130 lines
14 KiB
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130 lines
14 KiB
XML
<document id="D41C12AE98EA457BA2B9558D6654405E" ID-DOI="10.1016/j.phytochem.2021.112847" ID-ISSN="1873-3700" ID-Zenodo-Dep="8273033" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="julia" IM.metadata_approvedBy="julia" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1692303169482" checkinUser="felipe" docAuthor="Fan, Honghong, Wei, Guo, Chen, Xinlu, Guo, Hong, Crandall-Stotler, Barbara, Kollner, Tobias G. & Chen, Feng" docDate="2021" docId="AA6F87ECFFA54B0D61393FEAFA64FACE" docLanguage="en" docName="Phytochemistry.190.112847.pdf" docOrigin="Phytochemistry (112847) 190" docSource="http://dx.doi.org/10.1016/j.phytochem.2021.112847" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Radula lindenbergiana" docType="treatment" docVersion="1" lastPageNumber="2" masterDocId="5656FF94FFA44B0C620B3D0AFFE9FF97" masterDocTitle="Sesquiterpene biosynthesis in a leafy liverwort Radula lindenbergiana Gottsche ex C. Hartm" masterLastPageNumber="8" masterPageNumber="1" pageNumber="2" updateTime="1692729354687" updateUser="julia">
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<mods:title id="4FAD90C885A2C86F50B90E07D0DF68E3">Sesquiterpene biosynthesis in a leafy liverwort Radula lindenbergiana Gottsche ex C. Hartm</mods:title>
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<mods:namePart id="52B6F1F367EEA1DC93B0FEB202FEAD2A">Fan, Honghong</mods:namePart>
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<mods:affiliation id="8E18FB9516BB14E5A6A087A8FB9F7806">* & Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA & School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China</mods:affiliation>
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<mods:namePart id="F737A4BB21923093D19D483D1BD6D521">Wei, Guo</mods:namePart>
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<mods:namePart id="A16418E01B414BA08375AF02CD6EF7C5">Chen, Xinlu</mods:namePart>
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<mods:namePart id="9CAA60AE5F4CB922F7ECB7AACAFDAB03">Guo, Hong</mods:namePart>
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<mods:namePart id="6048D7FD369D3EAE5D32B4ADF443A2C6">Crandall-Stotler, Barbara</mods:namePart>
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<mods:namePart id="6A55B1CEA4F9F9DE8BA3D8E940B9EFFA">Kollner, Tobias G.</mods:namePart>
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<mods:namePart id="58630145D771D2E0C68D96376327D1B8">Chen, Feng</mods:namePart>
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<mods:title id="F91DC7A2B3D4FBF905F051059E32636E">Phytochemistry</mods:title>
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<mods:date id="72D11A09B82289EA14564B633089F720">2021</mods:date>
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<mods:title id="D6EE873E0923FE16493FE8F249EEA80C">112847</mods:title>
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<mods:number id="455085C783236D76D971CE5FAD4F42F4">2021-10-31</mods:number>
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<mods:number id="BBA1DB81E0FFD252732196668A74722B">190</mods:number>
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<mods:url id="F2001FC7556AF66BF82A5C5547F39FFB">http://dx.doi.org/10.1016/j.phytochem.2021.112847</mods:url>
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<mods:classification id="8C6F2582F6A0DC96EE1D617201579BEE">journal article</mods:classification>
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<mods:identifier id="F5CB239D442DF7591D8601B6DF98F804" type="DOI">10.1016/j.phytochem.2021.112847</mods:identifier>
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<mods:identifier id="E27ADFCB33BEFAB95AE15419F4B86BA1" type="ISSN">1873-3700</mods:identifier>
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<treatment id="AA6F87ECFFA54B0D61393FEAFA64FACE" LSID="urn:lsid:plazi:treatment:AA6F87ECFFA54B0D61393FEAFA64FACE" httpUri="http://treatment.plazi.org/id/AA6F87ECFFA54B0D61393FEAFA64FACE" lastPageNumber="2" pageId="1" pageNumber="2">
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<subSubSection id="6ADC6571FFA54B0D61393FEAFC53FC98" pageId="1" pageNumber="2" type="nomenclature">
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<paragraph id="227936FAFFA54B0D61393FEAFC53FC98" blockId="1.[818,1347,736,755]" lastBlockId="1.[818,954,764,783]" pageId="1" pageNumber="2">
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<heading id="79318196FFA54B0D61393FEAFAAAFD64" bold="true" box="[818,1347,736,755]" fontSize="36" level="1" pageId="1" pageNumber="2" reason="1">
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<emphasis id="10B2EAE8FFA54B0D61393FEAFAC1FD64" bold="true" box="[818,1320,736,755]" italics="true" pageId="1" pageNumber="2">2.2. Terpene-producing genes from the transcriptome of</emphasis>
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<taxonomicName id="E5C64D79FFA54B0D67243FEAFAAAFD64" ID-CoL="4R9QX" ID-ENA="697108" authority="Gottsche ex C. Hartm" box="[1327,1347,736,755]" class="Jungermanniopsida" family="Radulaceae" genus="Radula" kingdom="Plantae" order="Porellales" pageId="1" pageNumber="2" phylum="Marchantiophyta" rank="species" species="lindenbergiana">
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<emphasis id="10B2EAE8FFA54B0D67243FEAFAAAFD64" bold="true" box="[1327,1347,736,755]" italics="true" pageId="1" pageNumber="2">R.</emphasis>
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</taxonomicName>
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</heading>
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<heading id="79318196FFA54B0D61393FF6FC53FC98" box="[818,954,764,783]" fontSize="8" level="3" pageId="1" pageNumber="2" reason="8">
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<emphasis id="10B2EAE8FFA54B0D61393FF6FC53FC98" bold="true" box="[818,954,764,783]" italics="true" pageId="1" pageNumber="2">Lindenbergiana</emphasis>
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</heading>
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</paragraph>
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<paragraph id="227936FAFFA54B0D615A3E3EFC48FB02" blockId="1.[818,1489,819,1369]" pageId="1" pageNumber="2">
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To identify candidate terpene-producing genes in
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<taxonomicName id="E5C64D79FFA54B0D67273E3EFA20FCD1" authority="Gottsche ex C. Hartm" box="[1324,1481,819,839]" class="Jungermanniopsida" family="Radulaceae" genus="Radula" kingdom="Plantae" order="Porellales" pageId="1" pageNumber="2" phylum="Marchantiophyta" rank="species" species="lindenbergiana">
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<emphasis id="10B2EAE8FFA54B0D67273E3EFA20FCD1" bold="true" box="[1324,1481,819,839]" italics="true" pageId="1" pageNumber="2">R. lindenbergiana</emphasis>
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</taxonomicName>
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, its transcriptomes from the One thousand plant (OneKP) project (https: //sites.google.com/a/ualberta.ca/onekp/) were searched for
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<emphasis id="10B2EAE8FFA54B0D67743E66FA4AFCE8" bold="true" box="[1407,1443,876,895]" italics="true" pageId="1" pageNumber="2">TPS</emphasis>
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and
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<emphasis id="10B2EAE8FFA54B0D61393E8DFC9AFC0D" bold="true" box="[818,883,903,922]" italics="true" pageId="1" pageNumber="2">MTPLS</emphasis>
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genes using a HMMER method (
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<bibRefCitation id="46574B0BFFA54B0D66A23E8DFAACFC0D" author="Finn, R. D. & Clements, J. & Miller, B. L. & Wheeler, T. J. & Schreiber, F. & Bateman, A. & Eddy, S. R." box="[1193,1349,903,923]" pageId="1" pageNumber="2" pagination="38" refId="ref7865" refString="Finn, R. D., Clements, J., Arndt, W., Miller, B. L., Wheeler, T. J., Schreiber, F., Bateman, A., Eddy, S. R., 2015. HMMER web server: 2015 update. Nucleic Acids Res. 43, W 30 - W 38. https: // doi. org / 10.1093 / nar / gkv 397." type="journal article" year="2015">Finn et al., 2015</bibRefCitation>
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). A single TPS homolog designated as RlTPS1 was identified. RlTPS1 appears to be partial sequence. The protein it encodes is most similar to MpDTPS5 from
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<taxonomicName id="E5C64D79FFA54B0D616D3ED1FC06FC79" box="[870,1007,987,1006]" class="Marchantiopsida" family="Marchantiaceae" genus="Marchantia" kingdom="Plantae" order="Marchantiales" pageId="1" pageNumber="2" phylum="Marchantiophyta" rank="species" species="polymorpha">
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<emphasis id="10B2EAE8FFA54B0D616D3ED1FC06FC79" bold="true" box="[870,1007,987,1006]" italics="true" pageId="1" pageNumber="2">M. polymorpha</emphasis>
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</taxonomicName>
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(Supplemental
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<figureCitation id="BAFD2A7FFFA54B0D668C3ED1FB08FC79" box="[1159,1249,987,1006]" captionStart="Fig" captionStartId="1.[818,848,1786,1803]" captionTargetBox="[824,1483,1580,1757]" captionTargetId="figure-1138@1.[823,1484,1579,1758]" captionTargetPageId="1" captionText="Fig. 1. Chemical profiling of R. lindenbergiana. 5, striatene; 6, pentalenene; 8, α-funebrene; 9, β-elemene; 10, petasitene; 11, α-cedrene; 12, isobazzanene; 13, (E)-α-bergamotene; 14, epi-β-santalene; 15, (E)-β-farnesene; 17, β-santalene; 18, β-acoradiene; 19, 4,5-di-epi-aristolochene; 20, ar-curcumene; 21, unidentified sesquiterpene; 22, eremophilene; 23, α-selinene; 24, (E,E)-α-farnesene and β-curcumene; 25, (Z)-β-bisabolene; All listed compounds are tentatively identified using mass spec libraries. All numbered compounds that are not listed represent unidentified sesquiterpenes." figureDoi="http://doi.org/10.5281/zenodo.8273035" httpUri="https://zenodo.org/record/8273035/files/figure.png" pageId="1" pageNumber="2">Figure S1</figureCitation>
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). Because MpDTPS5 is a putative diterpene synthase (Bowman et al., 2017), we hypothesized that RlTPS1 is also a diterpene synthase. In contrast, a total of 12
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<emphasis id="10B2EAE8FFA54B0D67853919FA26FBB1" bold="true" box="[1422,1487,1043,1062]" italics="true" pageId="1" pageNumber="2">MTPSL</emphasis>
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genes were found. Five of them that appeared to be full-length genes were designated
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<emphasis id="10B2EAE8FFA54B0D61DE3940FBA1FBCA" bold="true" box="[981,1096,1098,1117]" italics="true" pageId="1" pageNumber="2">RlMTPSL1-5</emphasis>
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. A cDNA for each of the five genes was cloned by reverse transcription PCR (RT-PCR) and verified by sequencing.
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</paragraph>
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<paragraph id="227936FAFFA54B0D615A3994FA64FACE" blockId="1.[818,1489,819,1369]" pageId="1" pageNumber="2">
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RlMTPSL1-5 encode proteins of 391–441 amino acids in length (
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<figureCitation id="BAFD2A7FFFA54B0D613139B0FC95FB5A" box="[826,892,1210,1229]" captionStart="Fig" captionStartId="1.[818,848,1786,1803]" captionTargetBox="[824,1483,1580,1757]" captionTargetId="figure-1138@1.[823,1484,1579,1758]" captionTargetPageId="1" captionText="Fig. 1. Chemical profiling of R. lindenbergiana. 5, striatene; 6, pentalenene; 8, α-funebrene; 9, β-elemene; 10, petasitene; 11, α-cedrene; 12, isobazzanene; 13, (E)-α-bergamotene; 14, epi-β-santalene; 15, (E)-β-farnesene; 17, β-santalene; 18, β-acoradiene; 19, 4,5-di-epi-aristolochene; 20, ar-curcumene; 21, unidentified sesquiterpene; 22, eremophilene; 23, α-selinene; 24, (E,E)-α-farnesene and β-curcumene; 25, (Z)-β-bisabolene; All listed compounds are tentatively identified using mass spec libraries. All numbered compounds that are not listed represent unidentified sesquiterpenes." figureDoi="http://doi.org/10.5281/zenodo.8273035" httpUri="https://zenodo.org/record/8273035/files/figure.png" pageId="1" pageNumber="2">Fig. S1</figureCitation>
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). Sequence similarities among the five RlMTPSLs range from 38% (RlMTPSL1 and RlMTPS4) to 71% (RlMTPSL2 and SlMTPSL3). Sequence alignment showed that they had
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<subScript id="BE4234BFFFA54B0D66FD39F9FADEFA91" attach="right" box="[1270,1335,1267,1288]" fontSize="6" pageId="1" pageNumber="2">DDXXD</subScript>
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and NSE/DTE conserved motifs, which were reported to be involved in ionization of the substrate and catalysis (
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<bibRefCitation id="46574B0BFFA54B0D66463820FB17FAAA" author="Chen, F. & Tholl, D. & Bohlmann, J. & Pichersky, E." box="[1101,1278,1322,1341]" pageId="1" pageNumber="2" pagination="212 - 229" refId="ref7323" refString="Chen, F., Tholl, D., Bohlmann, J., Pichersky, E., 2011. The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom. Plant J. 66, 212 - 229. https: // doi. org / 10.1111 / j. 1365 - 313 X. 2011.04520. x." type="journal article" year="2011">Chen et al., 2011</bibRefCitation>
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). GenBank accession numbers for the five
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<emphasis id="10B2EAE8FFA54B0D61F3384FFBA5FACF" bold="true" box="[1016,1100,1349,1368]" italics="true" pageId="1" pageNumber="2">RlMTPSL</emphasis>
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genes are MZ367362-MZ367366.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |