumber="177" type="nomenclature">
<paragraph id="8BCDFF4DFFCDFFEEFF7EF82DFC6169E3" blockId="4.[136,901,2004,2031]" box="[136,901,2004,2031]" pageId="4" pageNumber="177">
<heading id="D0854821FFCDFFEEFF7EF82DFC6169E3" box="[136,901,2004,2031]" fontSize="11" level="2" pageId="4" pageNumber="177" reason="3">
<taxonomicName id="4C7284CEFFCDFFEEFF7EF82DFD5E69E3" authority="L.L. Liu &amp; Z.Y. Liu" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[136,698,2004,2031]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="4" pageNumber="177" phylum="Ascomycota" rank="species" species="guizhouensis" status="sp. nov.">
<emphasis id="B906235FFFCDFFEEFF7EF82DFE3469E2" bold="true" box="[136,464,2004,2031]" italics="true" pageId="4" pageNumber="177">Triplosphaeria guizhouensis</emphasis>
L.L. Liu &amp; Z.Y. Liu
</taxonomicName>
,
<emphasis id="B906235FFFCDFFEEFD33F82CFCFE69E3" box="[709,794,2005,2030]" italics="true" pageId="4" pageNumber="177">
<taxonomicNameLabel id="A2359E24FFCDFFEEFD33F82CFCFE69E3" box="[709,794,2005,2030]" pageId="4" pageNumber="177" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
<figureCitation id="1349E3C8FFCDFFEEFCD6F82DFC6569E3" box="[800,897,2004,2030]" captionStart="FIGURE 2" captionStartId="5.[136,229,1953,1975]" captionTargetBox="[179,1407,190,1928]" captionTargetId="figure-18@5.[179,1407,190,1928]" captionTargetPageId="5" captionText="FIGURE 2. Triplosphaeria guizhouensis (GZAAS20-0407, holotype). a, b Colonies on natural substrate. cf Conidia bearing 34 appendages. g Germinating conidium. h, i Culture on PDA, h from above, i from below. Scale bars: cf=25 μm. g =50 μm." figureDoi="http://doi.org/10.5281/zenodo.8157960" httpUri="https://zenodo.org/record/8157960/files/figure.png" pageId="4" pageNumber="177">Figure 2</figureCitation>
.
</heading>
</paragraph>
<paragraph id="8BCDFF4DFFCDFFEEFF7EF805FCD2661F" blockId="4.[136,822,2044,2066]" box="[136,822,2044,2066]" pageId="4" pageNumber="177">Index Fungorum number: IF 900370; Facesoffungi number: FoF 10694</paragraph>
</subSubSection>
<caption id="DF0DAFC5FFCCFFEFFF7EF858FAC369D7" ID-DOI="http://doi.org/10.5281/zenodo.8157960" ID-Zenodo-Dep="8157960" httpUri="https://zenodo.org/record/8157960/files/figure.png" pageId="5" pageNumber="178" startId="5.[136,229,1953,1975]" targetBox="[179,1407,190,1928]" targetPageId="5" targetType="figure">
<paragraph id="8BCDFF4DFFCCFFEFFF7EF858FAC369D7" blockId="5.[136,1451,1952,2011]" pageId="5" pageNumber="178">
<emphasis id="B906235FFFCCFFEFFF7EF858FDC369BB" bold="true" box="[136,551,1952,1975]" pageId="5" pageNumber="178">
FIGURE 2.
<taxonomicName id="4C7284CEFFCCFFEFFEFCF859FDC369BB" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[266,551,1952,1974]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="5" pageNumber="178" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCCFFEFFEFCF859FDC369BB" bold="true" box="[266,551,1952,1974]" italics="true" pageId="5" pageNumber="178">Triplosphaeria guizhouensis</emphasis>
</taxonomicName>
</emphasis>
(GZAAS20-0407,
<emphasis id="B906235FFFCCFFEFFD1EF858FCA469BB" bold="true" box="[744,832,1953,1974]" pageId="5" pageNumber="178">holotype</emphasis>
). a, b Colonies on natural substrate. cf Conidia bearing 34 appendages. g Germinating conidium. h, i Culture on PDA, h from above, i from below.
<emphasis id="B906235FFFCCFFEFFC2DF83CFBAC69D7" box="[987,1096,1989,2010]" italics="true" pageId="5" pageNumber="178">Scale bars:</emphasis>
cf=25 μm. g =50 μm.
