148 lines
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148 lines
14 KiB
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<document id="B3959BAE4E447D5B192975B4669C9EF2" ID-DOI="10.1016/j.phytochem.2020.112283" ID-ISSN="1873-3700" ID-Zenodo-Dep="8294206" IM.bibliography_approvedBy="diego" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="diego" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="diego" IM.treatments_approvedBy="diego" checkinTime="1693255514846" checkinUser="felipe" docAuthor="Lu, Xiaoxiao, Xie, Chunqin, Wang, Yunxiao, Liu, Yongnan, Han, Jing, Shi, Liang, Zhu, Jing, Yu, Hanshou, Ren, Ang & Zhao, Mingwen" docDate="2020" docId="03D26736FF9FFF89116CDC9BFE8EF890" docLanguage="en" docName="Phytochemistry.172.112283.pdf" docOrigin="Phytochemistry (112283) 172" docSource="http://dx.doi.org/10.1016/j.phytochem.2020.112283" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Ganoderma lucidum subsp. contains P. Karst." docType="treatment" docVersion="3" lastPageNumber="7" masterDocId="FFEB1F4EFF99FF8F1108D833FFF6FF94" masterDocTitle="Function of ceramide synthases on growth, ganoderic acid biosynthesis and sphingolipid homeostasis in Ganoderma lucidum" masterLastPageNumber="9" masterPageNumber="1" pageNumber="7" updateTime="1693401629264" updateUser="diego">
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<mods:title id="838CB6CB975BC1D0E1A4829B4C63006B">Function of ceramide synthases on growth, ganoderic acid biosynthesis and sphingolipid homeostasis in Ganoderma lucidum</mods:title>
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<mods:namePart id="A403C3D353C0FFB76A319EC1548FF1D1">Lu, Xiaoxiao</mods:namePart>
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<mods:affiliation id="EE810592501FC04A36FC582BBEFD1823">∗ & Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing</mods:affiliation>
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<mods:namePart id="31BE933BE08049AE5552401305B0FC05">Xie, Chunqin</mods:namePart>
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<mods:namePart id="D8AA2670999CFC0969E2EE46081A1AFD">Wang, Yunxiao</mods:namePart>
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<mods:namePart id="B305529F2CC3EFD281D942E82B33722A">Liu, Yongnan</mods:namePart>
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<mods:namePart id="4E502CCC2AE4AEA020963420A750BEC0">Han, Jing</mods:namePart>
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<mods:namePart id="412E7049C3C087749F264475C86B8CEE">Shi, Liang</mods:namePart>
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<mods:namePart id="CFC88871061AD00074CC2E29BF7D03C7">Zhu, Jing</mods:namePart>
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<mods:namePart id="692DFF5F20EE92F1E87F439184CDEE30">Yu, Hanshou</mods:namePart>
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<mods:namePart id="7DB8D1AE259D2B3EAB6D046E4B4180C8">Ren, Ang</mods:namePart>
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<mods:namePart id="2E6B272CCB234C6BDC80684B04B15CCE">Zhao, Mingwen</mods:namePart>
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<mods:title id="5558F098B249F7D9F4DA5DD3773A5013">Phytochemistry</mods:title>
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<mods:date id="E318C37FFD9520C37C1D873BA7209919">2020</mods:date>
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<mods:number id="32F8ABB784753F89F30CAE1FA674F061">2020-04-30</mods:number>
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<mods:number id="ACB4844979B6B2F66D27FB553C463CD5">172</mods:number>
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<treatment id="03D26736FF9FFF89116CDC9BFE8EF890" LSID="urn:lsid:plazi:treatment:03D26736FF9FFF89116CDC9BFE8EF890" httpUri="http://treatment.plazi.org/id/03D26736FF9FFF89116CDC9BFE8EF890" lastPageNumber="7" pageId="6" pageNumber="7">
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<subSubSection id="C36185ABFF9FFF89116CDC9BFD7BFB2F" box="[100,653,1192,1211]" pageId="6" pageNumber="7" type="nomenclature">
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<paragraph id="8BC4D620FF9FFF89116CDC9BFD7BFB2F" blockId="6.[100,653,1192,1211]" box="[100,653,1192,1211]" pageId="6" pageNumber="7">
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<heading id="D08C614CFF9FFF89116CDC9BFD7BFB2F" bold="true" box="[100,653,1192,1211]" fontSize="36" level="1" pageId="6" pageNumber="7" reason="1">
