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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.102.113310" ID-Pensoft-Pub="1314-4049-102-285" ID-Pensoft-UUID="869230B7883257AE86209FB46775D216" ModsDocID="1314-4049-102-285" checkinTime="1709371358857" checkinUser="pensoft" docAuthor="Usman, Muhammad, Dyer, Paul S., Brock, Matthias, Wade, Christopher M. &amp; Khalid, Abdul Nasir" docDate="2024" docId="AB0F169DD7105ACF848B67A78DEAE60E" docLanguage="en" docName="MycoKeys 102: 285-299" docOrigin="MycoKeys 102" docPubDate="2024-03-01" docSource="http://dx.doi.org/10.3897/mycokeys.102.113310" docTitle="Oxneriaria crittendenii Usman &amp; Khalid" docType="treatment" docVersion="1" id="869230B7883257AE86209FB46775D216" lastPageNumber="285" masterDocId="869230B7883257AE86209FB46775D216" masterDocTitle="Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan" masterLastPageNumber="299" masterPageNumber="285" pageNumber="285" updateTime="1709371358857" updateUser="pensoft">
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<mods:title id="89ED856EBAC8F419C8310975E208DB61">Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan</mods:title>
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<mods:namePart id="FE52E87EFFA676976382EBF06EBF9E47">Usman, Muhammad</mods:namePart>
<mods:nameIdentifier id="60D5D344DBB3E64807C815D4F58DBE6B" type="ORCID">https://orcid.org/0000-0002-3490-058X</mods:nameIdentifier>
<mods:affiliation id="BCCE3B99582C05AE54C6A244558A3390">Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus 54590, Lahore, Pakistan &amp; School of Life Sciences, University of Nottingham, Nottingham NG 7 2 RD, UK</mods:affiliation>
<mods:nameIdentifier id="7B5987D788173601330F345793328C10" type="email">musmanmughal52@yahoo.com</mods:nameIdentifier>
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<mods:namePart id="99300BD1E46ADEC7428CACCCB6FD208B">Dyer, Paul S.</mods:namePart>
<mods:nameIdentifier id="52B898869A67BD649CB3943B7C32547E" type="ORCID">https://orcid.org/0000-0003-0237-026X</mods:nameIdentifier>
<mods:affiliation id="7BED16D460C1A2CFD366B47285344833">School of Life Sciences, University of Nottingham, Nottingham NG 7 2 RD, UK</mods:affiliation>
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<mods:namePart id="4E19AE2044DEE61D3EBC5AA324D111AE">Brock, Matthias</mods:namePart>
<mods:nameIdentifier id="0CC6059326B0C4502E2405C6799F0D0D" type="ORCID">https://orcid.org/0000-0003-2774-1856</mods:nameIdentifier>
<mods:affiliation id="2FD688133A8C8F35B02AA9117CA29A60">School of Life Sciences, University of Nottingham, Nottingham NG 7 2 RD, UK</mods:affiliation>
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<mods:namePart id="D118786D317006F50327BA3075C850F2">Wade, Christopher M.</mods:namePart>
<mods:nameIdentifier id="20DE5BF649BE0CA3DC1FE7816F2254EB" type="ORCID">https://orcid.org/0000-0002-1269-9694</mods:nameIdentifier>
<mods:affiliation id="8FD1E3C517C49D92EAEAB939E2DA5023">School of Life Sciences, University of Nottingham, Nottingham NG 7 2 RD, UK</mods:affiliation>
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<mods:namePart id="78115043F4E27B3D04BFEB55A3AC273D">Khalid, Abdul Nasir</mods:namePart>
<mods:nameIdentifier id="675DFD738C841F4809F1EAF2E373F10A" type="ORCID">https://orcid.org/0000-0002-5635-8031</mods:nameIdentifier>
<mods:affiliation id="454C4C93510DD4261052FEB0804B3B48">Fungal Biology and Systematics Research Laboratory, Institute of Botany, University of the Punjab, Quaid-e-Azam Campus 54590, Lahore, Pakistan</mods:affiliation>
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<mods:date id="40D4CB7F62675E2D5DF6710C1712BFF7">2024</mods:date>
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<mods:identifier id="0BE1EF67AF36222B399F16EB064059BA" type="DOI">http://dx.doi.org/10.3897/mycokeys.102.113310</mods:identifier>
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<treatment id="AB0F169DD7105ACF848B67A78DEAE60E" LSID="urn:lsid:plazi:treatment:AB0F169DD7105ACF848B67A78DEAE60E" httpUri="http://treatment.plazi.org/id/AB0F169DD7105ACF848B67A78DEAE60E" lastPageNumber="285" pageId="0" pageNumber="285">
<subSubSection id="2A266056104AB86E96845540B5E91190" pageId="0" pageNumber="285" type="nomenclature">
<paragraph id="F02E927632C62B870314896E14ACC0BC" pageId="0" pageNumber="285">
<taxonomicName id="0A46FAEAE399C321BA78F83E769E5198" LSID="AB0F169D-D710-5ACF-848B-67A78DEAE60E" authority="Usman &amp; Khalid" authorityName="Usman &amp; Khalid" class="Lecanoromycetes" family="Megasporaceae" genus="Oxneriaria" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Oxneriaria crittendenii" order="Pertusariales" pageId="0" pageNumber="285" phylum="Ascomycota" rank="species" species="crittendenii">Oxneriaria crittendenii Usman &amp; Khalid</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection id="304A7232751EB062D77FF0E34AD31520" pageId="0" pageNumber="285" type="description">
<paragraph id="94F253729A53C746A11410250EEC1155" pageId="0" pageNumber="285">
<figureCitation id="3C104FF96F32AD64D8929CB6AA1F83CB" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Oxneriaria crittendenii sp. nov. holotype (LAH 37193) A thallus B margins C apothecia under stereomicroscope D ascospores in Lugol's solution E conidia F pycnidium. Photos by Muhammad Usman. Scale bars: 5 cm (A); 1 mm (B, C); 20 μm (D, E); 100 μm (F)." figureDoi="10.3897/mycokeys.102.113310.figure1" httpUri="https://binary.pensoft.net/fig/995444" pageId="0" pageNumber="285">Fig. 1</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="0FD01781732310D011AD4162A827F40C" pageId="0" pageNumber="285" type="etymology">
<paragraph id="83715671B3B722DC5B206F2B8D470231" pageId="0" pageNumber="285">Etymology.</paragraph>
<paragraph id="3290A249217E6B8ED451C1F2E5A8023E" pageId="0" pageNumber="285">
The specific epithet &quot;
<emphasis id="E60E9948967FD71FDA0CFDD9818DD39F" italics="true" pageId="0" pageNumber="285">crittendenii</emphasis>
&quot; refers to the British lichenologist Prof. Peter D Crittenden in recognition for his outstanding contributions to lichenology.
</paragraph>
</subSubSection>
<subSubSection id="004D2A4AB82201D3EFADF0184CD59C55" pageId="0" pageNumber="285" type="holotype">
<paragraph id="1C7294FC47D206965C6B10047C4FE29B" pageId="0" pageNumber="285">Holotype.</paragraph>
<paragraph id="2506B2DB691F7D10565DAF6301C6A133" pageId="0" pageNumber="285">
Pakistan. Gilgit Baltistan: Deosai Plains (
<geoCoordinate id="F0A04851EB4FEE8AF0690DF1214A419E" degrees="35" direction="north" minutes="0" orientation="latitude" precision="1" seconds="45.73" value="35.012703">35°0'45.73&quot;N</geoCoordinate>
,
<geoCoordinate id="3787677B7A730D9DC8DA26319B1457FD" degrees="75" direction="east" minutes="13" orientation="longitude" precision="1" seconds="25.95" value="75.22388">75°13'25.95&quot;E</geoCoordinate>
, elev. 4,651 m) on rocks, 13 May 2019, M. Usman DEO117 (LAH, holotype; LAH37193). GenBank OR037223 [ITS], OR037219 [LSU], OR037259 [mtSSU].
</paragraph>
</subSubSection>
<subSubSection id="A7E5509DFAE21DBBCD717B8F09414ECA" pageId="0" pageNumber="285" type="diagnosis">
<paragraph id="2B159FD460F332EA8A61089A0874409F" pageId="0" pageNumber="285">Diagnosis.</paragraph>
<paragraph id="D42B52D8AE1976EC0AC5A59A30AD24F8" pageId="0" pageNumber="285">
It differs from its closest species
<taxonomicName id="7D26C58FA03593DA5A9B40CD18E47B7B" family="Megasporaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="O. pruinosa" order="Pertusariales" pageId="0" pageNumber="285" phylum="Ascomycota" rank="species" species="pruinosa">
<emphasis id="7FB037B988F7CEE691B1C4AF4555112C" italics="true" pageId="0" pageNumber="285">O. pruinosa</emphasis>
</taxonomicName>
by having lobate black margins (vs. non-lobate), orbicular thallus 1.5-5 cm (vs. irregular 3-8 cm), K test negative (vs. K positive yellow), distinct proper-exciple 17-40
<normalizedToken id="5A52DB73BC7CFE7FAB9F8ADAAFC53300" originalValue="µm">µm</normalizedToken>
wide (vs. indistinct) and polarilocular ellipsoid ascospores (vs. simple ellipsoid).
</paragraph>
</subSubSection>
<subSubSection id="C11EF555FF9F3CDBA07A15A15323CFEA" pageId="0" pageNumber="285" type="description">
<paragraph id="D77F3D95865A8BCBEF63A9294DB45105" pageId="0" pageNumber="285">Description.</paragraph>
<paragraph id="A32F5F56F5573BCC67D70239272797F1" pageId="0" pageNumber="285">
Thallus crustose, epilithic, orbicular, 1.5-5 cm across, zonate, fine bullate to areolate in the centre to poorly areolate towards margin, in the centre areoles 0.5-1 mm diam. and a few areoles changing to squamules up to 1.8 mm in length, lobate at margins, determinate and radiate.
<emphasis id="A97BDB25E0CDF46EA91901081B5B0B79" bold="true" italics="true" pageId="0" pageNumber="285">Hypothallus</emphasis>
distinct, shiny light brown.
<emphasis id="4E6D5ACA0481BBC46CEC593745CF1E8A" bold="true" italics="true" pageId="0" pageNumber="285">Upper surface</emphasis>
grey with white powdery texture and black at margins.
<emphasis id="A4B0F6001508DD59FF9E20603D022C35" bold="true" italics="true" pageId="0" pageNumber="285">Thallus</emphasis>
heteromerous, upper cortex 20-60
<normalizedToken id="8D6158CB3CBFC75AFA111AA5FB3E9A1D" originalValue="µm">µm</normalizedToken>
thick, globose to sub-globose hyaline paraplectenchymatous cells, 6-11
<normalizedToken id="DA84B51C5867A40A3EC6448283990D42" originalValue="µm">µm</normalizedToken>
in diam.
<emphasis id="99B50E3ED35033F7AE951CB01A173AA8" bold="true" italics="true" pageId="0" pageNumber="285">Algal layer</emphasis>
discontinuous, 90-140
<normalizedToken id="4578D705F1FEC986F0930B5E44583ED4" originalValue="µm">µm</normalizedToken>
thick, photobiont
<taxonomicName id="CA589C4EA9DB93E5E0747B8E441594C0" authorityName="Puymaly" authorityYear="1924" class="Trebouxiophyceae" family="Trebouxiaceae" genus="Trebouxia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Trebouxia" order="Trebouxiales" pageId="0" pageNumber="285" phylum="Chlorophyta" rank="genus">
<emphasis id="E71067F1C4A7AFE064583494BA791BB8" italics="true" pageId="0" pageNumber="285">Trebouxia</emphasis>
</taxonomicName>
sp, coccoid cells, globose to sub-globose 6-14
<normalizedToken id="AB8CF4DC0DC6B771995FCD752048FCF9" originalValue="µm">µm</normalizedToken>
in diam.
<emphasis id="22FBB7770370D48756BB53C286E09761" bold="true" italics="true" pageId="0" pageNumber="285">Medulla</emphasis>
and
<emphasis id="2E4326C8812A3437967B770123178888" bold="true" italics="true" pageId="0" pageNumber="285">lower cortex</emphasis>
not differentiated and consisting of paraplectenchymatous, globose to sub-globose hyaline cells 25-45
<normalizedToken id="B5901EC82BA585B94174B6BBD65BFE78" originalValue="µm">µm</normalizedToken>
in diam.
</paragraph>
<paragraph id="39A34EA3B4BCFAFC7A1C4F8C24D83D9F" pageId="0" pageNumber="285">
<emphasis id="55479573FDFCD57A8DE0483F623A3168" bold="true" italics="true" pageId="0" pageNumber="285">Apothecia</emphasis>
without stipe, aspicilioid, one apothecium per areole, rounded, 600-950
<normalizedToken id="FBDD7B6D1D95A97BDC60BEF8F25A55C4" originalValue="µm">µm</normalizedToken>
in diam., pruinose with black disc 450-700
<normalizedToken id="E16D3E4A55CF783B59686152E6D1E8E2" originalValue="µm">µm</normalizedToken>
, dull and concave.
<emphasis id="07559FE59E82AFDF6F3F223979A7B7CE" bold="true" italics="true" pageId="0" pageNumber="285">Proper exciple</emphasis>
17-40
<normalizedToken id="972476050E40B9E369EE862007D6AA36" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="AF9F3B9A1CE97ED905FF0AFE252A4E46" bold="true" italics="true" pageId="0" pageNumber="285">Thalline exciple</emphasis>
140-190
<normalizedToken id="ECD3903398FB8B0248558B3FB08D135D" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="35C266F2452557B91601D084A709B2AA" bold="true" italics="true" pageId="0" pageNumber="285">Epihymenium</emphasis>
brown, 10-20
<normalizedToken id="1195D76C9FB9AA44CA36F241778C909C" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="9688D8981AA99D1E7D9CA8F9BA7ABD3C" bold="true" italics="true" pageId="0" pageNumber="285">Hymenium</emphasis>
hyaline, 85-110
<normalizedToken id="297B3FAC16970D26D0F5FF33F880209A" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="A02534D90D2E29B29777C70A54BA0FDC" bold="true" italics="true" pageId="0" pageNumber="285">Hypothecium</emphasis>
hyaline, 35-55
<normalizedToken id="AAC58C4C2CAE4151BC0E7DD463B9695B" originalValue="µm">µm</normalizedToken>
thick.
<emphasis id="626780AB2B1FD1AF0CAAD3211A093549" bold="true" italics="true" pageId="0" pageNumber="285">Asci</emphasis>
clavate, 8-spored, 60-100
<normalizedToken id="C9083A2A9326B129D8ACF671B674E8BB" originalValue="×">x</normalizedToken>
22-30
<normalizedToken id="D1029FB309E85B70EA54D6CD3917B3DF" originalValue="µm">µm</normalizedToken>
.
<emphasis id="5BF4A76902A19839B0226E9847895D7E" bold="true" italics="true" pageId="0" pageNumber="285">Ascospores</emphasis>
hyaline, ellipsoid, polarilocular, 13-18
<normalizedToken id="3C0563ED3F12EF17D0B876BCA2AC6E62" originalValue="×">x</normalizedToken>
7-11
<normalizedToken id="6E2FC4C7B4CA8599FF166B630B4EBF1E" originalValue="µm">µm</normalizedToken>
.
<emphasis id="AD8A2845224E35953C4B25DB12FD2422" bold="true" italics="true" pageId="0" pageNumber="285">Paraphyses</emphasis>
moniliform, septate, cylindrical cells 3-10
<normalizedToken id="86E3242C8F1B6773F1924F3CA9648DEB" originalValue="×">x</normalizedToken>
1-2.5
<normalizedToken id="672AFAB6E1F35F990D7F00542FC7C2B1" originalValue="µm">µm</normalizedToken>
, with internally brown terminal cells.
<emphasis id="DC42406A6A82CB2A0BFD7685C1760E02" bold="true" italics="true" pageId="0" pageNumber="285">Pycnidia</emphasis>
roccella type (
<bibRefCitation id="EBDA26D7A05CE25827A7CE6DF5DCAAAF" author="Ryan, BD" journalOrPublisher="Lichenologist (London, England)" pageId="0" pageNumber="285" refId="B20" refString="Ryan, BD, Bungartz, F, Nash, TH, 2002. Morphology and anatomy of the lichen thallus. Lichen flora of the Greater Sonoran Desert Region. I. Lichens unlimited. Arizona State University, Tempe, 8-40." title="Morphology and anatomy of the lichen thallus. Lichen flora of the Greater Sonoran Desert Region. I. Lichens unlimited. Arizona State University, Tempe, 8 - 40." year="2002">Ryan et al. 2002</bibRefCitation>
), globose to pyriform, 115-200
<normalizedToken id="15FE9DFC556F2400619B595E853D13A3" originalValue="×">x</normalizedToken>
85-200
<normalizedToken id="F6AA3FCEBD0942998B83959877E7AD7F" originalValue="µm">µm</normalizedToken>
dark brown ostiole, long filiform hyaline conidia, 17-24
<normalizedToken id="D9A52AE68C61D354A010E29A01842627" originalValue="×">x</normalizedToken>
1
<normalizedToken id="CCB188D4EAA3E90554511E84ABAB36FD" originalValue="µm">µm</normalizedToken>
.
</paragraph>
</subSubSection>
<subSubSection id="6FCB4FD0BDA8041A986ED33336DBC78C" pageId="0" pageNumber="285" type="ecology">
<paragraph id="D9FA2EE7F358132B3FCEA8A2F85A8724" pageId="0" pageNumber="285">Ecology.</paragraph>
<paragraph id="5FD1C1003D374C1A1D7300A6D436F512" pageId="0" pageNumber="285">Saxicolous, calcareous, known only from Deosai Plains, Gilgit-Baltistan, occurring at elevations between 4,117 m and 4,651 m in extremely cold conditions.</paragraph>
</subSubSection>
<subSubSection id="C38DE6976693EFD46DBA2D68F7B55E84" pageId="0" pageNumber="285" type="chemical study">
<paragraph id="470147ECD252FD17D1751115EF378A9B" pageId="0" pageNumber="285">Chemical study:</paragraph>
<paragraph id="3D0B85754EB01205D37BFA1B858235ED" pageId="0" pageNumber="285">
K -ve, C -ve, KC -ve, UV +ve (light green), hymenium initially blue then turning dark orange after
<normalizedToken id="B320EC3783CE5C4BCD3F6436A56231C6" originalValue="Lugols">Lugol's</normalizedToken>
solution. Substictic acid detected through TLC.
</paragraph>
</subSubSection>
<subSubSection id="F7E59BBEEB2E3741FB403DBA535B0E35" pageId="0" pageNumber="285" type="materials_examined">
<paragraph id="A6D7414E556467167425F612CE3F505B" pageId="0" pageNumber="285">Additional material examined.</paragraph>
<paragraph id="D1EB8C6457C69CA2F12F0425DF617857" pageId="0" pageNumber="285">
<materialsCitation id="6CD374776812468125FCD50E32C0B965" collectingDate="2020-09-03" collectorName="M. Usman, M. Shafiq, GenBank OR" country="Pakistan" elevation="4177" latitude="35.12291" location="Deosai Plains" longLatPrecision="1" longitude="75.60975" specimenCount="1" typeStatus="paratype">
<collectingCountry id="2EDD98958E5BFF16258A1D274CFCE591" name="Pakistan">Pakistan</collectingCountry>
. GILGIT BALTISTAN:
<location id="9F9ECAF883B56EB06A7579C5110DC7ED" LSID="urn:lsid:plazi:treatment:AB0F169DD7105ACF848B67A78DEAE60E:9F9ECAF883B56EB06A7579C5110DC7ED" country="Pakistan" latitude="35.12291" longLatPrecision="1" longitude="75.60975" name="Deosai Plains">Deosai Plains</location>
,
<geoCoordinate id="67D30174DAB55949EB9EB53001DDB15C" degrees="35" direction="north" minutes="7" orientation="latitude" precision="1" seconds="22.48" value="35.12291">35°7'22.48&quot;N</geoCoordinate>
,
<geoCoordinate id="69225E1A743C538C01BC6261C73D5545" degrees="75" direction="east" minutes="36" orientation="longitude" precision="1" seconds="35.09" value="75.60975">75°36'35.09&quot;E</geoCoordinate>
, elev.
<quantity id="A9E2C3961A7882F0342B4A1E9000ECAA" metricMagnitude="3" metricUnit="m" metricValue="4.177" unit="m" value="4177.0">
<elevation id="B732194271BF0740BC9FF4D8A57B059F" metricMagnitude="3" metricUnit="m" metricValue="4.177" unit="m" value="4177.0">4,177 m</elevation>
</quantity>
, on rocks,
<collectingDate id="1B7DF85FE298CAF530FE35D7D2E25F35" value="2020-09-03">3 September 2020</collectingDate>
,
<collectorName id="63FB9985D56095784C62B0AB3FED9DB1">M. Usman</collectorName>
&amp;
<collectorName id="9FEB3C7FCD6147F72639E8D07AC0D3E0">M. Shafiq</collectorName>
DEO129 (LAH,
<typeStatus id="C78866CCB71DC6AC92267B459661B14D" type="paratype">paratype</typeStatus>
; LAH37194;
<collectorName id="C6799468A832227C00EBDD08C0304550">GenBank OR</collectorName>
037224 [ITS], OR037220 [LSU], OR037260 [mtSSU].
</materialsCitation>
</paragraph>
</subSubSection>
</treatment>
</document>