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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.67.51020" ID-GBIF-Dataset="ab7b0e2e-b871-472e-a7d3-38210c30b1ea" ID-PMC="PMC7242484" ID-Pensoft-Pub="1314-4049-67-33" ID-Pensoft-UUID="E906233487A756EA8713B392155401B8" ID-PubMed="32476981" ModsDocID="1314-4049-67-33" checkinTime="1589507785129" checkinUser="pensoft" docAuthor="Liu, Hai-Ying, Li, Yan-Chun &amp; Bau, Tolgor" docDate="2020" docId="E4227468BD4D562F9F6D8E71FFF13E81" docLanguage="en" docName="MycoKeys 67: 33-44" docOrigin="MycoKeys 67" docSource="http://dx.doi.org/10.3897/mycokeys.67.51020" docTitle="Retiboletus sinogriseus Yan C. Li &amp; T. Bau 2020, sp. nov." docType="treatment" docVersion="4" id="E906233487A756EA8713B392155401B8" lastPageNumber="33" masterDocId="E906233487A756EA8713B392155401B8" masterDocTitle="New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data" masterLastPageNumber="44" masterPageNumber="33" pageNumber="33" updateTime="1668136454491" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Liu, Hai-Ying</mods:namePart>
<mods:affiliation>Institute of Mycology, Jilin Agricultural University, Changchun 130118, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Li, Yan-Chun</mods:namePart>
<mods:affiliation>Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, 650201, Kunming, China</mods:affiliation>
<mods:nameIdentifier type="email">liyanch@mail.kib.ac.cn</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Bau, Tolgor</mods:namePart>
<mods:affiliation>Institute of Mycology, Jilin Agricultural University, Changchun 130118, China</mods:affiliation>
<mods:nameIdentifier type="email">junwusuo@126.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2020</mods:date>
<mods:detail type="volume">
<mods:number>67</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>33</mods:start>
<mods:end>44</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.67.51020</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.67.51020</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-67-33</mods:identifier>
<mods:identifier type="Pensoft-UUID">E906233487A756EA8713B392155401B8</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="164158513" LSID="urn:lsid:plazi:treatment:E4227468BD4D562F9F6D8E71FFF13E81" httpUri="http://treatment.plazi.org/id/E4227468BD4D562F9F6D8E71FFF13E81" lastPageNumber="33" pageId="0" pageNumber="33">
<subSubSection pageId="0" pageNumber="33" type="nomenclature">
<paragraph pageId="0" pageNumber="33">
<taxonomicName LSID="E4227468-BD4D-562F-9F6D-8E71FFF13E81" authority="Yan C. Li &amp; T. Bau" authorityName="Yan C. Li &amp; T. Bau" authorityYear="2020" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus sinogriseus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="sinogriseus" status="sp. nov.">Retiboletus sinogriseus Yan C. Li &amp; T. Bau</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="33">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Habitat of the new Retiboletus species. a-c R. ater (from KUN-HKAS 56069) d-f R. sinogriseus (d from KUN-HKAS 91288 e-f from KUN-HKAS 91286)." figureDoi="10.3897/mycokeys.67.51020.figure2" httpUri="https://binary.pensoft.net/fig/409844" pageId="0" pageNumber="33">Figures 2d-f</figureCitation>
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Microscopic features of R. sinogriseus (KUN-HKAS 912889). a Basidiospores b basidia and pleurocystidium c pleurocystidia d cheilocystidia e pileipellis. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.67.51020.figure4" httpUri="https://binary.pensoft.net/fig/409846" pageId="0" pageNumber="33">, 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="etymology">
<paragraph pageId="0" pageNumber="33">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="33">
<emphasis italics="true" pageId="0" pageNumber="33">sino</emphasis>
(Latin) = China, reflecting that the basidiomata were collected in China +
<taxonomicName authorityName="Manfr. Binder &amp; Bresinsky" authorityYear="2002" baseAuthorityName="Frost" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="griseus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">griseus</emphasis>
</taxonomicName>
for similarity of the basidiomata of this species to
<taxonomicName authorityName="Manfr. Binder &amp; Bresinsky" authorityYear="2002" baseAuthorityName="Frost" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus griseus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">Retiboletus griseus</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="materials_examined">
<paragraph pageId="0" pageNumber="33">Type.</paragraph>
<paragraph pageId="0" pageNumber="33">China. Liaoning Province: Anshan City, Qianshan, alt. 400 m, 25 Aug 2015, J. Li 260 (holotype: KUN-HKAS 91288!).</paragraph>
<caption doi="10.3897/mycokeys.67.51020.figure4" httpUri="https://binary.pensoft.net/fig/409846" pageId="0" pageNumber="33" start="Figure 4" startId="F4">
<paragraph pageId="0" pageNumber="33">
<emphasis bold="true" pageId="0" pageNumber="33">Figure 4.</emphasis>
Microscopic features of
<taxonomicName lsidName="R. sinogriseus" pageId="0" pageNumber="33" rank="species" species="sinogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. sinogriseus</emphasis>
</taxonomicName>
(KUN-HKAS 912889).
<emphasis bold="true" pageId="0" pageNumber="33">a</emphasis>
Basidiospores
<emphasis bold="true" pageId="0" pageNumber="33">b</emphasis>
basidia and pleurocystidium
<emphasis bold="true" pageId="0" pageNumber="33">c</emphasis>
pleurocystidia
<emphasis bold="true" pageId="0" pageNumber="33">d</emphasis>
cheilocystidia
<emphasis bold="true" pageId="0" pageNumber="33">e</emphasis>
pileipellis. Scale bars: 10
<normalizedToken originalValue="μm">μm</normalizedToken>
.
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="description">
<paragraph pageId="0" pageNumber="33">Description.</paragraph>
<paragraph pageId="0" pageNumber="33">
Basidiomata medium-sized. Pileus 6-7.2 cm in diameter, subhemispherical to applanate, sometimes convex; surface tomentose, grayish-brown (5D2-3) to brown (5E4), rimose when dry, always cracked into small squamules on grayish (4B1) to whitish (2A1) background; context 1-2 cm thick in the center of pileus, white (1A1), unchanging when injured. Hymenophore adnate, sometimes slightly depressed around apex of stipe; pores angular, 0.3-1 mm wide, tubes up to 14 mm long, yellow (4C3-4) to grayish-yellow (4B2-3), unchanging when injured. Stipe 6-8
<normalizedToken originalValue="×">x</normalizedToken>
1.1-1.5 cm, subcylindrical, solid; surface dry, pale yellow at apex, blackish-yellow towards the base, entirely covered with moderately developed reticulum; context white to cream in the upper part and yellowish to yellow downwards, unchanging when injured; basal mycelium yellow. Taste and odor indistinct.
</paragraph>
<paragraph pageId="0" pageNumber="33">
Basidia 21-27
<normalizedToken originalValue="×">x</normalizedToken>
9-11
<normalizedToken originalValue="μm">μm</normalizedToken>
, clavate, thin-walled, four-spored; sterigmata 4-5
<normalizedToken originalValue="μm">μm</normalizedToken>
long. Basidiospores [40/2/2] (9) 10.0-13.5 (-14.0)
<normalizedToken originalValue="×">x</normalizedToken>
(3) 4.0-5.0 (-5.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
, Q = (2.25-) 2.5-3.25 (-3.42), Qm = 2.88
<normalizedToken originalValue="±">+/-</normalizedToken>
0.32, subfusiform to ellipsoid, slightly thick-walled (up to 0.5
<normalizedToken originalValue="μm">μm</normalizedToken>
), hyaline to yellowish in KOH, olive-brown to yellowish-brown in
<normalizedToken originalValue="Melzers">Melzer's</normalizedToken>
reagent, smooth. Hymenophoral trama boletoid. Cheilo- and pleurocystidia 35-56
<normalizedToken originalValue="×">x</normalizedToken>
7-12
<normalizedToken originalValue="μm">μm</normalizedToken>
, abundant, subfusiform to fusiform, thin-walled, with yellowish-brown to brown contents, without encrustations. Pileipellis a subcutis, 100-120
<normalizedToken originalValue="μm">μm</normalizedToken>
thick, composed of thin-walled filamentous hyphae 4-7
<normalizedToken originalValue="μm">μm</normalizedToken>
wide, with subcylindrical to clavate terminal cells 33-72
<normalizedToken originalValue="×">x</normalizedToken>
4-6
<normalizedToken originalValue="μm">μm</normalizedToken>
, sometimes with subacute apex, colorless to pale yellowish-brown in KOH, yellow-brown to brownish in
<normalizedToken originalValue="Melzers">Melzer's</normalizedToken>
reagent. Pileal trama composed of thin-walled hyphae 4-9
<normalizedToken originalValue="μm">μm</normalizedToken>
wide, colorless to pale yellowish-brown in KOH, yellow-brown to brownish in
<normalizedToken originalValue="Melzers">Melzer's</normalizedToken>
reagent. Clamp connections absent in all tissues.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="habitat">
<paragraph pageId="0" pageNumber="33">Habitat, ecology and distribution.</paragraph>
<paragraph pageId="0" pageNumber="33">
Solitary on the ground in mixed forests dominated by plants in the families
<taxonomicName family="Fagaceae" lsidName="" pageId="0" pageNumber="33" rank="family">Fagaceae</taxonomicName>
and
<taxonomicName family="Pinaceae" lsidName="" pageId="0" pageNumber="33" rank="family">Pinaceae</taxonomicName>
; currently known from northeastern China.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="materials_examined">
<paragraph pageId="0" pageNumber="33">Additional specimens examined.</paragraph>
<paragraph pageId="0" pageNumber="33">China. Liaoning Province: Anshan City, Qianshan, alt. 400 m, 25 Aug 2015, J. Li 258 (KUN-HKAS 91286).</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="33" type="discussion">
<paragraph pageId="0" pageNumber="33">Discussion.</paragraph>
<paragraph pageId="0" pageNumber="33">
<taxonomicName authorityName="Yan C. Li &amp; T. Bau" authorityYear="2020" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus sinogriseus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="sinogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">Retiboletus sinogriseus</emphasis>
</taxonomicName>
has a grayish-brown to brown pileus, a pale yellow to blackish-yellow stipe. Such traits are very similar to those of
<taxonomicName lsidName="R. griseus" pageId="0" pageNumber="33" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. griseus</emphasis>
</taxonomicName>
. Interestingly,
<taxonomicName lsidName="R. sinogriseus" pageId="0" pageNumber="33" rank="species" species="sinogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. sinogriseus</emphasis>
</taxonomicName>
clusters with
<taxonomicName lsidName="R. griseus" pageId="0" pageNumber="33" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. griseus</emphasis>
</taxonomicName>
with strong statistical support (Fig.
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Maximum likelihood phylogenetic tree of Retiboletus inferred from the combined nuclear dataset (nrLSU + TEF 1 - α). Bootstrap frequencies (&gt; 50 %) are shown above supported branches. Newly sequenced collections are boldfaced in black. Species vouchers are provided after the species name." figureDoi="10.3897/mycokeys.67.51020.figure1" httpUri="https://binary.pensoft.net/fig/409843" pageId="0" pageNumber="33">1</figureCitation>
). However,
<taxonomicName lsidName="R. griseus" pageId="0" pageNumber="33" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. griseus</emphasis>
</taxonomicName>
, originally described from North America but not found in China yet, has a distinctly pallid hymenohore and broad pileipellis hyphae which are up to 17
<normalizedToken originalValue="μm">μm</normalizedToken>
wide (
<bibRefCitation DOI="https://doi.org/10.2307/2421647" author="Singer, R" journalOrPublisher="American Midland Naturalist" pageId="0" pageNumber="33" pagination="1 - 135" refId="B8" refString="Singer, R, 1947. The Boletoideae of Florida with notes on extralimital species III. American Midland Naturalist 37: 1 - 135, DOI: https://doi.org/10.2307/2421647" title="The Boletoideae of Florida with notes on extralimital species III." url="https://doi.org/10.2307/2421647" volume="37" year="1947">Singer 1947</bibRefCitation>
;
<bibRefCitation author="Smith, AH" journalOrPublisher="American Midland Naturalist" pageId="0" pageNumber="33" refId="B9" refString="Smith, AH, Thiers, HD, 1971. The boletes of Michigan. University of Michigan Press, Ann Arbor, 1-428." title="The boletes of Michigan. University of Michigan Press, Ann Arbor, 1 - 428." year="1971">Smith and Thiers 1971</bibRefCitation>
;
<bibRefCitation author="Ortiz-Santana, B" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="33" pagination="247 - 416" refId="B7" refString="Ortiz-Santana, B, Lodge, DJ, Baroni, TJ, Both, EE, 2007. Boletes from Belize and the Dominican Republic. Fungal Diversity 27: 247 - 416" title="Boletes from Belize and the Dominican Republic." volume="27" year="2007">Ortiz-Santana et al. 2007</bibRefCitation>
). Additionally, the
<taxonomicName lsidName="R. sinogriseus" pageId="0" pageNumber="33" rank="species" species="sinogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. sinogriseus</emphasis>
</taxonomicName>
/
<taxonomicName lsidName="R. griseus" pageId="0" pageNumber="33" rank="species" species="griseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. griseus</emphasis>
</taxonomicName>
clade is clustered with
<taxonomicName lsidName="R. zhangfeii" pageId="0" pageNumber="33" rank="species" species="zhangfeii">
<emphasis italics="true" pageId="0" pageNumber="33">R. zhangfeii</emphasis>
</taxonomicName>
N.K. Zeng &amp; Zhu L. Yang,
<taxonomicName lsidName="R. nigrogriseus" pageId="0" pageNumber="33" rank="species" species="nigrogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. nigrogriseus</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="R. nigerrimus" pageId="0" pageNumber="33" rank="species" species="nigerrimus">
<emphasis italics="true" pageId="0" pageNumber="33">R. nigerrimus</emphasis>
</taxonomicName>
(
<taxonomicName genus="R." lsidName="R." pageId="0" pageNumber="33" rank="genus">R.</taxonomicName>
Heim) Manfr. Binder &amp; Bresinsky (however without bootstrap support). In this assemblage,
<taxonomicName lsidName="R. zhangfeii" pageId="0" pageNumber="33" rank="species" species="zhangfeii">
<emphasis italics="true" pageId="0" pageNumber="33">R. zhangfeii</emphasis>
</taxonomicName>
differs significantly from
<taxonomicName lsidName="R. sinogriseus" pageId="0" pageNumber="33" rank="species" species="sinogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">R. sinogriseus</emphasis>
</taxonomicName>
by its differently colored pileus, hymenophore, stipe and context (
<bibRefCitation DOI="https://doi.org/10.3852/15-072" author="Zeng, NK" journalOrPublisher="Mycologia" pageId="0" pageNumber="33" pagination="363 - 380" refId="B14" refString="Zeng, NK, Liang, ZQ, Wu, G, Li, YC, Yang, ZL, Liang, ZQ, 2016. The genus Retiboletus in China. Mycologia 108: 363 - 380, DOI: https://doi.org/10.3852/15-072" title="The genus Retiboletus in China." url="https://doi.org/10.3852/15-072" volume="108" year="2016">Zeng et al. 2016</bibRefCitation>
).
<taxonomicName authorityName="N.K.Zeng, S.Jiang &amp; Zhi Q.Liang" authorityYear="2018" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus nigrogriseus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="nigrogriseus">
<emphasis italics="true" pageId="0" pageNumber="33">Retiboletus nigrogriseus</emphasis>
</taxonomicName>
has a black to gray pileus, white to grayish white hymenophore, white to olivaceous contex in the stipe and much smaller basidiospores 8-10.5
<normalizedToken originalValue="×">x</normalizedToken>
3.5-4.5
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<taxonomicName authorityName="Manfr. Binder &amp; Bresinsky" authorityYear="2002" baseAuthorityName="R. Heim" class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus nigerrimus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="species" species="nigerrimus">
<emphasis italics="true" pageId="0" pageNumber="33">Retiboletus nigerrimus</emphasis>
</taxonomicName>
, originally described from Papua New Guinea, has a pileus with a distinctive blue tinge, a context lemon yellow in pileus and orange in the base of stipe and longer and narrower basidiospores 11.5-14.5
<normalizedToken originalValue="×">x</normalizedToken>
3.6-4.6
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<bibRefCitation author="Heim, R" journalOrPublisher="Revue mycologique" pageId="0" pageNumber="33" pagination="277 - 283" refId="B5" refString="Heim, R, 1963. Diagnoses latines des especes de champignons, ou nonda associes a la folie du komugl tai et dundaal. Revue mycologique 28: 277 - 283" title="Diagnoses latines des especes de champignons, ou nonda associes a la folie du komugl tai et dundaal." volume="28" year="1963">Heim 1963</bibRefCitation>
).
</paragraph>
<paragraph pageId="0" pageNumber="33">
Nine species of
<taxonomicName class="Agaricomycetes" family="Boletaceae" genus="Retiboletus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Retiboletus" order="Boletales" pageId="0" pageNumber="33" phylum="Basidiomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="33">Retiboletus</emphasis>
</taxonomicName>
were recorded from China, including two new species described herein. For the convenience of identification, a key to the species in China is given below.
</paragraph>
</subSubSection>
</treatment>
</document>