266 lines
18 KiB
XML
266 lines
18 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.85.75944" ID-GBIF-Dataset="5d20d456-e1c2-421e-b44b-f26f6ba9aaff" ID-PMC="PMC8674231" ID-Pensoft-Pub="1314-4049-85-57" ID-Pensoft-UUID="54BBF1B5057C580B9C3C4BF9357FEB3F" ID-PubMed="34975280" ModsDocID="1314-4049-85-57" checkinTime="1639023500839" checkinUser="pensoft" docAuthor="Mu, Taichang, Zhang, Zhaoxue, Liu, Rongyu, Liu, Shubin, Li, Zhuang, Zhang, Xiuguo & Xia, Jiwen" docDate="2021" docId="8B12D86AEE2759998CF70A098B4F8771" docLanguage="en" docName="MycoKeys 85: 57-71" docOrigin="MycoKeys 85" docPubDate="2021-12-08" docSource="http://dx.doi.org/10.3897/mycokeys.85.75944" docTitle="Colletotrichum mengyinense T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="54BBF1B5057C580B9C3C4BF9357FEB3F" lastPageNumber="57" masterDocId="54BBF1B5057C580B9C3C4BF9357FEB3F" masterDocTitle="Morphological and molecular phylogenetic analyses reveal three species of Colletotrichum in Shandong province, China" masterLastPageNumber="71" masterPageNumber="57" pageNumber="57" updateTime="1668136769803" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular phylogenetic analyses reveal three species of Colletotrichum in Shandong province, China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Mu, Taichang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Zhaoxue</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Liu, Rongyu</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Liu, Shubin</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">zhenjunxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-12-08</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>85</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>57</mods:start>
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<mods:end>71</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.85.75944</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.85.75944</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-85-57</mods:identifier>
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<mods:identifier type="Pensoft-UUID">54BBF1B5057C580B9C3C4BF9357FEB3F</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="191259090" LSID="urn:lsid:plazi:treatment:8B12D86AEE2759998CF70A098B4F8771" httpUri="http://treatment.plazi.org/id/8B12D86AEE2759998CF70A098B4F8771" lastPageNumber="57" pageId="0" pageNumber="57">
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<subSubSection pageId="0" pageNumber="57" type="nomenclature">
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<paragraph pageId="0" pageNumber="57">
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<taxonomicName LSID="8B12D86A-EE27-5999-8CF7-0A098B4F8771" authority="T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li" authorityName="T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum mengyinense" order="Glomerellales" pageId="0" pageNumber="57" phylum="Ascomycota" rank="species" species="mengyinense" status="sp. nov.">Colletotrichum mengyinense T.C. Mu, J.W. Xia, X.G. Zhang & Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="57">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="description">
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<paragraph pageId="0" pageNumber="57">
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Colletotrichum mengyinense (SAUCC 200702) a branch with leaves of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 7 days on PDA d conidiomata e-g conidiophores, conidiogenous cells and conidia h-j conidia. Scale bars: 10 μm (e-j)." figureDoi="10.3897/mycokeys.85.75944.figure3" httpUri="https://binary.pensoft.net/fig/620454" pageId="0" pageNumber="57">Figure 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="etymology">
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<paragraph pageId="0" pageNumber="57">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="57">Named after Mengyin County where the fungus was collected.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="diagnosis">
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<paragraph pageId="0" pageNumber="57">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="57">
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<taxonomicName authorityName="T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum mengyinense" order="Glomerellales" pageId="0" pageNumber="57" phylum="Ascomycota" rank="species" species="mengyinense">
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<emphasis italics="true" pageId="0" pageNumber="57">Colletotrichum mengyinense</emphasis>
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</taxonomicName>
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can be distinguished from the phylogenetically most closely related species
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<taxonomicName lsidName="C. fructicola" pageId="0" pageNumber="57" rank="species" species="fructicola">
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<emphasis italics="true" pageId="0" pageNumber="57">C. fructicola</emphasis>
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</taxonomicName>
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(MFLU 090228) by its large conidia (12.5-15.7
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<normalizedToken originalValue="×">x</normalizedToken>
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4.8-6.1 vs. 9.7-14.0
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<normalizedToken originalValue="×">x</normalizedToken>
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3.0-4.3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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), and five loci (2/509 in the ITS region, 1/139 GAPDH, 9/237 ACT, 8/410 TUB2 and 20/727 GS).
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="materials_examined">
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<paragraph pageId="0" pageNumber="57">
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<typeStatus>Type</typeStatus>
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.
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</paragraph>
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<paragraph pageId="0" pageNumber="57">
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<materialsCitation collectingDate="2020-07-25" collectorName="T. C. Mu" country="China" county="Mengyin County" location="Mengyin County" specimenCount="1" stateProvince="Shandong Province" typeStatus="holotype">
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<collectingCountry name="China">China</collectingCountry>
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,
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<collectingRegion country="China" name="Shandong">Shandong Province</collectingRegion>
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:
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<collectingCounty>Mengyin County</collectingCounty>
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, on diseased leaves of
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<taxonomicName class="Magnoliopsida" family="Rosaceae" genus="Rosa" kingdom="Plantae" lsidName="Rosa chinensis" order="Rosales" pageId="0" pageNumber="57" phylum="Tracheophyta" rank="species" species="chinensis">
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<emphasis italics="true" pageId="0" pageNumber="57">Rosa chinensis</emphasis>
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</taxonomicName>
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,
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<collectingDate value="2020-07-25">25 July 2020</collectingDate>
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,
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<collectorName>T.C. Mu</collectorName>
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,
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<typeStatus>holotype</typeStatus>
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HSAUP200702, ex-type living culture SAUCC200702
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="description">
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<paragraph pageId="0" pageNumber="57">Description.</paragraph>
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<paragraph pageId="0" pageNumber="57">
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Leaf spots discoid to irregular, brown or tanned. Asexual morph developed on SNA. A yellowish or orange mass appearing just as accumulations of conidia on the surface of the medium of SNA after 14 days in light at 25 °C. Conidia one-celled, hyaline, smooth-walled, subcylindrical, both ends round, contents granular. Conidia on SNA (12.5-15.7
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<normalizedToken originalValue="×">x</normalizedToken>
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4.8-6.1
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, mean
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<normalizedToken originalValue="±">+/-</normalizedToken>
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SD = 14.3
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<normalizedToken originalValue="±">+/-</normalizedToken>
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1.1
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<normalizedToken originalValue="×">x</normalizedToken>
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5.3
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<normalizedToken originalValue="±">+/-</normalizedToken>
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0.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, L/W ratio = 2.7, n = 40). Sexual morph not observed. Conidiogenous cells subcylindrical, hyaline, 5.3-15.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.9-4.9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, opening 1.7-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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diam. Conidiophores hyaline, smooth walled, septate, branched.
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</paragraph>
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<caption doi="10.3897/mycokeys.85.75944.figure3" httpUri="https://binary.pensoft.net/fig/620454" pageId="0" pageNumber="57" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="57">
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<emphasis bold="true" pageId="0" pageNumber="57">Figure 3.</emphasis>
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<taxonomicName authorityName="T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum mengyinense" order="Glomerellales" pageId="0" pageNumber="57" phylum="Ascomycota" rank="species" species="mengyinense">
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<emphasis italics="true" pageId="0" pageNumber="57">Colletotrichum mengyinense</emphasis>
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</taxonomicName>
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(SAUCC200702)
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<emphasis bold="true" pageId="0" pageNumber="57">a</emphasis>
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branch with leaves of host plant
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<emphasis bold="true" pageId="0" pageNumber="57">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="57">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="57">c</emphasis>
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) sides of colony after incubation for 7 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="57">d</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="57">e-g</emphasis>
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conidiophores, conidiogenous cells and conidia
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<emphasis bold="true" pageId="0" pageNumber="57">h-j</emphasis>
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conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="57">e-j</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="description">
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<paragraph pageId="0" pageNumber="57">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="57">Colonies on PDA flat with entire margin, aerial mycelium white or gray, floccose cottony; surface and reverse gray in the center and grayish margin. PDA attaining 69.3-75.6 mm in diameter after 7 days, at 25 °C, growth rate 9.9-10.8 mm/day. Colonies on SNA sparse hyphae, slow growth.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="additional specimen examined">
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<paragraph pageId="0" pageNumber="57">Additional specimen examined.</paragraph>
