817 lines
65 KiB
XML
817 lines
65 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/zookeys.882.35229" ID-GBIF-Dataset="deb9492c-4982-467f-aed7-814a8a1cae23" ID-PMC="PMC6821855" ID-Pensoft-Pub="1313-2970-882-1" ID-Pensoft-UUID="328E9F4C9D425CDCA928E48D43FC02CA" ID-PubMed="31686947" ID-ZooBank="FD9F8BCE1DCD48F69B6BB48F90FEA0CF" ModsDocID="1313-2970-882-1" checkinTime="1571900115142" checkinUser="pensoft" docAuthor="Trivalairat, Poramad, Chiangkul, Krittiya & Purivirojkul, Watchariya" docDate="2019" docId="0F491ADD3720524EB87D884F7A6D6600" docLanguage="en" docName="ZooKeys 882: 1-24" docOrigin="ZooKeys 882" docSource="http://dx.doi.org/10.3897/zookeys.882.35229" docTitle="Placobdelloides sirikanchanae Trivalairat, Chiangkul & Purivirojkul, 2019, sp. nov." docType="treatment" docUuid="CE96B3D0-7E8F-47D5-8212-FCFFBFA907FF" docUuidSource="ZooBank" docVersion="6" id="328E9F4C9D425CDCA928E48D43FC02CA" lastPageNumber="1" masterDocId="328E9F4C9D425CDCA928E48D43FC02CA" masterDocTitle="Placobdelloides sirikanchanae sp. nov., a new species of glossiphoniid leech and a parasite of turtles from lower southern Thailand (Hirudinea, Rhynchobdellida)" masterLastPageNumber="24" masterPageNumber="1" pageNumber="1" updateTime="1668167944753" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Placobdelloides sirikanchanae sp. nov., a new species of glossiphoniid leech and a parasite of turtles from lower southern Thailand (Hirudinea, Rhynchobdellida)</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Trivalairat, Poramad</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Chiangkul, Krittiya</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Purivirojkul, Watchariya</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>ZooKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2019</mods:date>
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<mods:detail type="volume">
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<mods:number>882</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>1</mods:start>
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<mods:end>24</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/zookeys.882.35229</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/zookeys.882.35229</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1313-2970-882-1</mods:identifier>
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<mods:identifier type="ZooBank">FD9F8BCE1DCD48F69B6BB48F90FEA0CF</mods:identifier>
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<mods:identifier type="Pensoft-UUID">328E9F4C9D425CDCA928E48D43FC02CA</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="159834669" LSID="urn:lsid:zoobank.org:act:CE96B3D0-7E8F-47D5-8212-FCFFBFA907FF" httpUri="http://treatment.plazi.org/id/0F491ADD3720524EB87D884F7A6D6600" lastPageNumber="1" pageId="0" pageNumber="1">
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<subSubSection pageId="0" pageNumber="1" type="nomenclature">
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<paragraph pageId="0" pageNumber="1">
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<taxonomicName LSID="0F491ADD-3720-524E-B87D-884F7A6D6600" class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">Placobdelloides sirikanchanae</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Dorsal surface (upper) and ventral surface (lower) of the live holotype of Placobdelloides sirikanchanae sp. nov." figureDoi="10.3897/zookeys.882.35229.figure2" httpUri="https://binary.pensoft.net/fig/348498" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure2">Figures 2</figureCitation>
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,
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Scanning electron micrograph of anterior sucker of the paratype ZMKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing smooth surface with numerous pits." figureDoi="10.3897/zookeys.882.35229.figure3" httpUri="https://binary.pensoft.net/fig/348499" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure3">3</figureCitation>
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,
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Anterior part of the live holotype of Placobdelloides sirikanchanae sp. nov. (A) Dorsal surface showing eyes touching on somite III (white arrow) and crimson red dorsal median line (yellow arrow), (B) Ventral surface showing rolled anterior lip (yellow arrow) and eyes (white arrow)." figureDoi="10.3897/zookeys.882.35229.figure4" httpUri="https://binary.pensoft.net/fig/348500" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure4">4</figureCitation>
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,
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<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Scanning electron micrograph of dorsal surface of the paratype ZMKU-ANN- 0010 of Placobdelloides sirikanchanae sp. nov. A Dorsal surface of the complete body B Dorsal papillae (arrows) C Respiratory pores on dorsal surface (arrows)." figureDoi="10.3897/zookeys.882.35229.figure5" httpUri="https://binary.pensoft.net/fig/348501" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure5">5</figureCitation>
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,
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<figureCitation captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Scanning electron micrograph of ventral surface of the paratype ZMKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing gonopore arrangement. Upper arrow points to the male gonopore and lower arrow to the female gonopore." figureDoi="10.3897/zookeys.882.35229.figure6" httpUri="https://binary.pensoft.net/fig/348502" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure6">6</figureCitation>
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,
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<figureCitation captionStart="Figure 7" captionStartId="F7" captionText="Figure 7. Scanning electron micrograph of posterior sucker of the paratype ZMUKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing rough surface with random pit distribution (white arrows)." figureDoi="10.3897/zookeys.882.35229.figure7" httpUri="https://binary.pensoft.net/fig/348503" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure7">7</figureCitation>
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,
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<figureCitation captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Posterior part of live specimens A holotype Placobdelloides sirikanchanae sp. nov. showing dorsal crimson median line (yellow arrow) with numerous scattered dark green pigments. Bubbles are emerging from the respiratory pores (white arrows) B Placobdelloides siamensis (Oka, 1917) from Bangkok, Thailand showing yellow median line (yellow arrow) with numerous scattered yellow pigments." figureDoi="10.3897/zookeys.882.35229.figure8" httpUri="https://binary.pensoft.net/fig/348504" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure8">8</figureCitation>
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,
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<figureCitation captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Internal anatomy of Placobdelloides sirikanchanae sp. nov." figureDoi="10.3897/zookeys.882.35229.figure9" httpUri="https://binary.pensoft.net/fig/348505" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure9">9</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="materials_examined">
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<paragraph pageId="0" pageNumber="1">Material examined.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" italics="true" pageId="0" pageNumber="1">Holotype</emphasis>
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(ZMKU-ANN-0006), puddle on rubber plantation, Sadao District, Songkhla Province, Thailand (6°62'57.7"N,
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<geoCoordinate degrees="100" direction="east" minutes="41" orientation="longitude" precision="1" seconds="12.7" value="100.68686">100°41'12.7"E</geoCoordinate>
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), 21 October 2018.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="1">Paratypes</emphasis>
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(nine individuals, ZMKU-ANN-0007 to 0015), same locality data as the holotype. All collected specimens were kept in 70% alcohol and deposited at the Zoological Museum of Kasetsart University (ZMKU), Department of Zoology, Faculty of Science, Kasetsart University (
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<geoCoordinate degrees="13" direction="north" minutes="50" orientation="latitude" precision="1" seconds="53.6" value="13.848222">13°50'53.6"N</geoCoordinate>
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,
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<geoCoordinate degrees="100" direction="east" minutes="33" orientation="longitude" precision="1" seconds="47.3" value="100.56314">100°33'47.3"E</geoCoordinate>
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) on 23 November 2018.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="diagnosis">
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<paragraph pageId="0" pageNumber="1">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="1">This species can be recognized from its elongated, narrow body, crimson median dorsal line, rich dark green pigmentation, 13-17 well-developed knob papillae on each annulus, symmetrical dorsal papillae between the left and right body sides, male gonopore on XIa1/a2, female gonopore on XIa3/XIIa1, amorphous salivary glands, smooth surface with random pits inside the anterior sucker, and rugged surface with randomly distributed pits inside the posterior sucker.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="description">
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<paragraph pageId="0" pageNumber="1">Description of holotype.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">
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<emphasis italics="true" pageId="0" pageNumber="1">External morphology</emphasis>
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.
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</emphasis>
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A mature
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
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<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
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</taxonomicName>
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sp. nov. (ZMKU-ANN-0006) has an elongated, dorso-ventrally flattened, tri-annulate body (
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Dorsal surface (upper) and ventral surface (lower) of the live holotype of Placobdelloides sirikanchanae sp. nov." figureDoi="10.3897/zookeys.882.35229.figure2" httpUri="https://binary.pensoft.net/fig/348498" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure2">Figure 2</figureCitation>
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). The relaxed body length from the anterior tip to the posterior sucker is 20.83 mm. The widest point of the relaxed body (annuli 35; XV) is 4.21 mm. The cup-shaped anterior sucker diameter is 1.17 mm. The anterior sucker surface is smooth with numerous pits distributed inside (
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Scanning electron micrograph of anterior sucker of the paratype ZMKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing smooth surface with numerous pits." figureDoi="10.3897/zookeys.882.35229.figure3" httpUri="https://binary.pensoft.net/fig/348499" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure3">Figure 3</figureCitation>
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; paratype ZMKU-ANN-0009). One pair of dark spherical eyes touch each other on somite III (
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Anterior part of the live holotype of Placobdelloides sirikanchanae sp. nov. (A) Dorsal surface showing eyes touching on somite III (white arrow) and crimson red dorsal median line (yellow arrow), (B) Ventral surface showing rolled anterior lip (yellow arrow) and eyes (white arrow)." figureDoi="10.3897/zookeys.882.35229.figure4" httpUri="https://binary.pensoft.net/fig/348500" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure4">Figure 4</figureCitation>
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). The entire dorsal surface is quite rough, with 13-17 well-developed knob papillae present on each annulus (
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<figureCitation captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Scanning electron micrograph of dorsal surface of the paratype ZMKU-ANN- 0010 of Placobdelloides sirikanchanae sp. nov. A Dorsal surface of the complete body B Dorsal papillae (arrows) C Respiratory pores on dorsal surface (arrows)." figureDoi="10.3897/zookeys.882.35229.figure5" httpUri="https://binary.pensoft.net/fig/348501" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure5">Figure 5</figureCitation>
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; paratype ZMKU-ANN-0010). The dorsal papillae present a symmetrical pattern between the left and right sides of the crimson median line. The dorsal color is dark brown to greenish. The numerous respiratory pores are randomly distributed on the dorsal surface. The ventral surface is transparent and smooth. Two gonopores are located around the neck region and separated by two annuli. The male gonopore is situated in a furrow of XIa1/a2, between annuli 23 and 24 (
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<figureCitation captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Scanning electron micrograph of ventral surface of the paratype ZMKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing gonopore arrangement. Upper arrow points to the male gonopore and lower arrow to the female gonopore." figureDoi="10.3897/zookeys.882.35229.figure6" httpUri="https://binary.pensoft.net/fig/348502" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure6">Figure 6</figureCitation>
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; paratype ZMKU-ANN-0009). The female pore lies in a furrow of XIa3/XIIa1, between annuli 25 and 26. The anus opening is on the dorsal furrow anterior to the last annulus (69; XXXIV). The posterior sucker diameter is 2.08 mm. The posterior sucker surface is rough with randomly distributed pits inside (
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<figureCitation captionStart="Figure 7" captionStartId="F7" captionText="Figure 7. Scanning electron micrograph of posterior sucker of the paratype ZMUKU-ANN- 0009 of Placobdelloides sirikanchanae sp. nov. showing rough surface with random pit distribution (white arrows)." figureDoi="10.3897/zookeys.882.35229.figure7" httpUri="https://binary.pensoft.net/fig/348503" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure7">Figure 7</figureCitation>
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; paratype ZMKU-ANN-0009).
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</paragraph>
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<caption doi="10.3897/zookeys.882.35229.figure2" httpUri="https://binary.pensoft.net/fig/348498" pageId="0" pageNumber="1" start="Figure 2" startId="F2">
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">Figure 2.</emphasis>
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Dorsal surface (upper) and ventral surface (lower) of the live holotype of
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
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<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
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</taxonomicName>
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sp. nov.
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</paragraph>
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</caption>
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<caption doi="10.3897/zookeys.882.35229.figure3" httpUri="https://binary.pensoft.net/fig/348499" pageId="0" pageNumber="1" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">Figure 3.</emphasis>
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Scanning electron micrograph of anterior sucker of the paratype ZMKU-ANN-0009 of
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
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<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
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</taxonomicName>
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sp. nov. showing smooth surface with numerous pits.
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</paragraph>
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</caption>
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<caption doi="10.3897/zookeys.882.35229.figure4" httpUri="https://binary.pensoft.net/fig/348500" pageId="0" pageNumber="1" start="Figure 4" startId="F4">
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">Figure 4.</emphasis>
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Anterior part of the live holotype of
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
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<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
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</taxonomicName>
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sp. nov. (
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<emphasis bold="true" pageId="0" pageNumber="1">A</emphasis>
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) Dorsal surface showing eyes touching on somite III (white arrow) and crimson red dorsal median line (yellow arrow), (
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<emphasis bold="true" pageId="0" pageNumber="1">B</emphasis>
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) Ventral surface showing rolled anterior lip (yellow arrow) and eyes (white arrow).
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</paragraph>
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</caption>
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">
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<taxonomicName genus="Annulation" lsidName="Annulation" pageId="0" pageNumber="1" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="1">Annulation</emphasis>
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</taxonomicName>
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.
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</emphasis>
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Somites I-III are uni-annulate, IV and V are biannulate (annuli 4-7), VI-XIV are tri-annulate (annuli 8-34), XV-XVIII are uni-annulate (annuli 35-38), XIX-XXV are tri-annulate (annuli 39-59), XXVI is biannulate (annuli 60-61), and XXVII-XXXIV are uni-annulate (annuli 62-69).
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="1" type="internal morphology">
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<paragraph pageId="0" pageNumber="1">Internal morphology.</paragraph>
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" italics="true" pageId="0" pageNumber="1">Digestive system</emphasis>
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: A cylindrical slender proboscis resides in a membranous sheath that protrudes through the lip of the posterior subterminal mouth (
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<figureCitation captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Internal anatomy of Placobdelloides sirikanchanae sp. nov." figureDoi="10.3897/zookeys.882.35229.figure9" httpUri="https://binary.pensoft.net/fig/348505" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure9">Figure 9</figureCitation>
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). The proboscis sheath line is on VIa1-Xa2 (annuli 8-21). Amorphous salivary glands are packed on Xa2-XIa3 (annuli 21-25), followed by the esophageal glands on XIa1-XIIa1 (annuli 23-26). Each esophageal gland has a salivary duct that joins it to each side of the esophagus. Seven pairs of crop cecae are on XIIIa2-XXIIIa1 (annuli 30-51) with the last pair on XXIIIa1-XXXI (annuli 51-66) being diverted and extended posteriorly into four post cecae. Four pairs of diverticulated intestine are on XXIIIa1-XXXIII (annuli 51-68). A simple narrow rectum resides on XXVIa2-XXXIV (annuli 61-69) and opens dorsally at the anus in a furrow anterior of the last somite (XXXIV, annulus 69).
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</paragraph>
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<caption doi="10.3897/zookeys.882.35229.figure5" httpUri="https://binary.pensoft.net/fig/348501" pageId="0" pageNumber="1" start="Figure 5" startId="F5">
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">Figure 5.</emphasis>
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Scanning electron micrograph of dorsal surface of the paratype ZMKU-ANN-0010 of
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
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<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
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</taxonomicName>
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sp. nov.
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<emphasis bold="true" pageId="0" pageNumber="1">A</emphasis>
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Dorsal surface of the complete body
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<emphasis bold="true" pageId="0" pageNumber="1">B</emphasis>
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Dorsal papillae (arrows)
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<emphasis bold="true" pageId="0" pageNumber="1">C</emphasis>
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Respiratory pores on dorsal surface (arrows).
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</paragraph>
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</caption>
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<caption doi="10.3897/zookeys.882.35229.figure6" httpUri="https://binary.pensoft.net/fig/348502" pageId="0" pageNumber="1" start="Figure 6" startId="F6">
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<paragraph pageId="0" pageNumber="1">
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<emphasis bold="true" pageId="0" pageNumber="1">Figure 6.</emphasis>
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Scanning electron micrograph of ventral surface of the paratype ZMKU-ANN-0009 of
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<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. showing gonopore arrangement. Upper arrow points to the male gonopore and lower arrow to the female gonopore.
|
|
</paragraph>
|
|
</caption>
|
|
<caption doi="10.3897/zookeys.882.35229.figure7" httpUri="https://binary.pensoft.net/fig/348503" pageId="0" pageNumber="1" start="Figure 7" startId="F7">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Figure 7.</emphasis>
|
|
Scanning electron micrograph of posterior sucker of the paratype ZMUKU-ANN-0009 of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. showing rough surface with random pit distribution (white arrows).
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Reproductive system</emphasis>
|
|
.
|
|
</emphasis>
|
|
The male gonopore rim is thick and curled. The ejaculatory bulb on XIa2-XIIa2 (annuli 24-27) is an apple-like sac opening into the vas deferens. Two vas deferens extend posteriorly and recurve in front of post ceca anteriorly to connect to the testisacs. Six pairs of ovoid testisacs are present, and each is located in the space between a pair of crop cecae. The female gonopore rim is thinner and smoother than that of the male. The spermatheca is a rectangular sac on XIIa2-XIIIa3 (annuli 27-31), which opens into bifurcated ovisacs.
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="variation">
|
|
<paragraph pageId="0" pageNumber="1">Variation.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">External morphology</emphasis>
|
|
.
|
|
</emphasis>
|
|
The average relaxed body length is 10.77 mm long (range 7.62-40.39 mm,
|
|
<emphasis italics="true" pageId="0" pageNumber="1">N</emphasis>
|
|
= 20), and the average relaxed body width at the widest point (annuli 35, XV) is 3.96 mm (range 3.52-4.89 mm,
|
|
<emphasis italics="true" pageId="0" pageNumber="1">N</emphasis>
|
|
= 20). The average anterior sucker diameter is 1.08 mm (range 0.93-1.42 mm,
|
|
<emphasis italics="true" pageId="0" pageNumber="1">N</emphasis>
|
|
= 20). The average posterior sucker diameter is 1.94 mm (range 1.70-2.60 mm,
|
|
<emphasis italics="true" pageId="0" pageNumber="1">N</emphasis>
|
|
= 20), half the size of the maximum body width.
|
|
</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" italics="true" pageId="0" pageNumber="1">Color</emphasis>
|
|
in life is uniformly dark brown to greenish, with randomly distributed dark brown, red, yellow, and especially rich dark green pigments. There is a crimson median line present dorsally from the neck region to the posterior sucker (
|
|
<figureCitation captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Posterior part of live specimens A holotype Placobdelloides sirikanchanae sp. nov. showing dorsal crimson median line (yellow arrow) with numerous scattered dark green pigments. Bubbles are emerging from the respiratory pores (white arrows) B Placobdelloides siamensis (Oka, 1917) from Bangkok, Thailand showing yellow median line (yellow arrow) with numerous scattered yellow pigments." figureDoi="10.3897/zookeys.882.35229.figure8" httpUri="https://binary.pensoft.net/fig/348504" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure8">Figure 8</figureCitation>
|
|
). On the margin of the body, brown, dark green and yellow spots are present along the posterior sucker. The ventral surface is transparent.
|
|
</paragraph>
|
|
<caption doi="10.3897/zookeys.882.35229.figure8" httpUri="https://binary.pensoft.net/fig/348504" pageId="0" pageNumber="1" start="Figure 8" startId="F8">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Figure 8.</emphasis>
|
|
Posterior part of live specimens
|
|
<emphasis bold="true" pageId="0" pageNumber="1">A</emphasis>
|
|
holotype
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. showing dorsal crimson median line (yellow arrow) with numerous scattered dark green pigments. Bubbles are emerging from the respiratory pores (white arrows)
|
|
<emphasis bold="true" pageId="0" pageNumber="1">B</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides siamensis" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides siamensis</emphasis>
|
|
</taxonomicName>
|
|
(Oka, 1917) from Bangkok, Thailand showing yellow median line (yellow arrow) with numerous scattered yellow pigments.
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Reproductive system</emphasis>
|
|
.
|
|
</emphasis>
|
|
The length of the ovisacs depends on the reproductive stage. During the normal, non-reproductive period, ovisacs are present on XIIIa1-XIVa1 (annuli 29-32), but they can extend from XIIIa1 to XXa1 (annuli 29 to 42 (4th pair of crop cecae)) during the gestational period.
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="description">
|
|
<paragraph pageId="0" pageNumber="1">Molecular description.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Molecular comparisons based on
|
|
<emphasis italics="true" pageId="0" pageNumber="1">p</emphasis>
|
|
-distances among five specimens of
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. from a rubber plantation in the Sadao District, Songkhla Province, Thailand revealed a difference of 2.5-6.2% for 518 nucleotides of COI (GenBank MK282428-MK282432) and 1.3-3.3% for 555 nucleotides of ND1 (GenBank MK282433-MK282437) (see Tables
|
|
<tableCitation captionStartId="T2" httpUri="http://table.plazi.org/id/24B4D2E7937712073BC1682F52ACA500" pageId="0" pageNumber="1" tableUuid="24B4D2E7937712073BC1682F52ACA500">2</tableCitation>
|
|
,
|
|
<tableCitation captionStartId="T3" httpUri="http://table.plazi.org/id/4EC72978769A447907776E236ABA44B0" pageId="0" pageNumber="1" tableUuid="4EC72978769A447907776E236ABA44B0">3</tableCitation>
|
|
). The five specimens of
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
revealed differences based on
|
|
<emphasis italics="true" pageId="0" pageNumber="1">p</emphasis>
|
|
-distances of 10.4-27.7% for the COI gene and 5.4-6.9% for ND1 compared to ten specimens of
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
(GenBank AY962449, MH777415-MH777420, MN221458-MN221460 for COI, and AY962462, MH777409-MH777414, XX123456-XX13456 for ND1) collected from Bangkok and Udon Thani Province, Thailand; differences of 19.3-21.7% for the COI gene and 15.1-15.8% for ND1 compared to a specimens of
|
|
<taxonomicName lsidName="P. multistriatus" pageId="0" pageNumber="1" rank="species" species="multistriatus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. multistriatus</emphasis>
|
|
</taxonomicName>
|
|
(GenBank DQ414338 for the COI gene, and DQ414383 for the ND1 gene) collected from Louisiana, USA; and differences of 21.0-23.5% for the COI gene and 15.1-16.0% for ND1 compared to a specimen of
|
|
<taxonomicName lsidName="P. jaegerskioeldi" pageId="0" pageNumber="1" rank="species" species="jaegerskioeldi">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. jaegerskioeldi</emphasis>
|
|
</taxonomicName>
|
|
(GenBank AY692463 for COI, and AY962450 for ND1) collected from Sudan, South Africa. The Bayesian inference and maximum-likelihood trees of the COI and ND1 genes of the glossiphoniid leeches indicated high posterior probabilities and bootstrap support values for divergence between
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
and
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
(
|
|
<figureCitation captionStart="Figure 10" captionStartId="F10" captionText="Figure 10. Phylogenetic analysis of the COI-ND 1 genes of glossiphoniid leeches. The upper diagram is from the Bayesian analysis; the lower is from the maximum likelihood analysis." figureDoi="10.3897/zookeys.882.35229.figure10" httpUri="https://binary.pensoft.net/fig/348506" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure10">Figure 10</figureCitation>
|
|
).
|
|
</paragraph>
|
|
<caption doi="10.3897/zookeys.882.35229.figure9" httpUri="https://binary.pensoft.net/fig/348505" pageId="0" pageNumber="1" start="Figure 9" startId="F9">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Figure 9.</emphasis>
|
|
Internal anatomy of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov.
|
|
</paragraph>
|
|
</caption>
|
|
<caption doi="10.3897/zookeys.882.35229.figure10" httpUri="https://binary.pensoft.net/fig/348506" pageId="0" pageNumber="1" start="Figure 10" startId="F10">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Figure 10.</emphasis>
|
|
Phylogenetic analysis of the COI-ND1 genes of glossiphoniid leeches. The upper diagram is from the Bayesian analysis; the lower is from the maximum likelihood analysis.
|
|
</paragraph>
|
|
</caption>
|
|
<caption ID-Table-UUID="24B4D2E7937712073BC1682F52ACA500" httpUri="http://table.plazi.org/id/24B4D2E7937712073BC1682F52ACA500" pageId="0" pageNumber="1" start="Table 2" startId="T2">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Table 2.</emphasis>
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P</emphasis>
|
|
-distance values of COI genes within (diagonal) and among 4 species of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides</emphasis>
|
|
</taxonomicName>
|
|
including
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. identified in this study.
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<table pageId="0" pageNumber="1">
|
|
<tr pageId="0" pageNumber="1">
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">Species</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">1</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">2</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">3</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">4</th>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">1</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov.
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">2.5-6.2%</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">2</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides siamensis" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides siamensis</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">10.4-27.7%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">0.0-10.1%</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">3</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides multistriatus" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="multistriatus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides multistriatus</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">19.3-21.7%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">15.6-30.6%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">4</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides jaegerskioeldi" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="jaegerskioeldi">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides jaegerskioeldi</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">21.0-23.5%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">17.3-31.6%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">12.6%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
</table>
|
|
</paragraph>
|
|
<caption ID-Table-UUID="4EC72978769A447907776E236ABA44B0" httpUri="http://table.plazi.org/id/4EC72978769A447907776E236ABA44B0" pageId="0" pageNumber="1" start="Table 3" startId="T3">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Table 3.</emphasis>
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P</emphasis>
|
|
-distance values of ND1 genes within (diagonal) and among 4 species of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides</emphasis>
|
|
</taxonomicName>
|
|
including
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. identified in this study.
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<table pageId="0" pageNumber="1">
|
|
<tr pageId="0" pageNumber="1">
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">Species</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">1</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">2</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">3</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">4</th>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">1</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov.
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">1.3-3.3%</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">2</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides siamensis" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides siamensis</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">5.4-6.9%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">0.0-1.7%</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">3</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides multistriatus" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="multistriatus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides multistriatus</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">15.1-15.8%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">15.1-15.4%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" isEmpty="true" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">4</emphasis>
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides jaegerskioeldi" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="jaegerskioeldi">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides jaegerskioeldi</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">15.1-16.0%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">13.4-13.6%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">14.3%</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
</tr>
|
|
</table>
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="type host">
|
|
<paragraph pageId="0" pageNumber="1">Type host.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Dark-bellied leaf turtles (
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Cyclemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Cyclemys enigmatica" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="enigmatica">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Cyclemys enigmatica</emphasis>
|
|
</taxonomicName>
|
|
).
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="additional host">
|
|
<paragraph pageId="0" pageNumber="1">Additional host.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Asian leaf turtles (
|
|
<taxonomicName lsidName="C. dentata" pageId="0" pageNumber="1" rank="species" species="dentata">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. dentata</emphasis>
|
|
</taxonomicName>
|
|
).
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="habitat">
|
|
<paragraph pageId="0" pageNumber="1">Habitat.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. can be found attached on the shell surface, both the carapace and plastron, of
|
|
<taxonomicName lsidName="C. dentata" pageId="0" pageNumber="1" rank="species" species="dentata">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. dentata</emphasis>
|
|
</taxonomicName>
|
|
and
|
|
<taxonomicName lsidName="C. enigmatica" pageId="0" pageNumber="1" rank="species" species="enigmatica">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. enigmatica</emphasis>
|
|
</taxonomicName>
|
|
, which inhabit the bottom of enclosed shallow muddy puddles on rubber plantations. In the rainy season, several puddles will be connected due to an increase in the water level. Numerous small vertebrates are present in these puddles, such as small fishes or tadpoles. In the dry season, the puddles will be disconnected as the shallower waters disappear from evaporation. These aquatic ecosystems usually have low oxygen due to decomposition of leaf litter and nonflowing water.
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="laboratory observations">
|
|
<paragraph pageId="0" pageNumber="1">Laboratory observations.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Ten individuals of
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. were released into a tank with water from the type locality and equipped with an oxygen pump. All ten died almost immediately. The ten remaining specimens survived in a sealed bottle under low dissolved oxygen conditions. No ventilation (undulating movement display) was observed. After three days, they initiated copulation and deposited eggs in the sealed bottles.
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="reproduction">
|
|
<paragraph pageId="0" pageNumber="1">Reproduction.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Ten individuals of
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. displayed reproductive activity in a sealed bottle (low oxygen condition). One copulated with another individual for a few hours before they separated. The beginning of gestation was observed inside the ovisacs of both individuals (seen through the ventral surface) 2-3 days after copulation and gestation continued for approximately 3-4 days more before deposition of eggs. Round creamy-colored eggs, approximately 104-115 eggs per individual, were deposited and aggregated inside the transparent membrane beneath the venter groove of the parent (
|
|
<figureCitation captionStart="Figure 11" captionStartId="F11" captionText="Figure 11. Two-day old creamy whitish coloured eggs of Placobdelloides sirikanchanae sp. nov. after deposition." figureDoi="10.3897/zookeys.882.35229.figure11" httpUri="https://binary.pensoft.net/fig/348507" pageId="0" pageNumber="1" tableDoi="10.3897/zookeys.882.35229.figure11">Figure 11</figureCitation>
|
|
). Eggs were incubated for 3-4 days before hatching. Juveniles remained beneath the ventral groove of the parent for 10-15 additional days before leaving the parent and living on their own.
|
|
</paragraph>
|
|
<caption doi="10.3897/zookeys.882.35229.figure11" httpUri="https://binary.pensoft.net/fig/348507" pageId="0" pageNumber="1" start="Figure 11" startId="F11">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Figure 11.</emphasis>
|
|
Two-day old creamy whitish coloured eggs of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. after deposition.
|
|
</paragraph>
|
|
</caption>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="etymology">
|
|
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
The species is named in honor of Associate Professor Prapaisiri
|
|
<taxonomicName genus="Sirikanchana" lsidName="Sirikanchana" pageId="0" pageNumber="1" rank="genus">Sirikanchana</taxonomicName>
|
|
, the pioneer aquatic parasitologist of Thailand. The following common names,
|
|
<taxonomicName genus="Sirikanchana" lsidName="Sirikanchana" pageId="0" pageNumber="1" rank="genus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Sirikanchana 's leech</emphasis>
|
|
</taxonomicName>
|
|
(English),
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Pling Arjan Prapaisiri</emphasis>
|
|
(Thai), and
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Sirikanchanas Plattegel</emphasis>
|
|
(German) are suggested.
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="remarks">
|
|
<paragraph pageId="0" pageNumber="1">Remarks.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. was distinguished from
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
(based on the original description by
|
|
<bibRefCitation author="Oka, A" journalOrPublisher="Memoirs of the Asiatic Society of Bengal" pageId="0" pageNumber="1" pagination="157 - 176" refId="B45" refString="Oka, A, 1917. Zoological results of a tour in the Far East. Hirudinea. Memoirs of the Asiatic Society of Bengal 6: 157 - 176" title="Zoological results of a tour in the Far East. Hirudinea." volume="6" year="1917">Oka (1917)</bibRefCitation>
|
|
and the re-description by
|
|
<bibRefCitation DOI="https://doi.org/10.1051/parasite/2018056" author="Chiangkul, K" journalOrPublisher="Records of the Zoological Survey of India" pageId="0" pageNumber="1" refId="B11" refString="Chiangkul, K, Trivalairat, P, Purivirojkul, W, 2018. . https://doi.org/10.1051/parasite/2018056" url="https://doi.org/10.1051/parasite/2018056" year="2018">Chiangkul et al. (2018)</bibRefCitation>
|
|
) based on the following combination of characteristics (Table
|
|
<tableCitation captionStartId="T4" httpUri="http://table.plazi.org/id/7D38FAEE7A3E3ABBAA93E8AB3BA9FCA1" pageId="0" pageNumber="1" tableUuid="7D38FAEE7A3E3ABBAA93E8AB3BA9FCA1">4</tableCitation>
|
|
): elongated narrow body, smooth anterior sucker surface with numerous pits inside, 13-17 well-developed knob papillae on each annulus, 69 total annuli, dark brown to greenish color when live with a crimson median line, male gonopore between XIa1/a2 (annuli 23 and 24), female gonopore between XIa3/XIIa1 (annuli 25-26), anus opening between the last annulus and the posterior sucker, rough posterior sucker surface with random pits, and 104-115 eggs per clutch. In addition,
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
was found on
|
|
<taxonomicName lsidName="C. dentata" pageId="0" pageNumber="1" rank="species" species="dentata">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. dentata</emphasis>
|
|
</taxonomicName>
|
|
and
|
|
<taxonomicName lsidName="C. enigmatica" pageId="0" pageNumber="1" rank="species" species="enigmatica">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. enigmatica</emphasis>
|
|
</taxonomicName>
|
|
, which inhabit the bottom of enclosed shallow muddy puddles on rubber plantations, differing from
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
, in that it is found on
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Cuora" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Cuora amboinensis" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="amboinensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Cuora amboinensis</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Heosemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Heosemys annandalii" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="annandalii">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Heosemys annandalii</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Malayemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Malayemys macrocephala" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="macrocephala">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Malayemys macrocephala</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName lsidName="M. subtrijuga" pageId="0" pageNumber="1" rank="species" species="subtrijuga">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">M. subtrijuga</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName lsidName="M. khoratensis" pageId="0" pageNumber="1" rank="species" species="khoratensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">M. khoratensis</emphasis>
|
|
</taxonomicName>
|
|
, and
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Siebenrockiella" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Siebenrockiella crassicollis" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="crassicollis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Siebenrockiella crassicollis</emphasis>
|
|
</taxonomicName>
|
|
inhabiting larger, more open ponds.
|
|
</paragraph>
|
|
<caption ID-Table-UUID="7D38FAEE7A3E3ABBAA93E8AB3BA9FCA1" httpUri="http://table.plazi.org/id/7D38FAEE7A3E3ABBAA93E8AB3BA9FCA1" pageId="0" pageNumber="1" start="Table 4" startId="T4">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<emphasis bold="true" pageId="0" pageNumber="1">Table 4.</emphasis>
|
|
Comparison of morphological characters, egg number per clutch, host, and distribution of
|
|
<taxonomicName class="Clitellata" family="Glossiphoniidae" genus="Placobdelloides" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Placobdelloides sirikanchanae" order="Rhynchobdellida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Placobdelloides sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov. and
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
(Oka, 1917) in Thailand.
|
|
</paragraph>
|
|
</caption>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<table pageId="0" pageNumber="1">
|
|
<tr pageId="0" pageNumber="1">
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="2">Characters</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="2">
|
|
<taxonomicName lsidName="P. sirikanchanae" pageId="0" pageNumber="1" rank="species" species="sirikanchanae">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. sirikanchanae</emphasis>
|
|
</taxonomicName>
|
|
sp. nov.
|
|
</th>
|
|
<th colspan="2" pageId="0" pageNumber="1" rowspan="1">
|
|
<taxonomicName lsidName="P. siamensis" pageId="0" pageNumber="1" rank="species" species="siamensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">P. siamensis</emphasis>
|
|
</taxonomicName>
|
|
(Oka, 1917)
|
|
</th>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<bibRefCitation author="Oka, A" journalOrPublisher="Memoirs of the Asiatic Society of Bengal" pageId="0" pageNumber="1" pagination="157 - 176" refId="B45" refString="Oka, A, 1917. Zoological results of a tour in the Far East. Hirudinea. Memoirs of the Asiatic Society of Bengal 6: 157 - 176" title="Zoological results of a tour in the Far East. Hirudinea." volume="6" year="1917">Oka 1917</bibRefCitation>
|
|
</th>
|
|
<th colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<bibRefCitation DOI="https://doi.org/10.1051/parasite/2018056" author="Chiangkul, K" journalOrPublisher="Records of the Zoological Survey of India" pageId="0" pageNumber="1" refId="B11" refString="Chiangkul, K, Trivalairat, P, Purivirojkul, W, 2018. . https://doi.org/10.1051/parasite/2018056" url="https://doi.org/10.1051/parasite/2018056" year="2018">Chiangkul et al. 2018</bibRefCitation>
|
|
</th>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Host</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Cyclemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Cyclemys dentata" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="dentata">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Cyclemys dentata</emphasis>
|
|
</taxonomicName>
|
|
and
|
|
<taxonomicName lsidName="C. enigmatica" pageId="0" pageNumber="1" rank="species" species="enigmatica">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">C. enigmatica</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Siebenrockiella" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Siebenrockiella crassicollis" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="crassicollis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Siebenrockiella crassicollis</emphasis>
|
|
</taxonomicName>
|
|
</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Cuora" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Cuora amboinensis" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="amboinensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Cuora amboinensis</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Heosemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Heosemys annandalii" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="annandalii">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Heosemys annandalii</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName class="Reptilia" family="Geoemydidae" genus="Malayemys" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Malayemys macrocephala" order="Testudines" pageId="0" pageNumber="1" phylum="Chordata" rank="species" species="macrocephala">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Malayemys macrocephala</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName lsidName="M. subtrijuga" pageId="0" pageNumber="1" rank="species" species="subtrijuga">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">M. subtrijuga</emphasis>
|
|
</taxonomicName>
|
|
,
|
|
<taxonomicName lsidName="M. khoratensis" pageId="0" pageNumber="1" rank="species" species="khoratensis">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">M. khoratensis</emphasis>
|
|
</taxonomicName>
|
|
, and
|
|
<taxonomicName lsidName="S. crassicollis" pageId="0" pageNumber="1" rank="species" species="crassicollis">
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<emphasis italics="true" pageId="0" pageNumber="1">S. crassicollis</emphasis>
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</taxonomicName>
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</td>
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</tr>
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<tr pageId="0" pageNumber="1">
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<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Distribution</td>
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<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Sadao, Songkhla</td>
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<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Lampam, Pattalung</td>
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<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Bangkok and Udon Thani</td>
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</tr>
|
|
<tr pageId="0" pageNumber="1">
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|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Maximum relaxed length (mm)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">40.39</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">15.00</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">25.00</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Maximum relaxed widest width (mm)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">4.89</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">4.00</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">5.57</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Body shape</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Elongated narrow</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Elongated oval</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Elongated oval</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Eye location</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">III</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">III</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">III</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Anterior sucker diameter (mm)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">1.08</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">2.50</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">1.86</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Anterior sucker surface</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Smooth with numerous pits</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Smooth with numerous pit</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Position of proboscis opening</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Posterior subterminal</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Posterior subterminal</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Posterior subterminal</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Number of dorsal papillae on each annulus</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">13-17</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">22-27</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">5-9</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Shape of dorsal papillae</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Well-developed knob shape</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Cone shape</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Well-developed longitudinal rod shape</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Total annuli</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">69</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">67</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">69</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Live dorsal color</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Dark brown greenish with crimson median line</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Uniform gray with faint brown median line (in alcohol)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Brownish gray with yellow median line and four pairs of scarlet dots</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Male gonopore location</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">XIa1/a2 (annuli 23/24)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">XIa3/XIIa1 (annuli 25/26)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Xa3/XIa1 (annuli 22/23)</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Ejaculatory bulb</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Apple-like sac</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Glasses-like sac</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Female gonopore location</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">XIa3/XIIa1 (annuli 25/26)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">XIIa2/a3 (annuli 27/28)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">XIa2/a3 (annuli 24/25)</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Spermatheca</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Rectangular sac</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Slender sac</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Anus location</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Between last annuli and posterior sucker</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Between last annuli and posterior sucker</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Between last annuli and posterior sucker</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Posterior sucker diameter (mm)</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">1.94</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">3.00</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">3.00</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Posterior sucker surface</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Rough with random scattered pits</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Smooth with random scattered pits</td>
|
|
</tr>
|
|
<tr pageId="0" pageNumber="1">
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">Eggs per clutch</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">104-115</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">-</td>
|
|
<td colspan="1" pageId="0" pageNumber="1" rowspan="1">173-412</td>
|
|
</tr>
|
|
</table>
|
|
</paragraph>
|
|
</subSubSection>
|
|
</treatment>
|
|
</document> |