127 lines
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127 lines
12 KiB
XML
<document id="066C25D1FEFF99CEAB8956738671691E" ID-CLB-Dataset="298201" ID-DOI="10.1590/1519-6984.232434" ID-GBIF-Dataset="28d5ed5b-8f80-42a8-8c0b-31699a1e913d" ID-ISSN="1678-4375" ID-Zenodo-Dep="11494228" IM.bibliography_approvedBy="juliana" IM.illustrations_approvedBy="juliana" IM.metadata_approvedBy="juliana" IM.tables_approvedBy="juliana" IM.taxonomicNames_approvedBy="juliana" IM.treatments_approvedBy="juliana" checkinTime="1717620484603" checkinUser="felipe" docAuthor="Naseer, Q. A., Xue, X., Wang, X., Dang, S., Din, S. U. & Jamil, J." docDate="2022" docId="03CB9026FFF0B719DC9BFE95E2B0FB3B" docLanguage="en" docName="BrazJBiol.82.e232434.pdf" docOrigin="Brazilian Journal of Biology (e 232434) 82" docSource="http://dx.doi.org/10.1590/1519-6984.232434" docStyle="DocumentStyle:1DDA4544BCC4C9B5C0D0325B81F0E249.1:BrazJBiol.2022-.journal_article" docStyleId="1DDA4544BCC4C9B5C0D0325B81F0E249" docStyleName="BrazJBiol.2022-.journal_article" docStyleVersion="1" docTitle="Lactobacillus bulgaricus" docType="treatment" docVersion="3" lastPageNumber="2" masterDocId="FFF2E85EFFF1B718DE20FFF1E106FFBD" masterDocTitle="Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential" masterLastPageNumber="8" masterPageNumber="1" pageNumber="2" updateTime="1717783119523" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:titleInfo id="AFD1ADB9BE7A54F5054A54C0BE092449">
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<mods:title id="5F702561842F5EF965031CBA3ECAA331">Synthesis of silver nanoparticles using Lactobacillus bulgaricus and assessment of their antibacterial potential</mods:title>
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<mods:name id="ABDAE874EC160151D504EC57F9627F3C" type="personal">
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<mods:namePart id="372AFC69582DC9AF9D4759C01A24FE2C">Naseer, Q. A.</mods:namePart>
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<mods:affiliation id="EA2120163DB69C78F67323080FC04DCD">Jiangsu University, The Affiliated Hospital, Department of General Surgery, Zhenjiang, Jiangsu, China & Jiangsu University, School of Medicine, Department of Immunology and Institute of Laboratory Clinical Diagnostics, Zhenjiang, Jiangsu, China</mods:affiliation>
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<mods:namePart id="F4E10FB138DD9472A5AE4C6678E44318">Xue, X.</mods:namePart>
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<mods:affiliation id="065E8B3206132105516CA30514850695">Pucheng Hospital, Department of General Surgery, Pucheng, Shanxi, China</mods:affiliation>
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</mods:name>
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<mods:name id="BD56A636C78F91532D38534CCC869C00" type="personal">
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<mods:roleTerm id="9A2C679F7E6732DD8244F2D283229563">Author</mods:roleTerm>
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<mods:namePart id="A6AEEEEB22A4BDFD03534ABE63044D25">Wang, X.</mods:namePart>
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<mods:affiliation id="B21B0827D6AE59971CCA32E3892484C1">Pucheng Hospital, Department of General Surgery, Pucheng, Shanxi, China</mods:affiliation>
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</mods:name>
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<mods:name id="5B923BF2DB795330BAB4199E827D1B0D" type="personal">
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<mods:namePart id="2107460023A206FDFEC7D050F310700A">Dang, S.</mods:namePart>
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<mods:affiliation id="89627F64050B878850DF83AB249811F6">Jiangsu University, The Affiliated Hospital, Department of General Surgery, Zhenjiang, Jiangsu, China & Pucheng Hospital, Department of General Surgery, Pucheng, Shanxi, China</mods:affiliation>
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</mods:name>
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<mods:namePart id="1C996E268CBADCB136C1D3694B210E0D">Din, S. U.</mods:namePart>
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<mods:affiliation id="586388AA4533C3C592162FAF0136A2A2">Quaid I Azam University, Department of Microbiology, Islamabad, Pakistan & University of Swabi, Department of Microbiology, Swabi, KP, Pakistan</mods:affiliation>
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<mods:namePart id="F83BB233597C66A860676C01981ECE7A">Jamil, J.</mods:namePart>
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<mods:affiliation id="65FFEA058FEDA98305134CC5C5F2E25D">University of Swabi, Department of Microbiology, Swabi, KP, Pakistan</mods:affiliation>
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<mods:typeOfResource id="52DDEE88DFF727BE438354C247DE8054">text</mods:typeOfResource>
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<mods:title id="F042885106FA6C14247641A66B89F0B3">Brazilian Journal of Biology</mods:title>
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<mods:part id="5623887912D80A25EDC774F645ABC69D">
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<mods:date id="60D407A106E7A5E92EE50CFA40AB2779">2022</mods:date>
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<mods:title id="10DE21B7F711B8FE842914321C49EDEF">e 232434</mods:title>
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<mods:number id="5265E3D8D1B1942EEE0C5AAE1EFD064F">2020-07-13</mods:number>
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<mods:detail id="55E5F84F9049A9A5AE0E97EF6E2D1A73" type="volume">
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<mods:number id="86B98B5571087CFAC3191515E220283C">82</mods:number>
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<mods:extent id="CF3CEEE120F73B32CA60FF4253629CB3" unit="page">
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<mods:start id="ADAD0B826BBF063150E8554801CAF13B">1</mods:start>
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<mods:end id="3EB39D36A969C77E03BD879D44420912">8</mods:end>
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<mods:url id="71EF9DA2DBA3276AC6680A61B0DDBBEA">http://dx.doi.org/10.1590/1519-6984.232434</mods:url>
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<mods:classification id="92E5A76B36C847ED45513E2DD9F97116">journal article</mods:classification>
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<mods:identifier id="15336ED28213878E373602A9BA8A919B" type="CLB-Dataset">298201</mods:identifier>
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<mods:identifier id="1F0C95F1F6C1E92B795297BDB8AE7D29" type="DOI">10.1590/1519-6984.232434</mods:identifier>
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<mods:identifier id="9A2808A6248223F018D297FCC8C61EF7" type="GBIF-Dataset">28d5ed5b-8f80-42a8-8c0b-31699a1e913d</mods:identifier>
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<mods:identifier id="8C7168BB627FA9F464A7AE77C55AA5D6" type="ISSN">1678-4375</mods:identifier>
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<mods:identifier id="AA60A4B101D399FCC3F0F2DE6C3B2035" type="Zenodo-Dep">11494228</mods:identifier>
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<treatment id="03CB9026FFF0B719DC9BFE95E2B0FB3B" ID-DOI="http://doi.org/10.5281/zenodo.11520937" ID-GBIF-Taxon="232213365" ID-Zenodo-Dep="11520937" LSID="urn:lsid:plazi:treatment:03CB9026FFF0B719DC9BFE95E2B0FB3B" httpUri="http://treatment.plazi.org/id/03CB9026FFF0B719DC9BFE95E2B0FB3B" lastPageNumber="2" pageId="1" pageNumber="2">
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<subSubSection id="C37872BBFFF0B719DC9BFE95E5CCFEC4" box="[699,1226,356,377]" pageId="1" pageNumber="2" type="nomenclature">
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<paragraph id="8BDD2130FFF0B719DC9BFE95E5CCFEC4" blockId="1.[698,1249,356,724]" box="[699,1226,356,377]" pageId="1" pageNumber="2">
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<heading id="D095965CFFF0B719DC9BFE95E5CCFEC4" box="[699,1226,356,377]" centered="true" fontSize="8" level="2" pageId="1" pageNumber="2" reason="3">
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<emphasis id="B916FD22FFF0B719DC9BFE95E5CCFEC4" box="[699,1226,356,377]" italics="true" pageId="1" pageNumber="2">
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2.1.
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<taxonomicName id="4C625AB3FFF0B719DCC2FE95E2C2FEC4" ID-CoL="3RSQ9" authority="(Orla-Jensen, 1919) Rogosa & Hansen, 1971" box="[738,964,356,377]" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="1" pageNumber="2" phylum="Firmicutes" rank="species" species="bulgaricus">Lactobacillus bulgaricus</taxonomicName>
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: isolation and identification
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C37872BBFFF0B719DCF9FE78E2B0FB3B" pageId="1" pageNumber="2" type="description">
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<paragraph id="8BDD2130FFF0B719DCF9FE78E5D9FD69" blockId="1.[698,1249,356,724]" pageId="1" pageNumber="2">
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The
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<taxonomicName id="4C625AB3FFF0B719DD20FE79E2DFFE20" box="[768,985,392,413]" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="1" pageNumber="2" phylum="Firmicutes" rank="species" species="bulgaricus">
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<emphasis id="B916FD22FFF0B719DD20FE79E2DFFE20" box="[768,985,392,413]" italics="true" pageId="1" pageNumber="2">Lactobacillus bulgaricus</emphasis>
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</taxonomicName>
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was isolated from raw milk, sample was 10 fold diluted ranging from 10
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<superScript id="7C178C78FFF0B719DA6EFE55E55CFE0D" attach="left" box="[1102,1114,420,432]" fontSize="5" pageId="1" pageNumber="2">-1</superScript>
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to10
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<superScript id="7C178C78FFF0B719DAACFE55E598FE0D" attach="none" box="[1164,1182,420,432]" fontSize="5" pageId="1" pageNumber="2">-10</superScript>
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. At the end of dilution, 100 µL of sample was shifted aseptically with the help of micropipette to the Rogasa, and Sharpe (MRS) agar plates by using sterile glass spreader. Afterward, plates were stored at static incubator for 24 hrs at 37 °C. After incubation, the
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<typeStatus id="54D99F92FFF0B719DDA9FDC5E2B8FDFA" box="[905,958,564,583]" pageId="1" pageNumber="2">types</typeStatus>
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and number of colonies were counted and examined. The isolated colonies were subcultured in triplicate on freshly prepared MRS plates for purpose to obtain pure culture. The pure strain keep at 4 °C for further analysis.
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<taxonomicName id="4C625AB3FFF0B719DD57FD52E555FD05" box="[887,1107,675,696]" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="1" pageNumber="2" phylum="Firmicutes" rank="species" species="bulgaricus">
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<emphasis id="B916FD22FFF0B719DD57FD52E555FD05" box="[887,1107,675,696]" italics="true" pageId="1" pageNumber="2">Lactobacillus bulgaricus</emphasis>
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</taxonomicName>
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was identified by mean of biochemical and morphological characteristics.
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</paragraph>
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<paragraph id="8BDD2130FFF0B719DC9BFD0BE5A8FCB2" blockId="1.[698,1247,762,1158]" box="[699,1198,762,783]" pageId="1" pageNumber="2">
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<emphasis id="B916FD22FFF0B719DC9BFD0BE5A8FCB2" box="[699,1198,762,783]" italics="true" pageId="1" pageNumber="2">
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2.2. Synthesis of AgNPs using
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<taxonomicName id="4C625AB3FFF0B719DDEEFD0BE5A8FCB2" box="[974,1198,762,783]" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="1" pageNumber="2" phylum="Firmicutes" rank="species" species="bulgaricus">Lactobacillus bulgaricus</taxonomicName>
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</emphasis>
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</paragraph>
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<paragraph id="8BDD2130FFF0B719DCF9FCEEE2B0FB3B" blockId="1.[698,1247,762,1158]" pageId="1" pageNumber="2">
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A loop full pure culture of
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<taxonomicName id="4C625AB3FFF0B719DDF4FCEFE5B5FC8E" box="[980,1203,798,819]" class="Bacilli" family="Lactobacillaceae" genus="Lactobacillus" kingdom="Bacteria" order="Lactobacillales" pageId="1" pageNumber="2" phylum="Firmicutes" rank="species" species="bulgaricus">
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<emphasis id="B916FD22FFF0B719DDF4FCEFE5B5FC8E" box="[980,1203,798,819]" italics="true" pageId="1" pageNumber="2">Lactobacillus bulgaricus</emphasis>
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</taxonomicName>
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was inoculated in MRS broth and incubated overnight at 37 °C. After incubation the biomass was centrifuged at 5000 rpm for 10 minutes and transfers to sterile bottle and preserved at 4 °C for further use in silver nanoparticle synthesis. The synthesis of AgNPs needs precursor, for which silver nitrate (AgNO 3) was used. 10 mL of 1 mMAgNO 3 solution was prepared in 1 mL of cell free supernatant. To obtained reaction the solution was incubated overnight at room temperature. Control solutions were also prepared for comparison, i.e., 10 mL of AgNO
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<subScript id="17E62375FFF0B719DDC7FBB6E2E8FBEE" attach="left" box="[999,1006,1095,1107]" fontSize="5" pageId="1" pageNumber="2">3</subScript>
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and 10 mL of distal water separately. The synthesis of AgNPs was determined by color changes of solution.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |