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<document id="49126497B15757A10BEFC462FC2403DD" ID-CLB-Dataset="20559" ID-DOI="10.1099/ijsem.0.004344" ID-GBIF-Dataset="5674f540-5072-4695-8698-4373cc982fcd" ID-ISSN="1466-5034" ID-Zenodo-Dep="6224040" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1645466022237" checkinUser="felipe" docAuthor="Siebert, Annemarie, Huptas, Christopher, Wenning, Mareike, Scherer, Siegfried &amp; Doll, Etienne V." docDate="2020" docId="03AE3052FF8AFFD3FFB0665223BFF845" docLanguage="en" docName="IntJSystEvolMicrobiol.70.8.4774-4781.pdf" docOrigin="International Journal of Systematic and Evolutionary Microbiology 70 (8)" docSource="http://dx.doi.org/10.1099/ijsem.0.004344" docStyle="DocumentStyle:C64F0A4F4C66F6FC2AD4ED89351C6242.3:IntJSystEvolMicrobiol.2017-.journal_article" docStyleId="C64F0A4F4C66F6FC2AD4ED89351C6242" docStyleName="IntJSystEvolMicrobiol.2017-.journal_article" docStyleVersion="3" docTitle="Fundicoccus IGNAVUS 2020, GEN. NOV., SP. NOV." docType="treatment" docVersion="9" lastPageNumber="4780" masterDocId="FF97482AFF8CFFD5FFC96468213DFFE6" masterDocTitle="Fundicoccus ignavus gen. nov., sp. nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk" masterLastPageNumber="4781" masterPageNumber="4774" pageNumber="4780" updateTime="1699315863245" updateUser="ExternalLinkService">
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<mods:title id="AA8BC8030061AEA322F9F23E969A7D26">Fundicoccus ignavus gen. nov., sp. nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk</mods:title>
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<mods:namePart id="5FE46747C4142ADB82ABB7726A896DC2">Siebert, Annemarie</mods:namePart>
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<mods:namePart id="3EB974CEBB16B95DEB144FFEA0CCB482">Huptas, Christopher</mods:namePart>
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<mods:namePart id="C8205232870589B228CE84D772CE1735">Wenning, Mareike</mods:namePart>
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<mods:namePart id="974DC2B980F9C2132F591BA97214BF50">Scherer, Siegfried</mods:namePart>
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<mods:namePart id="361086177DBDED6D6F5991B053E0670B">Doll, Etienne V.</mods:namePart>
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<mods:title id="06F4BD09B9C0B44952BD0264A93CAC31">International Journal of Systematic and Evolutionary Microbiology</mods:title>
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<mods:date id="38EBAD4710DA474768226403ED2077BC">2020</mods:date>
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<treatment id="03AE3052FF8AFFD3FFB0665223BFF845" ID-DOI="http://doi.org/10.5281/zenodo.6314501" ID-GBIF-Taxon="193422057" ID-Zenodo-Dep="6314501" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845" httpUri="http://treatment.plazi.org/id/03AE3052FF8AFFD3FFB0665223BFF845" lastPageNumber="4780" pageId="6" pageNumber="4780">
<subSubSection id="C31DD2CFFF8AFFD3FFB0665220B6FD9A" pageId="6" pageNumber="4780" type="nomenclature">
<paragraph id="8BB88144FF8AFFD3FFB0665220B6FD9A" blockId="6.[115,763,570,1955]" pageId="6" pageNumber="4780">
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<emphasis id="B9735D56FF8AFFD3FFB0665220B6FD9A" bold="true" pageId="6" pageNumber="4780">
DESCRIPTION OF
<taxonomicName id="4C07FAC7FF8AFFD3FEBB66522383FDB1" authority="IGNAVUS" authorityName="IGNAVUS" authorityYear="2020" box="[370,702,570,599]" class="Bacilli" family="Aerococcaceae" genus="Fundicoccus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="6" pageNumber="4780" phylum="Firmicutes" rank="genus" status="gen. nov.">
<emphasis id="B9735D56FF8AFFD3FEBB66522383FDB1" bold="true" box="[370,702,570,599]" italics="true" pageId="6" pageNumber="4780">FUNDICOCCUS IGNAVUS</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A240E02DFF8AFFD3FFB0663720B6FD9A" box="[121,395,607,636]" pageId="6" pageNumber="4780" rank="species">GEN. NOV., SP. NOV.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
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<subSubSection id="C31DD2CFFF8AFFD3FFB066E123CCFD26" pageId="6" pageNumber="4780" type="etymology">
<paragraph id="8BB88144FF8AFFD3FFB066E123CCFD26" blockId="6.[115,763,570,1955]" pageId="6" pageNumber="4780">
<taxonomicName id="4C07FAC7FF8AFFD3FFB066E12073FD47" authorityName="Siebert &amp; Huptas &amp; Wenning &amp; Scherer &amp; Doll" authorityYear="2020" box="[121,334,649,673]" class="Bacilli" family="Aerococcaceae" genus="Fundicoccus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="6" pageNumber="4780" phylum="Firmicutes" rank="species" species="ignavus">
<emphasis id="B9735D56FF8AFFD3FFB066E12073FD47" box="[121,334,649,673]" italics="true" pageId="6" pageNumber="4780">Fundicoccus ignavus</emphasis>
</taxonomicName>
(ig.navus. L. masc. adj.
<taxonomicName id="4C07FAC7FF8AFFD3FD8066E223A4FD47" authorityName="Siebert &amp; Huptas &amp; Wenning &amp; Scherer &amp; Doll" authorityYear="2020" box="[585,665,650,673]" class="Bacilli" family="Aerococcaceae" genus="Fundicoccus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="6" pageNumber="4780" phylum="Firmicutes" rank="species" species="ignavus">
<emphasis id="B9735D56FF8AFFD3FD8066E223A4FD47" box="[585,665,650,673]" italics="true" pageId="6" pageNumber="4780">ignavus</emphasis>
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inactive, referring to the lack of acid formation from carbohydrates).
</paragraph>
</subSubSection>
<subSubSection id="C31DD2CFFF8AFFD3FFB066BE204EF99B" pageId="6" pageNumber="4780" type="description">
<paragraph id="8BB88144FF8AFFD3FFB066BE2358FA14" blockId="6.[115,763,570,1955]" pageId="6" pageNumber="4780">
<location id="8ED8D79FFF8AFFD3FFB066BE2190FD08" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FFB066BE2190FD08" box="[121,173,726,750]" country="Switzerland" county="The" municipality="Additionally" name="Cells" pageId="6" pageNumber="4780">Cells</location>
are circular-shaped (cocci) with an approximate size of 12µm.
<location id="8ED8D79FFF8AFFD3FF07669D203CFCEB" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FF07669D203CFCEB" box="[206,257,757,781]" country="Switzerland" county="The" municipality="Additionally" name="They" pageId="6" pageNumber="4780">They</location>
occur as single cells and
<taxonomicName id="4C07FAC7FF8AFFD3FE3D669D2360FCEB" box="[500,605,757,781]" form="pairs" pageId="6" pageNumber="4780" rank="form">form pairs</taxonomicName>
or chains.
<location id="8ED8D79FFF8AFFD3FD0C669D23C5FCEB" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FD0C669D23C5FCEB" box="[709,760,757,781]" country="Switzerland" county="The" municipality="Additionally" name="Cells" pageId="6" pageNumber="4780">Cells</location>
are Gram-stain-positive, non-motile, non-endospore-forming, oxidase- and catalase-negative, and facultatively anaerobic.
<location id="8ED8D79FFF8AFFD3FD19675B23C5FCAD" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FD19675B23C5FCAD" box="[720,760,819,843]" country="Switzerland" county="The" municipality="Additionally" name="The" pageId="6" pageNumber="4780">The</location>
isolates show strong α- haemolysis on 5% sheep blood agar.
<location id="8ED8D79FFF8AFFD3FD19673923C5FC8F" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FD19673923C5FC8F" box="[720,760,849,873]" country="Switzerland" county="The" municipality="Additionally" name="The" pageId="6" pageNumber="4780">The</location>
optimal growth conditions are at 2933 °C, pH 7.58.0 and 0.51% (w/v) NaCl. Growth occurs at 1239 °C with one strain only growing up to 37 °C (WS5303).
<location id="8ED8D79FFF8AFFD3FE2267C6230FFC20" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FE2267C6230FFC20" box="[491,562,942,966]" country="Switzerland" county="The" municipality="Additionally" name="Strains" pageId="6" pageNumber="4780">Strains</location>
grow at pH 6.59.5 and strain WS4937
<superScript id="7C722C0CFF8AFFD3FEF767A42075FC3C" attach="left" box="[318,328,972,986]" fontSize="6" pageId="6" pageNumber="4780">T</superScript>
as well as WS4753 are able to grow in the presence of up to 6.5% (w/v) NaCl. Acid formation was not detected for any of the 47 carbohydrates tested with the API 50
<collectingCountry id="F310C1D4FF8AFFD3FF5060422182FBA4" box="[153,191,1066,1090]" name="Switzerland" pageId="6" pageNumber="4780">CH</collectingCountry>
system.
<location id="8ED8D79FFF8AFFD3FEDE60422070FBA7" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FEDE60422070FBA7" box="[279,333,1066,1089]" country="Switzerland" county="The" municipality="Additionally" name="Does" pageId="6" pageNumber="4780">Does</location>
not hydrolyse starch, gelatine or aesculin.
<location id="8ED8D79FFF8AFFD3FFB0602121EEFB87" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FFB0602121EEFB87" box="[121,211,1097,1121]" country="Switzerland" county="The" municipality="Additionally" name="Negative" pageId="6" pageNumber="4780">Negative</location>
for the
<location id="8ED8D79FFF8AFFD3FEEC602120E5FB87" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FEEC602120E5FB87" box="[293,472,1097,1121]" country="Switzerland" county="The" municipality="Additionally" name="Voges - Proskauer" pageId="6" pageNumber="4780">VogesProskauer</location>
test and indole production.
<location id="8ED8D79FFF8AFFD3FFB3600021FFFB66" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FFB3600021FFFB66" box="[122,194,1128,1152]" country="Switzerland" county="The" municipality="Additionally" name="Nitrate" pageId="6" pageNumber="4780">Nitrate</location>
is not reduced. H
<subScript id="17838301FF8AFFD3FEB860102045FB60" attach="right" box="[369,376,1144,1158]" fontSize="6" pageId="6" pageNumber="4780">2</subScript>
S is not detected.
<location id="8ED8D79FFF8AFFD3FDED60002349FB66" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FDED60002349FB66" box="[548,628,1128,1152]" country="Switzerland" county="The" municipality="Additionally" name="Positive" pageId="6" pageNumber="4780">Positive</location>
for argininedihdyrolase,
<emphasis id="B9735D56FF8AFFD3FF3460EF2037FB79" box="[253,266,1159,1183]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-galactosidase activity (only with resorufin
<emphasis id="B9735D56FF8AFFD3FD0060EF23EBFB79" box="[713,726,1159,1183]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-Dgalactopyranoside as substrate) and hydrolysis of hippurate. Alanyl-phenylalanyl-proline-arylamidase, alkaline phosphatase,
<emphasis id="B9735D56FF8AFFD3FF1D608B21DCFB1D" box="[212,225,1251,1275]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-glucuronidase,
<emphasis id="B9735D56FF8AFFD3FE40608B20ABFB1D" box="[393,406,1251,1275]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-mannosidase, glycyl-tryptophanarylamidase,
<emphasis id="B9735D56FF8AFFD3FECC616B2025FAFC" box="[261,280,1283,1306]" italics="true" pageId="6" pageNumber="4780">N</emphasis>
-acetyl-
<emphasis id="B9735D56FF8AFFD3FEAF616A204EFAFC" box="[358,371,1282,1306]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-glucosaminidase, pyroglutamic acid arylamidase and urease are not produced.
<emphasis id="B9735D56FF8AFFD3FD80614A2368FADF" box="[585,597,1314,1337]" italics="true" pageId="6" pageNumber="4780">α</emphasis>
-
<location id="8ED8D79FFF8AFFD3FD96614923E3FADF" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FD96614923E3FADF" box="[607,734,1313,1337]" country="Switzerland" county="The" municipality="Additionally" name="Glucosidase" pageId="6" pageNumber="4780">Glucosidase</location>
is weakly positive for WS4937
<superScript id="7C722C0CFF8AFFD3FE566157209AFAAB" attach="right" box="[415,423,1343,1357]" fontSize="6" pageId="6" pageNumber="4780">T</superScript>
.
<location id="8ED8D79FFF8AFFD3FE7C612820E0FABE" LSID="urn:lsid:plazi:treatment:03AE3052FF8AFFD3FFB0665223BFF845:8ED8D79FFF8AFFD3FE7C612820E0FABE" box="[437,477,1344,1368]" country="Switzerland" county="The" municipality="Additionally" name="The" pageId="6" pageNumber="4780">The</location>
phenotype for amino acid catabolism was supported by genome data.
<collectingMunicipality id="6BDC1B3EFF8AFFD3FD99613723E9FA91" box="[592,724,1375,1399]" pageId="6" pageNumber="4780">Additionally</collectingMunicipality>
, an orthologue of the alkaline phosphatase was detected which is not active under conditions used in phenotypic experiments.
<collectingCounty id="62D9F9C8FF8AFFD3FFB261D4219EFA32" box="[123,163,1468,1492]" pageId="6" pageNumber="4780">The</collectingCounty>
production of
<emphasis id="B9735D56FF8AFFD3FE8B61D42073FA32" box="[322,334,1468,1492]" italics="true" pageId="6" pageNumber="4780">β</emphasis>
-glucosidase is variable but was detected for the
<typeStatus id="54BC3FE6FF8AFFD3FF0D61B321CCFA15" box="[196,241,1499,1523]" pageId="6" pageNumber="4780">type</typeStatus>
strain WS4937
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and strain WS5303.
</paragraph>
<paragraph id="8BB88144FF8AFFD3FFB06261204EF99B" blockId="6.[115,763,570,1955]" pageId="6" pageNumber="4780">
The major fatty acids are C
<subScript id="17838301FF8AFFD3FE63627120FBF9C1" attach="both" box="[426,454,1561,1575]" fontSize="6" pageId="6" pageNumber="4780">16:1</subScript>
ω9
<emphasis id="B9735D56FF8AFFD3FE2C626120D2F9C6" box="[485,495,1545,1568]" italics="true" pageId="6" pageNumber="4780">с</emphasis>
, C
<subScript id="17838301FF8AFFD3FDD862712310F9C1" attach="left" box="[529,557,1561,1575]" fontSize="6" pageId="6" pageNumber="4780">16:0</subScript>
and C
<subScript id="17838301FF8AFFD3FDB3627123ABF9C1" attach="both" box="[634,662,1561,1575]" fontSize="6" pageId="6" pageNumber="4780">18:1</subScript>
ω9
<emphasis id="B9735D56FF8AFFD3FD7C62612382F9C6" box="[693,703,1545,1568]" italics="true" pageId="6" pageNumber="4780">с</emphasis>
. The polar lipid profile consists of three glycolipids, phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol.
</paragraph>
</subSubSection>
<subSubSection id="C31DD2CFFF8AFFD3FFB062FB23BFF845" pageId="6" pageNumber="4780" type="materials_examined">
<paragraph id="8BB88144FF8AFFD3FFB062FB23BFF845" blockId="6.[115,763,570,1955]" pageId="6" pageNumber="4780">
<materialsCitation id="3B6F8B19FF8AFFD3FFB062FB23BFF845" ID-GBIF-Occurrence="3470324301" collectionCode="WS" pageId="6" pageNumber="4780" specimenCode="WS4937T" specimenCount="1" typeStatus="holotype">
The
<typeStatus id="54BC3FE6FF8AFFD3FF6062FC21EAF94A" box="[169,215,1684,1708]" pageId="6" pageNumber="4780">type</typeStatus>
strain of
<taxonomicName id="4C07FAC7FF8AFFD3FE8862FB2325F94D" authority="Siebert &amp; Huptas &amp; Wenning &amp; Scherer &amp; Doll, 2020" authorityName="Siebert &amp; Huptas &amp; Wenning &amp; Scherer &amp; Doll" authorityYear="2020" box="[321,536,1683,1707]" class="Bacilli" family="Aerococcaceae" genus="Fundicoccus" higherTaxonomySource="GBIF" kingdom="Bacteria" order="Lactobacillales" pageId="6" pageNumber="4780" phylum="Firmicutes" rank="species" species="ignavus" status="gen. nov., sp. nov.">
<emphasis id="B9735D56FF8AFFD3FE8862FB2325F94D" box="[321,536,1683,1707]" italics="true" pageId="6" pageNumber="4780">Fundicoccus ignavus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A240E02DFF8AFFD3FDE962FC23E2F94D" box="[544,735,1684,1708]" pageId="6" pageNumber="4780" rank="species">gen. nov., sp. nov.</taxonomicNameLabel>
is
<specimenCode id="DBA1293FFF8AFFD3FFB062DA21E4F959" box="[121,217,1713,1738]" pageId="6" pageNumber="4780">
WS4937
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(=DSM 109652
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=LMG 31441
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), which was isolated from raw milk of a farm bulk tank. The DNA G+C content of the type strain is 37.4mol% and the genome size is ~3.0 Mb. WS5303 (=DSM 109653=LMG 31442) and WS4759, two additional strains of the species were isolated from other bulk tank milk samples. Whole-genome sequences of WS4937
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, WS4759 and WS5303 can be accessed with WJQT00000000, WJQS00000000 and WJQR00000000, respectively.
</materialsCitation>
</paragraph>
</subSubSection>
</treatment>
</document>