159 lines
34 KiB
XML
159 lines
34 KiB
XML
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<mods:title id="04DA4D7912E23E87E9444678FAC85A1D">Tracking the trends of karyotype differentiation in the phylogenetic context of Gint, a scorpion genus endemic to the Horn of Africa (Scorpiones: Buthidae)</mods:title>
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<mods:namePart id="35C224A92C88597CC1B4BDB7C1CF0988">Just, Pavel</mods:namePart>
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<mods:namePart id="D058869AF2D2D2FF7435A0D3412C7ED4">Kovařík, František</mods:namePart>
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<mods:namePart id="43791999D73AE82D316CCEABE2D769C6">Štundlová, Jana</mods:namePart>
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<treatment id="03AACF58FFA81F20FEC415FDFD1593C0" ID-DOI="http://doi.org/10.5281/zenodo.7194670" ID-GBIF-Taxon="202609289" ID-Zenodo-Dep="7194670" LSID="urn:lsid:plazi:treatment:03AACF58FFA81F20FEC415FDFD1593C0" httpUri="http://treatment.plazi.org/id/03AACF58FFA81F20FEC415FDFD1593C0" lastPageId="8" lastPageNumber="893" pageId="7" pageNumber="892">
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<subSubSection id="C3192DC5FFA81F2FFEC415FDFDC096E0" box="[364,577,1762,1785]" pageId="7" pageNumber="892" type="nomenclature">
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<paragraph id="8BBC7E4EFFA81F2FFEC415FDFDC096E0" blockId="7.[364,577,1762,1785]" box="[364,577,1762,1785]" pageId="7" pageNumber="892">
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<heading id="D0F4C922FFA81F2FFEC415FDFDC096E0" box="[364,577,1762,1785]" centered="true" fontSize="9" level="2" pageId="7" pageNumber="892" reason="2">
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<taxonomicName id="4C0305CDFFA81F2FFEC415FDFDC096E0" ID-CoL="3G3HX" authorityName="Kovarik, Lowe, Just, Awale, Elmi & Stahlavsky" authorityYear="2018" box="[364,577,1762,1785]" class="Arachnida" family="Buthidae" genus="Gint" kingdom="Animalia" order="Scorpiones" pageId="7" pageNumber="892" phylum="Arthropoda" rank="species" species="amoudensis">
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<emphasis id="B977A25CFFA81F2FFEC415FDFDC096E0" box="[364,577,1762,1785]" italics="true" pageId="7" pageNumber="892">
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<emphasis id="B977A25CFFA81F2FFEC415FDFE2696EE" box="[364,423,1762,1785]" italics="true" pageId="7" pageNumber="892">GINT</emphasis>
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AMOUDENSIS
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</emphasis>
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</taxonomicName>
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</heading>
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</paragraph>
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<subSubSection id="C3192DC5FFA81F2FFF0B1415FC2D954A" pageId="7" pageNumber="892" type="discussion">
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<paragraph id="8BBC7E4EFFA81F2FFF0B1415FC2D954A" blockId="7.[163,779,1802,1916]" lastBlockId="7.[824,1444,1250,1916]" pageId="7" pageNumber="892">
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T h e m o s t s t r i k i n g i n t r a s p e c i f i c v a r i a b i l i t y i s discovered in
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<taxonomicName id="4C0305CDFFA81F2FFEFA1436FD879724" box="[338,518,1832,1854]" class="Arachnida" family="Buthidae" genus="Gint" kingdom="Animalia" order="Scorpiones" pageId="7" pageNumber="892" phylum="Arthropoda" rank="species" species="amoudensis">
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<emphasis id="B977A25CFFA81F2FFEFA1436FD879724" box="[338,518,1832,1854]" italics="true" pageId="7" pageNumber="892">G. amoudensis</emphasis>
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</taxonomicName>
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, which exhibits four cytotypes differing in the diploid number, multivalent associations and in the number and position of 18S rDNA sites. The cytotypes II, III and IV occur sympatrically, while the cytotype I (S1325) is found in a remote locality Borama
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<emphasis id="B977A25CFFA81F2FFBCA163FFBEC952C" box="[1122,1133,1312,1333]" italics="true" pageId="7" pageNumber="892">c</emphasis>
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.
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<quantity id="4CFBD3ABFFA81F2FFBD21600FB4E952C" box="[1146,1231,1311,1333]" metricMagnitude="5" metricUnit="m" metricValue="1.5" pageId="7" pageNumber="892" unit="km" value="150.0">150 km</quantity>
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far from the other cytotypes.
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<subSubSection id="C3192DC5FFA81F20FCFB1642FD1593C0" lastPageId="8" lastPageNumber="893" pageId="7" pageNumber="892" type="description">
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<paragraph id="8BBC7E4EFFA81F2FFCFB1642FB4E96BD" blockId="7.[824,1444,1250,1916]" pageId="7" pageNumber="892">
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<emphasis id="B977A25CFFA81F2FFCFB1642FC42956B" box="[851,963,1373,1394]" italics="true" pageId="7" pageNumber="892">Cytotype I</emphasis>
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(1Ƌ; S1325) exhibits 2
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<emphasis id="B977A25CFFA81F2FFB151642FB4D956B" box="[1213,1228,1373,1394]" italics="true" pageId="7" pageNumber="892">n</emphasis>
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= 36 chromosomes, which decrease in length from 4.49% to 1.63% of DSL (Supporting Information,
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<figureCitation id="133862CBFFA81F2FFBD11685FB6595B6" box="[1145,1252,1434,1456]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="7" pageNumber="892">Fig. S2C</figureCitation>
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; Table S2). The post-pachytene nuclei show 16 bivalents and one quadrivalent (chromosomes 1, 2, 9 and 15) (
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<figureCitation id="133862CBFFA81F2FFAEF16C8FA1C95F5" box="[1351,1437,1495,1517]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="7" pageNumber="892">Fig. 3A</figureCitation>
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; Supporting Information,
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<figureCitation id="133862CBFFA81F2FFBCA16E9FB479612" box="[1122,1222,1526,1548]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="7" pageNumber="892">Fig. S2C</figureCitation>
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). Three 18S rDNA sites are detected (
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<figureCitation id="133862CBFFA81F2FFBB5150BFBF39633" box="[1053,1138,1556,1578]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="7" pageNumber="892">Fig. 3B</figureCitation>
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), two of them are located in the subterminal region of the chromosomes 1 and 2 involved in the quadrivalent and one in the terminal position of chromosome
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<quantity id="4CFBD3ABFFA81F2FFB1B156EFB5F969F" box="[1203,1246,1648,1670]" metricMagnitude="-2" metricUnit="m" metricValue="5.08" pageId="7" pageNumber="892" unit="in" value="2.0">2 in</quantity>
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the quadrivalent (Supporting Information,
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<figureCitation id="133862CBFFA81F2FFBF71590FB4196BD" box="[1119,1216,1679,1701]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="7" pageNumber="892">Fig. S2C</figureCitation>
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).
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</paragraph>
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<paragraph id="8BBC7E4EFFA81F20FCFB15B1FD9E916D" blockId="7.[824,1444,1250,1916]" lastBlockId="8.[145,762,197,986]" lastPageId="8" lastPageNumber="893" pageId="7" pageNumber="892">
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<emphasis id="B977A25CFFA81F2FFCFB15B1FC5296DA" box="[851,979,1710,1731]" italics="true" pageId="7" pageNumber="892">Cytotype II</emphasis>
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(1 Ƌ; S1291) has 2
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<emphasis id="B977A25CFFA81F2FFB0715B1FB3F96DA" box="[1199,1214,1710,1731]" italics="true" pageId="7" pageNumber="892">n</emphasis>
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= 35 chromosomes, which decrease in length from 5.01% to 1.45% of DSL (Supporting Information,
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<figureCitation id="133862CBFFA81F2FFBD015F4FB629719" box="[1144,1251,1771,1793]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="7" pageNumber="892">Fig. S2D</figureCitation>
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; Table S2). The post-pachytene spermatocytes exhibit 14 bivalents, one trivalent (chromosomes 4, 24 and 35) and one quadrivalent (chromosomes 1, 5, 8 and 23) (
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<figureCitation id="133862CBFFA81F2FFAEF1458FA1C9745" box="[1351,1437,1863,1885]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="7" pageNumber="892">Fig. 3C</figureCitation>
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; Supporting Information,
|
||
<figureCitation id="133862CBFFA81F2FFBC91479FB479762" box="[1121,1222,1894,1916]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="7" pageNumber="892">Fig. S2D</figureCitation>
|
||
). Three 18S rDNA loci are present (
|
||
<figureCitation id="133862CBFFA71F20FEF313DAFE3390C3" box="[347,434,197,219]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3D</figureCitation>
|
||
), one pair is located on the chromosomes 1 (subterminal site) and 5 (terminal site) involved in the quadrivalent, and one single heterozygous locus is situated in the interstitial region of the third largest bivalent (chromosome 9;