</paragraph>
</caption>
<subSubSection id="C368ACC6FFCFFFECFF7EFF67FF336C65" pageId="6" pageNumber="179" type="etymology">
<paragraph id="8BCDFF4DFFCFFFECFF7EFF67FB3D6EB5" blockId="6.[136,1452,158,1157]" box="[136,1241,158,185]" pageId="6" pageNumber="179">
Etymology: In reference to the host location,
<collectingRegion id="49B631AFFFCFFFECFD73FF67FCB56EB5" box="[645,849,158,184]" country="China" name="Guizhou" pageId="6" pageNumber="179">Guizhou province</collectingRegion>
, where the
<typeStatus id="54C941EFFFCFFFECFC27FF67FBD76EB5" box="[977,1075,158,184]" pageId="6" pageNumber="179">holotype</typeStatus>
was collected.
</paragraph>
<paragraph id="8BCDFF4DFFCFFFECFF4BFF3BFED46FD5" blockId="6.[136,1452,158,1157]" pageId="6" pageNumber="179">
<emphasis id="B906235FFFCFFFECFF4BFF3BFEC56ED1" box="[189,289,194,220]" italics="true" pageId="6" pageNumber="179">Saprobic</emphasis>
on decaying submerged wood in freshwater habitats.
<emphasis id="B906235FFFCFFFECFC64FF3BFBB06ED1" bold="true" box="[914,1108,194,220]" pageId="6" pageNumber="179">Asexual morph:</emphasis>
<emphasis id="B906235FFFCFFFECFBA8FF3BFB266ED1" box="[1118,1218,194,220]" italics="true" pageId="6" pageNumber="179">Colonies</emphasis>
on natural substrate effuse, consisting of individual conidium scattered over the substrate surface, conspicuous, brown to black.
<emphasis id="B906235FFFCFFFECFACBFF1FFA4F6F0D" box="[1341,1451,230,256]" italics="true" pageId="6" pageNumber="179">Mycelium</emphasis>
mostly immersed, consisting of branched, septate, smooth, subhyaline to pale brown hyphae.
<emphasis id="B906235FFFCFFFECFB59FEF3FAB06F29" box="[1199,1364,266,292]" italics="true" pageId="6" pageNumber="179">Conidiophores</emphasis>
absent.
<emphasis id="B906235FFFCFFFECFF7EFED7FE8E6F45" box="[136,362,302,328]" italics="true" pageId="6" pageNumber="179">Conidiogenous cells</emphasis>
monoblastic.
<emphasis id="B906235FFFCFFFECFDF0FED7FD846F45" box="[518,608,302,328]" italics="true" pageId="6" pageNumber="179">Conidia</emphasis>
solitary, straight or slightly curved, conidia body 18.923 × 89 μm (
<emphasis id="B906235FFFCFFFECFA92FED4FA966F44" box="[1380,1394,301,329]" italics="true" pageId="6" pageNumber="179">x</emphasis>
= 21 × 8.5 μm, n = 15), composed of three or four columns of cells, 57-septate in each column, guttulate, smooth-walled or sometimes verrucose on the base cell, with 34 setose appendages.
<emphasis id="B906235FFFCFFFECFC76FE8FFBED6F9D" box="[896,1033,374,400]" italics="true" pageId="6" pageNumber="179">Appendages</emphasis>
(4.7) 12.618.9 (21.5) long, 11.5 μm wide, divergent, pale brown to brown, 24-septate, straight or slightly flexuous, smooth-walled.
<emphasis id="B906235FFFCFFFECFB0CFE63FA4F6FB9" bold="true" box="[1274,1451,410,436]" pageId="6" pageNumber="179">Sexual morph:</emphasis>
Undetermined.
</paragraph>
<paragraph id="8BCDFF4DFFCFFFECFF4BFE1BFF336C65" blockId="6.[136,1452,158,1157]" pageId="6" pageNumber="179">
<emphasis id="B906235FFFCFFFECFF4BFE1BFE026FF1" bold="true" box="[189,486,482,508]" pageId="6" pageNumber="179">Cultural characteristics:</emphasis>
Conidia germinating on WA and germ tubes produced from appendages within 12 h. Colonies on PDA growing slowly, reaching
<quantity id="4C8A52A8FFCFFFECFD5EFDFFFCC06C2C" box="[680,804,518,545]" metricMagnitude="-2" metricUnit="m" metricValue="2.25" metricValueMax="2.5" metricValueMin="2.0" pageId="6" pageNumber="179" unit="mm" value="22.5" valueMax="25.0" valueMin="20.0">2025 mm</quantity>
DIAM. after one month at 25°C, under dark condition, circular, consisting of a matted felt with velutinous appearance, umbonate, surface of the colony mouse grey, in reverse brown.
</paragraph>
</subSubSection>
<subSubSection id="C368ACC6FFCFFFECFF4BFD8BFB1D6A88" pageId="6" pageNumber="179" type="materials_examined">
<paragraph id="8BCDFF4DFFCFFFECFF4BFD8BFD5D6CD8" blockId="6.[136,1452,158,1157]" pageId="6" pageNumber="179">
<emphasis id="B906235FFFCFFFECFF4BFD8BFE496C81" bold="true" box="[189,429,626,652]" pageId="6" pageNumber="179">Material examined:</emphasis>
<materialsCitation id="3B1AF510FFCFFFECFE4EFD8BFD516CD8" collectingDate="2019-07-16" collectionCode="GZCC" collectorName="L. L. Liu" country="China" elevation="204" latitude="28.416666" location="Chishui river" longLatPrecision="1232" longitude="106.0" municipality="Chishui City" pageId="6" pageNumber="179" specimenCode="GZCC 19-0512" specimenCount="1" stateProvince="Guizhou" typeStatus="holotype">
<collectingCountry id="F365BFDDFFCFFFECFE4EFD8BFDF06C81" box="[440,532,626,652]" name="China" pageId="6" pageNumber="179">CHINA</collectingCountry>
,
<collectingRegion id="49B631AFFFCFFFECFDD5FD8BFD106C81" box="[547,756,626,652]" country="China" name="Guizhou" pageId="6" pageNumber="179">Guizhou Province</collectingRegion>
,
<collectingMunicipality id="6BA96537FFCFFFECFCF2FD8BFC736C81" box="[772,919,626,652]" pageId="6" pageNumber="179">Chishui City</collectingMunicipality>
,
<location id="8EADA996FFCFFFECFC50FD8BFBDA6C81" LSID="urn:lsid:plazi:treatment:03DB4E5BFFCDFFECFF7EF82DFB1D6A88:8EADA996FFCFFFECFC50FD8BFBDA6C81" box="[934,1086,626,652]" country="China" latitude="28.416666" longLatPrecision="1232" longitude="106.0" municipality="Chishui City" name="Chishui river" pageId="6" pageNumber="179" stateProvince="Guizhou">Chishui river</location>
basin,
<geoCoordinate id="EE46998AFFCFFFECFB60FD8DFB126C82" box="[1174,1270,628,655]" degrees="28" direction="north" minutes="25" orientation="latitude" pageId="6" pageNumber="179" precision="925" value="28.416666">28°25N</geoCoordinate>
,
<geoCoordinate id="EE46998AFFCFFFECFAF3FD8DFA866C82" box="[1285,1378,628,655]" degrees="106" direction="east" minutes="0" orientation="longitude" pageId="6" pageNumber="179" precision="925" value="106.0">106°0E</geoCoordinate>
, at an altitude of
<quantity id="4C8A52A8FFCFFFECFEF3FD6FFEB46CBC" box="[261,336,662,689]" metricMagnitude="2" metricUnit="m" metricValue="2.04" pageId="6" pageNumber="179" unit="m" value="204.0">
<elevation id="005F187EFFCFFFECFEF3FD6FFEB46CBC" box="[261,336,662,689]" metricMagnitude="2" metricUnit="m" metricValue="2.04" pageId="6" pageNumber="179" unit="m" value="204.0">204 m</elevation>
</quantity>
, on submerged decaying wood in a stream,
<date id="FFCCD98DFFCFFFECFCBEFD6FFC3B6CBD" box="[840,991,662,688]" pageId="6" pageNumber="179" value="2019-07-16">
<collectingDate id="EF882065FFCFFFECFCBEFD6FFC3B6CBD" box="[840,991,662,688]" pageId="6" pageNumber="179" value="2019-07-16">16 July 2019</collectingDate>
</date>
,
<collectorName id="26879A9BFFCFFFECFC1AFD6EFBAB6CBD" box="[1004,1103,662,689]" pageId="6" pageNumber="179">L.L. Liu</collectorName>
,
<emphasis id="B906235FFFCFFFECFBAAFD6FFB2E6CBD" bold="true" box="[1116,1226,662,688]" pageId="6" pageNumber="179">CS1-18-1</emphasis>
(GZAAS 200407,
<emphasis id="B906235FFFCFFFECFF7EFD43FF0B6CD9" bold="true" box="[136,239,698,724]" pageId="6" pageNumber="179">
<typeStatus id="54C941EFFFCFFFECFF7EFD43FF0B6CD9" box="[136,239,698,724]" pageId="6" pageNumber="179">holotype</typeStatus>
</emphasis>
), ex-type living culture
<specimenCode id="DBD45736FFCFFFECFE08FD43FD516CD8" box="[510,693,698,725]" collectionCode="GZCC" pageId="6" pageNumber="179">GZCC 190512</specimenCode>
</materialsCitation>
.
</paragraph>
<paragraph id="8BCDFF4DFFCFFFECFF4BFD27FB1D6A88" blockId="6.[136,1452,158,1157]" pageId="6" pageNumber="179">
<emphasis id="B906235FFFCFFFECFF4BFD27FEED6CF5" bold="true" box="[189,265,734,760]" pageId="6" pageNumber="179">Notes:</emphasis>
<taxonomicName id="4C7284CEFFCFFFECFEF9FD27FDA86CF5" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[271,588,734,760]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFEF9FD27FDA86CF5" box="[271,588,734,760]" italics="true" pageId="6" pageNumber="179">Triplosphaeria guizhouensis</emphasis>
</taxonomicName>
shares similar morphological characteristics with the
<emphasis id="B906235FFFCFFFECFB6AFD27FAE76CF5" box="[1180,1283,734,760]" italics="true" pageId="6" pageNumber="179">tetraploa</emphasis>
-like anamorph in having cylindrical conidia with columns of cells and apical appendages (
<bibRefCitation id="EFE382BCFFCFFFECFC3FFCFBFB476D11" author="Tanaka, K. &amp; Hirayama, K. &amp; Yonezawa, H. &amp; Hatakeyama, S. &amp; Harada, Y. &amp; Sano, T. &amp; Shirouzu, T. &amp; Hosoya, T." box="[969,1187,770,797]" pageId="6" pageNumber="179" pagination="175 - 209" refId="ref7365" refString="Tanaka, K., Hirayama, K., Yonezawa, H., Hatakeyama, S., Harada, Y., Sano, T., Shirouzu, T. &amp; Hosoya, T. (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with tetraploa-like anamorphs. Studies in Mycology 64: 175 - 209. https: // doi. org / 10.3114 / sim. 2009.64.10" type="journal article" year="2009">
Tanaka
<emphasis id="B906235FFFCFFFECFBD7FCFAFBBD6D11" box="[1057,1113,770,796]" italics="true" pageId="6" pageNumber="179">et al.</emphasis>
, 2009
</bibRefCitation>
). Phylogenetic analyses of combined LSU, ITS, SSU,
<emphasis id="B906235FFFCFFFECFE1CFCDFFDC86D4D" box="[490,556,806,832]" italics="true" pageId="6" pageNumber="179">tef1-α</emphasis>
, and
<emphasis id="B906235FFFCFFFECFD87FCDFFD3C6D4D" box="[625,728,806,832]" italics="true" pageId="6" pageNumber="179">β-tubulin</emphasis>
sequence data indicates that the new strain (GZAAS20-0407) nested within the
<taxonomicName id="4C7284CEFFCFFFECFEAFFCB3FDAC6D69" box="[345,584,842,868]" class="Dothideomycetes" family="Tetraplosphaeriaceae" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="family">Tetraplosphaeriaceae</taxonomicName>
, and was resolved as a monophyletic clade with the genus
<taxonomicName id="4C7284CEFFCFFFECFAF3FCB3FA4F6D69" box="[1285,1451,842,868]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="genus">
<emphasis id="B906235FFFCFFFECFAF3FCB3FA4F6D69" box="[1285,1451,842,868]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
</taxonomicName>
(
<figureCitation id="1349E3C8FFCFFFECFF79FC97FEE86D85" box="[143,268,878,904]" captionStart="FIGURE 1" captionStartId="4.[136,229,1647,1669]" captionTargetBox="[153,1435,187,1618]" captionTargetId="figure-27@4.[151,1435,187,1620]" captionTargetPageId="4" captionText="FIGURE 1. The maximum likelihood (ML) tree for Tetraplosphaeriaceae and related orders is based on the combined LSU, ITS, SSU, tef1-α, and β-tubulin sequence data. Bootstrap support values for maximum likelihood (ML) greater than 75% and Bayesian posterior probabilities greater than 0.95 are indicated above branches as ML BS/PP. The scale bar represents the expected number of changes per site. The tree is rooted with Amniculicola immersa (CBS 123083) and Amniculicola parva (CBS 123092). Ex-type strains are indicated with T. The new taxa are in red bold. Branches with 100% ML BS and 1.0PP were dotted with black dots. Genus are indicated as colored blocks." figureDoi="http://doi.org/10.5281/zenodo.8157958" httpUri="https://zenodo.org/record/8157958/files/figure.png" pageId="6" pageNumber="179">FIGURE 1</figureCitation>
).
<taxonomicName id="4C7284CEFFCFFFECFED0FC97FD816D85" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[294,613,878,904]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFED0FC97FD816D85" box="[294,613,878,904]" italics="true" pageId="6" pageNumber="179">Triplosphaeria guizhouensis</emphasis>
</taxonomicName>
can be distinguished from other
<taxonomicName id="4C7284CEFFCFFFECFC28FC97FB3A6D85" box="[990,1246,878,904]" pageId="6" pageNumber="179">
<emphasis id="B906235FFFCFFFECFC28FC97FB606D85" box="[990,1156,878,904]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
species
</taxonomicName>
in having conidia with ¾ columns and ¾ setose appendages. Moreover, the conidia of
<taxonomicName id="4C7284CEFFCFFFECFC57FC6AFBAB6DA1" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[929,1103,914,940]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFC57FC6AFBAB6DA1" box="[929,1103,914,940]" italics="true" pageId="6" pageNumber="179">T. guizhouensis</emphasis>
</taxonomicName>
on the host plant differs from those of
<taxonomicName id="4C7284CEFFCFFFECFF1EFC4FFE6A6DDD" box="[232,398,950,976]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="genus">
<emphasis id="B906235FFFCFFFECFF1EFC4FFE6A6DDD" box="[232,398,950,976]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
</taxonomicName>
produced under culture conditions in having smaller conidia and shorter appendages (
<tableCitation id="C6F0CAF6FFCFFFECFAA7FC4FFF7D6DF9" captionStart="TABLE 2" captionStartId="7.[136,224,786,812]" captionText="TABLE 2. Synoposis of Triplosphaeria species." pageId="6" pageNumber="179">TABLE 2</tableCitation>
).
<taxonomicName id="4C7284CEFFCFFFECFF5BFC23FE9F6DF8" box="[173,379,986,1013]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="undetermined">
<emphasis id="B906235FFFCFFFECFF5BFC23FEB76DF9" box="[173,339,986,1012]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
sp.
</taxonomicName>
formed smaller conidia on the host plant also (
<tableCitation id="C6F0CAF6FFCFFFECFC7EFC23FC1C6DF9" box="[904,1016,986,1012]" captionStart="TABLE 2" captionStartId="7.[136,224,786,812]" captionText="TABLE 2. Synoposis of Triplosphaeria species." pageId="6" pageNumber="179">TABLE 2</tableCitation>
) (
<bibRefCitation id="EFE382BCFFCFFFECFBF8FC23FB0E6DF9" author="Tanaka, K. &amp; Hirayama, K. &amp; Yonezawa, H. &amp; Hatakeyama, S. &amp; Harada, Y. &amp; Sano, T. &amp; Shirouzu, T. &amp; Hosoya, T." box="[1038,1258,986,1013]" pageId="6" pageNumber="179" pagination="175 - 209" refId="ref7365" refString="Tanaka, K., Hirayama, K., Yonezawa, H., Hatakeyama, S., Harada, Y., Sano, T., Shirouzu, T. &amp; Hosoya, T. (2009) Molecular taxonomy of bambusicolous fungi: Tetraplosphaeriaceae, a new pleosporalean family with tetraploa-like anamorphs. Studies in Mycology 64: 175 - 209. https: // doi. org / 10.3114 / sim. 2009.64.10" type="journal article" year="2009">
Tanaka
<emphasis id="B906235FFFCFFFECFB91FC22FB446DF9" box="[1127,1184,986,1012]" italics="true" pageId="6" pageNumber="179">et al.</emphasis>
, 2009
</bibRefCitation>
). In phylogenetic analysis,
<taxonomicName id="4C7284CEFFCFFFECFF06FC06FE786A15" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[240,412,1022,1048]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFF06FC06FE786A15" box="[240,412,1022,1048]" italics="true" pageId="6" pageNumber="179">T. guizhouensis</emphasis>
</taxonomicName>
is sister to
<taxonomicName id="4C7284CEFFCFFFECFDECFC07FCE56A14" box="[538,769,1022,1049]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Tetraplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="undetermined">
<emphasis id="B906235FFFCFFFECFDECFC07FD3E6A15" box="[538,730,1022,1048]" italics="true" pageId="6" pageNumber="179">Tetraplosphaeria</emphasis>
sp.
</taxonomicName>
(
<collectionCode id="ED636788FFCFFFECFCF9FC07FCB96A15" box="[783,861,1022,1048]" country="Japan" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15135" lsid="urn:lsid:biocol.org:col:15135" name="Hirosaki University" pageId="6" pageNumber="179" type="Herbarium">HHUF</collectionCode>
27481) (
<figureCitation id="1349E3C8FFCFFFECFC37FC07FBFB6A15" box="[961,1055,1022,1048]" captionStart="FIGURE 1" captionStartId="4.[136,229,1647,1669]" captionTargetBox="[153,1435,187,1618]" captionTargetId="figure-27@4.[151,1435,187,1620]" captionTargetPageId="4" captionText="FIGURE 1. The maximum likelihood (ML) tree for Tetraplosphaeriaceae and related orders is based on the combined LSU, ITS, SSU, tef1-α, and β-tubulin sequence data. Bootstrap support values for maximum likelihood (ML) greater than 75% and Bayesian posterior probabilities greater than 0.95 are indicated above branches as ML BS/PP. The scale bar represents the expected number of changes per site. The tree is rooted with Amniculicola immersa (CBS 123083) and Amniculicola parva (CBS 123092). Ex-type strains are indicated with T. The new taxa are in red bold. Branches with 100% ML BS and 1.0PP were dotted with black dots. Genus are indicated as colored blocks." figureDoi="http://doi.org/10.5281/zenodo.8157958" httpUri="https://zenodo.org/record/8157958/files/figure.png" pageId="6" pageNumber="179">Figure 1</figureCitation>
). Morphologically,
<taxonomicName id="4C7284CEFFCFFFECFB09FC06FA486A15" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[1279,1452,1022,1048]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFB09FC06FA486A15" box="[1279,1452,1022,1048]" italics="true" pageId="6" pageNumber="179">T. guizhouensis</emphasis>
</taxonomicName>
is well distinguishable from
<taxonomicName id="4C7284CEFFCFFFECFE20FBDBFD436A30" box="[470,679,1058,1085]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="undetermined">
<emphasis id="B906235FFFCFFFECFE20FBDBFD986A31" box="[470,636,1058,1084]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
sp.
</taxonomicName>
by quite smaller conidia (18.923 × 89 µm vs. (26) 31.546 × 1423 µm) and shorter appendages ((4.7) 12.618.9 (21.5) µm vs. 3690µm). The teleomorph of
<taxonomicName id="4C7284CEFFCFFFECFB10FBBEFA766A6D" authorityName="L. L. Liu &amp; Z. Y. Liu" authorityYear="2023" box="[1254,1426,1094,1120]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="species" species="guizhouensis">
<emphasis id="B906235FFFCFFFECFB10FBBEFA766A6D" box="[1254,1426,1094,1120]" italics="true" pageId="6" pageNumber="179">T. guizhouensis</emphasis>
</taxonomicName>
is unknown, however based on anamorph
<taxonomicName id="4C7284CEFFCFFFECFDB0FB93FD486A89" box="[582,684,1130,1156]" form="and" pageId="6" pageNumber="179" rank="form">form and</taxonomicName>
molecular data, it clearly belongs to
<taxonomicName id="4C7284CEFFCFFFECFBBDFB93FB156A89" box="[1099,1265,1130,1156]" class="Dothideomycetes" family="Tetraplosphaeriaceae" genus="Triplosphaeria" kingdom="Fungi" order="Pleosporales" pageId="6" pageNumber="179" phylum="Ascomycota" rank="genus">
<emphasis id="B906235FFFCFFFECFBBDFB93FB156A89" box="[1099,1265,1130,1156]" italics="true" pageId="6" pageNumber="179">Triplosphaeria</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>