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<document id="D2835EF7B2286FA744A5D8DF109A1CD5" ID-CLB-Dataset="68522" ID-DOI="10.11646/zootaxa.5150.2.2" ID-GBIF-Dataset="7b337d6b-61b0-42f7-81f6-4dd0eb4e3ca1" ID-ISSN="1175-5326" ID-Zenodo-Dep="6621227" ID-ZooBank="F457A107-44E8-4DBC-B4E9-FE8633E26360" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1654625418212" checkinUser="plazi" docAuthor="Guéron, Rodrigo, Almeida, Alexandre Oliveira, Aguilar, Robert, Ogburn, Matthew B., Prakash, Sanjeevi &amp; Baeza, J. Antonio" docDate="2022" docId="03DF38488D34FFFBB5EAFB1BFD42FE35" docLanguage="en" docName="zootaxa.5150.2.2.pdf" docOrigin="Zootaxa 5150 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Lysmata vittata" docType="treatment" docVersion="6" lastPageNumber="201" masterDocId="FFE640308D38FFF6B57DFFE9FFE2FFBC" masterDocTitle="Delimiting species within the Lysmata vittata (Stimpson, 1860) (Decapoda: Lysmatidae) species complex in a world full of invaders" masterLastPageNumber="216" masterPageNumber="189" pageNumber="201" updateTime="1699339042235" updateUser="ExternalLinkService" zenodo-license-document="CLOSED">
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<mods:title id="15D9AEF6FCC0B81658A8BE472DD38ACE">Delimiting species within the Lysmata vittata (Stimpson, 1860) (Decapoda: Lysmatidae) species complex in a world full of invaders</mods:title>
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<mods:namePart id="4DC89F221FB0130798D0D325C3B34CED">Guéron, Rodrigo</mods:namePart>
<mods:affiliation id="57387A86653BF0B6D0C1BBDB968B12A4">Departament of Zoology, Center of Biosciences, Federal University of Pernambuco (UFPE). Avenida Professor Moraes Rêgo. 1235. 50670 - 901 Recife, Pernambuco, Brazil.</mods:affiliation>
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<mods:namePart id="F3D97142321696E4719E5FDB78876E83">Almeida, Alexandre Oliveira</mods:namePart>
<mods:affiliation id="AA0190019C9EEAAA2682E79417661E7A">Departament of Zoology, Center of Biosciences, Federal University of Pernambuco (UFPE). Avenida Professor Moraes Rêgo. 1235. 50670 - 901 Recife, Pernambuco, Brazil.</mods:affiliation>
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<mods:namePart id="189E248B1F8C9DDE536396CB6CB72C29">Aguilar, Robert</mods:namePart>
<mods:affiliation id="4206AD3ED7F53B7CCD8D1818E589FCCC">Smithsonian Environmental Research Center, 647 Contees Wharf Rd., Edgewater, MD 21037, USA</mods:affiliation>
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<mods:namePart id="DA0CCA61A95D50AC18FEAAAD7EC26A74">Ogburn, Matthew B.</mods:namePart>
<mods:affiliation id="A2CB72DB6429B6F94093FDA06E14C57A">Smithsonian Environmental Research Center, 647 Contees Wharf Rd., Edgewater, MD 21037, USA</mods:affiliation>
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<mods:namePart id="74278C50A86E8CA378DC6F4FB2EB8DC4">Prakash, Sanjeevi</mods:namePart>
<mods:affiliation id="D58388E5C00741DF29E6186DB3454B81">Centre of Climate Change Studies, Sathyabama Institute of Science and Technology, Chennai, India &amp; Sathyabama Marine Research Station, Rameswaram, India</mods:affiliation>
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<mods:namePart id="AF63FE7C2875118DC0E26FAE67A8E5A9">Baeza, J. Antonio</mods:namePart>
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<mods:affiliation id="2DA3478DFF33E3A083B6F0078488F105">Department of Biological Sciences, 132 Long Hall, Clemson University, Clemson, SC 29634, USA &amp; Smithsonian Marine Station at Fort Pierce, 701 Seaway Drive, Fort Pierce, FL 34949, USA &amp; Departamento de Biología Marina, Facultad de Ciencias del Mar, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile Corresponding author. rggueron @ gmail. com; https: // orcid. org / 0000 - 0003 - 1848 - 742 X</mods:affiliation>
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<treatment id="03DF38488D34FFFBB5EAFB1BFD42FE35" ID-DOI="http://doi.org/10.5281/zenodo.6621185" ID-GBIF-Taxon="195827635" ID-Zenodo-Dep="6621185" LSID="urn:lsid:plazi:treatment:03DF38488D34FFFBB5EAFB1BFD42FE35" httpUri="http://treatment.plazi.org/id/03DF38488D34FFFBB5EAFB1BFD42FE35" lastPageId="13" lastPageNumber="201" pageId="12" pageNumber="201">
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<paragraph id="8BC9895E8D34FFFAB5EAFB1BFD26FAB1" blockId="12.[151,1437,1266,2013]" box="[151,708,1266,1293]" pageId="12" pageNumber="201">
<heading id="D0813E328D34FFFAB5EAFB1BFD26FAB1" bold="true" box="[151,708,1266,1293]" fontSize="11" level="1" pageId="12" pageNumber="201" reason="1">
<emphasis id="B902554C8D34FFFAB5EAFB1BFD26FAB1" bold="true" box="[151,708,1266,1293]" pageId="12" pageNumber="201">
Untangling the
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<emphasis id="B902554C8D34FFFAB42DFB1AFDE2FAB1" bold="true" box="[336,512,1267,1293]" italics="true" pageId="12" pageNumber="201">Lysmata vittata</emphasis>
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species complex
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<paragraph id="8BC9895E8D34FFFAB5EAFAFEFA92FA7D" blockId="12.[151,1437,1266,2013]" pageId="12" pageNumber="201">
Due to the great morphological variability within the
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<emphasis id="B902554C8D34FFFAB787FAFEFC83FA8C" box="[762,865,1303,1328]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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complex, absence of
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material, and history of doubtful records of this species, we applied an integrative analysis to delimit the putative species within the complex and resolve the taxonomic status of
<taxonomicName id="4C76F2DD8D34FFFAB718FAB6FD5BFAC5" authorityName="Laubenheimer &amp; Rhyne" authorityYear="2010" box="[613,697,1375,1401]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="rauli">
<emphasis id="B902554C8D34FFFAB718FAB6FD5BFAC5" box="[613,697,1375,1401]" italics="true" pageId="12" pageNumber="201">L. rauli</emphasis>
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and
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<emphasis id="B902554C8D34FFFAB78CFAB6FC7AFAC5" box="[753,920,1375,1401]" italics="true" pageId="12" pageNumber="201">L. durbanensis</emphasis>
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. Our integrated morphological and molecular data strongly support the hypothesis that
<taxonomicName id="4C76F2DD8D34FFFAB722FA6AFD26FA20" baseAuthorityName="Stimpson" baseAuthorityYear="1860" box="[607,708,1411,1436]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" sensu="lato" species="vittata">
<emphasis id="B902554C8D34FFFAB722FA6AFD26FA20" box="[607,708,1411,1436]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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is comprised of six taxonomic entities (including three undescribed species) with important morphological and distributional differences among constituent members.
</paragraph>
<paragraph id="8BC9895E8D34FFFAB5BAFA22FBA0F925" blockId="12.[151,1437,1266,2013]" pageId="12" pageNumber="201">
We noted clear and consistent morphological and color pattern differences among various members of the
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<emphasis id="B902554C8D34FFFAB0FBFA22FF3DF9B4" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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complex that were further supported by molecular analyses. Most strikingly was the absence of both an accessory branch (in
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<emphasis id="B902554C8D34FFFAB412F9FAFE37F990" box="[367,469,1555,1580]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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[clade LV1] and
<taxonomicName id="4C76F2DD8D34FFFAB7E1F9FAFCA6F991" authorityName="Stebbing" authorityYear="1921" box="[668,836,1555,1581]" class="Malacostraca" family="Hippolytidae" genus="Hippolysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="durbanensis">
<emphasis id="B902554C8D34FFFAB7E1F9FAFCA6F991" box="[668,836,1555,1581]" italics="true" pageId="12" pageNumber="201">L. durbanensis</emphasis>
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) and pleonal red transverse bands in
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<emphasis id="B902554C8D34FFFAB190F9FAFAB6F990" box="[1261,1364,1555,1580]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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, compared with a one-segmented accessory branch observed in
<taxonomicName id="4C76F2DD8D34FFFAB65CF9DEFC96F9ED" authorityName="Laubenheimer &amp; Rhyne" authorityYear="2010" box="[801,884,1591,1617]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="rauli">
<emphasis id="B902554C8D34FFFAB65CF9DEFC96F9ED" box="[801,884,1591,1617]" italics="true" pageId="12" pageNumber="201">L. rauli</emphasis>
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(clade LV4) and
<emphasis id="B902554C8D34FFFAB149F9DEFBA9F9EC" box="[1076,1099,1591,1616]" italics="true" pageId="12" pageNumber="201">L.</emphasis>
sp. AUS2 (clade LV3) and the presence of pleonal transverse bands in
<taxonomicName id="4C76F2DD8D34FFFAB732F9B2FD40F9C9" authorityName="Laubenheimer &amp; Rhyne" authorityYear="2010" box="[591,674,1627,1653]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="rauli">
<emphasis id="B902554C8D34FFFAB732F9B2FD40F9C9" box="[591,674,1627,1653]" italics="true" pageId="12" pageNumber="201">L. rauli</emphasis>
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. We also observed important differences in the number of carpal and meral segments of the second pereopod, particularly between
<taxonomicName id="4C76F2DD8D34FFFAB631F996FC53F924" baseAuthorityName="Stimpson" baseAuthorityYear="1860" box="[844,945,1663,1688]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="vittata">
<emphasis id="B902554C8D34FFFAB631F996FC53F924" box="[844,945,1663,1688]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
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and
<taxonomicName id="4C76F2DD8D34FFFAB69AF996FBD9F925" authorityName="Laubenheimer &amp; Rhyne" authorityYear="2010" box="[999,1083,1663,1689]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="rauli">
<emphasis id="B902554C8D34FFFAB69AF996FBD9F925" box="[999,1083,1663,1689]" italics="true" pageId="12" pageNumber="201">L. rauli</emphasis>
</taxonomicName>
.
</paragraph>
<paragraph id="8BC9895E8D34FFFAB5BAF94AFDF0F805" blockId="12.[151,1437,1266,2013]" pageId="12" pageNumber="201">
<materialsCitation id="3B1E83038D34FFFAB5BAF94AFDFFF95D" ID-GBIF-Occurrence="3804716301" collectionCode="ASAP" location="Further" pageId="12" pageNumber="201" specimenCount="1">
<location id="8EA9DF858D34FFFAB5BAF94AFEF9F901" LSID="urn:lsid:plazi:treatment:03DF38488D34FFFBB5EAFB1BFD42FE35:8EA9DF858D34FFFAB5BAF94AFEF9F901" box="[199,283,1699,1725]" name="Further" pageId="12" pageNumber="201">Further</location>
, our phylogeny recovered five strongly supported and divergent clades, (fig. 4), which were also delineated as putative species by
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<materialsCitation id="3B1E83038D34FFFAB759F92EFDECF805" ID-GBIF-Occurrence="3804716303" collectionCode="ABGD" country="United States of America" county="Automatic Barcode Gap Discovery" location="Taiwan" municipality="These" pageId="12" pageNumber="201" specimenCount="2">
and included LV1 (the eastern
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, Hong Kong [
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locality],
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, and
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), LV2 (a single individual from northern Australia), LV3 (northern
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), LV4 (Panama,
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[
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locality], Thailand, and
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), and LV5 (a single individual identified as “
<taxonomicName id="4C76F2DD8D34FFFAB1FDF8E6FB0AF894" baseAuthorityName="Stimpson" baseAuthorityYear="1860" box="[1152,1256,1807,1832]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="vittata">
<emphasis id="B902554C8D34FFFAB1FDF8E6FB0AF894" box="[1152,1256,1807,1832]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
</taxonomicName>
” from
<location id="8EA9DF858D34FFFAB040F8E6FA7BF895" LSID="urn:lsid:plazi:treatment:03DF38488D34FFFBB5EAFB1BFD42FE35:8EA9DF858D34FFFAB040F8E6FA7BF895" box="[1341,1433,1807,1833]" country="United States of America" county="Automatic Barcode Gap Discovery" municipality="These" name="Xiamen" pageId="12" pageNumber="201">Xiamen</location>
, China).
<location id="8EA9DF858D34FFFAB58DF8DAFEA5F8F1" LSID="urn:lsid:plazi:treatment:03DF38488D34FFFBB5EAFB1BFD42FE35:8EA9DF858D34FFFAB58DF8DAFEA5F8F1" box="[240,327,1843,1869]" country="United States of America" county="Automatic Barcode Gap Discovery" municipality="These" name="Genetic" pageId="12" pageNumber="201">Genetic</location>
differences were extremely wide, with minimum inter-clade genetic distances (
<emphasis id="B902554C8D34FFFAB1C4F8DAFB25F8F0" box="[1209,1223,1843,1868]" italics="true" pageId="12" pageNumber="201">p</emphasis>
-distances) ranging from 0.048 (LV3 vs. LV4) to 0.430 (LV2 vs. LV5).
<collectingMunicipality id="6BAD13248D34FFFAB7ADF8BEFCF1F8CD" box="[720,787,1879,1905]" pageId="12" pageNumber="201">These</collectingMunicipality>
findings are bolstered by
<bibRefCitation id="EFE7F4AF8D34FFFAB14FF8BEFAFBF8CD" author="Aguilar, R. &amp; Prakash, S. &amp; Ogburn, M. B. &amp; Lohan, K. M. P. &amp; MacDonald III, K. S. &amp; Driskell, A. C. &amp; Ahyong, S. T. &amp; Leray, M. &amp; McIlroy, S. E. &amp; Tuckey, T. D. &amp; Baeza, J. A." box="[1074,1305,1879,1905]" pageId="12" pageNumber="201" pagination="1" refId="ref16244" refString="Aguilar, R., Prakash, S., Ogburn, M. B., Lohan, K. M. P., MacDonald III, K. S., Driskell, A. C., Ahyong, S. T., Leray, M., McIlroy, S. E., Tuckey, T. D. &amp; Baeza, J. A. (2022) Unresolved taxonomy can confounds invasive species identification: the Lysmata vittata (Stimpson, 1860) (Decapoda: Caridea: Lysmatidae) species complex and recent introduction of Lysmata vittata sensu stricto in the western Atlantic. Journal of Crustacean Biology, 42 (1). https: // doi. org / 10.1093 / jcbiol / ruab 079" type="journal article" year="2022">
Aguilar
<emphasis id="B902554C8D34FFFAB1F3F8BEFB24F8CD" box="[1166,1222,1879,1905]" italics="true" pageId="12" pageNumber="201">et al.</emphasis>
(2022)
</bibRefCitation>
, which also recovered four similar strongly supported clades and were all delimited as putative species using
<collectingCounty id="62A8F1D28D34FFFAB1BEF892FEA2F805" pageId="12" pageNumber="201">Automatic Barcode Gap Discovery</collectingCounty>
analysis (
<collectionCode id="ED67119B8D34FFFAB4CDF876FDE4F805" box="[432,518,1951,1977]" pageId="12" pageNumber="201">ABGD</collectionCode>
)
</materialsCitation>
.
</paragraph>
<paragraph id="8BC9895E8D34FFFBB5BAF82AFD42FE35" blockId="12.[151,1437,1266,2013]" lastBlockId="13.[151,1436,151,393]" lastPageId="13" lastPageNumber="202" pageId="12" pageNumber="201">
The fixing of a
<typeStatus id="54CD37FC8D34FFFAB408F82AFE2CF861" box="[373,462,1987,2013]" pageId="12" pageNumber="201" type="neotype">neotype</typeStatus>
by
<bibRefCitation id="EFE7F4AF8D34FFFAB488F82AFD3CF861" author="Aguilar, R. &amp; Prakash, S. &amp; Ogburn, M. B. &amp; Lohan, K. M. P. &amp; MacDonald III, K. S. &amp; Driskell, A. C. &amp; Ahyong, S. T. &amp; Leray, M. &amp; McIlroy, S. E. &amp; Tuckey, T. D. &amp; Baeza, J. A." box="[501,734,1987,2013]" pageId="12" pageNumber="201" pagination="1" refId="ref16244" refString="Aguilar, R., Prakash, S., Ogburn, M. B., Lohan, K. M. P., MacDonald III, K. S., Driskell, A. C., Ahyong, S. T., Leray, M., McIlroy, S. E., Tuckey, T. D. &amp; Baeza, J. A. (2022) Unresolved taxonomy can confounds invasive species identification: the Lysmata vittata (Stimpson, 1860) (Decapoda: Caridea: Lysmatidae) species complex and recent introduction of Lysmata vittata sensu stricto in the western Atlantic. Journal of Crustacean Biology, 42 (1). https: // doi. org / 10.1093 / jcbiol / ruab 079" type="journal article" year="2022">
Aguilar
<emphasis id="B902554C8D34FFFAB72FF82AFD68F861" box="[594,650,1987,2013]" italics="true" pageId="12" pageNumber="201">et al.</emphasis>
(2022)
</bibRefCitation>
, provided the necessary criteria to define
<taxonomicName id="4C76F2DD8D34FFFAB1CDF82AFAF7F860" baseAuthorityName="Stimpson" baseAuthorityYear="1860" box="[1200,1301,1987,2012]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="12" pageNumber="201" phylum="Arthropoda" rank="species" species="vittata">
<emphasis id="B902554C8D34FFFAB1CDF82AFAF7F860" box="[1200,1301,1987,2012]" italics="true" pageId="12" pageNumber="201">L. vittata</emphasis>
</taxonomicName>
, with major morphological characters including: 1) uniramous dorsal antennule; 2) rostrum reaching distal end of second antennular article; 3) scaphocerite overreaching the end of the antennular peduncle; 4) stylocerite reaching the mid-length of the first antennular article; 5) presence of a pterygostomial spine; 6) 813 meral segments of the second pereopod; 7) 1823 carpal segments of the second pereopod; and 8) coloration pattern consisting of numerous longitudinal red stripes without dark transverse bands on the pleon. These important morphological criteria provided a framework to delimit the putative species within the
<taxonomicName id="4C76F2DD8D35FFFBB731FEA2FD51FED8" baseAuthorityName="Stimpson" baseAuthorityYear="1860" box="[588,691,331,356]" class="Malacostraca" family="Hippolytidae" genus="Lysmata" higherTaxonomySource="GBIF" kingdom="Animalia" order="Decapoda" pageId="13" pageNumber="202" phylum="Arthropoda" rank="species" species="vittata">
<emphasis id="B902554C8D35FFFBB731FEA2FD51FED8" box="[588,691,331,356]" italics="true" pageId="13" pageNumber="202">L. vittata</emphasis>
</taxonomicName>
species complex using integrative taxonomic methods, helping to settle over a century of taxonomic uncertainty.
</paragraph>
</subSubSection>
</treatment>
</document>