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<emphasis id="B90F0A32FF9FFF89116CDC9BFD7BFB2F" bold="true" box="[100,653,1192,1211]" italics="true" pageId="6" pageNumber="7">
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5.2. Gene cloning and sequence analysis of lag in
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<taxonomicName id="4C7BADA3FF9FFF891322DC9BFD7BFB2F" ID-CoL="6JWYZ" ID-ENA="5315" authority="(Curtis) P. Karst." authorityName="P. Karst." baseAuthorityName="Curtis" box="[554,653,1192,1211]" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="6" pageNumber="7" phylum="Basidiomycota" rank="species" species="lucidum">G. lucidum</taxonomicName>
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</emphasis>
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<paragraph id="8BC4D620FF9FFF89118DDCECFECFF9D5" blockId="6.[100,771,1247,1796]" pageId="6" pageNumber="7">
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The sequences of CERSs from
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<taxonomicName id="4C7BADA3FF9FFF891092DCECFDC8FB66" authority=", LAG" authorityName="LAG" box="[410,574,1247,1266]" class="Tremellomycetes" family="Tremellaceae" genus="Saccharomyces" kingdom="Fungi" order="Tremellales" pageId="6" pageNumber="7" phylum="Basidiomycota" rank="species" species="cerevisiae">
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<emphasis id="B90F0A32FF9FFF891092DCECFDF3FB66" bold="true" box="[410,517,1247,1266]" italics="true" pageId="6" pageNumber="7">S. cerevisiae</emphasis>
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, LAG
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</taxonomicName>
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1 and LAC1 (National Center for Biotechnology Information (NCBI), GenBank accession number: NP_011860.1 and NP_012917.3), were used for a homology search of the genome database of
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<taxonomicName id="4C7BADA3FF9FFF8910BDDD00FDECFAD2" box="[437,538,1331,1350]" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="6" pageNumber="7" phylum="Basidiomycota" rank="species" species="lucidum">
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<emphasis id="B90F0A32FF9FFF8910BDDD00FDECFAD2" bold="true" box="[437,538,1331,1350]" italics="true" pageId="6" pageNumber="7">G. lucidum</emphasis>
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</taxonomicName>
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strain 2,601,251 (NCBI taxid: 1,077,286) (
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<bibRefCitation id="EFEAABD1FF9FFF891010DD7CFE4AFAF6" author="Chen, S. L. & Xu, J. & Liu, C. & Zhu, Y. J. & Nelson, D. R. & Zhou, S. G. & Li, C. F. & Wang, L. Z. & Guo, X. & Sun, Y. Z. & Luo, H. M. & Li, Y. & Song, J. Y. & Henrissat, B. & Levasseur, A. & Qian, J. & Li, J. Q. & Luo, X. & Shi, L. C. & He, L. & Xiang, L. & Xu, X. L. & Niu, Y. Y. & Li, Q. S. & Han, M. V. & Yan, H. X. & Zhang, J. & Chen, H. M. & Wang, Z. & Liu, M. Z. & Schwartz, D. C. & Sun, C." box="[280,444,1359,1378]" pageId="6" pageNumber="7" refId="ref8664" refString="Chen, S. L., Xu, J., Liu, C., Zhu, Y. J., Nelson, D. R., Zhou, S. G., Li, C. F., Wang, L. Z., Guo, X., Sun, Y. Z., Luo, H. M., Li, Y., Song, J. Y., Henrissat, B., Levasseur, A., Qian, J., Li, J. Q., Luo, X., Shi, L. C., He, L., Xiang, L., Xu, X. L., Niu, Y. Y., Li, Q. S., Han, M. V., Yan, H. X., Zhang, J., Chen, H. M., Lv, A. P., Wang, Z., Liu, M. Z., Schwartz, D. C., Sun, C., 2012. Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nat. Commun. 3." type="book" year="2012">Chen et al., 2012</bibRefCitation>
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). Three genes (
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<emphasis id="B90F0A32FF9FFF89135ADD7CFD81FAF6" bold="true" box="[594,631,1359,1378]" italics="true" pageId="6" pageNumber="7">lag1</emphasis>
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,
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<emphasis id="B90F0A32FF9FFF89138DDD7CFD5CFAF6" bold="true" box="[645,682,1359,1378]" italics="true" pageId="6" pageNumber="7">lag2</emphasis>
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and
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<emphasis id="B90F0A32FF9FFF8913D4DD7CFCF4FAF6" bold="true" box="[732,770,1359,1378]" italics="true" pageId="6" pageNumber="7">lag3</emphasis>
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with the GenBank accession numbers MH145349, MH145350 and MH145351, respectively) were identified as genes encoding CERS homologues. Using the primers described in Supplementary Table S1, the three
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<emphasis id="B90F0A32FF9FFF8911CBDD8DFF2BFA45" bold="true" box="[195,221,1470,1489]" italics="true" pageId="6" pageNumber="7">lag</emphasis>
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coding sequences were polymerase chain reaction (PCR) amplified from complementary DNA (cDNA) and sequenced. From comparisons between the cDNA and genomic sequences, the ORFs (open reading frames) and exon/intron positions of three
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<emphasis id="B90F0A32FF9FFF8913ACDE21FD48F9B1" bold="true" box="[676,702,1554,1573]" italics="true" pageId="6" pageNumber="7">lag</emphasis>
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s in
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<taxonomicName id="4C7BADA3FF9FFF8913E5DE21FF5DF9D5" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="6" pageNumber="7" phylum="Basidiomycota" rank="species" species="lucidum">
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<emphasis id="B90F0A32FF9FFF8913E5DE21FF5DF9D5" bold="true" italics="true" pageId="6" pageNumber="7">G. lucidum</emphasis>
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</taxonomicName>
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were inferred.
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</paragraph>
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<paragraph id="8BC4D620FF9FFF89118DDE79FE8EF890" blockId="6.[100,771,1247,1796]" pageId="6" pageNumber="7">
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The conserved domains were identified by SMART (http://smart. emblheidelberg.de) (
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<bibRefCitation id="EFEAABD1FF9FFF891039DE55FDF7F9ED" author="Letunic, I. & Doerks, T. & Bork, P." box="[305,513,1638,1657]" pageId="6" pageNumber="7" refId="ref9473" refString="Letunic, I., Doerks, T., Bork, P., 2009. Smart 6: recent updates and new developments. Nucleic Acids Res. 37, D 229 - D 232." type="journal volume" year="2009">Letunic et al., 2009</bibRefCitation>
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). The multiple sequence alignments were obtained with T-COFFEE (http://www.tcoffee.org) (
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<bibRefCitation id="EFEAABD1FF9FFF891164DEADFEFFF925" author="Taly, J. F. & Magis, C. & Bussotti, G. & Chang, J. M. & Di Tommaso, P. & Erb, I. & Espinosa-Carrasco, J. & Kemena, C. & Notredame, C." box="[108,265,1694,1713]" pageId="6" pageNumber="7" pagination="1669 - 1682" refId="ref10733" refString="Taly, J. F., Magis, C., Bussotti, G., Chang, J. M., Di Tommaso, P., Erb, I., Espinosa-Carrasco, J., Kemena, C., Notredame, C., 2011. Using the T-Coffee package to build multiple sequence alignments of protein, RNA, DNA sequences and 3 D structures. Nat. Protoc. 6, 1669 - 1682." type="journal article" year="2011">Taly et al., 2011</bibRefCitation>
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). The phylogenetic tree was constructed through the application of MEGA 6.0 program (
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<bibRefCitation id="EFEAABD1FF9FFF8910DADE8AFD56F958" author="Tamura, K. & Stecher, G. & Peterson, D. & Filipski, A. & Kumar, S." box="[466,672,1721,1740]" pageId="6" pageNumber="7" pagination="2725 - 2729" refId="ref10816" refString="Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., 2013. MEGA 6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725 - 2729." type="journal article" year="2013">Tamura et al., 2013</bibRefCitation>
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) and the neighbour-joining method with 1000 bootstrap replicates was applied to infer evolutionary history.
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</paragraph>
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</subSubSection>
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</treatment>
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