|
||
<figureCitation id="133862CBFFA71F20FD35125FFD73914C" box="[669,754,320,342]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3D</figureCitation>
|
||
; Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FE051241FD8E916D" box="[429,527,350,372]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2D</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BBC7E4EFFA71F20FF011262FD9C9291" blockId="8.[145,762,197,986]" pageId="8" pageNumber="893">
|
||
<emphasis id="B977A25CFFA71F20FF011262FEB0918A" box="[169,305,381,403]" italics="true" pageId="8" pageNumber="893">Cytotype III</emphasis>
|
||
(1 Ƌ; S1293) has 2
|
||
<emphasis id="B977A25CFFA71F20FDA11261FD99918A" box="[521,536,382,403]" italics="true" pageId="8" pageNumber="893">n</emphasis>
|
||
= 36 chromosomes, which decrease in length from 4.71% to 1.52% of DSL (Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FDA112A5FDF291C9" box="[521,627,442,464]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2E</figureCitation>
|
||
; Table S2). The post-pachytene cells show 15 bivalents and one hexavalent (chromosomes 1, 2, 5, 6, 11 and 24) (
|
||
<figureCitation id="133862CBFFA71F20FF311109FF6A9235" box="[153,235,534,556]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3E</figureCitation>
|
||
; Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FDB31109FDFC9235" box="[539,637,534,556]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2E</figureCitation>
|
||
). One pair of 18S rDNA sites is located in the subterminal region of the chromosomes 1 and 2 of the hexavalent (
|
||
<figureCitation id="133862CBFFA71F20FD0C114BFD739270" box="[676,754,596,618]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3F</figureCitation>
|
||
; Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FE05116DFD8F9291" box="[429,526,626,648]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BBC7E4EFFA71F20FF01118EFD1593C0" blockId="8.[145,762,197,986]" pageId="8" pageNumber="893">
|
||
<emphasis id="B977A25CFFA71F20FF01118EFEC892BE" box="[169,329,657,679]" italics="true" pageId="8" pageNumber="893">Cytotype IV</emphasis>
|
||
(1 Ƌ; S1292) exhibits 2
|
||
<emphasis id="B977A25CFFA71F20FD36118DFD2C92BE" box="[670,685,658,679]" italics="true" pageId="8" pageNumber="893">n</emphasis>
|
||
= 35 chromosomes, which decrease in length from 4.66% to 1.54% of DSL (Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FF3911F2FE80931B" box="[145,257,749,771]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2F</figureCitation>
|
||
; Table S2). The post-pachytene nuclei show a configuration of 13 bivalents, one trivalent (chromosomes 7, 26 and 35) and one hexavalent (chromosomes 1, 4, 5, 6, 10 and 23) (
|
||
<figureCitation id="133862CBFFA71F20FD271056FD719347" box="[655,752,841,863]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3G</figureCitation>
|
||
; Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FE171077FDA59364" box="[447,548,872,894]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2F</figureCitation>
|
||
). One pair of 18S rDNA sites is located in the subterminal region of the chromosomes 1 and 4 involved in the hexavalent (
|
||
<figureCitation id="133862CBFFA71F20FF3110DBFF6E93C0" box="[153,239,964,986]" captionStart="Figure 3" captionStartId="6.[145,223,1192,1214]" captionTargetBox="[153,1417,198,1149]" captionTargetId="figure-348@6.[150,1420,195,1152]" captionTargetPageId="6" captionText="Figure 3. Post-pachytene cells of Gint amoudensis and G. gubanensis after Giemsa staining (A, C, E, G, I) and FISH with 18S rDNA (red signals) (B, D, F, H, J). A, B, G. amoudensis cytotype I (2n = 36 – 16II + IV). C, D, G. amoudensis cytotype II (2n = 35 – 14II + III + IV). E, F, G. amoudensis cytotype III (2n = 36 – 15II + VI). G, H, G. amoudensis cytotype IV (2n = 35 – 13II + III + VI). I, J, G.gubanensis (2n = 45 – 21II + III). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184502" httpUri="https://zenodo.org/record/7184502/files/figure.png" pageId="8" pageNumber="893">Fig. 3H</figureCitation>
|
||
; Supporting Information,
|
||
<figureCitation id="133862CBFFA71F20FD8B10DBFD0593C0" box="[547,644,964,986]" captionStart="Figure 2" captionStartId="5.[163,241,1512,1534]" captionTargetBox="[171,1436,200,1467]" captionTargetId="figure-117@5.[166,1440,195,1472]" captionTargetPageId="5" captionText="Figure 2. Post-pachytene cells of Gint species after Giemsa staining (A, C, E, G, I, K, M, O) and FISH with 18S rDNA (red signals) (B, D, F, H, J,L, N, P).A, B,G. banfasae cytotype I(2n=18 –7II + IV).C, D, G. banfasae cytotype II (2n= 18 – 6II +VI). E, F, G. banfasae cytotype III (2n = 19 – 5II + III + VI). G, H, G. dabakalo cytotype I (2n = 23 – 9II + V). I, J,G. dabakalo cytotype II (2n = 24 – 8II + III + V). K, L, G. dabakalo cytotype III (2n = 27 – 8II + 2III + V). M, N, G. gaitako (2n = 30 – 13II + IV). O, P, G. maidensis (2n = 34). Abbreviations: II, bivalent; III, trivalent; IV, quadrivalent; V, pentavalent; VI, hexavalent. Arrowheads indicate the position of 18S rDNA. Scale bar = 10 µm." figureDoi="http://doi.org/10.5281/zenodo.7184498" httpUri="https://zenodo.org/record/7184498/files/figure.png" pageId="8" pageNumber="893">Fig. S2F</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |