treatments-xml/data/E8/E9/6E/E8E96E07A73357DABBA5B555754A3F57.xml

<document ID-DOI="http://dx.doi.org/10.3897/phytokeys.65.6726" ID-PMC="PMC4957022" ID-Pensoft-Pub="1314-2003-65-47" ID-Pensoft-UUID="FFA74860FF9BFFCDFFB2FFA5FFFFFFF4" ID-PubMed="27489488" ID-Zenodo-Dep="122987" ModsDocID="1314-2003-65-47" checkinTime="1466061698095" checkinUser="pensoft" docAuthor="Toyama, Hironori, Tagane, Shuichiro, Dang, Van Son, Tran, Hop, Nagamasu, Hidetoshi, Naiki, Akiyo &amp; Yahara, Tetsukazu" docDate="2016" docId="E8E96E07A73357DABBA5B555754A3F57" docLanguage="en" docName="PhytoKeys 65: 47-55" docOrigin="PhytoKeys 65" docPubDate="2016-06-15" docSource="http://dx.doi.org/10.3897/phytokeys.65.6726" docTitle="Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang 2016, sp. nov." docType="treatment" docVersion="3" id="FFA74860FF9BFFCDFFB2FFA5FFFFFFF4" lastPageNumber="51" masterDocId="FFA74860FF9BFFCDFFB2FFA5FFFFFFF4" masterDocTitle="A new species of Eustigma (Hamamelidaceae) from Hon Ba Nature Reserve, Vietnam" masterLastPageNumber="55" masterPageNumber="47" pageNumber="49" updateTime="1668141724948" updateUser="ExternalLinkService">
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<mods:title>A new species of Eustigma (Hamamelidaceae) from Hon Ba Nature Reserve, Vietnam</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Toyama, Hironori</mods:namePart>
<mods:affiliation>Center for Asian Conservation Ecology, Kyushu University, 744 Motooka, Fukuoka, 819 - 0395, Japan</mods:affiliation>
<mods:nameIdentifier type="email">htoyascb@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tagane, Shuichiro</mods:namePart>
<mods:affiliation>Center for Asian Conservation Ecology, Kyushu University, 744 Motooka, Fukuoka, 819 - 0395, Japan</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Dang, Van Son</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tran, Hop</mods:namePart>
<mods:affiliation>University of Science Ho Chi Minh City, 227 Nguyen Van Cu Street, District 5, Ho Chi Minh City, Vietnam</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Nagamasu, Hidetoshi</mods:namePart>
<mods:affiliation>The Kyoto UniversityMuseum, Kyoto University, Yoshida Honmachi, Sakyo-ku, Kyoto, 606 - 8501, Japan</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Naiki, Akiyo</mods:namePart>
<mods:affiliation>Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Taketomi-cho, Yaeyama-gun, Okinawa, 907 - 1541, Japan</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yahara, Tetsukazu</mods:namePart>
<mods:affiliation>Center for Asian Conservation Ecology, Kyushu University, 744 Motooka, Fukuoka, 819 - 0395, Japan</mods:affiliation>
</mods:name>
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<mods:date>2016</mods:date>
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<mods:number>2016-06-15</mods:number>
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<mods:number>65</mods:number>
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<treatment ID-GBIF-Taxon="182227033" LSID="urn:lsid:plazi:treatment:E8E96E07A73357DABBA5B555754A3F57" httpUri="http://treatment.plazi.org/id/E8E96E07A73357DABBA5B555754A3F57" lastPageId="4" lastPageNumber="51" pageId="2" pageNumber="49">
<subSubSection pageId="2" pageNumber="49" type="nomenclature">
<paragraph pageId="2" pageNumber="49">
<taxonomicName LSID="E8E96E07-A733-57DA-BBA5-B555754A3F57" authority="H. Toyama, Tagane &amp; V. S. Dang" authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="2" pageNumber="49" phylum="Tracheophyta" rank="species" species="honbaense" status="sp. nov.">Eustigma honbaense H.Toyama, Tagane &amp; V.S.Dang</taxonomicName>
<taxonomicNameLabel pageId="2" pageNumber="49">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A capsule; B immature fruits (lateral view, longitudinal and transverse sections from left) C, axillary bud (ab), bud scale (bs), petiole (pt) and stipule (st) and uppermost branch (ub) D bracteole E fruiting branch F vein with stellate hair G young branch H stellate hair on bud. [A-H from Tagane et al. V 1586, KYO. Drawn by H. Toyama.]" figureDoi="10.3897/phytokeys.65.6726.figure2" httpUri="https://binary.pensoft.net/fig/92604" pageId="2" pageNumber="49">Figs 2</figureCitation>
<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A branch with infructescence B fruits; C abaxial surface of leaf D young infructescence E lamina showing glabrous abaxial surface F seeds with a large hilum showing basal side on the placenta (upper), and apical side (lower). [A-D photographed on 14 July 2014, E &amp; F from Tagane et al. V 1586, KYO.]" figureDoi="10.3897/phytokeys.65.6726.figure3" httpUri="https://binary.pensoft.net/fig/92605" pageId="2" pageNumber="49">, 3</figureCitation>
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="2" pageNumber="49">, 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="49" type="diagnosis">
<paragraph pageId="2" pageNumber="49">Diagnosis.</paragraph>
<paragraph pageId="2" pageNumber="49">
This species is similar to 
<taxonomicName authorityName="Gardner &amp; Champ" authorityYear="1849" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma oblongifolium" order="Saxifragales" pageId="2" pageNumber="49" phylum="Tracheophyta" rank="species" species="oblongifolium">
<emphasis italics="true" pageId="2" pageNumber="49">Eustigma oblongifolium</emphasis>
</taxonomicName>
Gardner &amp; Champ., but distinguished from that species by having entire elliptic to oblong leaves (vs. partly dentate oblong-lanceolate leaves), infructescences 5-10.3 cm long (vs. 3-5 cm long), apical part (above tepal scar) of capsules 6.5-9 mm long (vs. 3-5 mm long) and seed hilum 4 mm long and 3 mm wide for bigger side (vs. 2-3 mm long and 1-1.5 mm wide).
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="49" type="materials_examined">
<paragraph pageId="2" pageNumber="49">
<typeStatus>Type</typeStatus>
.
</paragraph>
<paragraph pageId="2" pageNumber="49">
<materialsCitation collectingDate="2014-07-14" collectorName="S. Tagane, H. Kanemitsu, V. S. Dang, H. Tran, X. N. Loi, N. D. Thach, N. Dinh, H. N. P. Hieu" country="VIETNAM" elevation="401" latitude="12.1095" location="Hon Ba Nature Reserve" longLatPrecision="13" longitude="108.98683" municipality="Hon Ba Nature Reserve" specimenCount="1" stateProvince="Khanh Hoa Province" typeStatus="holotype">
<collectingCountry name="Vietnam">VIETNAM</collectingCountry>
. 
<collectingRegion country="Vietnam" name="Khanh Hoa">Khanh Hoa Province</collectingRegion>
, 
<collectingMunicipality>Hon Ba Nature Reserve</collectingMunicipality>
, evergreen forest margin along streamside, 
<quantity metricMagnitude="2" metricUnit="m" metricValue="4.01" unit="m" value="401.0">
<elevation metricMagnitude="2" metricUnit="m" metricValue="4.01" unit="m" value="401.0">401 m</elevation>
</quantity>
alt., 
<geoCoordinate degrees="12" direction="north" minutes="06.57" orientation="latitude" precision="9" value="12.1095">12°06.57'N</geoCoordinate>
, 
<geoCoordinate degrees="108" direction="east" minutes="59.21" orientation="longitude" precision="9" value="108.98683">108°59.21'E</geoCoordinate>
(DDM), 
<collectingDate value="2014-07-14">14 July 2014</collectingDate>
, 
<emphasis italics="true" pageId="2" pageNumber="49">
<collectorName>S. Tagane</collectorName>
, 
<collectorName>H. Kanemitsu</collectorName>
, 
<collectorName>V.S. Dang</collectorName>
</emphasis>
, 
<emphasis italics="true" pageId="2" pageNumber="49">
<collectorName>H. Tran</collectorName>
</emphasis>
, 
<emphasis italics="true" pageId="2" pageNumber="49">
<collectorName>X.N. Loi</collectorName>
, 
<collectorName>N.D. Thach</collectorName>
</emphasis>
, 
<emphasis italics="true" pageId="2" pageNumber="49">
<collectorName>N. Dinh</collectorName>
&amp; 
<collectorName>H.N.P. Hieu</collectorName>
<collectingDate value="1586-05">V1586</collectingDate>
</emphasis>
(
<typeStatus>holotype</typeStatus>
: KYO!; isotypes: FU!, VNM!, the herbarium of 
<location LSID="urn:lsid:plazi:treatment:E8E96E07A73357DABBA5B555754A3F57:C586EDF1463F31B23E8FB3D8973C2EE0" country="VIETNAM" latitude="12.1095" longLatPrecision="13" longitude="108.98683" municipality="Hon Ba Nature Reserve" name="Hon Ba Nature Reserve" stateProvince="Khanh Hoa Province">Hon Ba Nature Reserve</location>
!)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection lastPageId="4" lastPageNumber="51" pageId="2" pageNumber="49" type="description">
<paragraph pageId="2" pageNumber="49">Description.</paragraph>
<paragraph lastPageId="4" lastPageNumber="51" pageId="2" pageNumber="49">
Trees 8-10 m tall. Branches yellow-green when young, turning brown when mature; indumentum sparsely to densely brown stellate hairy when young (denser on the uppermost branch), glabrescent; lenticels more distinct on young branches when dry. Terminal buds naked, narrowly ovoid, ca. 5 mm long, ca. 2 mm wide, having 2 opposite stipules incompletely covering young leaves, linear, ca. 4.5 mm long, ca. 0.5 mm wide, brown stellate hairy. Axillary buds scaled, narrowly ovoid, ca. 3.5 mm long, ca. 1.2 mm wide; bud scales 2, opposite, incompletely covering young leaves, narrowly ovate-oblong, 3-4 mm long, 0.5-1 mm wide, densely brown stellate hairy. Leaf blades elliptic to oblong, 6.5-21.5 cm long, 2.2-8.5 cm wide, coriaceous, lustrous and glabrous on both surfaces except veins below, base obtuse to cuneate, apex acuminate to rounded, margin entire; midribs sunken and glabrous above, prominent and sparsely brown stellate hairy below; secondary veins 5-9 pairs, slightly sunken and glabrous above, prominent and sparsely brown stellate hairy below; tertiary veins reticulate, slightly sunken and glabrous above, prominent and sparsely brown stellate hairy below; petiole 9-14 mm long, sparsely brown stellate hairy; stipules 2, opposite, caducous, linear, 5-8 mm long, 0.5-1 mm wide, brown stellate hairy. Flowers not seen. Infructescences terminal and axillary, racemose, 5-10.3 cm long; peduncles 1.2-4.5 cm long, brown stellate hairy, with 0-2 basal leaves; basal bracts not seen; bracts and bracteoles caducous, narrowly ovate, 1.8-3 mm long, 0.5-1 mm wide (but see note), densely brown stellate hairy; fruiting pedicels 5-8 mm long, densely brown stellate hairy. Floral cups in young fruits, turbinate, 2-3 mm in diameter, densely 
<pageBreakToken pageId="3" pageNumber="50" start="start">brown</pageBreakToken>
stellate hairy; ovary 2-locular; ovules 1 per locule. Capsules ovoid-globular, 12-16 mm long, 9-10 mm in diameter, woody, dehiscing loculicidally by two 2-lobed valves, sparsely lenticellate, sparsely brown stellate hairy, the length above tepal scar 
<pageBreakToken pageId="4" pageNumber="51" start="start">6.5</pageBreakToken>
-9 mm; endocarp loose from woody exocarp. Seeds 2 per capsule, narrowly ovoid, ca. 10 mm long, ca. 4.5 mm wide, ca. 3 mm thick, brownish black, smooth, hilum ca. 4 mm long, ca. 3 mm wide for bigger side (the basal side on the placenta).
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="other specimen examined">
<paragraph pageId="4" pageNumber="51">Other specimen examined.</paragraph>
<paragraph pageId="4" pageNumber="51">
VIETNAM. Khanh Hoa Province, Hon Ba Nature Reserve, evergreen forest margin along streamside, 393 m alt., 
<geoCoordinate degrees="12" direction="north" minutes="06.51" orientation="latitude" precision="9" value="12.1085">12°06.51'N</geoCoordinate>
, 
<geoCoordinate degrees="108" direction="east" minutes="59.26" orientation="longitude" precision="9" value="108.98766">108°59.26'E</geoCoordinate>
(DDM), 22 November 2014, 
<emphasis italics="true" pageId="4" pageNumber="51">H. Toyama, S. Tagane, V.S. Dang, H. Nagamasu, A. Naiki, H. Tran, C.J. Yang, H.Q. Cuong &amp; H.N.P. Hieu V1975</emphasis>
(FU!, VNM!, the herbarium of Hon Ba Nature Reserve!).
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="distribution">
<paragraph pageId="4" pageNumber="51">Distribution and habit.</paragraph>
<paragraph pageId="4" pageNumber="51">
This species is only known from Hon Ba Nature Reserve of southern Vietnam. We found only three individuals in the evergreen forest along a stream at ca. 400 m alt. The flora of this area is reported in 
<bibRefCitation author="Choudhary, RK" journalOrPublisher="Annales Botanici Fennici" pageId="5" pageNumber="52" pagination="369 - 376" publicationUrl="10.5735/085.049.0608" refId="B2" refString="Choudhary, RK, Bach, TT, Nong, LV, Hai, DV, Quang, BH, Lee, YM, Park, SH, Lee, C, Lee, J, 2012. Dillenia tetrapetala (Dilleniaceae), a new species from HonBa Nature Reserve, Vietnam. Annales Botanici Fennici 49: 369 - 376, DOI: 10.5735/085.049.0608" title="Dillenia tetrapetala (Dilleniaceae), a new species from HonBa Nature Reserve, Vietnam." url="10.5735/085.049.0608" volume="49" year="2012">Choudhary et al. (2012)</bibRefCitation>
, 
<bibRefCitation author="Schuiteman, A" journalOrPublisher="OrchideenJournal" pageId="6" pageNumber="53" pagination="10 - 16" refId="B13" refString="Schuiteman, A, Averyanov, L, Rybkova, R, 2013. Vanilla atropogon, a new species from Vietnam. OrchideenJournal 1: 10 - 16" title="Vanilla atropogon, a new species from Vietnam." volume="1" year="2013">Schuiteman et al. (2013)</bibRefCitation>
and 
<bibRefCitation author="Tagane, S" journalOrPublisher="PhytoKeys" pageId="6" pageNumber="53" pagination="1 - 8" publicationUrl="10.3897/phytokeys.50.4427" refId="B14" refString="Tagane, S, Dang, S, Yahara, T, Toyama, H, Tran, H, 2015a. Goniothalamus flagellistylus Tagane &amp; V.S.Dang, (Annonaceae), a new species from Mt. Hon Ba, Vietnam. PhytoKeys 50: 1 - 8, DOI: 10.3897/phytokeys.50.4427" title="Goniothalamus flagellistylus Tagane &amp; V. S. Dang, (Annonaceae), a new species from Mt. Hon Ba, Vietnam." url="10.3897/phytokeys.50.4427" volume="50" year="2015 a">Tagane et al. (2015a</bibRefCitation>
, 
<bibRefCitation author="Tagane, S" journalOrPublisher="PhytoKeys" pageId="6" pageNumber="53" pagination="51 - 60" publicationUrl="10.3897/phytokeys.57.6347" refId="B15" refString="Tagane, S, Dang, VS, Toyama, H, Naiki, A, Nagamasu, H, Yahara, T, Tran, H, 2015b. Aporosa tetragona Tagane &amp; V.S.Dang (Phyllanthaceae), a new species from Mt. Hon Ba, Vietnam. PhytoKeys 57: 51 - 60, DOI: 10.3897/phytokeys.57.6347" title="Aporosa tetragona Tagane &amp; V. S. Dang (Phyllanthaceae), a new species from Mt. Hon Ba, Vietnam." url="10.3897/phytokeys.57.6347" volume="57" year="2015 b">b</bibRefCitation>
).
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="phenology">
<paragraph pageId="4" pageNumber="51">Phenology.</paragraph>
<paragraph pageId="4" pageNumber="51">Flowering season is unknown. Immature fruits and capsules were observed in July and November.</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="etymology">
<paragraph pageId="4" pageNumber="51">Etymology.</paragraph>
<paragraph pageId="4" pageNumber="51">
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
is named after the type locality, Hon Ba Nature Reserve.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="preliminary conservation status">
<paragraph pageId="4" pageNumber="51">Preliminary conservation status.</paragraph>
<paragraph pageId="4" pageNumber="51">
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
was collected from a single locality in the Hon Ba Nature Reserve, where only three individuals (one reproductive tree and two young trees) were found in evergreen forest along a streamside. The forest around the habitat was frequently logged and disturbed. Therefore, this species is assessed as Critically Endangered (CR) using the criterion D of the Red List Categories (
<bibRefCitation author="IUCN" journalOrPublisher="Gland, Switzerland" pageId="6" pageNumber="53" pagination="1 - 32" refId="B8" refString="IUCN, 2012. IUCN Red List Categories and Criteria. Version 3.1. Gland, Switzerland: 1 - 32" title="IUCN Red List Categories and Criteria. Version 3.1" year="2012">IUCN 2012</bibRefCitation>
), although more individuals could be discovered by more thorough surveys.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="notes">
<paragraph pageId="4" pageNumber="51">Note.</paragraph>
<paragraph pageId="4" pageNumber="51">
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
has terminal naked buds and axillary scaled buds that are in contrast with the description of 
<taxonomicName authorityName="G.Gardner &amp; Champion" authorityYear="1849" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma</emphasis>
</taxonomicName>
having naked-buds as given in the Flora of China (
<bibRefCitation author="Zhang, Z" journalOrPublisher="Flora of China" pageId="6" pageNumber="53" pagination="18 - 42" refId="B18" refString="Zhang, Z, Zhang, H, Endress, PK, 2003. Hamamelidaceae. Flora of China vol. 9: 18 - 42" title="Hamamelidaceae." volume="vol. 9" year="2003">Zhang et al. 2003</bibRefCitation>
). The naked bud is covered either with immature leaves that develop to foliage leaves or with their stipules, while the scaled bud is covered with cataphylls or stipules that are highly modified to protect the shoot tip (
<bibRefCitation author="Nitta, I" journalOrPublisher="Annals of Botany" pageId="6" pageNumber="53" pagination="115 - 129" publicationUrl="10.1006/anbo.1997.0545" refId="B11" refString="Nitta, I, Ohsawa, M, 1998. Bud structure and shoot architecture of canopy and understorey evergreen broad-leaved trees at their northern limit in east Asia. Annals of Botany 81: 115 - 129, DOI: 10.1006/anbo.1997.0545" title="Bud structure and shoot architecture of canopy and understorey evergreen broad-leaved trees at their northern limit in east Asia." url="10.1006/anbo.1997.0545" volume="81" year="1998">Nitta and Ohsawa 1998</bibRefCitation>
). The terminal buds of 
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
are classified as naked buds because they are incompletely covered by 2 stipules (Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4A, B, E &amp; F</figureCitation>
), while the axillary buds are classified as scaled buds because they are covered by 2 cataphylls that are subsequently shed or remained at the tip of previous shoot without further growth (Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4C, D, E &amp; F</figureCitation>
). The terminal bud of Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4B</figureCitation>
shows alternate leaf arrangement from the axillary bud, but same direction is also observed.
</paragraph>
<caption ID-Zenodo-Dep="974327" doi="10.3897/phytokeys.65.6726.figure2" httpUri="https://binary.pensoft.net/fig/92604" pageId="4" pageNumber="51" start="Figure 2" startId="F2">
<paragraph pageId="4" pageNumber="51">
<emphasis bold="true" pageId="4" pageNumber="51">Figure 2.</emphasis>
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
H.Toyama, Tagane &amp; V.S.Dang, sp. nov. 
<emphasis bold="true" pageId="4" pageNumber="51">A</emphasis>
capsule; 
<emphasis bold="true" pageId="4" pageNumber="51">B</emphasis>
immature fruits (lateral view, longitudinal and transverse sections from left) 
<emphasis bold="true" pageId="4" pageNumber="51">C</emphasis>
, axillary bud (ab), bud scale (bs), petiole (pt) and stipule (st) and uppermost branch (ub) 
<emphasis bold="true" pageId="4" pageNumber="51">D</emphasis>
bracteole 
<emphasis bold="true" pageId="4" pageNumber="51">E</emphasis>
fruiting branch 
<emphasis bold="true" pageId="4" pageNumber="51">F</emphasis>
vein with stellate hair 
<emphasis bold="true" pageId="4" pageNumber="51">G</emphasis>
young branch 
<emphasis bold="true" pageId="4" pageNumber="51">H</emphasis>
stellate hair on bud. [
<emphasis bold="true" pageId="4" pageNumber="51">A-H</emphasis>
from 
<emphasis italics="true" pageId="4" pageNumber="51">Tagane et al. V1586</emphasis>
, KYO. Drawn by H. Toyama.]
</paragraph>
</caption>
<caption ID-Zenodo-Dep="974329" doi="10.3897/phytokeys.65.6726.figure3" httpUri="https://binary.pensoft.net/fig/92605" pageId="4" pageNumber="51" start="Figure 3" startId="F3">
<paragraph pageId="4" pageNumber="51">
<emphasis bold="true" pageId="4" pageNumber="51">Figure 3.</emphasis>
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
H.Toyama, Tagane &amp; V.S.Dang, sp. nov. 
<emphasis bold="true" pageId="4" pageNumber="51">A</emphasis>
branch with infructescence 
<emphasis bold="true" pageId="4" pageNumber="51">B</emphasis>
fruits; 
<emphasis bold="true" pageId="4" pageNumber="51">C</emphasis>
abaxial surface of leaf 
<emphasis bold="true" pageId="4" pageNumber="51">D</emphasis>
young infructescence 
<emphasis bold="true" pageId="4" pageNumber="51">E</emphasis>
lamina showing glabrous abaxial surface 
<emphasis bold="true" pageId="4" pageNumber="51">F</emphasis>
seeds with a large hilum showing basal side on the placenta (upper), and apical side (lower). [
<emphasis bold="true" pageId="4" pageNumber="51">A-D</emphasis>
photographed on 14 July 2014, 
<emphasis bold="true" pageId="4" pageNumber="51">E</emphasis>
&amp; 
<emphasis bold="true" pageId="4" pageNumber="51">F</emphasis>
from 
<emphasis italics="true" pageId="4" pageNumber="51">Tagane et al. V1586</emphasis>
, KYO.]
</paragraph>
</caption>
<caption ID-Zenodo-Dep="974331" doi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51" start="Figure 4" startId="F4">
<paragraph pageId="4" pageNumber="51">
<emphasis bold="true" pageId="4" pageNumber="51">Figure 4.</emphasis>
Terminal and axillary bud of 
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
H.Toyama, Tagane &amp; V.S.Dang, sp. nov. 
<emphasis bold="true" pageId="4" pageNumber="51">A</emphasis>
terminal and axillary bud 
<emphasis bold="true" pageId="4" pageNumber="51">B</emphasis>
schematic diagram of transverse section of Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4A</figureCitation>
<emphasis bold="true" pageId="4" pageNumber="51">C</emphasis>
axillary bud 
<emphasis bold="true" pageId="4" pageNumber="51">D</emphasis>
schematic diagram of transverse section of Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4C</figureCitation>
<emphasis bold="true" pageId="4" pageNumber="51">E</emphasis>
elongated terminal and axillary shoot 
<emphasis bold="true" pageId="4" pageNumber="51">F</emphasis>
grayscale image of Fig. 
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Terminal and axillary bud of Eustigma honbaense H. Toyama, Tagane &amp; V. S. Dang, sp. nov. A terminal and axillary bud B schematic diagram of transverse section of Fig. 4 A C axillary bud D schematic diagram of transverse section of Fig. 4 C E elongated terminal and axillary shoot F grayscale image of Fig. 4 E. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [A &amp; C photographed on 22 November 2014, E &amp; F from Toyama et al. V 1975, FU.]" figureDoi="10.3897/phytokeys.65.6726.figure4" httpUri="https://binary.pensoft.net/fig/92606" pageId="4" pageNumber="51">4E</figureCitation>
. Abbreviations are as follows: axillary bud (ab), apical meristem (am), axillary shoot (as), bud scale (bs), branch (br), leaf (lf), petiole (pt), stipule (st), terminal shoot (ts). [
<emphasis bold="true" pageId="4" pageNumber="51">A</emphasis>
&amp; 
<emphasis bold="true" pageId="4" pageNumber="51">C</emphasis>
photographed on 22 November 2014, 
<emphasis bold="true" pageId="4" pageNumber="51">E</emphasis>
&amp; 
<emphasis bold="true" pageId="4" pageNumber="51">F</emphasis>
from 
<emphasis italics="true" pageId="4" pageNumber="51">Toyama et al. V1975</emphasis>
, FU.]
</paragraph>
</caption>
<paragraph pageId="4" pageNumber="51">
The description of bracts and bracteoles is insufficient because most of the bracts and bracteoles had been shed. 
<taxonomicName authorityName="Gardner &amp; Champ" authorityYear="1849" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma oblongifolium" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="oblongifolium">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma oblongifolium</emphasis>
</taxonomicName>
and 
<taxonomicName authorityName="C. Y. Wu" authorityYear="1977" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma lenticellatum" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="lenticellatum">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma lenticellatum</emphasis>
</taxonomicName>
have 3-bracteate flowers (
<bibRefCitation author="Gardner, G" journalOrPublisher="Hooker's Journal of Botany and Kew Garden Miscellany London" pageId="5" pageNumber="52" pagination="308 - 320" refId="B4" refString="Gardner, G, 1849. Descriptions of some new genera and species of plants, collected in the island of Hong Kong. Hooker's Journal of Botany and Kew Garden Miscellany London 1: 308 - 320" title="Descriptions of some new genera and species of plants, collected in the island of Hong Kong." volume="1" year="1849">Gardner 1849</bibRefCitation>
; 
<bibRefCitation author="Hsieh, CF" journalOrPublisher="Flora of Taiwan" pageId="6" pageNumber="53" pagination="1 - 9" refId="B7" refString="Hsieh, CF, 1993. Hamamelidaceae. Flora of Taiwan vol. 3: 1 - 9" title="Hamamelidaceae." volume="vol. 3" year="1993">Hsieh 1993</bibRefCitation>
; 
<bibRefCitation author="Wu, CY" journalOrPublisher="Flora Yunnanica" pageId="6" pageNumber="53" pagination="130 - 132" refId="B17" refString="Wu, CY, 1977. Eustigma lenticellatum C.Y.Wu. Flora Yunnanica vol. 1: 130 - 132" title="Eustigma lenticellatum C. Y. Wu." volume="vol. 1" year="1977">Wu 1977</bibRefCitation>
), while 
<taxonomicName authorityName="Oliv" authorityYear="1891" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma balansae" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="balansae">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma balansae</emphasis>
</taxonomicName>
has 2-bracteate flowers (
<bibRefCitation author="Oliver, D" journalOrPublisher="Hooker's Icones Plantarum; or Figures, with brief Descriptive Characters and Remarks of New or Rare Plants" pageId="6" pageNumber="53" refId="B12" refString="Oliver, D, 1891. Eustigma balansae Oliv. Hooker's Icones Plantarum; or Figures, with brief Descriptive Characters and Remarks of New or Rare Plants vol. 20" title="Eustigma balansae Oliv." volume="vol. 20" year="1891">Oliver 1891</bibRefCitation>
; 
<bibRefCitation author="Tardieu, ML" journalOrPublisher="Flore du Cambodge, du Laos et du Vietnam" pageId="6" pageNumber="53" pagination="106 - 107" refId="B16" refString="Tardieu, ML, 1965. Eustigma. Flore du Cambodge, du Laos et du Vietnam vol. 4: 106 - 107" title="Eustigma." volume="vol. 4" year="1965">Tardieu 1965</bibRefCitation>
). This could be a key trait to distinguish between species. Further collections of 
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
are needed.
</paragraph>
<paragraph pageId="4" pageNumber="51">
The 
<emphasis italics="true" pageId="4" pageNumber="51">matK</emphasis>
sequences between 
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
and 
<taxonomicName authorityName="Gardner &amp; Champ" authorityYear="1849" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma oblongifolium" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="oblongifolium">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma oblongifolium</emphasis>
</taxonomicName>
(AF013043) differ in 6 bases of the 781 total. The 
<emphasis italics="true" pageId="4" pageNumber="51">rbcL</emphasis>
and 
<emphasis italics="true" pageId="4" pageNumber="51">matK</emphasis>
sequences between 
<taxonomicName authorityName="H. Toyama, Tagane &amp; V. S. Dang" authorityYear="2016" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma honbaense" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="honbaense">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma honbaense</emphasis>
</taxonomicName>
and 
<taxonomicName authorityName="Oliv" authorityYear="1891" class="Magnoliopsida" family="Hamamelidaceae" genus="Eustigma" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Eustigma balansae" order="Saxifragales" pageId="4" pageNumber="51" phylum="Tracheophyta" rank="species" species="balansae">
<emphasis italics="true" pageId="4" pageNumber="51">Eustigma balansae</emphasis>
</taxonomicName>
(
<emphasis italics="true" pageId="4" pageNumber="51">rbcL</emphasis>
: HQ415214, 
<emphasis italics="true" pageId="4" pageNumber="51">matK</emphasis>
: HQ415379) differ in 1 base of the 517 total and 5 bases of the 761 total, respectively.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="51" type="genebank accession no">
<paragraph pageId="4" pageNumber="51">Genebank accession No.</paragraph>
<paragraph pageId="4" pageNumber="51">
<emphasis italics="true" pageId="4" pageNumber="51">Tagane et al. V1586</emphasis>
: LC005200 (
<emphasis italics="true" pageId="4" pageNumber="51">rbcL</emphasis>
), LC005201 (
<emphasis italics="true" pageId="4" pageNumber="51">matK</emphasis>
).
</paragraph>
</subSubSection>
</treatment>
</document>