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<document id="102DFD4EC5AAEDDFB8D85E7A36002882" ID-CLB-Dataset="97042" ID-DOI="10.5852/ejt.2022.828.1861" ID-GBIF-Dataset="db902fb6-703b-4457-a4e8-37be2a702d74" ID-ISSN="2118-9773" ID-Zenodo-Dep="6839811" ID-ZooBank="92E8908C-818C-49CD-8E84-A95479F8E455" IM.bibliography_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1657891128196" checkinUser="felipe" docAuthor="Robert, Chloé, Pereira, Raquel &amp; Thollesson, Mikael" docDate="2022" docId="90618787FFC4FFFDFDC1FDCC1C9391FE" docLanguage="en" docName="ejt-828_138-167.pdf" docOrigin="European Journal of Taxonomy 828 (1)" docSource="http://dx.doi.org/10.5852/ejt.2022.828.1861" docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1" docStyleId="EF2B578F1D15862ADE45B0C07C620911" docStyleName="EJT.2018-.journal_article.type1" docStyleVersion="14" docTitle="Eunapius fragilis" docType="treatment" docVersion="8" lastPageNumber="159" masterDocId="6C58FFFFFFD6FFE8FF9CFFD31E6D9508" masterDocTitle="Addition to Sweden’s freshwater sponge fauna and a phylogeographic study of Spongilla lacustris (Spongillida, Porifera) in southern Sweden" masterLastPageNumber="167" masterPageNumber="138" pageNumber="156" updateTime="1699359141193" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0" zenodo-license-figures="CC-BY-4.0" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="F5BDF9142E43B9650B3037AB5F944615">Addition to Sweden’s freshwater sponge fauna and a phylogeographic study of Spongilla lacustris (Spongillida, Porifera) in southern Sweden</mods:title>
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<mods:namePart id="1B93B736995FEC19EF61EEDBCE15A740">Robert, Chloé</mods:namePart>
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<mods:affiliation id="7164A50F7AB3D2C2079B5A96216E8B79">Dept. Organismal Biology, Uppsala University, Norbyvägen 18 D, SE- 752 36 Uppsala, Sweden. &amp; Dept. Marine Sciences, University of Gothenburg, Box 461, SE- 405 30 Göteborg, Sweden.</mods:affiliation>
<mods:nameIdentifier id="1A5893952FC9C4A201C7AFC482E10CC4" type="email">chloe.robert@gu.se</mods:nameIdentifier>
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<mods:namePart id="B44B20FDF2885C8E804372F16FE495F9">Pereira, Raquel</mods:namePart>
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<mods:affiliation id="0B9F240CFB92280037612207CBE5F10B">Dept. Organismal Biology, Uppsala University, Norbyvägen 18 D, SE- 752 36 Uppsala, Sweden.</mods:affiliation>
<mods:nameIdentifier id="08090EB0F5EDEE5D08270CEBF167DC8E" type="email">raquel.pereira@ebc.uu.se</mods:nameIdentifier>
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<mods:namePart id="74A5DE523A761E5A76B355E1E4C24211">Thollesson, Mikael</mods:namePart>
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<mods:affiliation id="9E31B2815BB04DD9BEB97A9961ABA574">Dept. Organismal Biology, Uppsala University, Norbyvägen 18 D, SE- 752 36 Uppsala, Sweden.</mods:affiliation>
<mods:nameIdentifier id="8218A54AB33F0DF1CC27B66D1037B257" type="email">mikael.thollesson@ebc.uu.se</mods:nameIdentifier>
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<mods:title id="381E5DC48127BD901A137B536A66264E">European Journal of Taxonomy</mods:title>
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<mods:date id="1BFAE56B782B9CAAC434635A9A94BB6F">2022</mods:date>
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<treatment id="90618787FFC4FFFDFDC1FDCC1C9391FE" ID-DOI="http://doi.org/10.5281/zenodo.6841099" ID-GBIF-Taxon="197571889" ID-Zenodo-Dep="6841099" LSID="urn:lsid:plazi:treatment:90618787FFC4FFFDFDC1FDCC1C9391FE" httpUri="http://treatment.plazi.org/id/90618787FFC4FFFDFDC1FDCC1C9391FE" lastPageId="21" lastPageNumber="159" pageId="18" pageNumber="156">
<subSubSection id="50D2651AFFC4FFFAFDC1FDCC1DBA9732" box="[605,983,542,570]" pageId="18" pageNumber="156" type="nomenclature">
<paragraph id="18773691FFC4FFFAFDC1FDCC1DBA9732" blockId="18.[605,983,542,605]" box="[605,983,542,570]" pageId="18" pageNumber="156">
<heading id="433F81FDFFC4FFFAFDC1FDCC1DBA9732" box="[605,983,542,570]" centered="true" fontSize="11" level="2" pageId="18" pageNumber="156" reason="2">
<taxonomicName id="DFC84D12FFC4FFFAFDC1FDCC1DBA9732" ID-CoL="6GYRV" authority="(Leidy, 1851)" baseAuthorityName="Leidy" baseAuthorityYear="1851" box="[605,983,542,570]" class="Demospongiae" family="Spongillidae" genus="Eunapius" kingdom="Animalia" order="Spongillida" pageId="18" pageNumber="156" phylum="Porifera" rank="species" species="fragilis">
<emphasis id="2ABCEA83FFC4FFFAFDC1FDCC1D5D9731" bold="true" box="[605,816,542,569]" italics="true" pageId="18" pageNumber="156">Eunapius fragilis</emphasis>
(
<bibRefCitation id="7C594B60FFC4FFFAFCA3FDCC1DA39732" author="Leidy J." box="[831,974,543,570]" pageId="18" pageNumber="156" pagination="278" refId="ref13509" refString="Leidy J. 1851. On Spongilla. Proceedings of the Academy of Natural Sciences of Philadelphia 5: 278." type="journal article" year="1851">Leidy, 1851</bibRefCitation>
)
</taxonomicName>
</heading>
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<paragraph id="18773691FFC4FFFAFD6BFD911D519755" blockId="18.[605,983,542,605]" box="[759,828,578,605]" pageId="18" pageNumber="156">
<figureCitation id="80F32A14FFC4FFFAFD6BFD911D519755" box="[759,828,578,605]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="50D2651AFFC4FFFAFF21FD571C0D9796" box="[189,608,643,670]" pageId="18" pageNumber="156" type="reference_group">
<paragraph id="18773691FFC4FFFAFF21FD571C0D9796" blockId="18.[189,608,643,670]" box="[189,608,643,670]" pageId="18" pageNumber="156">
<treatmentCitationGroup id="38D811BFFFC4FFFAFF21FD571C0D9796" box="[189,608,643,670]" pageId="18" pageNumber="156">
<treatmentCitation id="99691080FFC4FFFAFF21FD571C319796" author="Leidy J." box="[189,604,643,670]" page="278" pageId="18" pageNumber="156" year="1851">
<taxonomicName id="DFC84D12FFC4FFFAFF21FD571C319796" ID-CoL="92YRN" authority="Leidy, 1851: 278" authorityName="Leidy" authorityPageNumber="278" authorityYear="1851" box="[189,604,643,670]" class="Demospongiae" family="Spongillidae" genus="Spongilla" kingdom="Animalia" order="Spongillida" pageId="18" pageNumber="156" phylum="Porifera" rank="species" species="fragilis">
<emphasis id="2ABCEA83FFC4FFFAFF21FD571FE79796" box="[189,394,644,670]" italics="true" pageId="18" pageNumber="156">Spongilla fragilis</emphasis>
<bibRefCitation id="7C594B60FFC4FFFAFE0DFD571C319796" author="Leidy J." box="[401,604,643,670]" pageId="18" pageNumber="156" pagination="278" refId="ref13509" refString="Leidy J. 1851. On Spongilla. Proceedings of the Academy of Natural Sciences of Philadelphia 5: 278." type="journal article" year="1851">Leidy, 1851: 278</bibRefCitation>
</taxonomicName>
</treatmentCitation>
.
</treatmentCitationGroup>
</paragraph>
</subSubSection>
<subSubSection id="50D2651AFFC4FFFAFF21FD161A079693" pageId="18" pageNumber="156" type="materials_examined">
<paragraph id="18773691FFC4FFFAFF21FD161CA597D7" blockId="18.[189,712,709,735]" box="[189,712,709,735]" pageId="18" pageNumber="156">
<emphasis id="2ABCEA83FFC4FFFAFF21FD161FC797D7" bold="true" box="[189,426,709,735]" pageId="18" pageNumber="156">Material examined</emphasis>
(
<specimenCount id="0ECEFD18FFC4FFFAFE27FD161C3197D7" box="[443,604,709,735]" pageId="18" pageNumber="156" type="generic">11 specimens</specimenCount>
,
<tableCitation id="554A032AFFC4FFFAFDFBFD161CD197D7" box="[615,700,709,735]" captionStart="Table 1" captionStartId="6.[185,255,547,575]" captionTargetPageId="6" captionText="Table 1 (continued). Material examined in the present study. Abbreviations of Swedish provinces: Bhl = Bohuslän; Dlr = Dalarna; Dls = Dalsland; Hls = Hälsingland; Hrj = Härjedalen; Jmt = Jämtland; Mpd = Medelpad; Sdm = Södermanland; Sth = Stockholm; Upp = Uppland." httpUri="http://table.plazi.org/id/4CB76619FFD0FFEEFF25FDF018DB976A" pageId="18" pageNumber="156" tableUuid="4CB76619FFD0FFEEFF25FDF018DB976A">Table 1</tableCitation>
)
</paragraph>
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<materialsCitation id="A8A03CCCFFC4FFFAFF21FD271D6A9639" ID-GBIF-Occurrence="3863151336" collectingDate="2017-08-02" collectionCode="UPSZMC" collectorName="Chloe Robert &amp; Raquel Pereira" country="Sweden" latitude="59.7636" location="Hammarskogs bath" longLatPrecision="6" longitude="17.5762" pageId="18" pageNumber="156" specimenCode="UPSZMC 188108" specimenCount="1" stateProvince="Uppland">
<collectingCountry id="60DF7601FFC4FFFAFF21FD271F5A9606" box="[189,311,756,782]" name="Sweden" pageId="18" pageNumber="156">SWEDEN</collectingCountry>
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<collectingRegion id="DA0CF873FFC4FFFAFEC0FD271FA49606" box="[348,457,756,782]" pageId="18" pageNumber="156">Uppland</collectingRegion>
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•
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and
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leg.;
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•
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leg.;
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•
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</date>
;
<collectorName id="B53D5347FFC4FFFAFC33FC8E1A22967F" box="[943,1103,861,887]" pageId="18" pageNumber="156">Chloé Robert</collectorName>
and
<collectorName id="B53D5347FFC4FFFAFB17FC8E1B57967F" box="[1163,1338,861,887]" pageId="18" pageNumber="156">Raquel Pereira</collectorName>
leg.;
<specimenCode id="486E9EEAFFC4FFFAFF23FC521FFB9692" box="[191,406,896,923]" pageId="18" pageNumber="156">UPSZMC 188163</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFE3EFC531F909693" box="[418,509,896,923]" pageId="18" pageNumber="156">188215</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFD95FC521C099693" box="[521,612,897,923]" pageId="18" pageNumber="156">188217</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFDF3FC521CA79693" box="[623,714,897,923]" pageId="18" pageNumber="156">188219</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFD4AFC521D5C9693" box="[726,817,897,923]" pageId="18" pageNumber="156">188221</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFCA1FC521DF59693" box="[829,920,897,923]" pageId="18" pageNumber="156">188223</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFC3FFC531D939693" box="[931,1022,896,923]" pageId="18" pageNumber="156">188225</specimenCode>
,
<specimenCode id="486E9EEAFFC4FFFAFB96FC521A0B9693" box="[1034,1126,897,923]" pageId="18" pageNumber="156">188227</specimenCode>
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="50D2651AFFC4FFFAFF21FC111F4D91F4" pageId="18" pageNumber="156" type="description">
<paragraph id="18773691FFC4FFFAFF21FC111F2396D4" blockId="18.[189,334,962,988]" box="[189,334,962,988]" pageId="18" pageNumber="156">
<heading id="433F81FDFFC4FFFAFF21FC111F2396D4" bold="true" box="[189,334,962,988]" fontSize="11" level="3" pageId="18" pageNumber="156" reason="3">
<emphasis id="2ABCEA83FFC4FFFAFF21FC111F2396D4" bold="true" box="[189,334,962,988]" pageId="18" pageNumber="156">Description</emphasis>
</heading>
</paragraph>
<paragraph id="18773691FFC4FFFAFF21FC221F5C9126" blockId="18.[188,1399,1008,1070]" pageId="18" pageNumber="156">
HABITUS. Specimens of this species have a pale green colour, slightly lighter than the other three species (
<figureCitation id="80F32A14FFC4FFFAFF59FBC01F499126" box="[197,292,1043,1070]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5A</figureCitation>
).
</paragraph>
<paragraph id="18773691FFC4FFFAFF21FB861F4D91F4" blockId="18.[188,1399,1109,1277]" pageId="18" pageNumber="156">
SPICULAE. Megascleres and gemmuloscleres. Megascleres are smooth and fusiform oxeas (
<figureCitation id="80F32A14FFC4FFFAFA94FB861B049178" box="[1288,1385,1109,1136]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5B</figureCitation>
). Gemmuloscleres are spiny and rod-shaped (
<figureCitation id="80F32A14FFC4FFFAFD23FBAB1D76919B" box="[703,795,1144,1171]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5B</figureCitation>
). Measured megascleres 219.5 μm (110 to 300 μm) long (
<figureCitation id="80F32A14FFC4FFFAFE9CFB481F3791BE" box="[256,346,1179,1206]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5C</figureCitation>
) and 6.7 µm (2.5 to 10 μm) wide. Few gemmuloscleres were present,
<quantity id="DF309B74FFC4FFFAFB14FB4F1AAE91BD" box="[1160,1219,1179,1206]" metricMagnitude="0" metricUnit="m" metricValue="1.2446" pageId="18" pageNumber="156" unit="in" value="49.0">49 in</quantity>
<specimenCount id="0ECEFD18FFC4FFFAFB56FB4F1B1B91BD" box="[1226,1398,1179,1206]" pageId="18" pageNumber="156" type="generic">two specimens</specimenCount>
were measured. Their average length was 85.5 μm (60 to 110 μm) (
<figureCitation id="80F32A14FFC4FFFAFC40FB6C1A5591D1" box="[988,1080,1214,1241]" captionStart="Fig" captionStartId="19.[188,231,1812,1838]" captionTargetBox="[189,1395,703,1750]" captionTargetId="figure-134@19.[186,1401,701,1778]" captionTargetPageId="19" captionText="Fig. 5. Eunapius fragilis (Leidy, 1851). A. Habitus (P059-170829-4). B. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1). C–D. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured. C. Megascleres. Brackets correspond to ranges in literature (red = Tendal 1967b; blue = Penney &amp; Racek 1968; black = Evans &amp; Montagnes 2019). D. Gemmuloscleres." figureDoi="http://doi.org/10.5281/zenodo.6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="18" pageNumber="156">Fig. 5D</figureCitation>
) and width 5.6 μm, (2.5 to 7.5 μm).
</paragraph>
</subSubSection>
<subSubSection id="50D2651AFFC4FFFAFF21FAF01D349087" pageId="18" pageNumber="156" type="distribution">
<paragraph id="18773691FFC4FFFAFF21FAF01F949035" blockId="18.[189,505,1315,1341]" box="[189,505,1315,1341]" pageId="18" pageNumber="156">
<heading id="433F81FDFFC4FFFAFF21FAF01F949035" bold="true" box="[189,505,1315,1341]" fontSize="11" level="3" pageId="18" pageNumber="156" reason="3">
<emphasis id="2ABCEA83FFC4FFFAFF21FAF01F949035" bold="true" box="[189,505,1315,1341]" pageId="18" pageNumber="156">Distribution and habitats</emphasis>
</heading>
</paragraph>
<paragraph id="18773691FFC4FFFAFF21FA811D349087" blockId="18.[188,1399,1362,1424]" pageId="18" pageNumber="156">
Specimens collected in southeast and northern part of sampling area (
<figureCitation id="80F32A14FFC4FFFAFC7BFA811A469064" box="[999,1067,1362,1388]" captionStart="Fig" captionStartId="3.[189,232,1779,1805]" captionTargetBox="[203,1384,265,1741]" captionTargetId="figure-13@3.[203,1384,265,1741]" captionTargetPageId="3" captionText="Fig. 1. Sampling sites and species found in main catchment areas in Sweden. Black lines represent watersheds between catchment areas, from the Swedish Meteorological and Hydrological Institute SVAR database (Westman et al. 2017). Crosses are sites visited but where no sponge was found, dots are sites where sponges were collected. The neighbouring pie icon indicates the species present at that site: blue (top right) is Spongilla lacustris (Linnaeus, 1759), yellow (bottom right) Eunapius fragilis (Leidy, 1851), black (bottom left) Ephydatia fluviatilis (Linnaeus, 1759) and green (top left) Ephydatia muelleri (Lieberkühn, 1856)." figureDoi="http://doi.org/10.5281/zenodo.6839813" httpUri="https://zenodo.org/record/6839813/files/figure.png" pageId="18" pageNumber="156">Fig. 1</figureCitation>
); no specimens found in the western part; from lakes and in a river close to lake inlet.
</paragraph>
</subSubSection>
<subSubSection id="50D2651AFFC4FFFDFF21FA641C9391FE" lastPageId="21" lastPageNumber="159" pageId="18" pageNumber="156" type="description">
<paragraph id="18773691FFC4FFFAFF21FA641FC390DC" blockId="18.[189,430,1463,1492]" box="[189,430,1463,1492]" pageId="18" pageNumber="156">
<emphasis id="2ABCEA83FFC4FFFAFF21FA641FC390DC" bold="true" box="[189,430,1463,1492]" italics="true" pageId="18" pageNumber="156">Bar-code analyses</emphasis>
</paragraph>
<paragraph id="18773691FFC4FFFAFF21FA3F1FA893BE" blockId="18.[187,1399,1516,1718]" pageId="18" pageNumber="156">
There was a high within-species similarity in the specimens of the present study, and they fall well within the variation encountered in published sequences. Particularly
<emphasis id="2ABCEA83FFC4FFFAFC32F9C31DBA9321" box="[942,983,1552,1577]" italics="true" pageId="18" pageNumber="156">cox</emphasis>
I sequences were useful (
<figureCitation id="80F32A14FFC4FFFAFA94F9DC1B049321" box="[1288,1385,1551,1577]" captionStart="Fig" captionStartId="20.[188,231,1921,1947]" captionTargetBox="[199,1363,918,1855]" captionTargetId="figure-224@20.[189,1398,908,1883]" captionTargetPageId="20" captionText="Fig. 6. Neighbor-joining trees based on HKY85 distances. A. coxI dataset. B. 28S dataset. Sequences with a specimen field number (starting with P052- or P059-) are from the present study, remaining sequences are from GenBank with the accession number in the label." figureDoi="http://doi.org/10.5281/zenodo.6839829" httpUri="https://zenodo.org/record/6839829/files/figure.png" pageId="18" pageNumber="156">Fig. 6A</figureCitation>
), whereas the use of 28S (
<figureCitation id="80F32A14FFC4FFFAFE41F9E11C5A9344" box="[477,567,1586,1612]" captionStart="Fig" captionStartId="20.[188,231,1921,1947]" captionTargetBox="[199,1363,918,1855]" captionTargetId="figure-224@20.[189,1398,908,1883]" captionTargetPageId="20" captionText="Fig. 6. Neighbor-joining trees based on HKY85 distances. A. coxI dataset. B. 28S dataset. Sequences with a specimen field number (starting with P052- or P059-) are from the present study, remaining sequences are from GenBank with the accession number in the label." figureDoi="http://doi.org/10.5281/zenodo.6839829" httpUri="https://zenodo.org/record/6839829/files/figure.png" pageId="18" pageNumber="156">Fig. 6B</figureCitation>
) was hampered by fewer available sequences in the databases and that the available sequences in many cases were short; of the ca 660 bp amplified by the used primers, there was only ca 350 bp of useful overlap with most GenBank sequences. Both markers provided useful data to identify the species.
</paragraph>
<paragraph id="18773691FFC4FFFAFF21F90D1F1A93F0" blockId="18.[189,375,1758,1784]" box="[189,375,1758,1784]" pageId="18" pageNumber="156">
<heading id="433F81FDFFC4FFFAFF21F90D1F1A93F0" bold="true" box="[189,375,1758,1784]" fontSize="11" level="3" pageId="18" pageNumber="156" reason="3">
<emphasis id="2ABCEA83FFC4FFFAFF21F90D1F1A93F0" bold="true" box="[189,375,1758,1784]" pageId="18" pageNumber="156">Marker search</emphasis>
</heading>
</paragraph>
<paragraph id="18773691FFC4FFFBFF21F8DF1A40944E" blockId="18.[189,1399,1804,2006]" lastBlockId="19.[189,1399,265,327]" lastPageId="19" lastPageNumber="157" pageId="18" pageNumber="156">
Primer pairs tested for amplification are listed in Supp. file 1, but most of them failed to amplify
<taxonomicName id="DFC84D12FFC4FFFAFF21F8FC1FEB9241" authority="DNA" authorityName="DNA" box="[189,390,1839,1865]" class="Demospongiae" family="Spongillidae" genus="Spongilla" kingdom="Animalia" order="Spongillida" pageId="18" pageNumber="156" phylum="Porifera" rank="species" species="lacustris">
<emphasis id="2ABCEA83FFC4FFFAFF21F8FC1F2D9241" box="[189,320,1839,1865]" italics="true" pageId="18" pageNumber="156">S. lacustris</emphasis>
DNA
</taxonomicName>
extracts. Different annealing temperatures and MgCl
<subScript id="844C34D4FFC4FFFAFC66F8911A6E9258" attach="left" box="[1018,1027,1858,1872]" fontSize="6" pageId="18" pageNumber="156">2</subScript>
concentrations were used, with the latter having no effect on the amplification results. No product was detected for 21 of the 24 primer pairs tested. The remaining three primer pairs were successful; i56-F/i56-R, i56-Spla-F/i56-Spla-R and ATP6porF/ATP6porR (primer pairs 23, 25 and
<quantity id="DF309B74FFC4FFFAFD7BF84A1D7892BB" box="[743,789,1945,1972]" metricMagnitude="-1" metricUnit="m" metricValue="1.27" pageId="18" pageNumber="156" unit="in" value="5.0">5 in</quantity>
Supp. file 1). The marker i56 is an intron located in a conserved gene coding for glutamyl-prolyl-tRNA-synthetase (
<bibRefCitation id="7C594B60FFC4FFFAFC33F86F1AF792DE" author="Chenuil A. &amp; Hoareau T. B. &amp; Egea E. &amp; Penant G. &amp; Rocher C. &amp; Aurelle D. &amp; Mokhtar-Jamai K. &amp; Bishop J. D. D. &amp; Boissin E. &amp; Diaz A. &amp; Krakau M. &amp; Luttikhuizen P. C. &amp; Patti F. P. &amp; Blavet N. &amp; Mousset S." box="[943,1178,1980,2006]" pageId="18" pageNumber="156" pagination="276" refId="ref12416" refString="Chenuil A., Hoareau T. B., Egea E., Penant G., Rocher C., Aurelle D., Mokhtar-Jamai K., Bishop J. D. D., Boissin E., Diaz A., Krakau M., Luttikhuizen P. C., Patti F. P., Blavet N. &amp; Mousset S. 2010. An efficient method to find potentially universal population genetic markers, applied to metazoans. BMC Evolutionary Biology 10 (1): e 276. https: // doi. org / 10.1186 / 1471 - 2148 - 10 - 276" type="journal article" year="2010">
Chenuil
<emphasis id="2ABCEA83FFC4FFFAFB8BF86E1A3E92DE" box="[1047,1107,1980,2006]" italics="true" pageId="18" pageNumber="156">et al.</emphasis>
2010
</bibRefCitation>
). Using the primer pair i56-F/i56-R at an annealing temperature of 64°C (following tests of optimal temperature) product was obtained for 43 samples, and 40 of these were successfully sequenced.
</paragraph>
<paragraph id="18773691FFC5FFFBFF21FEBD1B3694C6" blockId="19.[189,1398,365,463]" pageId="19" pageNumber="157">The primer pair ATP6porF/ATP6porR, targeting the mitochondrial gene ATP6, amplified a region ca 450 bp long. However, as expected, a low amount of variation was detected between specimens, with less than 0.7% nucleotide variation. Thus, for this study, only primer pair i56-F / i56-R was selected.</paragraph>
<paragraph id="18773691FFC5FFFBFF21FE261FFC9707" blockId="19.[189,401,501,527]" box="[189,401,501,527]" pageId="19" pageNumber="157">
<heading id="433F81FDFFC5FFFBFF21FE261FFC9707" bold="true" box="[189,401,501,527]" fontSize="11" level="3" pageId="19" pageNumber="157" reason="3">
<emphasis id="2ABCEA83FFC5FFFBFF21FE261FFC9707" bold="true" box="[189,401,501,527]" pageId="19" pageNumber="157">Genetic diversity</emphasis>
</heading>
</paragraph>
<paragraph id="18773691FFC5FFFCFF21FDF61AF79711" blockId="19.[189,1401,548,645]" lastBlockId="20.[188,1400,265,538]" lastPageId="20" lastPageNumber="158" pageId="19" pageNumber="157">
For the i56 marker we found seven distinct sequence variants in the sampled
<taxonomicName id="DFC84D12FFC5FFFBFBC1FDF71A8C9736" box="[1117,1249,548,574]" class="Demospongiae" family="Spongillidae" genus="Spongilla" kingdom="Animalia" order="Spongillida" pageId="19" pageNumber="157" phylum="Porifera" rank="species" species="lacustris">
<emphasis id="2ABCEA83FFC5FFFBFBC1FDF71A8C9736" box="[1117,1249,548,574]" italics="true" pageId="19" pageNumber="157">S. lacustris</emphasis>
</taxonomicName>
populations, which we designate y-24G, y-290G, y-290A, z- 242T, z-242G, w-377G and w-377T (see
<tableCitation id="554A032AFFC5FFFBFB40FD941B589769" box="[1244,1333,583,609]" captionStart="Table 3" captionStartId="21.[191,261,265,291]" captionTargetPageId="21" captionText="Table 3.Variable sites in the 460 bp long alignment of i56 sequences from Spongilla lacustris (Linnaeus, 1759). Seven varieties, designated z-242T, z-242G, y-290G, y-290A, y-24G, w-377G and w-377T, are found, with a total of 27 variable sites in the alignment. A dot indicates identity with the reference sequence (z-242T), a dash indicates an indel, and N is the number of specimens found of this variety." httpUri="http://table.plazi.org/id/4CB76619FFC3FFFDFF23FEDA1B329484" pageId="19" pageNumber="157" tableUuid="4CB76619FFC3FFFDFF23FEDA1B329484">Table 3</tableCitation>
). The most abundant variant was y-290G, observed for 18 individuals collected in six different catchment areas. Variants w-377G and z-242T were also common; 11 and
<specimenCount id="0ECEFD18FFC2FFFCFCD0FEDA1DB0942B" box="[844,989,265,291]" pageId="20" pageNumber="158" type="generic">7 specimens</specimenCount>
, respectively, shared these variants, sampled from five and three different places. Remaining variants (y-24G, y-290A, w-377T, z-242G) were found only for one or
<specimenCount id="0ECEFD18FFC2FFFCFE5AFE831C159461" box="[454,632,335,362]" pageId="20" pageNumber="158" type="generic">two specimens</specimenCount>
. Two specimens from the Ljusnan catchment area shared variant y-290A, one individual from Dalälven had variant z-242G, one from Göta Älv had w-377T, and a single individual from the Svärtaån catchment area was found with y-24G. Genetic distance and raw differences between variants are shown in
<tableCitation id="554A032AFFC2FFFCFD81FE6A1C1894DB" box="[541,629,441,467]" captionStart="Table 4" captionStartId="23.[189,259,265,291]" captionTargetPageId="23" captionText="Table 4. Differences below diagonal, counting gaps as fifth state, and genetic distances (HKY85) as percentages above diagonal, between the different i56 varieties." httpUri="http://table.plazi.org/id/4CB76619FFC1FFFFFF21FEDA1DC5944E" pageId="20" pageNumber="158" tableUuid="4CB76619FFC1FFFFFF21FEDA1DC5944E">Table 4</tableCitation>
. Variants y-290A and w-377T are least similar, with 24 segregating sites and a 2.7% HKY85 nucleotide difference. Least difference distance is found between y-290G and y-290A, z-242T and z-242G, and w-377G and w-377T with just one segregating site.
</paragraph>
<caption id="4CB76619FFC5FFFBFF20F8C71C6C92D7" ID-DOI="http://doi.org/10.5281/zenodo.6839827" ID-Zenodo-Dep="6839827" httpUri="https://zenodo.org/record/6839827/files/figure.png" pageId="19" pageNumber="157" startId="19.[188,231,1812,1838]" targetBox="[189,1395,703,1750]" targetPageId="19">
<paragraph id="18773691FFC5FFFBFF20F8C71C6C92D7" blockId="19.[188,1400,1812,2015]" pageId="19" pageNumber="157">
<emphasis id="2ABCEA83FFC5FFFBFF20F8C71F639227" bold="true" box="[188,270,1812,1839]" pageId="19" pageNumber="157">Fig. 5.</emphasis>
<taxonomicName id="DFC84D12FFC5FFFBFE87F8C61CF49227" authority="(Leidy, 1851)" baseAuthorityName="Leidy" baseAuthorityYear="1851" box="[283,665,1812,1839]" class="Demospongiae" family="Spongillidae" genus="Eunapius" kingdom="Animalia" order="Spongillida" pageId="19" pageNumber="157" phylum="Porifera" rank="species" species="fragilis">
<emphasis id="2ABCEA83FFC5FFFBFE87F8C61F879227" box="[283,490,1813,1839]" italics="true" pageId="19" pageNumber="157">Eunapius fragilis</emphasis>
(
<bibRefCitation id="7C594B60FFC5FFFBFE61F8C61CFD9227" author="Leidy J." box="[509,656,1812,1839]" pageId="19" pageNumber="157" pagination="278" refId="ref13509" refString="Leidy J. 1851. On Spongilla. Proceedings of the Academy of Natural Sciences of Philadelphia 5: 278." type="journal article" year="1851">Leidy, 1851</bibRefCitation>
)
</taxonomicName>
.
<emphasis id="2ABCEA83FFC5FFFBFD37F8C71CAD9226" bold="true" box="[683,704,1812,1838]" pageId="19" pageNumber="157">A</emphasis>
. Habitus (P059-170829-4).
<emphasis id="2ABCEA83FFC5FFFBFB8AF8C61A479227" bold="true" box="[1046,1066,1813,1839]" pageId="19" pageNumber="157">B</emphasis>
. Spiculae, m = megasclere (P059-170830-13), g = gemmulosclere (P059-170902-1).
<emphasis id="2ABCEA83FFC5FFFBFC1FF8EB1DD1925A" bold="true" box="[899,956,1848,1874]" pageId="19" pageNumber="157">C–D</emphasis>
. Estimated spicula size distribution within and between specimens, P059-170802-10 (solid line), P059-170830-2 (dashed) and P059-170830- 4 (dotted). Marks on the x-axis represent spiculae measured.
<emphasis id="2ABCEA83FFC5FFFBFC01F8AD1DDD9290" bold="true" box="[925,944,1918,1944]" pageId="19" pageNumber="157">C</emphasis>
. Megascleres. Brackets correspond to ranges in literature (red =
<bibRefCitation id="7C594B60FFC5FFFBFE66F8721CCD92B3" author="Tendal O. S." box="[506,672,1953,1979]" pageId="19" pageNumber="157" pagination="63 - 67" refId="ref15050" refString="Tendal O. S. 1967 b. Ferskvandsvampe (Spongillidae) i Thy. Flora og Fauna 73 (2): 63 - 67." type="journal article" year="1967">Tendal 1967b</bibRefCitation>
; blue =
<bibRefCitation id="7C594B60FFC5FFFBFC9FF8711A7992B3" author="Penney J. T. &amp; Racek A." box="[771,1044,1953,1980]" pageId="19" pageNumber="157" pagination="1 - 184" refId="ref14391" refString="Penney J. T. &amp; Racek A. 1968. Comprehensive revision of a worldwide collection of freshwater sponges (Porifera, Spongillidae). Bulletin of the United States National Museum 272: 1 - 184. https: // doi. org / 10.5479 / si. 03629236.272.1" type="journal article" year="1968">Penney &amp; Racek 1968</bibRefCitation>
; black =
<bibRefCitation id="7C594B60FFC5FFFBFB19F8711E9292D7" author="Evans K. L. &amp; Montagnes D. J. S." pageId="19" pageNumber="157" pagination="440" refId="ref12927" refString="Evans K. L. &amp; Montagnes D. J. S. 2019. Freshwater sponge (Porifera: Spongillidae) distribution across a landscape: environmental tolerances, habitats, and morphological variation. Invertebrate Biology 138 (3): e 440. https: // doi. org / 10.1111 / ivb. 12258" type="journal article" year="2019">Evans &amp; Montagnes 2019</bibRefCitation>
).
<emphasis id="2ABCEA83FFC5FFFBFE8CF8161F4892D7" bold="true" box="[272,293,1989,2015]" pageId="19" pageNumber="157">D</emphasis>
. Gemmuloscleres.
</paragraph>
</caption>
<paragraph id="18773691FFC2FFFCFF21FD931FA19753" blockId="20.[189,460,576,603]" box="[189,460,576,603]" pageId="20" pageNumber="158">
<heading id="433F81FDFFC2FFFCFF21FD931FA19753" bold="true" box="[189,460,576,603]" fontSize="11" level="3" pageId="20" pageNumber="158" reason="3">
<emphasis id="2ABCEA83FFC2FFFCFF21FD931FA19753" bold="true" box="[189,460,576,603]" pageId="20" pageNumber="158">Phylogenetic network</emphasis>
</heading>
</paragraph>
<paragraph id="18773691FFC2FFFCFF21FDA31CC297A5" blockId="20.[189,1398,623,685]" pageId="20" pageNumber="158">
In a phylogenetic network we see three main clusters; y-24G and y-290G with y-290A, w-377G with w-377T, and z-242T with z-242G (
<figureCitation id="80F32A14FFC2FFFCFDC5FD411CCD97A5" box="[601,672,658,685]" captionStart="Fig" captionStartId="22.[189,232,1814,1840]" captionTargetBox="[201,1323,761,1737]" captionTargetId="figure-122@22.[189,1398,750,1775]" captionTargetPageId="22" captionText="Fig. 7. Networks of i56 varieties in Swedish Spongilla lacustris (Linnaeus, 1759). Colours indicate catchment area. Seven varieties are designated; z-242T, z-242G, t-24G, y-290G, y-290A, w-377G and w-377T, see Table 3. A. PopART integer neighbor-joining network (described in Leigh &amp; Bryant 2015). Pie chart size proportional to the number of specimens found of the variety. Hatches indicate number of substitutions between varieties, sites with indels are excluded; thus, no differences are picked up between z-242T and z-242G. B. SplitsTree neighbor network based on HKY85 distances." figureDoi="http://doi.org/10.5281/zenodo.6839833" httpUri="https://zenodo.org/record/6839833/files/figure.png" pageId="20" pageNumber="158">Fig. 7</figureCitation>
).
</paragraph>
<paragraph id="18773691FFC2FFFCFF21FD071B1B963C" blockId="20.[189,1400,724,821]" pageId="20" pageNumber="158">
As PopArt does not handle gapped sites, parts of the alignment containing gaps were thus ignored in the analysis. Thus, in the median joining network, variants z-242T and z-242G are seen as identical (
<figureCitation id="80F32A14FFC2FFFCFF5AFCC91F4C963C" box="[198,289,794,820]" captionStart="Fig" captionStartId="22.[189,232,1814,1840]" captionTargetBox="[201,1323,761,1737]" captionTargetId="figure-122@22.[189,1398,750,1775]" captionTargetPageId="22" captionText="Fig. 7. Networks of i56 varieties in Swedish Spongilla lacustris (Linnaeus, 1759). Colours indicate catchment area. Seven varieties are designated; z-242T, z-242G, t-24G, y-290G, y-290A, w-377G and w-377T, see Table 3. A. PopART integer neighbor-joining network (described in Leigh &amp; Bryant 2015). Pie chart size proportional to the number of specimens found of the variety. Hatches indicate number of substitutions between varieties, sites with indels are excluded; thus, no differences are picked up between z-242T and z-242G. B. SplitsTree neighbor network based on HKY85 distances." figureDoi="http://doi.org/10.5281/zenodo.6839833" httpUri="https://zenodo.org/record/6839833/files/figure.png" pageId="20" pageNumber="158">Fig. 7A</figureCitation>
) as they differ only in indel sites. In the majority of catchment areas (Svärtaån, Norrström, Göta
</paragraph>
<caption id="4CB76619FFC2FFFCFF20F8521D8B92E9" ID-DOI="http://doi.org/10.5281/zenodo.6839829" ID-Zenodo-Dep="6839829" httpUri="https://zenodo.org/record/6839829/files/figure.png" pageId="20" pageNumber="158" startId="20.[188,231,1921,1947]" targetBox="[199,1363,918,1855]" targetPageId="20">
<paragraph id="18773691FFC2FFFCFF20F8521D8B92E9" blockId="20.[188,1400,1921,2018]" pageId="20" pageNumber="158">
<emphasis id="2ABCEA83FFC2FFFCFF20F8521F669293" bold="true" box="[188,267,1921,1947]" pageId="20" pageNumber="158">Fig. 6.</emphasis>
Neighbor-joining trees based on HKY85 distances.
<emphasis id="2ABCEA83FFC2FFFCFCE2F8521DFF9293" bold="true" box="[894,914,1921,1947]" pageId="20" pageNumber="158">A</emphasis>
.
<emphasis id="2ABCEA83FFC2FFFCFC3EF8511DB99293" box="[930,980,1922,1947]" italics="true" pageId="20" pageNumber="158">coxI</emphasis>
dataset.
<emphasis id="2ABCEA83FFC2FFFCFBDCF8521A3F9293" bold="true" box="[1088,1106,1921,1947]" pageId="20" pageNumber="158">B</emphasis>
. 28S dataset. Sequences with a specimen field number (starting with P052- or P059-) are from the present study, remaining sequences are from GenBank with the accession number in the label.
</paragraph>
</caption>
<caption id="4CB76619FFC3FFFDFF23FEDA1B329484" ID-Table-UUID="4CB76619FFC3FFFDFF23FEDA1B329484" httpUri="http://table.plazi.org/id/4CB76619FFC3FFFDFF23FEDA1B329484" pageId="21" pageNumber="159" startId="21.[191,261,265,291]" targetBox="[191,1399,450,822]" targetIsTable="true" targetPageId="21">
<paragraph id="18773691FFC3FFFDFF23FEDA1B329484" blockId="21.[189,1399,265,397]" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFF23FEDA1F4C942B" bold="true" box="[191,289,265,291]" pageId="21" pageNumber="159">Table 3.</emphasis>
Variable sites in the 460 bp long alignment of i56 sequences from
<taxonomicName id="DFC84D12FFC3FFFDFB8BFEDA1F6C944F" authority="(Linnaeus, 1759)" baseAuthorityName="Linnaeus" baseAuthorityYear="1759" class="Demospongiae" family="Spongillidae" genus="Spongilla" kingdom="Animalia" order="Spongillida" pageId="21" pageNumber="159" phylum="Porifera" rank="species" species="lacustris">
<emphasis id="2ABCEA83FFC3FFFDFB8BFEDA1A9F942B" box="[1047,1266,265,291]" italics="true" pageId="21" pageNumber="159">Spongilla lacustris</emphasis>
(
<bibRefCitation id="7C594B60FFC3FFFDFA9CFEDA1E94944F" author="Linnaeus C." pageId="21" pageNumber="159" refId="ref13604" refString="Linnaeus C. 1759. Systema Naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Editio decima, reformata. Vol. 2. Laurentii Salvii, Stockholm [Holmiae]. https: // doi. org / 10.5962 / bhl. title. 542" type="book" year="1759">Linnaeus, 1759</bibRefCitation>
)
</taxonomicName>
. Seven varieties, designated z-242T, z-242G, y-290G, y-290A, y-24G, w-377G and w-377T, are found, with a total of 27 variable sites in the alignment. A dot indicates identity with the reference sequence (z-242T), a dash indicates an indel, and N is the number of specimens found of this variety.
</paragraph>
</caption>
<paragraph id="18773691FFC3FFFDFEA6FE171B1D963E" pageId="21" pageNumber="159">
<table id="6AC8C431FFC30017FF23FE111B1A963E" box="[191,1399,450,822]" gridcols="18" gridrows="8" pageId="21" pageNumber="159">
<tr id="A6F834D3FFC30017FF23FE111B1A94D1" box="[191,1399,450,473]" gridrow="0" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FE111B1A94D1" box="[191,1399,450,473]" colspan="18" colspanRight="17" gridcol="0" gridrow="0" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFEA6FE171B3E94DF" bold="true" box="[314,1363,452,471]" pageId="21" pageNumber="159">10 24 52 57 60 61 62 63 64 65 66 71 91 128 134 155 182 197 239 240 241 242 271 281 290 377 392</emphasis>
<emphasis id="2ABCEA83FFC3FFFDFAFCFE111B1E94D1" bold="true" box="[1376,1395,450,473]" italics="true" pageId="21" pageNumber="159">N</emphasis>
</th>
</tr>
<tr id="A6F834D3FFC30017FF23FE3F1B1A970E" box="[191,1399,492,518]" gridrow="1" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FE3F1F72970E" box="[191,287,492,518]" gridcol="0" gridrow="1" pageId="21" pageNumber="159">z-242T</th>
<td id="E5295DAFFFC30017FEA0FE3F1F23970E" box="[316,334,492,518]" gridcol="1" gridrow="1" pageId="21" pageNumber="159">C</td>
<td id="E5295DAFFFC30017FEFDFE3F1C19970E" box="[353,628,492,518]" gridcol="2" gridrow="1" pageId="21" pageNumber="159">A C A T C C A A T</td>
<td id="E5295DAFFFC30017FD1BFE3F1CB3970E" box="[647,734,492,518]" gridcol="3" gridrow="1" pageId="21" pageNumber="159">G C T</td>
<td id="E5295DAFFFC30017FD6FFE3F1D6B970E" box="[755,774,492,518]" gridcol="4" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FCBCFE3F1D59970E" box="[800,820,492,518]" gridcol="5" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FCD1FE3F1D0D970E" box="[845,864,492,518]" gridcol="6" gridrow="1" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FCE6FE3F1DE1970E" box="[890,908,492,518]" gridcol="7" gridrow="1" pageId="21" pageNumber="159">C</td>
<td id="E5295DAFFFC30017FC3AFE3F1DD7970E" box="[934,954,492,518]" gridcol="8" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FC4FFE3F1D8B970E" box="[979,998,492,518]" gridcol="9" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FB9CFE3F1A7E970E" box="[1024,1043,492,518]" gridcol="10" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FBB1FE3F1A2D970E" box="[1069,1088,492,518]" gridcol="11" gridrow="1" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FBC6FE3F1A00970E" box="[1114,1133,492,518]" gridcol="12" gridrow="1" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FB1AFE3F1AF7970E" box="[1158,1178,492,518]" gridcol="13" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FB28FE3F1AAA970E" box="[1204,1223,492,518]" gridcol="14" gridrow="1" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFB2AFE3D1AAE970E" bold="true" box="[1206,1219,494,518]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FB7CFE3F1A99970E" box="[1248,1268,492,518]" gridcol="15" gridrow="1" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FA91FE3F1B4D970E" box="[1293,1312,492,518]" gridcol="16" gridrow="1" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FAA6FE3F1B1A970E" box="[1338,1399,492,518]" gridcol="17" gridrow="1" pageId="21" pageNumber="159">
C
<emphasis id="2ABCEA83FFC3FFFDFAFFFE3D1B1D970D" box="[1379,1392,494,517]" italics="true" pageId="21" pageNumber="159">7</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FDCC1B1A9731" box="[191,1399,543,569]" gridrow="2" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FDCC1F729731" box="[191,287,543,569]" gridcol="0" gridrow="2" pageId="21" pageNumber="159">z-242G</th>
<td id="E5295DAFFFC30017FEA0FDCC1F239731" box="[316,334,543,569]" gridcol="1" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FEFDFDCC1C199731" box="[353,628,543,569]" gridcol="2" gridrow="2" pageId="21" pageNumber="159">.........</td>
<td id="E5295DAFFFC30017FD1BFDCC1CB39731" box="[647,734,543,569]" gridcol="3" gridrow="2" pageId="21" pageNumber="159">...</td>
<td id="E5295DAFFFC30017FD6FFDCC1D6B9731" box="[755,774,543,569]" gridcol="4" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCBCFDCC1D599731" box="[800,820,543,569]" gridcol="5" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCD1FDCC1D0D9731" box="[845,864,543,569]" gridcol="6" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCE6FDCC1DE19731" box="[890,908,543,569]" gridcol="7" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC3AFDCC1DD79731" box="[934,954,543,569]" gridcol="8" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC4FFDCC1D8B9731" box="[979,998,543,569]" gridcol="9" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB9CFDCC1A7E9731" box="[1024,1043,543,569]" gridcol="10" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBB1FDCC1A2D9731" box="[1069,1088,543,569]" gridcol="11" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBC6FDCC1A009731" box="[1114,1133,543,569]" gridcol="12" gridrow="2" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FB1AFDCC1AF79731" box="[1158,1178,543,569]" gridcol="13" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB28FDCC1AAA9731" box="[1204,1223,543,569]" gridcol="14" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB7CFDCC1A999731" box="[1248,1268,543,569]" gridcol="15" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FA91FDCC1B4D9731" box="[1293,1312,543,569]" gridcol="16" gridrow="2" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FAA6FDCC1B1A9731" box="[1338,1399,543,569]" gridcol="17" gridrow="2" pageId="21" pageNumber="159">
.
<emphasis id="2ABCEA83FFC3FFFDFAFFFDF21B1D9731" box="[1379,1392,545,569]" italics="true" pageId="21" pageNumber="159">1</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FD821B1A9763" box="[191,1399,593,619]" gridrow="3" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FD821F729763" box="[191,287,593,619]" gridcol="0" gridrow="3" pageId="21" pageNumber="159">y-290G</th>
<td id="E5295DAFFFC30017FEA0FD821F239763" box="[316,334,593,619]" gridcol="1" gridrow="3" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FEFDFD821C199763" box="[353,628,593,619]" gridcol="2" gridrow="3" pageId="21" pageNumber="159">
. T
<emphasis id="2ABCEA83FFC3FFFDFE3FFD801C1F9763" bold="true" box="[419,626,595,619]" pageId="21" pageNumber="159">– – – – – – –</emphasis>
</td>
<td id="E5295DAFFFC30017FD1BFD821CB39763" box="[647,734,593,619]" gridcol="3" gridrow="3" pageId="21" pageNumber="159">T A A</td>
<td id="E5295DAFFFC30017FD6FFD821D6B9763" box="[755,774,593,619]" gridcol="4" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCBCFD821D599763" box="[800,820,593,619]" gridcol="5" gridrow="3" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCD1FD821D0D9763" box="[845,864,593,619]" gridcol="6" gridrow="3" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCE6FD821DE19763" box="[890,908,593,619]" gridcol="7" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC3AFD821DD79763" box="[934,954,593,619]" gridcol="8" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC4FFD821D8B9763" box="[979,998,593,619]" gridcol="9" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB9CFD821A7E9763" box="[1024,1043,593,619]" gridcol="10" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBB1FD821A2D9763" box="[1069,1088,593,619]" gridcol="11" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBC6FD821A009763" box="[1114,1133,593,619]" gridcol="12" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB1AFD821AF79763" box="[1158,1178,593,619]" gridcol="13" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB28FD821AAA9763" box="[1204,1223,593,619]" gridcol="14" gridrow="3" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FB7CFD821A999763" box="[1248,1268,593,619]" gridcol="15" gridrow="3" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FA91FD821B4D9763" box="[1293,1312,593,619]" gridcol="16" gridrow="3" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FAA6FD821B1A9763" box="[1338,1399,593,619]" gridcol="17" gridrow="3" pageId="21" pageNumber="159">
A
<emphasis id="2ABCEA83FFC3FFFDFAC0FD801B1A9763" box="[1372,1399,595,619]" italics="true" pageId="21" pageNumber="159">18</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FD571B1A9796" box="[191,1399,644,670]" gridrow="4" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FD571F729796" box="[191,287,644,670]" gridcol="0" gridrow="4" pageId="21" pageNumber="159">y-290A</th>
<td id="E5295DAFFFC30017FEA0FD571F239796" box="[316,334,644,670]" gridcol="1" gridrow="4" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FEFDFD571C199796" box="[353,628,644,670]" gridcol="2" gridrow="4" pageId="21" pageNumber="159">
. T
<emphasis id="2ABCEA83FFC3FFFDFE3FFD551C1F9796" bold="true" box="[419,626,646,670]" pageId="21" pageNumber="159">– – – – – – –</emphasis>
</td>
<td id="E5295DAFFFC30017FD1BFD571CB39796" box="[647,734,644,670]" gridcol="3" gridrow="4" pageId="21" pageNumber="159">T A A</td>
<td id="E5295DAFFFC30017FD6FFD571D6B9796" box="[755,774,644,670]" gridcol="4" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCBCFD571D599796" box="[800,820,644,670]" gridcol="5" gridrow="4" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCD1FD571D0D9796" box="[845,864,644,670]" gridcol="6" gridrow="4" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCE6FD571DE19796" box="[890,908,644,670]" gridcol="7" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC3AFD571DD79796" box="[934,954,644,670]" gridcol="8" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC4FFD571D8B9796" box="[979,998,644,670]" gridcol="9" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB9CFD571A7E9796" box="[1024,1043,644,670]" gridcol="10" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBB1FD571A2D9796" box="[1069,1088,644,670]" gridcol="11" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBC6FD571A009796" box="[1114,1133,644,670]" gridcol="12" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB1AFD571AF79796" box="[1158,1178,644,670]" gridcol="13" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB28FD571AAA9796" box="[1204,1223,644,670]" gridcol="14" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB7CFD571A999796" box="[1248,1268,644,670]" gridcol="15" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FA91FD571B4D9796" box="[1293,1312,644,670]" gridcol="16" gridrow="4" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FAA6FD571B1A9796" box="[1338,1399,644,670]" gridcol="17" gridrow="4" pageId="21" pageNumber="159">
A
<emphasis id="2ABCEA83FFC3FFFDFAFFFD551B1D9796" box="[1379,1392,646,670]" italics="true" pageId="21" pageNumber="159">2</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FD641B1A97D9" box="[191,1399,695,721]" gridrow="5" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FD641F7297D9" box="[191,287,695,721]" gridcol="0" gridrow="5" pageId="21" pageNumber="159">y-24G</th>
<td id="E5295DAFFFC30017FEA0FD641F2397D9" box="[316,334,695,721]" gridcol="1" gridrow="5" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FEFDFD641C1997D9" box="[353,628,695,721]" gridcol="2" gridrow="5" pageId="21" pageNumber="159">
G T
<emphasis id="2ABCEA83FFC3FFFDFE3FFD6B1C1F97D8" bold="true" box="[419,626,696,720]" pageId="21" pageNumber="159">– – – – – – –</emphasis>
</td>
<td id="E5295DAFFFC30017FD1BFD641CB397D9" box="[647,734,695,721]" gridcol="3" gridrow="5" pageId="21" pageNumber="159">T A A</td>
<td id="E5295DAFFFC30017FD6FFD641D6B97D9" box="[755,774,695,721]" gridcol="4" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCBCFD641D5997D9" box="[800,820,695,721]" gridcol="5" gridrow="5" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCD1FD641D0D97D9" box="[845,864,695,721]" gridcol="6" gridrow="5" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FCE6FD641DE197D9" box="[890,908,695,721]" gridcol="7" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC3AFD641DD797D9" box="[934,954,695,721]" gridcol="8" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FC4FFD641D8B97D9" box="[979,998,695,721]" gridcol="9" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB9CFD641A7E97D9" box="[1024,1043,695,721]" gridcol="10" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBB1FD641A2D97D9" box="[1069,1088,695,721]" gridcol="11" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FBC6FD641A0097D9" box="[1114,1133,695,721]" gridcol="12" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB1AFD641AF797D9" box="[1158,1178,695,721]" gridcol="13" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB28FD641AAA97D9" box="[1204,1223,695,721]" gridcol="14" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FB7CFD641A9997D9" box="[1248,1268,695,721]" gridcol="15" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FA91FD641B4D97D9" box="[1293,1312,695,721]" gridcol="16" gridrow="5" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FAA6FD641B1A97D9" box="[1338,1399,695,721]" gridcol="17" gridrow="5" pageId="21" pageNumber="159">
A
<emphasis id="2ABCEA83FFC3FFFDFAFFFD6B1B1D97D8" box="[1379,1392,696,720]" italics="true" pageId="21" pageNumber="159">1</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FD3A1B1A960B" box="[191,1399,745,771]" gridrow="6" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FD3A1F72960B" box="[191,287,745,771]" gridcol="0" gridrow="6" pageId="21" pageNumber="159">w-377G</th>
<td id="E5295DAFFFC30017FEA0FD3A1F23960B" box="[316,334,745,771]" gridcol="1" gridrow="6" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FEFDFD3A1C19960B" box="[353,628,745,771]" gridcol="2" gridrow="6" pageId="21" pageNumber="159">
..
<emphasis id="2ABCEA83FFC3FFFDFE3FFD381FDD960B" bold="true" box="[419,432,747,771]" pageId="21" pageNumber="159">–</emphasis>
.... C.
</td>
<td id="E5295DAFFFC30017FD1BFD3A1CB3960B" box="[647,734,745,771]" gridcol="3" gridrow="6" pageId="21" pageNumber="159">...</td>
<td id="E5295DAFFFC30017FD6FFD3A1D6B960B" box="[755,774,745,771]" gridcol="4" gridrow="6" pageId="21" pageNumber="159">C</td>
<td id="E5295DAFFFC30017FCBCFD3A1D59960B" box="[800,820,745,771]" gridcol="5" gridrow="6" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCD1FD3A1D0D960B" box="[845,864,745,771]" gridcol="6" gridrow="6" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCE6FD3A1DE1960B" box="[890,908,745,771]" gridcol="7" gridrow="6" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FC3AFD3A1DD7960B" box="[934,954,745,771]" gridcol="8" gridrow="6" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FC4FFD3A1D8B960B" box="[979,998,745,771]" gridcol="9" gridrow="6" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFC4AFD381D8E960B" bold="true" box="[982,995,747,771]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FB9CFD3A1A7E960B" box="[1024,1043,745,771]" gridcol="10" gridrow="6" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFB9FFD381A7D960B" bold="true" box="[1027,1040,747,771]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FBB1FD3A1A2D960B" box="[1069,1088,745,771]" gridcol="11" gridrow="6" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFBACFD381A50960B" bold="true" box="[1072,1085,747,771]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FBC6FD3A1A00960B" box="[1114,1133,745,771]" gridcol="12" gridrow="6" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFBC0FD381A04960B" bold="true" box="[1116,1129,747,771]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FB1AFD3A1AF7960B" box="[1158,1178,745,771]" gridcol="13" gridrow="6" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FB28FD3A1AAA960B" box="[1204,1223,745,771]" gridcol="14" gridrow="6" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FB7CFD3A1A99960B" box="[1248,1268,745,771]" gridcol="15" gridrow="6" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FA91FD3A1B4D960B" box="[1293,1312,745,771]" gridcol="16" gridrow="6" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FAA6FD3A1B1A960B" box="[1338,1399,745,771]" gridcol="17" gridrow="6" pageId="21" pageNumber="159">
.
<emphasis id="2ABCEA83FFC3FFFDFAC1FD381B1B960B" box="[1373,1398,747,771]" italics="true" pageId="21" pageNumber="159">11</emphasis>
</td>
</tr>
<tr id="A6F834D3FFC30017FF23FCCF1B1A963E" box="[191,1399,796,822]" gridrow="7" pageId="21" pageNumber="159">
<th id="E5295DAFFFC30017FF23FCCF1F72963E" box="[191,287,796,822]" gridcol="0" gridrow="7" pageId="21" pageNumber="159">w-377T</th>
<td id="E5295DAFFFC30017FEA0FCCF1F23963E" box="[316,334,796,822]" gridcol="1" gridrow="7" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FEFDFCCF1C19963E" box="[353,628,796,822]" gridcol="2" gridrow="7" pageId="21" pageNumber="159">
..
<emphasis id="2ABCEA83FFC3FFFDFE3FFCCD1FDD963E" bold="true" box="[419,432,798,822]" pageId="21" pageNumber="159">–</emphasis>
.... C.
</td>
<td id="E5295DAFFFC30017FD1BFCCF1CB3963E" box="[647,734,796,822]" gridcol="3" gridrow="7" pageId="21" pageNumber="159">...</td>
<td id="E5295DAFFFC30017FD6FFCCF1D6B963E" box="[755,774,796,822]" gridcol="4" gridrow="7" pageId="21" pageNumber="159">C</td>
<td id="E5295DAFFFC30017FCBCFCCF1D59963E" box="[800,820,796,822]" gridcol="5" gridrow="7" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCD1FCCF1D0D963E" box="[845,864,796,822]" gridcol="6" gridrow="7" pageId="21" pageNumber="159">.</td>
<td id="E5295DAFFFC30017FCE6FCCF1DE1963E" box="[890,908,796,822]" gridcol="7" gridrow="7" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FC3AFCCF1DD7963E" box="[934,954,796,822]" gridcol="8" gridrow="7" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FC4FFCCF1D8B963E" box="[979,998,796,822]" gridcol="9" gridrow="7" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFC4AFCCD1D8E963E" bold="true" box="[982,995,798,822]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FB9CFCCF1A7E963E" box="[1024,1043,796,822]" gridcol="10" gridrow="7" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFB9FFCCD1A7D963E" bold="true" box="[1027,1040,798,822]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FBB1FCCF1A2D963E" box="[1069,1088,796,822]" gridcol="11" gridrow="7" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFBACFCCD1A50963E" bold="true" box="[1072,1085,798,822]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FBC6FCCF1A00963E" box="[1114,1133,796,822]" gridcol="12" gridrow="7" pageId="21" pageNumber="159">
<emphasis id="2ABCEA83FFC3FFFDFBC0FCCD1A04963E" bold="true" box="[1116,1129,798,822]" pageId="21" pageNumber="159">–</emphasis>
</td>
<td id="E5295DAFFFC30017FB1AFCCF1AF7963E" box="[1158,1178,796,822]" gridcol="13" gridrow="7" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FB28FCCF1AAA963E" box="[1204,1223,796,822]" gridcol="14" gridrow="7" pageId="21" pageNumber="159">A</td>
<td id="E5295DAFFFC30017FB7CFCCF1A99963E" box="[1248,1268,796,822]" gridcol="15" gridrow="7" pageId="21" pageNumber="159">G</td>
<td id="E5295DAFFFC30017FA91FCCF1B4D963E" box="[1293,1312,796,822]" gridcol="16" gridrow="7" pageId="21" pageNumber="159">T</td>
<td id="E5295DAFFFC30017FAA6FCCF1B1A963E" box="[1338,1399,796,822]" gridcol="17" gridrow="7" pageId="21" pageNumber="159">. 1</td>
</tr>
</table>
</paragraph>
<paragraph id="18773691FFC3FFFDFF21FC551A6B96CB" blockId="21.[189,1399,902,964]" pageId="21" pageNumber="159">Älv, Ljusnan and Dalälven) several variants are present. Only one variant is found in Enningdalsälven and Ljungan, where two and three animals, respectively, were assessed.</paragraph>
<paragraph id="18773691FFC3FFFDFF21FC391FD39199" blockId="21.[189,1400,1002,1169]" pageId="21" pageNumber="159">
Variants y-290G and w-377G are distributed over the entire study area, whereas variant z-242T is restricted to the middle-west catchment areas (see
<figureCitation id="80F32A14FFC3FFFDFCD9FBDD1DE39120" box="[837,910,1038,1064]" captionStart="Fig" captionStartId="24.[189,232,1817,1843]" captionTargetBox="[193,1395,269,1772]" captionTargetId="figure-12@24.[189,1398,267,1772]" captionTargetPageId="24" captionText="Fig. 8. Presence of i56 sequence varieties in Spongilla lacustris (Linnaeus, 1759) at different collecting sites. Black lines on the map represent watersheds between the catchment areas and cross hairs mark the actual sampling site. Seven sequence varieties were found, designated z-242T, z-242G, y-290G, y-290A, y-24G, w-377G and w-377T. Pie charts indicate fraction of the varieties at a site, and circle area corresponds to number of specimens sequenced from the site." figureDoi="http://doi.org/10.5281/zenodo.6839839" httpUri="https://zenodo.org/record/6839839/files/figure.png" pageId="21" pageNumber="159">Fig. 8</figureCitation>
). Interestingly, only these three variants (y-290G, w-377G and z-242T) were found in the west part of
<collectingCountry id="60DF7601FFC3FFFDFC3BFBE21A659143" box="[935,1032,1073,1099]" name="Sweden" pageId="21" pageNumber="159">Sweden</collectingCountry>
. Variants y-290A, w-377T and z-242G are found in the same catchment areas as their closest allelic counterpart (y-290G, w-377G and z-242T, respectively).
</paragraph>
<paragraph id="18773691FFC3FFFDFF21FB6A1C9391FE" blockId="21.[187,1398,1209,1271]" pageId="21" pageNumber="159">Results of AMOVA (analysis of molecular variance) show more variation within than among populations (59.8% and 40.2% of the variation, respectively).</paragraph>
</subSubSection>
</treatment>
</document>
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