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<document id="C9BD7E247BEB348D9E36B1D497598A71" ID-DOI="10.5281/zenodo.10396362" ID-ISSN="1942-1354" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1707168658757" checkinUser="felipe" docAuthor="Zhang, Jing, Cong, Qian &amp; Grishin, Nick V." docDate="2023" docId="03810139FFC2BB4DC0CAFE8AE723B449" docLanguage="en" docName="InsectaMundi.2023.1026.1-115.pdf" docOrigin="Insecta Mundi 2023 (26)" docSource="http://dx.doi.org/10.5281/zenodo.10396362" docStyle="DocumentStyle:F9976E9B7F8A094D890004515A7A0592.3:InsectaMundi.2020-.journal_article" docStyleId="F9976E9B7F8A094D890004515A7A0592" docStyleName="InsectaMundi.2020-.journal_article" docStyleVersion="3" docTitle="Myrinia orieca Zhang &amp; Cong &amp; Grishin 2023, new species" docType="treatment" docVersion="1" lastPageNumber="28" masterDocId="FFB87941FFDFBB50C00AFF56E355B262" masterDocTitle="Supplementary Materials and Appendix" masterLastPageNumber="115" masterPageNumber="1" pageNumber="28" updateTime="1707169288913" updateUser="GgImagineBatch" zenodo-license-document="CC-BY-NC-3.0">
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<mods:title id="1933754CECA3EB74012BBFA9CB70BE87">Supplementary Materials and Appendix</mods:title>
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<mods:namePart id="9CDBD86027CAEF22B2BA0502258B519B">Zhang, Jing</mods:namePart>
<mods:affiliation id="FBABEE773837F6E2B1B591BF9F6C1FCE">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="7B9CAD44B917CD2FB2555458E71C1D5D">Cong, Qian</mods:namePart>
<mods:affiliation id="8AB296643688EBA84C870D448C2B8BCF">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="82D9962234B3FD1EA10EBF8A1E260C37">Grishin, Nick V.</mods:namePart>
<mods:affiliation id="9F626C7975609066821EC05992A7E5CE">Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA</mods:affiliation>
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<mods:title id="32A3169991020B64FD3175D87BD0E9CC">Insecta Mundi</mods:title>
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<mods:date id="07F349DDD91591B45D4123F7B4B29338">2023</mods:date>
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<emphasis id="B95C6C3DFFC2BB4DC0CAFE8AE1DDB39A" bold="true" box="[192,648,475,504]" pageId="29" pageNumber="28">
<taxonomicName id="4C28CBACFFC2BB4DC0CAFE8AE2B2B39A" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[192,487,475,504]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="29" pageNumber="28" phylum="Arthropoda" rank="species" species="orieca" status="sp. nov.">
<emphasis id="B95C6C3DFFC2BB4DC0CAFE8AE22EB39A" bold="true" box="[192,379,476,504]" italics="true" pageId="29" pageNumber="28">Myrinia orieca</emphasis>
Grishin
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,
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https://zoobank.org/
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<paragraph id="8B97B02FFFC2BB4DC0CAFD77E157B059" blockId="29.[192,933,475,572]" box="[192,514,545,572]" pageId="29" pageNumber="28">
(
<figureCitation id="1313ACAAFFC2BB4DC0C2FD77E252B059" box="[200,263,545,571]" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="29" pageNumber="28">Fig. 2</figureCitation>
part, 6162, 274276)
</paragraph>
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<subSubSection id="C332E3A4FFC2BB4DC0CAFD1DE7D8B611" pageId="29" pageNumber="28" type="diagnosis">
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<emphasis id="B95C6C3DFFC2BB4DC0CAFD1DE2BDB007" bold="true" box="[192,488,587,613]" pageId="29" pageNumber="28">Definition and diagnosis.</emphasis>
Phylogenetic analysis reveals that specimens from eastern
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identified as
<taxonomicName id="4C28CBACFFC2BB4DC563FD1AE14FB0E5" authority="(Moschler, 1877)" baseAuthorityName="Moschler" baseAuthorityYear="1877" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="29" pageNumber="28" phylum="Arthropoda" rank="species" species="binoculus">
<emphasis id="B95C6C3DFFC2BB4DC563FD1AE20CB0E5" italics="true" pageId="29" pageNumber="28">Myrinia binoculus</emphasis>
(Möschler, 1877)
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(type locality in
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,
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sequenced as NVG-15033C11) are genetically differentiated from it in both nuclear and mitochondrial genomes (
<figureCitation id="1313ACAAFFC2BB4DC3AEFDD8E0B0B0CA" box="[932,997,654,680]" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="29" pageNumber="28">Fig. 2</figureCitation>
): e.g., their COI barcodes differ by 1.8% (12 bp), and therefore represent a new species. This new species keys to
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<emphasis id="B95C6C3DFFC2BB4DC3FAFDE7E72AB0A8" box="[1008,1151,688,714]" italics="true" pageId="29" pageNumber="28">M. binoculus</emphasis>
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((E.24.1) in
<bibRefCitation id="EFB9CDDEFFC2BB4DC505FDE7E6F4B0A9" author="Evans WH" box="[1295,1441,688,715]" pageId="29" pageNumber="28" pagination="26 - 53" refId="ref77110" refString="Evans WH. 1953. A catalogue of the American Hesperiidae indicating the classification and nomenclature adopted in the British Museum (Natural History). Part III. Pyrginae. Section 2. The Trustees of the British Museum (Natural History); London. v + 246 p., pl. 26 - 53." type="book chapter" year="1953">Evans (1953)</bibRefCitation>
and differs from it by more elongated eyespot on the dorsal forewing, wider dark-brown forewing margins, better defined and more contrasting from the ground color bands (discal, postdiscal, and submarginal) on the ventral hindwing, and a smaller dark ventral hindwing tornal spot (
<figureCitation id="1313ACAAFFC2BB4DC397FC43E74FB14D" box="[925,1050,789,815]" captionStart="Figures 4962" captionStartId="97.[192,271,1841,1865]" captionTargetBox="[199,1416,304,1773]" captionTargetId="figure-56@97.[194,1442,296,1781]" captionTargetPageId="97" captionText="Figures 4962. Holotypes (unless indicated), data in text. 4952) Pseudodrephalys tinas sp. n.: 5152) paratype ♀ NVG- 19039F11. 5354) Pseudodrephalys argus sp. n. 5556) Achlyodes calvus sp. n. 5758) Spioniades artemis sp. n. 5960) Spioniades artemidoides sp. n. 6162) Myrinia orieca sp. n." pageId="29" pageNumber="28">Fig. 6162</figureCitation>
). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly2178.55.2:C48T, aly2178.55.2:C63T, aly1084.13.12:C93T, aly276558.43.4:A408G, aly4645.9.3:T103C, and COI barcode: A43G, A79G, T97C, A166G, A184G, A586G.
</paragraph>
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<emphasis id="B95C6C3DFFC2BB4DC0CAFCF3E11BB1DD" bold="true" box="[192,590,932,959]" pageId="29" pageNumber="28">
Barcode sequence of the
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.
</emphasis>
Sample NVG-17105F03, GenBank OR837649, 658 base pairs: TACTTTATACTTTATTTTTGGAATTTGAGCAGGAATAGTAGGGACATCCTTAAGTTTATTAATTCGTACTGAATTAGGGAATCCAGGATCATTAATC GGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATGCCAATTATAATTGGAGGGTTTGGAAATT GACTTGTTCCATTAATATTAGGTGCCCCAGATATAGCTTTTCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCATCATTAATATTATTAAT TTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTACCCTCCTTTATCTGCTAATATTGCTCATCAAGGATCCTCAGTAGATTTA GCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGTATTAATAATCTTTCAT TCGATCAAATACCTTTATTTGTATGAGCAGTAGGAATTACAGCTTTATTATTACTATTATCTTTACCTGTATTAGCAGGAGCAATTACTATACTTTT AACGGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGTGGGGGAGATCCTATTTTATATCAACACTTATTT
</paragraph>
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<emphasis id="B95C6C3DFFC2BB4DC0CAFBD0E239B6FD" bold="true" box="[192,364,1157,1184]" pageId="29" pageNumber="28">Type material.</emphasis>
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deposited in the
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, Washington,
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), illustrated in
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, bears the following four rectangular printed labels, three white: [
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, | Tiputini Biodiversity Station, | Lower Río Tiputini, |
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|
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&amp;
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], [DNA sample ID: | NVG-17105F03 | c/o
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.
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], [USNMENT | {
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} | 00894950], and one red [
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|
<taxonomicName id="4C28CBACFFC2BB4DC3D7FA5AE762B744" authorityName="Evans" authorityYear="1953" box="[989,1079,1292,1318]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="29" pageNumber="28" phylum="Arthropoda" rank="genus">Myrinia</taxonomicName>
|
<taxonomicName id="4C28CBACFFC2BB4DC441FA5AE7B9B744" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[1099,1260,1292,1318]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="29" pageNumber="28" phylum="Arthropoda" rank="species" species="orieca">orieca Grishin</taxonomicName>
]
</materialsCitation>
.
<emphasis id="B95C6C3DFFC2BB4DC502FA5BE62DB745" bold="true" box="[1288,1400,1293,1319]" pageId="29" pageNumber="28">
<typeStatus id="54930E8DFFC2BB4DC502FA5BE627B745" box="[1288,1394,1293,1319]" pageId="29" pageNumber="28" type="paratype">Paratype</typeStatus>
:
</emphasis>
<specimenCount id="9D2E7BA6FFC2BB4DC574FA5BE6F5B748" box="[1406,1440,1293,1322]" count="1" pageId="29" pageNumber="28" type="male">1♂</specimenCount>
NVG-14112H01
<collectingCountry id="F33FF0BFFFC2BB4DC18BFA78E2B6B72A" box="[385,483,1326,1352]" name="Ecuador" pageId="29" pageNumber="28">Ecuador</collectingCountry>
:
<collectingRegion id="49EC7ECDFFC2BB4DC1E5FA79E178B72B" box="[495,557,1327,1353]" country="Ecuador" name="Napo" pageId="29" pageNumber="28">Napo</collectingRegion>
, Yasuní National Park,
<date id="FF9696EFFFC2BB4DC333FA78E742B72A" box="[825,1047,1326,1352]" pageId="29" pageNumber="28" value="2003-09-20" valueMax="2003-10-04" valueMin="2003-09-20">20-Sep4-Oct-2003</date>
, C. Bordelon and E. Knudson, the Texas
<taxonomicName id="4C28CBACFFC2BB4DC10DFA06E2B4B708" authority="Survey" authorityName="Survey" box="[263,481,1360,1386]" class="Insecta" kingdom="Animalia" order="Lepidoptera" pageId="29" pageNumber="28" phylum="Arthropoda" rank="order">Lepidoptera Survey</taxonomicName>
collection [
<collectionCode id="ED3928EAFFC2BB4DC268FA06E1EFB708" box="[610,698,1360,1386]" pageId="29" pageNumber="28">MGCL</collectionCode>
].
</paragraph>
<paragraph id="8B97B02FFFC2BB4DC0CAFA2CE29BB7D7" blockId="29.[192,1440,1402,1462]" pageId="29" pageNumber="28">
<emphasis id="B95C6C3DFFC2BB4DC0CAFA2CE208B7F6" bold="true" box="[192,349,1402,1428]" pageId="29" pageNumber="28">
<typeStatus id="54930E8DFFC2BB4DC0CAFA2CE3ACB7F6" box="[192,249,1402,1428]" pageId="29" pageNumber="28">Type</typeStatus>
locality.
</emphasis>
<collectingCountry id="F33FF0BFFFC2BB4DC16FFA2CE292B7F6" box="[357,455,1402,1428]" name="Ecuador" pageId="29" pageNumber="28">Ecuador</collectingCountry>
:
<collectingRegion id="49EC7ECDFFC2BB4DC1D9FA2CE1F5B7F6" box="[467,672,1402,1428]" country="Ecuador" name="Orellana" pageId="29" pageNumber="28">Orellana Province</collectingRegion>
, Lower Río Tiputini, Tiputini Biodiversity Station, elevation
<quantity id="4CD01DCAFFC2BB4DC558FA2CE6C9B7F6" box="[1362,1436,1402,1428]" metricMagnitude="2" metricUnit="m" metricValue="2.0" pageId="29" pageNumber="28" unit="m" value="200.0">200 m</quantity>
,
<collectionCode id="ED3928EAFFC2BB4DC0C8FACAE3A6B7D4" box="[194,243,1436,1462]" pageId="29" pageNumber="28">GPS</collectionCode>
<geoCoordinate id="EE1CD6E8FFC2BB4DC0F3FACDE203B7D7" box="[249,342,1435,1461]" degrees="0.6319" direction="south" orientation="latitude" pageId="29" pageNumber="28" precision="5" value="-0.6319">0.6319</geoCoordinate>
,
<geoCoordinate id="EE1CD6E8FFC2BB4DC16AFACDE29FB7D7" box="[352,458,1435,1461]" degrees="76.1442" direction="west" orientation="longitude" pageId="29" pageNumber="28" precision="5" value="-76.1442">76.1442</geoCoordinate>
.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC2BB4DC0CAFA93E237B463" pageId="29" pageNumber="28" type="etymology">
<paragraph id="8B97B02FFFC2BB4DC0CAFA93E237B463" blockId="29.[192,1440,1477,1537]" pageId="29" pageNumber="28">
<emphasis id="B95C6C3DFFC2BB4DC0CAFA93E211B7BD" bold="true" box="[192,324,1477,1503]" pageId="29" pageNumber="28">Etymology.</emphasis>
This species comes from the
<emphasis id="B95C6C3DFFC2BB4DC28CFA90E1F9B782" box="[646,684,1478,1504]" italics="true" pageId="29" pageNumber="28">Ori</emphasis>
[ente (eastern region) in]
<emphasis id="B95C6C3DFFC2BB4DC3C2FA90E0B7B7BD" box="[968,994,1478,1503]" italics="true" pageId="29" pageNumber="28">Ec</emphasis>
[u]
<emphasis id="B95C6C3DFFC2BB4DC40FFA90E746B7BD" box="[1029,1043,1478,1503]" italics="true" pageId="29" pageNumber="28">a</emphasis>
[dor]. The name is a Latinized feminine adjective.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC2BB4DC0CAF947E723B449" box="[192,1142,1553,1579]" pageId="29" pageNumber="28" type="distribution">
<paragraph id="8B97B02FFFC2BB4DC0CAF947E723B449" blockId="29.[192,1142,1553,1579]" box="[192,1142,1553,1579]" pageId="29" pageNumber="28">
<emphasis id="B95C6C3DFFC2BB4DC0CAF947E20EB449" bold="true" box="[192,347,1553,1579]" pageId="29" pageNumber="28">Distribution.</emphasis>
Currently known only from the Amazonian region in eastern
<collectingCountry id="F33FF0BFFFC2BB4DC418F947E727B449" box="[1042,1138,1553,1579]" name="Ecuador" pageId="29" pageNumber="28">Ecuador</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>