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<document id="EA91CF981F8EA90D75B74EB5B2FD78D2" ID-CLB-Dataset="8973" ID-DOI="10.11646/zootaxa.4820.1.3" ID-GBIF-Dataset="2764e649-f308-4e3b-aaa0-9d29e81a06f2" ID-ISSN="1175-5326" ID-Zenodo-Dep="4397253" ID-ZooBank="E31607E8-A25D-44FA-AE5A-299BE6E2D0D6" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1609206133287" checkinUser="plazi" docAuthor="Mukherjee, Tuhar, Mukherjee, Bindarika &amp; Hazra, Niladri" docDate="2020" docId="487687C5FFCEFFBDFF05FB59FCB86FE5" docLanguage="en" docName="zootaxa.4820.1.3.pdf" docOrigin="Zootaxa 4820 (1)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Polypedilum (Polypedilum) tamanigrum Sasa 1983" docType="treatment" docVersion="9" lastPageNumber="43" masterDocId="B44FFFBDFFC2FFB1FF92FFDFFF856976" masterDocTitle="Revision of the Oriental species of Polypedilum Kieffer (Diptera: Chironomidae) with their phylogenetic relationship" masterLastPageNumber="69" masterPageNumber="31" pageNumber="43" updateTime="1698800547221" updateUser="plazi" zenodo-license-document="CLOSED">
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<mods:title id="A9D4BE6EC2C1E6344C83C8D7B8A556DF">Revision of the Oriental species of Polypedilum Kieffer (Diptera: Chironomidae) with their phylogenetic relationship</mods:title>
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<mods:namePart id="E18614387F883B78C05686586A2D4CA2">Mukherjee, Tuhar</mods:namePart>
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<mods:namePart id="75492B0F3F8B9D5698C51E551986AFC6">Mukherjee, Bindarika</mods:namePart>
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<mods:affiliation id="15E9C8666E37DE849124E7D96950D4CC">Entomology Research Unit, Department of Zoology, The University of Burdwan, Burdwan, India &amp; bindabdn @ gmail. com; https: // orcid. org / 0000 - 0002 - 9792 - 7931</mods:affiliation>
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<mods:namePart id="4A6B355FE6ACF94CF95AE8B8BD8D78F4">Hazra, Niladri</mods:namePart>
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<mods:affiliation id="DE0696C966C0267D2369E77EF5E3C871">Entomology Research Unit, Department of Zoology, The University of Burdwan, Burdwan, India &amp; hazra. niladri @ gmail. com; https: // orcid. org / 0000 - 0001 - 8149 - 9445</mods:affiliation>
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<subSubSection id="88C56558FFCEFFBDFF05FB59FD976DB3" pageId="12" pageNumber="43" type="nomenclature">
<paragraph id="C06036D3FFCEFFBDFF05FB59FC8D6DD6" blockId="12.[151,776,1158,1221]" box="[151,776,1158,1185]" pageId="12" pageNumber="43">
<heading id="9B2881BFFFCEFFBDFF05FB59FC8D6DD6" bold="true" box="[151,776,1158,1185]" fontSize="11" level="1" pageId="12" pageNumber="43" reason="1">
<taxonomicName id="07DF4D50FFCEFFBDFF05FB59FC8D6DD6" ID-CoL="8DGC4" authority="Sasa, 1983" authorityName="Sasa" authorityYear="1983" box="[151,776,1158,1185]" class="Insecta" family="Chironomidae" genus="Polypedilum" kingdom="Animalia" order="Diptera" pageId="12" pageNumber="43" phylum="Arthropoda" rank="species" species="tamanigrum" subGenus="Polypedilum">
<emphasis id="F2ABEAC1FFCEFFBDFF05FB59FC8D6DD6" bold="true" box="[151,776,1158,1185]" pageId="12" pageNumber="43">
<emphasis id="F2ABEAC1FFCEFFBDFF05FB59FEAA6DD7" bold="true" box="[151,303,1158,1185]" italics="true" pageId="12" pageNumber="43">Polypedilum</emphasis>
(
<emphasis id="F2ABEAC1FFCEFFBDFED2FB59FE5D6DD7" bold="true" box="[320,472,1158,1185]" italics="true" pageId="12" pageNumber="43">Polypedilum</emphasis>
)
<emphasis id="F2ABEAC1FFCEFFBDFE7AFB58FDFB6DD7" bold="true" box="[488,638,1159,1185]" italics="true" pageId="12" pageNumber="43">tamanigrum</emphasis>
<bibRefCitation id="A44E4B22FFCEFFBDFD14FB59FC8D6DD6" author="Sasa, M." box="[646,776,1158,1184]" pageId="12" pageNumber="43" pagination="1 - 67" refId="ref37571" refString="Sasa, M. (1983) Studies on chironomid midges of the Tama River. Part 5. An observation on the distribution of Chironominae along the main stream in June, with description of 15 new species. Research Report from the National Institute for Environmental Studies, 43, 1 - 67." type="journal article" year="1983">Sasa, 1983</bibRefCitation>
</emphasis>
</taxonomicName>
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</paragraph>
<paragraph id="C06036D3FFCEFFBDFF05FB74FD976DB3" blockId="12.[151,776,1158,1221]" box="[151,530,1195,1221]" pageId="12" pageNumber="43">
GenBank accession no.
<accessionNumber id="DF8CAB30FFCEFFBDFE03FB74FD976DB3" box="[401,530,1195,1221]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/MN637874" pageId="12" pageNumber="43">MN637874</accessionNumber>
</paragraph>
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<paragraph id="C06036D3FFCEFFBDFF05FB30FD576C71" blockId="12.[151,722,1263,1287]" box="[151,722,1263,1287]" pageId="12" pageNumber="43">
<treatmentCitationGroup id="E0CF11FDFFCEFFBDFF05FB30FD576C71" box="[151,722,1263,1287]" pageId="12" pageNumber="43">
<treatmentCitation id="417E10C2FFCEFFBDFF05FB30FD4B6C71" author="Sasa, M." box="[151,718,1263,1287]" page="14" pageId="12" pageNumber="43" year="1983">
<taxonomicName id="07DF4D50FFCEFFBDFF05FB30FD4B6C71" ID-CoL="8DGC4" authority="Sasa, 1983: 14" authorityName="Sasa" authorityPageNumber="14" authorityYear="1983" box="[151,718,1263,1287]" class="Insecta" family="Chironomidae" genus="Polypedilum" kingdom="Animalia" order="Diptera" pageId="12" pageNumber="43" phylum="Arthropoda" rank="species" species="tamanigrum" subGenus="Polypedilum">
<emphasis id="F2ABEAC1FFCEFFBDFF05FB30FE926C71" box="[151,279,1263,1287]" italics="true" pageId="12" pageNumber="43">Polypedilum</emphasis>
(
<emphasis id="F2ABEAC1FFCEFFBDFEB4FB30FE236C71" box="[294,422,1263,1287]" italics="true" pageId="12" pageNumber="43">Polypedilum</emphasis>
)
<emphasis id="F2ABEAC1FFCEFFBDFE27FB2FFDB46C71" box="[437,561,1264,1287]" italics="true" pageId="12" pageNumber="43">tamanigrum</emphasis>
<bibRefCitation id="A44E4B22FFCEFFBDFDA5FB30FD4B6C71" author="Sasa, M." box="[567,718,1263,1287]" pageId="12" pageNumber="43" pagination="1 - 67" refId="ref37571" refString="Sasa, M. (1983) Studies on chironomid midges of the Tama River. Part 5. An observation on the distribution of Chironominae along the main stream in June, with description of 15 new species. Research Report from the National Institute for Environmental Studies, 43, 1 - 67." type="journal article" year="1983">Sasa, 1983: 14</bibRefCitation>
</taxonomicName>
</treatmentCitation>
.
</treatmentCitationGroup>
</paragraph>
</subSubSection>
<subSubSection id="88C56558FFCEFFBDFF05FAEAFC8F6CCA" pageId="12" pageNumber="43" type="materials_examined">
<paragraph id="C06036D3FFCEFFBDFF05FAEAFC8F6CCA" blockId="12.[151,1437,1333,1683]" pageId="12" pageNumber="43">
<emphasis id="F2ABEAC1FFCEFFBDFF05FAEAFEFC6C39" bold="true" box="[151,377,1333,1359]" pageId="12" pageNumber="43">Material examined</emphasis>
.
<typeStatus id="1F648871FFCEFFBDFE14FAEAFE766C39" box="[390,499,1333,1359]" pageId="12" pageNumber="43" type="holotype">Holotype</typeStatus>
: Digital archive, National Museum of Nature and Science,
<collectingRegion id="021BF831FFCEFFBDFB13FAEAFB4E6C39" box="[1153,1227,1333,1359]" country="Japan" name="Tokyo" pageId="12" pageNumber="43">Tokyo</collectingRegion>
,
<collectingCountry id="B8C87643FFCEFFBDFB47FAE9FA9D6C26" box="[1237,1304,1334,1360]" name="Japan" pageId="12" pageNumber="43">Japan</collectingCountry>
;
<specimenCount id="D6D9FD5AFFCEFFBDFAB1FAEAFAFD6C39" box="[1315,1400,1333,1359]" pageId="12" pageNumber="43" type="male">3 males</specimenCount>
labelled
<taxonomicName id="07DF4D50FFCEFFBDFF79FA86FEFC6C05" box="[235,377,1369,1395]" class="Insecta" family="Chironomidae" genus="Polypedilum" kingdom="Animalia" order="Diptera" pageId="12" pageNumber="43" phylum="Arthropoda" rank="genus">
<emphasis id="F2ABEAC1FFCEFFBDFF79FA86FEFC6C05" box="[235,377,1369,1395]" italics="true" pageId="12" pageNumber="43">Polypedilum</emphasis>
</taxonomicName>
<taxonomicName id="07DF4D50FFCEFFBDFEECFA86FD3C6C05" authority="Sasa, 1983" authorityName="Sasa" authorityYear="1983" box="[382,697,1369,1396]" class="Insecta" family="Chironomidae" genus="Polypedilum" kingdom="Animalia" order="Diptera" pageId="12" pageNumber="43" phylum="Arthropoda" rank="species" species="tamanigrum" subGenus="Polypedilum">
(
<emphasis id="F2ABEAC1FFCEFFBDFE15FA85FE1D6C05" box="[391,408,1370,1395]" italics="true" pageId="12" pageNumber="43">P</emphasis>
.)
<emphasis id="F2ABEAC1FFCEFFBDFE3DFA85FDBD6C05" box="[431,568,1370,1395]" italics="true" pageId="12" pageNumber="43">tamanigrum</emphasis>
<bibRefCitation id="A44E4B22FFCEFFBDFDAFFA86FD3C6C05" author="Sasa, M." box="[573,697,1369,1395]" pageId="12" pageNumber="43" pagination="1 - 67" refId="ref37571" refString="Sasa, M. (1983) Studies on chironomid midges of the Tama River. Part 5. An observation on the distribution of Chironominae along the main stream in June, with description of 15 new species. Research Report from the National Institute for Environmental Studies, 43, 1 - 67." type="journal article" year="1983">Sasa, 1983</bibRefCitation>
</taxonomicName>
, West Bengal, Darjeeling, Takdah (27.03, 88.36),
<date id="B4611013FFCEFFBDFB72FA86FADF6C02" box="[1248,1370,1369,1396]" pageId="12" pageNumber="43" value="2019-06-05">05.vi.2019</date>
, Coll. D. Mondal. Retrieval and examination of illustrations of three types including
<typeStatus id="1F648871FFCEFFBDFB9CFAA2FBF56CE1" box="[1038,1136,1405,1431]" pageId="12" pageNumber="43" type="holotype">holotype</typeStatus>
from the digital archive of National Museum of Nature and Science,
<collectingRegion id="021BF831FFCEFFBDFDFFFA7EFD326CCD" box="[621,695,1441,1467]" country="Japan" name="Tokyo" pageId="12" pageNumber="43">Tokyo</collectingRegion>
,
<collectingCountry id="B8C87643FFCEFFBDFD50FA7DFC806CCA" box="[706,773,1442,1468]" name="Japan" pageId="12" pageNumber="43">Japan</collectingCountry>
.
</paragraph>
</subSubSection>
<subSubSection id="88C56558FFCEFFBDFF55FA1AFCB86FE5" pageId="12" pageNumber="43" type="discussion">
<paragraph id="C06036D3FFCEFFBDFF55FA1AFCB86FE5" blockId="12.[151,1437,1333,1683]" pageId="12" pageNumber="43">
<emphasis id="F2ABEAC1FFCEFFBDFF55FA1AFEB16CA9" bold="true" box="[199,308,1477,1503]" pageId="12" pageNumber="43">Remarks</emphasis>
(
<figureCitation id="58E42A56FFCEFFBDFED0FA1AFE216CA9" box="[322,420,1477,1503]" captionStart="FIGURES 9" captionStartId="18.[152,270,1879,1903]" captionTargetBox="[221,1360,195,1812]" captionTargetId="figure-25@18.[181,1406,178,1855]" captionTargetPageId="18" captionText="FIGURES 9AB. Polypedilum (Polypedilum) tamanigrum Sasa, 1983. A, Hypopygium (Photograph) of adult male. Scale: 0.1 mm; B, Neighbor-Joining tree based on the COX1 gene sequences of Polypedilum (Polypedilum) tamanigrum and 28 reference sequences of chironomine midges from GenBank. Red circles mark nodes. Scale: 0.01 substitutions per nucleotide position." figureDoi="http://doi.org/10.5281/zenodo.4397275" httpUri="https://zenodo.org/record/4397275/files/figure.png" pageId="12" pageNumber="43">Figure 9</figureCitation>
). Examination of three Indian specimens along with the
<typeStatus id="1F648871FFCEFFBDFB8EFA1AFBFB6CA9" box="[1052,1150,1477,1503]" pageId="12" pageNumber="43" type="holotype">holotype</typeStatus>
retrieved from the digital archive of National Museum of Nature and Science,
<collectingRegion id="021BF831FFCEFFBDFD4FFA36FCA26F75" box="[733,807,1513,1539]" country="Japan" name="Tokyo" pageId="12" pageNumber="43">Tokyo</collectingRegion>
,
<collectingCountry id="B8C87643FFCEFFBDFCA3FA35FCF46F72" box="[817,881,1514,1540]" name="Japan" pageId="12" pageNumber="43">Japan</collectingCountry>
and aligning the COX1 sequence in NCBI, it may be said that the present species fully conforms with those studied in this investigation with the following combination of characters: AR 0.650.68, DC 1214, Sct 810, biserial, superior volsella
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mm
</quantity>
long with middle outer lateral seta and two basal setae, and inferior volsella
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mm
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long with 8 apical setae. The sequence has showed ~1% divergence with MG949983.1 (
<figureCitation id="58E42A56FFCEFFBDFD28F9A6FCB46FE5" box="[698,817,1657,1683]" captionStart="FIGURES 9" captionStartId="18.[152,270,1879,1903]" captionTargetBox="[221,1360,195,1812]" captionTargetId="figure-25@18.[181,1406,178,1855]" captionTargetPageId="18" captionText="FIGURES 9AB. Polypedilum (Polypedilum) tamanigrum Sasa, 1983. A, Hypopygium (Photograph) of adult male. Scale: 0.1 mm; B, Neighbor-Joining tree based on the COX1 gene sequences of Polypedilum (Polypedilum) tamanigrum and 28 reference sequences of chironomine midges from GenBank. Red circles mark nodes. Scale: 0.01 substitutions per nucleotide position." figureDoi="http://doi.org/10.5281/zenodo.4397275" httpUri="https://zenodo.org/record/4397275/files/figure.png" pageId="12" pageNumber="43">Figure 9B</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
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