213 lines
13 KiB
XML
213 lines
13 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.49.31904" ID-GBIF-Dataset="48b57ed4-7970-4cf9-b12d-75e2de91a2ac" ID-PMC="PMC6422929" ID-Pensoft-Pub="1314-4049-49-1" ID-PubMed="30914842" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049-49-1" ModsDocOrigin="MycoKeys 49" ModsDocTitle="Two new endophytic Colletotrichum species from Nothapodytespittosporoides in China" checkinTime="1555333417461" checkinUser="pensoft" docAuthor="Zhou, Sixuan, Qiao, Lijun, Jayawardena, Ruvishika S., Hyde, Kevin D., Xiaoya Ma,, Wen, Tingchi & Kang, Jichuan" docDate="2019" docId="7EF3D2082123AB370622B847AA1F4843" docLanguage="en" docName="MycoKeys 49: 1-14" docOrigin="MycoKeys 49" docSource="http://dx.doi.org/10.3897/mycokeys.49.31904" docTitle="Colletotrichum tongrenense S. X. Zhou, J. C. Kang & K. D. Hyde, sp. nov." docType="treatment" docVersion="4" lastPageNumber="1" masterDocId="FFA0F9714C38FFAEFF8AFF88FFEB4E0C" masterDocTitle="Two new endophytic Colletotrichum species from Nothapodytespittosporoides in China" masterLastPageNumber="14" masterPageNumber="1" pageNumber="1" updateTime="1668136185306" updateUser="ExternalLinkService">
|
||
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo>
|
||
<mods:title>Two new endophytic Colletotrichum species from Nothapodytespittosporoides in China</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Zhou, Sixuan</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Qiao, Lijun</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Hyde, Kevin D.</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Xiaoya Ma,</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Wen, Tingchi</mods:namePart>
|
||
</mods:name>
|
||
<mods:name type="personal">
|
||
<mods:role>
|
||
<mods:roleTerm>Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart>Kang, Jichuan</mods:namePart>
|
||
</mods:name>
|
||
<mods:typeOfResource>text</mods:typeOfResource>
|
||
<mods:relatedItem type="host">
|
||
<mods:titleInfo>
|
||
<mods:title>MycoKeys</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part>
|
||
<mods:date>2019</mods:date>
|
||
<mods:detail type="volume">
|
||
<mods:number>49</mods:number>
|
||
</mods:detail>
|
||
<mods:extent unit="page">
|
||
<mods:start>1</mods:start>
|
||
<mods:end>14</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location>
|
||
<mods:url>http://dx.doi.org/10.3897/mycokeys.49.31904</mods:url>
|
||
</mods:location>
|
||
<mods:classification>journal article</mods:classification>
|
||
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.49.31904</mods:identifier>
|
||
<mods:identifier type="Pensoft-Pub">1314-4049-49-1</mods:identifier>
|
||
</mods:mods>
|
||
<treatment ID-GBIF-Taxon="156202031" LSID="urn:lsid:plazi:treatment:7EF3D2082123AB370622B847AA1F4843" httpUri="http://treatment.plazi.org/id/7EF3D2082123AB370622B847AA1F4843" lastPageNumber="1" pageId="0" pageNumber="1">
|
||
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
|
||
<paragraph pageId="0" pageNumber="1">
|
||
<taxonomicName LSID="828725" authority="S. X. Zhou, J. C. Kang & K. D. Hyde" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tongrenense" order="Glomerellales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="tongrenense">Colletotrichum tongrenense S.X. Zhou, J.C. Kang & K.D. Hyde</taxonomicName>
|
||
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
|
||
Fig. 3
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection pageId="0" pageNumber="1" type="etymology">
|
||
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">
|
||
<normalizedToken originalValue="‘tongrenense’">'tongrenense'</normalizedToken>
|
||
referring Tongren City, site of collection of type species.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection pageId="0" pageNumber="1" type="description">
|
||
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">
|
||
Endophytic in leaves and stems of
|
||
<taxonomicName class="Magnoliopsida" family="Icacinaceae" genus="Nothapodytes" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Nothapodytes pittosporoides" order="Icacinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="pittosporoides">Nothapodytes pittosporoides</taxonomicName>
|
||
. Sexual morph: Undetermined. Asexual morph: On WA, vegetative hyphae 1.4-6
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
diam. (n=10), smooth-walled, septate, branched, hyaline.
|
||
<taxonomicName genus="Chlamydospores" lsidName="Chlamydospores" pageId="0" pageNumber="1" rank="genus">Chlamydospores</taxonomicName>
|
||
not observed. Setae unbranched, septate, tapering to rounded at apical end, pale brown to dark brown, smooth-walled, 45-90
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
long, 5.9-6.2
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
wide at widest part, 2.6-5.8
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
wide at bottom, 1.5-1.6
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
wide at apex. Conidiophores pale brown, septate, branched. Conidiogenous cells pale, hyaline, smooth-walled, erect, clavate or cylindrical, 2-11
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
1-2
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(x‒ = 6.3
|
||
<normalizedToken originalValue="±">+/-</normalizedToken>
|
||
4.4
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
1.7
|
||
<normalizedToken originalValue="±">+/-</normalizedToken>
|
||
0.4
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
, n = 20), L/W ratio= 3.7. Conidia hyaline, aseptate, smooth-walled, variable in size and shape, thick-walled, ellipsoidal to subglobose, the apex and base rounded, slightly constricted in the middle, 11-14
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
5-7
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(x‒ = 13.1
|
||
<normalizedToken originalValue="±">+/-</normalizedToken>
|
||
1.0
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
5.5
|
||
<normalizedToken originalValue="±">+/-</normalizedToken>
|
||
0.6
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
, n = 40), L/W ratio= 2.4.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection pageId="0" pageNumber="1" type="description">
|
||
<paragraph pageId="0" pageNumber="1">Culture characteristics.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">Cultures on WA at 25 °C in darkness, reaching 15-18 mm diam. in 21 days, white to grey, asymmetrical surface, reverse dark grey to black.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">Colonies on PDA at 25 °C reaching 45-55 mm diam. in 12 days in darkness, circular, more or less planar, surface dark brown, covered with abundant, pale grey, lanose to cottony aerial mycelium, margin smooth, entire and pale white. Reverse dark grey, margin pale white.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">Cultures on CMA, 10-15 mm diam. in 21 days, covered with dark brown aerial mycelium, sparse, reverse light brown, margin irregular.</paragraph>
|
||
</subSubSection>
|
||
<subSubSection pageId="0" pageNumber="1" type="materials_examined">
|
||
<paragraph pageId="0" pageNumber="1">Material examined.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">
|
||
CHINA, Guizhou province, Tongren (
|
||
<geoCoordinate direction="north" orientation="latitude" precision="15" value="27.593613">27°35'37"N</geoCoordinate>
|
||
,
|
||
<geoCoordinate direction="east" orientation="longitude" precision="15" value="109.18278">109°10'58"E</geoCoordinate>
|
||
, elevation 332.8 m), isolated from healthy stems of
|
||
<taxonomicName class="Magnoliopsida" family="Icacinaceae" genus="Nothapodytes" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Nothapodytes pittosporoides" order="Icacinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="pittosporoides">Nothapodytes pittosporoides</taxonomicName>
|
||
, 27 May 2016, S.X. Zhou and L.J. Qiao (Holotype GACP GZU-TRJ1-37 dried culture), ex-type living culture, GMBC0209.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection pageId="0" pageNumber="1" type="notes">
|
||
<paragraph pageId="0" pageNumber="1">Notes.</paragraph>
|
||
<paragraph pageId="0" pageNumber="1">
|
||
<taxonomicName class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tongrenense" order="Glomerellales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="tongrenense">Colletotrichum tongrenense</taxonomicName>
|
||
belongs to the
|
||
<taxonomicName lsidName="C. dracaenophilum" pageId="0" pageNumber="1" rank="species" species="dracaenophilum">C. dracaenophilum</taxonomicName>
|
||
species complex (
|
||
<bibRefCitation author="Damm, U" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="1 - 46" title="The Colletotrichumdracaenophilum, C. magnum and C. orchidearum species complexes." url="https://doi.org/10.1016/j.simyco.2018.04.001" volume="92" year="2019">Damm et al. 2019</bibRefCitation>
|
||
). Morphologically,
|
||
<taxonomicName lsidName="C. tongrenense" pageId="0" pageNumber="1" rank="species" species="tongrenense">C. tongrenense</taxonomicName>
|
||
resembles
|
||
<taxonomicName lsidName="C. tropicicola" pageId="0" pageNumber="1" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
|
||
and
|
||
<taxonomicName lsidName="C. excelsum-altitudum" pageId="0" pageNumber="1" rank="species" species="excelsum-altitudum">C. excelsum-altitudum</taxonomicName>
|
||
in conidia characters, but it can be distinguished from
|
||
<taxonomicName lsidName="C. tropicicola" pageId="0" pageNumber="1" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
|
||
in having setae and longer conidia (15-19
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
vs 11-14
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
) (
|
||
<bibRefCitation author="Noireung, P" journalOrPublisher="Cryptogamie, Mycologie," pageId="0" pageNumber="1" pagination="347 - 362" title="Novel species of Colletotrichum revealed by morphology and molecular analysis." url="https://doi.org/10.7872/crym.v33.iss3.2012.347" volume="33" year="2012">Noireung et al. 2012</bibRefCitation>
|
||
).
|
||
<taxonomicName lsidName="C. tongrenense" pageId="0" pageNumber="1" rank="species" species="tongrenense">C. tongrenense</taxonomicName>
|
||
is distinguished from
|
||
<taxonomicName lsidName="C. excelsum-altitudum" pageId="0" pageNumber="1" rank="species" species="excelsum-altitudum">C. excelsum-altitudum</taxonomicName>
|
||
(
|
||
<bibRefCitation author="Tao, G" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="1" pagination="139 - 164" title="Endophytic Colletotrichum species from Bletillaochracea (Orchidaceae), with descriptions of seven new species." url="https://doi.org/10.1007/s13225-013-0254-5" volume="61" year="2013">Tao et al. 2013</bibRefCitation>
|
||
) in having smaller conidiophores (2-11
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
1-2
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
vs 8.5-25
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
4-5
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
). Phylogenetically, the new isolate GZU_TRJ1-37 clusters together with
|
||
<taxonomicName lsidName="C. tropicicola" pageId="0" pageNumber="1" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
|
||
with good bootstrap support (94% MLBS, 1.00 PP) (Fig. 1) and the phylogenetic analysis supports it as a distinct species. There are 6, 4, 2 and 5 base pairs differences in ITS, TUB2, ACT and GAPDH gene regions, respectively, between the new isolate and the type strain of
|
||
<taxonomicName lsidName="C. tropicicola" pageId="0" pageNumber="1" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
|
||
, which confirms that they are separate species. Therefore, it is introduced as a novel species.
|
||
</paragraph>
|
||
<caption pageId="0" pageNumber="1">
|
||
<paragraph pageId="0" pageNumber="1">
|
||
Figure 3.
|
||
<taxonomicName class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum tongrenense" order="Glomerellales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="tongrenense">Colletotrichum tongrenense</taxonomicName>
|
||
(GACP GZU_TRJ1-37, holotype) a, b colonies on
|
||
<normalizedToken originalValue="WAc–g">WAc-g</normalizedToken>
|
||
Conidiophores
|
||
<normalizedToken originalValue="h–l">h-l</normalizedToken>
|
||
Conidia. Scale bars: 40
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
(c), 20
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
(d, g), 10
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
(e, f), 10
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
(
|
||
<normalizedToken originalValue="h–l">h-l</normalizedToken>
|
||
).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |