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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.102.113696" ID-Pensoft-Pub="1314-4049-102-201" ID-Pensoft-UUID="86674352E59B513ABE2F03A64B2F2AEB" ModsDocID="1314-4049-102-201" checkinTime="1709112337990" checkinUser="pensoft" docAuthor="Wang, Yifeng, Tsui, Kin-Ming, Chen, Shimei &amp; You, Chongjuan" docDate="2024" docId="2D9F5655D13951B59AB8D120608C92C7" docLanguage="en" docName="MycoKeys 102: 201-224" docOrigin="MycoKeys 102" docPubDate="2024-02-27" docSource="http://dx.doi.org/10.3897/mycokeys.102.113696" docTitle="Pestalotiopsis trachycarpicola Yan M. Zhang &amp; K. D. Hyde 2012" docType="treatment" docVersion="1" id="86674352E59B513ABE2F03A64B2F2AEB" lastPageNumber="201" masterDocId="86674352E59B513ABE2F03A64B2F2AEB" masterDocTitle="Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China" masterLastPageNumber="224" masterPageNumber="201" pageNumber="201" updateTime="1709112337990" updateUser="pensoft">
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<mods:title id="9669CC5D188D0047F9224FC268534195">Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China</mods:title>
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<mods:namePart id="7ECF74E069BE8360AC9D2985F07B4717">Wang, Yifeng</mods:namePart>
<mods:nameIdentifier id="D75943A5A9CFD150E88A89F1988FD7B6" type="ORCID">https://orcid.org/0009-0006-6533-6615</mods:nameIdentifier>
<mods:affiliation id="4A15E35F120A3D3D0556D86513BD9350">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
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<mods:namePart id="655C50CF950E0FE80AEA162ACA3AD03A">Tsui, Kin-Ming</mods:namePart>
<mods:nameIdentifier id="204F040670D6C7ABF5C54B66DC75601E" type="ORCID">https://orcid.org/0000-0001-5129-1037</mods:nameIdentifier>
<mods:affiliation id="804E52FB6972BE5D5EF60FFE4A2305BC">National Centre for Infectious Diseases, Tan Tock Seng Hospital, Singapore 308433, Singapore &amp; Faculty of Medicine, University of British Columbia, Vancouver, V 6 T 1 Z 3, Canada</mods:affiliation>
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<mods:namePart id="2228C775A0610934E3EE8094664E47EC">Chen, Shimei</mods:namePart>
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<mods:affiliation id="BA493235B635D6038858FB31C6DD564B">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
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<mods:namePart id="88045AA35137EAF342C0867FA53EFCCC">You, Chongjuan</mods:namePart>
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<mods:affiliation id="FD99544F5838586C7B7490D400AD083C">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
<mods:nameIdentifier id="C33969D0C7A78B78292F14C760C45A02" type="email">chongjuanyou@bjfu.edu.cn</mods:nameIdentifier>
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<mods:date id="5F6AD92DB3286989F14B14EEDFF0F74D">2024</mods:date>
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<treatment id="2D9F5655D13951B59AB8D120608C92C7" LSID="urn:lsid:plazi:treatment:2D9F5655D13951B59AB8D120608C92C7" httpUri="http://treatment.plazi.org/id/2D9F5655D13951B59AB8D120608C92C7" lastPageNumber="201" pageId="0" pageNumber="201">
<subSubSection id="6C7CA7BD01A8A6F0F8B91AF4BA048DE6" pageId="0" pageNumber="201" type="nomenclature">
<paragraph id="211B892C5CAD4C22CAF489B28D92A05E" pageId="0" pageNumber="201">
<taxonomicName id="1BC38B274ACFEB8D0CB114D8D68F6CD2" ID-CoL="4FC95" LSID="2D9F5655-D139-51B5-9AB8-D120608C92C7" authority="Yan M. Zhang &amp; K. D. Hyde, 2012" authorityName="Yan M. Zhang &amp; K. D. Hyde" authorityYear="2012" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pestalotiopsis trachycarpicola" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="species" species="trachycarpicola">Pestalotiopsis trachycarpicola Yan M. Zhang &amp; K.D. Hyde, 2012</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection id="9C0578DEEE0B33133FCF3E6795CD628F" pageId="0" pageNumber="201" type="description">
<paragraph id="E6DA7344755D1383D944689E9EFEF6A6" pageId="0" pageNumber="201">
<figureCitation id="9BC50182679ECF9E807CAC142B3967C0" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Pestalotiopsis trachycarpicola (BJFUCC 42) a culture on PDA b conidiomata formed on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure4" httpUri="https://binary.pensoft.net/fig/993280" pageId="0" pageNumber="201">Fig. 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="B2340773AD58A4D9E3ACAECE4AF32B15" pageId="0" pageNumber="201" type="conidiogenesis">
<paragraph id="8F586FC3E0B9A781DA5EEA3CC76E2548" pageId="0" pageNumber="201">Conidiogenesis.</paragraph>
<paragraph id="13C92448850844B77B9BD7B9D0FD86AB" pageId="0" pageNumber="201">
Conidiophores reduced to conidiogenous cells, indistinct. Conidiogenous cells were discrete, ampulliform, thin-walled, hyaline, smooth. Conidia fusiform to clavate, straight or slightly curved, olivaceous to brown, 4-septate, 18.5-25
<normalizedToken id="9B2C06045739CD604F8593081F1B4A00" originalValue="×">x</normalizedToken>
4-6
<normalizedToken id="75601BAAA1462F78453FA8B2320387B7" originalValue="μm">μm</normalizedToken>
, with apical and basal appendages. Basal cell obconic, hyaline, thin-walled, smooth, 3-5
<normalizedToken id="9F91DE0F2C3760148C47863781931B28" originalValue="µm">µm</normalizedToken>
; the three median cells dolioform, versicolor, pale brown to brown with septa darker than the rest of the cells, 11.5-13.5
<normalizedToken id="CB2CA4ECEECC0E76EA10C92F3C1EC679" originalValue="µm">µm</normalizedToken>
, the second cell from base 3.5-6
<normalizedToken id="9924E5DE2DDC0D08AAA8757ABB22E174" originalValue="µm">µm</normalizedToken>
; the third cell 3.5-4.5
<normalizedToken id="65EE371E98C148FAAE1A3BA195E05E0C" originalValue="µm">µm</normalizedToken>
; the fourth cell 3.5-5
<normalizedToken id="50230A1E054D86F778F05557F3E8CDE7" originalValue="µm">µm</normalizedToken>
; apical cell 2.5-4
<normalizedToken id="80B87F23189D16088BD71FA0BEE4518F" originalValue="µm">µm</normalizedToken>
, cylindrical, hyaline; 2-4 tubular apical appendages, arising from the apex of the apical cell each at different point, filiform, 5-15
<normalizedToken id="E80C0A21D02FF2FAF6C425A21D58F757" originalValue="µm">µm</normalizedToken>
; basal appendage present most of the time, single, tubular, unbranched, 3.5-4.5
<normalizedToken id="1729A0610D1807E89429023772281521" originalValue="µm">µm</normalizedToken>
(Fig.
<figureCitation id="DBB29CB8460C8A4D396391A70F3ACED5" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Pestalotiopsis trachycarpicola (BJFUCC 42) a culture on PDA b conidiomata formed on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure4" httpUri="https://binary.pensoft.net/fig/993280" pageId="0" pageNumber="201">4c-e</figureCitation>
). Sexual morph not observed.
</paragraph>
<caption id="430A7FB4111D8A9C2FA29279AA9F66FF" doi="10.3897/mycokeys.102.113696.figure4" httpUri="https://binary.pensoft.net/fig/993280" pageId="0" pageNumber="201" start="Figure 4" startId="F4">
<paragraph id="DD7A84B05F4A811F01AFE9CF0B52A252" pageId="0" pageNumber="201">
<emphasis id="D0DD63753715DBD86135AD1B432DCD14" bold="true" pageId="0" pageNumber="201">Figure 4.</emphasis>
<taxonomicName id="BECF33C089C773337BA11646557B2817" authorityName="Yan M. Zhang &amp; K. D. Hyde" authorityYear="2012" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pestalotiopsis trachycarpicola" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="species" species="trachycarpicola">
<emphasis id="A24C3FDE417661BBBDF6C4DCA6077012" italics="true" pageId="0" pageNumber="201">Pestalotiopsis trachycarpicola</emphasis>
</taxonomicName>
(BJFUCC42)
<emphasis id="03D2D490085F24969E944F159CD9C025" bold="true" pageId="0" pageNumber="201">a</emphasis>
culture on PDA
<emphasis id="C206A062F442F286FA9EF5B7AF40F2B3" bold="true" pageId="0" pageNumber="201">b</emphasis>
conidiomata formed on PDA
<emphasis id="8D9E2B1AE20B4D85F9EDAFB5682F12DE" bold="true" pageId="0" pageNumber="201">c-e</emphasis>
conidia. Scale bars: 500
<normalizedToken id="98FDCDC5F94B4CC1990959BCF7119587" originalValue="µm">µm</normalizedToken>
(
<emphasis id="B9FC5A036797E38B8FD39C0B6CECEC21" bold="true" pageId="0" pageNumber="201">b</emphasis>
); 10
<normalizedToken id="33715FA15D3D8EE32F0130B6EA74061D" originalValue="µm">µm</normalizedToken>
(
<emphasis id="E87E4F239B9AB154FC25F3EE99A060D8" bold="true" pageId="0" pageNumber="201">c-e</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="F8426D04E12D390EFA65C504D1DAF5B8" pageId="0" pageNumber="201" type="description">
<paragraph id="EF61CA6B77C00AF1957437C76976A638" pageId="0" pageNumber="201">Culture characteristics.</paragraph>
<paragraph id="33CAB5471DFDD910E7176C069A8636D8" pageId="0" pageNumber="201">
Colonies on PDA reaching 90 mm diameter after seven days at 25 °C, with an undulate and radial edge, with dense aerial mycelium on surface, white to faint yellow on front, pale honey-coloured on the reverse side (Fig.
<figureCitation id="3E38C36CA4556108E2E488A8AF251F8B" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Pestalotiopsis trachycarpicola (BJFUCC 42) a culture on PDA b conidiomata formed on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure4" httpUri="https://binary.pensoft.net/fig/993280" pageId="0" pageNumber="201">4a</figureCitation>
).
<emphasis id="0DE976D262366F62EC665714C04B75B4" italics="true" pageId="0" pageNumber="201">Conidiomata acervular</emphasis>
in culture on PDA, globose, 100-500
<normalizedToken id="C4984A90C25B8F249D950F46331471CC" originalValue="μm">μm</normalizedToken>
in diameter, solitary or aggregated in clusters, exuding black conidial masses (Fig.
<figureCitation id="0816AA791F4121BE6091A5F62D10F27D" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Pestalotiopsis trachycarpicola (BJFUCC 42) a culture on PDA b conidiomata formed on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure4" httpUri="https://binary.pensoft.net/fig/993280" pageId="0" pageNumber="201">4b</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="9A52D42427DD562D6ACFBA3F37F74F73" pageId="0" pageNumber="201" type="materials_examined">
<paragraph id="EB4BA68CF312E744763848928A2AB8AC" pageId="0" pageNumber="201">Material examined.</paragraph>
<paragraph id="2198C6BFF590EDCF4BD9CC82B6BF00EB" pageId="0" pageNumber="201">
<materialsCitation id="EB7401E9F98347FADD50778C4856F0A2" collectingDate="2020-05" collectorName="Y. F. Wang" country="China" location="Chinese" specimenCount="1" stateProvince="Guangxi Province">
<collectingCountry id="31760A7855C140789C716EBFC7123A01" name="China">China</collectingCountry>
,
<collectingRegion id="4405FD92602AE7B126E5516E04FC4939" country="China" name="Guangxi">Guangxi Province</collectingRegion>
, from diseased needles of
<location id="B51C3D184B536D9DBEBFBD0167E6C83B" LSID="urn:lsid:plazi:treatment:2D9F5655D13951B59AB8D120608C92C7:B51C3D184B536D9DBEBFBD0167E6C83B" country="China" name="Chinese" stateProvince="Guangxi Province">Chinese</location>
yew,
<collectingDate id="5C9A26835A78A4A78CE16B9608D79226" value="2020-05">May 2020</collectingDate>
,
<collectorName id="FF6C61059A3BBE72436433F7034DABA9">Y. F. Wang</collectorName>
(BJFC-S1955); living cultures BJFUCC42, BJFUCC42-2
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="4C8A16C9FC2FC23A76FE96523A7B9983" pageId="0" pageNumber="201" type="notes">
<paragraph id="C7348EE4AE70E106D8E99318DE980CE8" pageId="0" pageNumber="201">Notes.</paragraph>
<paragraph id="0212BDB9A7B226EB6D83A673D78FE034" pageId="0" pageNumber="201">
<taxonomicName id="57D76B365CE6DA564D373BF7A9C23AC4" authorityName="Yan M. Zhang &amp; K. D. Hyde" authorityYear="2012" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Pestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pestalotiopsis trachycarpicola" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="species" species="trachycarpicola">
<emphasis id="FC10CF8AEC2C127722CD684272F24D11" italics="true" pageId="0" pageNumber="201">Pestalotiopsis trachycarpicola</emphasis>
</taxonomicName>
was originally described from leaves of
<taxonomicName id="7091B643500289FD16D09DF74F1F935E" class="Sordariomycetes" family="Sporocadaceae" genus="Trachycarpus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Trachycarpus fortunei" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="species" species="fortunei">
<emphasis id="293078CF19042C6A803A014E558C7A06" italics="true" pageId="0" pageNumber="201">Trachycarpus fortunei</emphasis>
</taxonomicName>
in Kunming Botany Garden, Kunming, Yunnan Province, China (
<bibRefCitation id="71DD770F4957FBBB5F139150DC89398D" DOI="https://doi.org/10.7872/crym.v33.iss3.2012.311" author="Zhang, Y" journalOrPublisher="Mycologie" pageId="0" pageNumber="201" pagination="311 - 318" refId="B52" refString="Zhang, Y, Maharachchikumbura, SS, Mckenzie, EH, Hyde, KD, 2012. A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie. Mycologie 33 (3): 311 - 318, DOI: https://doi.org/10.7872/crym.v33.iss3.2012.311" title="A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie." url="https://doi.org/10.7872/crym.v33.iss3.2012.311" volume="33" year="2012">Zhang et al. 2012</bibRefCitation>
). In the present study, the two isolates clustered with
<taxonomicName id="43767D132521234AEDA750A4B6105AF8" lsidName="P. trachycarpicola" pageId="0" pageNumber="201" rank="species" species="trachycarpicola">
<emphasis id="8F00485F22DACC71BDC4914A5F9E2308" italics="true" pageId="0" pageNumber="201">P. trachycarpicola</emphasis>
</taxonomicName>
and
<taxonomicName id="1FC13164226EE38D2C4E478A63717157" lsidName="P. kenyana" pageId="0" pageNumber="201" rank="species" species="kenyana">
<emphasis id="644AB0334909D6A3EAC9EFB00EBFD60E" italics="true" pageId="0" pageNumber="201">P. kenyana</emphasis>
</taxonomicName>
with high support values (MP/ML = 86/100).
<taxonomicName id="C3F96A6177B1968D45BB36B48822C18A" lsidName="P. kenyana" pageId="0" pageNumber="201" rank="species" species="kenyana">
<emphasis id="E2ADA3E82CEFAA8F9EA08BA0A517344B" italics="true" pageId="0" pageNumber="201">P. kenyana</emphasis>
</taxonomicName>
(CBS 442.67, ex-type) and
<taxonomicName id="6A6D0DA7D2197EF7ACF0BE5CAC6F2DFC" lsidName="P. trachicarpicola" pageId="0" pageNumber="201" rank="species" species="trachicarpicola">
<emphasis id="C6AA5778692D5F92DC6861318FF49523" italics="true" pageId="0" pageNumber="201">P. trachicarpicola</emphasis>
</taxonomicName>
(OP068, ex-type MFLUCC 12-0263) were demonstrated to be the same species, as there was no genetic distance between the two samples (Suppl. material 3). Morphologically, our new collections resembled
<taxonomicName id="679B9AC3D5A6ECD1AF1B1248120A17A0" lsidName="P. trachycarpicola" pageId="0" pageNumber="201" rank="species" species="trachycarpicola">
<emphasis id="A138BFEEEB309FC8E4F28404E9FDD87C" italics="true" pageId="0" pageNumber="201">P. trachycarpicola</emphasis>
</taxonomicName>
in colour and size of the conidiogenous cells, conidia and appendages (
<bibRefCitation id="E2FD43B97EABDA65830D4A2CFCD324BB" DOI="https://doi.org/10.7872/crym.v33.iss3.2012.311" author="Zhang, Y" journalOrPublisher="Mycologie" pageId="0" pageNumber="201" pagination="311 - 318" refId="B52" refString="Zhang, Y, Maharachchikumbura, SS, Mckenzie, EH, Hyde, KD, 2012. A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie. Mycologie 33 (3): 311 - 318, DOI: https://doi.org/10.7872/crym.v33.iss3.2012.311" title="A novel species of Pestalotiopsis causing leaf spots of Trachycarpus fortunei. Cryptogamie." url="https://doi.org/10.7872/crym.v33.iss3.2012.311" volume="33" year="2012">Zhang et al. 2012</bibRefCitation>
). Therefore, we reported the two isolates as a new host record of
<taxonomicName id="A3FADB2A757B5BC80B3EDFB4934954E7" lsidName="P. trachycarpicola" pageId="0" pageNumber="201" rank="species" species="trachycarpicola">
<emphasis id="A99FDABCD6E8A118382AD7F207F6212F" italics="true" pageId="0" pageNumber="201">P. trachycarpicola</emphasis>
</taxonomicName>
from yews.
</paragraph>
</subSubSection>
</treatment>
</document>