231 lines
55 KiB
XML
231 lines
55 KiB
XML
<document id="91F57E018C3751F6752F255328E99F57" ID-DOI="10.1007/s13127-021-00514-y" ID-ISSN="1618-1077" ID-Zenodo-Dep="12764891" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1721247433783" checkinUser="felipe" docAuthor="Holzer, Victoria J. C., Kretschmann, Juliane, Knechtel, Johanna, Owsianny, Paweł M. & Gottschling, Marc" docDate="2022" docId="4D0999373815FFFFFCE8FAABD4746A3F" docLanguage="en" docName="OrgDivEvol.22.1.1-15.pdf" docOrigin="Organisms Diversity & Evolution 22 (1)" docSource="http://dx.doi.org/10.1007/s13127-021-00514-y" docStyle="DocumentStyle:92260E183F2ECC5FAA0643175D3F67A1.1:OrgDivEvol.2010-.journal_article.open" docStyleId="92260E183F2ECC5FAA0643175D3F67A1" docStyleName="OrgDivEvol.2010-.journal_article.open" docStyleVersion="1" docTitle="Peridinium volzii" docType="treatment" docVersion="3" lastPageNumber="5" masterDocId="B130E14F3817FFFBFFD8FFE3D0686E68" masterDocTitle="Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy" masterLastPageNumber="15" masterPageNumber="1" pageNumber="3" updateTime="1721741617348" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
|
||
<mods:mods id="6BF22E8F3DF32D5DC67FAD2EB520487D" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="E75F74C212BDCD34EBF7556AFA562811">
|
||
<mods:title id="5DFAE5C43CC9C5F0A2B48C739A117E72">Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="40AF93F2D8047F406AF4088926DA3B42" type="personal">
|
||
<mods:role id="63B2F1D6A06377906433EB960E02BEF9">
|
||
<mods:roleTerm id="8E71FCC9CFD1EEA69329DCDB7315B90B">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="D6AF2CA02BAA91EAF48ED8DD597F8575">Holzer, Victoria J. C.</mods:namePart>
|
||
<mods:affiliation id="1F733DEC9CDAB74865005450C0304298">Department Biologie: Systematics, Biodiversity & Evolution</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="CD904CA7292BADCA735E72EA424936C8" type="personal">
|
||
<mods:role id="64F1DD5BC746AD49A8747F4E672002EC">
|
||
<mods:roleTerm id="4AF963135272F8DDABDA858C0B178E11">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="5B9788AFD624D9E6F34EEDDD32268BD8">Kretschmann, Juliane</mods:namePart>
|
||
<mods:affiliation id="57DF046784D733D756FE39902409FE15">Department Biologie: Systematics, Biodiversity & Evolution</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="76540492F0D39326FA779061E9761812" type="personal">
|
||
<mods:role id="8623550188C4C3F87745A09C20CF7845">
|
||
<mods:roleTerm id="28043483ED602F7B1B5F70D4A822C370">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="9886725D706A78FE5AFC2608C98A4A72">Knechtel, Johanna</mods:namePart>
|
||
<mods:affiliation id="68ECD3D7F0D7163D4A736D7F6E298A4B">Department Biologie: Systematics, Biodiversity & Evolution</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="4CF1F21952EDE87212252DBC5B580418" type="personal">
|
||
<mods:role id="D50DB905068B2E12D3B15E40A4C420FF">
|
||
<mods:roleTerm id="86116FC469067F6064EB6A184D98945A">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="4043BC335348EA2E9A454D5089E5F497">Owsianny, Paweł M.</mods:namePart>
|
||
<mods:affiliation id="51406269D011AB6F97B4B1D930E7119A">Nadnotecki Institute UAM in Piła, Adam Mickiewicz</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="DD489A01C98B7784C440AC85B7E7E597" type="personal">
|
||
<mods:role id="85D0B2E2789ABC5D89BC5F057CC7DBB5">
|
||
<mods:roleTerm id="54756BA58398CAD3FF62B02C3E7A8DEC">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="FA9CCBEF05555EE1FB4B7FDA610309E9">Gottschling, Marc</mods:namePart>
|
||
<mods:affiliation id="676C8D3F28999098CBF9384E01B422FF">Department Biologie: Systematics, Biodiversity & Evolution</mods:affiliation>
|
||
</mods:name>
|
||
<mods:typeOfResource id="8EF21D139F8BD1F2087B62C2EFC05557">text</mods:typeOfResource>
|
||
<mods:relatedItem id="FE880B7401A03FE77AD0F18F546844F2" type="host">
|
||
<mods:titleInfo id="83D9492D0605376A2596464A354AF131">
|
||
<mods:title id="BD27F153A3AAD125CB37968202A54BF5">Organisms Diversity & Evolution</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="E29B79E05B5CA11D2A07361F128C0184">
|
||
<mods:date id="79C80F7A538CD9A7561E7A884169E4CB">2022</mods:date>
|
||
<mods:detail id="EDB290B878C6BB3F36D85E0CEF41A1AD" type="pubDate">
|
||
<mods:number id="36FA157888DB0D35333EC73438930D6E">2021-10-09</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="D9002F11CA57354185CF5BFF98DAAB70" type="volume">
|
||
<mods:number id="7A22167F34C366728561152D1BF8C2D4">22</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="4660CDCCF9B64A076E1A5A10E316AFAD" type="issue">
|
||
<mods:number id="1D13D255B1D87726DAB4499908BFF0DD">1</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="43835DBBEC3F08852B8BF98BDF4DD1C4" unit="page">
|
||
<mods:start id="F77B5B8681D2A16F8E2FE10B1BDF7563">1</mods:start>
|
||
<mods:end id="C63129DED9A03DC2356E5801BF717693">15</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location id="FD26F3DC3E483ACF971810F576DD476F">
|
||
<mods:url id="E3CAF4567FEE213831F2B75EB9C4F81F">http://dx.doi.org/10.1007/s13127-021-00514-y</mods:url>
|
||
</mods:location>
|
||
<mods:classification id="AE0D38206156DA2B7393689B27BC138F">journal article</mods:classification>
|
||
<mods:identifier id="4129872CBA1F24A5D32491FA823CCED9" type="DOI">10.1007/s13127-021-00514-y</mods:identifier>
|
||
<mods:identifier id="F547F413CB39519D1B43DA8F291BDE53" type="ISSN">1618-1077</mods:identifier>
|
||
<mods:identifier id="77D831CDFE0988312A7B23BD2EA4E194" type="Zenodo-Dep">12764891</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="4D0999373815FFFFFCE8FAABD4746A3F" LSID="urn:lsid:plazi:treatment:4D0999373815FFFFFCE8FAABD4746A3F" httpUri="http://treatment.plazi.org/id/4D0999373815FFFFFCE8FAABD4746A3F" lastPageId="4" lastPageNumber="5" pageId="2" pageNumber="3">
|
||
<subSubSection id="8DBA7BAA3815FFF9FCE8FAABD4D06B0B" box="[816,1208,1352,1379]" pageId="2" pageNumber="3" type="nomenclature">
|
||
<paragraph id="C51F28213815FFF9FCE8FAABD4D06B0B" blockId="2.[816,1208,1352,1379]" box="[816,1208,1352,1379]" pageId="2" pageNumber="3">
|
||
<heading id="9E579F4D3815FFF9FCE8FAABD4D06B0B" bold="true" box="[816,1208,1352,1379]" fontSize="11" level="1" pageId="2" pageNumber="3" reason="1">
|
||
<emphasis id="F7D4F4333815FFF9FCE8FAABD4D06B0B" bold="true" box="[816,1208,1352,1379]" pageId="2" pageNumber="3">
|
||
Morphology of
|
||
<taxonomicName id="02A053A23815FFF9FC33FAABD4D06B0B" authority="Lemmerm." box="[1003,1208,1352,1379]" class="Dinophyceae" family="Gymnodiniaceae" genus="Peridinium" kingdom="Chromista" order="Gymnodiniales" pageId="2" pageNumber="3" phylum="Miozoa" rank="genus">
|
||
<emphasis id="F7D4F4333815FFF9FC33FAABD4D06B0B" bold="true" box="[1003,1208,1352,1379]" italics="true" pageId="2" pageNumber="3">Peridinium. volzii</emphasis>
|
||
</taxonomicName>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="8DBA7BAA3815FFFFFCE8FA6FD4746A3F" lastPageId="4" lastPageNumber="5" pageId="2" pageNumber="3" type="description">
|
||
<paragraph id="C51F28213815FFF9FCE8FA6FD3A568C7" blockId="2.[816,1453,1420,1911]" pageId="2" pageNumber="3">
|
||
All investigated strains exhibited cells that were either flagellated (
|
||
<figureCitation id="5D9B34A43815FFF9FCA2FA4ED3A66BAD" box="[890,974,1453,1478]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="2" pageNumber="3">Fig. 1a, i</figureCitation>
|
||
) or coccoid (
|
||
<figureCitation id="5D9B34A43815FFF9FBB8FA4ED4AF6BAD" box="[1120,1223,1453,1478]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="2" pageNumber="3">Fig. 1k–m</figureCitation>
|
||
), with the motile cells being predominant. Motile cells swam homogenously within the medium or occasionally, they accumulated towards the light source. Each cell, except for necrotic cells, contained numerous gold- through olive-brownish chloroplasts and an orange to red accumulation body (
|
||
<figureCitation id="5D9B34A43815FFF9FB74F9B7D4856804" box="[1196,1261,1620,1644]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="2" pageNumber="3">Fig. 1i</figureCitation>
|
||
). An eyespot was absent. Necrotic cells included plastids that appeared grey through silver.
|
||
</paragraph>
|
||
<paragraph id="C51F28213815FFFFFC97F95BD2F96D23" blockId="2.[816,1453,1420,1911]" lastBlockId="4.[135,773,587,1844]" lastPageId="4" lastPageNumber="5" pageId="2" pageNumber="3">
|
||
All strains were indistinguishable from each other in gross morphology, and motile cells were continuously covered by a theca built of cellulosic plates (as inferred from astra blue staining). The thecate cells had an ovoid shape and were slightly compressed dorso-ventrally. The hypotheca was generally smaller than the epitheca. Average cell sizes of motile cells within the ten strains under detailed study ranged from 41 to 51 µm in length and from 36 to 48 µm in width (
|
||
<tableCitation id="88221D9A3813FFFFFF23FD6ED12A6CCD" box="[251,322,653,677]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Table 1</tableCitation>
|
||
). However, cell sizes of particular strains were significantly different from each other (
|
||
<figureCitation id="5D9B34A43813FFFFFDB7FD4CD2A56CAF" box="[623,717,687,711]" captionStart="Fig" captionStartId="5.[136,166,1784,1805]" captionTargetBox="[155,1432,1178,1733]" captionTargetId="figure-362@5.[151,1436,1174,1737]" captionTargetPageId="5" captionText="Fig. 2 Box plots displaying correlations between cell size (of motile cells; A, B) or side length (of empty thecate cells; C) in selected strains. Colours correspond to the ribotype of each strain (blue: ribotype I; magenta: ribotype II; orange: ribotype III). Statistically significant clusters are indicated with letters a, b and c and were calculated with Tukey’s Honest Significant Difference (HSD) test (p-values<0.05). Box plots depict percentile values from 25–75% (box), median (bar inside the box), standard deviation (whiskers) and outliers (dots)" figureDoi="http://doi.org/10.5281/zenodo.12764893" httpUri="https://zenodo.org/record/12764893/files/figure.png" pageId="4" pageNumber="5">Fig. 2a, b</figureCitation>
|
||
). All strains presented the thecal formula 4′, 3a, 7′′, 5c, 5s, 5′′′, 2′′′′, with few exceptions (see below). The surface of the plates was strongly reticulate, and each major plate of the
|
||
<typeStatus id="1A1B96833813FFFFFF51FCD7D0DB6D24" box="[137,179,820,844]" pageId="4" pageNumber="5" type="epitype">epi-</typeStatus>
|
||
and hypothecae contained one or two pores.
|
||
</paragraph>
|
||
<caption id="91DF78A93813FFFFFFA2FF72D1966C63" ID-DOI="http://doi.org/10.5281/zenodo.12800741" ID-Zenodo-Dep="12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5" startId="4.[122,136,145,168]" subCaptionStartIDs="4.[137,166,156,177]" subCaptionStarts="Fig.1 M" targetBox="[162,1428,158,1852]" targetPageId="3">
|
||
<paragraph id="C51F28213813FFFFFFA2FF72D1966C63" blockId="4.[122,773,145,527]" pageId="4" pageNumber="5">
|
||
<superScript id="32D585693813FFFFFFA2FF72D0E06EC0" attach="right" box="[122,136,145,168]" fontSize="8" pageId="4" pageNumber="5">◂</superScript>
|
||
<emphasis id="F7D4F4333813FFFFFF51FF7FD0D06ED9" bold="true" box="[137,184,156,177]" pageId="4" pageNumber="5">Fig.1</emphasis>
|
||
Morphology of thecate and coccoid cells, with labelled thecal plates.
|
||
<emphasis id="F7D4F4333813FFFFFF28FF56D1216EA2" bold="true" box="[240,329,181,203]" pageId="4" pageNumber="5">a–c, i, m</emphasis>
|
||
Light microscopy,
|
||
<emphasis id="F7D4F4333813FFFFFDDCFF56D2416EA2" bold="true" box="[516,553,181,202]" pageId="4" pageNumber="5">d–h</emphasis>
|
||
,
|
||
<emphasis id="F7D4F4333813FFFFFDE0FF56D23F6EA2" bold="true" box="[568,599,181,202]" pageId="4" pageNumber="5">k–l</emphasis>
|
||
scanning electron microscopy.
|
||
<emphasis id="F7D4F4333813FFFFFF27FF33D1626E8D" bold="true" box="[255,266,208,229]" pageId="4" pageNumber="5">a</emphasis>
|
||
Ventral view of strain GeoM*788;
|
||
<emphasis id="F7D4F4333813FFFFFDBCFF33D2196E8D" bold="true" box="[612,625,208,229]" pageId="4" pageNumber="5">b</emphasis>
|
||
dorsal view of strain GeoM*793;
|
||
<emphasis id="F7D4F4333813FFFFFEE0FF08D12A6F68" bold="true" box="[312,322,235,256]" pageId="4" pageNumber="5">c</emphasis>
|
||
apical view of strain GeoK*044;
|
||
<emphasis id="F7D4F4333813FFFFFDA7FF08D2E46F68" bold="true" box="[639,652,235,256]" pageId="4" pageNumber="5">d</emphasis>
|
||
ventral view of strain GeoK*037;
|
||
<emphasis id="F7D4F4333813FFFFFE80FEE6D10A6F72" bold="true" box="[344,354,261,282]" pageId="4" pageNumber="5">e</emphasis>
|
||
dorsal view of strain GeoM*788;
|
||
<emphasis id="F7D4F4333813FFFFFD62FEE6D2AA6F73" bold="true" box="[698,706,261,283]" pageId="4" pageNumber="5">f</emphasis>
|
||
apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line;
|
||
<emphasis id="F7D4F4333813FFFFFEB4FED8D11F6F38" bold="true" box="[364,375,315,336]" pageId="4" pageNumber="5">g</emphasis>
|
||
antapical view of strain GeoK*044;
|
||
<emphasis id="F7D4F4333813FFFFFD13FED8D2B06F38" bold="true" box="[715,728,315,336]" pageId="4" pageNumber="5">h</emphasis>
|
||
leftlateral view of strain GeoM*866;
|
||
<emphasis id="F7D4F4333813FFFFFE11FEB6D1A76F03" bold="true" box="[457,463,341,363]" pageId="4" pageNumber="5">i</emphasis>
|
||
motile cell of strain GeoK*037;
|
||
<emphasis id="F7D4F4333813FFFFFF50FE93D0DB6FED" bold="true" box="[136,179,368,389]" pageId="4" pageNumber="5">k–m</emphasis>
|
||
coccoid cells showing variability in shape and size of strains
|
||
<emphasis id="F7D4F4333813FFFFFD20FE93D36D6FED" bold="true" box="[760,773,368,389]" pageId="4" pageNumber="5">k</emphasis>
|
||
GeoM*866,
|
||
<emphasis id="F7D4F4333813FFFFFF25FE68D16B6FC8" bold="true" box="[253,259,395,416]" pageId="4" pageNumber="5">l</emphasis>
|
||
GeoM*793 and
|
||
<emphasis id="F7D4F4333813FFFFFE7DFE68D1D06FC8" bold="true" box="[421,440,395,416]" pageId="4" pageNumber="5">m</emphasis>
|
||
GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm.
|
||
<emphasis id="F7D4F4333813FFFFFE46FE15D1C66C63" box="[414,430,502,523]" italics="true" pageId="4" pageNumber="5">U</emphasis>
|
||
<subScript id="59242A643813FFFFFE76FDE2D1D16C67" attach="left" box="[430,441,513,527]" fontSize="6" pageId="4" pageNumber="5">A</subScript>
|
||
: 15 kV
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="C51F28213813FFFFFF7EFCB6D1356A95" blockId="4.[135,773,587,1844]" pageId="4" pageNumber="5">
|
||
The epithecal plate pattern was largely symmetrical, although the apical plate 2′ was always smaller than the apical plate 4′ (
|
||
<figureCitation id="5D9B34A43813FFFFFEC5FC7BD10D6DC7" box="[285,357,920,944]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1a</figureCitation>
|
||
, c-d, f). The apical plate 1′ appeared small, was rhomboid and ca 12–14 μm tall. Two significantly different suture lengths between the plates 1′ and 4′ were distinguishable across the strains (
|
||
<figureCitation id="5D9B34A43813FFFFFDCBFC1FD2316A7C" box="[531,601,1020,1044]" captionStart="Fig" captionStartId="5.[136,166,1784,1805]" captionTargetBox="[155,1432,1178,1733]" captionTargetId="figure-362@5.[151,1436,1174,1737]" captionTargetPageId="5" captionText="Fig. 2 Box plots displaying correlations between cell size (of motile cells; A, B) or side length (of empty thecate cells; C) in selected strains. Colours correspond to the ribotype of each strain (blue: ribotype I; magenta: ribotype II; orange: ribotype III). Statistically significant clusters are indicated with letters a, b and c and were calculated with Tukey’s Honest Significant Difference (HSD) test (p-values<0.05). Box plots depict percentile values from 25–75% (box), median (bar inside the box), standard deviation (whiskers) and outliers (dots)" figureDoi="http://doi.org/10.5281/zenodo.12764893" httpUri="https://zenodo.org/record/12764893/files/figure.png" pageId="4" pageNumber="5">Fig. 2c</figureCitation>
|
||
), being shorter (
|
||
<figureCitation id="5D9B34A43813FFFFFF43FBFED0B66A5D" box="[155,222,1053,1077]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1a</figureCitation>
|
||
) or longer (
|
||
<figureCitation id="5D9B34A43813FFFFFEB2FBFED1C96A5D" box="[362,417,1053,1077]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1</figureCitation>
|
||
c-d). Both apical plates 2′ and 4′ appeared hexagonal in shape and plate 3′ pentagonal. The apical plate 3′ is separated from plate 1′ by plates 2′ and 4′ and was either as wide as tall (
|
||
<figureCitation id="5D9B34A43813FFFFFE0BFB62D27A6AF1" box="[467,530,1153,1177]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1f</figureCitation>
|
||
) or slightly wider than tall (
|
||
<figureCitation id="5D9B34A43813FFFFFF19FB40D16C6AD3" box="[193,260,1187,1211]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1c</figureCitation>
|
||
). The intercalary plates 1a and 3a were pentagonal, had the same size and were smaller than the elongated plate 2a (
|
||
<figureCitation id="5D9B34A43813FFFFFF2DFB06D1386A95" box="[245,336,1253,1277]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1c, f</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="C51F28213813FFFFFF7EFAE4D1316823" blockId="4.[135,773,587,1844]" pageId="4" pageNumber="5">
|
||
Deviations from the archetypical plate pattern could be stated (
|
||
<tableCitation id="88221D9A3813FFFFFF0EFACBD1486B28" box="[214,288,1320,1344]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Tables 1</tableCitation>
|
||
,
|
||
<tableCitation id="88221D9A3813FFFFFEF3FACBD1506B28" box="[299,312,1320,1344]" captionStart="Table 2" captionStartId="6.[122,171,393,414]" captionTargetPageId="6" captionText="Table 2 Basionyms that have been associated with P. volzii, with diagnostic traits and further information as inferred from the protologues, and abundance of such traits in the investigated material" httpUri="http://table.plazi.org/id/91DF78A93811FFFDFFA2FE6AD0F16FD6" pageId="4" pageNumber="5" tableUuid="91DF78A93811FFFDFFA2FE6AD0F16FD6">2</tableCitation>
|
||
and
|
||
<tableCitation id="88221D9A3813FFFFFEBCFACBD1196B28" box="[356,369,1320,1344]" captionStart="Table3" captionStartId="8.[137,186,156,177]" captionTargetBox="[476,1450,165,372]" captionTargetPageId="8" captionText="Table3 Newly identified phenotypical variabilities in strains determined as P. volzii. Frequency determined for each ribotype (n = 100)" httpUri="http://table.plazi.org/id/91DF78A9381FFFF3FF51FF7FD1596F73" pageId="4" pageNumber="5" tableUuid="91DF78A9381FFFF3FF51FF7FD1596F73">3</tableCitation>
|
||
). Usually tetragonal, precingular plate 4′′ was occasionally pentagonal (
|
||
<figureCitation id="5D9B34A43813FFFFFE0FFAAAD2506B09" box="[471,568,1353,1377]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">Fig. 3a, b</figureCitation>
|
||
) and abutted plate 3a, from which it was otherwise separated. This conformation affected the position and size of the intercalary plate 2a, which appeared smaller and less elongated. In few individual cells of all strain, the intercalary plate 2a (
|
||
<figureCitation id="5D9B34A43813FFFFFD90FA2CD2E26B8F" box="[584,650,1487,1511]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">Fig. 3c</figureCitation>
|
||
), the apical plate 3′ (
|
||
<figureCitation id="5D9B34A43813FFFFFF35FA13D1506860" box="[237,312,1520,1544]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">
|
||
Fig.
|
||
<quantity id="025885C43813FFFFFECDFA13D1506860" box="[277,312,1520,1544]" metricMagnitude="0" metricUnit="m" metricValue="3.0" pageId="4" pageNumber="5" unit="m" value="3.0">3m</quantity>
|
||
</figureCitation>
|
||
) or the postcingular plate 3′′′ (
|
||
<figureCitation id="5D9B34A43813FFFFFD5CFA13D2A96860" box="[644,705,1520,1544]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">Fig. 3i</figureCitation>
|
||
) were split, and also unusual fusions of plates could be observed (
|
||
<tableCitation id="88221D9A3813FFFFFF40F9D0D0B66823" box="[152,222,1587,1611]" captionStart="Table3" captionStartId="8.[137,186,156,177]" captionTargetBox="[476,1450,165,372]" captionTargetPageId="8" captionText="Table3 Newly identified phenotypical variabilities in strains determined as P. volzii. Frequency determined for each ribotype (n = 100)" httpUri="http://table.plazi.org/id/91DF78A9381FFFF3FF51FF7FD1596F73" pageId="4" pageNumber="5" tableUuid="91DF78A9381FFFF3FF51FF7FD1596F73">Table 3</tableCitation>
|
||
;
|
||
<figureCitation id="5D9B34A43813FFFFFF33F9D0D1256823" box="[235,333,1587,1611]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">Fig. 3d, f</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="C51F28213813FFFFFF7EF9B7D5006EBA" blockId="4.[135,773,587,1844]" lastBlockId="4.[816,1454,153,1111]" pageId="4" pageNumber="5">
|
||
The cingulum was median or sub-median and descending. The displacement was significantly different between the strains (
|
||
<tableCitation id="88221D9A3813FFFFFF3EF974D14668C7" box="[230,302,1687,1711]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Table 1</tableCitation>
|
||
), with either ca 1 cingulum width (
|
||
<figureCitation id="5D9B34A43813FFFFFD60F974D29368C7" box="[696,763,1687,1711]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1a</figureCitation>
|
||
) or ca 1.5–2 cingulum widths (
|
||
<figureCitation id="5D9B34A43813FFFFFE16F95BD27C68B8" box="[462,532,1720,1744]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1d</figureCitation>
|
||
). Notably, this distinction correlated with the two different suture lengths between apical plates 1′ and 4′ (
|
||
<tableCitation id="88221D9A3813FFFFFE58F918D1AE697B" box="[384,454,1787,1811]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Table 1</tableCitation>
|
||
). The sulcus appeared narrow and extended into the epitheca for approximately a cingulum width (
|
||
<figureCitation id="5D9B34A43813FFFFFC5CFF7AD38C6ED9" box="[900,996,153,178]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1a, d</figureCitation>
|
||
). Two flagella originated from the junction between sulcus and cingulum and were visible in LM.
|
||
</paragraph>
|
||
<paragraph id="C51F28213813FFFFFC97FF3FD5216D62" blockId="4.[816,1454,153,1111]" pageId="4" pageNumber="5">
|
||
Cell division was by eleutheroschisis. The resulting empty thecae were collected in high quantity at the bottom of the cultivation plate. The theca opened along the edge of the cingulum that epitheca and hypotheca were separated. The predominant opening of the epitheca was exhibited at the apical side between the
|
||
<specimenCount id="D3A6E3A83813FFFFFBF2FE60D4BD6FF3" box="[1066,1237,387,411]" count="3" pageId="4" pageNumber="5" type="intercalary">three intercalary</specimenCount>
|
||
plates and the plates 2′ and 4′ (
|
||
<figureCitation id="5D9B34A43813FFFFFC7AFE47D38B6FD4" box="[930,995,420,444]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1f</figureCitation>
|
||
). The cell opening continued at the sutures between the precingular plates 5′′ and 6′′ as well as 2′′ and 3′′, resulting in the release of the dorsal part of the epitheca. Subsequently, this thecal split led to the release of the ventral epitheca, as well as the separation of epitheca and hypotheca (
|
||
<figureCitation id="5D9B34A43813FFFFFC99FDA8D3FB6C0B" box="[833,915,587,611]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">
|
||
Fig.
|
||
<quantity id="025885C43813FFFFFCB5FDA8D3FB6C0B" box="[877,915,587,611]" metricMagnitude="0" metricUnit="m" metricValue="1.0" pageId="4" pageNumber="5" unit="m" value="1.0">1m</quantity>
|
||
</figureCitation>
|
||
). This specific dehiscence line was present in all strains with a frequency of 66–82% (
|
||
<tableCitation id="88221D9A3813FFFFFB0CFD8FD5756CEC" box="[1236,1309,620,644]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Table 1</tableCitation>
|
||
). Deviations from the regular opening line predominantly occurred at the precingular plates in all strains investigated. Sutures between thecal plates varied from thin lines through wide bands, the latter exhibiting cross striations (
|
||
<figureCitation id="5D9B34A43813FFFFFB4FFD12D4896D61" box="[1175,1249,753,778]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Figs. 1f</figureCitation>
|
||
,
|
||
<figureCitation id="5D9B34A43813FFFFFB34FD11D5546D62" box="[1260,1340,753,778]" captionStart="◂" captionStartId="10.[122,136,145,168]" captionTargetId="figure-6@9.[160,1427,154,1854]" captionTargetPageId="9" captionText="◂Fig.3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar=10 µm. U A=15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800743" httpUri="https://zenodo.org/record/12800743/files/figure.png" pageId="4" pageNumber="5">3b, e, g</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="C51F28213813FFFFFC97FCF0D4746A3F" blockId="4.[816,1454,153,1111]" pageId="4" pageNumber="5">
|
||
Coccoid cells varied in their average size from 39 to 50 µm in length and from 32 to 45 µm in width (
|
||
<tableCitation id="88221D9A3813FFFFFAE8FCD7D51E6D24" box="[1328,1398,820,844]" captionStart="Table1" captionStartId="5.[137,186,156,177]" captionTargetPageId="5" captionText="Table1 Size of motile and coccoid cells, length of specific sutures and frequency of morphological characteristics including thecal opening of seven selected strains" httpUri="http://table.plazi.org/id/91DF78A93812FFFEFF51FF7FD1226EA3" pageId="4" pageNumber="5" tableUuid="91DF78A93812FFFEFF51FF7FD1226EA3">Table 1</tableCitation>
|
||
) and were either thecate or athecate. Their shapes were variable ranging from spherical through obovoid. Some coccoid cells exhibited a slight dorso-ventrally flattening (
|
||
<figureCitation id="5D9B34A43813FFFFFAD7FC7BD5266DD8" box="[1295,1358,920,944]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">Fig. 1l</figureCitation>
|
||
). Within these cells, brown granules of varying sizes were observed in addition to the plate-like chloroplasts. A single coccoid cell was observed to develop intrathecately (
|
||
<figureCitation id="5D9B34A43813FFFFFAF9FC1FD5186A7C" box="[1313,1392,1020,1044]" captionStart="◂" captionStartId="4.[122,136,145,168]" captionTargetId="figure-7@3.[160,1428,154,1854]" captionTargetPageId="3" captionText="◂Fig.1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV" figureDoi="http://doi.org/10.5281/zenodo.12800741" httpUri="https://zenodo.org/record/12800741/files/figure.png" pageId="4" pageNumber="5">
|
||
Fig.
|
||
<quantity id="025885C43813FFFFFA93FC1FD5186A7C" box="[1355,1392,1020,1044]" metricMagnitude="0" metricUnit="m" metricValue="1.0" pageId="4" pageNumber="5" unit="m" value="1.0">1m</quantity>
|
||
</figureCitation>
|
||
), and the ecdysing cell was released by thecal opening along the edge of the cingulum.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |