91 lines
9.8 KiB
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91 lines
9.8 KiB
XML
<document id="126D6AA82F22FF721081BF48235C0326" ID-DOI="10.1016/j.phytochem.2018.10.027" ID-ISSN="1873-3700" ID-Zenodo-Dep="10481193" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="karina" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="karina" IM.treatments_approvedBy="karina" checkinTime="1704925253971" checkinUser="felipe" docAuthor="Andersen, Trine Bundgaard, Rasmussen, Silas Anselm, Christensen, Søren Brøgger & Simonsen, Henrik Toft" docDate="2019" docId="039087C3FF984065FFC073CEE449D9F8" docLanguage="en" docName="Phytochemistry.157.168-174.pdf" docOrigin="Phytochemistry 157" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.10.027" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Nicotiana benthamiana" docType="treatment" docVersion="5" lastPageNumber="170" masterDocId="FFA9FFBBFF9A4067FFA47619E55DDE50" masterDocTitle="Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy" masterLastPageNumber="174" masterPageNumber="168" pageNumber="170" updateTime="1706402960486" updateUser="ExternalLinkService">
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<mods:titleInfo id="232305A53235363AC592AE23F3BB1240">
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<mods:title id="9CF9CF5899613F86033841C3DA41E102">Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy</mods:title>
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<mods:name id="D024DC3C8A7F2452284390580EB24160" type="personal">
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<mods:roleTerm id="072DCFD8335E33F993B74F5864BBBB45">Author</mods:roleTerm>
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<mods:namePart id="E2AFB27F3A3757D4DDC0BF823A465B44">Andersen, Trine Bundgaard</mods:namePart>
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<mods:affiliation id="E780F05F15998AD6D07980297DAD09CA">Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark</mods:affiliation>
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<mods:namePart id="FD845C09A0D278A3731F2BFBB68AD3BF">Rasmussen, Silas Anselm</mods:namePart>
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<mods:namePart id="E55AE3AC0CAECCA9F4C18BF96E8141E5">Christensen, Søren Brøgger</mods:namePart>
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<mods:namePart id="9B426CD357CE8E9934053D047D61F6C1">Simonsen, Henrik Toft</mods:namePart>
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<mods:typeOfResource id="CBDA2532AB59C57E146C4495EA3A7DE3">text</mods:typeOfResource>
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<mods:title id="FC7988C931E81F521637D36D66291A49">Phytochemistry</mods:title>
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<mods:part id="D7DE6DE692B05F58333BBD474C595F57">
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<mods:date id="0831CA284525AF7094588BDFA8FE9E63">2019</mods:date>
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<mods:number id="BC25F83893C68A55A0D4B6BF34A21074">2019-01-31</mods:number>
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<mods:number id="757757866A2F4A81B0D4C527805CDFFA">157</mods:number>
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<mods:start id="153BA7CBDC3A783BA00D067A2FF8E0D0">168</mods:start>
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<mods:classification id="B96EA5D1F21E421E5A5B07D9E256CF10">journal article</mods:classification>
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<mods:identifier id="FC6C1177BBCD86641715EA0E2C26D7B1" type="DOI">10.1016/j.phytochem.2018.10.027</mods:identifier>
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<mods:identifier id="CC8FF9DAB7FB8CB77DCBF4934E036729" type="ISSN">1873-3700</mods:identifier>
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<mods:identifier id="E2CF7F46B849113680D4CA4373738B46" type="Zenodo-Dep">10481193</mods:identifier>
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<treatment id="039087C3FF984065FFC073CEE449D9F8" ID-DOI="http://doi.org/10.5281/zenodo.10576567" ID-Zenodo-Dep="10576567" LSID="urn:lsid:plazi:treatment:039087C3FF984065FFC073CEE449D9F8" httpUri="http://treatment.plazi.org/id/039087C3FF984065FFC073CEE449D9F8" lastPageNumber="170" pageId="2" pageNumber="170">
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<subSubSection id="C323655EFF984065FFC073CEE79DDBBA" box="[100,704,1495,1514]" pageId="2" pageNumber="170" type="nomenclature">
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<paragraph id="8B8636D5FF984065FFC073CEE79DDBBA" blockId="2.[100,704,1495,1514]" box="[100,704,1495,1514]" pageId="2" pageNumber="170">
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<heading id="D0CE81B9FF984065FFC073CEE79DDBBA" bold="true" box="[100,704,1495,1514]" fontSize="36" level="1" pageId="2" pageNumber="170" reason="1">
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<emphasis id="B94DEAC7FF984065FFC073CEE79DDBBA" bold="true" box="[100,704,1495,1514]" italics="true" pageId="2" pageNumber="170">
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2.4. Characterization of TlTPS18983 expressed in
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<taxonomicName id="4C394D56FF984065FD9573CEE79DDBBA" ID-CoL="47D27" authority="Domin" box="[561,704,1495,1514]" class="Magnoliopsida" family="Solanaceae" genus="Nicotiana" kingdom="Plantae" order="Solanales" pageId="2" pageNumber="170" phylum="Tracheophyta" rank="species" species="benthamiana">N. benthamiana</taxonomicName>
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C323655EFF984065FF217017E449D9F8" pageId="2" pageNumber="170" type="description">
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<paragraph id="8B8636D5FF984065FF217017E449D9F8" blockId="2.[100,770,1550,1960]" pageId="2" pageNumber="170">
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Volatiles from
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<taxonomicName id="4C394D56FF984065FEB17017E4F8D871" box="[277,421,1550,1569]" class="Magnoliopsida" family="Solanaceae" genus="Nicotiana" kingdom="Plantae" order="Solanales" pageId="2" pageNumber="170" phylum="Tracheophyta" rank="species" species="benthamiana">
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<emphasis id="B94DEAC7FF984065FEB17017E4F8D871" bold="true" box="[277,421,1550,1569]" italics="true" pageId="2" pageNumber="170">N. benthamiana</emphasis>
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</taxonomicName>
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leaves expressing
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<emphasis id="B94DEAC7FF984065FDFE7017E731D871" bold="true" box="[602,620,1550,1569]" italics="true" pageId="2" pageNumber="170">Tl</emphasis>
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TPS18983 were captured by HS-SPME and analyzed by GC-MS. The analysis of these plants showed that expression of
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<emphasis id="B94DEAC7FF984065FE1E705FE491D809" bold="true" box="[442,460,1606,1625]" italics="true" pageId="2" pageNumber="170">Tl</emphasis>
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TPS18983 generate at least 15 products. The identification of one of the major products, farnesene (
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<emphasis id="B94DEAC7FF984065FD43707BE7AED825" bold="true" box="[743,755,1634,1653]" pageId="2" pageNumber="170">5</emphasis>
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), was based on comparison of the mass spectrum of the compound with NIST and Wiley GC-MS spectrum libraries (
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<figureCitation id="13022A50FF984065FDB87083E70AD8FD" box="[540,599,1690,1709]" captionStart="Fig" captionStartId="3.[100,130,830,847]" captionTargetBox="[264,1324,156,803]" captionTargetId="figure-593@3.[263,1325,152,807]" captionTargetPageId="3" captionText="Fig. 4. a) HS-SPME GC-MS analysis of TlTPS18983 expressed together AtHMGR, the control sample show the expression of AtHMGR alone. Farnesene (5) is seen as the major product (Rt 8.75 min), * show unidentified sesquiterpene like products. Based on this TlTPS18983 is a multiproduct terpene synthase with farnesene as the major product. MS spectra are presented in supplementary data." figureDoi="http://doi.org/10.5281/zenodo.10481201" httpUri="https://zenodo.org/record/10481201/files/figure.png" pageId="2" pageNumber="170">Fig. 4</figureCitation>
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, MS spectrum is provided in supplementary data
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<figureCitation id="13022A50FF984065FE3F70AFE48CD899" box="[411,465,1718,1737]" captionStart="Fig" captionStartId="2.[100,130,825,842]" captionTargetBox="[264,1322,155,800]" captionTargetId="figure-761@2.[263,1325,152,802]" captionTargetPageId="2" captionText="Fig. 3. HS-SPME GC-MS analysis of TlTPS509 expressed together AtHMGR, the control shows the expression of AtHMGR alone. Guaiol (3) is seen as the major product (Rt 9.89 min), with bulnesol (4), Rt: 10.2, a-guaiene (Rt: 8.6), and a-bulnesene (Rt: 9.18) as minor products. * show unidentified sesquiterpene like products, which is either ketone formed during the biosynthesis or breakdown products of the alcohols formed during the GC-MS analysis. Based on this TlTPS509 is a guaiol synthase. MS spectra are presented in supplementary data." figureDoi="http://doi.org/10.5281/zenodo.10481199" httpUri="https://zenodo.org/record/10481199/files/figure.png" pageId="2" pageNumber="170">Fig. 3</figureCitation>
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). It was not possible to identify the remaining products. Using an injection port temperature of 160 ̊C instead of 250 ̊C did not alter the composition of the chromatogram, which supports that these compounds are not a result of the GC-MS analysis method. Extraction of the products was also attempted using organic solvents such as hexane and pentane mimicking procedures that were successful for other sqTPS products. However, for
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<emphasis id="B94DEAC7FF984065FFC07160E52BD9DC" bold="true" box="[100,118,1913,1932]" italics="true" pageId="2" pageNumber="170">Tl</emphasis>
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TPS18983 we did not detect any compounds by GC-MS using organic solvent extraction.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |