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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.99.90117" ID-Pensoft-Pub="1314-4049-99-45" ID-Pensoft-UUID="47D9DA3E1C56567CA14356476272F738" ModsDocID="1314-4049-99-45" checkinTime="1692098463846" checkinUser="pensoft" docAuthor="Itagaki, Hiyori &amp; Hosoya, Tsuyoshi" docDate="2023" docId="4AB365F9F86E5C048FEC494E9B4F6FAF" docLanguage="en" docName="MycoKeys 99: 45-85" docOrigin="MycoKeys 99" docPubDate="2023-08-14" docSource="http://dx.doi.org/10.3897/mycokeys.99.90117" docTitle="Neobelonopsis acutata Itagaki &amp; Hosoya 2023, sp. nov." docType="treatment" docVersion="1" id="47D9DA3E1C56567CA14356476272F738" lastPageNumber="45" masterDocId="47D9DA3E1C56567CA14356476272F738" masterDocTitle="A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan" masterLastPageNumber="85" masterPageNumber="45" pageNumber="45" updateTime="1692098463846" updateUser="pensoft">
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<mods:title id="4AB4E01EBD24455FAB5C9E06EF18A048">A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan</mods:title>
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<mods:namePart id="6A5FE6715411A69607BAADB883EBFBA1">Itagaki, Hiyori</mods:namePart>
<mods:nameIdentifier id="3646FDFDA8908B489DD307168082296F" type="ORCID">https://orcid.org/0000-0001-8678-0826</mods:nameIdentifier>
<mods:affiliation id="2BB4EE69C41321A4A0B7A002F8B560AC">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
<mods:nameIdentifier id="FDD5DCCF7A9CF7AC5CE1AD9730782274" type="email">itagaki@kahaku.go.jp</mods:nameIdentifier>
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<mods:namePart id="0A31125191F10DFD2898E8CB7CAE778A">Hosoya, Tsuyoshi</mods:namePart>
<mods:nameIdentifier id="DE1AE703F27D0751882293278F62DED9" type="ORCID">https://orcid.org/0000-0001-5360-5677</mods:nameIdentifier>
<mods:affiliation id="818071B312F49E66CA2C21474DA4E51E">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<treatment id="4AB365F9F86E5C048FEC494E9B4F6FAF" LSID="urn:lsid:plazi:treatment:4AB365F9F86E5C048FEC494E9B4F6FAF" httpUri="http://treatment.plazi.org/id/4AB365F9F86E5C048FEC494E9B4F6FAF" lastPageNumber="45" pageId="0" pageNumber="45">
<subSubSection id="FF03404C2FEBCF8437645FCC0DC568A7" pageId="0" pageNumber="45" type="nomenclature">
<paragraph id="6556625403D3CAB68A5CA72F03EEB01C" pageId="0" pageNumber="45">
<taxonomicName id="7675E6975648F8FE1E8628566BE8D3D2" LSID="4AB365F9-F86E-5C04-8FEC-494E9B4F6FAF" authority="Itagaki &amp; Hosoya" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" higherTaxonomySource="treatment-meta" kingdom="Fungi" lsidName="Neobelonopsis acutata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="acutata" status="sp. nov.">Neobelonopsis acutata Itagaki &amp; Hosoya</taxonomicName>
<taxonomicNameLabel id="EC38F2554D3896119171B6686D3CE71D" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="4F26DCA56FF140303A45DE3D75605D75" pageId="0" pageNumber="45" type="description">
<paragraph id="962D1852C893F2557DC341685EA5B3E8" pageId="0" pageNumber="45">
<figureCitation id="9183A4D1F2487D624D892B57BBFDB0BE" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Neobelonopsis acutata (TNS-F- 86648, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia C stromata with sparse subiculum D one month old colony on PDA E vertical section of the apothecium (in LA) F refractive vacuoles in fresh marginal cells (in water) G asci with ascospores (in CB / LA) H paraphyses (in CB / LA) I paraphyses with a long refractive vacuole (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L ascospores (in CB / LA) M conidia (in water) N, O conidiophores (in water) P bulbile (in CB / LA) Q subicular hyphae (in CB / LA). Scale bars: 1 mm (A, B); 0.2 mm (C); 25 μm (E, Q); 10 μm (F-P)." figureDoi="10.3897/mycokeys.99.90117.figure3" httpUri="https://binary.pensoft.net/fig/892419" pageId="0" pageNumber="45">Figs 3</figureCitation>
<figureCitation id="A3234625E934BAE8858D8239F777B9DE" captionStart="Figure 13" captionStartId="F13" captionText="Figure 13. Line-drawings of the Neobelonopsis and Trichobelonium species I vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium II asci III paraphyses IV ascospores. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure13" httpUri="https://binary.pensoft.net/fig/892429" pageId="0" pageNumber="45">, 13</figureCitation>
<figureCitation id="8BD1A9E3D7A2BD4151A82FF2DAC18381" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">, 14A</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="C1ED659CCD78319EB37C4213216C05BD" pageId="0" pageNumber="45" type="etymology">
<paragraph id="E8BFA7F7B431946F231E742457E1FD3B" pageId="0" pageNumber="45">Etymology.</paragraph>
<paragraph id="65EF65D7A5C5EA274C91B91A2487F073" pageId="0" pageNumber="45">Named after the acute apices of ascospores.</paragraph>
</subSubSection>
<subSubSection id="15976CF75CB1AC52954CEEA2ECDD6EE1" pageId="0" pageNumber="45" type="diagnosis">
<paragraph id="04D0BC7E06499A88BCB7773C4FB87749" pageId="0" pageNumber="45">Diagnosis.</paragraph>
<paragraph id="82D5671F02B3D3F396DB4F831442BB55" pageId="0" pageNumber="45">
Characterized by 3-septate ascospore with acute extremes and conidiophores densely aggregated in clusters. The present species resembles
<taxonomicName id="71F134C0717AAD0C0986850917ACB579" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. multiguttata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="multiguttata">
<emphasis id="08F24DBE682BBB5DD4A6B217DB652847" italics="true" pageId="0" pageNumber="45">N. multiguttata</emphasis>
</taxonomicName>
. See Diagnosis in
<taxonomicName id="F3131B727D836CD46875E21A49618667" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. multiguttata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="multiguttata">
<emphasis id="0BB97E86660E9D286910ED32A997DB3A" italics="true" pageId="0" pageNumber="45">N. multiguttata</emphasis>
</taxonomicName>
for diagnostic characters.
</paragraph>
</subSubSection>
<subSubSection id="056607FBA483746036A94EDDF2528F66" pageId="0" pageNumber="45" type="holotype">
<paragraph id="CACF32B741DB12223A22E42F8181E6C8" pageId="0" pageNumber="45">Holotype.</paragraph>
<paragraph id="28CB55035C83474DC04423C80A29B6CD" pageId="0" pageNumber="45">
TNS-F-86648, Yugashima, Izu City, Shizuoka Pref. Japan, 15 October 2021, on decaying culm of
<taxonomicName id="EB3BC1339DF04957299370C3D07BDE01" class="Ascomycetes" family="Hyaloscyphaceae" genus="Miscanthus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Miscanthus sinensis" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sinensis">
<emphasis id="3FD0F1A677850C0624DEF5CDD91261FF" italics="true" pageId="0" pageNumber="45">Miscanthus sinensis</emphasis>
</taxonomicName>
; ex-holotype culture NBRC 115570.
</paragraph>
</subSubSection>
<subSubSection id="5A196D9AEFFB5D9EE470C8B7E311ADB3" pageId="0" pageNumber="45" type="description">
<paragraph id="082EDDBA80EC1A0E8BE61F9421A3EF32" pageId="0" pageNumber="45">Description.</paragraph>
<paragraph id="1D520058F15BE87108160B22E4505DDB" pageId="0" pageNumber="45">
<emphasis id="F7D9209E7DA2884722981BE926572ED4" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
arising from scuta.
<emphasis id="257C5FB988225174E56685CC3A12EAEC" bold="true" italics="true" pageId="0" pageNumber="45">Scuta</emphasis>
superficial, scattered to gregarious, flat discoid, blackish brown (C80M100Y80-100K60), 0.1-0.3 mm diam.,
<emphasis id="864A87B446CBBCBBADE83FBA8BCA4545" bold="true" italics="true" pageId="0" pageNumber="45">textura epidermoidea</emphasis>
, composed of closely packed thick-walled cells.
<emphasis id="643CE71FEB35639B9C35837EC1C0DECA" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
0.1-0.2 mm high, seated on subiculum, with grayish brown (C0-30M30Y40K60) to black receptacle; disc 0.25-1.4 mm diam., white to pale gray (K10) when fresh, shrunk to 0.2-1 mm diam., turns pale yellow (Y10) when dried. Ectal excipulum 25-40
<normalizedToken id="F3A135B2A1A0E0F02D8EA7CB1F613201" originalValue="µm">µm</normalizedToken>
thick at base, 15-20
<normalizedToken id="A9EB4653F1008FC3438C98EA59A1E97A" originalValue="µm">µm</normalizedToken>
thick at the upper flank to margin; cortical cells hemispherical to pyriform, 14-16
<normalizedToken id="11C4C68473851D60DF6EA6041E066021" originalValue="×">x</normalizedToken>
9-11
<normalizedToken id="215FB91B2F8068492F4FB858B80708CF" originalValue="µm">µm</normalizedToken>
at base, becoming smaller to 10-12
<normalizedToken id="2B4CBA7668DFF62C27CCC1677EA05DF0" originalValue="×">x</normalizedToken>
7-9
<normalizedToken id="236A78CD19F4777907970ACBEC5F0DE3" originalValue="µm">µm</normalizedToken>
toward the upper flank to margin, containing refractive vacuoles in the protruding cells when mounted fresh in water. Medullary excipulum 25-50
<normalizedToken id="2D482C54CC995553F42E6110D7F6D92D" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="558B6B007D1AA1D8C177D665B3A40C18" bold="true" italics="true" pageId="0" pageNumber="45">Asci</emphasis>
(50-)65-82(-85)
<normalizedToken id="AE862B578D907B1EC1748E4D0350ED5A" originalValue="×">x</normalizedToken>
5-9
<normalizedToken id="200351BD4E42BA14BA20A8963578489E" originalValue="µm">µm</normalizedToken>
, arising from croziers, with MLZ + apical pore.
<emphasis id="47EE5DD2E89E0EF6D682B3213A6098E8" bold="true" italics="true" pageId="0" pageNumber="45">Ascospores</emphasis>
15-22(-27.5)
<normalizedToken id="A0EFB692AB42BAAE4B266F8099BB5B9B" originalValue="×">x</normalizedToken>
2.5-3.5
<normalizedToken id="DF18158DB8346CCBC8A553532CEAAFDB" originalValue="µm">µm</normalizedToken>
, long fusiform, with acute apices, (1-)3(-4)-septate, containing abundant guttules, often 2-3(-4) large guttules and several smaller ones.
<emphasis id="189B2E0070FA8E46076FBE6CC683774A" bold="true" italics="true" pageId="0" pageNumber="45">Paraphyses</emphasis>
(65-)70-85(-93)
<normalizedToken id="3FF965BA23F24CD41EF0C7A09799D2E3" originalValue="×">x</normalizedToken>
2.5-3
<normalizedToken id="565F51ACC225A16126B8325DF2B726EB" originalValue="µm">µm</normalizedToken>
, simple, 2-3-septate, apical cells containing long refractive vacuoles when mounted fresh in water.
<emphasis id="720F00CF6891C28F1D3E9B54AE58F7BA" bold="true" italics="true" pageId="0" pageNumber="45">Subiculum</emphasis>
thinly covering the surface of substrates in patches, sparse to moderately abundant around the scuta and apothecia, shiny brown; subicular hyphae straight to curved, usually 3-5
<normalizedToken id="98C0A8E6499D0A057F7D1FB999F983A9" originalValue="µm">µm</normalizedToken>
diam., with 0.5-1
<normalizedToken id="FC8B6D62E117554F47C6623D5DDAC2B2" originalValue="µm">µm</normalizedToken>
thick-walls, septate every 15-25
<normalizedToken id="9308D368E658992FC6BC6E101D296C5C" originalValue="µm">µm</normalizedToken>
, perpendicularly branched, covered by gelatinous substance, forming bulbils of 30-45
<normalizedToken id="8499651135ABF6381F358D4B0C7DBB37" originalValue="µm">µm</normalizedToken>
across in the middle or tip, composed of densely aggregated globular or moniliform thick-walled cells, dark brown.
<emphasis id="E89A16C04F61F2438E74B442649B6700" bold="true" italics="true" pageId="0" pageNumber="45">Colony</emphasis>
of NBRC 115570 on PDA moderately undulate, superficial, cottony to hairy, brownish gray (C20-40M40Y40K60) from the surface, zonation only observed from the reverse, without soluble pigment and crystals; aerial mycelium densely fascicular, white.
<emphasis id="8EAA465EB8DFA9473FCB8DAE08CE2EEB" bold="true" italics="true" pageId="0" pageNumber="45">Conidiophores</emphasis>
aggregated in inconspicuous clusters on aerial hyphae, (semi-)macronematous, constricted, arising vertically or laterally from hyphae, pale to dark brown, smooth, thick-walled, frequently branched;
<emphasis id="79E2128A9A32A55CFDD67793023ED6DF" bold="true" italics="true" pageId="0" pageNumber="45">phialides</emphasis>
ampulliform with determinate collarettes, up to 15
<normalizedToken id="CF6D7E2A1A3A4380704782C6B7F81570" originalValue="µm">µm</normalizedToken>
long, approximately 4
<normalizedToken id="FEFD8067F9DA1E135BEBCFCD1A2EECC1" originalValue="µm">µm</normalizedToken>
width at base, discrete to integrated, terminal or intercalary, pale brown, thick-walled, with cylindrical to wide funnel-shape collarettes of 5-8
<normalizedToken id="A9D672F9C1267C34C84F18A4F925AEC2" originalValue="×">x</normalizedToken>
2.5-3
<normalizedToken id="E3963C0C0F18F1E9D4D912C018BE10BC" originalValue="µm">µm</normalizedToken>
;
<emphasis id="F1EB2134E1D6DA09F8B3B28596A08134" bold="true" italics="true" pageId="0" pageNumber="45">conidia</emphasis>
aseptate, spherical to subspherical, 2-2.5
<normalizedToken id="F92245C6217849856806FF26B7F18B4D" originalValue="µm">µm</normalizedToken>
diam., hyaline, thin-walled.
</paragraph>
</subSubSection>
<subSubSection id="4215178B799F992BCAC15D90AD5BC26A" pageId="0" pageNumber="45" type="additional specimen examined">
<paragraph id="EED5A2A466854F68D0D3CD5D53B9C446" pageId="0" pageNumber="45">Additional specimen examined.</paragraph>
<paragraph id="66B120A114242D09D353C1B8306EEF35" pageId="0" pageNumber="45">
TNS-F-86671, Kawazu City, Kamo County, Shizuoka Pref., 16 October 2021, on decaying culm of
<taxonomicName id="0F34A380668B710CEE72D03ABC003E58" lsidName="M. sinensis" pageId="0" pageNumber="45" rank="species" species="sinensis">
<emphasis id="9339A492297F82817410ADCF0FD9CF36" italics="true" pageId="0" pageNumber="45">M. sinensis</emphasis>
</taxonomicName>
, culture NBRC 115666.
</paragraph>
</subSubSection>
<subSubSection id="AF3FF7814069859EE571FDB32A40BC20" pageId="0" pageNumber="45" type="notes">
<paragraph id="8FA8D75FADED7EDB9D683AA7E3DD46EF" pageId="0" pageNumber="45">Notes.</paragraph>
<paragraph id="1F07E733FF8A0A0E9FFEDB48AF87590A" pageId="0" pageNumber="45">
<taxonomicName id="45EE58444B2C17D28F729407AA744FFA" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis acutata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="acutata">
<emphasis id="302DA57F63AEE35258975C184035A28F" italics="true" pageId="0" pageNumber="45">Neobelonopsis acutata</emphasis>
</taxonomicName>
resembles
<taxonomicName id="966E0230F5E733AFD6C65129C21285A7" class="Ascomycetes" family="Dermateoideae" genus="Belonopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Belonopsis graminea" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="graminea">
<emphasis id="5D3F900EE7184270CF6C19CEDA362A5F" italics="true" pageId="0" pageNumber="45">Belonopsis graminea</emphasis>
</taxonomicName>
(P. Karst.) Sacc. &amp; P. Syd., which has a whitish disc that turns yellowish when dried, asci, ascospores and paraphyses with overlapped biometry (
<bibRefCitation id="40B0B4C2CAFEE8EB92C05381BB42638B" author="Karsten, PA" journalOrPublisher="Nature Methods" pageId="0" pageNumber="45" refId="B22" refString="Karsten, PA, 1871. Mycologia Fennica. Pars prima. Discomycetes. Bidrag Till Kaennedom Af Finlands Natur Och Folk, utgifna af Finska Vetenskaps-Societeten, 19: 1-264. Finska Litteratur-saellskapets tryckeri, Helsingfors." title="Mycologia Fennica. Pars prima. Discomycetes. Bidrag Till Kaennedom Af Finlands Natur Och Folk, utgifna af Finska Vetenskaps-Societeten, 19: 1 - 264. Finska Litteratur-saellskapets tryckeri, Helsingfors." year="1871">Karsten 1871</bibRefCitation>
). However,
<taxonomicName id="C129AD64949F1F1696CED5F90FB8D242" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
<emphasis id="862F7A6F65FFEB0D3556DB64D5643D5D" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
</taxonomicName>
differs from
<taxonomicName id="BA34AB41475D7E7A64C48A6E5CAFCDCC" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="B. graminea" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="graminea">
<emphasis id="04BABC695F46C283C92DE7F990A795B7" italics="true" pageId="0" pageNumber="45">B. graminea</emphasis>
</taxonomicName>
in its amyloid asci.
<taxonomicName id="75913C6E8EE611E98F297AACD685891A" class="Ascomycetes" family="Dermateoideae" genus="Belonopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Belonopsis graminea" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="graminea">
<emphasis id="C084E331A21951E3446FD12AC64CD9D6" italics="true" pageId="0" pageNumber="45">Belonopsis graminea</emphasis>
</taxonomicName>
produces densely aggregated conidiophores (approximately 0.2 mm across,
<normalizedToken id="BD642BB68DCBF02F6F8DF2593CC34B81" originalValue="“spermogonium”">&quot;spermogonium&quot;</normalizedToken>
sensu Karsten) and cylindrical to elongated fusiform conidia (8-10
<normalizedToken id="652B00DEE49AFBA4E89212B4E8CF3836" originalValue="×">x</normalizedToken>
1.5
<normalizedToken id="7100D9BD773A3BA509C08539EEC1EBE6" originalValue="µm">µm</normalizedToken>
,
<normalizedToken id="6BF889109A7ADECF7586F80F473EAC2C" originalValue="“spermatia”">&quot;spermatia&quot;</normalizedToken>
sensu Karsten) (
<bibRefCitation id="ACBDCFEB0284A9B2BE4AF93F96358477" author="Karsten, PA" journalOrPublisher="Nature Methods" pageId="0" pageNumber="45" refId="B22" refString="Karsten, PA, 1871. Mycologia Fennica. Pars prima. Discomycetes. Bidrag Till Kaennedom Af Finlands Natur Och Folk, utgifna af Finska Vetenskaps-Societeten, 19: 1-264. Finska Litteratur-saellskapets tryckeri, Helsingfors." title="Mycologia Fennica. Pars prima. Discomycetes. Bidrag Till Kaennedom Af Finlands Natur Och Folk, utgifna af Finska Vetenskaps-Societeten, 19: 1 - 264. Finska Litteratur-saellskapets tryckeri, Helsingfors." year="1871">Karsten 1871</bibRefCitation>
), while
<taxonomicName id="1AB3331D037650A8998D94B9651AEB68" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
<emphasis id="C5B8F22CEFAE1578ED1F5270565DF332" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
</taxonomicName>
has sparsely aggregated conidiophores and spherical conidia (Figs
<figureCitation id="720CEE9A15E868CCAF6B209435885321" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Neobelonopsis acutata (TNS-F- 86648, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia C stromata with sparse subiculum D one month old colony on PDA E vertical section of the apothecium (in LA) F refractive vacuoles in fresh marginal cells (in water) G asci with ascospores (in CB / LA) H paraphyses (in CB / LA) I paraphyses with a long refractive vacuole (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L ascospores (in CB / LA) M conidia (in water) N, O conidiophores (in water) P bulbile (in CB / LA) Q subicular hyphae (in CB / LA). Scale bars: 1 mm (A, B); 0.2 mm (C); 25 μm (E, Q); 10 μm (F-P)." figureDoi="10.3897/mycokeys.99.90117.figure3" httpUri="https://binary.pensoft.net/fig/892419" pageId="0" pageNumber="45">3M</figureCitation>
,
<figureCitation id="9D644777BF062C04156E29941F40BF71" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">14A</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>