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<document id="CD5218495F3C087D10FBAA978C51B20E" ID-DOI="10.1093/zoolinnean/zlad001" ID-ISSN="0024-4082" ID-Zenodo-Dep="8141891" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="diego" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="diego" IM.taxonomicNames_approvedBy="diego" IM.treatments_approvedBy="diego" checkinTime="1689234763575" checkinUser="plazi" docAuthor="Surmik, Dawid, Słowiak-Morkovina, Justyna, Szczygielski, Tomasz, Wojtyniak, Marcin, Środek, Dorota, Dulski, Mateusz, Balin, Katarzyna, Krzykawski, Tomasz &amp; Pawlicki, Roman" docDate="2023" docId="0395EB6D3E70FFABEE96E7977CACFE17" docLanguage="en" docName="zlad001.pdf" docOrigin="Zoological Journal of the Linnean Society 198 (3)" docSource="https://academic.oup.com/zoolinnean/article/198/3/747/7076280" docStyle="DocumentStyle:36B3BD6A90C22AB4F7F465C853188CC8.7:ZoolJLinnSoc.2017-.journal_article" docStyleId="36B3BD6A90C22AB4F7F465C853188CC8" docStyleName="ZoolJLinnSoc.2017-.journal_article" docStyleVersion="7" docTitle="Edmontosaurus regalis Lambe 1917" docType="treatment" docVersion="3" lastPageNumber="751" masterDocId="FFAC93153E74FFAFEE07E5747F4CFFF8" masterDocTitle="The first record of fossilized soft parts in ossified tendons and implications for the understanding of tendon mineralization" masterLastPageNumber="766" masterPageNumber="747" pageNumber="751" updateTime="1689337557308" updateUser="diego">
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<mods:title id="F6E13EE1215EACAEDCE0389D04B89E6B">The first record of fossilized soft parts in ossified tendons and implications for the understanding of tendon mineralization</mods:title>
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<taxonomicName id="4C3C21F83E70FFABEE96E7977EE2FD03" authorityName="Lambe" authorityYear="1917" box="[145,430,739,763]" class="Reptilia" family="Hadrosauridae" genus="Edmontosaurus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Ornithischia" pageId="4" pageNumber="751" phylum="Chordata" rank="species" species="regalis">Edmontosaurus regalis</taxonomicName>
(UAMES 52615)
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Both cross-sectioned tendons show a highly porous structure built of fibrous primary matrix (
<figureCitation id="130746FE3E70FFABEC7FE65E7DA7FCB8" box="[632,747,810,832]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1AC</figureCitation>
). The general structure of the sectioned tendons is homogenous. The matrix is composed of coarse collagenous fibre bundles containing numerous bone cell lacunae (
<figureCitation id="130746FE3E70FFABED36E5B17CEAFF22" box="[817,934,197,219]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1A, C</figureCitation>
). The vascular canals forming the porous structure of the tendons are young Haversian canals (
<figureCitation id="130746FE3E70FFABED36E4767CE9FEE0" box="[817,933,258,280]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1AC</figureCitation>
). The cross-sectioned larger tendon shows few scattered secondary osteons in the centre of the tendon surrounded by collagen-fibre bundles (
<figureCitation id="130746FE3E70FFABEB36E4347ACEFEAD" box="[1329,1410,320,342]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1D</figureCitation>
). The tendon of a slightly smaller cross-section (
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diameter;
<figureCitation id="130746FE3E70FFABEDA6E4097B5AFE6B" box="[929,1046,381,403]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1A, B</figureCitation>
) is more porous than the larger one (
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;
<figureCitation id="130746FE3E70FFABEDAEE4E87B5AFE49" box="[937,1046,412,434]" captionStart="Figure 1" captionStartId="4.[146,224,1672,1694]" captionTargetBox="[146,1425,973,1642]" captionTargetId="figure-431@4.[145,1425,972,1643]" captionTargetPageId="4" captionText="Figure 1. Light microscopy and SEM of petrographic thin sections and etched surfaces of fossilized ornithischian tendons of Edmontosaurus regalis: A, B, ground section (transverse) of the smaller tendon under transmitted normal light (A) and polarized light alpha-compensation mode (B) exhibiting primary organization of tissue; C, D, ground section (transverse) of the periphery (C) (under transmitted normal light) and centre (D) (under polarized light) of the larger tendon; E, circular backscatter detector (CBS-SEM) image of the etched surface of the tendon shows structures interpreted as vascular canals with vessel-like morphology and attached cell-like structures, well visible branched protrusions (red arrow) are also visible; F, CBS-SEM image longitudinal section of the vessel-like canals with cell-like structures attached to the wall (blue arrow). White arrows indicate young Haversian canals, green mark secondary osteons, and yellow point at bone cell lacunae." figureDoi="http://doi.org/10.5281/zenodo.8141893" httpUri="https://zenodo.org/record/8141893/files/figure.png" pageId="4" pageNumber="751">Fig. 1C, D</figureCitation>
). The different structure between the sampled tendons may result from a different location in the body.
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