132 lines
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132 lines
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<document id="125F3803E16502FA200596D282FA0B6F" ID-DOI="10.1016/j.phytochem.2018.07.009" ID-ISSN="1873-3700" ID-Zenodo-Dep="10484235" IM.bibliography_approvedBy="juliana" IM.illustrations_approvedBy="juliana" IM.materialsCitations_approvedBy="juliana" IM.metadata_approvedBy="juliana" IM.tables_approvedBy="juliana" IM.taxonomicNames_approvedBy="juliana" IM.treatments_approvedBy="juliana" checkinTime="1704939888436" checkinUser="felipe" docAuthor="Yang, Chengshuai, Li, Weichao, Li, Chen, Zhou, Zhihua, Xiao, Youli & Yan, Xing" docDate="2018" docId="783487C8CE279563FFEACFC96124F9FF" docLanguage="en" docName="Phytochemistry.155.83-92.pdf" docOrigin="Phytochemistry 155" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.07.009" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Ganoderma lucidum Karst" docType="treatment" docVersion="1" lastPageNumber="87" masterDocId="840DFFB0CE239567FF8EC91C644FFF8D" masterDocTitle="Metabolism of ganoderic acids by a Ganoderma lucidum cytochrome P 450 and the 3 - keto sterol reductase ERG 27 from yeast" masterLastPageNumber="92" masterPageNumber="83" pageNumber="87" updateTime="1705338592950" updateUser="juliana">
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<mods:title id="70B2DF8EA5C17925B42BA5A9C1CF7641">Metabolism of ganoderic acids by a Ganoderma lucidum cytochrome P 450 and the 3 - keto sterol reductase ERG 27 from yeast</mods:title>
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<mods:namePart id="AF8D3CD6DD12710B9F56E3FDDDC027EA">Yang, Chengshuai</mods:namePart>
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<mods:affiliation id="A7164EF8F8C1F16C99427FD46715D3D7">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China & ∗∗∗ & ∗∗ & University of Chinese Academy of Sciences, Beijing 100049, China</mods:affiliation>
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<mods:namePart id="65E3C3D9C13A9601E22E4B94F1C741A9">Li, Weichao</mods:namePart>
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<mods:affiliation id="683FEDC2F2A89CAA2B7928197ECB1A06">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China</mods:affiliation>
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<mods:namePart id="68598ADF2DF0FFCD8D6F586EC7EF77DE">Li, Chen</mods:namePart>
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<mods:affiliation id="6967A609A83AF4DC4FA5A1A9350383FB">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China</mods:affiliation>
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<mods:namePart id="5D5EBA9EF708A30E22293E6E98690093">Zhou, Zhihua</mods:namePart>
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<mods:affiliation id="E2D25DC778314A5A6C0E569C64289EA8">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China & ∗∗∗ & ∗∗ & University of Chinese Academy of Sciences, Beijing 100049, China</mods:affiliation>
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<mods:nameIdentifier id="B9A1FDDE6D56C20DEF1E9BB5402AA1DE" type="email">zhouzhihua@sippe.ac.cn</mods:nameIdentifier>
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<mods:namePart id="FB0A6B31C21ED55DF4FECC52531FC1AC">Xiao, Youli</mods:namePart>
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<mods:affiliation id="7BA24B2852108605365E93E00312E8A5">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China & ∗∗∗ & ∗∗ & University of Chinese Academy of Sciences, Beijing 100049, China</mods:affiliation>
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<mods:nameIdentifier id="3E4F0B6ED87C43679AE3E9412247A9B3" type="email">ylxiao@sibs.ac.cn</mods:nameIdentifier>
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<mods:namePart id="17B0BB0CF17C394B6D17FF4F361CE9F3">Yan, Xing</mods:namePart>
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<mods:affiliation id="E5F058A50C9EFD39C165B264588AD8C6">CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China</mods:affiliation>
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<mods:nameIdentifier id="4D7CCB1E5B76AA310D0CF096DB3739C6" type="email">yanxing@sibs.ac.cn</mods:nameIdentifier>
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<mods:title id="3D0EA3A86A0C78D62F2314115A94C247">Phytochemistry</mods:title>
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<mods:date id="8AA294E945596B1BC8A32FE774F712B7">2018</mods:date>
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<mods:number id="DA88176FA7DBC6E8686917CCC4DB2D21">2018-11-30</mods:number>
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<mods:number id="1535A024F91E9ACC7A2D15670CDB9482">155</mods:number>
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<mods:identifier id="18B6603CB881A114D99C88E78C1B88B5" type="DOI">10.1016/j.phytochem.2018.07.009</mods:identifier>
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<mods:identifier id="260981D9AA2122C40AA9EBE390A946A1" type="ISSN">1873-3700</mods:identifier>
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<treatment id="783487C8CE279563FFEACFC96124F9FF" LSID="urn:lsid:plazi:treatment:783487C8CE279563FFEACFC96124F9FF" httpUri="http://treatment.plazi.org/id/783487C8CE279563FFEACFC96124F9FF" lastPageNumber="87" pageId="4" pageNumber="87">
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<subSubSection id="B8876555CE279563FFEACFC96581F889" pageId="4" pageNumber="87" type="nomenclature">
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<paragraph id="F02236DECE279563FFEACFC96581F889" blockId="4.[100,721,1749,1796]" pageId="4" pageNumber="87">
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<heading id="AB6A81B2CE279563FFEACFC96581F889" bold="true" fontSize="36" level="1" pageId="4" pageNumber="87" reason="1">
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<emphasis id="C2E9EACCCE279563FFEACFC9669EF965" bold="true" box="[100,721,1749,1768]" italics="true" pageId="4" pageNumber="87">2.5. In vitro enzymatic activity confirmed the function of GLCPR, an</emphasis>
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<emphasis id="C2E9EACCCE279563FFEACFED6581F889" bold="true" box="[100,462,1777,1796]" italics="true" pageId="4" pageNumber="87">
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NADPH-CYP reductase from
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<taxonomicName id="379D4D5DCE279563FEE2CFED6581F889" ID-CoL="6JWYZ" authority="(Leyss. ex Fr.) Karst" authorityName="Karst" baseAuthorityName="Fr." box="[364,462,1777,1796]" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="4" pageNumber="87" phylum="Basidiomycota" rank="species" species="lucidum">G. lucidum</taxonomicName>
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</emphasis>
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</heading>
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</paragraph>
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<subSubSection id="B8876555CE279563FF0BCE356124F9FF" pageId="4" pageNumber="87" type="description">
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<paragraph id="F02236DECE279563FF0BCE356124F9FF" blockId="4.[100,771,1833,1992]" lastBlockId="4.[818,1488,1324,1650]" pageId="4" pageNumber="87">
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In general, a CYP requires an NADPH-CYP reductase for its function. In
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<taxonomicName id="379D4D5DCE279563FF0BCE5964B7F8D5" box="[133,248,1861,1880]" class="Tremellomycetes" family="Tremellaceae" genus="Saccharomyces" kingdom="Fungi" order="Tremellales" pageId="4" pageNumber="87" phylum="Basidiomycota" rank="species" species="cerevisiae">
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<emphasis id="C2E9EACCCE279563FF0BCE5964B7F8D5" bold="true" box="[133,248,1861,1880]" italics="true" pageId="4" pageNumber="87">S. cerevisiae</emphasis>
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</taxonomicName>
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, the NADPH-dependent CYP reductase, NCP1, was characterized as a working partner of endogenous or heterogeneous CYPs (
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<bibRefCitation id="940C4B2FCE279563FF2FCE616517F81D" author="Hirosue, S. & Tazaki, M. & Hiratsuka, N. & Yanai, S. & Kabumoto, H. & Shinkyo, R. & Arisawa, A. & Sakaki, T. & Tsunekawa, H. & Johdo, O. & Ichinose, H. & Wariishi, H." box="[161,344,1917,1936]" pageId="4" pageNumber="87" pagination="118 - 123" refId="ref9343" refString="Hirosue, S., Tazaki, M., Hiratsuka, N., Yanai, S., Kabumoto, H., Shinkyo, R., Arisawa, A., Sakaki, T., Tsunekawa, H., Johdo, O., Ichinose, H., Wariishi, H., 2011. Insight into functional diversity of cytochrome P 450 in the white-rot basidiomycete Phanerochaete chrysosporium: involvement of versatile monooxygenase. Biochem. Biophys. Res. Commun. 407, 118 - 123." type="journal article" year="2011">Hirosue et al., 2011</bibRefCitation>
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;
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<bibRefCitation id="940C4B2FCE279563FEEACE6165A0F81D" author="Ide, M. & Ichinose, H. & Wariishi, H." box="[356,495,1917,1936]" pageId="4" pageNumber="87" pagination="243 - 253" refId="ref9622" refString="Ide, M., Ichinose, H., Wariishi, H., 2012. Molecular identification and functional characterization of cytochrome P 450 monooxygenases from the brown-rot basidiomycete Postia placenta. Arch. Microbiol. 194, 243 - 253." type="journal article" year="2012">Ide et al., 2012</bibRefCitation>
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;
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<bibRefCitation id="940C4B2FCE279563FE74CE6166BBF81D" author="Venkateswarlu, K. & Kelly, D. E. & Kelly, S. L." box="[506,756,1917,1936]" pageId="4" pageNumber="87" pagination="776 - 780" refId="ref12231" refString="Venkateswarlu, K., Kelly, D. E., Kelly, S. L., 1997. Characterization of Saccharomyces cerevisiae CYP 51 and a CYP 51 fusion protein with NADPH cytochrome P- 450 oxidoreductase expressed in Escherichia coli. Antimicrob. Agents Chemother. 41, 776 - 780." type="journal article" year="1997">Venkateswarlu et al., 1997</bibRefCitation>