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<paragraph pageId="0" pageNumber="57">
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China, Shandong Province: Mengyin County, on diseased fruit of
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<taxonomicName class="Magnoliopsida" family="Juglandaceae" genus="Juglans" kingdom="Plantae" lsidName="Juglans regia" order="Fagales" pageId="0" pageNumber="57" phylum="Tracheophyta" rank="species" species="regia">
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<emphasis italics="true" pageId="0" pageNumber="57">Juglans regia</emphasis>
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</taxonomicName>
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, 25 July 2020, T.C. Mu, paratype HSAUP200912, ex-paratype living culture SAUCC200912. China, Shandong Province: Mengyin County, on diseased fruit of
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<taxonomicName class="Magnoliopsida" family="Juglandaceae" genus="Juglans" kingdom="Plantae" lsidName="Juglans regia" order="Fagales" pageId="0" pageNumber="57" phylum="Tracheophyta" rank="species" species="regia">
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<emphasis italics="true" pageId="0" pageNumber="57">Juglans regia</emphasis>
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</taxonomicName>
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, 25 July 2020, T.C. Mu, paratype HSAUP200913, ex-paratype living culture SAUCC200913. China, Shandong Province: Mengyin County, on diseased fruit of
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<taxonomicName class="Magnoliopsida" family="Juglandaceae" genus="Juglans" kingdom="Plantae" lsidName="Juglans regia" order="Fagales" pageId="0" pageNumber="57" phylum="Tracheophyta" rank="species" species="regia">
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<emphasis italics="true" pageId="0" pageNumber="57">Juglans regia</emphasis>
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</taxonomicName>
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, 25 July 2020, T.C. Mu, paratype HSAUP200983, ex-paratype living culture SAUCC200983.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="57" type="notes">
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<paragraph pageId="0" pageNumber="57">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="57">
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Phylogenetic analysis of a combined seven gene showed that
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<taxonomicName authorityName="T. C. Mu, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum mengyinense" order="Glomerellales" pageId="0" pageNumber="57" phylum="Ascomycota" rank="species" species="mengyinense">
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<emphasis italics="true" pageId="0" pageNumber="57">Colletotrichum mengyinense</emphasis>
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</taxonomicName>
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formed an independent clade (Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Colletotrichum gloeosporioides complex based on combined ITS, GAPDH, CHS- 1, ACT, TUB 2, CAL and GS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Two branches were shortened to fit the page size-these are indicated by the symbol (//) with an indication number showing how many times they are shortened." figureDoi="10.3897/mycokeys.85.75944.figure1" httpUri="https://binary.pensoft.net/fig/620452" pageId="0" pageNumber="57">1</figureCitation>
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) and is phylogenetically distinct from
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<taxonomicName lsidName="C. fructicola" pageId="0" pageNumber="57" rank="species" species="fructicola">
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<emphasis italics="true" pageId="0" pageNumber="57">C. fructicola</emphasis>
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</taxonomicName>
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(
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<bibRefCitation author="Prihastuti, H" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="57" pagination="89 - 109" refId="B20" refString="Prihastuti, H, Cai, L, Chen, H, Mckenzie, EHC, Hyde, KD, 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39: 89 - 109" title="Characterization of Colletotrichum species associated with coffee berries in northern Thailand." volume="39" year="2009">Prihastuti et al. 2009</bibRefCitation>
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). This species can be distinguished from
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<taxonomicName lsidName="C. fructicola" pageId="0" pageNumber="57" rank="species" species="fructicola">
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<emphasis italics="true" pageId="0" pageNumber="57">C. fructicola</emphasis>
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</taxonomicName>
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by 40 different nucleotides (2/509 in the ITS region, 1/139 in the GAPDH region, 9/237 ACT, 8/410 TUB2 and 20/727 GS).
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<normalizedToken originalValue="What’s">What's</normalizedToken>
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more,
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<taxonomicName lsidName="C. mengyinense" pageId="0" pageNumber="57" rank="species" species="mengyinense">
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<emphasis italics="true" pageId="0" pageNumber="57">C. mengyinense</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="C. fructicola" pageId="0" pageNumber="57" rank="species" species="fructicola">
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<emphasis italics="true" pageId="0" pageNumber="57">C. fructicola</emphasis>
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</taxonomicName>
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in having large conidia (12.5-15.7
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<normalizedToken originalValue="×">x</normalizedToken>
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4.8-6.1 vs. 9.7-14.0
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<normalizedToken originalValue="×">x</normalizedToken>
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3.0-4.3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, mean: 14.3
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<normalizedToken originalValue="×">x</normalizedToken>
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5.3 vs. 11.53
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<normalizedToken originalValue="×">x</normalizedToken>
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3.55
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). Therefore, we establish this fungus as a novel species.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |