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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.39.26914" ID-GBIF-Dataset="eda7a41a-f75a-4daa-ad76-1963372cba89" ID-PMC="PMC6160862" ID-Pensoft-Pub="1314-4049-39-97" ID-PubMed="30271260" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-39-97" ModsDocOrigin="MycoKeys 39" ModsDocTitle="High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described" checkinTime="1555333493008" checkinUser="pensoft" docAuthor="Yang, Qin, Fan, Xin-Lei, Guarnaccia, Vladimiro &amp; Tian, Cheng-Ming" docDate="2018" docId="1F9A5CA8D53E8390D607DB041C073BBF" docLanguage="en" docName="MycoKeys 39: 97-149" docOrigin="MycoKeys 39" docSource="http://dx.doi.org/10.3897/mycokeys.39.26914" docTitle="Diaporthe fraxinicola C. M. Tian &amp; Q. Yang, sp. nov." docType="treatment" docVersion="4" lastPageNumber="97" masterDocId="FFD50050FFFDA55CFF8A4E3BFFF3FFBB" masterDocTitle="High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described" masterLastPageNumber="149" masterPageNumber="97" pageNumber="97" updateTime="1668136060170" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Fan, Xin-Lei</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Guarnaccia, Vladimiro</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tian, Cheng-Ming</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>39</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>97</mods:start>
<mods:end>149</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.39.26914</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.39.26914</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-39-97</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="156202289" LSID="urn:lsid:plazi:treatment:1F9A5CA8D53E8390D607DB041C073BBF" httpUri="http://treatment.plazi.org/id/1F9A5CA8D53E8390D607DB041C073BBF" lastPageNumber="97" pageId="0" pageNumber="97">
<subSubSection pageId="0" pageNumber="97" type="nomenclature">
<paragraph pageId="0" pageNumber="97">
<taxonomicName LSID="MB824711" authority="C. M. Tian &amp; Q. Yang" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe fraxinicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="fraxinicola">Diaporthe fraxinicola C.M. Tian &amp; Q. Yang</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="97">sp. nov.</taxonomicNameLabel>
Figure 13
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="diagnosis">
<paragraph pageId="0" pageNumber="97">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="97">
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe fraxinicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="fraxinicola">Diaporthe fraxinicola</taxonomicName>
can be distinguished from the closely related species
<taxonomicName lsidName="D. oraccinii" pageId="0" pageNumber="97" rank="species" species="oraccinii">D. oraccinii</taxonomicName>
and
<taxonomicName lsidName="D. acerigena" pageId="0" pageNumber="97" rank="species" species="acerigena">D. acerigena</taxonomicName>
(described above) based on ITS, tef1 and tub2 loci.
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe fraxinicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="fraxinicola">Diaporthe fraxinicola</taxonomicName>
differs from
<taxonomicName lsidName="D. oraccinii" pageId="0" pageNumber="97" rank="species" species="oraccinii">D. oraccinii</taxonomicName>
in larger alpha conidia and from
<taxonomicName lsidName="D. acerigena" pageId="0" pageNumber="97" rank="species" species="acerigena">D. acerigena</taxonomicName>
in wider alpha conidia.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="holotype">
<paragraph pageId="0" pageNumber="97">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="97">
CHINA. Shaanxi Province: Zhashui city, Niubeiliang Reserve, on symptomatic twigs of
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Fraxinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Fraxinus chinensis" order="Lamiales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="chinensis">Fraxinus chinensis</taxonomicName>
, 7 July 2017, Q. Yang (holotype: BJFC-S1495; ex-type culture: CFCC 52582).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="etymology">
<paragraph pageId="0" pageNumber="97">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="97">
Named after the host genus on which it was collected,
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Fraxinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Fraxinus" order="Lamiales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="genus">Fraxinus</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="description">
<paragraph pageId="0" pageNumber="97">Description.</paragraph>
<paragraph pageId="0" pageNumber="97">
Conidiomata pycnidial, immersed in bark, scattered, slightly erumpent through the bark surface, nearly flat, discoid, with a single locule. Ectostromatic disc grey to dark brown, circular to ovoid, one ostiole per disc, 150-325
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. Locule circular, undivided, 275-480
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. Conidiophores 10.5-17.5
<normalizedToken originalValue="×">x</normalizedToken>
2.1-3.2
<normalizedToken originalValue="μm">μm</normalizedToken>
, hyaline, branched, cylindrical to clavate, straight, tapering towards the apex. Alpha conidia hyaline, aseptate, ellipsoidal to oval, 2-3-guttulate, rounded at both ends, 7-10
<normalizedToken originalValue="×">x</normalizedToken>
2.9-3.2
<normalizedToken originalValue="μm">μm</normalizedToken>
(av. = 8.5
<normalizedToken originalValue="×">x</normalizedToken>
3
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 30). Beta conidia hyaline, filiform, straight or hamate, eguttulate, aseptate, base subtruncate, tapering towards one apex, 19-29.5
<normalizedToken originalValue="×">x</normalizedToken>
1.4
<normalizedToken originalValue="µm">µm</normalizedToken>
(av. = 24.5
<normalizedToken originalValue="×">x</normalizedToken>
1.4
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30).
</paragraph>
<caption pageId="0" pageNumber="97">
<paragraph pageId="0" pageNumber="97">
Figure 13.
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe fraxinicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="fraxinicola">Diaporthe fraxinicola</taxonomicName>
(CFCC 52582)
<normalizedToken originalValue="AB">A-B</normalizedToken>
Habit of conidiomata on branches C Transverse section of conidioma D Longitudinal section of conidioma E Alpha conidia F Beta conidia G Culture on PDA and conidiomata. Scale bars: 500
<normalizedToken originalValue="μm">μm</normalizedToken>
(B), 200
<normalizedToken originalValue="μm">μm</normalizedToken>
(C), 100
<normalizedToken originalValue="μm">μm</normalizedToken>
(D), 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="EF">E-F</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="description">
<paragraph pageId="0" pageNumber="97">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="97">Cultures incubated on PDA at 25 °C in darkness. Colony originally flat with white aerial mycelium, becoming yellowish, dense and felted aerial mycelium with age, with visible solitary or aggregated conidiomata at maturity.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="materials_examined">
<paragraph pageId="0" pageNumber="97">Additional material examined.</paragraph>
<paragraph pageId="0" pageNumber="97">
CHINA. Shaanxi Province: Zhashui city, Niubeiliang Reserve, on symptomatic twigs of
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Fraxinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Fraxinus chinensis" order="Lamiales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="chinensis">Fraxinus chinensis</taxonomicName>
, 7 July 2017, Q. Yang, living culture CFCC 52583 (BJFC-S1496).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="notes">
<paragraph pageId="0" pageNumber="97">Notes.</paragraph>
<paragraph pageId="0" pageNumber="97">
This new species is introduced as molecular data, shows it to be a distinct clade with high support (ML/BI=100/1) and it appears most closely related to
<taxonomicName lsidName="D. oraccinii" pageId="0" pageNumber="97" rank="species" species="oraccinii">D. oraccinii</taxonomicName>
and
<taxonomicName lsidName="D. acerigena" pageId="0" pageNumber="97" rank="species" species="acerigena">D. acerigena</taxonomicName>
.
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe fraxinicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="fraxinicola">Diaporthe fraxinicola</taxonomicName>
can be distinguished from
<taxonomicName lsidName="D. oraccinii" pageId="0" pageNumber="97" rank="species" species="oraccinii">D. oraccinii</taxonomicName>
by 22 nucleotides in concatenated alignment, in which 6 were distinct in the ITS region, 8 in the tef1 region and 8 in the tub2 region; from
<taxonomicName lsidName="D. acerigena" pageId="0" pageNumber="97" rank="species" species="acerigena">D. acerigena</taxonomicName>
by 27 nucleotides in concatenated alignment, in which 11 were distinct in the ITS region, 3 in the tef1 region and 13 in the tub2 region. Morphologically,
<taxonomicName lsidName="D. fraxinicola" pageId="0" pageNumber="97" rank="species" species="fraxinicola">D. fraxinicola</taxonomicName>
differs from
<taxonomicName lsidName="D. oraccinii" pageId="0" pageNumber="97" rank="species" species="oraccinii">D. oraccinii</taxonomicName>
in longer and larger alpha conidia (7-10
<normalizedToken originalValue="×">x</normalizedToken>
2.9-3.2 vs. 5.5-7.5
<normalizedToken originalValue="×">x</normalizedToken>
0.5-2
<normalizedToken originalValue="μm">μm</normalizedToken>
); differs from
<taxonomicName lsidName="D. acerigena" pageId="0" pageNumber="97" rank="species" species="acerigena">D. acerigena</taxonomicName>
in larger alpha conidia (2.9-3.2 vs. 2.1-2.9
<normalizedToken originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation author="Gao, YH" journalOrPublisher="Systematics and Biodiversity" pageId="0" pageNumber="97" pagination="102 - 117" title="Unravelling Diaporthe species associated with Camellia." url="https://doi.org/10.1080/14772000.2015.1101027" volume="14" year="2016">Gao et al. 2016</bibRefCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>