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). An ORF annotated as an NADPH-dependent CYP reductase, which shares a 36.62% amino acid sequence similarity with NCP1, was cloned from
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<taxonomicName id="379D4D5DCE279563FCEBCC306788FAB2" box="[869,967,1324,1343]" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="4" pageNumber="87" phylum="Basidiomycota" rank="species" species="lucidum">
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<emphasis id="C2E9EACCCE279563FCEBCC306788FAB2" bold="true" box="[869,967,1324,1343]" italics="true" pageId="4" pageNumber="87">G. lucidum</emphasis>
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</taxonomicName>
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cDNA and named
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<emphasis id="C2E9EACCCE279563FBF4CC306147FAB2" bold="true" box="[1146,1288,1324,1343]" italics="true" pageId="4" pageNumber="87">GLCPR. GLCPR</emphasis>
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was then introduced into a yeast chassis to replace the full-length ORF of
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<emphasis id="C2E9EACCCE279563FAA5CC546110FAD6" bold="true" box="[1323,1375,1352,1371]" italics="true" pageId="4" pageNumber="87">NCP1</emphasis>
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and the recombinant GLCPR113-3C strain was able to grow normally in YPD medium without the addition of ergosterol. It was demonstrated that the heterogenous GLCPR functioned in cooperation with endogenous CYPs in yeast to produce ergosterol, which is essential for yeast growth. Moreover, the pESC-dTrp-GLCPR-CYP512U6 expression vector containing the
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<emphasis id="C2E9EACCCE279563FC11CCEC604BF98E" bold="true" box="[927,1028,1520,1539]" italics="true" pageId="4" pageNumber="87">CYP512U6</emphasis>
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and
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<emphasis id="C2E9EACCCE279563FBBACCEC603AF98E" bold="true" box="[1076,1141,1520,1539]" italics="true" pageId="4" pageNumber="87">GLCPR</emphasis>
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ORFs under the control of the
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<emphasis id="C2E9EACCCE279563FA15CCEC6180F98E" bold="true" box="[1435,1487,1520,1539]" italics="true" pageId="4" pageNumber="87">GAL1</emphasis>
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and
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<emphasis id="C2E9EACCCE279563FCD2CF1067D3F992" bold="true" box="[860,924,1548,1567]" italics="true" pageId="4" pageNumber="87">GAL10</emphasis>
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promoters respectively, was constructed in GLCPR113-3C, and the resulting strain ZYGL02 catalyzed the conversion of ganoderic acid DM to
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<emphasis id="C2E9EACCCE279563FC2ECF5F67E3F9DB" bold="true" box="[928,940,1603,1622]" pageId="4" pageNumber="87">2</emphasis>
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(
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<figureCitation id="68A62A5BCE279563FC35CF5F67BEF9DB" box="[955,1009,1603,1622]" captionStart="Fig" captionStartId="5.[100,130,1574,1591]" captionTargetBox="[109,760,153,1550]" captionTargetId="figure-850@5.[108,761,152,1551]" captionTargetPageId="5" captionText="Fig. 5. Functional identification of GLCPR. Trace a shows the authentic ganoderic acid DM; Traces b, c, d and e show the analyses of products from the boiled microsomal fractions of ZYGL00, the microsomal fractions of ZYGL00, the microsomal fractions of ZYGL02, and the boiled microsomal fractions of ZYGL02 with ganoderic acid DM." figureDoi="http://doi.org/10.5281/zenodo.10484245" httpUri="https://zenodo.org/record/10484245/files/figure.png" pageId="4" pageNumber="87">Fig. 5</figureCitation>
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and
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<tableCitation id="BD1F0365CE279563FBAECF5F6028F9DB" box="[1056,1127,1603,1622]" captionStart="Table 1" captionStartId="1.[818,868,161,178]" captionTargetPageId="1" captionText="Table 1 Strains used in this study." pageId="4" pageNumber="87">Table 1</tableCitation>
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), indicating that the GLCPR functions in cooperation with CYPs such as CYP512U6, in
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<taxonomicName id="379D4D5DCE279563FA8DCF43612AF9FF" box="[1283,1381,1631,1650]" class="Agaricomycetes" family="Ganodermataceae" genus="Ganoderma" kingdom="Fungi" order="Polyporales" pageId="4" pageNumber="87" phylum="Basidiomycota" rank="species" species="lucidum">
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<emphasis id="C2E9EACCCE279563FA8DCF43612AF9FF" bold="true" box="[1283,1381,1631,1650]" italics="true" pageId="4" pageNumber="87">G. lucidum</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |