396 lines
43 KiB
XML
396 lines
43 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.99.90117" ID-Pensoft-Pub="1314-4049-99-45" ID-Pensoft-UUID="47D9DA3E1C56567CA14356476272F738" ModsDocID="1314-4049-99-45" checkinTime="1692098463846" checkinUser="pensoft" docAuthor="Itagaki, Hiyori & Hosoya, Tsuyoshi" docDate="2023" docId="77BC3F0E25D95CD3974C13953D4647A7" docLanguage="en" docName="MycoKeys 99: 45-85" docOrigin="MycoKeys 99" docPubDate="2023-08-14" docSource="http://dx.doi.org/10.3897/mycokeys.99.90117" docTitle="Trichobelonium miscanthi Itagaki & Hosoya 2023, sp. nov." docType="treatment" docVersion="1" id="47D9DA3E1C56567CA14356476272F738" lastPageNumber="45" masterDocId="47D9DA3E1C56567CA14356476272F738" masterDocTitle="A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan" masterLastPageNumber="85" masterPageNumber="45" pageNumber="45" updateTime="1692098463846" updateUser="pensoft">
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<mods:title id="148D3F7D2B1BBBC0CA3AF06BBB2D7C81">A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan</mods:title>
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<mods:name id="0FFF3EF0099D4AFAE32261EE434E5CA4" type="personal">
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<mods:role id="C588718121C89A9F138AE3C0630F94B8">
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<mods:roleTerm id="EA8B72588CA72CA1B9D67B48FB1F492D">Author</mods:roleTerm>
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<mods:namePart id="4BA8CF8F8F455C6CF972D0CEF510F655">Itagaki, Hiyori</mods:namePart>
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<mods:nameIdentifier id="BA45444B76DACBE9D77F3B6045BCC710" type="ORCID">https://orcid.org/0000-0001-8678-0826</mods:nameIdentifier>
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<mods:affiliation id="C04450A4CE35D1F7C27B834512BF07BC">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<mods:nameIdentifier id="55FE88159D1FDFB03EF12763A6EE5764" type="email">itagaki@kahaku.go.jp</mods:nameIdentifier>
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</mods:name>
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<mods:name id="94A170B4EAD7552DD599476DAE05B074" type="personal">
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<mods:roleTerm id="FDCAC6DD64199E828F96B918C0ADD61C">Author</mods:roleTerm>
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</mods:role>
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<mods:namePart id="493423E208DB2E69792683F0CCD4FE14">Hosoya, Tsuyoshi</mods:namePart>
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<mods:nameIdentifier id="4B12DB7991EF105189617BAA39763A91" type="ORCID">https://orcid.org/0000-0001-5360-5677</mods:nameIdentifier>
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<mods:affiliation id="5B1B9DDA8EF96D17F2D0A0174745B56E">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<mods:typeOfResource id="1832DAFC89E6DD395041483B87A0704A">text</mods:typeOfResource>
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<mods:titleInfo id="8C1402A89B04AE5CD0EBFEC56E03D9E3">
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<mods:title id="AA09E9CACDB438B656DE8F324E8E0F07">MycoKeys</mods:title>
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<mods:part id="1535E419B9E9AEA5F680A76D06AAE9E0">
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<mods:date id="E311525BA186CF33A875DC50891E5CBA">2023</mods:date>
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<mods:detail id="54BEDC9E76331FF43E6990DA1FED17A6" type="pubDate">
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<mods:number id="54C54A9916F9F6653A8B1FAAE530D34F">2023-08-14</mods:number>
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<mods:number id="39DCD7AEA04F4D70C92393315A236558">99</mods:number>
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<mods:start id="4672E174D65AA143B3AB793DFAA2A2C0">45</mods:start>
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<mods:end id="230FAF33F51DCCF9AAC1458C816DE48F">85</mods:end>
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<mods:location id="0437C790FF34740435DBAF13E9EBCD58">
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<mods:url id="E775BF60A68D1787ED300D8386C619DF">http://dx.doi.org/10.3897/mycokeys.99.90117</mods:url>
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<mods:classification id="D1BFC4F0E6B16CE8D1F61EF87B266918">journal article</mods:classification>
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<mods:identifier id="017F6C76BBA94969032480F788462AE6" type="DOI">http://dx.doi.org/10.3897/mycokeys.99.90117</mods:identifier>
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<mods:identifier id="95F35E93019AB5840D6E1ED703B29D17" type="Pensoft-Pub">1314-4049-99-45</mods:identifier>
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</mods:mods>
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<subSection id="488FFC25C2B7449B8B89E59ECF74DA96" pageId="0" pageNumber="45" type="results">
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<treatment id="77BC3F0E25D95CD3974C13953D4647A7" LSID="urn:lsid:plazi:treatment:77BC3F0E25D95CD3974C13953D4647A7" httpUri="http://treatment.plazi.org/id/77BC3F0E25D95CD3974C13953D4647A7" lastPageNumber="45" pageId="0" pageNumber="45">
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<subSubSection id="296E37067788CA7342326687B21485AD" pageId="0" pageNumber="45" type="nomenclature">
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<paragraph id="B49E5213B58CC09D7BEEAF6B2CD66753" pageId="0" pageNumber="45">
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<taxonomicName id="F3A0106DBD246CC2A1DF54F3264FEF63" LSID="77BC3F0E-25D9-5CD3-974C-13953D4647A7" authority="Itagaki & Hosoya" authorityName="Itagaki & Hosoya" authorityYear="2023" class="Leotiomycetes" family="Mollisiaceae" genus="Trichobelonium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobelonium miscanthi" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="miscanthi" status="sp. nov.">Trichobelonium miscanthi Itagaki & Hosoya</taxonomicName>
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<taxonomicNameLabel id="7DC76D4D2AE97DE0582CD485BC524001" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection id="627CFD09E04A925D59A13A72CB60CDFD" pageId="0" pageNumber="45" type="description">
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<paragraph id="DF8AC0D92E8B1910ABE491161E2492CC" pageId="0" pageNumber="45">
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<figureCitation id="3E1B5C8FC2208AC03EC03AABBE671F76" captionStart="Figure 12" captionStartId="F12" captionText="Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." figureDoi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45">Figs 12</figureCitation>
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<figureCitation id="E0623AE9E6E61FD0124F8B59C6BCB7BB" captionStart="Figure 13" captionStartId="F13" captionText="Figure 13. Line-drawings of the Neobelonopsis and Trichobelonium species I vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium II asci III paraphyses IV ascospores. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure13" httpUri="https://binary.pensoft.net/fig/892429" pageId="0" pageNumber="45">, 13</figureCitation>
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<figureCitation id="81752971FB0124564E5FE0A647D31BE6" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">, 14I</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection id="8CFDF4EA90552420ACC28991438EA334" pageId="0" pageNumber="45" type="etymology">
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<paragraph id="1C4F035154C1B86A79959C898FF1871D" pageId="0" pageNumber="45">Etymology.</paragraph>
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<paragraph id="36E65039F41BD6AA626BC24403018DC7" pageId="0" pageNumber="45">
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Named after the genus of its host,
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<taxonomicName id="DE7DE4E756B3A4AE8286B6D52E079281" class="Ascomycetes" family="Hyaloscyphaceae" genus="Miscanthus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Miscanthus" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="genus">
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<emphasis id="C8C034F69C9743FED8BB34A9D7D1A2AA" italics="true" pageId="0" pageNumber="45">Miscanthus</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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<subSubSection id="ACADF5D122A751F344B6A631F4769F2B" pageId="0" pageNumber="45" type="diagnosis">
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<paragraph id="89A7991144F70B3672A5F78CEA5AE550" pageId="0" pageNumber="45">Diagnosis.</paragraph>
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<paragraph id="6874BA058800CA01C2AD8AD254B1CF30" pageId="0" pageNumber="45">Characterized by 5-septate ascospores and sparse subiculum</paragraph>
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</subSubSection>
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<subSubSection id="498A3F81F4C5CF67731259DCEB63193A" pageId="0" pageNumber="45" type="holotype">
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<paragraph id="A437FC902323EEE9E1D0E27A7D0F8956" pageId="0" pageNumber="45">Holotype.</paragraph>
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<paragraph id="EAC0C69167299D08C84ECC3947DFA9CD" pageId="0" pageNumber="45">
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TNS-F-17835, Sugadaira Montane Research Center, Ueda City, Nagano Pref., 17 September 2005, on decaying culm of
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<taxonomicName id="BB38442602031C2BF254B7EC23FCD462" class="Ascomycetes" family="Hyaloscyphaceae" genus="Miscanthus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Miscanthus sinensis" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sinensis">
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<emphasis id="1BD097C94C09F2A835E3149977CEBD4D" italics="true" pageId="0" pageNumber="45">Miscanthus sinensis</emphasis>
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</taxonomicName>
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, ex-holotype culture NBRC 115566.
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</paragraph>
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</subSubSection>
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<subSubSection id="0F07D9DA0F297879DAF1DBFC337C7600" pageId="0" pageNumber="45" type="description">
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<paragraph id="E80112D97894E0AEE9A50B3D758AB421" pageId="0" pageNumber="45">Description.</paragraph>
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<paragraph id="FF0BFBA255E76A8FC5967C48096D722C" pageId="0" pageNumber="45">
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<emphasis id="DEE2FA6EEDA4E9CC221F918D3E19D084" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
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developed from scuta.
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<emphasis id="5EA8ACE6EBBC2A8859797A4FA9E5FA4F" bold="true" italics="true" pageId="0" pageNumber="45">Scuta</emphasis>
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superficial, scattered to gregarious, flat discoid, 145-180
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<normalizedToken id="3C2C1A65E2FBC3A3D2E004A47AACAFF8" originalValue="µm">µm</normalizedToken>
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diam., dark brown (C80M100Y80K60),
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<emphasis id="93832B1EF1E1546D080BA81541F28451" bold="true" italics="true" pageId="0" pageNumber="45">textura epidermoidea</emphasis>
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, gradually becoming
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<emphasis id="A14B4085C2D4290682BA8F151A11B071" bold="true" italics="true" pageId="0" pageNumber="45">textura porrecta</emphasis>
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and connecting to subiculum.
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<emphasis id="FC30A7ABAB1BFCF7241FD33E528D3C55" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
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sessile, globose to pulvinate when immature, discoid to saucer-shape when mature, flat to slightly convex when fresh, 0.2 mm high, with brown (C30-60M60Y80-100K60) receptacle; disc 0.5-1.5 mm diam., entire to slightly undulate, without hairs at margin, waxy, white to pale yellow (Y10-30) when fresh, shrunk to 0.3-1 mm diam., pulverulent, cream (Y20-40K10) when dried. Ectal excipulum 25-35
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<normalizedToken id="5DCFDB8B097DD0B4D1E5786C5F86F2AC" originalValue="µm">µm</normalizedToken>
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thick at base, approximately 25
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<normalizedToken id="C6E16DA53889A9DBE6604FA00EB1F66D" originalValue="µm">µm</normalizedToken>
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thick at the upper flank to margin,
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<emphasis id="8EB1ACC39FED6C225338CF7D9CFB9C55" bold="true" italics="true" pageId="0" pageNumber="45">textura globulosa</emphasis>
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and
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<emphasis id="85D4B3153808ABEBD099BB38D52A21CA" bold="true" italics="true" pageId="0" pageNumber="45">angularis</emphasis>
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, composed of 2-4 layers of brown thick-walled cells, not gelatinized, without crystals or exudates; cortical cells in middle to lower flank pyriform to clavate, with protruded cells, 16-21(-23)
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<normalizedToken id="7468A9969E8D626119337941415122A1" originalValue="×">x</normalizedToken>
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5-7
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<normalizedToken id="4F67CBF21E7279C8CD609A8782570111" originalValue="µm">µm</normalizedToken>
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, containing with refractive vacuoles at margin when mounted fresh in water; anchoring hyphae connecting the cortical calls of the flank and subiculum, radially extending from apothecium, 2.5-3
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<normalizedToken id="355F8B2C8702A99ADA40077BB3A7674F" originalValue="µm">µm</normalizedToken>
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width, thin-walled, brown. Medullary excipulum 40-75
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<normalizedToken id="86398627736A03F2C25CE0B1D00DA5C7" originalValue="µm">µm</normalizedToken>
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thick,
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<emphasis id="DCA2426CBF6A2881F32062FDFF4189AA" bold="true" italics="true" pageId="0" pageNumber="45">textura intricata</emphasis>
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to
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<emphasis id="FFE68170E6E85A4FBD30A9623BF5B07B" bold="true" italics="true" pageId="0" pageNumber="45">prismatica</emphasis>
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, hyaline, composed of loosely interwoven thin-walled hyphae which is frequently dichotomously branching.
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<emphasis id="233441F8D0AE006AF36B451D83EE689B" bold="true" italics="true" pageId="0" pageNumber="45">Asci</emphasis>
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(77-)79-85(-90)
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<normalizedToken id="F91BA265EDB006E90FA0F5DA6ED82A50" originalValue="×">x</normalizedToken>
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12.5-15(-17.5)
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<normalizedToken id="5A16EC1F663AD67581DD7C3F6C8D2561" originalValue="µm">µm</normalizedToken>
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, cylindrical-clavate to saccate, 8-spored, arising from croziers, containing hyaline oil globules in cytoplasm that disappear when mature, with a thick-walled conical apex; apex MLZ+ with or without 3% KOH pretreatment.
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<emphasis id="203104507AA42D299DFA714F3B49AFDB" bold="true" italics="true" pageId="0" pageNumber="45">Ascospores</emphasis>
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(32-)37-47(-57.5)
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<normalizedToken id="7AA6AE1715D8C361A8BCBF72820ACA32" originalValue="×">x</normalizedToken>
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4.5-5.5
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<normalizedToken id="0F5BC1EC69E1A36EB6AAB1BA81FCB37E" originalValue="µm">µm</normalizedToken>
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, long fusiform, with acute extremes, curved to sigmoid, occasionally constricted, thin-walled, often 5-septate, hyaline, containing large or abundant minute guttles.
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<emphasis id="F9209671C7B3D1C19D4E84FFEE41C16B" bold="true" italics="true" pageId="0" pageNumber="45">Paraphyses</emphasis>
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(70-)83-105(-115)
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<normalizedToken id="6D4D8D2CAC5543BAD662A9036A90CFA1" originalValue="×">x</normalizedToken>
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2.5-3(-4)
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<normalizedToken id="1A17BA8BB4186C0810FEA46777B3F843" originalValue="µm">µm</normalizedToken>
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, simple, occasionally branching at base, cylindrical, often becoming slightly wider toward the apex, 2-3-septate, thin-walled, hyaline, (2-)3-septate, containing long refractive vacuoles when mounted fresh in water.
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<emphasis id="57AF3430A8F2B5FA3B6AA6D1C7F8C121" bold="true" italics="true" pageId="0" pageNumber="45">Subiculum</emphasis>
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thinly developed the surface of substrates in patches, sparse to especially abundant around the mature apothecia, shiny brown; subicular hyphae straight to curved, usually swelling in a globose, 3-5
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<normalizedToken id="E97881E80BB57E84DEDE2D86E85D02E4" originalValue="µm">µm</normalizedToken>
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diam. with 0.5-1
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<normalizedToken id="F3EE3D05A73EBD16D1B7485037A4A8DE" originalValue="µm">µm</normalizedToken>
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thick-walls, septate every 15-30
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<normalizedToken id="09CE1A2AF436A26D6292EAF220A41EB7" originalValue="µm">µm</normalizedToken>
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, perpendicularly branched.
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<emphasis id="A1124D5E27B208FFE2B111EA946BFD0B" bold="true" italics="true" pageId="0" pageNumber="45">Colony</emphasis>
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of NBRC 115566 on PDA, flat to slightly convex with aerial hyphae, cottony, grayish orange (C0-20M60Y60K10) from the surface, appearing cinnamon (C20-40M80Y100K30) from reverse side, with apricot (M20-40Y60) soluble pigment uniformly diffuse in agar; aerial mycelium dense, white to pale yellow (Y10-20); crystals aggregating plate-like or small clusters, acicular, moderately abundant on colony surface and surrounding agar, 0.1-0.5 mm across, pale yellow.
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<emphasis id="785556DF5176F8366338A1075E850260" bold="true" italics="true" pageId="0" pageNumber="45">Conidiophores</emphasis>
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macronematous to mononematous, arising from subicular hyphae, straight, pale to dark brown, thick-walled, smooth, 2-3
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<normalizedToken id="66BDF907C8A28A43D29D2704E26E93E8" originalValue="µm">µm</normalizedToken>
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width;
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<emphasis id="C7FE4BB146C5F9326F9C94D3FB5502B7" bold="true" italics="true" pageId="0" pageNumber="45">phialides</emphasis>
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ampulliform, up to 15
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||
<normalizedToken id="5195E19B78A119993A128432DF961D67" originalValue="µm">µm</normalizedToken>
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||
long, 2.5-4
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<normalizedToken id="04FE0FEC243574D654970BFEE309BE93" originalValue="µm">µm</normalizedToken>
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||
width, integrated, arranged penicillately, pale brown, thick-walled, with cylindrical to wide funnel-shape collarettes of 3.5-5
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<normalizedToken id="B55F42D52111571356C92D4D0A465D58" originalValue="×">x</normalizedToken>
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||
2-3
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||
<normalizedToken id="C8F0D73D3E9F8B081CF5EDD593236FB0" originalValue="µm">µm</normalizedToken>
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;
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||
<emphasis id="ABE8C2E9EDE7B3BC3A5ACA61136D46A3" bold="true" italics="true" pageId="0" pageNumber="45">conidia</emphasis>
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aseptate, spherical to subspherical, 2-2.5
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<normalizedToken id="5EF53C7984961B4C4F1AD6C591B519CB" originalValue="µm">µm</normalizedToken>
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diam., hyaline, thin-walled.
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</paragraph>
|
||
<caption id="18A0E8F356DFC0A6B78F60D91DEFC3C0" doi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45" start="Figure 12" startId="F12">
|
||
<paragraph id="D46EBFA90A86B8ED5CADD6E97B5FAA8F" pageId="0" pageNumber="45">
|
||
<emphasis id="3BCEA000BB2D74586FF1BDAEEC646305" bold="true" pageId="0" pageNumber="45">Figure 12.</emphasis>
|
||
<taxonomicName id="46D7CFC59DB3044E0287351F6E228532" authorityName="Itagaki & Hosoya" authorityYear="2023" class="Leotiomycetes" family="Mollisiaceae" genus="Trichobelonium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobelonium miscanthi" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="miscanthi">
|
||
<emphasis id="22F10C274B4C57C8EB6F38C8356A5ED4" italics="true" pageId="0" pageNumber="45">Trichobelonium miscanthi</emphasis>
|
||
</taxonomicName>
|
||
(TNS-F-17835, holotype)
|
||
<emphasis id="966A293A66DE0536B58AD84A7D0F581C" bold="true" pageId="0" pageNumber="45">A</emphasis>
|
||
fresh apothecia on the decaying culm of
|
||
<taxonomicName id="0961E93AB924FF730CD618151F967E31" class="Ascomycetes" family="Hyaloscyphaceae" genus="Miscanthus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Miscanthus sinensis" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sinensis">
|
||
<emphasis id="AAEA06A79ED0A3EE6E46A0B365347854" italics="true" pageId="0" pageNumber="45">Miscanthus sinensis</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="99E5FC0147C8D8DFF6F1ACBBE9E9B05E" bold="true" pageId="0" pageNumber="45">B</emphasis>
|
||
dried apothecia with stromata (arrows)
|
||
<emphasis id="B92C7827060B838F84A15BA60782283F" bold="true" pageId="0" pageNumber="45">C</emphasis>
|
||
brown connective hyphae extending from the base of fresh apothecium
|
||
<emphasis id="0E0A207BB4CB0E20014F02B5F971DA21" bold="true" pageId="0" pageNumber="45">D</emphasis>
|
||
texture of stroma (in LA)
|
||
<emphasis id="E7111A1534594FE66132A93B52EAA4C2" bold="true" pageId="0" pageNumber="45">E</emphasis>
|
||
conidiophore (in LA)
|
||
<emphasis id="4DE8BA810E914DA773E928AD46146705" bold="true" pageId="0" pageNumber="45">F</emphasis>
|
||
vertical section of the apothecium (in LA)
|
||
<emphasis id="38D727D7B156D7124132D764828BB264" bold="true" pageId="0" pageNumber="45">G</emphasis>
|
||
fresh outermost cells of ectal excipulum with refractive vacuoles (in water)
|
||
<emphasis id="6F7A4C3DFE0E7CBA82B2C19CA02AF5FB" bold="true" pageId="0" pageNumber="45">H</emphasis>
|
||
ascospores (in CB/LA)
|
||
<emphasis id="137652432818EB1626C052B350413C42" bold="true" pageId="0" pageNumber="45">I</emphasis>
|
||
fresh ascospores with droplets (in water)
|
||
<emphasis id="464362B63ECA0C39945FC7EBD5CAFEE3" bold="true" pageId="0" pageNumber="45">J</emphasis>
|
||
blue-stained apical pore of ascus (in
|
||
<normalizedToken id="138B79A6F6BBCCF3AAB7A90BE1134587" originalValue="Melzer’s">Melzer's</normalizedToken>
|
||
solution after 3% KOH pretreatment)
|
||
<emphasis id="B55A07DD29700F3C7CE5E54E135AE52B" bold="true" pageId="0" pageNumber="45">K</emphasis>
|
||
croziers at the base of ascus (in CB/LA)
|
||
<emphasis id="EE5C6D484E4B999A63F22C62D3D47B0D" bold="true" pageId="0" pageNumber="45">L</emphasis>
|
||
long refractive vacuoles in fresh paraphysis (in water)
|
||
<emphasis id="3CFCAE9B97A7F3F3AABA43D4D1130FB2" bold="true" pageId="0" pageNumber="45">M</emphasis>
|
||
branching paraphysis (in CB/LA)
|
||
<emphasis id="7CCB8AFE0C64F8EED7C4564D6224F412" bold="true" pageId="0" pageNumber="45">N</emphasis>
|
||
immature ascus containing oil globes in the cytoplasm (in CB/LA)
|
||
<emphasis id="B92F1E1FBBA1EA3F089BEE03A8B7430C" bold="true" pageId="0" pageNumber="45">O</emphasis>
|
||
ascus with mature ascospores (in CB/LA)
|
||
<emphasis id="AE17B2EA4D6614FDF71B39DA8BE335FC" bold="true" pageId="0" pageNumber="45">P</emphasis>
|
||
immature ascus (in water)
|
||
<emphasis id="3C435F6824625BC9C057B80ACC68C9C4" bold="true" pageId="0" pageNumber="45">Q</emphasis>
|
||
ascus with ascospores (in water)
|
||
<emphasis id="AC2BDC6E9F3B527FA73CF9A7050221C6" bold="true" pageId="0" pageNumber="45">R</emphasis>
|
||
one month old colony on PDA
|
||
<emphasis id="2ADFF6F8B1F39472616C918E7787A748" bold="true" pageId="0" pageNumber="45">S</emphasis>
|
||
pale yellow crystals formed on the edge of the colony
|
||
<emphasis id="44700C7AC25F635A1905487F1DD35D32" bold="true" pageId="0" pageNumber="45">T</emphasis>
|
||
acicular crystals (in water)
|
||
<emphasis id="3FA73E666CB66A89323D5F6D93C4413F" bold="true" pageId="0" pageNumber="45">U</emphasis>
|
||
hyphal mass attached to the bottom of the Petri dish
|
||
<emphasis id="27366CBD97BFB702100D005715CBDCE9" bold="true" pageId="0" pageNumber="45">V-X</emphasis>
|
||
swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (
|
||
<emphasis id="FC9C926D0A12928C8757FD29FDCE59C2" bold="true" pageId="0" pageNumber="45">S</emphasis>
|
||
); 0.5 mm (
|
||
<emphasis id="095BD1C9847CF4E26C43A376DE6685FD" bold="true" pageId="0" pageNumber="45">A, B</emphasis>
|
||
); 0.25 mm (
|
||
<emphasis id="834F5A693F87AB82A9B2BB109AAD3759" bold="true" pageId="0" pageNumber="45">C</emphasis>
|
||
); 50
|
||
<normalizedToken id="8585CF41DAC51CB17F0E2D2D77F6F506" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="C138552E8C932DE09F15BCD09F2F63B1" bold="true" pageId="0" pageNumber="45">F, U</emphasis>
|
||
); 20
|
||
<normalizedToken id="8430FB59F76F040F8CD5619EA87CB385" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="0CCA2E4C452C50E314F9EE0FAEB517DA" bold="true" pageId="0" pageNumber="45">G, T</emphasis>
|
||
); 10
|
||
<normalizedToken id="F31B49FF46244157E67EE1EBB88790C8" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="B7D2E886A567B1F5F519549E8BEB2EA3" bold="true" pageId="0" pageNumber="45">D, E, H-Q, V-X</emphasis>
|
||
).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
<subSubSection id="7A45A0062888BF1C16D36646272C4B13" pageId="0" pageNumber="45" type="materials_examined">
|
||
<paragraph id="474C06790131B6D2DD3AEE20DDEB223D" pageId="0" pageNumber="45">Additional specimens examined.</paragraph>
|
||
<paragraph id="B7996D5FD0D7D7A01838E142C7879249" pageId="0" pageNumber="45">
|
||
TNS-F-30037, Hachimantai City, Iwate Pref.,
|
||
<date id="A6900D2A9F7E1030C5CEE647AE0E7FE4" value="2009-10-12">12 October 2009</date>
|
||
on decaying culm of
|
||
<taxonomicName id="E8821F44D5A9919019C0CCF26B51A190" class="Ascomycetes" family="Hyaloscyphaceae" genus="Miscanthus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Miscanthus sinensis" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sinensis">
|
||
<emphasis id="DFA2E6AE54523CA2966CC399017F5D82" italics="true" pageId="0" pageNumber="45">Miscanthus sinensis</emphasis>
|
||
</taxonomicName>
|
||
, culture NBRC 115652; TNS-F-81751, Kiritappu Wetland, Hamanaka City, Akkeshi County, Hokkaido,
|
||
<date id="AF7B24667FB17B23BA46CA55A84EB4CD" value="2019-08-29">29 August 2019</date>
|
||
, on decaying culm of
|
||
<taxonomicName id="6E036E3DD57076A5ECD1CEC887B3C372" class="Monocotyledoneae" family="Poaceae" genus="Phragmites" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Phragmites australis" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="australis">
|
||
<emphasis id="A840B1F0FA13771DEFB4A1D2B34AD524" italics="true" pageId="0" pageNumber="45">Phragmites australis</emphasis>
|
||
</taxonomicName>
|
||
; TNS-F-86581, Higashi Ward, Sapporo City, Hokkaido,
|
||
<date id="935484095871A407DF8EF14135006B8B" value="2021-08-13">13 August 2021</date>
|
||
, on decaying culm of
|
||
<taxonomicName id="02DBA8CE2E5EAA664FCACFA2F6F02F32" lsidName="Ph. australis" pageId="0" pageNumber="45" rank="species" species="australis">
|
||
<emphasis id="649A1C1A0E51CF391BEEA38C73C85D5F" italics="true" pageId="0" pageNumber="45">Ph. australis</emphasis>
|
||
</taxonomicName>
|
||
; TNS-F-86672 (culture NBRC 115667) and 86695, Yuzawa Town, Minami-uonuma County, Niigata Pref.,
|
||
<date id="E572F60C09685FD2313A53DEF14168CC" value="2021-10-17" valueMax="2021-10-31" valueMin="2021-10-17">17 and 31 October 2021</date>
|
||
(respectively), on decaying culm of
|
||
<taxonomicName id="E4448D3F4F53B6E1F408B4C2E235207B" lsidName="M. sinensis" pageId="0" pageNumber="45" rank="species" species="sinensis">
|
||
<emphasis id="77E9FC84259ACEE2D19085E90209A16A" italics="true" pageId="0" pageNumber="45">M. sinensis</emphasis>
|
||
</taxonomicName>
|
||
; TNS-F-86700, Daigenta Lake, Yuzawa Town, Minami-uonuma County, Niigata Pref.,
|
||
<date id="29CB891DF18A86292504524BFB6D3729" value="2021-10-31">31 October 2021</date>
|
||
, on decaying culm of
|
||
<taxonomicName id="72B235EDD8EC3EDCE9B84F1DDEB90C82" lsidName="M. sinensis" pageId="0" pageNumber="45" rank="species" species="sinensis">
|
||
<emphasis id="0B814677B88F719B6376E1F7E0ED22F9" italics="true" pageId="0" pageNumber="45">M. sinensis</emphasis>
|
||
</taxonomicName>
|
||
, culture NBRC 115668; TNS-F-86715, Toukamachi City, Niigata Pref.,
|
||
<date id="5960C557C60F797F213402B6C0B70450" value="2021-10-31">31 October 2021</date>
|
||
, on decaying culm of
|
||
<taxonomicName id="80E5EFF5BFE9AD938E05B2A994445363" lsidName="M. sinensis" pageId="0" pageNumber="45" rank="species" species="sinensis">
|
||
<emphasis id="15A99C9A23FEBEF122DA4CB6F770C25A" italics="true" pageId="0" pageNumber="45">M. sinensis</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="3165E15B620A29FBA243442379E55B89" pageId="0" pageNumber="45" type="notes">
|
||
<paragraph id="93FAAEDC488B4B6F7A4CFAA3CCB1A67B" pageId="0" pageNumber="45">Notes.</paragraph>
|
||
<paragraph id="472C2ED66AD61C0213696D54A4882DD5" pageId="0" pageNumber="45">
|
||
<taxonomicName id="C74F4458FC4A35D3597569459C38F84D" authorityName="Itagaki & Hosoya" authorityYear="2023" class="Leotiomycetes" family="Mollisiaceae" genus="Trichobelonium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobelonium miscanthi" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="miscanthi">
|
||
<emphasis id="4C2997AFD72EC11ADE78C87F27718913" italics="true" pageId="0" pageNumber="45">Trichobelonium miscanthi</emphasis>
|
||
</taxonomicName>
|
||
occurs with
|
||
<taxonomicName id="07644CC8B6ECDF0C2A6987358D351755" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis cinnabarina" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="cinnabarina">
|
||
<emphasis id="F6C35D0BABA58795FCAF1A1F4BF4037C" italics="true" pageId="0" pageNumber="45">Neobelonopsis cinnabarina</emphasis>
|
||
</taxonomicName>
|
||
as they share the same host,
|
||
<taxonomicName id="33D960EA74D64A304738E7273AC5884B" class="Ascomycetes" family="Discomycetes" genus="Mollisia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mollisia sinensis" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sinensis">
|
||
<emphasis id="27FCCF5A298FF8CBABE2D95899BE0F61" italics="true" pageId="0" pageNumber="45">Mollisia sinensis</emphasis>
|
||
</taxonomicName>
|
||
, and fruiting season (autumn). Brown phialides (Fig.
|
||
<figureCitation id="0835591C7F0C37B16537715AB2F6F4B1" captionStart="Figure 12" captionStartId="F12" captionText="Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." figureDoi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45">12E</figureCitation>
|
||
) and spherical conidia, regarded as asexual stage of
|
||
<taxonomicName id="F2127331211D2CA0D845DABD10F92B74" lsidName="T. miscanthi" pageId="0" pageNumber="45" rank="species" species="miscanthi">
|
||
<emphasis id="BCEC4A6AD4C9373AF91271745F325CF7" italics="true" pageId="0" pageNumber="45">T. miscanthi</emphasis>
|
||
</taxonomicName>
|
||
, were observed to accompany subiculum, but we could not induce conidial reproduction under culture.
|
||
</paragraph>
|
||
<paragraph id="ADB637CD0782BA0DC82E1BA8576A6F4F" pageId="0" pageNumber="45">
|
||
From the reverse of the two months old colony of
|
||
<taxonomicName id="E8ADE62CEB2E7C915207CB512B974C24" lsidName="T. miscanthi" pageId="0" pageNumber="45" rank="species" species="miscanthi">
|
||
<emphasis id="6FF236253B9A929B4968829C3F744E3E" italics="true" pageId="0" pageNumber="45">T. miscanthi</emphasis>
|
||
</taxonomicName>
|
||
on CMA, clumps of dark cells strongly attached to the bottom of the Petri dish (Fig.
|
||
<figureCitation id="54FDECEBE27D3DB25BBCE17A89C4C949" captionStart="Figure 12" captionStartId="F12" captionText="Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." figureDoi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45">12U</figureCitation>
|
||
) were observed. The clumps are composed of swollen cells with melanized ring. The swollen cell is usually obovoid to pyriform, sometimes lobed or hyphoid, 10-15
|
||
<normalizedToken id="622066F09AEFBA472292A046AE8C84BA" originalValue="×">x</normalizedToken>
|
||
6-10
|
||
<normalizedToken id="1058D891D87DD6B62DC9F23324BE7216" originalValue="µm">µm</normalizedToken>
|
||
, arising vertically from hyphae, thick-walled, and containing abundant guttles (Figs
|
||
<figureCitation id="BC095DF2BC718092778E51B7E14438DE" captionStart="Figure 12" captionStartId="F12" captionText="Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." figureDoi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45">12V-X</figureCitation>
|
||
,
|
||
<figureCitation id="68FE6AB51236CB4CF0DE01AEF8223229" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">14I</figureCitation>
|
||
). The brown ring structure has an outer diameter of 8-10
|
||
<normalizedToken id="90B527B560655DA34ED1806C72E64034" originalValue="µm">µm</normalizedToken>
|
||
and inner diameter of 2-3
|
||
<normalizedToken id="8F6CFACC9214747C7A03E1378566AD58" originalValue="µm">µm</normalizedToken>
|
||
, and is formed at the cell and Petri dish interface. Very similar hyphal structures were reported by
|
||
<bibRefCitation id="BD05440515F9AA0AB0D49715E8AAD582" DOI="https://doi.org/10.2307/3758475" author="Aebi, B" journalOrPublisher="Nova Hedwigia" pageId="0" pageNumber="45" pagination="49 - 112" refId="B1" refString="Aebi, B, 1972. Untersuchungen ueber Discomyceten aus der Gruppe Tapesia - Trichobelonium. Nova Hedwigia 23: 49 - 112, DOI: https://doi.org/10.2307/3758475" title="Untersuchungen ueber Discomyceten aus der Gruppe Tapesia - Trichobelonium." url="https://doi.org/10.2307/3758475" volume="23" year="1972">Aebi (1972)</bibRefCitation>
|
||
in the culture of
|
||
<taxonomicName id="96E053D0D01BA9F32D89D5FE19DC681A" lsidName="T. kneiffii" pageId="0" pageNumber="45" rank="species" species="kneiffii">
|
||
<emphasis id="1CD542813956EAECCB7481A9CE761C85" italics="true" pageId="0" pageNumber="45">T. kneiffii</emphasis>
|
||
</taxonomicName>
|
||
, but its function is unknown. The clumps of dark cells of
|
||
<taxonomicName id="EFAFB6148E1AD23BBECBFD1653C0E6C0" authorityName="P.T.W.Wong & C.Dong" authorityYear="2015" class="Leotiomycetes" family="Mollisiaceae" genus="Phialocephala" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Phialocephala bamuru" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="bamuru">
|
||
<emphasis id="DA0C399E12480E4D2E826B124E36BFF3" italics="true" pageId="0" pageNumber="45">Phialocephala bamuru</emphasis>
|
||
</taxonomicName>
|
||
P.T.W. Wong & C. Dong, known as plant pathogen, are interpreted as appressoria with infected pegs (
|
||
<bibRefCitation id="6F32A55C0A6DF4916CCE3C81EC5466FA" DOI="https://doi.org/10.1007/s13313-015-0369-0" author="Wong, P" journalOrPublisher="Australasian Plant Pathology" pageId="0" pageNumber="45" pagination="1 - 11" refId="B61" refString="Wong, P, Dong, C, Martin, P, Sharp, PJ, 2015. Fairway patch-a serious emerging disease of couch (syn. bermudagrass) [Cynodon dactylon] and kikuyu (Pennisetum clandestinum) turf in Australia caused by Phialocephala bamuru PTW Wong & C. Dong sp. nov. Australasian Plant Pathology 44 (5): 1 - 11, DOI: https://doi.org/10.1007/s13313-015-0369-0" title="Fairway patch-a serious emerging disease of couch (syn. bermudagrass) [Cynodon dactylon] and kikuyu (Pennisetum clandestinum) turf in Australia caused by Phialocephala bamuru PTW Wong & C. Dong sp. nov." url="https://doi.org/10.1007/s13313-015-0369-0" volume="44" year="2015">Wong et al. 2015</bibRefCitation>
|
||
). Although this structure may be appressorium, direct observation of the mycelium of
|
||
<taxonomicName id="6105A6780F68F78C654B3A1B7C4712C0" lsidName="T. miscanthi" pageId="0" pageNumber="45" rank="species" species="miscanthi">
|
||
<emphasis id="D3E07123FD5062E1C14C492DF379613F" italics="true" pageId="0" pageNumber="45">T. miscanthi</emphasis>
|
||
</taxonomicName>
|
||
on the host epidermis and inoculation experiments are needed to clarify whether the clamps of
|
||
<taxonomicName id="79975426740506E3D2ECDF0C1DCA36A4" lsidName="T. miscanthi" pageId="0" pageNumber="45" rank="species" species="miscanthi">
|
||
<emphasis id="821AD06043383942C3A35F38DB800D2A" italics="true" pageId="0" pageNumber="45">T. miscanthi</emphasis>
|
||
</taxonomicName>
|
||
function as an appressorium during the infection process.
|
||
</paragraph>
|
||
<caption id="FC2B2CF3CD5A204ECE67472192C7D256" doi="10.3897/mycokeys.99.90117.figure13" httpUri="https://binary.pensoft.net/fig/892429" pageId="0" pageNumber="45" start="Figure 13" startId="F13">
|
||
<paragraph id="95949C179C870FA09B6FD97D0D0067CF" pageId="0" pageNumber="45">
|
||
<emphasis id="0609D8F06512048F3DC124ED59B43CF2" bold="true" pageId="0" pageNumber="45">Figure 13.</emphasis>
|
||
Line-drawings of the
|
||
<taxonomicName id="4268746A4ECCEB1AD4D0271BD19149D2" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis" order="Helotiales" pageId="0" pageNumber="45" rank="genus">
|
||
<emphasis id="52572B29C564BB211F962B36AC3B4161" italics="true" pageId="0" pageNumber="45">Neobelonopsis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="E64AC77291D34F26E7C7E77B23776A7F" class="Leotiomycetes" family="Mollisiaceae" genus="Trichobelonium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobelonium" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="genus">
|
||
<emphasis id="3966404ED6E79C953FF7951C470F9114" italics="true" pageId="0" pageNumber="45">Trichobelonium</emphasis>
|
||
</taxonomicName>
|
||
species
|
||
<emphasis id="883D8FABC9D8D3E9ACF6B132656F3B3C" bold="true" pageId="0" pageNumber="45">I</emphasis>
|
||
vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium
|
||
<emphasis id="B33424A5367F0F220B9C67EB22B7075B" bold="true" pageId="0" pageNumber="45">II</emphasis>
|
||
asci
|
||
<emphasis id="A26E9AEFAAB312F994DAC02338FA46C5" bold="true" pageId="0" pageNumber="45">III</emphasis>
|
||
paraphyses
|
||
<emphasis id="15D5CF057992F305B0EEB96A5E1B97CF" bold="true" pageId="0" pageNumber="45">IV</emphasis>
|
||
ascospores. Scale bars: 10
|
||
<normalizedToken id="4763D66B54F6DD7EE76583CF46278C1E" originalValue="μm">μm</normalizedToken>
|
||
.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="F2B7554228D9F67F1CC83A1D54F7EA47" pageId="0" pageNumber="45">
|
||
<taxonomicName id="AFCA98FF3AE443EA0E87C858E2910C77" authorityName="Itagaki & Hosoya" authorityYear="2023" class="Leotiomycetes" family="Mollisiaceae" genus="Trichobelonium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobelonium miscanthi" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="miscanthi">
|
||
<emphasis id="0837DEB5E5C52572102F66ECE826A753" italics="true" pageId="0" pageNumber="45">Trichobelonium miscanthi</emphasis>
|
||
</taxonomicName>
|
||
resembles
|
||
<taxonomicName id="F2E2FE0BBAECF018F3D84730F83CD0BB" lsidName="T. albobarbatum" pageId="0" pageNumber="45" rank="species" species="albobarbatum">
|
||
<emphasis id="F71575A7BE14B3EBB598FD4A3C4C6DDC" italics="true" pageId="0" pageNumber="45">T. albobarbatum</emphasis>
|
||
</taxonomicName>
|
||
in remarkable oil globules in immature asci, anchoring hyphae, and saccate form of asci. Although
|
||
<taxonomicName id="CCCCB02ED553402B1B5A38F1BCAD72EE" lsidName="T. miscanthi" pageId="0" pageNumber="45" rank="species" species="miscanthi">
|
||
<emphasis id="A1277C75925D8E91102241DDAFD6CF3E" italics="true" pageId="0" pageNumber="45">T. miscanthi</emphasis>
|
||
</taxonomicName>
|
||
lacks crystals in the excipulum, the culture produced abundant acicular crystals on PDA (Fig.
|
||
<figureCitation id="944CEC526A4A42877569CE2B4A6C4E47" captionStart="Figure 12" captionStartId="F12" captionText="Figure 12. Trichobelonium miscanthi (TNS-F- 17835, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia with stromata (arrows) C brown connective hyphae extending from the base of fresh apothecium D texture of stroma (in LA) E conidiophore (in LA) F vertical section of the apothecium (in LA) G fresh outermost cells of ectal excipulum with refractive vacuoles (in water) H ascospores (in CB / LA) I fresh ascospores with droplets (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L long refractive vacuoles in fresh paraphysis (in water) M branching paraphysis (in CB / LA) N immature ascus containing oil globes in the cytoplasm (in CB / LA) O ascus with mature ascospores (in CB / LA) P immature ascus (in water) Q ascus with ascospores (in water) R one month old colony on PDA S pale yellow crystals formed on the edge of the colony T acicular crystals (in water) U hyphal mass attached to the bottom of the Petri dish V-X swollen cells with melanized appressorium-like structure. Scale bars: 1 mm (S); 0.5 mm (A, B); 0.25 mm (C); 50 μm (F, U); 20 μm (G, T); 10 μm (D, E, H-Q, V-X)." figureDoi="10.3897/mycokeys.99.90117.figure12" httpUri="https://binary.pensoft.net/fig/892428" pageId="0" pageNumber="45">12R, S, T</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</subSection>
|
||
</document> |