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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.57.35448" ID-GBIF-Dataset="bf107fa5-ec57-420f-bb6d-8c2853845429" ID-PMC="PMC6717119" ID-Pensoft-Pub="1314-4049-57-113" ID-Pensoft-UUID="5EEB9835D93A5DD7B2C25ABC5216FD7B" ID-PubMed="31523165" ModsDocID="1314-4049-57-113" checkinTime="1566623487220" checkinUser="pensoft" docAuthor="Long, Hui, Zhang, Qian, Hao, Yuan-Yuan, Shao, Xian-Qiang, Wei, Xiao-Xing, Hyde, Kevin D., Wang, Yong &amp; Zhao, De-Gang" docDate="2019" docId="AB53D9B53F1B5833915C42D14348045C" docLanguage="en" docName="MycoKeys 57: 113-127" docOrigin="MycoKeys 57" docSource="http://dx.doi.org/10.3897/mycokeys.57.35448" docTitle="Diaporthe osmanthi H. Long, K. D. Hyde &amp; Yong Wang bis, sp. nov." docType="treatment" docVersion="4" id="5EEB9835D93A5DD7B2C25ABC5216FD7B" lastPageNumber="113" masterDocId="5EEB9835D93A5DD7B2C25ABC5216FD7B" masterDocTitle="Diaporthe species in south-western China" masterLastPageNumber="127" masterPageNumber="113" pageNumber="113" updateTime="1668136291731" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Diaporthe species in south-western China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Long, Hui</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhang, Qian</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hao, Yuan-Yuan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Shao, Xian-Qiang</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wei, Xiao-Xing</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hyde, Kevin D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Yong</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhao, De-Gang</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2019</mods:date>
<mods:detail type="volume">
<mods:number>57</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>113</mods:start>
<mods:end>127</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.57.35448</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.57.35448</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-57-113</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="159244656" LSID="urn:lsid:plazi:treatment:AB53D9B53F1B5833915C42D14348045C" httpUri="http://treatment.plazi.org/id/AB53D9B53F1B5833915C42D14348045C" lastPageNumber="113" pageId="0" pageNumber="113">
<subSubSection pageId="0" pageNumber="113" type="nomenclature">
<paragraph pageId="0" pageNumber="113">
<taxonomicName LSID="ab53d9b5-3f1b-5833-915c-42d14348045c" authority="H. Long, K. D. Hyde &amp; Yong Wang bis" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">Diaporthe osmanthi H. Long, K.D. Hyde &amp; Yong Wang bis</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="113">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Diaporthe osmanthi (GUCC 9165). a-b upper (a) and lower (b) surface of colony on PDA c-d conidiomata e conidiophores, conidiogenous loci and conidia f α-conidia g two types of conidia h β-conidia. Scale bars: 10 µm (e, f, g, h)." figureDoi="10.3897/mycokeys.57.35448.figure4" httpUri="https://binary.pensoft.net/fig/328114" pageId="0" pageNumber="113">Figure 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="diagnosis">
<paragraph pageId="0" pageNumber="113">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="113">
Characterised by size of
<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
and
<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="materials_examined">
<paragraph pageId="0" pageNumber="113">Type.</paragraph>
<paragraph pageId="0" pageNumber="113">
China, Guangxi province, Nanning City, from leaves of
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Osmanthus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Osmanthus fragrans" order="Lamiales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="fragrans">
<emphasis italics="true" pageId="0" pageNumber="113">Osmanthus fragrans</emphasis>
</taxonomicName>
, 20 September, 2016, Y. Wang, HGUP 9165, holotype, ex-type living culture GUCC 9165.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="description">
<paragraph pageId="0" pageNumber="113">Description.</paragraph>
<paragraph pageId="0" pageNumber="113">
<emphasis italics="true" pageId="0" pageNumber="113">Colonies</emphasis>
on PDA attaining 9 cm diam. after 10 days; coralloid with feathery branches at margin, adpressed, without aerial mycelium, with numerous irregularly zonated dark stromata, isabelline becoming lighter towards the margin; reverse similar to the surface with zonations more apparent.
<emphasis italics="true" pageId="0" pageNumber="113">Conidiomata</emphasis>
pycnidial and multilocular, scattered, abundant on PDA after 3 wks, globose, subglobose or irregular, up to 1-1.5 mm diam., ostiolate, necks absent or up to 1 mm.
<emphasis italics="true" pageId="0" pageNumber="113">Conidiophores</emphasis>
formed from the inner layer of the locular wall, reduced to conidiogenous cells or 1-septate, hyaline to pale yellowish-brown, cylindrical, 20.5-61
<normalizedToken originalValue="×">x</normalizedToken>
1-3
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="113">Conidiogenous cells</emphasis>
cylindrical to flexuous, tapered towards apex, hyaline, 10-15
<normalizedToken originalValue="×">x</normalizedToken>
1.5-3
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="113">Alpha conidia</emphasis>
abundant, fusiform, narrowed towards the apex and base, apparently biguttulate, hyaline, 5.5-8.5
<normalizedToken originalValue="×">x</normalizedToken>
2-3
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="113">Beta conidia</emphasis>
scarce to abundant, flexuous to J-shaped, hyaline, 20-31.5
<normalizedToken originalValue="×">x</normalizedToken>
1-2.5
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="113">Perithecia</emphasis>
not seen.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="habitat">
<paragraph pageId="0" pageNumber="113">Habitat and distribution.</paragraph>
<paragraph pageId="0" pageNumber="113">
Isolated from leaves of
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Osmanthus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Osmanthus fragrans" order="Lamiales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="fragrans">
<emphasis italics="true" pageId="0" pageNumber="113">Osmanthus fragrans</emphasis>
</taxonomicName>
in China.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="etymology">
<paragraph pageId="0" pageNumber="113">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="113">
Species epithet
<taxonomicName lsidName="osmanthi" pageId="0" pageNumber="113" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">osmanthi</emphasis>
</taxonomicName>
, referring to the host,
<taxonomicName class="Magnoliopsida" family="Oleaceae" genus="Osmanthus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Osmanthus fragrans" order="Lamiales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="fragrans">
<emphasis italics="true" pageId="0" pageNumber="113">Osmanthus fragrans</emphasis>
</taxonomicName>
from which our strain was isolated.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="113" type="notes">
<paragraph pageId="0" pageNumber="113">Notes.</paragraph>
<paragraph pageId="0" pageNumber="113">
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe osmanthi</emphasis>
</taxonomicName>
(strain GUCC9165) formed an independent lineage, but was also related to
<taxonomicName lsidName="D. arecae" pageId="0" pageNumber="113" rank="species" species="arecae">
<emphasis italics="true" pageId="0" pageNumber="113">D. arecae</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="D. hongkongensis" pageId="0" pageNumber="113" rank="species" species="hongkongensis">
<emphasis italics="true" pageId="0" pageNumber="113">D. hongkongensis</emphasis>
</taxonomicName>
(
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Parsimonious tree obtained from a combined analyses of an ITS, β - tubulin, calmoudulin and tef 1 sequence dataset. MP, ML above 50 % and BPP values above 0.90 were placed close to topological nodes and separated by &quot; / &quot;. The bootstrap values below 50 % and BPP values below 0.90 were labelled with &quot; - &quot;. The tree is rooted with Valsa ambiens (CFCC 89894). The branch of our new Diaporthe species is in pink." figureDoi="10.3897/mycokeys.57.35448.figure1" httpUri="https://binary.pensoft.net/fig/328111" pageId="0" pageNumber="113">Figure 1</figureCitation>
). The sequences of
<emphasis italics="true" pageId="0" pageNumber="113">
<normalizedToken originalValue="β">β</normalizedToken>
</emphasis>
-tubulin and
<emphasis italics="true" pageId="0" pageNumber="113">tef</emphasis>
1 included about two-three differences between
<taxonomicName lsidName="D. osmanthi" pageId="0" pageNumber="113" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">D. osmanthi</emphasis>
</taxonomicName>
(GUCC9165) and
<taxonomicName lsidName="D. arecae" pageId="0" pageNumber="113" rank="species" species="arecae">
<emphasis italics="true" pageId="0" pageNumber="113">D. arecae</emphasis>
</taxonomicName>
(42) and
<taxonomicName lsidName="D. hongkongensis" pageId="0" pageNumber="113" rank="species" species="hongkongensis">
<emphasis italics="true" pageId="0" pageNumber="113">D. hongkongensis</emphasis>
</taxonomicName>
(78) and thus they were different species according to the guidelines of
<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/7/11/4" author="Jeewon, R" journalOrPublisher="Mycosphere" pageId="0" pageNumber="113" pagination="1669 - 1677" refId="B18" refString="Jeewon, R, Hyde, KD, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. . Mycosphere 7: 1669 - 1677" title="Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities." url="https://doi.org/10.5943/mycosphere/7/11/4" volume="7" year="2016">Jeewon and Hyde (2016)</bibRefCitation>
. Additionally,
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe hongkongensis" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="hongkongensis">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe hongkongensis</emphasis>
</taxonomicName>
produced three types of conidia, but
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe osmanthi</emphasis>
</taxonomicName>
did not produce
<normalizedToken originalValue="γ-conidia">γ-conidia</normalizedToken>
. In addition,
<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
of
<taxonomicName lsidName="D. hongkongensis" pageId="0" pageNumber="113" rank="species" species="hongkongensis">
<emphasis italics="true" pageId="0" pageNumber="113">D. hongkongensis</emphasis>
</taxonomicName>
(18-22
<normalizedToken originalValue="μm">μm</normalizedToken>
) were shorter than those of
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe osmanthi</emphasis>
</taxonomicName>
(
<bibRefCitation DOI="https://doi.org/10.3767/003158513X666844" author="Gomes, RR" journalOrPublisher="Persoonia- Molecular Phylogeny and Evolution of Fungi" pageId="0" pageNumber="113" pagination="1 - 41" refId="B13" refString="Gomes, RR, Glienke, C, Videira, SIR, Lombard, L, Groenewald, JZ, Crous, PW, 2013. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. . Persoonia- Molecular Phylogeny and Evolution of Fungi 31: 1 - 41" title="Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi." url="https://doi.org/10.3767/003158513X666844" volume="31" year="2013">Gomes et al. 2013</bibRefCitation>
). According to original description
<bibRefCitation DOI="https://doi.org/10.1080/00275514.1962.12024974" author="Srivastava, HC" journalOrPublisher="Mycologia" pageId="0" pageNumber="113" pagination="5 - 11" refId="B24" refString="Srivastava, HC, Zakia, B, Govindarajan, VS, 1962. Fruit rot of areca nut caused by a new fungus. . Mycologia 54: 5 - 11" title="Fruit rot of areca nut caused by a new fungus." url="https://doi.org/10.1080/00275514.1962.12024974" volume="54" year="1962">Srivastava et al. (1962)</bibRefCitation>
,
<taxonomicName lsidName="D. arecae" pageId="0" pageNumber="113" rank="species" species="arecae">
<emphasis italics="true" pageId="0" pageNumber="113">D. arecae</emphasis>
</taxonomicName>
also produced two types of conidia. The
<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
(7.2-9.6
<normalizedToken originalValue="×">x</normalizedToken>
2.4
<normalizedToken originalValue="μm">μm</normalizedToken>
) were longer than in
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe osmanthi</emphasis>
</taxonomicName>
, but its
<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
(14.4-24
<normalizedToken originalValue="×">x</normalizedToken>
1.2
<normalizedToken originalValue="μm">μm</normalizedToken>
) were shorter and their shape also had some differences.
</paragraph>
<caption doi="10.3897/mycokeys.57.35448.figure4" httpUri="https://binary.pensoft.net/fig/328114" pageId="0" pageNumber="113" start="Figure 4" startId="F4">
<paragraph pageId="0" pageNumber="113">
Figure 4.
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe osmanthi" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="osmanthi">
<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe osmanthi</emphasis>
</taxonomicName>
(GUCC9165).
<emphasis bold="true" pageId="0" pageNumber="113">
<normalizedToken originalValue="ab">a-b</normalizedToken>
</emphasis>
upper (
<emphasis bold="true" pageId="0" pageNumber="113">a</emphasis>
) and lower (
<emphasis bold="true" pageId="0" pageNumber="113">b</emphasis>
) surface of colony on PDA
<emphasis bold="true" pageId="0" pageNumber="113">
<normalizedToken originalValue="cd">c-d</normalizedToken>
</emphasis>
conidiomata
<emphasis bold="true" pageId="0" pageNumber="113">e</emphasis>
conidiophores, conidiogenous loci and conidia
<emphasis bold="true" pageId="0" pageNumber="113">f</emphasis>
<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
<emphasis bold="true" pageId="0" pageNumber="113">g</emphasis>
two types of conidia
<emphasis bold="true" pageId="0" pageNumber="113">h</emphasis>
<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
. Scale bars: 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="113">e, f, g, h</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>