124 lines
11 KiB
XML
124 lines
11 KiB
XML
<document id="BAEDC5D30EE014C43F9C2AE9D60E65F7" ID-DOI="10.1016/j.phytochem.2021.112847" ID-ISSN="1873-3700" ID-Zenodo-Dep="8273033" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="julia" IM.metadata_approvedBy="julia" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1692303169482" checkinUser="felipe" docAuthor="Fan, Honghong, Wei, Guo, Chen, Xinlu, Guo, Hong, Crandall-Stotler, Barbara, Kollner, Tobias G. & Chen, Feng" docDate="2021" docId="AA6F87ECFFA24B0A626F3EFAFD7AFA6E" docLanguage="en" docName="Phytochemistry.190.112847.pdf" docOrigin="Phytochemistry (112847) 190" docSource="http://dx.doi.org/10.1016/j.phytochem.2021.112847" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Escherichia coli BL" docType="treatment" docVersion="1" lastPageNumber="7" masterDocId="5656FF94FFA44B0C620B3D0AFFE9FF97" masterDocTitle="Sesquiterpene biosynthesis in a leafy liverwort Radula lindenbergiana Gottsche ex C. Hartm" masterLastPageNumber="8" masterPageNumber="1" pageNumber="7" updateTime="1692729354687" updateUser="julia">
|
||
<mods:mods id="A9A3C630E858799FC88D805D33A2AFBE" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="2C73304F1AD835D128C1DB9E519C9108">
|
||
<mods:title id="FA179FC8CD231FF713AA6B73DDF89620">Sesquiterpene biosynthesis in a leafy liverwort Radula lindenbergiana Gottsche ex C. Hartm</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="1C70E7BC80E989C508BE42FB959353A0" type="personal">
|
||
<mods:role id="ACE4F62142E917DA2E57AD59BDD8EB01">
|
||
<mods:roleTerm id="8DD298A38090AC01BB650B86E96598C1">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="F841488B13FD8504D74AFF5B75A355D0">Fan, Honghong</mods:namePart>
|
||
<mods:affiliation id="B725986C83A74E55BD59303B1FAE612D">* & Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996, USA & School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="F3DFBB85270A19065FB166E4C71A79E1" type="personal">
|
||
<mods:role id="89E2E421DE191EFCBB861757F56FACB5">
|
||
<mods:roleTerm id="F18DE3644D62C03E8F15B0D7095CDC5F">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="F4AD8A3F1D1361CC27B73577F50F95FE">Wei, Guo</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="8943C8918D5AB47B8CEDABB457255722" type="personal">
|
||
<mods:role id="616B6C9BCEC471956351F60A561E25B5">
|
||
<mods:roleTerm id="F990B256F1A3CA5E80C27D960E543D84">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="30448759EB939EB75135E61992D7A23E">Chen, Xinlu</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="BD69854526AA0E6ADA08B30679B09827" type="personal">
|
||
<mods:role id="30832A0049E81FF25FC25E61AC6E60D2">
|
||
<mods:roleTerm id="785EC22B6EFB06C47091E39DBDFC1CAA">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="5861CF96C56B0A5D5BFF29C157215D11">Guo, Hong</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="9A3672A2B7AC2EADEEA7097E633DC65D" type="personal">
|
||
<mods:role id="3CAB82DA02D2859C9EE7E50087041C0A">
|
||
<mods:roleTerm id="5AE7902CD5C380B435CEE8A4135A34DA">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="0958FB8BF92EB14765D714E0169C9FB8">Crandall-Stotler, Barbara</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="EFFDB2E935AA248DAF1F22FF66EA35A2" type="personal">
|
||
<mods:role id="FBF4E1C8A9BAF08467252F8A803B6FEE">
|
||
<mods:roleTerm id="1C5A14DE859F95ABB853D66ED95A1DA2">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="A0FDE47FAF3390D1F3D4164EE319FDBF">Kollner, Tobias G.</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="7B6236D12431D7899E6FE1833D46D7B3" type="personal">
|
||
<mods:role id="551D8FF68DB1010E3776BCB6CD3CE651">
|
||
<mods:roleTerm id="2887B3B85BB58CA3D70A2C244A21CD06">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="CB461162EC040DAE6726F9EB831CDA28">Chen, Feng</mods:namePart>
|
||
</mods:name>
|
||
<mods:typeOfResource id="A43A0FC1B3C90528694890F81C1E0C91">text</mods:typeOfResource>
|
||
<mods:relatedItem id="98BA54961F97D720F0AFC170BE0D2DEB" type="host">
|
||
<mods:titleInfo id="17418FE2E1D99A5EB76C44AFBF5C5662">
|
||
<mods:title id="07EC28C8B3D37773FAD540331A705992">Phytochemistry</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="556E670D21A4EBBB1A10D8171F55EFF0">
|
||
<mods:date id="B3535960D60A8C23C7BE9AECAEA3EB0C">2021</mods:date>
|
||
<mods:detail id="40C0C5EAFEC7D2B1FE07320FF3966659" type="series">
|
||
<mods:title id="A90FE7E7CEE71924CA864AD983B9B162">112847</mods:title>
|
||
</mods:detail>
|
||
<mods:detail id="6DDF9A2FD851222D75DD1E4182B2A39D" type="pubDate">
|
||
<mods:number id="1887D403F25B7A1680EF55C5E8F5253F">2021-10-31</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="2B050A82A75086CE6FFE4DBB5486FBBF" type="volume">
|
||
<mods:number id="CAD61E8AAF1556BA623CDE65DB0653F4">190</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="4A45FBFED16D1CC9027FE6572CB216EB" unit="page">
|
||
<mods:start id="E72DF4A7A67CCF91742CCA13F80ED1F4">1</mods:start>
|
||
<mods:end id="50382E381089908B7D8DFDF6E664DD80">8</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location id="C40F3013F62C57B1571A78C9B96279BC">
|
||
<mods:url id="E811BAECB858DF06357D9E940A5DCB1B">http://dx.doi.org/10.1016/j.phytochem.2021.112847</mods:url>
|
||
</mods:location>
|
||
<mods:classification id="C67799641BF7F0D4DA41CC88EC1DFC31">journal article</mods:classification>
|
||
<mods:identifier id="BE0D062FE3070AB23585302A383C4D8C" type="DOI">10.1016/j.phytochem.2021.112847</mods:identifier>
|
||
<mods:identifier id="8FD9996D1D6BBE8F6924FCFEA1668776" type="ISSN">1873-3700</mods:identifier>
|
||
<mods:identifier id="21516C3E4DE25A4F4C176568BC1314C6" type="Zenodo-Dep">8273033</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="AA6F87ECFFA24B0A626F3EFAFD7AFA6E" LSID="urn:lsid:plazi:treatment:AA6F87ECFFA24B0A626F3EFAFD7AFA6E" httpUri="http://treatment.plazi.org/id/AA6F87ECFFA24B0A626F3EFAFD7AFA6E" lastPageNumber="7" pageId="6" pageNumber="7">
|
||
<subSubSection id="6ADC6571FFA24B0A626F3EFAFD81FB94" box="[100,616,1008,1027]" pageId="6" pageNumber="7" type="nomenclature">
|
||
<paragraph id="227936FAFFA24B0A626F3EFAFD81FB94" blockId="6.[100,616,1008,1027]" box="[100,616,1008,1027]" pageId="6" pageNumber="7">
|
||
<heading id="79318196FFA24B0A626F3EFAFD81FB94" bold="true" box="[100,616,1008,1027]" fontSize="36" level="1" pageId="6" pageNumber="7" reason="1">
|
||
<emphasis id="10B2EAE8FFA24B0A626F3EFAFD81FB94" bold="true" box="[100,616,1008,1027]" italics="true" pageId="6" pageNumber="7">
|
||
5.4. cDNA cloning and heterologous expression in
|
||
<taxonomicName id="E5C64D79FFA24B0A603B3EFAFD81FB94" ID-CoL="3BGTD" ID-ENA="1010795" authority="BL" authorityName="BL" box="[560,616,1008,1027]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="6" pageNumber="7" phylum="Proteobacteria" rank="species" species="coli">E. coli</taxonomicName>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="6ADC6571FFA24B0A628F3922FD7AFA6E" pageId="6" pageNumber="7" type="description">
|
||
<paragraph id="227936FAFFA24B0A628F3922FD7AFA6E" blockId="6.[100,770,1063,1529]" pageId="6" pageNumber="7">
|
||
Plants of
|
||
<taxonomicName id="E5C64D79FFA24B0A62D63922FE90FBAD" authority="Gottsche ex C. Hartm" box="[221,377,1063,1083]" class="Jungermanniopsida" family="Radulaceae" genus="Radula" kingdom="Plantae" order="Porellales" pageId="6" pageNumber="7" phylum="Marchantiophyta" rank="species" species="lindenbergiana">
|
||
<emphasis id="10B2EAE8FFA24B0A62D63922FE90FBAD" bold="true" box="[221,377,1063,1083]" italics="true" pageId="6" pageNumber="7">R. lindenbergiana</emphasis>
|
||
</taxonomicName>
|
||
were harvested for RNA extraction using the QIAGEN RNA isolation Kit. RNA was then converted to single-strand cDNA by GE First-strand cDNA synthesis Kit. The
|
||
<emphasis id="10B2EAE8FFA24B0A60353955FD7BFBE5" bold="true" box="[574,658,1119,1138]" italics="true" pageId="6" pageNumber="7">RlMTPSL</emphasis>
|
||
genes were amplified by RT-PCR using the primers listed in Supplemental
|
||
<tableCitation id="6F440341FFA24B0A60A03971FD15FB18" box="[683,764,1147,1167]" captionStart="Table 1" captionStartId="2.[100,150,150,166]" captionTargetPageId="2" captionText="Table 1 Terpene profile of vegetative plants of Radula lindenbergiana." httpUri="http://table.plazi.org/id/76B96672FFA64B0E626F3D9CFD81FF57" pageId="6" pageNumber="7" tableUuid="76B96672FFA64B0E626F3D9CFD81FF57">Table S1</tableCitation>
|
||
. The amplification products were cloned into the protein expression vector pEXP5-CT/TOPO (Invitrogen) and were confirmed by sequencing. Protein expression constructs were transferred into
|
||
<taxonomicName id="E5C64D79FFA24B0A60C239C5FF68FB69" authority="BL" authorityName="BL" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="6" pageNumber="7" phylum="Proteobacteria" rank="species" species="coli">
|
||
<emphasis id="10B2EAE8FFA24B0A60C239C5FCEBFB75" bold="true" box="[713,770,1231,1250]" italics="true" pageId="6" pageNumber="7">E. coli</emphasis>
|
||
BL
|
||
</taxonomicName>
|
||
21-Codon Plus (DE3) for protein expression and the bacteria were cultured in LB medium at 37
|
||
<superScript id="D5B39BB2FFA24B0A638E380EFE64FA86" attach="left" box="[389,397,1284,1297]" fontSize="6" pageId="6" pageNumber="7">◦</superScript>
|
||
until OD
|
||
<subScript id="BE4234BFFFA24B0A63E73804FDEEFA8B" attach="left" box="[492,519,1294,1308]" fontSize="6" pageId="6" pageNumber="7">600</subScript>
|
||
reached 0.4–0.6. The recombinant proteins were expressed for 16 h at 18
|
||
<superScript id="D5B39BB2FFA24B0A6032382AFDA8FABA" attach="right" box="[569,577,1312,1325]" fontSize="6" pageId="6" pageNumber="7">◦</superScript>
|
||
C by adding 0.4 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG). The cells were collected by centrifugation at
|
||
<quantity id="E53E9B1FFFA24B0A632B3851FEB0FAF9" box="[288,345,1371,1390]" metricMagnitude="0" metricUnit="kg" metricValue="4.0" pageId="6" pageNumber="7" unit="g" value="4000.0">
|
||
4000
|
||
<emphasis id="10B2EAE8FFA24B0A635B3851FEB0FAF9" bold="true" box="[336,345,1371,1390]" italics="true" pageId="6" pageNumber="7">g</emphasis>
|
||
</quantity>
|
||
for 10 min. After that, they were disrupted by a 8 × 10 s treatment with a sonicator (Misonix Microson ultrasonic cell disruptor,
|
||
<collectingCountry id="5AD1766AFFA24B0A62CC3898FF1BFA32" box="[199,242,1426,1445]" name="United States of America" pageId="6" pageNumber="7">USA</collectingCountry>
|
||
) in chilled extraction buffer [50 mM Tris⋅HCl, pH 7.5, 5 mM DTT, 0.5 mM phenylmethylsulfonyl fluoride (PMSF), 10% (vol/vol) glycerol]. Cell fragments were removed by centrifugation at
|
||
<quantity id="E53E9B1FFFA24B0A609A38C0FD0BFA49" box="[657,738,1482,1502]" metricMagnitude="1" metricUnit="kg" metricValue="1.4" pageId="6" pageNumber="7" unit="g" value="14000.0">14,000 g</quantity>
|
||
for 30 min and the supernatant was desalted into assay buffer.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |