459 lines
118 KiB
XML
459 lines
118 KiB
XML
<document id="A4A7554BFDA23F4B322DF42BFF146837" ID-DOI="10.5281/zenodo.177493" ID-GBIF-Dataset="7a8c9e9e-78bb-476d-80a4-93e455bc3881" ID-ISSN="1175-5326" ID-Zenodo-Dep="177493" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1459863000251" checkinUser="plazi" docAuthor="Peng, Xiaohui, Shi, Xinlu & Warren, Alan" docDate="2007" docId="039E87B667031371FF17FEB8FAB8E1D3" docLanguage="en" docName="zt01524p045.pdf" docOrigin="Zootaxa 1524" docStyle="DocumentStyle:6581673A57F01A3145754A1E615EDFF0.4:Zootaxa.2007-2008.journal_article" docStyleId="6581673A57F01A3145754A1E615EDFF0" docStyleName="Zootaxa.2007-2008.journal_article" docStyleVersion="4" docTitle="Pseudepistylis songi Peng, Shi & Warren, 2007, n. sp." docType="treatment" docVersion="9" lastPageNumber="42" masterDocId="FFA7FFCE67011376FF80FFE7FFFBE459" masterDocTitle="Establishment of a new peritrich ciliate genus, Pseudepistylis n. gen. (Ciliophora: Peritrichia: Epistylididae), with a description of a new freshwater species, Pseudepistylis songi n. sp. from Wenzhou, China" masterLastPageNumber="45" masterPageNumber="35" pageNumber="37" updateTime="1698226216141" updateUser="plazi">
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<mods:titleInfo id="0040D589C335092847BE15458909BFCB">
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<mods:title id="7CC74C30EB1D4A424A90409596924DC4">Establishment of a new peritrich ciliate genus, Pseudepistylis n. gen. (Ciliophora: Peritrichia: Epistylididae), with a description of a new freshwater species, Pseudepistylis songi n. sp. from Wenzhou, China</mods:title>
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<mods:name id="CC465E7734BDCCF153A3A6E9E352462F" type="personal">
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<mods:roleTerm id="6B59274AC132E13734868059EAB1AAD4">Author</mods:roleTerm>
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<mods:namePart id="FF1BFC58056BDF05ECCDFE8DDB3A531B">Peng, Xiaohui</mods:namePart>
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<mods:roleTerm id="5A70995B8718A4D85BDE8D382DC9CCF8">Author</mods:roleTerm>
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<mods:namePart id="89447CA5CC3556E22854FE6170931EE2">Shi, Xinlu</mods:namePart>
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<mods:roleTerm id="2331B25685BD341536B2BEE30587609D">Author</mods:roleTerm>
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<mods:namePart id="8AFC24EEC3CE2BCF339E6CFB1B41F2F8">Warren, Alan</mods:namePart>
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<mods:typeOfResource id="B6DC309417F9023B724DCE228968069E">text</mods:typeOfResource>
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<mods:title id="C42D603495CED2F2C28BD500B7CD6DBD">Zootaxa</mods:title>
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<mods:part id="1BD54340BB3F018C397372AC8E591E45">
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<mods:date id="C8C0C78033F985BE6FC57A32CE30CB94">2007</mods:date>
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<mods:detail id="6975BA8147B71B360E47596072638DAB" type="volume">
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<mods:number id="6507B3B43FBE5703CE22450869EC288E">1524</mods:number>
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<mods:classification id="431CB6F6DEBECBABC14576CB38CDBF4B">journal article</mods:classification>
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<mods:identifier id="C022C982731C1FED4F8A4CBB1282802A" type="DOI">10.5281/zenodo.177493</mods:identifier>
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<mods:identifier id="1295376334FF4E45B7836CEA40BEF642" type="ISSN">1175-5326</mods:identifier>
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<treatment id="039E87B667031371FF17FEB8FAB8E1D3" ID-DOI="http://doi.org/10.5281/zenodo.5625916" ID-GBIF-Taxon="119368760" ID-Zenodo-Dep="5625916" LSID="urn:lsid:plazi:treatment:039E87B667031371FF17FEB8FAB8E1D3" httpUri="http://treatment.plazi.org/id/039E87B667031371FF17FEB8FAB8E1D3" lastPageId="7" lastPageNumber="42" pageId="2" pageNumber="37">
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<subSubSection id="C32D652B67031374FF17FEB8FE77E5C5" pageId="2" pageNumber="37" type="nomenclature">
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<paragraph id="8B8836A067031374FF17FEB8FE29E520" blockId="2.[151,466,351,412]" box="[151,466,351,377]" pageId="2" pageNumber="37">
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<heading id="D0C081CC67031374FF17FEB8FE29E520" bold="true" box="[151,466,351,377]" fontSize="11" level="1" pageId="2" pageNumber="37" reason="1">
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<emphasis id="B943EAB267031374FF17FEB8FE29E520" bold="true" box="[151,466,351,377]" pageId="2" pageNumber="37">
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<taxonomicName id="4C374D2367031374FF17FEB8FE73E520" box="[151,392,351,377]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="2" pageNumber="37" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
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<emphasis id="B943EAB267031374FF17FEB8FE73E520" bold="true" box="[151,392,351,377]" italics="true" pageId="2" pageNumber="37">Pseudepistylis songi</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="A27057C967031374FE10FEB8FE29E520" box="[400,466,351,377]" pageId="2" pageNumber="37" rank="species">n. sp.</taxonomicNameLabel>
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</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8B8836A067031374FF17FE65FE77E5C5" blockId="2.[151,466,351,412]" box="[151,396,386,412]" pageId="2" pageNumber="37">
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(
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<tableCitation id="C6B5031B67031374FF1EFE65FF03E5C5" box="[158,248,386,412]" captionStart="TABLE 1" captionStartId="2.[151,239,1509,1533]" captionTargetBox="[159,1427,1565,1935]" captionTargetPageId="2" captionText="TABLE 1. Morphological characterization of Pseudepistylis songi n. sp. (all measurements in μm)." httpUri="http://table.plazi.org/id/DF48662867031374FF17FA02FB46E1A4" pageId="2" pageNumber="37" tableUuid="DF48662867031374FF17FA02FB46E1A4">Table 1</tableCitation>
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;
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<figureCitation id="130C2A2567031374FE87FE65FE78E5C5" box="[263,387,386,412]" captionStart-0="FIGURES 1 – 7" captionStart-1="FIGURES 8 – 10" captionStart-2="FIGURES 11 – 21" captionStart-3="FIGURES 22 – 37" captionStartId-0="3.[151,269,1373,1397]" captionStartId-1="4.[151,269,1075,1099]" captionStartId-2="5.[151,269,1867,1891]" captionStartId-3="6.[151,269,1771,1795]" captionTargetBox-0="[156,1427,203,1345]" captionTargetBox-1="[204,1404,200,1067]" captionTargetBox-2="[248,1346,180,1858]" captionTargetBox-3="[199,1383,196,1749]" captionTargetId-0="figure@3.[151,1436,197,1346]" captionTargetId-1="figure@4.[195,1409,194,1071]" captionTargetId-2="figure@5.[246,1348,180,1861]" captionTargetId-3="figure@6.[197,1384,194,1750]" captionTargetPageId-0="3" captionTargetPageId-1="4" captionTargetPageId-2="5" captionTargetPageId-3="6" captionText-0="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." captionText-1="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." captionText-2="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." captionText-3="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri-0="https://zenodo.org/record/177494/files/figure.png" httpUri-1="https://zenodo.org/record/177495/files/figure.png" httpUri-2="https://zenodo.org/record/177496/files/figure.png" httpUri-3="https://zenodo.org/record/177497/files/figure.png" pageId="2" pageNumber="37">Figs. 1–37</figureCitation>
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)
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</paragraph>
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</subSubSection>
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<subSubSection id="C32D652B67031374FF17FE35FE6AE685" pageId="2" pageNumber="37" type="diagnosis">
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<paragraph id="8B8836A067031374FF17FE35FE6AE685" blockId="2.[151,1436,466,1455]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF17FE35FEE2E5B5" bold="true" box="[151,281,466,492]" pageId="2" pageNumber="37">Diagnosis:</emphasis>
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Limnetic species with transparent or translucent, dichotomously branched stalk. Zooids elongate, bell-shaped, measuring 220 × 140 μm in vivo on average, with thick peristomial lip that is invariably reflected aborally. One contractile vacuole located on dorsal wall of infundibulum. Macronucleus C-shaped and transversely oriented. More than 137 transverse silverlines between oral area and aboral ciliary wreath, on average 77 between aboral ciliary wreath and scopula. Haplokinety and polykinety make one and one-quarter turns on peristome; terminal ends of kineties of infundibular polykinety 2 terminate adstomally at different levels within infundibulum.
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</paragraph>
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</subSubSection>
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<subSubSection id="C32D652B67031374FF46FD0DFC30E036" pageId="2" pageNumber="37" type="materials_examined">
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<paragraph id="8B8836A067031374FF46FD0DFED7E7FD" blockId="2.[151,1436,466,1455]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF46FD0DFE5AE75D" bold="true" box="[198,417,746,772]" pageId="2" pageNumber="37">
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<typeStatus id="548C880267031374FF46FD0DFEF8E75D" box="[198,259,746,772]" pageId="2" pageNumber="37">Type</typeStatus>
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specimens:
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</emphasis>
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One
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<typeStatus id="548C880267031374FE7DFD0DFD9FE75D" box="[509,612,746,772]" pageId="2" pageNumber="37" type="holotype">holotype</typeStatus>
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(registration number WZ2005102504) and one
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<typeStatus id="548C880267031374FB6BFD0DFAABE75D" box="[1259,1360,746,772]" pageId="2" pageNumber="37" type="paratype">paratype</typeStatus>
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slide (WZ2006110204) with protargol and silver nitrate impregnated specimens respectively are deposited in the Laboratory of Zoological Systematics, Hangzhou Normal University,
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<collectingCountry id="F320763067031374FC4EFCDDFBE3E70D" box="[974,1048,826,852]" name="China" pageId="2" pageNumber="37">China</collectingCountry>
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. A second
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<typeStatus id="548C880267031374FB1AFCDDFB04E70D" box="[1178,1279,826,852]" pageId="2" pageNumber="37" type="paratype">paratype</typeStatus>
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of protargolstained specimens is deposited in the Natural History Museum, London,
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<collectingCountry id="F320763067031374FBB3FC85FBA6E725" box="[1075,1117,866,892]" name="United Kingdom" pageId="2" pageNumber="37">UK</collectingCountry>
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with registration number 2006:6:20:1.
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</paragraph>
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<paragraph id="8B8836A067031374FF46FC55FDCEE045" blockId="2.[151,1436,466,1455]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF46FC55FEACE795" bold="true" box="[198,343,946,972]" pageId="2" pageNumber="37">Dedication:</emphasis>
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We dedicate this new species to Prof. Dr. Weibo Song, Director of Laboratory of Protozoology, Ocean University of
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<collectingCountry id="F320763067031374FE45FC3DFDF5E7AD" box="[453,526,986,1012]" name="China" pageId="2" pageNumber="37">China</collectingCountry>
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, Qingdao,
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<collectingCountry id="F320763067031374FD0EFC3DFD2CE7AD" box="[654,727,986,1012]" name="China" pageId="2" pageNumber="37">China</collectingCountry>
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, in acknowledgement of his outstanding contributions to the study of ciliated protozoa in
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<collectingCountry id="F320763067031374FE67FBE5FDCAE045" box="[487,561,1026,1052]" name="China" pageId="2" pageNumber="37">China</collectingCountry>
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.
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</paragraph>
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<paragraph id="8B8836A067031374FF46FBCDFAD5E01D" blockId="2.[151,1436,466,1455]" box="[198,1326,1066,1092]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF46FBCDFE94E01D" bold="true" box="[198,367,1066,1092]" pageId="2" pageNumber="37">
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<typeStatus id="548C880267031374FF46FBCDFEFFE01D" box="[198,260,1066,1092]" pageId="2" pageNumber="37">Type</typeStatus>
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locality:
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</emphasis>
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Freshwater pond, Lishui Street, Wenzhou,
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<collectingCountry id="F320763067031374FCEBFBCDFC4AE01D" box="[875,945,1066,1092]" name="China" pageId="2" pageNumber="37">China</collectingCountry>
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(
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<geoCoordinate id="EE03506767031374FC41FBCDFB9DE01D" box="[961,1126,1066,1092]" direction="north" orientation="latitude" pageId="2" pageNumber="37" precision="1" value="28.3403">N 28°20.418’</geoCoordinate>
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;
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<geoCoordinate id="EE03506767031374FBF1FBCDFAD9E01D" box="[1137,1314,1066,1092]" direction="east" orientation="longitude" pageId="2" pageNumber="37" precision="1" value="120.730286">E 120°43.817’</geoCoordinate>
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).
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</paragraph>
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<paragraph id="8B8836A067031374FF46FBB2FC30E036" blockId="2.[151,1436,466,1455]" box="[198,971,1106,1135]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF46FBB2FE7EE036" bold="true" box="[198,389,1109,1135]" pageId="2" pageNumber="37">Ecological data</emphasis>
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: Freshwater, water temperature 22.4 o C,
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<collectingCountry id="F320763067031374FCE5FBB2FC72E036" box="[869,905,1109,1135]" name="Philippines" pageId="2" pageNumber="37">pH</collectingCountry>
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7.34.
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</paragraph>
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</subSubSection>
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<subSubSection id="C32D652B67031371FF46FB9AFAB8E1D3" lastPageId="7" lastPageNumber="42" pageId="2" pageNumber="37" type="description">
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<paragraph id="8B8836A067031374FF46FB9AFECEE1F6" blockId="2.[151,1436,466,1455]" pageId="2" pageNumber="37">
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<emphasis id="B943EAB267031374FF46FB9AFE9AE0CE" bold="true" box="[198,353,1149,1175]" pageId="2" pageNumber="37">Description:</emphasis>
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The zooid is bell-shaped,
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<emphasis id="B943EAB267031374FD1CFB99FD10E0CE" box="[668,747,1150,1175]" italics="true" pageId="2" pageNumber="37">in vivo</emphasis>
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170–285 μm long and 110–190 μm wide; length to width ratio of the fully extended zooid was 1.30–1.85. The peristomial lip is much wider than greatest body width, thick, and invariably reflected aborally. The peristomial disc is large, dome-shaped, and conspicuously elevated above the peristomial lip (
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<figureCitation id="130C2A2567031374FD91FB12FD98E156" box="[529,611,1269,1295]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="2" pageNumber="37">Figs. 1</figureCitation>
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,
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<figureCitation id="130C2A2567031374FDF1FB12FD6BE156" box="[625,656,1269,1295]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="2" pageNumber="37">11</figureCitation>
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). Contracted zooids are ovoid or dumbbell-shaped (
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<figureCitation id="130C2A2567031374FB72FB12FABFE156" box="[1266,1348,1269,1295]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="2" pageNumber="37">Figs. 6</figureCitation>
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a,b, 15) with several conspicuous folds, usually numbering 3–4 adoral to the aboral ciliary wreath and 2–3 between the aboral ciliary wreath and the scopula (
|
||
<figureCitation id="130C2A2567031374FDFFFAA2FD2AE106" box="[639,721,1349,1375]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="2" pageNumber="37">Figs. 6</figureCitation>
|
||
a,b, 12, arrowhead). The pellicle appears smooth at low magnification but fine, reticulate striations are visible at higher magnifications (
|
||
<figureCitation id="130C2A2567031374FBAFFA8AFB7DE1DE" box="[1071,1158,1389,1415]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="2" pageNumber="37">Figs. 5</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567031374FB16FA8AFB4CE1DE" box="[1174,1207,1389,1415]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="2" pageNumber="37">30</figureCitation>
|
||
). Colonies without macrozooids.
|
||
</paragraph>
|
||
<caption id="DF48662867031374FF17FA02FB46E1A4" ID-Table-UUID="DF48662867031374FF17FA02FB46E1A4" box="[151,1213,1509,1533]" httpUri="http://table.plazi.org/id/DF48662867031374FF17FA02FB46E1A4" pageId="2" pageNumber="37" targetBox="[159,1427,1565,1935]" targetIsTable="true" targetPageId="2">
|
||
<paragraph id="8B8836A067031374FF17FA02FB46E1A4" blockId="2.[151,1213,1509,1533]" box="[151,1213,1509,1533]" pageId="2" pageNumber="37">
|
||
<emphasis id="B943EAB267031374FF17FA02FEF3E1A4" bold="true" box="[151,264,1509,1533]" pageId="2" pageNumber="37">TABLE 1.</emphasis>
|
||
Morphological characterization of
|
||
<taxonomicName id="4C374D2367031374FD03FA02FCA7E1A5" box="[643,860,1509,1532]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="2" pageNumber="37" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
|
||
<emphasis id="B943EAB267031374FD03FA02FCA7E1A5" box="[643,860,1509,1532]" italics="true" pageId="2" pageNumber="37">Pseudepistylis songi</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A27057C967031374FCE3FA02FC64E1A4" box="[867,927,1509,1533]" pageId="2" pageNumber="37" rank="species">
|
||
<emphasis id="B943EAB267031374FCE3FA02FC64E1A4" bold="true" box="[867,927,1509,1533]" pageId="2" pageNumber="37">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
(all measurements in μm).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B8836A067031374FF1FF9FAFA99E3D5" pageId="2" pageNumber="37">
|
||
<table id="F937C4006703EC89FF1FF9FAFA68E3D6" box="[159,1427,1565,1935]" gridcols="8" gridrows="9" pageId="2" pageNumber="37">
|
||
<tr id="350734E26703EC89FF1FF9FAFA68E26D" box="[159,1427,1565,1588]" gridrow="0" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF9FAFD51E26D" box="[159,682,1565,1588]" gridcol="0" gridrow="0" pageId="2" pageNumber="37">Character</th>
|
||
<th id="76D65D9E6703EC89FD6EF9FAFCF2E26D" box="[750,777,1565,1588]" gridcol="1" gridrow="0" pageId="2" pageNumber="37">n</th>
|
||
<th id="76D65D9E6703EC89FCBCF9FAFC93E26D" box="[828,872,1565,1588]" gridcol="2" gridrow="0" pageId="2" pageNumber="37">Min</th>
|
||
<th id="76D65D9E6703EC89FC23F9FAFC2FE26D" box="[931,980,1565,1588]" gridcol="3" gridrow="0" pageId="2" pageNumber="37">Max</th>
|
||
<th id="76D65D9E6703EC89FB8AF9FAFBBCE26D" box="[1034,1095,1565,1588]" gridcol="4" gridrow="0" pageId="2" pageNumber="37">Mean</th>
|
||
<th id="76D65D9E6703EC89FBF2F9FAFB55E26D" box="[1138,1198,1565,1588]" gridcol="5" gridrow="0" pageId="2" pageNumber="37">SD</th>
|
||
<th id="76D65D9E6703EC89FB59F9FAFAF3E26D" box="[1241,1288,1565,1588]" gridcol="6" gridrow="0" pageId="2" pageNumber="37">SE</th>
|
||
<th id="76D65D9E6703EC89FAC0F9FAFA68E26D" box="[1344,1427,1565,1588]" gridcol="7" gridrow="0" pageId="2" pageNumber="37">CV (%)</th>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF9AFFA68E206" box="[159,1427,1608,1631]" gridrow="1" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF9AFFD51E206" box="[159,682,1608,1631]" gridcol="0" gridrow="1" pageId="2" pageNumber="37">Length of zooid, in vivo</th>
|
||
<td id="76D65D9E6703EC89FD6EF9AFFCF2E206" box="[750,777,1608,1631]" gridcol="1" gridrow="1" pageId="2" pageNumber="37">18</td>
|
||
<td id="76D65D9E6703EC89FCBCF9AFFC93E206" box="[828,872,1608,1631]" gridcol="2" gridrow="1" pageId="2" pageNumber="37">170</td>
|
||
<td id="76D65D9E6703EC89FC23F9AFFC2FE206" box="[931,980,1608,1631]" gridcol="3" gridrow="1" pageId="2" pageNumber="37">285</td>
|
||
<td id="76D65D9E6703EC89FB8AF9AFFBBCE206" box="[1034,1095,1608,1631]" gridcol="4" gridrow="1" pageId="2" pageNumber="37">220</td>
|
||
<td id="76D65D9E6703EC89FBF2F9AFFB55E206" box="[1138,1198,1608,1631]" gridcol="5" gridrow="1" pageId="2" pageNumber="37">31.90</td>
|
||
<td id="76D65D9E6703EC89FB59F9AFFAF3E206" box="[1241,1288,1608,1631]" gridcol="6" gridrow="1" pageId="2" pageNumber="37">7.74</td>
|
||
<td id="76D65D9E6703EC89FAC0F9AFFA68E206" box="[1344,1427,1608,1631]" gridcol="7" gridrow="1" pageId="2" pageNumber="37">14.5</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF994FA68E2D3" box="[159,1427,1651,1674]" gridrow="2" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF994FD51E2D3" box="[159,682,1651,1674]" gridcol="0" gridrow="2" pageId="2" pageNumber="37">Width of zooid, in vivo</th>
|
||
<td id="76D65D9E6703EC89FD6EF994FCF2E2D3" box="[750,777,1651,1674]" gridcol="1" gridrow="2" pageId="2" pageNumber="37">18</td>
|
||
<td id="76D65D9E6703EC89FCBCF994FC93E2D3" box="[828,872,1651,1674]" gridcol="2" gridrow="2" pageId="2" pageNumber="37">110</td>
|
||
<td id="76D65D9E6703EC89FC23F994FC2FE2D3" box="[931,980,1651,1674]" gridcol="3" gridrow="2" pageId="2" pageNumber="37">190</td>
|
||
<td id="76D65D9E6703EC89FB8AF994FBBCE2D3" box="[1034,1095,1651,1674]" gridcol="4" gridrow="2" pageId="2" pageNumber="37">140</td>
|
||
<td id="76D65D9E6703EC89FBF2F994FB55E2D3" box="[1138,1198,1651,1674]" gridcol="5" gridrow="2" pageId="2" pageNumber="37">22.91</td>
|
||
<td id="76D65D9E6703EC89FB59F994FAF3E2D3" box="[1241,1288,1651,1674]" gridcol="6" gridrow="2" pageId="2" pageNumber="37">5.56</td>
|
||
<td id="76D65D9E6703EC89FAC0F994FA68E2D3" box="[1344,1427,1651,1674]" gridcol="7" gridrow="2" pageId="2" pageNumber="37">16.3</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF97AFA68E2ED" box="[159,1427,1693,1716]" gridrow="3" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF97AFD51E2ED" box="[159,682,1693,1716]" gridcol="0" gridrow="3" pageId="2" pageNumber="37">Width of peristomial disc, in vivo</th>
|
||
<td id="76D65D9E6703EC89FD6EF97AFCF2E2ED" box="[750,777,1693,1716]" gridcol="1" gridrow="3" pageId="2" pageNumber="37">18</td>
|
||
<td id="76D65D9E6703EC89FCBCF97AFC93E2ED" box="[828,872,1693,1716]" gridcol="2" gridrow="3" pageId="2" pageNumber="37">150</td>
|
||
<td id="76D65D9E6703EC89FC23F97AFC2FE2ED" box="[931,980,1693,1716]" gridcol="3" gridrow="3" pageId="2" pageNumber="37">230</td>
|
||
<td id="76D65D9E6703EC89FB8AF97AFBBCE2ED" box="[1034,1095,1693,1716]" gridcol="4" gridrow="3" pageId="2" pageNumber="37">180</td>
|
||
<td id="76D65D9E6703EC89FBF2F97AFB55E2ED" box="[1138,1198,1693,1716]" gridcol="5" gridrow="3" pageId="2" pageNumber="37">22.51</td>
|
||
<td id="76D65D9E6703EC89FB59F97AFAF3E2ED" box="[1241,1288,1693,1716]" gridcol="6" gridrow="3" pageId="2" pageNumber="37">5.46</td>
|
||
<td id="76D65D9E6703EC89FAC0F97AFA68E2ED" box="[1344,1427,1693,1716]" gridcol="7" gridrow="3" pageId="2" pageNumber="37">12.4</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF92FFA68E286" box="[159,1427,1736,1759]" gridrow="4" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF92FFD51E286" box="[159,682,1736,1759]" gridcol="0" gridrow="4" pageId="2" pageNumber="37">Length of zooid, protargol-impregnated</th>
|
||
<td id="76D65D9E6703EC89FD6EF92FFCF2E286" box="[750,777,1736,1759]" gridcol="1" gridrow="4" pageId="2" pageNumber="37">24</td>
|
||
<td id="76D65D9E6703EC89FCBCF92FFC93E286" box="[828,872,1736,1759]" gridcol="2" gridrow="4" pageId="2" pageNumber="37">106</td>
|
||
<td id="76D65D9E6703EC89FC23F92FFC2FE286" box="[931,980,1736,1759]" gridcol="3" gridrow="4" pageId="2" pageNumber="37">197</td>
|
||
<td id="76D65D9E6703EC89FB8AF92FFBBCE286" box="[1034,1095,1736,1759]" gridcol="4" gridrow="4" pageId="2" pageNumber="37">151.3</td>
|
||
<td id="76D65D9E6703EC89FBF2F92FFB55E286" box="[1138,1198,1736,1759]" gridcol="5" gridrow="4" pageId="2" pageNumber="37">29.06</td>
|
||
<td id="76D65D9E6703EC89FB59F92FFAF3E286" box="[1241,1288,1736,1759]" gridcol="6" gridrow="4" pageId="2" pageNumber="37">6.06</td>
|
||
<td id="76D65D9E6703EC89FAC0F92FFA68E286" box="[1344,1427,1736,1759]" gridcol="7" gridrow="4" pageId="2" pageNumber="37">19.2</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF914FA68E353" box="[159,1427,1779,1802]" gridrow="5" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF914FD51E353" box="[159,682,1779,1802]" gridcol="0" gridrow="5" pageId="2" pageNumber="37">Width of zooid, protargol-impregnated</th>
|
||
<td id="76D65D9E6703EC89FD6EF914FCF2E353" box="[750,777,1779,1802]" gridcol="1" gridrow="5" pageId="2" pageNumber="37">24</td>
|
||
<td id="76D65D9E6703EC89FCBCF914FC93E353" box="[828,872,1779,1802]" gridcol="2" gridrow="5" pageId="2" pageNumber="37">86</td>
|
||
<td id="76D65D9E6703EC89FC23F914FC2FE353" box="[931,980,1779,1802]" gridcol="3" gridrow="5" pageId="2" pageNumber="37">207</td>
|
||
<td id="76D65D9E6703EC89FB8AF914FBBCE353" box="[1034,1095,1779,1802]" gridcol="4" gridrow="5" pageId="2" pageNumber="37">131.9</td>
|
||
<td id="76D65D9E6703EC89FBF2F914FB55E353" box="[1138,1198,1779,1802]" gridcol="5" gridrow="5" pageId="2" pageNumber="37">31.29</td>
|
||
<td id="76D65D9E6703EC89FB59F914FAF3E353" box="[1241,1288,1779,1802]" gridcol="6" gridrow="5" pageId="2" pageNumber="37">6.52</td>
|
||
<td id="76D65D9E6703EC89FAC0F914FA68E353" box="[1344,1427,1779,1802]" gridcol="7" gridrow="5" pageId="2" pageNumber="37">23.7</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF8FAFA68E36D" box="[159,1427,1821,1844]" gridrow="6" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF8FAFD51E36D" box="[159,682,1821,1844]" gridcol="0" gridrow="6" pageId="2" pageNumber="37">Width of peristomial disc, protargol-impregnated</th>
|
||
<td id="76D65D9E6703EC89FD6EF8FAFCF2E36D" box="[750,777,1821,1844]" gridcol="1" gridrow="6" pageId="2" pageNumber="37">24</td>
|
||
<td id="76D65D9E6703EC89FCBCF8FAFC93E36D" box="[828,872,1821,1844]" gridcol="2" gridrow="6" pageId="2" pageNumber="37">56</td>
|
||
<td id="76D65D9E6703EC89FC23F8FAFC2FE36D" box="[931,980,1821,1844]" gridcol="3" gridrow="6" pageId="2" pageNumber="37">131</td>
|
||
<td id="76D65D9E6703EC89FB8AF8FAFBBCE36D" box="[1034,1095,1821,1844]" gridcol="4" gridrow="6" pageId="2" pageNumber="37">91.4</td>
|
||
<td id="76D65D9E6703EC89FBF2F8FAFB55E36D" box="[1138,1198,1821,1844]" gridcol="5" gridrow="6" pageId="2" pageNumber="37">17.13</td>
|
||
<td id="76D65D9E6703EC89FB59F8FAFAF3E36D" box="[1241,1288,1821,1844]" gridcol="6" gridrow="6" pageId="2" pageNumber="37">3.57</td>
|
||
<td id="76D65D9E6703EC89FAC0F8FAFA68E36D" box="[1344,1427,1821,1844]" gridcol="7" gridrow="6" pageId="2" pageNumber="37">18.7</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF8AFFA68E33B" box="[159,1427,1864,1890]" gridrow="7" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF8AFFD51E33B" box="[159,682,1864,1890]" gridcol="0" gridrow="7" pageId="2" pageNumber="37">Number of silverlines from peristome to ACW</th>
|
||
<td id="76D65D9E6703EC89FD6EF8AFFCF2E33B" box="[750,777,1864,1890]" gridcol="1" gridrow="7" pageId="2" pageNumber="37">13</td>
|
||
<td id="76D65D9E6703EC89FCBCF8AFFC93E33B" box="[828,872,1864,1890]" gridcol="2" gridrow="7" pageId="2" pageNumber="37">125</td>
|
||
<td id="76D65D9E6703EC89FC23F8AFFC2FE33B" box="[931,980,1864,1890]" gridcol="3" gridrow="7" pageId="2" pageNumber="37">145</td>
|
||
<td id="76D65D9E6703EC89FB8AF8AFFBBCE33B" box="[1034,1095,1864,1890]" gridcol="4" gridrow="7" pageId="2" pageNumber="37">136.6</td>
|
||
<td id="76D65D9E6703EC89FBF2F8AFFB55E33B" box="[1138,1198,1864,1890]" gridcol="5" gridrow="7" pageId="2" pageNumber="37">7.49</td>
|
||
<td id="76D65D9E6703EC89FB59F8AFFAF3E33B" box="[1241,1288,1864,1890]" gridcol="6" gridrow="7" pageId="2" pageNumber="37">2.16</td>
|
||
<td id="76D65D9E6703EC89FAC0F8AFFA68E33B" box="[1344,1427,1864,1890]" gridcol="7" gridrow="7" pageId="2" pageNumber="37">5.5</td>
|
||
</tr>
|
||
<tr id="350734E26703EC89FF1FF892FA68E3D6" box="[159,1427,1909,1935]" gridrow="8" pageId="2" pageNumber="37">
|
||
<th id="76D65D9E6703EC89FF1FF892FD51E3D6" box="[159,682,1909,1935]" gridcol="0" gridrow="8" pageId="2" pageNumber="37">Number of silverlines from ACW to scopula</th>
|
||
<td id="76D65D9E6703EC89FD6EF892FCF2E3D6" box="[750,777,1909,1935]" gridcol="1" gridrow="8" pageId="2" pageNumber="37">13</td>
|
||
<td id="76D65D9E6703EC89FCBCF892FC93E3D6" box="[828,872,1909,1935]" gridcol="2" gridrow="8" pageId="2" pageNumber="37">70</td>
|
||
<td id="76D65D9E6703EC89FC23F892FC2FE3D6" box="[931,980,1909,1935]" gridcol="3" gridrow="8" pageId="2" pageNumber="37">80</td>
|
||
<td id="76D65D9E6703EC89FB8AF892FBBCE3D6" box="[1034,1095,1909,1935]" gridcol="4" gridrow="8" pageId="2" pageNumber="37">76.6</td>
|
||
<td id="76D65D9E6703EC89FBF2F892FB55E3D6" box="[1138,1198,1909,1935]" gridcol="5" gridrow="8" pageId="2" pageNumber="37">2.66</td>
|
||
<td id="76D65D9E6703EC89FB59F892FAF3E3D6" box="[1241,1288,1909,1935]" gridcol="6" gridrow="8" pageId="2" pageNumber="37">0.77</td>
|
||
<td id="76D65D9E6703EC89FAC0F892FA68E3D6" box="[1344,1427,1909,1935]" gridcol="7" gridrow="8" pageId="2" pageNumber="37">3.5</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<caption id="DF48662867021375FF17FABAFB17E21D" httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38" targetBox="[156,1427,203,1345]" targetPageId="3">
|
||
<paragraph id="8B8836A067021375FF17FABAFB17E21D" blockId="3.[151,1437,1373,1605]" pageId="3" pageNumber="38">
|
||
<emphasis id="B943EAB267021375FF17FABAFEB8E12C" bold="true" box="[151,323,1373,1397]" pageId="3" pageNumber="38">FIGURES 1–7.</emphasis>
|
||
<taxonomicName id="4C374D2367021375FECBFABAFDDEE12D" box="[331,549,1373,1396]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="3" pageNumber="38" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
|
||
<emphasis id="B943EAB267021375FECBFABAFDDEE12D" box="[331,549,1373,1396]" italics="true" pageId="3" pageNumber="38">Pseudepistylis songi</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A27057C967021375FDACFABAFD92E12C" box="[556,617,1373,1397]" pageId="3" pageNumber="38" rank="species">
|
||
<emphasis id="B943EAB267021375FDACFABAFD92E12C" bold="true" box="[556,617,1373,1397]" pageId="3" pageNumber="38">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
in vivo (1–4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100μm (Fig. 1), 600 μm (Fig. 3).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B8836A067021375FF46F99FFD33E36B" blockId="3.[151,1436,1654,2002]" pageId="3" pageNumber="38">
|
||
The cytoplasm is grey or colourless, containing many large food vacuoles 13–40 μm in diameter (
|
||
<figureCitation id="130C2A2567021375FAC4F99FFF5DE2E3" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Figs. 1, 6</figureCitation>
|
||
b, 11–15). There is one contractile vacuole, ca. 29–37 μm in diameter, located on the dorsal wall of the infundibulum slightly aboral to the peristomial disc (
|
||
<figureCitation id="130C2A2567021375FC86F92FFCA3E2BB" box="[774,856,1736,1762]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Figs. 1</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567021375FCE7F92FFC7DE2BB" box="[871,902,1736,1762]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="3" pageNumber="38">11</figureCitation>
|
||
). The thick, C-shaped macronucleus is transversely oriented and located in the mid-body region (
|
||
<figureCitation id="130C2A2567021375FC9FF917FC8FE353" box="[799,884,1776,1802]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Figs. 1</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567021375FC06F917FC5EE353" box="[902,933,1776,1802]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="3" pageNumber="38">18</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567021375FC35F917FC2DE353" box="[949,982,1776,1802]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="3" pageNumber="38">33</figureCitation>
|
||
); the micronucleus lies near the midregion of the macronucleus (
|
||
<figureCitation id="130C2A2567021375FE66F8FFFDCCE36B" box="[486,567,1816,1842]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Figs. 1</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567021375FDC6F8FFFD9DE36B" box="[582,614,1816,1842]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="3" pageNumber="38">33</figureCitation>
|
||
, arrow).
|
||
</paragraph>
|
||
<paragraph id="8B8836A067021375FF46F8A7FC21E38B" blockId="3.[151,1436,1654,2002]" pageId="3" pageNumber="38">
|
||
The stalk is transparent or translucent, dichotomously branched, and slender, with a main trunk measuring approximately 500 μm long and 9 μm in diameter and zooid-bearing branches measuring 400–500 μm long and 10 μm in diameter (
|
||
<figureCitation id="130C2A2567021375FE3AF877FDF5E3F3" box="[442,526,1936,1962]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Figs. 3</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567021375FD9EF877FD96E3F3" box="[542,621,1936,1962]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="3" pageNumber="38">20, 21</figureCitation>
|
||
). The stalk surface is smooth. In culture, colonies usually contained only one or two zooids, occasionally four, rarely eight or more (
|
||
<figureCitation id="130C2A2567021375FC03F85FFC31E38B" box="[899,970,1976,2002]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="3" pageNumber="38">Fig. 3</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF48662867051372FF17FBD4FBCAE0A1" httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39" targetBox="[204,1404,200,1067]" targetPageId="4">
|
||
<paragraph id="8B8836A067051372FF17FBD4FBCAE0A1" blockId="4.[151,1436,1075,1272]" pageId="4" pageNumber="39">
|
||
<emphasis id="B943EAB267051372FF17FBD4FEABE012" bold="true" box="[151,336,1075,1099]" pageId="4" pageNumber="39">FIGURES 8–10.</emphasis>
|
||
Infraciliature of oral apparatus of
|
||
<taxonomicName id="4C374D2367051372FD3DFBD3FC6DE012" box="[701,918,1076,1099]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="4" pageNumber="39" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
|
||
<emphasis id="B943EAB267051372FD3DFBD3FC6DE012" box="[701,918,1076,1099]" italics="true" pageId="4" pageNumber="39">Pseudepistylis songi</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A27057C967051372FC1CFBD4FC23E012" box="[924,984,1075,1099]" pageId="4" pageNumber="39" rank="species">
|
||
<emphasis id="B943EAB267051372FC1CFBD4FC23E012" bold="true" box="[924,984,1075,1099]" pageId="4" pageNumber="39">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P3 (arrowhead) and the inner row of P3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P3 (arrowhead), the shorter inner row of P3 (double-arrowhead) and the short additional branch of P2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P1–P3, infundibular polykineties 1–3; Po, polykinety (peristomial continuation of P1).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B8836A067051372FF46FACBFEE9E1BF" blockId="4.[151,1436,1324,2033]" pageId="4" pageNumber="39">
|
||
Zooids are very sensitive to stimuli and readily detach from the stalk to become free-swimming by means of their prominent oral cilia (
|
||
<figureCitation id="130C2A2567051372FE6FFAB3FDBFE137" box="[495,580,1364,1390]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="4" pageNumber="39">Figs. 4</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567051372FDD1FAB3FD30E137" box="[593,715,1364,1390]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="4" pageNumber="39">14, 16, 17</figureCitation>
|
||
); free-swimming zooids usually have several pellicular folds (
|
||
<figureCitation id="130C2A2567051372FF1FFA9BFF0FE1CF" box="[159,244,1404,1430]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="4" pageNumber="39">Figs. 4</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567051372FE81FA9BFEDBE1CF" box="[257,288,1404,1430]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="4" pageNumber="39">14</figureCitation>
|
||
, arrowhead). The telotroch is cylindrical, measuring approximately 220 × 120 μm
|
||
<emphasis id="B943EAB267051372FB74FA9BFAB8E1CC" box="[1268,1347,1404,1429]" italics="true" pageId="4" pageNumber="39">in vivo</emphasis>
|
||
, with a conical posterior and domed-shaped anterior protuberance (
|
||
<figureCitation id="130C2A2567051372FCDBFA43FC4BE1E7" box="[859,944,1444,1470]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="4" pageNumber="39">Figs. 2</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567051372FC3EFA43FC25E1E7" box="[958,990,1444,1470]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="4" pageNumber="39">19</figureCitation>
|
||
). Detached zooids can transform into telotrochs.
|
||
</paragraph>
|
||
<paragraph id="8B8836A067051372FF46FA13FBD3E3A8" blockId="4.[151,1436,1324,2033]" pageId="4" pageNumber="39">
|
||
The haplokinety and polykinety of the peristomial infraciliature make approximately one and one-quarter circuits (450o) around the peristome before plunging into the infundibulum, where they make a further complete circuit (
|
||
<figureCitation id="130C2A2567051372FEB9F9A0FE6EE238" box="[313,405,1607,1633]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Fig. 10</figureCitation>
|
||
). The haplokinety and polykinety at the peristomial region are folded after protargol impregnation (
|
||
<figureCitation id="130C2A2567051372FEC4F988FE5CE2D0" box="[324,423,1647,1673]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Figs. 10</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567051372FE34F988FDF9E2D0" box="[436,514,1647,1673]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="4" pageNumber="39">25, 31</figureCitation>
|
||
). This folding is almost certainly a consequence of the extremely broad peristome being compacted into the restricted confines of the peristome proper when the organism contracts. There are three infundibular polykineties, each consisting of three rows of kinetosomes. The adstomal ends of the three rows of infundibular polykinety 1 (P1) terminate at the same level while those of P2 terminate at different levels, the kinetosome row next to P3 being the shortest, and the one next to P1 the longest (
|
||
<figureCitation id="130C2A2567051372FB7AF8E8FA91E370" box="[1274,1386,1807,1833]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Figs. 8, 9</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567051372FAF7F8E8FF43E308" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="4" pageNumber="39">26, 27</figureCitation>
|
||
). The latter sometimes has a short, additional branch (
|
||
<figureCitation id="130C2A2567051372FCC6F8D0FC67E308" box="[838,924,1847,1873]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Figs. 9</figureCitation>
|
||
, arrow, 27, arrow). The row of P3 farthest from P2 (outer row) is shorter than the other two rows (
|
||
<figureCitation id="130C2A2567051372FC9EF8B8FC8BE320" box="[798,880,1887,1913]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Figs. 8</figureCitation>
|
||
, arrowhead, 9, arrowhead). The outer two rows of P3 join together approximately 1/3 of the way from their abstomal to adstomal ends. The inner row of P3 terminates adstomally either at the same level as the outer two (
|
||
<figureCitation id="130C2A2567051372FC0AF848FC2AE390" box="[906,977,1967,1993]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Fig. 8</figureCitation>
|
||
, double arrowhead) or above the outer two at about the same level as the outer row of P2 (
|
||
<figureCitation id="130C2A2567051372FD6FF830FCCDE3A8" box="[751,822,2007,2033]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="4" pageNumber="39">Fig. 9</figureCitation>
|
||
, double arrowhead).
|
||
</paragraph>
|
||
<caption id="DF48662867041373FF17F8ACFE78E3B4" httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="5" pageNumber="40" targetBox="[248,1346,180,1858]" targetPageId="5">
|
||
<paragraph id="8B8836A067041373FF17F8ACFE78E3B4" blockId="5.[151,1436,1867,2030]" pageId="5" pageNumber="40">
|
||
<emphasis id="B943EAB267041373FF17F8ACFEA5E33A" bold="true" box="[151,350,1867,1891]" pageId="5" pageNumber="40">FIGURES 11–21.</emphasis>
|
||
<taxonomicName id="4C374D2367041373FEE6F8ABFDBAE33A" box="[358,577,1868,1891]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="5" pageNumber="40" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
|
||
<emphasis id="B943EAB267041373FEE6F8ABFDBAE33A" box="[358,577,1868,1891]" italics="true" pageId="5" pageNumber="40">Pseudepistylis songi</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A27057C967041373FDCAF8ACFD7CE33A" box="[586,647,1867,1892]" pageId="5" pageNumber="40" rank="species">
|
||
<emphasis id="B943EAB267041373FDCAF8ACFD7CE33A" bold="true" box="[586,647,1867,1892]" pageId="5" pageNumber="40">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20).
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF48662867071370FF17F90CFCFCE3AC" httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="6" pageNumber="41" targetBox="[199,1383,196,1749]" targetPageId="6">
|
||
<paragraph id="8B8836A067071370FF17F90CFCFCE3AC" blockId="6.[151,1436,1771,2038]" pageId="6" pageNumber="41">
|
||
<emphasis id="B943EAB267071370FF17F90CFEA6E35A" bold="true" box="[151,349,1771,1795]" pageId="6" pageNumber="41">FIGURES 22–37.</emphasis>
|
||
<taxonomicName id="4C374D2367071370FEE3F90BFDC7E35A" box="[355,572,1772,1795]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="6" pageNumber="41" phylum="Ciliophora" rank="species" species="songi" status="sp. nov.">
|
||
<emphasis id="B943EAB267071370FEE3F90BFDC7E35A" box="[355,572,1772,1795]" italics="true" pageId="6" pageNumber="41">Pseudepistylis songi</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="B943EAB267071370FDC2F90CFD86E35A" bold="true" box="[578,637,1771,1795]" pageId="6" pageNumber="41">
|
||
<taxonomicNameLabel id="A27057C967071370FDC2F90CFD86E35A" box="[578,637,1771,1795]" pageId="6" pageNumber="41" rank="species">n. sp.</taxonomicNameLabel>
|
||
</emphasis>
|
||
protargol (22–29, 31–37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P1–P3, infundibular polykineties 1–3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B8836A067061371FF46FF7FFEBEE5FB" blockId="7.[151,1437,152,1418]" pageId="7" pageNumber="42">
|
||
The haplokinety passes around the infundibulum on the wall opposite to the infundibular polykineties. The germinal kinety (G) is relatively long, lying adoral to and parallel to the haplokinety and terminates adstomally slightly above the adstomal end of the haplokinety (
|
||
<figureCitation id="130C2A2567061371FC14FF0FFC0CE55B" box="[916,1015,232,258]" captionStart="FIGURES 8 – 10" captionStartId="4.[151,269,1075,1099]" captionTargetBox="[204,1404,200,1067]" captionTargetId="figure@4.[195,1409,194,1071]" captionTargetPageId="4" captionText="FIGURES 8 – 10. Infraciliature of oral apparatus of Pseudepistylis songi n. sp. following protargol impregnation. 8. Typical arrangement of three infundibular polykineties showing the short outer row of P 3 (arrowhead) and the inner row of P 3 (double-arrowhead). 9. An alternative arrangement of the three infundibular polykineties showing the short outer row of P 3 (arrowhead), the shorter inner row of P 3 (double-arrowhead) and the short additional branch of P 2 (arrow). 10. General infraciliature of the oral apparatus. Abbreviations: F, filamentous reticulum; G, germinal kinety; H, haplokinety; P 1 – P 3, infundibular polykineties 1 – 3; Po, polykinety (peristomial continuation of P 1)." httpUri="https://zenodo.org/record/177495/files/figure.png" pageId="7" pageNumber="42">Figs. 10</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FB87FF0FFBADE55B" box="[1031,1110,232,258]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">26, 27</figureCitation>
|
||
). The aboral ciliary wreath (ACW) of the zooid consists of two staggered rows of kinetosomes (dikinety) that encircle the cell at approximately 1/3 of the distance between the scopula and the peristome (
|
||
<figureCitation id="130C2A2567061371FC40FEDFFBEEE50B" box="[960,1045,312,338]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
, arrow, 35, arrow). The ACW is significantly broader in the telotroch because of the proliferation of kinetosomes to form a polykinety (
|
||
<figureCitation id="130C2A2567061371FAE1FE87FF18E5FB" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">Figs. 32, 34</figureCitation>
|
||
, arrow).
|
||
</paragraph>
|
||
<paragraph id="8B8836A067061371FF46FE57FC17E6CB" blockId="7.[151,1437,152,1418]" pageId="7" pageNumber="42">
|
||
The silverline system is reticulate, consisting of both transverse and longitudinal silverlines (
|
||
<figureCitation id="130C2A2567061371FA8DFE57FA9BE593" box="[1293,1376,432,458]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 5</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FAEDFE57FA75E593" box="[1389,1422,432,458]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">30</figureCitation>
|
||
). The transverse silverlines in the oral region of the cell are more widely spaced than those in the aboral region (
|
||
<figureCitation id="130C2A2567061371FF1FFDE7FF11E643" box="[159,234,512,538]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Fig. 5</figureCitation>
|
||
). The ACW is represented by two parallel silverlines (
|
||
<figureCitation id="130C2A2567061371FCFDFDE7FC2AE643" box="[893,977,512,538]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 5</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FC61FDE7FBFAE643" box="[993,1025,512,538]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">30</figureCitation>
|
||
, arrow). There are 125–145 transverse silverlines between the peristome and the ACW and 70–80 between the ACW and the scopula (
|
||
<figureCitation id="130C2A2567061371FAC5FDCFFA76E61B" box="[1349,1421,552,578]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Fig. 5</figureCitation>
|
||
). The vertical silverlines are irregularly spaced (
|
||
<figureCitation id="130C2A2567061371FD44FDB7FCE5E633" box="[708,798,592,618]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">Fig. 30</figureCitation>
|
||
). No pellicular pores associated with silverlines were observed. The scopula is the most argentophilic part of the cell (
|
||
<figureCitation id="130C2A2567061371FC06FD9FFC25E6CB" box="[902,990,632,658]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">Fig. 37</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B8836A067061371FF46FD47FCA3E753" blockId="7.[151,1437,152,1418]" pageId="7" pageNumber="42">
|
||
There are two main kinds of myonemal fibers in
|
||
<emphasis id="B943EAB267061371FC8AFD47FBFAE6E0" box="[778,1025,672,697]" italics="true" pageId="7" pageNumber="42">
|
||
<taxonomicName id="4C374D2367061371FC8AFD47FC07E6E0" box="[778,1020,672,697]" class="Oligohymenophorea" family="Epistylididae" genus="Pseudepistylis" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Peritrichida" pageId="7" pageNumber="42" phylum="Ciliophora" rank="species" species="songi">Pseudepistylis songi</taxonomicName>
|
||
,
|
||
</emphasis>
|
||
the longitudinal fibers and the circular fibers, both of which comprise several morphological subtypes. The longitudinal and circular fibers are interconnected to form a single, complete myoneme system.
|
||
</paragraph>
|
||
<paragraph id="8B8836A067061371FF46FCFFFEA2E02B" blockId="7.[151,1437,152,1418]" pageId="7" pageNumber="42">
|
||
<emphasis id="B943EAB267061371FF46FCFFFE52E768" box="[198,425,792,817]" italics="true" pageId="7" pageNumber="42">Longitudinal fibers</emphasis>
|
||
: Three
|
||
<typeStatus id="548C880267061371FD87FCFFFDBEE76B" box="[519,581,792,818]" pageId="7" pageNumber="42">types</typeStatus>
|
||
of longitudinal fiber can be recognized: long longitudinal fibers (LLF), short longitudinal fibers (SLF) and peristomial longitudinal fibers (PLF) (
|
||
<figureCitation id="130C2A2567061371FC72FCA7FBBFE703" box="[1010,1092,832,858]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FBD0FCA7FB0FE703" box="[1104,1268,832,858]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">22–24, 28, 29</figureCitation>
|
||
). The LLF are less numerous, more diffuse, and more slender than the other two
|
||
<typeStatus id="548C880267061371FC46FC8FFBFFE7DB" box="[966,1028,872,898]" pageId="7" pageNumber="42">types</typeStatus>
|
||
of longitudinal fibers and extend throughout the entire length of the zooid (
|
||
<figureCitation id="130C2A2567061371FD05FC77FD22E7F3" box="[645,729,912,938]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FD66FC77FCCFE7F3" box="[742,820,912,938]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">28, 29</figureCitation>
|
||
). The SLF are the most densely packed and thickest in diameter of the three
|
||
<typeStatus id="548C880267061371FE34FC5FFE09E78B" box="[436,498,952,978]" pageId="7" pageNumber="42">types</typeStatus>
|
||
and are confined to the region between the PLF and the adoral one third of the zooid (
|
||
<figureCitation id="130C2A2567061371FF69FC07FEC6E7A3" box="[233,317,992,1018]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FECBFC07FE97E7A3" box="[331,364,992,1018]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">29</figureCitation>
|
||
). The PLF are also densely packed, being intermediate in thickness between the other two
|
||
<typeStatus id="548C880267061371FF17FBEFFF23E07B" box="[151,216,1032,1058]" pageId="7" pageNumber="42">types</typeStatus>
|
||
, and are confined to the region of the peristomial lip (
|
||
<figureCitation id="130C2A2567061371FCCDFBEFFC5AE07B" box="[845,929,1032,1058]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FC2DFBEFFBDDE07B" box="[941,1062,1032,1058]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">22–24, 29</figureCitation>
|
||
). All three
|
||
<typeStatus id="548C880267061371FB29FBEFFB1CE07B" box="[1193,1255,1032,1058]" pageId="7" pageNumber="42">types</typeStatus>
|
||
of longitudinal fibers branch and interconnect with nearby fibers, thus forming a complete, anastomosing, meshlike network (
|
||
<figureCitation id="130C2A2567061371FF1FFBBFFF08E02B" box="[159,243,1112,1138]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
||
<figureCitation id="130C2A2567061371FF7FFBBFFEB7E02B" box="[255,332,1112,1138]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">28, 29</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B8836A067061371FF46FB67FAB8E1D3" blockId="7.[151,1437,152,1418]" pageId="7" pageNumber="42">
|
||
<emphasis id="B943EAB267061371FF46FB67FE88E0C0" box="[198,371,1152,1177]" italics="true" pageId="7" pageNumber="42">Circular fibers</emphasis>
|
||
: Three
|
||
<typeStatus id="548C880267061371FE4EFB67FDF7E0C3" box="[462,524,1152,1178]" pageId="7" pageNumber="42">types</typeStatus>
|
||
of circular fibers were observed in the oral region of the cell. From outermost to innermost these are: peristomial ring fibers (PRF), central ring fibers (CRF), and peristomial disc fibers (PDF) (
|
||
<figureCitation id="130C2A2567061371FF70FB37FEB9E0B3" box="[240,322,1232,1258]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
||
,
|
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<figureCitation id="130C2A2567061371FECEFB37FE3CE0B3" box="[334,455,1232,1258]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">22–25, 29</figureCitation>
|
||
). The PRF consists of approximately 10 individual circular fibers located within the peristomial lip (
|
||
<figureCitation id="130C2A2567061371FED0FB1FFE59E14B" box="[336,418,1272,1298]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
|
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,
|
||
<figureCitation id="130C2A2567061371FE2EFB1FFDA9E14B" box="[430,594,1272,1298]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">22–24, 29, 36</figureCitation>
|
||
). The CRF comprises several circular fibers that combine to form a conspicuously thickened fiber located in the adoral part of the cell and connecting to the adoral ends of the PLF (
|
||
<figureCitation id="130C2A2567061371FF1FFAAFFF0EE13B" box="[159,245,1352,1378]" captionStart="FIGURES 1 – 7" captionStartId="3.[151,269,1373,1397]" captionTargetBox="[156,1427,203,1345]" captionTargetId="figure@3.[151,1436,197,1346]" captionTargetPageId="3" captionText="FIGURES 1 – 7. Pseudepistylis songi n. sp. in vivo (1 – 4, 6), protargol impregnation (7), silver nitrate impregnation (5). 1. Typical zooid; arrow indicates micronucleus. 2. Telotroch. 3. Colony of eight zooids showing both extended and contracted states as well as structure of stalk. 4. Detached zooid; arrowheads mark pellicular folds. 5. General silverline system; arrows indicate aboral ciliary wreath. 6. Zooids at low magnification, showing varieties of body shape when contracted; arrowheads mark pellicular folds. 7. Myoneme system; arrows indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; LLF, long longitudinal fibers; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 100 μm (Fig. 1), 600 μm (Fig. 3)." httpUri="https://zenodo.org/record/177494/files/figure.png" pageId="7" pageNumber="42">Figs. 7</figureCitation>
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,
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<figureCitation id="130C2A2567061371FE84FAAFFE84E13B" box="[260,383,1352,1378]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">22–25, 29</figureCitation>
|
||
). It appears that contraction of the CRF causes the peristomial disc to elevate when the zooid contracts (
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||
<figureCitation id="130C2A2567061371FED9FA97FE41E1D3" box="[345,442,1392,1418]" captionStart="FIGURES 11 – 21" captionStartId="5.[151,269,1867,1891]" captionTargetBox="[248,1346,180,1858]" captionTargetId="figure@5.[246,1348,180,1861]" captionTargetPageId="5" captionText="FIGURES 11 – 21. Pseudepistylis songi n. sp. in vivo. 11. Typical zooid. 12, 15, 18. Contracted zooid. 13, 14, 16, 17. Detached zooid. 19. Telotroch. 20. Stalk. 21. Newly divided zooids and stalk. Arrow in 11 indicates contractile vacuole; arrowheads in 12 and 14 indicate pellicular folds; arrows in 12 and 15 indicate food vacuoles; arrow in 13 indicates peristomial disc; arrow in 18 indicates macronucleus; arrow in 19 indicates aboral ciliary wreath. Scale bars: 100 μm (Fig. 11), 300 μm (Fig. 20)." httpUri="https://zenodo.org/record/177496/files/figure.png" pageId="7" pageNumber="42">Figs. 13</figureCitation>
|
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,
|
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<figureCitation id="130C2A2567061371FE47FA97FDEFE1D3" box="[455,532,1392,1418]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">24, 25</figureCitation>
|
||
). The PDF are relatively inconspicuous, short, bundles (
|
||
<figureCitation id="130C2A2567061371FB26FA97FACDE1D3" box="[1190,1334,1392,1418]" captionStart="FIGURES 22 – 37" captionStartId="6.[151,269,1771,1795]" captionTargetBox="[199,1383,196,1749]" captionTargetId="figure@6.[197,1384,194,1750]" captionTargetPageId="6" captionText="FIGURES 22 – 37. Pseudepistylis songi n. sp. protargol (22 – 29, 31 – 37) and silver nitrate (30) impregnation. 22, 25. Typical zooid. 23, 24. Peristomial disc and myonemal fibers. 26, 27. Infraciliature of oral apparatus. 28, 29. Myoneme system. 30. Pellicular striae. 31. Haplokinety and polykinety. 32, 34. Aboral ciliary wreath of telotroch. 33. Zooid showing macronucleus and micronucleus (arrow). 35. Aboral ciliary wreath of typical zooid. 36. Oral fiber. 37. Scopula. Arrows in 30, 32, 34, 35 indicate aboral ciliary wreath. Abbreviations: CRF, central ring fibers; F, filamentous reticulum; G, germinal kinety; H, haplokinety; LLF, long longitudinal fibers; P 1 – P 3, infundibular polykineties 1 – 3; PDF, peristomial disc fibers; PLF, peristomial longitudinal fibers; Po, polykinety (peristomial continuation of P 1); PRF, peristomial ring fibers; SLF, short longitudinal fibers. Scale bars: 50 μm (Fig. 25)." httpUri="https://zenodo.org/record/177497/files/figure.png" pageId="7" pageNumber="42">Figs. 23, 24</figureCitation>
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||
).
|
||
</paragraph>
|
||
</subSubSection>
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||
</treatment>
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||
</document> |